Rh3AG211300

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0007394
Physical Location & Seq
Reverse (-)
21268163 .. 21272209
4047 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG211300.1

Sequence Viewer

Length: 1440 bp
ATGGAGTCGGACGCACTTCCGTCCCCGTCGAGGAATGTGGCGAAATGTTGCAATTGCGAATGTAGTTGCACGTTGACGACTGGTTCCTCGGGGACATGGATTCGGTCGGTCAAGAGGAAATATGCCGAGTTGGAGGAGGAGGGTAACCGATTCTTTATACCCGGGTTGGAAGTCTATTACAATGCAAAAGTCCAACTGGATCACGAATGTAATGCACTGCGTGAGACTCTTAGTAGCCAGCAGAAAACAATACAAGATTTGTACACCGAACTGGATGAGGAGAGGAATGCTGCTTCATCGGCTGCAAATGAGGCTATGTCCATGATACTGAGGTTGCAGAGCGAGAAGGCTGAAATACAGATGGAGGCCAGGCAGTTCAAGCTCTTTGTCGAGGAAAAGATGGCACATGACCAGCAGGAGCTTCTGGCATTGGAAGATCTATTGTATAAGAGAGAGCAGACAATTCAGGCACTCACCTGTGAAGTGCAGGCCTATAAACATCGGATGATGAGTTATGGGCTATCTGAGGCCGAGGCTGATGGTGCAGGGAGTGTGCTCAGCCGTAACCAGAGCATGGGGGATGTTGAATCCCAGTATGATCTCCCCACAACTCCCGTTTATGAATACCCACCTTTGAAATGCAAGATAAATGAGACCCAAGGTCCTTTGGAGGATGATGATGTAGCGGATATTGAGAAATATGCATTTGGTGAGACTCCTCGAGATCATTTGAAGAATTTAGAAAATAGGCTCTACCAGATGGAGAGGTGTCCGAGCAACACTCAGTTGGAAGGGGATTTTTCAGGTTCCAAGCACATATATGAAAAGGGGGTGATTGGCCATTCTCCTAGGCGGTCAAGACATTCCAGGATGGTTTCAAATGATTCGACTCCATATTCAGCAATTGTAAGAGAAGCCGGTGCAGATTTCTTTGCTGAATCTCCTAGGTATAGCAGCAGCTTCAAGAGGGCAGAGAATGTCTCCCACCCTGAGGACTACTCATATACGAGAAAGTTGGACAATGCATCAGAAATTGGAGACGACATGTGTGACAGAGTTTATACCATTGACTCTGTTCATAATGGAGCACCATACAGTGGTTTTACTGAGGCCAAAGCTGGAGCTGGGATCTATGGGGATTACGTAACTACACCAAGAGGGTCACTTCACCATGCCGAGGATCCTGACATCATGAAGCTCTATATGAGGCTTCAGGCACTTGAGGCTGACAGGGAGTCAATGAGGCAAACAATGCTTTCGATGCGAACTGATAAAGCTCAACTGGTGTTACTCAAAGAAATAGCTCAACACTTGTGTAAAGACATGTCACCAGATGGACGAGTGATTGTGAAGAAGCAATCTGGCTTTTCATTCATGTCAGTTTTTAAGTGGGTCAAGTCCTTTGTTTTCTGGAGAAGGAGAGCCCGTCGAAGCAAGTAA

Protein Analysis

479

Amino Acids

54.56

Weight (kDa)

5.61

Isoelectric Point (pI)

