RLG00000018242
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016140
Physical Location & Seq
Reverse (-)
27474752 .. 27480389
5638 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018242

Sequence Viewer

Length: 3177 bp
ATGAACAGTTTCACTCTCACTCTCACTCTTCTCTACTTGTTCTTTGTTTTGGCGGGTTCCGCCTACTCGGCCACCGAGTCGGAGCTCCGATCCCTCTACGAGTTCAAGAAAGGGATCCAAATTGACCCGCTCCGGAAAGTCCTCGACACATGGACCGACATCTCACTCAACCAAGCTCAGCCTTGCCCTCCCTGGACCGGCGTTTACTGCAACGACGGCGGCAATGTCGTCGCCGTAACACTCGACGGCCTCGGCCTCGGCGGCGAGCTTAAGTTGAATACCCTCACTGGCCTCACTGCATTGCAAAACCTCAGTCTTGCCAACAATGACTTCACGGGTCGGGTCCCACCCGCTCTGGGCGCAATGTCCTCTCTCCAGCACTTGGATCTGTCCGCGAACAGGTTCTACGGCCCGATCCCGTCCCGGATTAACGATTTGTGGGGCTTGAATTATCTCAACTTGTCTCGGAATCTCTTCAAAGGCGGGTTCCCGGGTCCGGCGTCGAATCTCAATCAGCTCAAAGTGTTCGATGTGCATTACAATCAGCTCTGGGGGGACATTTCCGATTTGTTGCAGGAGTTTCATAATGTGGAGTATGTTGATTTGAGCTGCAATGAGTTTTTCGGAGGGATTGAGCTGGGTTCTGAGAACGTTTCGAGCTTATCCAACACCGTGCGTCACTTGAACTTCAGCCACAATAATCTGACTGGCCGGTTCTTTAAGCCTGATTCATTTGGGTTGTTTAGGAACTTGCAAGTTTTGGACTTGGGTGGGAATCAGATCACTGGGGAGCTTCCTTCATTTGGGTCGTTGCCGAACTTGCGAGTTTTGAGGCTGGCGAATAATCAGTTCTTTGGGAAAATACCGGAGGAGTTGCTACAGAGTTCTATTCCAATGGAGGAGTTGGATCTTAGTGGAAATGCTTTTACAGGTTCCATCACTGTAATTAACTCTACAACTTTGAAAGTCTTGAATCTGTCATCAAATGGTATGTCGGGCACATTGCAGAATGTGGATATGAGGAGTTGTGTTGTGGTAGATTTGAGCAGAAATAAGATTTCGGGTGATATATCAATGTTGGAGAATATGGGTACTGCTTTAGAGGTTCTCGATTTGAGTTCAAACAATTTTTCTGGAAGCTTAACAAACTTGACTATGCAGTTCGAGAGATTAACTACACTTAGTCTGAGAGATAATTTGTTAGCGGGGTCTTTGCCTTCGATACTGAAGGCCTGCCCCAGACTCTCTACAGTTGACTTGAGTCAAAATGATTTCAGTGGGTCCATTCCTGGTAGTTTCTTCTCTTCACTGACATTGACAAGGTTGAATCTTTCACGAAATCACTTAAATGGGCCAATTCCTCTCCAAGGCGGACATGTAAGTGAATTCTTAGCTCTTCCTCCAGATTTGCCAATTGAGAATGTCGATCTCTCCAATAATTCTTTATCGGGTTCCCTTCCTCGGGACATTGGTAACATGGTGGAACTCAAATTGCTAAACCTTGCAAAGAATGAGTTTTCAGGAGAACTACCTAGTGAATTGAGTAAACTTGCTAAGTTGAAGTACCTTGATTTATCAGGTAACAAATTCAAGGGCGCAATTCCTGAGCAGCTTCCATCCAGCTTAATTGTATTTAATGTCTCCTATAACGATCTATCTGGTTCTGTTCCGGACAATTTAAAAAGCTTCCCCATGACTTCATTTCGTCCTGGAAATGAGTTGCTAAACTTACCAAATGATGATCGGGCTAGATCTTCAGTTCCAGGTCGTATTCCAGAACAAGGAAAAAGTCATACTTCGAAGGCTCATATTAGGATAGCAATCATTGTTGCTTCAGTTGGAGTTACTTTGATGATAGTTTTTGTTTTGTTGGTTTATCATCAGACACACCATAAAGGATTTCATGGAAGAAATGGATTTGGCGGCGAAAATACAGGGACAGATGTTAAGATTGGCAGATTCACACGTCCTTCATTTTTGAATTTCCAAACGAATGTTCAGCCTCCACCAACTTCATTGAGCTTTTCAAATGATCACTTGCTAACTCCACAATCCAGGTCATTGTCAGGGCAGGCAGAGTTTGTACCAGAACTTGGGAAGCCTGTTTTACCTGGGGAAGCAGCAACAAGTTCTACACCTATGAATCTTCTTGATAACCAGCCTACAACTTCTGGAAGGAAGTCCTCCCCTGGTTCTCCACTATCTTCCTCACCTCGTTTTATAGAGGCATATGAGCAACCTGTAATACTGGATGTGTACTCACCAGATCGGTTGGCTGGAGAACTGTTCTTCCTAGATGCTTCATTGCAATTTACAGCTGAGGAATTATCTCGAGCTCCTGCAGAAGTTCTAGGCAGAAGCAGCCATGGAACTCTGTACAAAGCTACTCTAGACAGTGGGCACATGTTGACGGTGAAATGGTTGCGGGTGGGACTGGTCAAGCATAAGAAAGATTTTGCCAAGGAAGTCAAAAGGATTGGCTCTATAAGGCATCCAAACATTGTTCCATTACAGGCATACTATTGGGGGCCCAGGGAACAAGAGAGGCTTCTTTTAGCGGACTATGTTCAGGGAGATAGCTTGGCCCTACATCTTTATGAGAGCACGCCTCGGAGGTACTCCCCGTTATCATTCAACCAGAGGCTAAAAGTAGCTGTGGAGGTTGCTCGATGTTTGCTATACCTACATGATAGAGGCCTGCCCCATGGGAACCTAAAGCCAACTAATGTAATCCTGGCAGGTCCTGAATACCATCCTCGGCTTACTGATTACAGTCTTCACCGTCTAATGACAGCAGCTGGCATTGCGGAGCAGTTTTTAAATATGGGAGCTCTTGGATACCGTGCTCCAGAACTTGCTACTGCAGCCAAACCAGTCCCTTCTTTCAAAGCTGACGTTTATGCCTTTGGAGTGATTTTGATGGAAATGTTAACCAGAAGAAGTGCTGGTGACATTATATCGGGTCAGTCAGGGGCGGTTGACCTTACAGACTGGGTTCGGCTTTGTGATCAAGAAGGACGAGGAATGGATTGCATCGACAGAGACATTGCTGGTGGAGAAGAGCCCTCAAAAGCAATGGATGAATTGCTTGCAATATCACTGAGGTGTATTCTCCCTGTAAATGAGAGGCCTAACATCAGACAAGTATTTGACAACCTCTGTTCTATATCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1059

Amino Acids

115.88

Weight (kDa)

6.12

Isoelectric Point (pI)

36.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 250 - 288 1.4e-08 Leucine Rich repeats (2 copies)
LRR_8 PF13855 473 - 530 5.2e-06 Leucine rich repeat
Pkinase PF00069 782 - 984 1.7e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 782 - 979 1.3e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10020 AT5G10020
fragaria_vesca FvH4_1g22060
malus_domestica MD01G1018200.v1.1
pyrus_communis pycom01g04790
rosa_chinensis RchiOBHm_Chr2g0116131
rosa_laevigata RLG00000018242
rosa_multiflora Rmu_sc0002288.1_g000016
rosa_roxburghii Rroxscaffold_2G00127470
rosa_rugosa Rorug02G0203700
rosa_samantha Rh2AG260100 Rh2BG271100 Rh2CG266400 Rh2DG268300
rosa_wichuraiana Rw2G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2729
AccB7I CCANNNNNTGG 2 cut(s) 382, 2093
AccBSI CCGCTC 2 cut(s) 130, 353
AccII CGCG 1 cut(s) 395
AccIII TCCGGA 2 cut(s) 132, 1669
AclI AACGTT 1 cut(s) 651
AclWI GGATC 6 cut(s) 84, 109, 122, 393, 409, 915
