MD12G1046400.v1.1

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007394
Physical Location & Seq
Reverse (-)
5207209 .. 5210706
3498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1046400.v1.1.491

Sequence Viewer

Length: 1518 bp
ATGGATTCAGAACCATTTCCATCCTCAAGGCCGACGGTGAAATGTTGTAATTGTGAATGTAGTTGTTCGTTGGTGAGTGTTTCTTCAGGGGGGACTTGGCTTCGGTCTGTTAAGAGAAAGTATGGCGAGTTTGAGGATGGTAACCGTTTCTTTATACCCGGGATTGAACTTTATTATAATGCACGAGTCCAAATGGAGAACGAATGTGTTGCACTACGCGAGACGCTCAGTAGCCAGCAGAACACGATACAAGATTTGTATACTGAGCTGGACGAGGAGAGGAATGCTTCATCATCCGCTGCCAATGAGGCTATGTCAATGATATTGAGGTTGCAGGGGGAGAAGGCTGAAATACAGATGGAGGCCCGGCAATTCAAGAGTTTCGTTGAGGAGAAGATGGCACATGACCAGCAGGAGCTTTTGGCACTGGAAGATTTTCTGTATAAGAGAGAACAGGCGATTCAGGCACTCACGTGTGAAGTGCAGGCCTATAAGCATAGAATGATGAGTTATGGGCTCACGGAGTCTGAGGCAGAAGGTGAGAATGGTTCCTTTAGCCGTATCCAGAGTGTGGGTGACTTTGAAGCCCAGTATGAACTTCCAACAACGCCCACATATGAATACCCACCTTTGAAATGTAAAATAAATGAGACCCAAGGTGCGTTGGAGTGTGATGATGATGCAGATGTTGAGAAATATGCATTTGGTGAGACCCCTCGTGGGAGAGAGCATTTGAGAAATTTGGAAAGCAGGCTCCATCAGATGGAGAGGAGTCCGAGTGGCAGTTCGATGGGAGGGGATTTTTCAGGTTCCAAGAACATTTTTGAAAAGGTGGTGGTTGGCCATTCTCCTCGGCGGTCAAGACATTCCGGAAGACTTTCAAGTGACTCACCTATGTATACGGTTAAAGAAACTGGTCCAGATTTTGCTGAATCTCCTAGATATAACAGCAGCTTCAGGAAGGCAGACTATGTCTCACAGTCTGAGGACTACCCAAATACAAGAAAGTTTGATAATATATCAGAAGTTGGAGATGACACGAGTGATAGAATTTATACCATTGACTCTGTTCATAACGGAGCACCTTACAATGGTTATACAGAAGCCAAATCTGGAGTAACTCCGAGGGGACTTACTGGGCCTGATTTTGAGGATCCTGATGTCATGAAGCTCTATATGAGGCTTCAGGCACTTGAGGCTGACAGGGAGTCAATGAGGCAGGCAATGCTTTCTATGCGAACAGATAAAGCTCAACTGGTATTGCTTAAAGAAATAGCTCAACATTTGTGTAAAGATATGTCACCGGAGAAACAAATGATTGTGAAGAAGCCATCTCCCGTTAGAAGCTTTTCTTTCATTTCTGTTTTTAAGTGGATCATGTCCTTTGTTTTCTGGAGAAGGAGGGCACGCCGAAGCAAGCATATGTTTGGGCTATCAGCCAATGTGGGTTTGCTTATGCTTCTAGACAAGGGTCCCCATGTAAGGCAGTGGAGATGTCTTACAAGTACACAAGTGTAA

Protein Analysis

506

Amino Acids

57.59

Weight (kDa)

