AT5G10020
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016140
Physical Location & Seq
Forward (+)
3133244 .. 3137242
3999 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10020.1

Sequence Viewer

Length: 3147 bp
ATGAGTCATTTTCTCACTTTCTGCTTCCTCTCACTCCTTCTCCTCCTCCACGGTGCCAACGCCGTCACCGAGACGGAGCTCCGATCTCTACTCGAGTTCCGTAAAGGGATCCGTGACGAGACCTCACACCAACGAATCTCATGGTCAGATACAAGCAGCCTCACTGATCCATCTACTTGCCCTAATGACTGGCCTGGTATCTCTTGCGACCCTGAAACCGGTTCAATCATCGCCATTAATCTCGACCGTCGTGGTCTCTCCGGCGAACTCAAATTCAGCACTCTCTCTGGTCTTACTCGTCTTCGTAACCTCTCTTTATCCGGAAACAGCTTCTCGGGTCGGGTCGTTCCTTCATTGGGTGGAATCTCGTCGCTTCAACACTTAGATCTGTCGGATAACGGTTTCTACGGACCTATACCGGGTCGGATCTCGGAGCTTTGGAGTTTGAATCATCTCAATTTGTCTTCCAACAAGTTCGAAGGTGGGTTTCCTAGTGGCTTTCGGAATCTTCAGCAGCTAAGATCTTTGGATTTGCATAAGAACGAGATTTGGGGAGATGTTGGTGAGATTTTCACTGAGCTTAAGAATGTTGAGTTCGTTGATTTGAGCTGTAACCGTTTCAACGGTGGCTTATCTTTACCAATGGAGAACATTTCCAGCATTTCCAACACTCTCCGTCACTTGAATTTGAGCCACAATGCGTTAAATGGCAAGTTTTTCAGTGAGGAGTCCATTGGTTCGTTCAAGAATCTCGAGATTGTTGACTTAGAGAATAACCAGATCAATGGTGAGTTACCGCATTTTGGATCACAGCCGAGTTTGAGAATATTGAAGCTTGCTCGTAACGAGTTGTTCGGTTTAGTACCTCAAGAGTTGTTGCAGAGTTCGATTCCTTTGCTAGAGCTAGATCTAAGTCGAAACGGTTTTACAGGATCAATTAGTGAAATCAATTCATCAACGTTAACTATGTTGAATCTTTCTTCTAACGGCCTGTCTGGGGATCTTCCGTCGAGTTTTAAAAGTTGCTCAGTGATAGATTTGAGTGGGAATACTTTCTCTGGCGACGTGTCTGTTGTTCAAAAGTGGGAAGCTACTCCAGATGTTCTTGATCTTAGCTCAAACAACTTGTCGGGAAGCTTGCCGAACTTTACTTCTGCATTTTCAAGATTAAGTGTGTTGAGCATTAGGAATAATTCTGTCTCTGGTAGCTTGCCTTCTCTGTGGGGTGATTCTCAATTCTCTGTGATTGATCTCAGTTCAAATAAGTTCAGTGGCTTCATTCCAGTGAGTTTCTTCACTTTTGCAAGCTTGAGAAGTTTGAATCTTTCTAGGAATAACTTGGAGGGGCCAATTCCTTTTCGAGGTTCACGTGCAAGCGAACTTTTGGTTCTAAATTCTTATCCGCAGATGGAGTTACTTGACCTTTCAACCAATTCTTTGACGGGTATGCTGCCTGGGGATATAGGTACAATGGAAAAAATTAAAGTGCTGAATCTTGCCAATAATAAGCTGTCCGGGGAACTTCCAAGCGACCTCAACAAGCTTAGTGGCCTTTTGTTCCTTGACTTGTCAAACAATACTTTCAAGGGTCAAATTCCCAATAAGCTTCCTTCCCAAATGGTGGGATTCAATGTGTCGTATAACGACCTATCAGGCATAATTCCGGAAGACCTTAGAAGCTACCCGCCTTCTTCATTTTATCCTGGTAACTCCAAGCTAAGTCTCCCAGGACGGATTCCAGCGGATTCTTCTGGTGATCTGTCTTTACCTGGGAAAAAACATCATTCGAAGCTTAGCATAAGAATAGCGATTATTGTTGCTTCAGTTGGAGCTGCTATTATGATTCTTTTTGTCTTGTTTGCCTATCATCGAACACAGCTCAAGGACTTTCACGGGAGGAATAGATTTACAGACCAAGCAACGACTAGAGACACCAAGTTTGGACGCTCTTCTCGTCCCTCCTTATTCAACTTCAGCTCAAATGTTGAGCAGCAGTCATCATCTTTGAGCTTCTCAAACGATCACTTGCTCACCGCAAATTCAAGGTCGTTATCTGGGATACCCGGATGTGAAGCTGAAATATCCGAGCAGGGTGCGCCTGCTACTTCTGCTCCTACAAACCTCCTTGATGATTATCCTGCAGCATCTGGGCGCAAGTCTTCTTCAGGAGGCTCCCCACTATCCTCCTCACCCCGTTTTAGCGACCAGCCTGTGATGTTAGATGTTTACTCACCTGATAGATTGGCCGGAGAACTCTTCTTCTTAGACGTTTCACTAAAACTCACAGCAGAAGAGTTATCCCGAGCTCCTGCAGAAGTTCTAGGTAGAAGCAGCCATGGAACACTGTACAAAGCAACACTGGATAATGGACACATGTTGACGGTGAAATGGTTGAGAGTTGGCCTCGTGAGGCATAAGAAAGATTTCGCACGGGAAGCCAAAAAAATCGGCTCTTTAAAACATCCTAACATTGTTCCGTTGCGAGCATACTATTGGGGTCCCAGAGAACAAGAAAGACTTCTCCTGTCTGATTACCTGAGAGGAGAGAGCTTAGCCATGCATCTTTACGAGACTACTCCTCGGAGGTATTCTCCAATGTCCTTCAGCCAAAGACTAAAAGTTGCAGTTGAAGTGGCTCAGTGTCTTCTCTACCTTCATGATAGAGCAATGCCACACGGGAACTTGAAGCCAACTAACATAATCCTCTCAAGTCCTGACAATACCGTGCGAATAACTGATTACTGTGTTCACCGTTTGATGACTCCATCTGGTGTAGCGGAACAGATACTAAACATGAGTGCACTCGGTTACTCTGCTCCTGAACTTTCCTCGGCTTCCAAACCGATACCAACACTCAAATCCGATGTCTATGCGTTTGGTGTGATTCTCATGGAGCTTTTGACTAGACGGAGCGCAGGTGACATAATCTCAGGTCAAACAGGAGCCGTTGATCTGACCGATTGGGTGAGGTTGTGTGATCAAGAAGGAAGGAGAATGGATTGCATAGACAGAGACATAGCAGGTGGGGAAGAGTTCTCAAAGGGAATGGAGGATGCACTTGCTGTTGCAATCAGGTGCATTCTCTCTGTCAATGAAAGGCCTAACATCAGACAAGTTCTTGATCATCTTACTTCAATTTCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1048

Amino Acids

114.71

Weight (kDa)

6.32

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 85 - 182 8.2e-06 Leucine-rich repeat region
LRR_8 PF13855 148 - 207 2.3e-06 Leucine rich repeat
Pkinase PF00069 772 - 1042 4.7e-25 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 772 - 969 9.5e-25 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10020 AT5G10020
fragaria_vesca FvH4_1g22060
malus_domestica MD01G1018200.v1.1
pyrus_communis pycom01g04790
rosa_chinensis RchiOBHm_Chr2g0116131
rosa_laevigata RLG00000018242
rosa_multiflora Rmu_sc0002288.1_g000016
rosa_roxburghii Rroxscaffold_2G00127470
rosa_rugosa Rorug02G0203700
rosa_samantha Rh2AG260100 Rh2BG271100 Rh2CG266400 Rh2DG268300
rosa_wichuraiana Rw2G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 2906, 3011
Acc36I ACCTGC 2 cut(s) 2906, 3011
AccB1I GGYRCC 1 cut(s) 53
AccB7I CCANNNNNTGG 1 cut(s) 1621
AccIII TCCGGA 2 cut(s) 320, 1663
AciI CCGC 6 cut(s) 797, 1403, 1685, 1742, 2034, 2777
AclI AACGTT 1 cut(s) 959
AclWI GGATC 7 cut(s) 103, 116, 161, 434, 814, 940, 1008
AcoI YGGCCR 1 cut(s) 2244
AcsI RAATTY 5 cut(s) 272, 685, 1393, 1593, 2038
AcuI CTGAAG 5 cut(s) 494, 1806, 1957, 2148, 2587
AcvI CACGTG 1 cut(s) 1370
AfaI GTAC 3 cut(s) 864, 1468, 2348
AfiI CCNNNNNNNGG 7 cut(s) 218, 356, 419, 803, 997, 1361, 1621
AflII CTTAAG 1 cut(s) 581
AflIII ACRYGT 2 cut(s) 1065, 2373
AgeI ACCGGT 1 cut(s) 218
AjiI CACGTC 1 cut(s) 1066
AjnI CCWGG 5 cut(s) 193, 1453, 1702, 1726, 1768
Alw21I GWGCWC 3 cut(s) 81, 2308, 2803
Alw26I GTCTC 8 cut(s) 65, 113, 260, 1204, 1727, 1923, 2564, 3006
Alw44I GTGCAC 1 cut(s) 2799
AlwI GGATC 7 cut(s) 103, 116, 161, 434, 814, 940, 1008
AlwNI CAGNNNCTG 1 cut(s) 2147
Ama87I CYCGRG 4 cut(s) 92, 334, 752, 2301
Aor13HI TCCGGA 2 cut(s) 320, 1663
AoxI GGCC 7 cut(s) 191, 988, 1346, 1549, 2244, 2401, 3098
ApaLI GTGCAC 1 cut(s) 2799
ApeKI GCWGC 7 cut(s) 156, 514, 1450, 1832, 1990, 2141, 2331
ApoI RAATTY 5 cut(s) 272, 685, 1393, 1593, 2038
ArsI GACNNNNNNTTYG 8 cut(s) 1112, 1144, 1922, 1954, 2030, 2062, 2850, 2882
AseI ATTAAT 1 cut(s) 237
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 3 cut(s) 1052, 2423, 2517
AspLEI GCGC 3 cut(s) 2098, 2154, 2915
AspS9I GGNCC 3 cut(s) 410, 1346, 2498
AsuC2I CCSGG 3 cut(s) 420, 1516, 2064
AsuII TTCGAA 2 cut(s) 477, 1787
AvaI CYCGRG 4 cut(s) 92, 334, 752, 2301
AvaII GGWCC 2 cut(s) 410, 2498
BaeGI GKGCMC 1 cut(s) 2803
BamHI GGATCC 1 cut(s) 108
BanI GGYRCC 1 cut(s) 53
BanII GRGCYC 2 cut(s) 81, 2308
BarI GAAGNNNNNNTAC 2 cut(s) 1198, 1230
BauI CACGAG 1 cut(s) 2405
BbrPI CACGTG 1 cut(s) 1370
BbsI GAAGAC 5 cut(s) 293, 456, 1674, 2151, 2636
Bbv12I GWGCWC 3 cut(s) 81, 2308, 2803
BbvI GCAGC 7 cut(s) 168, 526, 1437, 1819, 2002, 2153, 2343
BccI CCATC 3 cut(s) 178, 1402, 2773
BceAI ACGGC 3 cut(s) 47, 1003, 2930
BcgI CGANNNNNNTGC 6 cut(s) 877, 911, 2075, 2109, 2852, 2886
BciT130I CCWGG 5 cut(s) 195, 1455, 1704, 1728, 1770
BciVI GTATCC 1 cut(s) 2052
BclI TGATCA 2 cut(s) 2977, 3121
BcnI CCSGG 3 cut(s) 420, 1516, 2064
BcoDI GTCTC 8 cut(s) 65, 113, 260, 1204, 1727, 1923, 2564, 3006
BfaI CTAG 7 cut(s) 492, 899, 905, 1329, 1926, 2321, 2904
BfmI CTRYAG 2 cut(s) 2139, 2310
BfrI CTTAAG 1 cut(s) 581
BfuAI ACCTGC 2 cut(s) 2906, 3011
BfuI GTATCC 1 cut(s) 2052
BglII AGATCT 3 cut(s) 385, 521, 907
BisI GCNGC 7 cut(s) 157, 515, 1451, 1833, 1991, 2142, 2332
BlpI GCTNAGC 2 cut(s) 1793, 2551
BlsI GCNGC 7 cut(s) 158, 516, 1452, 1834, 1992, 2143, 2333
Bme1390I CCNGG 8 cut(s) 195, 420, 1455, 1516, 1704, 1728, 1770, 2064
Bme18I GGWCC 2 cut(s) 410, 2498
BmeT110I CYCGRG 4 cut(s) 92, 334, 752, 2301
BmgBI CACGTC 1 cut(s) 1066
BmgT120I GGNCC 3 cut(s) 410, 1346, 2498
BmiI GGNNCC 7 cut(s) 55, 110, 1347, 2173, 2499, 2500, 2944
BmrFI CCNGG 8 cut(s) 195, 420, 1455, 1516, 1704, 1728, 1770, 2064