AcoI YGGCCR 2 cut(s) 69, 709
AcsI RAATTY 3 cut(s) 1385, 1586, 1981
AcuI CTGAAG 4 cut(s) 673, 1247, 1740, 1818
AcyI GRCGYC 1 cut(s) 500
AfaI GTAC 6 cut(s) 1093, 1565, 2085, 2258, 2378, 2618
AflII CTTAAG 1 cut(s) 269
AflIII ACRYGT 3 cut(s) 1375, 1964, 2403
AjiI CACGTC 1 cut(s) 1967
AjnI CCWGG 9 cut(s) 191, 1288, 1708, 1762, 2054, 2110, 2188, 2531, 2733
AjuI GAANNNNNNNTTGG 4 cut(s) 314, 346, 1980, 2012
AleI CACNNNNGTG 1 cut(s) 3103
AloI GAACNNNNNNTCC 2 cut(s) 2274, 2306
Alw21I GWGCWC 5 cut(s) 87, 2338, 2606, 2833, 2848
Alw26I GTCTC 3 cut(s) 468, 1645, 3036
AlwI GGATC 6 cut(s) 84, 109, 122, 393, 409, 915
AlwNI CAGNNNCTG 2 cut(s) 2744, 2798
Ama87I CYCGRG 3 cut(s) 490, 1461, 2331
Aor13HI TCCGGA 2 cut(s) 132, 1669
ApaI GGGCCC 1 cut(s) 2532
ApeKI GCWGC 6 cut(s) 609, 1609, 2120, 2361, 2795, 2864
ApoI RAATTY 3 cut(s) 1385, 1586, 1981
ArsI GACNNNNNNTTYG 4 cut(s) 298, 330, 977, 1009
Asp700I GAANNNNTTC 3 cut(s) 8, 401, 473
AspLEI GCGC 2 cut(s) 362, 1598
AsuC2I CCSGG 3 cut(s) 424, 491, 492
AsuHPI GGTGA 6 cut(s) 1076, 2202, 2253, 2425, 2771, 2960
AsuII TTCGAA 1 cut(s) 1799
AvaI CYCGRG 3 cut(s) 490, 1461, 2331
AvaII GGWCC 6 cut(s) 153, 195, 343, 494, 1281, 2741
BaeGI GKGCMC 3 cut(s) 1001, 2403, 2532
BamHI GGATCC 1 cut(s) 114
BanII GRGCYC 5 cut(s) 87, 2338, 2532, 2833, 3066
BbsI GAAGAC 1 cut(s) 2768
Bbv12I GWGCWC 5 cut(s) 87, 2338, 2606, 2833, 2848
BbvCI CCTCAGC 1 cut(s) 2319
BbvI GCAGC 6 cut(s) 596, 1621, 2132, 2373, 2807, 2876
BccI CCATC 4 cut(s) 944, 1624, 2760, 2914
BceAI ACGGC 4 cut(s) 218, 232, 262, 424
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 9 cut(s) 193, 1290, 1710, 1764, 2056, 2112, 2190, 2533, 2735
BciVI GTATCC 1 cut(s) 2831
BclI TGATCA 2 cut(s) 2032, 3007
BcnI CCSGG 3 cut(s) 424, 491, 492
BcoDI GTCTC 3 cut(s) 468, 1645, 3036
BfaI CTAG 5 cut(s) 1533, 1749, 2294, 2351, 2390
BfmI CTRYAG 4 cut(s) 878, 1248, 2340, 2862
BfrI CTTAAG 1 cut(s) 269
BfuAI ACCTGC 1 cut(s) 2729
BfuI GTATCC 1 cut(s) 2831
BglI GCCNNNNNGGC 2 cut(s) 68, 261
BglII AGATCT 1 cut(s) 1751
BisI GCNGC 9 cut(s) 220, 262, 610, 1610, 1924, 2121, 2362, 2796, 2865
BlpI GCTNAGC 1 cut(s) 177
BlsI GCNGC 9 cut(s) 221, 263, 611, 1611, 1925, 2122, 2363, 2797, 2866
Bme18I GGWCC 6 cut(s) 153, 195, 343, 494, 1281, 2741
BmeT110I CYCGRG 3 cut(s) 490, 1461, 2331
