Rw2G020370
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016140
Physical Location & Seq
Reverse (-)
27813446 .. 27819627
6182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G020370.1

Sequence Viewer

Length: 3201 bp
ATGATTTTTAGGGTTGTTGTTTCGGCTCCGATGAACAGTTTCACTCTCACTCTTCTCTACTGGTTCTTTGTTTTGGCGGGTTCCGCCTACTCGGCCACCGAGTCGGAGCTCCGATCCCTCTACGAGTTCAAGAAAGGGATCCAAATTGACCCGCTCCGGAAAGTCCTCGACACGTGGACCGACATCTCACTCAACCAAGCTCAGCCTTGCCCTCCCTGGACCGGCGTTTACTGCGACGACGGCGGCAATGTCGTCGCCGTGGCACTCGACGGCCTCGCCCTCGGCGGCGAGCTTAAGTTGAATACCCTCACTGGCCTCACTGCATTGCAAAACCTCAGTCTTGCCAACAATGACTTCACGGGTCGGGTCCCGCCCGCTCTGGGCGCAATGTCCTCTCTCCAGCACTTGGATCTGTCCGCGAACCGGTTCTACGGACCGATCCCGTCCCGGATTAACGACCTGTGGGGCTTGAATTATCTCAACTTGTCTCGGAATCTCTTCAAAGGTGGGTTCCCGGGCCCGGCGTCGAATCTCAATCAGCTCAAGGTGTTCGATGTGCATTACAATCAGCTGTGGGGGGACATTTCGGATTTGTTGCAGGAGTTTCATAATGTGGAGTACGTTGATTTGAGCTACAATGAGTTTTTCGGAGGGATTGAGCTGGGTTCTGAGAACATTTCGAGCTTATCCAACACTGTGCGTCACTTGAACTTCAGCCACAATAATCTGACTGGCCGGTTCTTTAAGGCTGATTCAGTCGGATTGTTTAGGAACTTGCGAGTTTTGGACTTGGGTGGGAATCAGATCACTGGGGAGCTCCCTTCATTTGGGTCGTTGCCGAATTTGCGGGTTTTGAGGCTGGCCAATAATCAGTTCTTTGGGGAAATACCGGAGGAGTTGCTACAGAGTTCTATTCCAATGGAGGAGTTGGATCTTAGTGGAAATGCTTTTACAGGTTCCATCACTGGAATTAACTCTACAACTTTGAAAGTCTTGAATCTGTCATCAAATGGTATGTCGGGAACATTGCAGAATGTGGATATGAGGAGTTGTGTTGTGGTAGATTTGAGCAGAAATAAGATTTCAGGAGATATATCAATGTTGGAGAATATGGGTACTGCTTTAGAGGTTCTCGATTTGAGTTCAAACAATTTTTCTGGAAGCTTAACAAACTTGAATATGCAGTTCGAGAGATTAACTACACTTAGTCTGAGAGATAATTTATTAGCAGGGTCTTTGCCTTCAATACTGAAGGCCTGCCCCAGACTCTCTACAGTTGACTTGAGTCAAAATGATTTCAGTGGGTCCATTCCTGGTAGTTTCTTCTCTTCACTGACATTGACAAGGTTGAATCTTTCACGAAATCACTTAAATGGACCAATTCCTCTCCAAGGCGGACATGTAAGCGAATTCTTAGCTCTTCCTCCAAATTTGCCAATTGAGAATGTCGATCTCTCCAATAATTCTTTATCGGGTTCCCTTCCTCGGGACATTGGTAACATGGTGGAACTCAAATTGCTAAACCTTGCAAAGAATGAGTTTTCTGGAGAACTACCTAGTGAATTGAGTAAACTTGCTAAGTTGAAGTACCTTGATTTATCAGGTAACAAATTCAAGGGCACAATTCCTGAGCAGCTTCCATCCAGCTTAATTGTATTTAATGTCTCCTATAACGATCTGTCTGGTTCTGTTCCGGACAATTTAAAAAGCTTCCCCATGACTTCATTTCGTCCTGGAAATGAGTTGCTAAACTTACCAAATGATGATCGGGCTAGATCTTCAGTTCCAGGTCGTATTCCAGAACAAGGAAAAAGTCATACTTCAAAGGCTCATATTAGGATAGCAATCATTGTTGCTTCAGTTGGAGTTACTTTGATGATAGTTTTTGTTTTGTTGGTTTATCATCAGACACACCATAAAGGATTTCATGGAAGAAATGGATTTGGCGGCGAAAATACAGGGACAGATGTTAAGATTGGCAGATTCACACGTCCTTCATTTTTGAATTTCCAAACGAATGTTCAGCCTCCACCAACTTCATTGAGCTTTTCAAATGATCACTTGCTAACTTCACAATCCAGGTCATTGTCAGGGCAGGCAGAGTTTGTACCAGAAATTGGGAAGCCTGTTTTACCTGGGGAAGCAGCAACGAGTTCTACACCTATGAATCTTCTTGATAACCAGCCTACAACTTCTGGAAGGAAGTCCTCCCCTGGTTCTCCACTATCTTCCTCACCTCGTTTTATAGAGGCATATGAGCAACCTGTAATACTGGATGTGTACTCACCAGATCGGTTGGCTGGAGAACTGTTCTTCCTAGATGCTTCATTGCAATTTACAGCTGAGGAATTATCTCGAGCTCCTGCAGAAGTTCTGGGCAGAAGCAGCCATGGAACTCTGTACAAAGCTACTCTAGATAGTGGGCACATGTTGACGGTGAAATGGTTACGGGTGGGACTGGTCAAGCATAAGAAAGATTTTGCCAAGGAAGTCAAAAGGATTGGCTCTATAAGGCATCCAAACATTGTTCCATTGCAGGCATACTATTGGGGGCCTAGGGAACAAGAGAGGCTTCTTTTAGCAGACTATGTTCAGGGAGATAGCTTGGCCCTACATCTTTATGAGAGCACGCCTCGGAGGTACTCCCCGTTATCATTCAACCAGAGGCTAAAAGTAGCTGTGGAGGTTGCTCGATGTTTGCTATACCTACATGATAGAGGCCTGCCCCATGGAAACCTAAAGCCAACTAATGTAATCCTGGCAGGTCCTGAGTACCATCCTCGGCTTACTGATTACAGTCTTCACCGTCTAATGACACCAGCTGGCATTGCGGAGCAGTTTTTAAATATGGGAGCTCTTGGATACCGTGCTCCAGAACTTGCTACTGCAGCAAAACCCGTCCCTTCTTTCAAAGCTGACGTTTATGCCTTTGGAGTGATTTTGATGGAAATGTTAACCAGAAGAAGTGCTGGTGACATTATATCGGGTCAGTCAGGGGCGGTTGACCTTACAGACTGGGTTCGGCTTTGTGATCAAGAAGGACGAGGAATGGATTGCATTGACAGAGACATTGCTGGTGGAGAAGAACCCTCAAAAGCAATGGATGAATTGCTTGCAATATCACTGAGGTGTATTCTCCCTGTAAATGAGAGGCCTAACATCAGACAAGTATTTGACAACCTCTGTTCTATATCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1066

Amino Acids

116.86

Weight (kDa)

6.12

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 35 - 79 9.6e-06 Leucine rich repeat N-terminal domain
LRR_4 PF12799 258 - 292 1.7e-08 Leucine Rich repeats (2 copies)
LRR_8 PF13855 481 - 538 5.2e-06 Leucine rich repeat
Pkinase PF00069 790 - 992 1.6e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 790 - 987 2.5e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10020 AT5G10020
fragaria_vesca FvH4_1g22060
malus_domestica MD01G1018200.v1.1
pyrus_communis pycom01g04790
rosa_chinensis RchiOBHm_Chr2g0116131
rosa_laevigata RLG00000018242
rosa_multiflora Rmu_sc0002288.1_g000016
rosa_roxburghii Rroxscaffold_2G00127470
rosa_rugosa Rorug02G0203700
rosa_samantha Rh2AG260100 Rh2BG271100 Rh2CG266400 Rh2DG268300
rosa_wichuraiana Rw2G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2753
AccB7I CCANNNNNTGG 2 cut(s) 406, 2117
AccBSI CCGCTC 2 cut(s) 154, 377
AccII CGCG 1 cut(s) 419
AccIII TCCGGA 2 cut(s) 156, 1693
AclWI GGATC 6 cut(s) 108, 133, 146, 417, 433, 939
AcoI YGGCCR 3 cut(s) 93, 733, 861
AcsI RAATTY 5 cut(s) 841, 1409, 1429, 1610, 2005
AcuI CTGAAG 4 cut(s) 697, 1271, 1764, 1842
AcvI CACGTG 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 524
