Rh3CG238600

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0007394
Physical Location & Seq
Reverse (-)
22832089 .. 22834100
2012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG238600.1

Sequence Viewer

Length: 1536 bp
ATGGAGTCGGAGGCACTTCCGTCCCCGTCGAGGAATGTGGCGAAATGTTGCAACTGCGAATGTAGTTGCACGTTGACGACTGGTTCCTCGGGGACATGGATTCGGTCGGTCAAGAGGAAATATGCCGAGTTGGAGGAGGAGGGTAACCGATTCTTTATACCCGGGTTGGAAGTCTATTACAATGCAAAAGTCCAACTGGATCACGAATGTAATGCACTGCGTGAGACTCTTAGTAGCCAGCAGAAAACAATACAAGATTTGTACACCGAACTGGATGAGGAGAGGAATGCTGCTTCATCGGCTGCAAATGAGGCTATGTCCATGATACTGAGGTTGCAGAGCGAGAAGGCTGAAATACAGATGGAGGCCAGGCAGTTCAAGCTCTTTGTCGAGGAAAAGATGGCACATGACCAGCAGGAGCTTCTGGCATTGGAAGATCTGTTGTATAAGAGAGAGCAGACAATTCAGGCACTCACCTGTGAAGTGCAGGCCTATAAACATCGGATGATGAGTTATGGGCTATCTGAGGCCGAGGCTGATGGTGCAGGGAGTGTGCTCAGCCGTAACCAGAGCATGGGGGATGTTGAATCCCAGTATGATCTCCCCACAACTCCCGTTTATGAATACCCACCTTTGAAATGCAAGATAAATGAGACCCAAGGTCCTTTGGAGGATGATGATGTAGCGGATATTGAGAAATATGCATTTGGTGAGACTCCTCGAGATCATTTGAAGAATTTAGAAAATAGGCTCTACGAGATGGAGAGGTGTCCGAGCAACACTCAGTTGGAAGGGGATTTTTCAGGTTCCAAGCACATATATGAAAAGGGGGTGATTGGCCATTCTCCTAGGCGGTCAAGACATTCCAGGATGGTTTCAAATGATTCGACTCCATATTCAGCAATTGTAAGAGAAGCCGGTGCAGATTTCTTTGCTGAATCTCCTAGGTATAGCAGCAGCTTCAAGAGGGCAGAGAATGTCTCCCACCCTGAGGACTACTCATATACGAGAAAGTTGGACAATGCATCAGAAATTGGAGACGACATGTGTGACAGAGTTTATACCATTGACTCTGTTCATAATGGAGCACCATACAGTGGTTTTACTGAGGCCAAAGCTGGAGCTGGTATCTATGGGGATTACGTAACTACACCAAGAGGGTCACTTCACCATGCCGAGGATCCTGATATCATGAAGCTCTATATGAGGCTTCAGGCACTTGAGGCTGACAGGGAGTCAATGAGGCAAACAATGCTTTCGATGCGAACTGATAAAGCTCAACTGGTGTTACTCAAAGAAATAGCTCAACACTTGTGTAAAGACATGTCACCAGATGGACGAGTGATTGTGAAGAAGCAATCTGGCTTTTCATTCATGTCAGTTTTTAAGTGGGTCAAGTCCTTTGTTTTCTGGAGAAGGAGAGCCCGTCGAAGCAAGTACATGTGTGGACTATCGGCCAATGTGGGTTTGCTGATGCTTTTAGAGAACAGCCCCCGTGCAAGGCAGTGGAGATGTCTTACTAGTACACAAGTCTGA

Protein Analysis

511

Amino Acids

58.17

Weight (kDa)

5.7

Isoelectric Point (pI)