BmgBI CACGTC 1 cut(s) 1967
BmrI ACTGGG 2 cut(s) 795, 3001
BmsI GCATC 3 cut(s) 2287, 2500, 3042
BmuI ACTGGG 2 cut(s) 795, 3001
BoxI GACNNNNGTC 1 cut(s) 1260
BpiI GAAGAC 1 cut(s) 2768
BplI GAGNNNNNCTC 2 cut(s) 2593, 2625
BpmI CTGGAG 4 cut(s) 359, 1386, 2298, 2832
Bpu10I CCTNAGC 2 cut(s) 1605, 2319
Bpu1102I GCTNAGC 1 cut(s) 177
Bpu14I TTCGAA 1 cut(s) 1799
BpuEI CTTGAG 1 cut(s) 1279
BpuMI CCSGG 3 cut(s) 424, 491, 492
BsaBI GATNNNNATC 1 cut(s) 1820
BsaHI GRCGYC 1 cut(s) 500
BsaWI WCCGGW 3 cut(s) 132, 865, 1669
Bse118I RCCGGY 2 cut(s) 197, 711
Bse1I ACTGG 7 cut(s) 292, 712, 790, 2253, 2439, 2873, 2996
Bse3DI GCAATG 9 cut(s) 229, 299, 369, 619, 1001, 2303, 2802, 3045, 3081
Bse8I GATNNNNATC 1 cut(s) 1820
BseAI TCCGGA 2 cut(s) 132, 1669
BseBI CCWGG 9 cut(s) 193, 1290, 1710, 1764, 2056, 2112, 2190, 2533, 2735
BseGI GGATG 5 cut(s) 1616, 2257, 2491, 2752, 3085
BseJI GATNNNNATC 1 cut(s) 1820
BseMI GCAATG 9 cut(s) 229, 299, 369, 619, 1001, 2303, 2802, 3045, 3081
BseMII CTCAG 7 cut(s) 191, 325, 636, 1178, 1596, 2310, 3092
BseNI ACTGG 7 cut(s) 292, 712, 790, 2253, 2439, 2873, 2996
BseRI GAGGAG 3 cut(s) 884, 914, 1036
BseSI GKGCMC 3 cut(s) 1001, 2403, 2532
BseXI GCAGC 6 cut(s) 596, 1621, 2132, 2373, 2807, 2876
BseYI CCCAGC 1 cut(s) 637
Bsh1236I CGCG 1 cut(s) 395
BsiHKAI GWGCWC 5 cut(s) 87, 2338, 2606, 2833, 2848
BsiHKCI CYCGRG 3 cut(s) 490, 1461, 2331
BsiSI CCGG 8 cut(s) 133, 198, 424, 491, 497, 712, 866, 1670
BslFI GGGAC 7 cut(s) 329, 406, 569, 1478, 1951, 2445, 2861
BsmAI GTCTC 3 cut(s) 468, 1645, 3036
BsmFI GGGAC 7 cut(s) 329, 406, 569, 1478, 1951, 2445, 2861
BsoBI CYCGRG 3 cut(s) 490, 1461, 2331
Bsp119I TTCGAA 1 cut(s) 1799
Bsp120I GGGCCC 1 cut(s) 2528
Bsp1286I GDGCHC 9 cut(s) 87, 1001, 2338, 2403, 2532, 2606, 2833, 2848, 3066
Bsp13I TCCGGA 2 cut(s) 132, 1669
Bsp1407I TGTACA 1 cut(s) 2376
Bsp1720I GCTNAGC 1 cut(s) 177
Bsp19I CCATGG 2 cut(s) 2365, 2704
BspCNI CTCAG 7 cut(s) 190, 324, 637, 1179, 1597, 2311, 3093
BspEI TCCGGA 2 cut(s) 132, 1669
BspFNI CGCG 1 cut(s) 395
BspMAI CTGCAG 2 cut(s) 2344, 2866
BspMI ACCTGC 1 cut(s) 2729
BspPI GGATC 6 cut(s) 84, 109, 122, 393, 409, 915
BspQI GCTCTTC 2 cut(s) 1401, 3054
BspT104I TTCGAA 1 cut(s) 1799
BspTI CTTAAG 1 cut(s) 269
BsrBI CCGCTC 2 cut(s) 130, 353