BmsI GCATC 3 cut(s) 2153, 2569, 3043
BpiI GAAGAC 5 cut(s) 293, 456, 1674, 2151, 2636
BplI GAGNNNNNCTC 4 cut(s) 2388, 2420, 2575, 2607
BpmI CTGGAG 1 cut(s) 1080
Bpu1102I GCTNAGC 2 cut(s) 1793, 2551
Bpu14I TTCGAA 2 cut(s) 477, 1787
BpuEI CTTGAG 4 cut(s) 852, 1330, 1865, 2692
BpuMI CCSGG 3 cut(s) 420, 1516, 2064
BsaAI YACGTR 1 cut(s) 1370
BsaBI GATNNNNATC 1 cut(s) 2133
BsaI GGTCTC 2 cut(s) 113, 260
BsaJI CCNNGG 8 cut(s) 49, 1454, 1515, 1726, 1769, 2335, 2579, 2829
BsaWI WCCGGW 3 cut(s) 218, 320, 1663
BsaXI ACNNNNNCTCC 6 cut(s) 24, 54, 2095, 2125, 2902, 2932
Bsc4I CCNNNNNNNGG 7 cut(s) 218, 356, 419, 803, 997, 1361, 1621
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 3 cut(s) 194, 1283, 2364
Bse3DI GCAATG 1 cut(s) 2673
Bse8I GATNNNNATC 1 cut(s) 2133
BseAI TCCGGA 2 cut(s) 320, 1663
BseBI CCWGG 5 cut(s) 195, 1455, 1704, 1728, 1770
BseDI CCNNGG 8 cut(s) 49, 1454, 1515, 1726, 1769, 2335, 2579, 2829
BseGI GGATG 3 cut(s) 2072, 2461, 3058
BseJI GATNNNNATC 1 cut(s) 2133
BseLI CCNNNNNNNGG 7 cut(s) 218, 356, 419, 803, 997, 1361, 1621
BseMI GCAATG 1 cut(s) 2673
BseMII CTCAG 6 cut(s) 567, 1041, 1267, 2528, 2651, 2943
BseNI ACTGG 3 cut(s) 194, 1283, 2364
BseRI GAGGAG 6 cut(s) 32, 35, 740, 2176, 2556, 2568
BseSI GKGCMC 1 cut(s) 2803
BseXI GCAGC 7 cut(s) 168, 526, 1437, 1819, 2002, 2153, 2343
Bsh1285I CGRYCG 1 cut(s) 247
BshFI GGCC 7 cut(s) 193, 990, 1348, 1551, 2246, 2403, 3100
BshNI GGYRCC 1 cut(s) 53
BshTI ACCGGT 1 cut(s) 218
BsiEI CGRYCG 1 cut(s) 247
BsiHKAI GWGCWC 3 cut(s) 81, 2308, 2803
BsiHKCI CYCGRG 4 cut(s) 92, 334, 752, 2301
BsiSI CCGG 8 cut(s) 219, 261, 321, 419, 1515, 1664, 2064, 2247
BslFI GGGAC 2 cut(s) 1941, 2484
BslI CCNNNNNNNGG 7 cut(s) 218, 356, 419, 803, 997, 1361, 1621
BsmAI GTCTC 8 cut(s) 65, 113, 260, 1204, 1727, 1923, 2564, 3006
BsmBI CGTCTC 1 cut(s) 65
BsmFI GGGAC 2 cut(s) 1941, 2484
BsmI GAATGC 1 cut(s) 3078
BsnI GGCC 7 cut(s) 193, 990, 1348, 1551, 2246, 2403, 3100
Bso31I GGTCTC 2 cut(s) 113, 260
BsoBI CYCGRG 4 cut(s) 92, 334, 752, 2301
Bsp119I TTCGAA 2 cut(s) 477, 1787
Bsp1286I GDGCHC 3 cut(s) 81, 2308, 2803
Bsp13I TCCGGA 2 cut(s) 320, 1663
Bsp1407I TGTACA 1 cut(s) 2346
Bsp1720I GCTNAGC 2 cut(s) 1793, 2551
Bsp19I CCATGG 1 cut(s) 2335
BspACI CCGC 6 cut(s) 797, 1403, 1685, 1742, 2034, 2777
BspANI GGCC 7 cut(s) 193, 990, 1348, 1551, 2246, 2403, 3100
BspCNI CTCAG 6 cut(s) 568, 1040, 1266, 2529, 2650, 2942