52.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 72 - 162 9.5e-35 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 574, 1097
AciI CCGC 2 cut(s) 686, 853
AclWI GGATC 3 cut(s) 207, 1175, 1188
AcoI YGGCCR 2 cut(s) 838, 1455
AcsI RAATTY 1 cut(s) 736
AcuI CTGAAG 1 cut(s) 1196
AdeI CACNNNGTG 1 cut(s) 221
AfaI GTAC 3 cut(s) 263, 1439, 1525
AfiI CCNNNNNNNGG 6 cut(s) 30, 574, 991, 1097, 1177, 1500
AflIII ACRYGT 3 cut(s) 1044, 1323, 1440
AgsI TTSAA 6 cut(s) 379, 587, 637, 733, 879, 964
AhdI GACNNNNNGTC 2 cut(s) 660, 1234
AhlI ACTAGT 1 cut(s) 1520
AjnI CCWGG 2 cut(s) 368, 866
AluBI AGCT 8 cut(s) 382, 421, 960, 1118, 1124, 1198, 1277, 1304
AluI AGCT 8 cut(s) 382, 421, 960, 1118, 1124, 1198, 1277, 1304
Alw21I GWGCWC 2 cut(s) 558, 1090
Alw26I GTCTC 5 cut(s) 218, 647, 707, 985, 1032
AlwI GGATC 3 cut(s) 207, 1175, 1188
Ama87I CYCGRG 3 cut(s) 88, 161, 720
AoxI GGCC 6 cut(s) 366, 489, 528, 838, 1110, 1455
ApeKI GCWGC 4 cut(s) 290, 302, 954, 957
ApoI RAATTY 1 cut(s) 736
AspA2I CCTAGG 2 cut(s) 848, 944
AspS9I GGNCC 1 cut(s) 662
AsuC2I CCSGG 2 cut(s) 162, 163
AsuHPI GGTGA 5 cut(s) 466, 722, 844, 1160, 1320
AvaI CYCGRG 3 cut(s) 88, 161, 720
AvaII GGWCC 1 cut(s) 662
AvrII CCTAGG 2 cut(s) 848, 944
AxyI CCTNAGG 1 cut(s) 990
BaeI ACNNNNGTAYC 2 cut(s) 317, 350
BalI TGGCCA 1 cut(s) 840
BamHI GGATCC 1 cut(s) 1180
BanII GRGCYC 1 cut(s) 1426
Bbv12I GWGCWC 2 cut(s) 558, 1090
BbvI GCAGC 4 cut(s) 277, 289, 966, 969
BccI CCATC 6 cut(s) 355, 394, 533, 754, 865, 1328
BceAI ACGGC 1 cut(s) 546
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 2 cut(s) 370, 868
BcnI CCSGG 2 cut(s) 162, 163
BcoDI GTCTC 5 cut(s) 218, 647, 707, 985, 1032
BcuI ACTAGT 1 cut(s) 1520
BfaI CTAG 3 cut(s) 849, 945, 1521
BglII AGATCT 1 cut(s) 436
BisI GCNGC 4 cut(s) 291, 303, 955, 958
BlnI CCTAGG 2 cut(s) 848, 944
BlpI GCTNAGC 1 cut(s) 557
BlsI GCNGC 4 cut(s) 292, 304, 956, 959
Bme1390I CCNGG 4 cut(s) 162, 163, 370, 868
Bme18I GGWCC 1 cut(s) 662
BmeRI GACNNNNNGTC 2 cut(s) 660, 1234
BmeT110I CYCGRG 3 cut(s) 88, 161, 720
BmgT120I GGNCC 1 cut(s) 662
BmiI GGNNCC 3 cut(s) 85, 808, 1182
BmrFI CCNGG 4 cut(s) 162, 163, 370, 868
BmrI ACTGGG 1 cut(s) 586
BmsI GCATC 3 cut(s) 1034, 1251, 1464
BmuI ACTGGG 1 cut(s) 586
BplI GAGNNNNNCTC 6 cut(s) 766, 798, 965, 997, 983, 1015