52.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 72 - 162 8.5e-35 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 574, 1097
AciI CCGC 2 cut(s) 686, 853
AclWI GGATC 4 cut(s) 207, 1136, 1175, 1188
AcoI YGGCCR 1 cut(s) 838
AcsI RAATTY 1 cut(s) 736
AcuI CTGAAG 1 cut(s) 1196
AdeI CACNNNGTG 1 cut(s) 221
AfaI GTAC 1 cut(s) 263
AfiI CCNNNNNNNGG 5 cut(s) 30, 574, 991, 1097, 1177
AflIII ACRYGT 2 cut(s) 1044, 1323
AgsI TTSAA 6 cut(s) 379, 587, 637, 733, 879, 964
AhdI GACNNNNNGTC 2 cut(s) 660, 1234
AjnI CCWGG 2 cut(s) 368, 866
AluBI AGCT 8 cut(s) 382, 421, 960, 1118, 1124, 1198, 1277, 1304
AluI AGCT 8 cut(s) 382, 421, 960, 1118, 1124, 1198, 1277, 1304
Alw21I GWGCWC 2 cut(s) 558, 1090
Alw26I GTCTC 5 cut(s) 218, 647, 707, 985, 1032
AlwI GGATC 4 cut(s) 207, 1136, 1175, 1188
Ama87I CYCGRG 3 cut(s) 88, 161, 720
AoxI GGCC 5 cut(s) 366, 489, 528, 838, 1110
ApeKI GCWGC 4 cut(s) 290, 302, 954, 957
ApoI RAATTY 1 cut(s) 736
AspA2I CCTAGG 2 cut(s) 848, 944
AspS9I GGNCC 1 cut(s) 662
AsuC2I CCSGG 2 cut(s) 162, 163
AsuHPI GGTGA 5 cut(s) 466, 722, 844, 1160, 1320
AvaI CYCGRG 3 cut(s) 88, 161, 720
AvaII GGWCC 1 cut(s) 662
AvrII CCTAGG 2 cut(s) 848, 944
AxyI CCTNAGG 1 cut(s) 990
BaeI ACNNNNGTAYC 2 cut(s) 317, 350
BalI TGGCCA 1 cut(s) 840
BamHI GGATCC 1 cut(s) 1180
BanII GRGCYC 1 cut(s) 1426
Bbv12I GWGCWC 2 cut(s) 558, 1090
BbvI GCAGC 4 cut(s) 277, 289, 966, 969
BccI CCATC 6 cut(s) 355, 394, 533, 754, 865, 1328
BceAI ACGGC 1 cut(s) 546
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 2 cut(s) 370, 868
BcnI CCSGG 2 cut(s) 162, 163
BcoDI GTCTC 5 cut(s) 218, 647, 707, 985, 1032
BfaI CTAG 2 cut(s) 849, 945
BglII AGATCT 1 cut(s) 436
BisI GCNGC 4 cut(s) 291, 303, 955, 958
BlnI CCTAGG 2 cut(s) 848, 944
BlpI GCTNAGC 1 cut(s) 557
BlsI GCNGC 4 cut(s) 292, 304, 956, 959
Bme1390I CCNGG 4 cut(s) 162, 163, 370, 868
Bme18I GGWCC 1 cut(s) 662
BmeRI GACNNNNNGTC 2 cut(s) 660, 1234
BmeT110I CYCGRG 3 cut(s) 88, 161, 720
BmgT120I GGNCC 1 cut(s) 662
BmiI GGNNCC 3 cut(s) 85, 808, 1182
BmrFI CCNGG 4 cut(s) 162, 163, 370, 868
BmrI ACTGGG 1 cut(s) 586
BmsI GCATC 2 cut(s) 1034, 1251
BmuI ACTGGG 1 cut(s) 586
BplI GAGNNNNNCTC 6 cut(s) 766, 798, 965, 997, 983, 1015
BpmI CTGGAG 2 cut(s) 1140, 1432
Bpu1102I GCTNAGC 1 cut(s) 557
BpuEI CTTGAG 1 cut(s) 1241
BpuMI CCSGG 2 cut(s) 162, 163
BsaAI YACGTR 1 cut(s) 1144
