pycom12g03990

Zein-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007394
Physical Location & Seq
Reverse (-)
3878808 .. 3879971
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g03990.1

Sequence Viewer

Length: 1164 bp
ATGGATTCAGAAGCATTTCCATCCTCAAGGCCGACGGTGAAATGTTGTAATTGTGAATGTAGTTGTTCGTTGGTGAGTGTTTCTTCAGGGGGGACTTGGCTTCGGTCTGTAAAGAGAAAGTATGGCGAGTTTGAGGATGGTAACCGTTTCTTTATACCCGGGATTGAACTTTATTATAATGCACGAGTCCAAATGGAGAACGAATGTGTTGCACTACGCGAGACGCTTAGTAGCCAGCAAAACACGATACAAGATTTGTATACTGAGCTGGACGAGGAGAGAAATGCTTCATCATCCGCTGCCAATGAGGCTATGTCAATGATATTGAGGTTGCAGGGGGAGAAGGCTGAAATACAGATGGAGGCCCGGCAATTCAAGTGTTTCGTTGAGGAGAAGATGGCACATGACCAGCAGGAGCTTTTGGCACTGGAAGATTTGCTGTATAAGAGAGAACAGGCGATTCAGGCACTCACGTGTGAAGTGCAGGCCTATAAGCATAGAATGATGAGTTATGGGCTCACGGAGTCTGAGGCAGAGGGTGAGAATGGTTCCTTTAGCCGTATCCAGAGTGTGGGTGACTTTGAAGCCCAGTATGAACTTCCGACAACGCCCACATATGAATACCCACCTTTGAAATGTAAAATAAATGAGACGCAAGGTGCATTGGAGTGCGATGATGATGCAGATATTGAGAAATATGCATTTGATGAGACCCCTCGTGGGAGAGAGCATTTGAGAAATTTGGAAAGCAGGCTCCATCAGATGGAGAGGAGTCCGGGCGGCAGTCCGATGGGAGGAGATTTTTCAGGTTCCAAGAACATTTTTGAAAAGGTGGTGGTTGGCCATTCTCCTCGGCGGTCAAGACATTCCGGAAGACATTCAAGTGACTCACCTGTGTATACGGTTAAAGAAACTGGTCCAGATTTTGCTGAATCTCCTAGATATAACAGCAGCTTCAGGAAGGCAGACTATGTCTCACAGTCTGAGGACTACCCAAACACAAGAAAGTTTGATAATATATCAGAAGTTGGAGACGACACGAGTGACAGAATTTATACCATTGACTCTGTTCACAACGGAGCACCTTGCAATGGTTATAGAGAAGTCAAATCGGGAGTAACTTCGAGGGGACTGATTGGGCCTGATTTTGAGGATCGATCATGA

Protein Analysis

388

Amino Acids

43.72

Weight (kDa)

4.92

Isoelectric Point (pI)