BpmI CTGGAG 2 cut(s) 1140, 1432
Bpu1102I GCTNAGC 1 cut(s) 557
BpuEI CTTGAG 1 cut(s) 1241
BpuMI CCSGG 2 cut(s) 162, 163
BsaAI YACGTR 1 cut(s) 1144
BsaI GGTCTC 1 cut(s) 647
BsaJI CCNNGG 7 cut(s) 87, 161, 531, 658, 848, 944, 1176
BsaXI ACNNNNNCTCC 2 cut(s) 1411, 1441
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 574, 991, 1097, 1177, 1500
Bse118I RCCGGY 1 cut(s) 917
Bse1I ACTGG 5 cut(s) 85, 201, 276, 592, 1287
Bse21I CCTNAGG 1 cut(s) 990
BseBI CCWGG 2 cut(s) 370, 868
BseDI CCNNGG 7 cut(s) 87, 161, 531, 658, 848, 944, 1176
BseGI GGATG 5 cut(s) 280, 510, 586, 679, 876
BseLI CCNNNNNNNGG 6 cut(s) 30, 574, 991, 1097, 1177, 1500
BseMII CTCAG 6 cut(s) 320, 516, 571, 797, 981, 1098
BseNI ACTGG 5 cut(s) 85, 201, 276, 592, 1287
BseRI GAGGAG 4 cut(s) 149, 152, 293, 708
BseXI GCAGC 4 cut(s) 277, 289, 966, 969
BsgI GTGCAG 3 cut(s) 506, 564, 942
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 6 cut(s) 368, 491, 530, 840, 1112, 1457
BsiEI CGRYCG 1 cut(s) 107
BsiHKAI GWGCWC 2 cut(s) 558, 1090
BsiHKCI CYCGRG 3 cut(s) 88, 161, 720
BsiSI CCGG 2 cut(s) 162, 918
BslFI GGGAC 2 cut(s) 7, 106
BslI CCNNNNNNNGG 6 cut(s) 30, 574, 991, 1097, 1177, 1500
BsmAI GTCTC 5 cut(s) 218, 647, 707, 985, 1032
BsmBI CGTCTC 1 cut(s) 1032
BsmFI GGGAC 2 cut(s) 7, 106
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 6 cut(s) 368, 491, 530, 840, 1112, 1457
Bso31I GGTCTC 1 cut(s) 647
BsoBI CYCGRG 3 cut(s) 88, 161, 720
Bsp1286I GDGCHC 3 cut(s) 558, 1090, 1426
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 5 cut(s) 199, 436, 598, 724, 1180
Bsp1720I GCTNAGC 1 cut(s) 557
BspACI CCGC 2 cut(s) 686, 853
BspANI GGCC 6 cut(s) 368, 491, 530, 840, 1112, 1457
BspCNI CTCAG 6 cut(s) 321, 517, 570, 796, 982, 1099
BspHI TCATGA 1 cut(s) 1191
BspLI GGNNCC 3 cut(s) 85, 808, 1182
BspPI GGATC 3 cut(s) 207, 1175, 1188
BspTNI GGTCTC 1 cut(s) 647
BsrFI RCCGGY 1 cut(s) 917
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 5 cut(s) 85, 201, 276, 592, 1287
BssAI RCCGGY 1 cut(s) 917
BssECI CCNNGG 7 cut(s) 87, 161, 531, 658, 848, 944, 1176
BssMI GATC 5 cut(s) 199, 436, 598, 724, 1180
BssT1I CCWWGG 3 cut(s) 658, 848, 944
Bst2UI CCWGG 2 cut(s) 370, 868
Bst4CI ACNGT 1 cut(s) 1097
BstAPI GCANNNNNTGC 1 cut(s) 1252
BstAUI TGTACA 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 1144