5.75

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 71 - 161 4.4e-35 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AccB7I CCANNNNNTGG 2 cut(s) 571, 763
AccI GTMKAC 2 cut(s) 260, 899
AccII CGCG 1 cut(s) 219
AccIII TCCGGA 1 cut(s) 869
AciI CCGC 2 cut(s) 297, 856
AclWI GGATC 3 cut(s) 1148, 1161, 1382
AcoI YGGCCR 1 cut(s) 841
AcsI RAATTY 2 cut(s) 739, 1050
AcuI CTGAAG 3 cut(s) 69, 940, 1169
AcvI CACGTG 1 cut(s) 474
AfaI GTAC 1 cut(s) 1507
AfiI CCNNNNNNNGG 5 cut(s) 571, 720, 763, 1091, 1482
AflIII ACRYGT 1 cut(s) 473
AgsI TTSAA 6 cut(s) 167, 376, 584, 634, 827, 882
AhdI GACNNNNNGTC 1 cut(s) 1207
AleI CACNNNNGTG 1 cut(s) 472
AluBI AGCT 7 cut(s) 268, 418, 954, 1171, 1250, 1277, 1347
AluI AGCT 7 cut(s) 268, 418, 954, 1171, 1250, 1277, 1347
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 4 cut(s) 215, 644, 704, 979
AlwI GGATC 3 cut(s) 1148, 1161, 1382
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 869
AoxI GGCC 5 cut(s) 29, 363, 486, 841, 1139
ApeKI GCWGC 2 cut(s) 299, 951
ApoI RAATTY 2 cut(s) 739, 1050
Asp700I GAANNNNTTC 4 cut(s) 15, 435, 877, 1348
AspS9I GGNCC 4 cut(s) 364, 917, 1139, 1472
AsuC2I CCSGG 3 cut(s) 159, 160, 367
AsuHPI GGTGA 7 cut(s) 49, 85, 551, 587, 719, 882, 1293
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 2 cut(s) 917, 1472
BaeGI GKGCMC 1 cut(s) 1408
BalI TGGCCA 1 cut(s) 843
BamHI GGATCC 1 cut(s) 1153
BanII GRGCYC 1 cut(s) 519
BauI CACGAG 3 cut(s) 183, 717, 1039
BbrPI CACGTG 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 880
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 2 cut(s) 286, 963
BccI CCATC 8 cut(s) 28, 131, 352, 391, 757, 765, 784, 1339
BceAI ACGGC 1 cut(s) 543
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BciVI GTATCC 1 cut(s) 572
BcnI CCSGG 3 cut(s) 159, 160, 367
BcoDI GTCTC 4 cut(s) 215, 644, 704, 979
BfaI CTAG 2 cut(s) 939, 1463
BfuI GTATCC 1 cut(s) 572
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 2 cut(s) 300, 952
BlsI GCNGC 2 cut(s) 301, 953
Bme1390I CCNGG 3 cut(s) 159, 160, 367
Bme18I GGWCC 2 cut(s) 917, 1472
BmeRI GACNNNNNGTC 1 cut(s) 1207
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 4 cut(s) 364, 917, 1139, 1472
BmiI GGNNCC 6 cut(s) 550, 755, 811, 1155, 1473, 1474
BmrFI CCNGG 3 cut(s) 159, 160, 367
BmrI ACTGGG 2 cut(s) 583, 1146
BmsI GCATC 1 cut(s) 670
BmuI ACTGGG 2 cut(s) 583, 1146
BoxI GACNNNNGTC 1 cut(s) 1470
BpiI GAAGAC 1 cut(s) 880
BpmI CTGGAG 2 cut(s) 1134, 1414
BpuEI CTTGAG 2 cut(s) 10, 1214
BpuMI CCSGG 3 cut(s) 159, 160, 367
BsaAI YACGTR 1 cut(s) 474
BsaI GGTCTC 2 cut(s) 644, 704
BsaJI CCNNGG 4 cut(s) 158, 655, 851, 1124
BsaWI WCCGGW 2 cut(s) 869, 1303
BsaXI ACNNNNNCTCC 2 cut(s) 763, 793
Bsc4I CCNNNNNNNGG 5 cut(s) 571, 720, 763, 1091, 1482
Bse1I ACTGG 5 cut(s) 432, 589, 919, 1141, 1260
Bse3DI GCAATG 1 cut(s) 1230
BseAI TCCGGA 1 cut(s) 869
BseDI CCNNGG 4 cut(s) 158, 655, 851, 1124
BseGI GGATG 3 cut(s) 20, 142, 293