AfaI GTAC 8 cut(s) 620, 1117, 1589, 2109, 2282, 2402, 2642, 2774
AflII CTTAAG 1 cut(s) 293
AflIII ACRYGT 4 cut(s) 171, 1399, 1988, 2427
AgeI ACCGGT 1 cut(s) 423
AjiI CACGTC 1 cut(s) 1991
AjnI CCWGG 8 cut(s) 215, 1312, 1732, 1786, 2078, 2134, 2212, 2757
AjuI GAANNNNNNNTTGG 6 cut(s) 338, 370, 857, 889, 2004, 2036
AleI CACNNNNGTG 1 cut(s) 3127
AloI GAACNNNNNNTCC 2 cut(s) 2298, 2330
Alw21I GWGCWC 6 cut(s) 111, 819, 2362, 2630, 2857, 2872
Alw26I GTCTC 3 cut(s) 492, 1669, 3060
AlwI GGATC 6 cut(s) 108, 133, 146, 417, 433, 939
AlwNI CAGNNNCTG 1 cut(s) 2768
Ama87I CYCGRG 3 cut(s) 514, 1485, 2355
Aor13HI TCCGGA 2 cut(s) 156, 1693
ApaI GGGCCC 1 cut(s) 521
ApeKI GCWGC 4 cut(s) 1633, 2144, 2385, 2888
ApoI RAATTY 5 cut(s) 841, 1409, 1429, 1610, 2005
ArsI GACNNNNNNTTYG 4 cut(s) 322, 354, 1001, 1033
AsiGI ACCGGT 1 cut(s) 423
Asp700I GAANNNNTTC 3 cut(s) 38, 425, 497
AspA2I CCTAGG 1 cut(s) 2555
AspLEI GCGC 1 cut(s) 386
AsuC2I CCSGG 4 cut(s) 448, 515, 516, 521
AsuHPI GGTGA 5 cut(s) 2226, 2277, 2449, 2795, 2984
AvaI CYCGRG 3 cut(s) 514, 1485, 2355
AvaII GGWCC 7 cut(s) 177, 219, 367, 434, 1305, 1376, 2765
AvrII CCTAGG 1 cut(s) 2555
BaeGI GKGCMC 3 cut(s) 521, 1622, 2427
BalI TGGCCA 1 cut(s) 863
BamHI GGATCC 1 cut(s) 138
BanII GRGCYC 5 cut(s) 111, 521, 819, 2362, 2857
BbrPI CACGTG 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 2792
Bbv12I GWGCWC 6 cut(s) 111, 819, 2362, 2630, 2857, 2872
BbvCI CCTCAGC 1 cut(s) 2343
BbvI GCAGC 4 cut(s) 1645, 2156, 2397, 2900
BccI CCATC 4 cut(s) 968, 1648, 2784, 2938
BceAI ACGGC 3 cut(s) 242, 256, 286
BcgI CGANNNNNNTGC 2 cut(s) 235, 269
BciT130I CCWGG 8 cut(s) 217, 1314, 1734, 1788, 2080, 2136, 2214, 2759
BciVI GTATCC 1 cut(s) 2855
BclI TGATCA 2 cut(s) 2056, 3031
BcnI CCSGG 4 cut(s) 448, 515, 516, 521
BcoDI GTCTC 3 cut(s) 492, 1669, 3060
BfaI CTAG 5 cut(s) 1557, 1773, 2318, 2414, 2556
BfmI CTRYAG 4 cut(s) 902, 1272, 2364, 2886
BfrI CTTAAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 2753
BfuI GTATCC 1 cut(s) 2855
BglI GCCNNNNNGGC 1 cut(s) 92
BglII AGATCT 1 cut(s) 1775
BisI GCNGC 7 cut(s) 244, 286, 1634, 1948, 2145, 2386, 2889
BlnI CCTAGG 1 cut(s) 2555
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 7 cut(s) 245, 287, 1635, 1949, 2146, 2387, 2890
Bme18I GGWCC 7 cut(s) 177, 219, 367, 434, 1305, 1376, 2765
BmeT110I CYCGRG 3 cut(s) 514, 1485, 2355
BmgBI CACGTC 1 cut(s) 1991