BstC8I GCNNGC 2 cut(s) 239, 489
BstDEI CTNAG 7 cut(s) 230, 329, 525, 557, 783, 990, 1107
BstEII GGTNACC 1 cut(s) 143
BstF5I GGATG 5 cut(s) 280, 510, 586, 679, 876
BstKTI GATC 5 cut(s) 202, 439, 601, 727, 1183
BstMAI GTCTC 5 cut(s) 218, 647, 707, 985, 1032
BstMBI GATC 5 cut(s) 199, 436, 598, 724, 1180
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 7 cut(s) 299, 311, 379, 542, 1223, 1252, 1261
BstNI CCWGG 2 cut(s) 370, 868
BstNSI RCATGY 3 cut(s) 1048, 1327, 1444
BstPI GGTNACC 1 cut(s) 143
BstSCI CCNGG 4 cut(s) 160, 161, 368, 866
BstSNI TACGTA 1 cut(s) 1144
BstV1I GCAGC 4 cut(s) 277, 289, 966, 969
BstX2I RGATCY 2 cut(s) 436, 1180
BstYI RGATCY 2 cut(s) 436, 1180
Bsu36I CCTNAGG 1 cut(s) 990
BsuRI GGCC 6 cut(s) 368, 491, 530, 840, 1112, 1457
BtsCI GGATG 5 cut(s) 280, 510, 586, 679, 876
BtsI GCAGTG 2 cut(s) 215, 1511
BtsIMutI CAGTG 3 cut(s) 215, 1102, 1511
Cac8I GCNNGC 2 cut(s) 239, 489
CciI TCATGA 1 cut(s) 1191
Cfr10I RCCGGY 1 cut(s) 917
Cfr13I GGNCC 1 cut(s) 662
Cfr9I CCCGGG 1 cut(s) 161
Csp6I GTAC 3 cut(s) 262, 1438, 1524
CviQI GTAC 3 cut(s) 262, 1438, 1524
DdeI CTNAG 7 cut(s) 230, 329, 525, 557, 783, 990, 1107
DpnI GATC 5 cut(s) 201, 438, 600, 726, 1182
DpnII GATC 5 cut(s) 199, 436, 598, 724, 1180
DraIII CACNNNGTG 1 cut(s) 221
DriI GACNNNNNGTC 2 cut(s) 660, 1234
EaeI YGGCCR 2 cut(s) 838, 1455
Eam1105I GACNNNNNGTC 2 cut(s) 660, 1234
Eco105I TACGTA 1 cut(s) 1144
Eco130I CCWWGG 3 cut(s) 658, 848, 944
Eco147I AGGCCT 1 cut(s) 491
Eco24I GRGCYC 1 cut(s) 1426
Eco31I GGTCTC 1 cut(s) 647
Eco32I GATATC 1 cut(s) 1189
Eco47I GGWCC 1 cut(s) 662
Eco57I CTGAAG 1 cut(s) 1196
Eco81I CCTNAGG 1 cut(s) 990
Eco88I CYCGRG 3 cut(s) 88, 161, 720
Eco91I GGTNACC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 368, 866
EcoRV GATATC 1 cut(s) 1189
EcoT14I CCWWGG 3 cut(s) 658, 848, 944
EcoT22I ATGCAT 2 cut(s) 706, 1027
EcoT38I GRGCYC 1 cut(s) 1426
ErhI CCWWGG 3 cut(s) 658, 848, 944
Esp3I CGTCTC 1 cut(s) 1032
FaqI GGGAC 2 cut(s) 7, 106
Fnu4HI GCNGC 4 cut(s) 291, 303, 955, 958
FokI GGATG 5 cut(s) 287, 517, 593, 686, 883
FriOI GRGCYC 1 cut(s) 1426
Fsp4HI GCNGC 4 cut(s) 291, 303, 955, 958
FspBI CTAG 3 cut(s) 849, 945, 1521
GluI GCNGC 4 cut(s) 291, 303, 955, 958
GsuI CTGGAG 2 cut(s) 1140, 1432
HaeIII GGCC 6 cut(s) 368, 491, 530, 840, 1112, 1457