BspEI TCCGGA 2 cut(s) 320, 1663
BspHI TCATGA 1 cut(s) 2656
BspLI GGNNCC 7 cut(s) 55, 110, 1347, 2173, 2499, 2500, 2944
BspMAI CTGCAG 2 cut(s) 2143, 2314
BspMI ACCTGC 2 cut(s) 2906, 3011
BspPI GGATC 7 cut(s) 103, 116, 161, 434, 814, 940, 1008
BspQI GCTCTTC 1 cut(s) 1954
BspT104I TTCGAA 2 cut(s) 477, 1787
BspT107I GGYRCC 1 cut(s) 53
BspTI CTTAAG 1 cut(s) 581
BspTNI GGTCTC 2 cut(s) 113, 260
BsrDI GCAATG 1 cut(s) 2673
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 2346
BsrI ACTGG 3 cut(s) 194, 1283, 2364
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 8 cut(s) 49, 1454, 1515, 1726, 1769, 2335, 2579, 2829
BssSI CACGAG 1 cut(s) 2405
BssT1I CCWWGG 1 cut(s) 2335
Bst2BI CACGAG 1 cut(s) 2405
Bst2UI CCWGG 5 cut(s) 195, 1455, 1704, 1728, 1770
Bst6I CTCTTC 4 cut(s) 1954, 2261, 2286, 3024
BstAFI CTTAAG 1 cut(s) 581
BstAUI TGTACA 1 cut(s) 2346
BstBAI YACGTR 1 cut(s) 1370
BstBI TTCGAA 2 cut(s) 477, 1787
BstC8I GCNNGC 7 cut(s) 837, 1139, 1211, 1306, 1375, 2100, 2484
BstDSI CCRYGG 2 cut(s) 49, 2335
BstENI CCTNNNNNAGG 1 cut(s) 1359
BstF5I GGATG 3 cut(s) 2072, 2461, 3058
BstHHI GCGC 3 cut(s) 2098, 2154, 2915
BstMAI GTCTC 8 cut(s) 65, 113, 260, 1204, 1727, 1923, 2564, 3006
BstMCI CGRYCG 1 cut(s) 247
BstMWI GCNNNNNNNGC 3 cut(s) 2095, 2108, 2435
BstNI CCWGG 5 cut(s) 195, 1455, 1704, 1728, 1770
BstNSI RCATGY 1 cut(s) 2377
BstSCI CCNGG 8 cut(s) 193, 418, 1453, 1514, 1702, 1726, 1768, 2062
BstSFI CTRYAG 2 cut(s) 2139, 2310
BstSLI GKGCMC 1 cut(s) 2803
BstV1I GCAGC 7 cut(s) 168, 526, 1437, 1819, 2002, 2153, 2343
BstV2I GAAGAC 5 cut(s) 293, 456, 1674, 2151, 2636
BstX2I RGATCY 6 cut(s) 108, 385, 426, 521, 907, 1000
BstXI CCANNNNNNTGG 1 cut(s) 785
BstYI RGATCY 6 cut(s) 108, 385, 426, 521, 907, 1000
BsuI GTATCC 1 cut(s) 2052
BsuRI GGCC 7 cut(s) 193, 990, 1348, 1551, 2246, 2403, 3100
BtgI CCRYGG 2 cut(s) 49, 2335
BtgZI GCGATG 1 cut(s) 214
BtrI CACGTC 1 cut(s) 1066
BtsCI GGATG 3 cut(s) 2072, 2461, 3058
BtsIMutI CAGTG 9 cut(s) 162, 573, 727, 1035, 1276, 1290, 2342, 2357, 2645
BveI ACCTGC 2 cut(s) 2906, 3011
Cac8I GCNNGC 7 cut(s) 837, 1139, 1211, 1306, 1375, 2100, 2484
CaiI CAGNNNCTG 1 cut(s) 2147
CciI TCATGA 1 cut(s) 2656
CfoI GCGC 3 cut(s) 2098, 2154, 2915
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 3 cut(s) 410, 1346, 2498
CseI GACGC 1 cut(s) 1953
Csp6I GTAC 3 cut(s) 863, 1467, 2347
CspAI ACCGGT 1 cut(s) 218
CspCI CAANNNNNGTGG 2 cut(s) 1528, 1563