BmrI ACTGGG 2 cut(s) 819, 3025
BmsI GCATC 2 cut(s) 2311, 2524
BmuI ACTGGG 2 cut(s) 819, 3025
BoxI GACNNNNGTC 1 cut(s) 1284
BpiI GAAGAC 1 cut(s) 2792
BplI GAGNNNNNCTC 2 cut(s) 2617, 2649
BpmI CTGGAG 4 cut(s) 383, 1566, 2322, 2856
Bpu10I CCTNAGC 2 cut(s) 1629, 2343
Bpu1102I GCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 527, 1303
BpuMI CCSGG 4 cut(s) 448, 515, 516, 521
BsaAI YACGTR 1 cut(s) 174
BsaBI GATNNNNATC 1 cut(s) 1844
BsaHI GRCGYC 1 cut(s) 524
BsaWI WCCGGW 4 cut(s) 156, 423, 889, 1693
Bse118I RCCGGY 3 cut(s) 221, 423, 735
Bse1I ACTGG 8 cut(s) 65, 316, 736, 814, 970, 2277, 2463, 3020
Bse3DI GCAATG 9 cut(s) 253, 323, 393, 1025, 2327, 2531, 2826, 3069, 3105
Bse8I GATNNNNATC 1 cut(s) 1844
BseAI TCCGGA 2 cut(s) 156, 1693
BseBI CCWGG 8 cut(s) 217, 1314, 1734, 1788, 2080, 2136, 2214, 2759
BseGI GGATG 5 cut(s) 1640, 2281, 2515, 2776, 3109
BseJI GATNNNNATC 1 cut(s) 1844
BseMI GCAATG 9 cut(s) 253, 323, 393, 1025, 2327, 2531, 2826, 3069, 3105
BseMII CTCAG 8 cut(s) 215, 349, 660, 1202, 1620, 2334, 2760, 3116
BseNI ACTGG 8 cut(s) 65, 316, 736, 814, 970, 2277, 2463, 3020
BseRI GAGGAG 3 cut(s) 908, 938, 1060
BseSI GKGCMC 3 cut(s) 521, 1622, 2427
BseXI GCAGC 4 cut(s) 1645, 2156, 2397, 2900
BseYI CCCAGC 1 cut(s) 661
Bsh1236I CGCG 1 cut(s) 419
BshTI ACCGGT 1 cut(s) 423
BsiHKAI GWGCWC 6 cut(s) 111, 819, 2362, 2630, 2857, 2872
BsiHKCI CYCGRG 3 cut(s) 514, 1485, 2355
BsiSI CCGG 9 cut(s) 157, 222, 424, 448, 515, 521, 736, 890, 1694
BslFI GGGAC 7 cut(s) 353, 430, 593, 1502, 1975, 2469, 2885
BsmAI GTCTC 3 cut(s) 492, 1669, 3060
BsmFI GGGAC 7 cut(s) 353, 430, 593, 1502, 1975, 2469, 2885
BsoBI CYCGRG 3 cut(s) 514, 1485, 2355
Bsp120I GGGCCC 1 cut(s) 517
Bsp1286I GDGCHC 9 cut(s) 111, 521, 819, 1622, 2362, 2427, 2630, 2857, 2872
Bsp13I TCCGGA 2 cut(s) 156, 1693
Bsp1407I TGTACA 1 cut(s) 2400
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp19I CCATGG 2 cut(s) 2389, 2728
BspCNI CTCAG 8 cut(s) 214, 348, 661, 1203, 1621, 2335, 2761, 3117
BspEI TCCGGA 2 cut(s) 156, 1693
BspFNI CGCG 1 cut(s) 419
BspMAI CTGCAG 2 cut(s) 2368, 2890
BspMI ACCTGC 1 cut(s) 2753
BspPI GGATC 6 cut(s) 108, 133, 146, 417, 433, 939
BspQI GCTCTTC 1 cut(s) 1425
BspTI CTTAAG 1 cut(s) 293
BsrBI CCGCTC 2 cut(s) 154, 377
BsrDI GCAATG 9 cut(s) 253, 323, 393, 1025, 2327, 2531, 2826, 3069, 3105
BsrFI RCCGGY 3 cut(s) 221, 423, 735
BsrGI TGTACA 1 cut(s) 2400