BseLI CCNNNNNNNGG 5 cut(s) 571, 720, 763, 1091, 1482
BseMI GCAATG 1 cut(s) 1230
BseMII CTCAG 4 cut(s) 241, 255, 519, 975
BseNI ACTGG 5 cut(s) 432, 589, 919, 1141, 1260
BseRI GAGGAG 4 cut(s) 290, 404, 784, 840
BseSI GKGCMC 1 cut(s) 1408
BseXI GCAGC 2 cut(s) 286, 963
BsgI GTGCAG 1 cut(s) 503
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 5 cut(s) 31, 365, 488, 843, 1141
BsiHKAI GWGCWC 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 4 cut(s) 159, 367, 870, 1304
BslFI GGGAC 3 cut(s) 106, 1143, 1458
BslI CCNNNNNNNGG 5 cut(s) 571, 720, 763, 1091, 1482
BsmAI GTCTC 4 cut(s) 215, 644, 704, 979
BsmBI CGTCTC 1 cut(s) 215
BsmFI GGGAC 3 cut(s) 106, 1143, 1458
BsmI GAATGC 1 cut(s) 289
BsnI GGCC 5 cut(s) 31, 365, 488, 843, 1141
Bso31I GGTCTC 2 cut(s) 644, 704
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 3 cut(s) 519, 1084, 1408
Bsp13I TCCGGA 1 cut(s) 869
Bsp143I GATC 2 cut(s) 1153, 1374
BspACI CCGC 2 cut(s) 297, 856
BspANI GGCC 5 cut(s) 31, 365, 488, 843, 1141
BspCNI CTCAG 4 cut(s) 240, 256, 520, 976
BspEI TCCGGA 1 cut(s) 869
BspFNI CGCG 1 cut(s) 219
BspHI TCATGA 1 cut(s) 1164
BspLI GGNNCC 6 cut(s) 550, 755, 811, 1155, 1473, 1474
BspPI GGATC 3 cut(s) 1148, 1161, 1382
BspTNI GGTCTC 2 cut(s) 644, 704
BsrDI GCAATG 1 cut(s) 1230
BsrI ACTGG 5 cut(s) 432, 589, 919, 1141, 1260
BssECI CCNNGG 4 cut(s) 158, 655, 851, 1124
BssMI GATC 2 cut(s) 1153, 1374
BssNAI GTATAC 2 cut(s) 261, 900
BssSI CACGAG 3 cut(s) 183, 717, 1039
BssT1I CCWWGG 1 cut(s) 655
Bst1107I GTATAC 2 cut(s) 261, 900
Bst2BI CACGAG 3 cut(s) 183, 717, 1039
Bst4CI ACNGT 4 cut(s) 37, 146, 904, 981
BstAPI GCANNNNNTGC 1 cut(s) 1225
BstBAI YACGTR 1 cut(s) 474
BstC8I GCNNGC 6 cut(s) 236, 486, 752, 1221, 1408, 1418
BstDEI CTNAG 4 cut(s) 227, 264, 528, 984
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 3 cut(s) 20, 142, 293
BstFNI CGCG 1 cut(s) 219
BstKTI GATC 2 cut(s) 1156, 1377
BstMAI GTCTC 4 cut(s) 215, 644, 704, 979
BstMBI GATC 2 cut(s) 1153, 1374
BstMWI GCNNNNNNNGC 5 cut(s) 308, 464, 1196, 1225, 1234
BstPAI GACNNNNGTC 1 cut(s) 1470
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 3 cut(s) 157, 158, 365
BstSLI GKGCMC 1 cut(s) 1408
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 286, 963
BstV2I GAAGAC 1 cut(s) 880
BstX2I RGATCY 1 cut(s) 1153
BstYI RGATCY 1 cut(s) 1153
BstZ17I GTATAC 2 cut(s) 261, 900
BsuI GTATCC 1 cut(s) 572
BsuRI GGCC 5 cut(s) 31, 365, 488, 843, 1141
BtsCI GGATG 3 cut(s) 20, 142, 293
BtsI GCAGTG 1 cut(s) 1493
BtsIMutI CAGTG 2 cut(s) 425, 1493
Cac8I GCNNGC 6 cut(s) 236, 486, 752, 1221, 1408, 1418
CciI TCATGA 1 cut(s) 1164
Cfr13I GGNCC 4 cut(s) 364, 917, 1139, 1472
Cfr9I CCCGGG 1 cut(s) 158
CseI GACGC 1 cut(s) 232
Csp6I GTAC 1 cut(s) 1506
CviAII CATG 4 cut(s) 404, 1165, 1378, 1478
CviQI GTAC 1 cut(s) 1506
DdeI CTNAG 4 cut(s) 227, 264, 528, 984
DpnI GATC 2 cut(s) 1155, 1376
DpnII GATC 2 cut(s) 1153, 1374
DriI GACNNNNNGTC 1 cut(s) 1207