BsaI GGTCTC 1 cut(s) 647
BsaJI CCNNGG 7 cut(s) 87, 161, 531, 658, 848, 944, 1176
BsaXI ACNNNNNCTCC 1 cut(s) 1411
Bsc4I CCNNNNNNNGG 5 cut(s) 30, 574, 991, 1097, 1177
Bse118I RCCGGY 1 cut(s) 917
Bse1I ACTGG 5 cut(s) 85, 201, 276, 592, 1287
Bse21I CCTNAGG 1 cut(s) 990
BseBI CCWGG 2 cut(s) 370, 868
BseDI CCNNGG 7 cut(s) 87, 161, 531, 658, 848, 944, 1176
BseGI GGATG 5 cut(s) 280, 510, 586, 679, 876
BseLI CCNNNNNNNGG 5 cut(s) 30, 574, 991, 1097, 1177
BseMII CTCAG 6 cut(s) 320, 516, 571, 797, 981, 1098
BseNI ACTGG 5 cut(s) 85, 201, 276, 592, 1287
BseRI GAGGAG 4 cut(s) 149, 152, 293, 708
BseXI GCAGC 4 cut(s) 277, 289, 966, 969
BseYI CCCAGC 1 cut(s) 1124
BsgI GTGCAG 3 cut(s) 506, 564, 942
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 5 cut(s) 368, 491, 530, 840, 1112
BsiEI CGRYCG 1 cut(s) 107
BsiHKAI GWGCWC 2 cut(s) 558, 1090
BsiHKCI CYCGRG 3 cut(s) 88, 161, 720
BsiSI CCGG 2 cut(s) 162, 918
BslFI GGGAC 2 cut(s) 7, 106
BslI CCNNNNNNNGG 5 cut(s) 30, 574, 991, 1097, 1177
BsmAI GTCTC 5 cut(s) 218, 647, 707, 985, 1032
BsmBI CGTCTC 1 cut(s) 1032
BsmFI GGGAC 2 cut(s) 7, 106
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 5 cut(s) 368, 491, 530, 840, 1112
Bso31I GGTCTC 1 cut(s) 647
BsoBI CYCGRG 3 cut(s) 88, 161, 720
Bsp1286I GDGCHC 3 cut(s) 558, 1090, 1426
Bsp1407I TGTACA 1 cut(s) 261
Bsp143I GATC 6 cut(s) 199, 436, 598, 724, 1128, 1180
Bsp1720I GCTNAGC 1 cut(s) 557
BspACI CCGC 2 cut(s) 686, 853
BspANI GGCC 5 cut(s) 368, 491, 530, 840, 1112
BspCNI CTCAG 6 cut(s) 321, 517, 570, 796, 982, 1099
BspHI TCATGA 1 cut(s) 1191
BspLI GGNNCC 3 cut(s) 85, 808, 1182
BspPI GGATC 4 cut(s) 207, 1136, 1175, 1188
BspTNI GGTCTC 1 cut(s) 647
BsrFI RCCGGY 1 cut(s) 917
BsrGI TGTACA 1 cut(s) 261
BsrI ACTGG 5 cut(s) 85, 201, 276, 592, 1287
BssAI RCCGGY 1 cut(s) 917
BssECI CCNNGG 7 cut(s) 87, 161, 531, 658, 848, 944, 1176
BssMI GATC 6 cut(s) 199, 436, 598, 724, 1128, 1180
BssT1I CCWWGG 3 cut(s) 658, 848, 944
Bst2UI CCWGG 2 cut(s) 370, 868
Bst4CI ACNGT 1 cut(s) 1097
BstAPI GCANNNNNTGC 1 cut(s) 1252
BstAUI TGTACA 1 cut(s) 261
BstBAI YACGTR 1 cut(s) 1144
BstC8I GCNNGC 2 cut(s) 239, 489
BstDEI CTNAG 7 cut(s) 230, 329, 525, 557, 783, 990, 1107
BstEII GGTNACC 1 cut(s) 143
BstF5I GGATG 5 cut(s) 280, 510, 586, 679, 876
BstKTI GATC 6 cut(s) 202, 439, 601, 727, 1131, 1183
BstMAI GTCTC 5 cut(s) 218, 647, 707, 985, 1032