BsrDI GCAATG 9 cut(s) 229, 299, 369, 619, 1001, 2303, 2802, 3045, 3081
BsrFI RCCGGY 2 cut(s) 197, 711
BsrGI TGTACA 1 cut(s) 2376
BsrI ACTGG 7 cut(s) 292, 712, 790, 2253, 2439, 2873, 2996
BssAI RCCGGY 2 cut(s) 197, 711
BssNI GRCGYC 1 cut(s) 500
BssT1I CCWWGG 4 cut(s) 1366, 2365, 2460, 2704
Bst2UI CCWGG 9 cut(s) 193, 1290, 1710, 1764, 2056, 2112, 2190, 2533, 2735
Bst6I CTCTTC 5 cut(s) 33, 479, 1309, 1401, 3054
BstACI GRCGYC 1 cut(s) 500
BstAFI CTTAAG 1 cut(s) 269
BstAUI TGTACA 1 cut(s) 2376
BstBI TTCGAA 1 cut(s) 1799
BstC8I GCNNGC 8 cut(s) 266, 837, 1234, 2073, 2606, 2699, 2800, 3090
BstDSI CCRYGG 2 cut(s) 2365, 2704
BstENI CCTNNNNNAGG 1 cut(s) 1365
BstF5I GGATG 5 cut(s) 1616, 2257, 2491, 2752, 3085
BstFNI CGCG 1 cut(s) 395
BstHHI GCGC 2 cut(s) 362, 1598
BstMAI GTCTC 3 cut(s) 468, 1645, 3036
BstNI CCWGG 9 cut(s) 193, 1290, 1710, 1764, 2056, 2112, 2190, 2533, 2735
BstNSI RCATGY 2 cut(s) 1379, 2407
BstPAI GACNNNNGTC 1 cut(s) 1260
BstSFI CTRYAG 4 cut(s) 878, 1248, 2340, 2862
BstSLI GKGCMC 3 cut(s) 1001, 2403, 2532
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 6 cut(s) 596, 1621, 2132, 2373, 2807, 2876
BstV2I GAAGAC 1 cut(s) 2768
BstX2I RGATCY 4 cut(s) 114, 385, 907, 1751
BstYI RGATCY 4 cut(s) 114, 385, 907, 1751
BsuI GTATCC 1 cut(s) 2831
BtgI CCRYGG 2 cut(s) 2365, 2704
BtrI CACGTC 1 cut(s) 1967
BtsCI GGATG 5 cut(s) 1616, 2257, 2491, 2752, 3085
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 8 cut(s) 285, 294, 783, 939, 1282, 1307, 2401, 3098
BveI ACCTGC 1 cut(s) 2729
Cac8I GCNNGC 8 cut(s) 266, 837, 1234, 2073, 2606, 2699, 2800, 3090
CaiI CAGNNNCTG 2 cut(s) 2744, 2798
CfoI GCGC 2 cut(s) 362, 1598
Cfr10I RCCGGY 2 cut(s) 197, 711
Cfr9I CCCGGG 1 cut(s) 490
CseI GACGC 2 cut(s) 489, 665
Csp6I GTAC 6 cut(s) 1092, 1564, 2084, 2257, 2377, 2617
CviAII CATG 9 cut(s) 150, 1376, 1477, 1693, 1904, 2366, 2404, 2687, 2705
CviQI GTAC 6 cut(s) 1092, 1564, 2084, 2257, 2377, 2617
DraI TTTAAA 2 cut(s) 1680, 2820
EaeI YGGCCR 2 cut(s) 69, 709
Eam1104I CTCTTC 5 cut(s) 33, 479, 1309, 1401, 3054
EarI CTCTTC 5 cut(s) 33, 479, 1309, 1401, 3054
EciI GGCGGA 2 cut(s) 49, 1386
Ecl136II GAGCTC 3 cut(s) 85, 2336, 2831
Eco130I CCWWGG 4 cut(s) 1366, 2365, 2460, 2704
Eco147I AGGCCT 3 cut(s) 1232, 2697, 3130