CviAII CATG 7 cut(s) 141, 2336, 2374, 2557, 2657, 2794, 2890
CviQI GTAC 3 cut(s) 863, 1467, 2347
DraI TTTAAA 2 cut(s) 1018, 2457
EaeI YGGCCR 1 cut(s) 2244
Eam1104I CTCTTC 4 cut(s) 1954, 2261, 2286, 3024
EarI CTCTTC 4 cut(s) 1954, 2261, 2286, 3024
Ecl136II GAGCTC 2 cut(s) 79, 2306
Eco130I CCWWGG 1 cut(s) 2335
Eco147I AGGCCT 1 cut(s) 3100
Eco24I GRGCYC 2 cut(s) 81, 2308
Eco31I GGTCTC 2 cut(s) 113, 260
Eco47I GGWCC 2 cut(s) 410, 2498
Eco53kI GAGCTC 2 cut(s) 79, 2306
Eco57I CTGAAG 5 cut(s) 494, 1806, 1957, 2148, 2587
Eco72I CACGTG 1 cut(s) 1370
Eco88I CYCGRG 4 cut(s) 92, 334, 752, 2301
EcoICRI GAGCTC 2 cut(s) 79, 2306
EcoNI CCTNNNNNAGG 1 cut(s) 1359
EcoO109I RGGNCCY 1 cut(s) 2498
EcoRII CCWGG 5 cut(s) 193, 1453, 1702, 1726, 1768
EcoT14I CCWWGG 1 cut(s) 2335
EcoT22I ATGCAT 1 cut(s) 2562
EcoT38I GRGCYC 2 cut(s) 81, 2308
ErhI CCWWGG 1 cut(s) 2335
Esp3I CGTCTC 1 cut(s) 65
FaeI CATG 7 cut(s) 144, 2339, 2377, 2560, 2660, 2797, 2893
FalI AAGNNNNNCTT 2 cut(s) 2502, 2534
FaqI GGGAC 2 cut(s) 1941, 2484
FatI CATG 7 cut(s) 140, 2335, 2373, 2556, 2656, 2793, 2889
FauI CCCGC 1 cut(s) 1692
FbaI TGATCA 2 cut(s) 2977, 3121
Fnu4HI GCNGC 7 cut(s) 157, 515, 1451, 1833, 1991, 2142, 2332
FokI GGATG 3 cut(s) 2079, 2448, 3065
FriOI GRGCYC 2 cut(s) 81, 2308
Fsp4HI GCNGC 7 cut(s) 157, 515, 1451, 1833, 1991, 2142, 2332
FspBI CTAG 7 cut(s) 492, 899, 905, 1329, 1926, 2321, 2904
GlaI GCGC 3 cut(s) 2097, 2153, 2914
GluI GCNGC 7 cut(s) 157, 515, 1451, 1833, 1991, 2142, 2332
GsuI CTGGAG 1 cut(s) 1080
HaeIII GGCC 7 cut(s) 193, 990, 1348, 1551, 2246, 2403, 3100
HapII CCGG 8 cut(s) 219, 261, 321, 419, 1515, 1664, 2064, 2247
HgaI GACGC 1 cut(s) 1953
HhaI GCGC 3 cut(s) 2098, 2154, 2915
Hin1II CATG 7 cut(s) 144, 2339, 2377, 2560, 2660, 2797, 2893
Hin6I GCGC 3 cut(s) 2096, 2152, 2913
HinP1I GCGC 3 cut(s) 2096, 2152, 2913
HincII GTYRAC 3 cut(s) 763, 963, 2379
HindII GTYRAC 3 cut(s) 763, 963, 2379
HindIII AAGCTT 6 cut(s) 833, 1135, 1306, 1541, 1604, 1790
HpaI GTTAAC 1 cut(s) 963
HpaII CCGG 8 cut(s) 219, 261, 321, 419, 1515, 1664, 2064, 2247
Hpy166II GTNNAC 7 cut(s) 763, 963, 1367, 2227, 2379, 2749, 2801
Hpy8I GTNNAC 7 cut(s) 763, 963, 1367, 2227, 2379, 2749, 2801
Hpy99I CGWCG 4 cut(s) 252, 373, 1012, 1067
HpyCH4IV ACGT 4 cut(s) 959, 1065, 1369, 2268
HpyF10VI GCNNNNNNNGC 3 cut(s) 2095, 2108, 2435
HpySE526I ACGT 4 cut(s) 959, 1065, 1369, 2268
Hsp92II CATG 7 cut(s) 144, 2339, 2377, 2560, 2660, 2797, 2893