EaeI YGGCCR 1 cut(s) 841
Eam1105I GACNNNNNGTC 1 cut(s) 1207
Eco130I CCWWGG 1 cut(s) 655
Eco147I AGGCCT 1 cut(s) 488
Eco24I GRGCYC 1 cut(s) 519
Eco31I GGTCTC 2 cut(s) 644, 704
Eco47I GGWCC 2 cut(s) 917, 1472
Eco57I CTGAAG 3 cut(s) 69, 940, 1169
Eco72I CACGTG 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 158
Eco91I GGTNACC 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 1472
EcoO65I GGTNACC 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 655
EcoT22I ATGCAT 1 cut(s) 703
EcoT38I GRGCYC 1 cut(s) 519
ErhI CCWWGG 1 cut(s) 655
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 4 cut(s) 407, 1168, 1381, 1481
FalI AAGNNNNNCTT 2 cut(s) 1336, 1368
FaqI GGGAC 3 cut(s) 106, 1143, 1458
FatI CATG 4 cut(s) 403, 1164, 1377, 1477
FauNDI CATATG 2 cut(s) 616, 1422
FblI GTMKAC 2 cut(s) 260, 899
Fnu4HI GCNGC 2 cut(s) 300, 952
FokI GGATG 3 cut(s) 7, 149, 280
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 2 cut(s) 300, 952
FspBI CTAG 2 cut(s) 939, 1463
GluI GCNGC 2 cut(s) 300, 952
GsuI CTGGAG 2 cut(s) 1134, 1414
HaeIII GGCC 5 cut(s) 31, 365, 488, 843, 1141
HapII CCGG 4 cut(s) 159, 367, 870, 1304
HgaI GACGC 1 cut(s) 232
Hin1II CATG 4 cut(s) 407, 1168, 1381, 1481
HindIII AAGCTT 1 cut(s) 1345
HinfI GANTC 9 cut(s) 5, 186, 460, 524, 772, 887, 932, 1064, 1208
HpaII CCGG 4 cut(s) 159, 367, 870, 1304
HphI GGTGA 7 cut(s) 49, 85, 551, 587, 719, 882, 1293
Hpy166II GTNNAC 3 cut(s) 261, 900, 1508
Hpy188I TCNGA 7 cut(s) 10, 529, 762, 777, 985, 1024, 1125
Hpy8I GTNNAC 3 cut(s) 261, 900, 1508
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 4 cut(s) 337, 530, 955, 1392
HpyCH4III ACNGT 4 cut(s) 37, 146, 904, 981
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 6 cut(s) 182, 212, 334, 484, 683, 701
HpyF10VI GCNNNNNNNGC 5 cut(s) 308, 464, 1196, 1225, 1234
HpyF3I CTNAG 4 cut(s) 227, 264, 528, 984
HpySE526I ACGT 1 cut(s) 473
Hsp92II CATG 4 cut(s) 407, 1168, 1381, 1481
KflI GGGWCCC 1 cut(s) 1472
Kpn2I TCCGGA 1 cut(s) 869
Kzo9I GATC 2 cut(s) 1153, 1374
LmnI GCTCC 3 cut(s) 415, 759, 1079
Lsp1109I GCAGC 2 cut(s) 286, 963
LweI GCATC 1 cut(s) 670
MaeI CTAG 2 cut(s) 939, 1463
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 5 cut(s) 140, 575, 884, 1117, 1299
MalI GATC 2 cut(s) 1155, 1376
MboI GATC 2 cut(s) 1153, 1374
MboII GAAGA 5 cut(s) 75, 406, 443, 885, 1336
MflI RGATCY 1 cut(s) 1153
MhlI GDGCHC 3 cut(s) 519, 1084, 1408
MlsI TGGCCA 1 cut(s) 843
MluCI AATT 4 cut(s) 49, 371, 739, 1050
MluNI TGGCCA 1 cut(s) 843
MlyI GAGTC 6 cut(s) 195, 533, 781, 881, 1058, 1217
MmeI TCCRAC 3 cut(s) 626, 645, 1009
Mox20I TGGCCA 1 cut(s) 843
Mph1103I ATGCAT 1 cut(s) 703
MroI TCCGGA 1 cut(s) 869
MroXI GAANNNNTTC 4 cut(s) 15, 435, 877, 1348
MscI TGGCCA 1 cut(s) 843
MseI TTAA 4 cut(s) 111, 906, 1266, 1368
MslI CAYNNNNRTG 1 cut(s) 472
Msp20I TGGCCA 1 cut(s) 843
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 4 cut(s) 159, 367, 870, 1304
MspR9I CCNGG 3 cut(s) 159, 160, 367