Eco24I GRGCYC 5 cut(s) 87, 2338, 2532, 2833, 3066
Eco47I GGWCC 6 cut(s) 153, 195, 343, 494, 1281, 2741
Eco53kI GAGCTC 3 cut(s) 85, 2336, 2831
Eco57I CTGAAG 4 cut(s) 673, 1247, 1740, 1818
Eco88I CYCGRG 3 cut(s) 490, 1461, 2331
EcoICRI GAGCTC 3 cut(s) 85, 2336, 2831
EcoNI CCTNNNNNAGG 1 cut(s) 1365
EcoO109I RGGNCCY 3 cut(s) 343, 2528, 2741
EcoRI GAATTC 1 cut(s) 1385
EcoRII CCWGG 9 cut(s) 191, 1288, 1708, 1762, 2054, 2110, 2188, 2531, 2733
EcoT14I CCWWGG 4 cut(s) 1366, 2365, 2460, 2704
EcoT38I GRGCYC 5 cut(s) 87, 2338, 2532, 2833, 3066
ErhI CCWWGG 4 cut(s) 1366, 2365, 2460, 2704
FaeI CATG 9 cut(s) 153, 1379, 1480, 1696, 1907, 2369, 2407, 2690, 2708
FalI AAGNNNNNCTT 4 cut(s) 1780, 1812, 2532, 2564
FaqI GGGAC 7 cut(s) 329, 406, 569, 1478, 1951, 2445, 2861
FatI CATG 9 cut(s) 149, 1375, 1476, 1692, 1903, 2365, 2403, 2686, 2704
FauI CCCGC 6 cut(s) 46, 135, 358, 476, 1198, 2418
FauNDI CATATG 1 cut(s) 2230
FbaI TGATCA 2 cut(s) 2032, 3007
Fnu4HI GCNGC 9 cut(s) 220, 262, 610, 1610, 1924, 2121, 2362, 2796, 2865
FokI GGATG 5 cut(s) 1603, 2264, 2478, 2739, 3092
FriOI GRGCYC 5 cut(s) 87, 2338, 2532, 2833, 3066
Fsp4HI GCNGC 9 cut(s) 220, 262, 610, 1610, 1924, 2121, 2362, 2796, 2865
FspBI CTAG 5 cut(s) 1533, 1749, 2294, 2351, 2390
GlaI GCGC 2 cut(s) 361, 1597
GluI GCNGC 9 cut(s) 220, 262, 610, 1610, 1924, 2121, 2362, 2796, 2865
GsaI CCCAGC 1 cut(s) 641
GsuI CTGGAG 4 cut(s) 359, 1386, 2298, 2832
HapII CCGG 8 cut(s) 133, 198, 424, 491, 497, 712, 866, 1670
HgaI GACGC 2 cut(s) 489, 665
HhaI GCGC 2 cut(s) 362, 1598
Hin1I GRCGYC 1 cut(s) 500
Hin1II CATG 9 cut(s) 153, 1379, 1480, 1696, 1907, 2369, 2407, 2690, 2708
Hin6I GCGC 2 cut(s) 360, 1596
HinP1I GCGC 2 cut(s) 360, 1596
HincII GTYRAC 4 cut(s) 1255, 2409, 2931, 2980
HindII GTYRAC 4 cut(s) 1255, 2409, 2931, 2980
HindIII AAGCTT 2 cut(s) 1138, 1684
HpaI GTTAAC 1 cut(s) 2931
HpaII CCGG 8 cut(s) 133, 198, 424, 491, 497, 712, 866, 1670
HphI GGTGA 6 cut(s) 1076, 2202, 2253, 2425, 2771, 2960
Hpy166II GTNNAC 7 cut(s) 205, 1255, 1547, 2257, 2409, 2931, 2980
Hpy8I GTNNAC 7 cut(s) 205, 1255, 1547, 2257, 2409, 2931, 2980
Hpy99I CGWCG 4 cut(s) 218, 233, 248, 505
HpyAV CCTTC 9 cut(s) 807, 1222, 1227, 1466, 1795, 1980, 2169, 2889, 3008
HpyCH4IV ACGT 3 cut(s) 651, 1966, 2895
HpySE526I ACGT 3 cut(s) 651, 1966, 2895