57.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zein-binding PF04576 71 - 161 2.9e-35 Zein-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 177
AccB7I CCANNNNNTGG 2 cut(s) 571, 763
AccI GTMKAC 2 cut(s) 260, 899
AccII CGCG 1 cut(s) 219
AccIII TCCGGA 1 cut(s) 869
AciI CCGC 3 cut(s) 297, 780, 856
AclWI GGATC 1 cut(s) 1161
AcoI YGGCCR 1 cut(s) 841
AcsI RAATTY 2 cut(s) 739, 1050
AcuI CTGAAG 2 cut(s) 69, 940
AcvI CACGTG 1 cut(s) 474
AfiI CCNNNNNNNGG 5 cut(s) 571, 720, 763, 794, 1091
AflIII ACRYGT 1 cut(s) 473
AgsI TTSAA 6 cut(s) 167, 376, 584, 634, 827, 882
AleI CACNNNNGTG 1 cut(s) 472
AluBI AGCT 3 cut(s) 268, 418, 954
AluI AGCT 3 cut(s) 268, 418, 954
Alw21I GWGCWC 1 cut(s) 1084
Alw26I GTCTC 5 cut(s) 215, 644, 704, 979, 1026
AlwI GGATC 1 cut(s) 1161
Ama87I CYCGRG 1 cut(s) 158
Aor13HI TCCGGA 1 cut(s) 869
AoxI GGCC 5 cut(s) 29, 363, 486, 841, 1139
ApeKI GCWGC 2 cut(s) 299, 951
ApoI RAATTY 2 cut(s) 739, 1050
Asp700I GAANNNNTTC 3 cut(s) 15, 286, 877
AspS9I GGNCC 3 cut(s) 364, 917, 1139
AsuC2I CCSGG 4 cut(s) 159, 160, 367, 777
AsuHPI GGTGA 5 cut(s) 49, 85, 551, 587, 882
AvaI CYCGRG 1 cut(s) 158
AvaII GGWCC 1 cut(s) 917
BalI TGGCCA 1 cut(s) 843
BanII GRGCYC 1 cut(s) 519
BauI CACGAG 3 cut(s) 183, 717, 1039
BbrPI CACGTG 1 cut(s) 474
BbsI GAAGAC 1 cut(s) 880
Bbv12I GWGCWC 1 cut(s) 1084
BbvI GCAGC 2 cut(s) 286, 963
BccI CCATC 7 cut(s) 28, 131, 352, 391, 757, 765, 784
BceAI ACGGC 1 cut(s) 543
BcgI CGANNNNNNTGC 4 cut(s) 191, 225, 662, 696
BciVI GTATCC 1 cut(s) 572
BcnI CCSGG 4 cut(s) 159, 160, 367, 777
BcoDI GTCTC 5 cut(s) 215, 644, 704, 979, 1026
BfaI CTAG 1 cut(s) 939
BfuI GTATCC 1 cut(s) 572
BglI GCCNNNNNGGC 1 cut(s) 308
BisI GCNGC 3 cut(s) 300, 781, 952
BlsI GCNGC 3 cut(s) 301, 782, 953
Bme1390I CCNGG 4 cut(s) 159, 160, 367, 777
Bme18I GGWCC 1 cut(s) 917
BmeT110I CYCGRG 1 cut(s) 158
BmgT120I GGNCC 3 cut(s) 364, 917, 1139
BmiI GGNNCC 3 cut(s) 550, 755, 811
BmrFI CCNGG 4 cut(s) 159, 160, 367, 777
BmrI ACTGGG 1 cut(s) 583
BmsI GCATC 1 cut(s) 670
BmuI ACTGGG 1 cut(s) 583
BpiI GAAGAC 1 cut(s) 880
BpuEI CTTGAG 1 cut(s) 10
BpuMI CCSGG 4 cut(s) 159, 160, 367, 777
Bsa29I ATCGAT 1 cut(s) 1156
BsaAI YACGTR 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 2 cut(s) 158, 851
BsaWI WCCGGW 1 cut(s) 869
Bsc4I CCNNNNNNNGG 5 cut(s) 571, 720, 763, 794, 1091
Bse1I ACTGG 3 cut(s) 432, 589, 919
Bse3DI GCAATG 1 cut(s) 1096
BseAI TCCGGA 1 cut(s) 869
BseCI ATCGAT 1 cut(s) 1156
BseDI CCNNGG 2 cut(s) 158, 851
BseGI GGATG 3 cut(s) 20, 142, 293