HapII CCGG 2 cut(s) 162, 918
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HpaII CCGG 2 cut(s) 162, 918
HphI GGTGA 5 cut(s) 466, 722, 844, 1160, 1320
Hpy166II GTNNAC 4 cut(s) 75, 264, 1448, 1526
Hpy188I TCNGA 6 cut(s) 10, 504, 526, 774, 1030, 1535
Hpy188III TCNNGA 8 cut(s) 112, 203, 722, 858, 964, 1184, 1192, 1411
Hpy8I GTNNAC 4 cut(s) 75, 264, 1448, 1526
Hpy99I CGWCG 2 cut(s) 31, 1431
HpyAV CCTTC 3 cut(s) 340, 785, 1410
HpyCH4III ACNGT 1 cut(s) 1097
HpyCH4IV ACGT 2 cut(s) 71, 1143
HpyF10VI GCNNNNNNNGC 7 cut(s) 299, 311, 379, 542, 1223, 1252, 1261
HpyF3I CTNAG 7 cut(s) 230, 329, 525, 557, 783, 990, 1107
HpySE526I ACGT 2 cut(s) 71, 1143
Kzo9I GATC 5 cut(s) 199, 436, 598, 724, 1180
LmnI GCTCC 3 cut(s) 418, 1085, 1121
Lsp1109I GCAGC 4 cut(s) 277, 289, 966, 969
LweI GCATC 3 cut(s) 1034, 1251, 1464
MaeI CTAG 3 cut(s) 849, 945, 1521
MaeII ACGT 2 cut(s) 71, 1143
MaeIII GTNAC 7 cut(s) 143, 563, 1049, 1144, 1161, 1287, 1326
MalI GATC 5 cut(s) 201, 438, 600, 726, 1182
MboI GATC 5 cut(s) 199, 436, 598, 724, 1180
MboII GAAGA 3 cut(s) 446, 745, 1363
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 2 cut(s) 436, 1180
MhlI GDGCHC 3 cut(s) 558, 1090, 1426
MlsI TGGCCA 1 cut(s) 840
MluCI AATT 4 cut(s) 462, 736, 903, 1032
MluNI TGGCCA 1 cut(s) 840
MlyI GAGTC 6 cut(s) 14, 220, 709, 883, 1064, 1244
MmeI TCCRAC 5 cut(s) 111, 147, 217, 768, 996
Mox20I TGGCCA 1 cut(s) 840
Mph1103I ATGCAT 2 cut(s) 706, 1027
MscI TGGCCA 1 cut(s) 840
MseI TTAA 1 cut(s) 1386
MslI CAYNNNNRTG 1 cut(s) 819
Msp20I TGGCCA 1 cut(s) 840
MspI CCGG 2 cut(s) 162, 918
MspR9I CCNGG 4 cut(s) 162, 163, 370, 868
MunI CAATTG 1 cut(s) 903
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 2 cut(s) 370, 868
MwoI GCNNNNNNNGC 7 cut(s) 299, 311, 379, 542, 1223, 1252, 1261
NciI CCSGG 2 cut(s) 162, 163
NdeII GATC 5 cut(s) 199, 436, 598, 724, 1180
NlaIV GGNNCC 3 cut(s) 85, 808, 1182
NmeAIII GCCGAG 3 cut(s) 151, 556, 1201
NmuCI GTSAC 3 cut(s) 1049, 1161, 1326
NsiI ATGCAT 2 cut(s) 706, 1027
NspI RCATGY 3 cut(s) 1048, 1327, 1444
PaeR7I CTCGAG 1 cut(s) 720
PagI TCATGA 1 cut(s) 1191
PceI AGGCCT 1 cut(s) 491
PciI ACATGT 3 cut(s) 1044, 1323, 1440
PctI GAATGC 1 cut(s) 292
PfeI GAWTC 5 cut(s) 100, 150, 587, 884, 938
PflMI CCANNNNNTGG 2 cut(s) 574, 1097
PfoI TCCNGGA 1 cut(s) 866
PkrI GCNGC 4 cut(s) 292, 304, 956, 959