Hsp92I GRCGYC 1 cut(s) 500
Hsp92II CATG 9 cut(s) 153, 1379, 1480, 1696, 1907, 2369, 2407, 2690, 2708
HspAI GCGC 2 cut(s) 360, 1596
KflI GGGWCCC 1 cut(s) 343
Kpn2I TCCGGA 2 cut(s) 132, 1669
Ksp22I TGATCA 2 cut(s) 2032, 3007
KspAI GTTAAC 1 cut(s) 2931
LguI GCTCTTC 2 cut(s) 1401, 3054
LmnI GCTCC 8 cut(s) 82, 90, 135, 790, 2341, 2809, 2828, 2851
Lsp1109I GCAGC 6 cut(s) 596, 1621, 2132, 2373, 2807, 2876
LweI GCATC 3 cut(s) 2287, 2500, 3042
MaeI CTAG 5 cut(s) 1533, 1749, 2294, 2351, 2390
MaeII ACGT 3 cut(s) 651, 1966, 2895
MaeIII GTNAC 6 cut(s) 235, 677, 1472, 1580, 1843, 2948
MbiI CCGCTC 2 cut(s) 130, 353
MfeI CAATTG 1 cut(s) 1413
MflI RGATCY 4 cut(s) 114, 385, 907, 1751
MhlI GDGCHC 9 cut(s) 87, 1001, 2338, 2403, 2532, 2606, 2833, 2848, 3066
MlyI GAGTC 3 cut(s) 86, 1236, 1270
MmeI TCCRAC 5 cut(s) 60, 690, 885, 1059, 1819
MroI TCCGGA 2 cut(s) 132, 1669
MroXI GAANNNNTTC 3 cut(s) 8, 401, 473
MslI CAYNNNNRTG 4 cut(s) 1347, 1380, 2595, 3103
MspA1I CMGCKG 2 cut(s) 2318, 2798
MspCI CTTAAG 1 cut(s) 269
MspI CCGG 8 cut(s) 133, 198, 424, 491, 497, 712, 866, 1670
MunI CAATTG 1 cut(s) 1413
MvaI CCWGG 9 cut(s) 193, 1290, 1710, 1764, 2056, 2112, 2190, 2533, 2735
MvnI CGCG 1 cut(s) 395
NciI CCSGG 3 cut(s) 424, 491, 492
NcoI CCATGG 2 cut(s) 2365, 2704
NdeI CATATG 1 cut(s) 2230
NlaIII CATG 9 cut(s) 153, 1379, 1480, 1696, 1907, 2369, 2407, 2690, 2708
NmeAIII GCCGAG 4 cut(s) 47, 231, 237, 2737
NmuCI GTSAC 2 cut(s) 677, 2948
NspI RCATGY 2 cut(s) 1379, 2407
NspV TTCGAA 1 cut(s) 1799
OliI CACNNNNGTG 1 cut(s) 3103
PaeR7I CTCGAG 1 cut(s) 2331
PasI CCCWGGG 1 cut(s) 2532
PceI AGGCCT 3 cut(s) 1232, 2697, 3130
PciI ACATGT 2 cut(s) 1375, 2403
PciSI GCTCTTC 2 cut(s) 1401, 3054
PdmI GAANNNNTTC 3 cut(s) 8, 401, 473
PfeI GAWTC 8 cut(s) 469, 505, 728, 775, 973, 1327, 1959, 2143
PflMI CCANNNNNTGG 2 cut(s) 382, 2093
PfoI TCCNGGA 2 cut(s) 422, 1708
PkrI GCNGC 9 cut(s) 221, 263, 611, 1611, 1925, 2122, 2363, 2797, 2866
PleI GAGTC 3 cut(s) 85, 1236, 1269
PpsI GAGTC 3 cut(s) 85, 1236, 1269
PpuMI RGGWCCY 2 cut(s) 343, 2741
PscI ACATGT 2 cut(s) 1375, 2403
PshAI GACNNNNGTC 1 cut(s) 1260
Psp124BI GAGCTC 3 cut(s) 87, 2338, 2833
Psp1406I AACGTT 1 cut(s) 651
Psp5II RGGWCCY 2 cut(s) 343, 2741
Psp6I CCWGG 9 cut(s) 191, 1288, 1708, 1762, 2054, 2110, 2188, 2531, 2733