BseLI CCNNNNNNNGG 5 cut(s) 571, 720, 763, 794, 1091
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 3 cut(s) 255, 519, 975
BseNI ACTGG 3 cut(s) 432, 589, 919
BseRI GAGGAG 5 cut(s) 290, 404, 784, 810, 840
BseXI GCAGC 2 cut(s) 286, 963
BsgI GTGCAG 1 cut(s) 503
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 5 cut(s) 31, 365, 488, 843, 1141
BshVI ATCGAT 1 cut(s) 1156
BsiHKAI GWGCWC 1 cut(s) 1084
BsiHKCI CYCGRG 1 cut(s) 158
BsiSI CCGG 4 cut(s) 159, 367, 776, 870
BslFI GGGAC 2 cut(s) 106, 1143
BslI CCNNNNNNNGG 5 cut(s) 571, 720, 763, 794, 1091
BsmAI GTCTC 5 cut(s) 215, 644, 704, 979, 1026
BsmBI CGTCTC 3 cut(s) 215, 644, 1026
BsmFI GGGAC 2 cut(s) 106, 1143
BsnI GGCC 5 cut(s) 31, 365, 488, 843, 1141
Bso31I GGTCTC 1 cut(s) 704
BsoBI CYCGRG 1 cut(s) 158
Bsp1286I GDGCHC 2 cut(s) 519, 1084
Bsp13I TCCGGA 1 cut(s) 869
Bsp143I GATC 2 cut(s) 1153, 1157
BspACI CCGC 3 cut(s) 297, 780, 856
BspANI GGCC 5 cut(s) 31, 365, 488, 843, 1141
BspCNI CTCAG 3 cut(s) 256, 520, 976
BspDI ATCGAT 1 cut(s) 1156
BspEI TCCGGA 1 cut(s) 869
BspFNI CGCG 1 cut(s) 219
BspHI TCATGA 1 cut(s) 1160
BspLI GGNNCC 3 cut(s) 550, 755, 811
BspPI GGATC 1 cut(s) 1161
BspTNI GGTCTC 1 cut(s) 704
BsrDI GCAATG 1 cut(s) 1096
BsrI ACTGG 3 cut(s) 432, 589, 919
BssECI CCNNGG 2 cut(s) 158, 851
BssMI GATC 2 cut(s) 1153, 1157
BssNAI GTATAC 2 cut(s) 261, 900
BssSI CACGAG 3 cut(s) 183, 717, 1039
Bst1107I GTATAC 2 cut(s) 261, 900
Bst2BI CACGAG 3 cut(s) 183, 717, 1039
Bst4CI ACNGT 4 cut(s) 37, 146, 904, 981
BstBAI YACGTR 1 cut(s) 474
BstC8I GCNNGC 3 cut(s) 236, 486, 752
BstDEI CTNAG 4 cut(s) 227, 264, 528, 984
BstEII GGTNACC 1 cut(s) 140
BstF5I GGATG 3 cut(s) 20, 142, 293
BstFNI CGCG 1 cut(s) 219
BstKTI GATC 2 cut(s) 1156, 1160
BstMAI GTCTC 5 cut(s) 215, 644, 704, 979, 1026
BstMBI GATC 2 cut(s) 1153, 1157
BstMWI GCNNNNNNNGC 2 cut(s) 308, 464
BstPI GGTNACC 1 cut(s) 140
BstSCI CCNGG 4 cut(s) 157, 158, 365, 775
BstUI CGCG 1 cut(s) 219
BstV1I GCAGC 2 cut(s) 286, 963
BstV2I GAAGAC 1 cut(s) 880
BstZ17I GTATAC 2 cut(s) 261, 900
Bsu15I ATCGAT 1 cut(s) 1156
BsuI GTATCC 1 cut(s) 572
BsuRI GGCC 5 cut(s) 31, 365, 488, 843, 1141
BsuTUI ATCGAT 1 cut(s) 1156
BtgZI GCGATG 1 cut(s) 687
BtsCI GGATG 3 cut(s) 20, 142, 293
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 3 cut(s) 236, 486, 752
CciI TCATGA 1 cut(s) 1160
Cfr13I GGNCC 3 cut(s) 364, 917, 1139
Cfr9I CCCGGG 1 cut(s) 158
ClaI ATCGAT 1 cut(s) 1156
CseI GACGC 2 cut(s) 232, 661
CviAII CATG 2 cut(s) 404, 1161