BstMBI GATC 6 cut(s) 199, 436, 598, 724, 1128, 1180
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 7 cut(s) 299, 311, 379, 542, 1223, 1252, 1261
BstNI CCWGG 2 cut(s) 370, 868
BstNSI RCATGY 2 cut(s) 1048, 1327
BstPI GGTNACC 1 cut(s) 143
BstSCI CCNGG 4 cut(s) 160, 161, 368, 866
BstSNI TACGTA 1 cut(s) 1144
BstV1I GCAGC 4 cut(s) 277, 289, 966, 969
BstX2I RGATCY 3 cut(s) 436, 1128, 1180
BstYI RGATCY 3 cut(s) 436, 1128, 1180
Bsu36I CCTNAGG 1 cut(s) 990
BsuRI GGCC 5 cut(s) 368, 491, 530, 840, 1112
BtsCI GGATG 5 cut(s) 280, 510, 586, 679, 876
BtsI GCAGTG 1 cut(s) 215
BtsIMutI CAGTG 2 cut(s) 215, 1102
Cac8I GCNNGC 2 cut(s) 239, 489
CciI TCATGA 1 cut(s) 1191
Cfr10I RCCGGY 1 cut(s) 917
Cfr13I GGNCC 1 cut(s) 662
Cfr9I CCCGGG 1 cut(s) 161
CseI GACGC 1 cut(s) 20
Csp6I GTAC 1 cut(s) 262
CviAII CATG 9 cut(s) 96, 322, 407, 574, 1045, 1172, 1192, 1324, 1375
CviQI GTAC 1 cut(s) 262
DdeI CTNAG 7 cut(s) 230, 329, 525, 557, 783, 990, 1107
DpnI GATC 6 cut(s) 201, 438, 600, 726, 1130, 1182
DpnII GATC 6 cut(s) 199, 436, 598, 724, 1128, 1180
DraIII CACNNNGTG 1 cut(s) 221
DriI GACNNNNNGTC 2 cut(s) 660, 1234
EaeI YGGCCR 1 cut(s) 838
Eam1105I GACNNNNNGTC 2 cut(s) 660, 1234
Eco105I TACGTA 1 cut(s) 1144
Eco130I CCWWGG 3 cut(s) 658, 848, 944
Eco147I AGGCCT 1 cut(s) 491
Eco24I GRGCYC 1 cut(s) 1426
Eco31I GGTCTC 1 cut(s) 647
Eco47I GGWCC 1 cut(s) 662
Eco57I CTGAAG 1 cut(s) 1196
Eco81I CCTNAGG 1 cut(s) 990
Eco88I CYCGRG 3 cut(s) 88, 161, 720
Eco91I GGTNACC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 662
EcoO65I GGTNACC 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 368, 866
EcoT14I CCWWGG 3 cut(s) 658, 848, 944
EcoT22I ATGCAT 2 cut(s) 706, 1027
EcoT38I GRGCYC 1 cut(s) 1426
ErhI CCWWGG 3 cut(s) 658, 848, 944
Esp3I CGTCTC 1 cut(s) 1032
FaeI CATG 9 cut(s) 99, 325, 410, 577, 1048, 1175, 1195, 1327, 1378
FaqI GGGAC 2 cut(s) 7, 106
FatI CATG 9 cut(s) 95, 321, 406, 573, 1044, 1171, 1191, 1323, 1374
Fnu4HI GCNGC 4 cut(s) 291, 303, 955, 958
FokI GGATG 5 cut(s) 287, 517, 593, 686, 883
FriOI GRGCYC 1 cut(s) 1426
Fsp4HI GCNGC 4 cut(s) 291, 303, 955, 958
FspBI CTAG 2 cut(s) 849, 945
GluI GCNGC 4 cut(s) 291, 303, 955, 958
GsaI CCCAGC 1 cut(s) 1128
GsuI CTGGAG 2 cut(s) 1140, 1432
HaeIII GGCC 5 cut(s) 368, 491, 530, 840, 1112
HapII CCGG 2 cut(s) 162, 918
HgaI GACGC 1 cut(s) 20