PspFI CCCAGC 1 cut(s) 637
PspGI CCWGG 9 cut(s) 191, 1288, 1708, 1762, 2054, 2110, 2188, 2531, 2733
PspOMI GGGCCC 1 cut(s) 2528
PspPPI RGGWCCY 2 cut(s) 343, 2741
PsrI GAACNNNNNNTAC 2 cut(s) 389, 421
PstI CTGCAG 2 cut(s) 2344, 2866
PstNI CAGNNNCTG 2 cut(s) 2744, 2798
PsuI RGATCY 4 cut(s) 114, 385, 907, 1751
PvuII CAGCTG 2 cut(s) 2318, 2798
RsaI GTAC 6 cut(s) 1093, 1565, 2085, 2258, 2378, 2618
RsaNI GTAC 6 cut(s) 1092, 1564, 2084, 2257, 2377, 2617
RseI CAYNNNNRTG 4 cut(s) 1347, 1380, 2595, 3103
SacI GAGCTC 3 cut(s) 87, 2338, 2833
SapI GCTCTTC 2 cut(s) 1401, 3054
SatI GCNGC 9 cut(s) 220, 262, 610, 1610, 1924, 2121, 2362, 2796, 2865
SchI GAGTC 3 cut(s) 86, 1236, 1270
SduI GDGCHC 9 cut(s) 87, 1001, 2338, 2403, 2532, 2606, 2833, 2848, 3066
SfaNI GCATC 3 cut(s) 2287, 2500, 3042
SfcI CTRYAG 4 cut(s) 878, 1248, 2340, 2862
Sfr274I CTCGAG 1 cut(s) 2331
SfuI TTCGAA 1 cut(s) 1799
SinI GGWCC 6 cut(s) 153, 195, 343, 494, 1281, 2741
SlaI CTCGAG 1 cut(s) 2331
SmaI CCCGGG 1 cut(s) 492
SmiMI CAYNNNNRTG 4 cut(s) 1347, 1380, 2595, 3103
SmlI CTYRAG 3 cut(s) 269, 1258, 2331
SmoI CTYRAG 3 cut(s) 269, 1258, 2331
SseBI AGGCCT 3 cut(s) 1232, 2697, 3130
SspMI CTAG 5 cut(s) 1533, 1749, 2294, 2351, 2390
SstI GAGCTC 3 cut(s) 87, 2338, 2833
StuI AGGCCT 3 cut(s) 1232, 2697, 3130
StyI CCWWGG 4 cut(s) 1366, 2365, 2460, 2704
TaiI ACGT 3 cut(s) 654, 1969, 2898
TaqII GACCGA 1 cut(s) 170
TatI WGTACW 2 cut(s) 2256, 2376
TauI GCSGC 3 cut(s) 222, 264, 1926
TfiI GAWTC 8 cut(s) 469, 505, 728, 775, 973, 1327, 1959, 2143
TscAI CASTG 8 cut(s) 292, 301, 790, 946, 1282, 1314, 2401, 3105
TseFI GTSAC 2 cut(s) 677, 2948
TseI GCWGC 6 cut(s) 609, 1609, 2120, 2361, 2795, 2864
Tsp45I GTSAC 2 cut(s) 677, 2948
TspMI CCCGGG 1 cut(s) 490
TspRI CASTG 8 cut(s) 292, 301, 790, 946, 1282, 1314, 2401, 3105
Van91I CCANNNNNTGG 2 cut(s) 382, 2093
Vha464I CTTAAG 1 cut(s) 269
VpaK11BI GGWCC 6 cut(s) 153, 195, 343, 494, 1281, 2741
XagI CCTNNNNNAGG 1 cut(s) 1365
XapI RAATTY 3 cut(s) 1385, 1586, 1981
XbaI TCTAGA 1 cut(s) 2389
XceI RCATGY 2 cut(s) 1379, 2407
XhoI CTCGAG 1 cut(s) 2331
XmaI CCCGGG 1 cut(s) 490
XmnI GAANNNNTTC 3 cut(s) 8, 401, 473
XspI CTAG 5 cut(s) 1533, 1749, 2294, 2351, 2390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.