BsrI ACTGG 8 cut(s) 65, 316, 736, 814, 970, 2277, 2463, 3020
BssAI RCCGGY 3 cut(s) 221, 423, 735
BssNI GRCGYC 1 cut(s) 524
BssT1I CCWWGG 5 cut(s) 1390, 2389, 2484, 2555, 2728
Bst2UI CCWGG 8 cut(s) 217, 1314, 1734, 1788, 2080, 2136, 2214, 2759
Bst4CI ACNGT 8 cut(s) 38, 697, 1276, 2310, 2437, 2798, 2807, 2867
Bst6I CTCTTC 4 cut(s) 57, 503, 1333, 1425
BstACI GRCGYC 1 cut(s) 524
BstAFI CTTAAG 1 cut(s) 293
BstAUI TGTACA 1 cut(s) 2400
BstBAI YACGTR 1 cut(s) 174
BstDSI CCRYGG 3 cut(s) 258, 2389, 2728
BstENI CCTNNNNNAGG 1 cut(s) 1389
BstF5I GGATG 5 cut(s) 1640, 2281, 2515, 2776, 3109
BstFNI CGCG 1 cut(s) 419
BstHHI GCGC 1 cut(s) 386
BstMAI GTCTC 3 cut(s) 492, 1669, 3060
BstMWI GCNNNNNNNGC 9 cut(s) 83, 92, 231, 240, 383, 844, 2385, 2828, 2888
BstNI CCWGG 8 cut(s) 217, 1314, 1734, 1788, 2080, 2136, 2214, 2759
BstNSI RCATGY 2 cut(s) 1403, 2431
BstPAI GACNNNNGTC 1 cut(s) 1284
BstSFI CTRYAG 4 cut(s) 902, 1272, 2364, 2886
BstSLI GKGCMC 3 cut(s) 521, 1622, 2427
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 4 cut(s) 1645, 2156, 2397, 2900
BstV2I GAAGAC 1 cut(s) 2792
BstX2I RGATCY 4 cut(s) 138, 409, 931, 1775
BstYI RGATCY 4 cut(s) 138, 409, 931, 1775
BsuI GTATCC 1 cut(s) 2855
BtgI CCRYGG 3 cut(s) 258, 2389, 2728
BtrI CACGTC 1 cut(s) 1991
BtsCI GGATG 5 cut(s) 1640, 2281, 2515, 2776, 3109
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 8 cut(s) 309, 318, 693, 807, 963, 1306, 1331, 3122
BveI ACCTGC 1 cut(s) 2753
CaiI CAGNNNCTG 1 cut(s) 2768
CfoI GCGC 1 cut(s) 386
Cfr10I RCCGGY 3 cut(s) 221, 423, 735
Cfr9I CCCGGG 1 cut(s) 514
CpoI CGGWCCG 1 cut(s) 434
CseI GACGC 2 cut(s) 513, 689
Csp6I GTAC 8 cut(s) 619, 1116, 1588, 2108, 2281, 2401, 2641, 2773
CspAI ACCGGT 1 cut(s) 423
CspI CGGWCCG 1 cut(s) 434
CviAII CATG 8 cut(s) 1400, 1501, 1717, 1928, 2390, 2428, 2711, 2729
CviQI GTAC 8 cut(s) 619, 1116, 1588, 2108, 2281, 2401, 2641, 2773
DraI TTTAAA 2 cut(s) 1704, 2844
EaeI YGGCCR 3 cut(s) 93, 733, 861
Eam1104I CTCTTC 4 cut(s) 57, 503, 1333, 1425
EarI CTCTTC 4 cut(s) 57, 503, 1333, 1425
EciI GGCGGA 2 cut(s) 73, 1410
Ecl136II GAGCTC 4 cut(s) 109, 817, 2360, 2855
Eco130I CCWWGG 5 cut(s) 1390, 2389, 2484, 2555, 2728
Eco147I AGGCCT 3 cut(s) 1256, 2721, 3154
Eco24I GRGCYC 5 cut(s) 111, 521, 819, 2362, 2857
Eco47I GGWCC 7 cut(s) 177, 219, 367, 434, 1305, 1376, 2765
Eco53kI GAGCTC 4 cut(s) 109, 817, 2360, 2855