Hin1II CATG 9 cut(s) 99, 325, 410, 577, 1048, 1175, 1195, 1327, 1378
HincII GTYRAC 1 cut(s) 75
HindII GTYRAC 1 cut(s) 75
HpaII CCGG 2 cut(s) 162, 918
HphI GGTGA 5 cut(s) 466, 722, 844, 1160, 1320
Hpy166II GTNNAC 2 cut(s) 75, 264
Hpy188I TCNGA 5 cut(s) 10, 504, 526, 774, 1030
Hpy188III TCNNGA 8 cut(s) 112, 203, 722, 858, 964, 1184, 1192, 1411
Hpy8I GTNNAC 2 cut(s) 75, 264
Hpy99I CGWCG 2 cut(s) 31, 1431
HpyAV CCTTC 3 cut(s) 340, 785, 1410
HpyCH4III ACNGT 1 cut(s) 1097
HpyCH4IV ACGT 2 cut(s) 71, 1143
HpyF10VI GCNNNNNNNGC 7 cut(s) 299, 311, 379, 542, 1223, 1252, 1261
HpyF3I CTNAG 7 cut(s) 230, 329, 525, 557, 783, 990, 1107
HpySE526I ACGT 2 cut(s) 71, 1143
Hsp92II CATG 9 cut(s) 99, 325, 410, 577, 1048, 1175, 1195, 1327, 1378
Kzo9I GATC 6 cut(s) 199, 436, 598, 724, 1128, 1180
LmnI GCTCC 3 cut(s) 418, 1085, 1121
Lsp1109I GCAGC 4 cut(s) 277, 289, 966, 969
LweI GCATC 2 cut(s) 1034, 1251
MaeI CTAG 2 cut(s) 849, 945
MaeII ACGT 2 cut(s) 71, 1143
MaeIII GTNAC 7 cut(s) 143, 563, 1049, 1144, 1161, 1287, 1326
MalI GATC 6 cut(s) 201, 438, 600, 726, 1130, 1182
MboI GATC 6 cut(s) 199, 436, 598, 724, 1128, 1180
MboII GAAGA 3 cut(s) 446, 745, 1363
MfeI CAATTG 2 cut(s) 52, 903
MflI RGATCY 3 cut(s) 436, 1128, 1180
MhlI GDGCHC 3 cut(s) 558, 1090, 1426
MlsI TGGCCA 1 cut(s) 840
MluCI AATT 5 cut(s) 52, 462, 736, 903, 1032
MluNI TGGCCA 1 cut(s) 840
MlyI GAGTC 6 cut(s) 14, 220, 709, 883, 1064, 1244
MmeI TCCRAC 5 cut(s) 111, 147, 217, 768, 996
Mox20I TGGCCA 1 cut(s) 840
Mph1103I ATGCAT 2 cut(s) 706, 1027
MscI TGGCCA 1 cut(s) 840
MseI TTAA 1 cut(s) 1386
MslI CAYNNNNRTG 1 cut(s) 819
Msp20I TGGCCA 1 cut(s) 840
MspI CCGG 2 cut(s) 162, 918
MspR9I CCNGG 4 cut(s) 162, 163, 370, 868
MunI CAATTG 2 cut(s) 52, 903
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 2 cut(s) 370, 868
MwoI GCNNNNNNNGC 7 cut(s) 299, 311, 379, 542, 1223, 1252, 1261
NciI CCSGG 2 cut(s) 162, 163
NdeII GATC 6 cut(s) 199, 436, 598, 724, 1128, 1180
NlaIII CATG 9 cut(s) 99, 325, 410, 577, 1048, 1175, 1195, 1327, 1378
NlaIV GGNNCC 3 cut(s) 85, 808, 1182
NmeAIII GCCGAG 3 cut(s) 151, 556, 1201
NmuCI GTSAC 3 cut(s) 1049, 1161, 1326
NsiI ATGCAT 2 cut(s) 706, 1027
NspI RCATGY 2 cut(s) 1048, 1327
PaeR7I CTCGAG 1 cut(s) 720
PagI TCATGA 1 cut(s) 1191
PceI AGGCCT 1 cut(s) 491
PciI ACATGT 2 cut(s) 1044, 1323
PctI GAATGC 1 cut(s) 292