HspAI GCGC 3 cut(s) 2096, 2152, 2913
KflI GGGWCCC 1 cut(s) 2498
Kpn2I TCCGGA 2 cut(s) 320, 1663
Ksp22I TGATCA 2 cut(s) 2977, 3121
KspAI GTTAAC 1 cut(s) 963
LguI GCTCTTC 1 cut(s) 1954
Lsp1109I GCAGC 7 cut(s) 168, 526, 1437, 1819, 2002, 2153, 2343
LweI GCATC 3 cut(s) 2153, 2569, 3043
MaeI CTAG 7 cut(s) 492, 899, 905, 1329, 1926, 2321, 2904
MaeII ACGT 4 cut(s) 959, 1065, 1369, 2268
MflI RGATCY 6 cut(s) 108, 385, 426, 521, 907, 1000
MhlI GDGCHC 3 cut(s) 81, 2308, 2803
MlyI GAGTC 3 cut(s) 13, 737, 2755
MmeI TCCRAC 5 cut(s) 372, 404, 492, 690, 1807
Mph1103I ATGCAT 1 cut(s) 2562
MroI TCCGGA 2 cut(s) 320, 1663
MroXI GAANNNNTTC 3 cut(s) 1052, 2423, 2517
MseI TTAA 8 cut(s) 237, 582, 704, 962, 1017, 1169, 1482, 2456
MslI CAYNNNNRTG 1 cut(s) 1283
MspA1I CMGCKG 1 cut(s) 1742
MspCI CTTAAG 1 cut(s) 581
MspI CCGG 8 cut(s) 219, 261, 321, 419, 1515, 1664, 2064, 2247
MspR9I CCNGG 8 cut(s) 195, 420, 1455, 1516, 1704, 1728, 1770, 2064
Mva1269I GAATGC 1 cut(s) 3078
MvaI CCWGG 5 cut(s) 195, 1455, 1704, 1728, 1770
MwoI GCNNNNNNNGC 3 cut(s) 2095, 2108, 2435
NciI CCSGG 3 cut(s) 420, 1516, 2064
NcoI CCATGG 1 cut(s) 2335
NlaIII CATG 7 cut(s) 144, 2339, 2377, 2560, 2660, 2797, 2893
NlaIV GGNNCC 7 cut(s) 55, 110, 1347, 2173, 2499, 2500, 2944
NmeAIII GCCGAG 2 cut(s) 840, 2810
NmuCI GTSAC 4 cut(s) 64, 113, 677, 2918
NsiI ATGCAT 1 cut(s) 2562
NspI RCATGY 1 cut(s) 2377
NspV TTCGAA 2 cut(s) 477, 1787
PaeR7I CTCGAG 2 cut(s) 92, 752
PagI TCATGA 1 cut(s) 2656
PaqCI CACCTGC 2 cut(s) 2906, 3011
PceI AGGCCT 1 cut(s) 3100
PciI ACATGT 1 cut(s) 2373
PciSI GCTCTTC 1 cut(s) 1954
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 1366, 1951
PctI GAATGC 1 cut(s) 3078
PdmI GAANNNNTTC 3 cut(s) 1052, 2423, 2517
PflMI CCANNNNNTGG 1 cut(s) 1621
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 7 cut(s) 158, 516, 1452, 1834, 1992, 2143, 2333
PleI GAGTC 3 cut(s) 12, 736, 2755
PmaCI CACGTG 1 cut(s) 1370
PmlI CACGTG 1 cut(s) 1370
PpsI GAGTC 3 cut(s) 12, 736, 2755
Ppu21I YACGTR 1 cut(s) 1370
PpuMI RGGWCCY 1 cut(s) 2498
PscI ACATGT 1 cut(s) 2373
PshBI ATTAAT 1 cut(s) 237
Psp124BI GAGCTC 2 cut(s) 81, 2308
Psp1406I AACGTT 1 cut(s) 959
Psp5II RGGWCCY 1 cut(s) 2498
Psp6I CCWGG 5 cut(s) 193, 1453, 1702, 1726, 1768
PspCI CACGTG 1 cut(s) 1370
PspGI CCWGG 5 cut(s) 193, 1453, 1702, 1726, 1768
PspN4I GGNNCC 7 cut(s) 55, 110, 1347, 2173, 2499, 2500, 2944