DdeI CTNAG 4 cut(s) 227, 264, 528, 984
DpnI GATC 2 cut(s) 1155, 1159
DpnII GATC 2 cut(s) 1153, 1157
EaeI YGGCCR 1 cut(s) 841
Eco147I AGGCCT 1 cut(s) 488
Eco24I GRGCYC 1 cut(s) 519
Eco31I GGTCTC 1 cut(s) 704
Eco47I GGWCC 1 cut(s) 917
Eco57I CTGAAG 2 cut(s) 69, 940
Eco72I CACGTG 1 cut(s) 474
Eco88I CYCGRG 1 cut(s) 158
Eco91I GGTNACC 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 140
EcoT22I ATGCAT 1 cut(s) 703
EcoT38I GRGCYC 1 cut(s) 519
Esp3I CGTCTC 3 cut(s) 215, 644, 1026
FaeI CATG 2 cut(s) 407, 1164
FaqI GGGAC 2 cut(s) 106, 1143
FatI CATG 2 cut(s) 403, 1160
FauNDI CATATG 1 cut(s) 616
FblI GTMKAC 2 cut(s) 260, 899
Fnu4HI GCNGC 3 cut(s) 300, 781, 952
FokI GGATG 3 cut(s) 7, 149, 280
FriOI GRGCYC 1 cut(s) 519
Fsp4HI GCNGC 3 cut(s) 300, 781, 952
FspBI CTAG 1 cut(s) 939
GluI GCNGC 3 cut(s) 300, 781, 952
HaeIII GGCC 5 cut(s) 31, 365, 488, 843, 1141
HapII CCGG 4 cut(s) 159, 367, 776, 870
HgaI GACGC 2 cut(s) 232, 661
Hin1II CATG 2 cut(s) 407, 1164
HinfI GANTC 8 cut(s) 5, 186, 460, 524, 772, 887, 932, 1064
HpaII CCGG 4 cut(s) 159, 367, 776, 870
HphI GGTGA 5 cut(s) 49, 85, 551, 587, 882
Hpy166II GTNNAC 3 cut(s) 261, 900, 1072
Hpy188I TCNGA 7 cut(s) 10, 529, 603, 762, 789, 985, 1024
Hpy188III TCNNGA 7 cut(s) 565, 861, 870, 920, 958, 1113, 1161
Hpy8I GTNNAC 3 cut(s) 261, 900, 1072
Hpy99I CGWCG 1 cut(s) 37
HpyAV CCTTC 2 cut(s) 337, 955
HpyCH4III ACNGT 4 cut(s) 37, 146, 904, 981
HpyCH4IV ACGT 1 cut(s) 473
HpyCH4V TGCA 8 cut(s) 182, 212, 334, 484, 662, 683, 701, 1089
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 464
HpyF3I CTNAG 4 cut(s) 227, 264, 528, 984
HpySE526I ACGT 1 cut(s) 473
Hsp92II CATG 2 cut(s) 407, 1164
Kpn2I TCCGGA 1 cut(s) 869
Kzo9I GATC 2 cut(s) 1153, 1157
LmnI GCTCC 3 cut(s) 415, 759, 1079
Lsp1109I GCAGC 2 cut(s) 286, 963
LweI GCATC 1 cut(s) 670
MaeI CTAG 1 cut(s) 939
MaeII ACGT 1 cut(s) 473
MaeIII GTNAC 5 cut(s) 140, 575, 884, 1043, 1117
MalI GATC 2 cut(s) 1155, 1159
MboI GATC 2 cut(s) 1153, 1157
MboII GAAGA 4 cut(s) 75, 406, 443, 885
MhlI GDGCHC 2 cut(s) 519, 1084
MlsI TGGCCA 1 cut(s) 843
MluCI AATT 4 cut(s) 49, 371, 739, 1050
MluNI TGGCCA 1 cut(s) 843
MlyI GAGTC 5 cut(s) 195, 533, 781, 881, 1058
MmeI TCCRAC 2 cut(s) 626, 1009
Mox20I TGGCCA 1 cut(s) 843
Mph1103I ATGCAT 1 cut(s) 703
MroI TCCGGA 1 cut(s) 869
MroXI GAANNNNTTC 3 cut(s) 15, 286, 877
MscI TGGCCA 1 cut(s) 843
MseI TTAA 1 cut(s) 906
MslI CAYNNNNRTG 3 cut(s) 472, 667, 882
Msp20I TGGCCA 1 cut(s) 843
MspA1I CMGCKG 1 cut(s) 299