PfeI GAWTC 5 cut(s) 100, 150, 587, 884, 938
PflMI CCANNNNNTGG 2 cut(s) 574, 1097
PfoI TCCNGGA 1 cut(s) 866
PkrI GCNGC 4 cut(s) 292, 304, 956, 959
PleI GAGTC 6 cut(s) 13, 220, 709, 883, 1064, 1243
PpsI GAGTC 6 cut(s) 13, 220, 709, 883, 1064, 1243
Ppu21I YACGTR 1 cut(s) 1144
PpuMI RGGWCCY 1 cut(s) 662
PscI ACATGT 2 cut(s) 1044, 1323
Psp5II RGGWCCY 1 cut(s) 662
Psp6I CCWGG 2 cut(s) 368, 866
PspEI GGTNACC 1 cut(s) 143
PspFI CCCAGC 1 cut(s) 1124
PspGI CCWGG 2 cut(s) 368, 866
PspN4I GGNNCC 3 cut(s) 85, 808, 1182
PspPI GGNCC 1 cut(s) 662
PspPPI RGGWCCY 1 cut(s) 662
PsuI RGATCY 3 cut(s) 436, 1128, 1180
RsaI GTAC 1 cut(s) 263
RsaNI GTAC 1 cut(s) 262
RseI CAYNNNNRTG 1 cut(s) 819
SaqAI TTAA 1 cut(s) 1386
SatI GCNGC 4 cut(s) 291, 303, 955, 958
Sau3AI GATC 6 cut(s) 199, 436, 598, 724, 1128, 1180
Sau96I GGNCC 1 cut(s) 662
SchI GAGTC 6 cut(s) 14, 220, 709, 883, 1064, 1244
ScrFI CCNGG 4 cut(s) 162, 163, 370, 868
SduI GDGCHC 3 cut(s) 558, 1090, 1426
SfaNI GCATC 2 cut(s) 1034, 1251
Sfr274I CTCGAG 1 cut(s) 720
SinI GGWCC 1 cut(s) 662
SlaI CTCGAG 1 cut(s) 720
SmaI CCCGGG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 2 cut(s) 720, 1220
SmoI CTYRAG 2 cut(s) 720, 1220
SnaBI TACGTA 1 cut(s) 1144
Sse9I AATT 5 cut(s) 52, 462, 736, 903, 1032
SseBI AGGCCT 1 cut(s) 491
SsiI CCGC 2 cut(s) 686, 853
SspMI CTAG 2 cut(s) 849, 945
StuI AGGCCT 1 cut(s) 491
StyD4I CCNGG 4 cut(s) 160, 161, 368, 866
StyI CCWWGG 3 cut(s) 658, 848, 944
TaaI ACNGT 1 cut(s) 1097
TaiI ACGT 2 cut(s) 74, 1146
TaqI TCGA 6 cut(s) 29, 390, 721, 887, 1259, 1429
TaqII GACCGA 2 cut(s) 93, 97
TasI AATT 5 cut(s) 52, 462, 736, 903, 1032
TatI WGTACW 1 cut(s) 261
TfiI GAWTC 5 cut(s) 100, 150, 587, 884, 938
Tru1I TTAA 1 cut(s) 1386
Tru9I TTAA 1 cut(s) 1386
TscAI CASTG 2 cut(s) 222, 1102
TseFI GTSAC 3 cut(s) 1049, 1161, 1326
TseI GCWGC 4 cut(s) 290, 302, 954, 957
Tsp45I GTSAC 3 cut(s) 1049, 1161, 1326
TspDTI ATGAA 7 cut(s) 285, 636, 837, 1067, 1208, 1359, 1363
TspGWI ACGGA 1 cut(s) 9
TspMI CCCGGG 1 cut(s) 161
TspRI CASTG 2 cut(s) 222, 1102
Van91I CCANNNNNTGG 2 cut(s) 574, 1097
VpaK11BI GGWCC 1 cut(s) 662
XapI RAATTY 1 cut(s) 736
XceI RCATGY 2 cut(s) 1048, 1327
XhoI CTCGAG 1 cut(s) 720
XmaI CCCGGG 1 cut(s) 161
XmaJI CCTAGG 2 cut(s) 848, 944
XspI CTAG 2 cut(s) 849, 945
Zsp2I ATGCAT 2 cut(s) 706, 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.