Mva1269I GAATGC 1 cut(s) 289
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 5 cut(s) 308, 464, 1196, 1225, 1234
NciI CCSGG 3 cut(s) 159, 160, 367
NdeI CATATG 2 cut(s) 616, 1422
NdeII GATC 2 cut(s) 1153, 1374
NlaIII CATG 4 cut(s) 407, 1168, 1381, 1481
NlaIV GGNNCC 6 cut(s) 550, 755, 811, 1155, 1473, 1474
NmeAIII GCCGAG 1 cut(s) 832
NmuCI GTSAC 3 cut(s) 575, 884, 1299
NsiI ATGCAT 1 cut(s) 703
OliI CACNNNNGTG 1 cut(s) 472
PagI TCATGA 1 cut(s) 1164
PceI AGGCCT 1 cut(s) 488
PctI GAATGC 1 cut(s) 289
PdmI GAANNNNTTC 4 cut(s) 15, 435, 877, 1348
PfeI GAWTC 3 cut(s) 5, 460, 932
PflFI GACNNNGTC 1 cut(s) 971
PflMI CCANNNNNTGG 2 cut(s) 571, 763
PkrI GCNGC 2 cut(s) 301, 953
PleI GAGTC 6 cut(s) 194, 532, 780, 881, 1058, 1216
PmaCI CACGTG 1 cut(s) 474
PmlI CACGTG 1 cut(s) 474
PpsI GAGTC 6 cut(s) 194, 532, 780, 881, 1058, 1216
Ppu21I YACGTR 1 cut(s) 474
PpuMI RGGWCCY 1 cut(s) 1472
PshAI GACNNNNGTC 1 cut(s) 1470
PsiI TTATAA 1 cut(s) 177
Psp5II RGGWCCY 1 cut(s) 1472
PspCI CACGTG 1 cut(s) 474
PspEI GGTNACC 1 cut(s) 140
PspN4I GGNNCC 6 cut(s) 550, 755, 811, 1155, 1473, 1474
PspPI GGNCC 4 cut(s) 364, 917, 1139, 1472
PspPPI RGGWCCY 1 cut(s) 1472
PsuI RGATCY 1 cut(s) 1153
PsyI GACNNNGTC 1 cut(s) 971
RsaI GTAC 1 cut(s) 1507
RsaNI GTAC 1 cut(s) 1506
RseI CAYNNNNRTG 1 cut(s) 472
SaqAI TTAA 4 cut(s) 111, 906, 1266, 1368
SatI GCNGC 2 cut(s) 300, 952
Sau3AI GATC 2 cut(s) 1153, 1374
Sau96I GGNCC 4 cut(s) 364, 917, 1139, 1472
SchI GAGTC 6 cut(s) 195, 533, 781, 881, 1058, 1217
ScrFI CCNGG 3 cut(s) 159, 160, 367
SduI GDGCHC 3 cut(s) 519, 1084, 1408
SfaNI GCATC 1 cut(s) 670
SinI GGWCC 2 cut(s) 917, 1472
SmaI CCCGGG 1 cut(s) 160
SmiMI CAYNNNNRTG 1 cut(s) 472
SmlI CTYRAG 2 cut(s) 25, 1193
SmoI CTYRAG 2 cut(s) 25, 1193
Sse9I AATT 4 cut(s) 49, 371, 739, 1050
SseBI AGGCCT 1 cut(s) 488
SsiI CCGC 2 cut(s) 297, 856
SspMI CTAG 2 cut(s) 939, 1463
StuI AGGCCT 1 cut(s) 488
StyD4I CCNGG 3 cut(s) 157, 158, 365
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 4 cut(s) 37, 146, 904, 981
TaiI ACGT 1 cut(s) 476
TaqI TCGA 1 cut(s) 788
TaqII GACCGA 1 cut(s) 93
TasI AATT 4 cut(s) 49, 371, 739, 1050
TatI WGTACW 1 cut(s) 1505
TfiI GAWTC 3 cut(s) 5, 460, 932
Tru1I TTAA 4 cut(s) 111, 906, 1266, 1368
Tru9I TTAA 4 cut(s) 111, 906, 1266, 1368
TscAI CASTG 2 cut(s) 432, 1493
TseFI GTSAC 3 cut(s) 575, 884, 1299
TseI GCWGC 2 cut(s) 299, 951
Tsp45I GTSAC 3 cut(s) 575, 884, 1299
TspDTI ATGAA 6 cut(s) 279, 609, 633, 1061, 1181, 1345
TspGWI ACGGA 2 cut(s) 536, 1092
TspMI CCCGGG 1 cut(s) 158
TspRI CASTG 2 cut(s) 432, 1493
Tth111I GACNNNGTC 1 cut(s) 971
Van91I CCANNNNNTGG 2 cut(s) 571, 763
VpaK11BI GGWCC 2 cut(s) 917, 1472
XapI RAATTY 2 cut(s) 739, 1050
XbaI TCTAGA 1 cut(s) 1462
XmaI CCCGGG 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 260, 899
XmnI GAANNNNTTC 4 cut(s) 15, 435, 877, 1348
XspI CTAG 2 cut(s) 939, 1463
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.