PleI GAGTC 6 cut(s) 13, 220, 709, 883, 1064, 1243
PpsI GAGTC 6 cut(s) 13, 220, 709, 883, 1064, 1243
Ppu21I YACGTR 1 cut(s) 1144
PpuMI RGGWCCY 1 cut(s) 662
PscI ACATGT 3 cut(s) 1044, 1323, 1440
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 2 cut(s) 368, 866
PspEI GGTNACC 1 cut(s) 143
PspGI CCWGG 2 cut(s) 368, 866
PspN4I GGNNCC 3 cut(s) 85, 808, 1182
PspPI GGNCC 1 cut(s) 662
PspPPI RGGWCCY 1 cut(s) 662
PsuI RGATCY 2 cut(s) 436, 1180
RsaI GTAC 3 cut(s) 263, 1439, 1525
RsaNI GTAC 3 cut(s) 262, 1438, 1524
RseI CAYNNNNRTG 1 cut(s) 819
SaqAI TTAA 1 cut(s) 1386
SatI GCNGC 4 cut(s) 291, 303, 955, 958
Sau3AI GATC 5 cut(s) 199, 436, 598, 724, 1180
Sau96I GGNCC 1 cut(s) 662
SchI GAGTC 6 cut(s) 14, 220, 709, 883, 1064, 1244
ScrFI CCNGG 4 cut(s) 162, 163, 370, 868
SduI GDGCHC 3 cut(s) 558, 1090, 1426
SfaNI GCATC 3 cut(s) 1034, 1251, 1464
Sfr274I CTCGAG 1 cut(s) 720
SinI GGWCC 1 cut(s) 662
SlaI CTCGAG 1 cut(s) 720
SmaI CCCGGG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 2 cut(s) 720, 1220
SmoI CTYRAG 2 cut(s) 720, 1220
SnaBI TACGTA 1 cut(s) 1144
SpeI ACTAGT 1 cut(s) 1520
Sse9I AATT 4 cut(s) 462, 736, 903, 1032
SseBI AGGCCT 1 cut(s) 491
SsiI CCGC 2 cut(s) 686, 853
SspMI CTAG 3 cut(s) 849, 945, 1521
StuI AGGCCT 1 cut(s) 491
StyD4I CCNGG 4 cut(s) 160, 161, 368, 866
StyI CCWWGG 3 cut(s) 658, 848, 944
TaaI ACNGT 1 cut(s) 1097
TaiI ACGT 2 cut(s) 74, 1146
TaqI TCGA 6 cut(s) 29, 390, 721, 887, 1259, 1429
TaqII GACCGA 2 cut(s) 93, 97
TasI AATT 4 cut(s) 462, 736, 903, 1032
TatI WGTACW 3 cut(s) 261, 1437, 1523
TfiI GAWTC 5 cut(s) 100, 150, 587, 884, 938
Tru1I TTAA 1 cut(s) 1386
Tru9I TTAA 1 cut(s) 1386
TscAI CASTG 3 cut(s) 222, 1102, 1511
TseFI GTSAC 3 cut(s) 1049, 1161, 1326
TseI GCWGC 4 cut(s) 290, 302, 954, 957
Tsp45I GTSAC 3 cut(s) 1049, 1161, 1326
TspDTI ATGAA 7 cut(s) 285, 636, 837, 1067, 1208, 1359, 1363
TspGWI ACGGA 1 cut(s) 9
TspMI CCCGGG 1 cut(s) 161
TspRI CASTG 3 cut(s) 222, 1102, 1511
Van91I CCANNNNNTGG 2 cut(s) 574, 1097
VpaK11BI GGWCC 1 cut(s) 662
XapI RAATTY 1 cut(s) 736
XceI RCATGY 3 cut(s) 1048, 1327, 1444
XhoI CTCGAG 1 cut(s) 720
XmaI CCCGGG 1 cut(s) 161
XmaJI CCTAGG 2 cut(s) 848, 944
XspI CTAG 3 cut(s) 849, 945, 1521
Zsp2I ATGCAT 2 cut(s) 706, 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.