PspPI GGNCC 3 cut(s) 410, 1346, 2498
PspPPI RGGWCCY 1 cut(s) 2498
PspXI VCTCGAGB 1 cut(s) 92
PstI CTGCAG 2 cut(s) 2143, 2314
PstNI CAGNNNCTG 1 cut(s) 2147
PsuI RGATCY 6 cut(s) 108, 385, 426, 521, 907, 1000
RsaI GTAC 3 cut(s) 864, 1468, 2348
RsaNI GTAC 3 cut(s) 863, 1467, 2347
RseI CAYNNNNRTG 1 cut(s) 1283
SacI GAGCTC 2 cut(s) 81, 2308
SapI GCTCTTC 1 cut(s) 1954
SaqAI TTAA 8 cut(s) 237, 582, 704, 962, 1017, 1169, 1482, 2456
SatI GCNGC 7 cut(s) 157, 515, 1451, 1833, 1991, 2142, 2332
Sau96I GGNCC 3 cut(s) 410, 1346, 2498
SchI GAGTC 3 cut(s) 13, 737, 2755
ScrFI CCNGG 8 cut(s) 195, 420, 1455, 1516, 1704, 1728, 1770, 2064
SduI GDGCHC 3 cut(s) 81, 2308, 2803
SfaNI GCATC 3 cut(s) 2153, 2569, 3043
SfcI CTRYAG 2 cut(s) 2139, 2310
Sfr274I CTCGAG 2 cut(s) 92, 752
SfuI TTCGAA 2 cut(s) 477, 1787
SinI GGWCC 2 cut(s) 410, 2498
SlaI CTCGAG 2 cut(s) 92, 752
SmiMI CAYNNNNRTG 1 cut(s) 1283
SmlI CTYRAG 7 cut(s) 92, 581, 752, 867, 1309, 1880, 2707
SmoI CTYRAG 7 cut(s) 92, 581, 752, 867, 1309, 1880, 2707
SseBI AGGCCT 1 cut(s) 3100
SsiI CCGC 6 cut(s) 797, 1403, 1685, 1742, 2034, 2777
SspI AATATT 1 cut(s) 828
SspMI CTAG 7 cut(s) 492, 899, 905, 1329, 1926, 2321, 2904
SstI GAGCTC 2 cut(s) 81, 2308
StuI AGGCCT 1 cut(s) 3100
StyD4I CCNGG 8 cut(s) 193, 418, 1453, 1514, 1702, 1726, 1768, 2062
StyI CCWWGG 1 cut(s) 2335
TaiI ACGT 4 cut(s) 962, 1068, 1372, 2271
TaqII GACCGA 1 cut(s) 2972
TatI WGTACW 1 cut(s) 2346
Tru1I TTAA 8 cut(s) 237, 582, 704, 962, 1017, 1169, 1482, 2456
Tru9I TTAA 8 cut(s) 237, 582, 704, 962, 1017, 1169, 1482, 2456
TscAI CASTG 9 cut(s) 169, 580, 727, 1035, 1276, 1290, 2349, 2364, 2645
TseFI GTSAC 4 cut(s) 64, 113, 677, 2918
TseI GCWGC 7 cut(s) 156, 514, 1450, 1832, 1990, 2141, 2331
Tsp45I GTSAC 4 cut(s) 64, 113, 677, 2918
TspDTI ATGAA 6 cut(s) 342, 942, 1267, 1683, 2645, 3108
TspGWI ACGGA 9 cut(s) 89, 89, 101, 423, 665, 996, 1747, 2466, 2923
TspRI CASTG 9 cut(s) 169, 580, 727, 1035, 1276, 1290, 2349, 2364, 2645
Van91I CCANNNNNTGG 1 cut(s) 1621
Vha464I CTTAAG 1 cut(s) 581
VneI GTGCAC 1 cut(s) 2799
VpaK11BI GGWCC 2 cut(s) 410, 2498
VspI ATTAAT 1 cut(s) 237
XagI CCTNNNNNAGG 1 cut(s) 1359
XapI RAATTY 5 cut(s) 272, 685, 1393, 1593, 2038
XceI RCATGY 1 cut(s) 2377
XhoI CTCGAG 2 cut(s) 92, 752
XmnI GAANNNNTTC 3 cut(s) 1052, 2423, 2517
XspI CTAG 7 cut(s) 492, 899, 905, 1329, 1926, 2321, 2904
Zsp2I ATGCAT 1 cut(s) 2562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.