Eco57I CTGAAG 4 cut(s) 697, 1271, 1764, 1842
Eco72I CACGTG 1 cut(s) 174
Eco88I CYCGRG 3 cut(s) 514, 1485, 2355
EcoICRI GAGCTC 4 cut(s) 109, 817, 2360, 2855
EcoNI CCTNNNNNAGG 1 cut(s) 1389
EcoO109I RGGNCCY 3 cut(s) 367, 2552, 2765
EcoRI GAATTC 1 cut(s) 1409
EcoRII CCWGG 8 cut(s) 215, 1312, 1732, 1786, 2078, 2134, 2212, 2757
EcoT14I CCWWGG 5 cut(s) 1390, 2389, 2484, 2555, 2728
EcoT38I GRGCYC 5 cut(s) 111, 521, 819, 2362, 2857
ErhI CCWWGG 5 cut(s) 1390, 2389, 2484, 2555, 2728
FaeI CATG 8 cut(s) 1403, 1504, 1720, 1931, 2393, 2431, 2714, 2732
FalI AAGNNNNNCTT 4 cut(s) 1804, 1836, 2556, 2588
FaqI GGGAC 7 cut(s) 353, 430, 593, 1502, 1975, 2469, 2885
FatI CATG 8 cut(s) 1399, 1500, 1716, 1927, 2389, 2427, 2710, 2728
FauI CCCGC 5 cut(s) 70, 159, 378, 382, 840
FauNDI CATATG 1 cut(s) 2254
FbaI TGATCA 2 cut(s) 2056, 3031
Fnu4HI GCNGC 7 cut(s) 244, 286, 1634, 1948, 2145, 2386, 2889
FokI GGATG 5 cut(s) 1627, 2288, 2502, 2763, 3116
FriOI GRGCYC 5 cut(s) 111, 521, 819, 2362, 2857
Fsp4HI GCNGC 7 cut(s) 244, 286, 1634, 1948, 2145, 2386, 2889
FspBI CTAG 5 cut(s) 1557, 1773, 2318, 2414, 2556
GlaI GCGC 1 cut(s) 385
GluI GCNGC 7 cut(s) 244, 286, 1634, 1948, 2145, 2386, 2889
GsaI CCCAGC 1 cut(s) 665
GsuI CTGGAG 4 cut(s) 383, 1566, 2322, 2856
HapII CCGG 9 cut(s) 157, 222, 424, 448, 515, 521, 736, 890, 1694
HgaI GACGC 2 cut(s) 513, 689
HhaI GCGC 1 cut(s) 386
Hin1I GRCGYC 1 cut(s) 524
Hin1II CATG 8 cut(s) 1403, 1504, 1720, 1931, 2393, 2431, 2714, 2732
Hin6I GCGC 1 cut(s) 384
HinP1I GCGC 1 cut(s) 384
HincII GTYRAC 4 cut(s) 1279, 2433, 2955, 3004
HindII GTYRAC 4 cut(s) 1279, 2433, 2955, 3004
HindIII AAGCTT 2 cut(s) 1162, 1708
HpaI GTTAAC 1 cut(s) 2955
HpaII CCGG 9 cut(s) 157, 222, 424, 448, 515, 521, 736, 890, 1694
HphI GGTGA 5 cut(s) 2226, 2277, 2449, 2795, 2984
Hpy166II GTNNAC 8 cut(s) 177, 229, 1279, 1571, 2281, 2433, 2955, 3004
Hpy8I GTNNAC 8 cut(s) 177, 229, 1279, 1571, 2281, 2433, 2955, 3004
Hpy99I CGWCG 5 cut(s) 239, 242, 257, 272, 529
HpyAV CCTTC 8 cut(s) 831, 1246, 1251, 1490, 2004, 2193, 2913, 3032
HpyCH4III ACNGT 8 cut(s) 38, 697, 1276, 2310, 2437, 2798, 2807, 2867
HpyCH4IV ACGT 4 cut(s) 173, 621, 1990, 2919
HpyF10VI GCNNNNNNNGC 9 cut(s) 83, 92, 231, 240, 383, 844, 2385, 2828, 2888
HpySE526I ACGT 4 cut(s) 173, 621, 1990, 2919
Hsp92I GRCGYC 1 cut(s) 524
Hsp92II CATG 8 cut(s) 1403, 1504, 1720, 1931, 2393, 2431, 2714, 2732