MspI CCGG 4 cut(s) 159, 367, 776, 870
MspR9I CCNGG 4 cut(s) 159, 160, 367, 777
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 2 cut(s) 308, 464
NciI CCSGG 4 cut(s) 159, 160, 367, 777
NdeI CATATG 1 cut(s) 616
NdeII GATC 2 cut(s) 1153, 1157
NlaIII CATG 2 cut(s) 407, 1164
NlaIV GGNNCC 3 cut(s) 550, 755, 811
NmeAIII GCCGAG 1 cut(s) 832
NmuCI GTSAC 3 cut(s) 575, 884, 1043
NsiI ATGCAT 1 cut(s) 703
OliI CACNNNNGTG 1 cut(s) 472
PagI TCATGA 1 cut(s) 1160
PceI AGGCCT 1 cut(s) 488
PdmI GAANNNNTTC 3 cut(s) 15, 286, 877
PfeI GAWTC 3 cut(s) 5, 460, 932
PflFI GACNNNGTC 1 cut(s) 971
PflMI CCANNNNNTGG 2 cut(s) 571, 763
PkrI GCNGC 3 cut(s) 301, 782, 953
PleI GAGTC 5 cut(s) 194, 532, 780, 881, 1058
PmaCI CACGTG 1 cut(s) 474
PmlI CACGTG 1 cut(s) 474
PpsI GAGTC 5 cut(s) 194, 532, 780, 881, 1058
Ppu21I YACGTR 1 cut(s) 474
PsiI TTATAA 1 cut(s) 177
PspCI CACGTG 1 cut(s) 474
PspEI GGTNACC 1 cut(s) 140
PspN4I GGNNCC 3 cut(s) 550, 755, 811
PspPI GGNCC 3 cut(s) 364, 917, 1139
PsyI GACNNNGTC 1 cut(s) 971
RseI CAYNNNNRTG 3 cut(s) 472, 667, 882
SaqAI TTAA 1 cut(s) 906
SatI GCNGC 3 cut(s) 300, 781, 952
Sau3AI GATC 2 cut(s) 1153, 1157
Sau96I GGNCC 3 cut(s) 364, 917, 1139
SchI GAGTC 5 cut(s) 195, 533, 781, 881, 1058
ScrFI CCNGG 4 cut(s) 159, 160, 367, 777
SduI GDGCHC 2 cut(s) 519, 1084
SfaNI GCATC 1 cut(s) 670
SinI GGWCC 1 cut(s) 917
SmaI CCCGGG 1 cut(s) 160
SmiMI CAYNNNNRTG 3 cut(s) 472, 667, 882
SmlI CTYRAG 1 cut(s) 25
SmoI CTYRAG 1 cut(s) 25
Sse9I AATT 4 cut(s) 49, 371, 739, 1050
SseBI AGGCCT 1 cut(s) 488
SsiI CCGC 3 cut(s) 297, 780, 856
SspMI CTAG 1 cut(s) 939
StuI AGGCCT 1 cut(s) 488
StyD4I CCNGG 4 cut(s) 157, 158, 365, 775
TaaI ACNGT 4 cut(s) 37, 146, 904, 981
TaiI ACGT 1 cut(s) 476
TaqI TCGA 2 cut(s) 1124, 1156
TaqII GACCGA 1 cut(s) 93
TasI AATT 4 cut(s) 49, 371, 739, 1050
TauI GCSGC 1 cut(s) 783
TfiI GAWTC 3 cut(s) 5, 460, 932
Tru1I TTAA 1 cut(s) 906
Tru9I TTAA 1 cut(s) 906
TscAI CASTG 1 cut(s) 432
TseFI GTSAC 3 cut(s) 575, 884, 1043
TseI GCWGC 2 cut(s) 299, 951
Tsp45I GTSAC 3 cut(s) 575, 884, 1043
TspDTI ATGAA 3 cut(s) 279, 609, 633
TspGWI ACGGA 2 cut(s) 536, 1092
TspMI CCCGGG 1 cut(s) 158
TspRI CASTG 1 cut(s) 432
Tth111I GACNNNGTC 1 cut(s) 971
Van91I CCANNNNNTGG 2 cut(s) 571, 763
VpaK11BI GGWCC 1 cut(s) 917
XapI RAATTY 2 cut(s) 739, 1050
XmaI CCCGGG 1 cut(s) 158
XmiI GTMKAC 2 cut(s) 260, 899
XmnI GAANNNNTTC 3 cut(s) 15, 286, 877
XspI CTAG 1 cut(s) 939
Zsp2I ATGCAT 1 cut(s) 703
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.