HspAI GCGC 1 cut(s) 384
KflI GGGWCCC 1 cut(s) 367
Kpn2I TCCGGA 2 cut(s) 156, 1693
Ksp22I TGATCA 2 cut(s) 2056, 3031
KspAI GTTAAC 1 cut(s) 2955
LguI GCTCTTC 1 cut(s) 1425
Lsp1109I GCAGC 4 cut(s) 1645, 2156, 2397, 2900
LweI GCATC 2 cut(s) 2311, 2524
MaeI CTAG 5 cut(s) 1557, 1773, 2318, 2414, 2556
MaeII ACGT 4 cut(s) 173, 621, 1990, 2919
MaeIII GTNAC 6 cut(s) 701, 1496, 1604, 1867, 2445, 2972
MbiI CCGCTC 2 cut(s) 154, 377
MfeI CAATTG 1 cut(s) 1437
MflI RGATCY 4 cut(s) 138, 409, 931, 1775
MhlI GDGCHC 9 cut(s) 111, 521, 819, 1622, 2362, 2427, 2630, 2857, 2872
MlsI TGGCCA 1 cut(s) 863
MluNI TGGCCA 1 cut(s) 863
MlyI GAGTC 3 cut(s) 110, 1260, 1294
MmeI TCCRAC 6 cut(s) 84, 714, 739, 909, 1083, 1843
Mox20I TGGCCA 1 cut(s) 863
MroI TCCGGA 2 cut(s) 156, 1693
MroXI GAANNNNTTC 3 cut(s) 38, 425, 497
MscI TGGCCA 1 cut(s) 863
MslI CAYNNNNRTG 3 cut(s) 1371, 2619, 3127
Msp20I TGGCCA 1 cut(s) 863
MspA1I CMGCKG 3 cut(s) 571, 2342, 2822
MspCI CTTAAG 1 cut(s) 293
MspI CCGG 9 cut(s) 157, 222, 424, 448, 515, 521, 736, 890, 1694
MunI CAATTG 1 cut(s) 1437
MvaI CCWGG 8 cut(s) 217, 1314, 1734, 1788, 2080, 2136, 2214, 2759
MvnI CGCG 1 cut(s) 419
MwoI GCNNNNNNNGC 9 cut(s) 83, 92, 231, 240, 383, 844, 2385, 2828, 2888
NciI CCSGG 4 cut(s) 448, 515, 516, 521
NcoI CCATGG 2 cut(s) 2389, 2728
NdeI CATATG 1 cut(s) 2254
NlaIII CATG 8 cut(s) 1403, 1504, 1720, 1931, 2393, 2431, 2714, 2732
NmeAIII GCCGAG 3 cut(s) 71, 261, 2761
NmuCI GTSAC 2 cut(s) 701, 2972
NspI RCATGY 2 cut(s) 1403, 2431
OliI CACNNNNGTG 1 cut(s) 3127
PaeR7I CTCGAG 1 cut(s) 2355
PceI AGGCCT 3 cut(s) 1256, 2721, 3154
PciI ACATGT 2 cut(s) 1399, 2427
PciSI GCTCTTC 1 cut(s) 1425
PdmI GAANNNNTTC 3 cut(s) 38, 425, 497
PfeI GAWTC 8 cut(s) 493, 529, 752, 799, 997, 1351, 1983, 2167
PflMI CCANNNNNTGG 2 cut(s) 406, 2117
PfoI TCCNGGA 2 cut(s) 446, 1732
PinAI ACCGGT 1 cut(s) 423
PkrI GCNGC 7 cut(s) 245, 287, 1635, 1949, 2146, 2387, 2890
PleI GAGTC 3 cut(s) 109, 1260, 1293
PmaCI CACGTG 1 cut(s) 174
PmlI CACGTG 1 cut(s) 174
PpsI GAGTC 3 cut(s) 109, 1260, 1293
Ppu21I YACGTR 1 cut(s) 174
PpuMI RGGWCCY 2 cut(s) 367, 2765
PscI ACATGT 2 cut(s) 1399, 2427
PshAI GACNNNNGTC 1 cut(s) 1284
Psp124BI GAGCTC 4 cut(s) 111, 819, 2362, 2857
Psp5II RGGWCCY 2 cut(s) 367, 2765
Psp6I CCWGG 8 cut(s) 215, 1312, 1732, 1786, 2078, 2134, 2212, 2757
PspCI CACGTG 1 cut(s) 174
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 8 cut(s) 215, 1312, 1732, 1786, 2078, 2134, 2212, 2757
PspOMI GGGCCC 1 cut(s) 517
PspPPI RGGWCCY 2 cut(s) 367, 2765
PsrI GAACNNNNNNTAC 2 cut(s) 413, 445
PstI CTGCAG 2 cut(s) 2368, 2890
PstNI CAGNNNCTG 1 cut(s) 2768
PsuI RGATCY 4 cut(s) 138, 409, 931, 1775
PvuII CAGCTG 3 cut(s) 571, 2342, 2822
RsaI GTAC 8 cut(s) 620, 1117, 1589, 2109, 2282, 2402, 2642, 2774
RsaNI GTAC 8 cut(s) 619, 1116, 1588, 2108, 2281, 2401, 2641, 2773
RseI CAYNNNNRTG 3 cut(s) 1371, 2619, 3127
Rsr2I CGGWCCG 1 cut(s) 434
RsrII CGGWCCG 1 cut(s) 434
SacI GAGCTC 4 cut(s) 111, 819, 2362, 2857
SapI GCTCTTC 1 cut(s) 1425
SatI GCNGC 7 cut(s) 244, 286, 1634, 1948, 2145, 2386, 2889
SchI GAGTC 3 cut(s) 110, 1260, 1294
SduI GDGCHC 9 cut(s) 111, 521, 819, 1622, 2362, 2427, 2630, 2857, 2872
SfaNI GCATC 2 cut(s) 2311, 2524
SfcI CTRYAG 4 cut(s) 902, 1272, 2364, 2886
Sfr274I CTCGAG 1 cut(s) 2355
SinI GGWCC 7 cut(s) 177, 219, 367, 434, 1305, 1376, 2765
SlaI CTCGAG 1 cut(s) 2355
SmaI CCCGGG 1 cut(s) 516
SmiMI CAYNNNNRTG 3 cut(s) 1371, 2619, 3127
SmlI CTYRAG 4 cut(s) 293, 542, 1282, 2355
SmoI CTYRAG 4 cut(s) 293, 542, 1282, 2355
SseBI AGGCCT 3 cut(s) 1256, 2721, 3154
SspMI CTAG 5 cut(s) 1557, 1773, 2318, 2414, 2556
SstI GAGCTC 4 cut(s) 111, 819, 2362, 2857
StuI AGGCCT 3 cut(s) 1256, 2721, 3154
StyI CCWWGG 5 cut(s) 1390, 2389, 2484, 2555, 2728
TaaI ACNGT 8 cut(s) 38, 697, 1276, 2310, 2437, 2798, 2807, 2867
TaiI ACGT 4 cut(s) 176, 624, 1993, 2922
TaqII GACCGA 2 cut(s) 194, 451
TatI WGTACW 2 cut(s) 2280, 2400
TauI GCSGC 3 cut(s) 246, 288, 1950
TfiI GAWTC 8 cut(s) 493, 529, 752, 799, 997, 1351, 1983, 2167
TscAI CASTG 8 cut(s) 316, 325, 700, 814, 970, 1306, 1338, 3129
TseFI GTSAC 2 cut(s) 701, 2972
TseI GCWGC 4 cut(s) 1633, 2144, 2385, 2888
Tsp45I GTSAC 2 cut(s) 701, 2972
TspGWI ACGGA 1 cut(s) 447
TspMI CCCGGG 1 cut(s) 514
TspRI CASTG 8 cut(s) 316, 325, 700, 814, 970, 1306, 1338, 3129
Van91I CCANNNNNTGG 2 cut(s) 406, 2117
Vha464I CTTAAG 1 cut(s) 293
VpaK11BI GGWCC 7 cut(s) 177, 219, 367, 434, 1305, 1376, 2765
XagI CCTNNNNNAGG 1 cut(s) 1389
XapI RAATTY 5 cut(s) 841, 1409, 1429, 1610, 2005
XbaI TCTAGA 1 cut(s) 2413
XceI RCATGY 2 cut(s) 1403, 2431
XhoI CTCGAG 1 cut(s) 2355
XmaI CCCGGG 1 cut(s) 514
XmaJI CCTAGG 1 cut(s) 2555
XmnI GAANNNNTTC 3 cut(s) 38, 425, 497
XspI CTAG 5 cut(s) 1557, 1773, 2318, 2414, 2556
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.