Rroxscaffold_2G00127470
NAC Family

inactive receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016140
Physical Location & Seq
Forward (+)
62926905 .. 62932950
6046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00127470.1

Sequence Viewer

Length: 3198 bp
ATGATTTTTAGGGTTGTTTCGGCTGCAATGAACAGTTTCACTCTCACTCTTCTCTACTTGTTCTTTGTTTTGGCGGGTTCCGCCTACTCGGCCACCGAGTCGGAGCTCCGATCCCTCTACGAGTTCAAGAAAGGGATCCAAATTGACCCTCTCCGGAAAGTCCTCGACACCTGGACCGACATCTCACTCAACCAAGCTCAGCCTTGCCCTCCCTGGACCGGCGTTTACTGCAACGACGGCGGCAATGTCGTCGCCGTGGCACTCGACGGCCTCGGCCTCGGCGGCGAGCTTAAGTTGAATACCCTCACTGGCCTCACTGCATTGCAAAACCTCAGTCTTGCCAACAATGACTTCACGGGTCGGGTCCCACCGGCTCTGGGCGCAATGTCCTCCCTCCGGCACTTGGATCTGTCCGCGAACCGGTTCTACGGGCCGATCCCGTCCCGGATTAACGACTTGTGGGGCTTGAATTATCTCAACTTGTCCCGGAATCTCTTCAAAGGTGGGTTCCCGGGTCCGGGGTCGAATCTCAATCAGCTCAAAGTGTTCGATGTGCATTACAATCAGCTGTGGGGGGACATTTCGGATTTGTTGCAGGAGTTTCATAATGTGGAGTATGTTGATTTGAGCTACAATGAGTTTTTCGGAGGGATTGAGCTGGGTTCTGAGAACATTTCGAGCTTATCCAACACCGTGCGTCACTTGAACTTCAGCCACAATAATCTGACTGGCCGCTTCTTTAAGGCTGATTCAATTGAGTTGTTTAGGAACTTGCAAGTTTTGGACTTGGGTGGGAATCAGATCACTGGGGAGCTTCCTTCATTTGGGTCGTTGCCGAATTTACGAGTTTTGAGGCTGGCGAATAATCAGTTCTTTGGGGAAATACCGGAGGAGTTGCTACAGAGTTCTATTCCAATGGAGGAGTTGGATCTTAGTGGAAATGCTTTTACAGGTTCCATCACTGGAATTAAATCTACAACTTTGAAAGTCTTGAATCTGTCATCAAATGGTATGTCGGGCACATTGCAGAATGTGGATATGAGGAGTTGTGTTGTGGTAGATTTGAGCAGAAATAAGATTTCAGGTGATATATCAATGTTGGAGAATATGGGTACTGCTTTAGAGGTTCTCGATTTGAGTTCAAACAATTTTTCTGGAAGCTTAACAAACTTGACTATGCAGTTCGAGAGATTAACTACACTTAGTCTGAGAGATAATTTATTAGCAGGGTCTTTGCCTTCGATACTGAAGGCCTGCCCCAGACTCTCTACAGTTGACTTGAGTCAAAATGATTTCAGTGGGTCCATTCCTGGTAGTTTCTTCTCTTCACTGACATTGACAAGGTTGAATCTTTCACGAAATCACTTAAATGGGCCAATTCCTCTCCAAGGCGGACATGTAAGCGAATTCTTAGCTCTTCCTCCAGATTTGCCAATTGAGAATGTCGATCTCTCCAATAATTCTTTATCGGGTTCCCTTCCTCGGGACATTGGTAACATGGTGGAACTCAAATTGCTAAACCTTGCAAAGAATGAGTTTTCAGGAGAACTACCAAGTGAATTGAGTAAACTTGCTAAGTTGAAGTACCTTGATTTATCAGGTAACAAATTCAAGGGCGCAATTCCTGAGCAGCTTCCATCCAGCTTAATTGTATTTAATGTCTCCTATAACGATCTATCTGGTTCTGTTCCGGACAATTTAAAAAGCTTCCCCATGACTTCATTTCGTCCTGGAAATGAGTTGCTAAACTTACCAAATGATGATCGGGCTAGATCTTCAGTTCCAGGTCGTATTCCAGAACAAGGAAGAAGTCATACTTCGAAGGCTCATATTAGGATAGCAATCATTGTTGCTTCAGTTGGAGTTACTTTGATGATAGTTTTTGTTTTGTTGGTTTATCATCAGACACACCATAAAGGATTTCATGGAAGAAATGGATTTGGCGGAGAAAATACAGGGACAGATGTTAAGATTGGCAGATTCACACGTCCTTCATTTTTGAATTTCCAAACGAATGTTCAGCCTCCACCAACTTCATTGAGCTTTTCAAATGATCACTTGCTAACTTCACAATCCAGGTCATTGTCAGGGCAGGCAGAGTTTGTACCAGAAATTGGGAAGCCTGTTTTACCTGGGGAAGCAGCAACGAGTTCTACACCTATGAATCTTCTTGATAACCAGCCTACAACTTCTGGAAGGAAGTCCTCCCCTGGTTCTCCACTATCTTCCTCACCTCGTTTTATAGAGGCATGTGAGCAACCTGTAATACTGGATGTGTACTCACCAGATCGGTTGGCTGGAGAACTGTTCTTCCTAGATGCTTCATTGCAATTTACAGCTGAGGAATTGTCTCGAGCTCCTGCAGAAGTTCTGGGCAGAAGCAGCCATGGAACTCTGTACAAAGCTACTCTAGATAGTGGGCACATGTTGACGGTGAAATGGTTACGGGTGGGACTGGTCAAGCATAAGAAAGATTTTGCCAAGGAAGTCAAAAGGATTGGCTCTATAAGGCATCCAAACATTGTTCCATTACAGGCATACTATTGGGGGCCTAGGGAACAAGAGAGGCTTCTTTTAGCGGACTATGTTCAGGGAGATAGCTTGGCCCTACATCTTTATGAGAGCACGCCTCGGAGGTACTCCCCGTTATCATTCAACCAGAGGCTAAAAGTAGCTGTGGAGGTTGCTCGATGTTTGCTATACCTACATGATAGAGGCCTGCCCCATGGGAACCTAAAGCCAACTAATGTAATCCTGGCAGGTCCTGAGTACCATCCTCGGCTTACTGATTACAGTCTTCACCGTCTAATGACACCAGCTGGCATTGCGGAGCAGTTTTTAAATATGGGAGCTCTTGGATACCGTGCTCCAGAACTTGCTACTGCAGCAAAACCAGTCCCTTCTTTCAAAGCTGACGTTTATGCCTTTGGAGTGATTTTGATGGAAATGTTAACCAGAAGAAGTGCTGGTGACATTATATCGGGTCAGTCAGGGGCGGTTGACCTTACAGACTGGGTTAGGCTTTGTGATCAAGAAGGACGAGGAATGGATTGCATCGACAGAGACATTGCTGGTGGAGAAGAACCCTCAAAAGCAATGGATGAATTGCTTGCAATATCACTGAGGTGTATTCTCCCTGTAAATGAGAGGCCTAACATCAGACAAGTATTTGACAACCTCTGTTCTATATCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase

Protein Analysis

1065

Amino Acids

116.66

Weight (kDa)

6.12

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_4 PF12799 257 - 291 1.6e-08 Leucine Rich repeats (2 copies)
LRR_8 PF13855 480 - 537 5.2e-06 Leucine rich repeat
Pkinase PF00069 789 - 991 1.6e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 789 - 986 2.5e-21 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016140)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10020 AT5G10020
fragaria_vesca FvH4_1g22060
malus_domestica MD01G1018200.v1.1
pyrus_communis pycom01g04790
rosa_chinensis RchiOBHm_Chr2g0116131
rosa_laevigata RLG00000018242
rosa_multiflora Rmu_sc0002288.1_g000016
rosa_roxburghii Rroxscaffold_2G00127470
rosa_rugosa Rorug02G0203700
rosa_samantha Rh2AG260100 Rh2BG271100 Rh2CG266400 Rh2DG268300
rosa_wichuraiana Rw2G020370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2750
AccB7I CCANNNNNTGG 1 cut(s) 2114
AccII CGCG 1 cut(s) 416
AccIII TCCGGA 2 cut(s) 153, 1690
AclWI GGATC 6 cut(s) 105, 130, 143, 414, 430, 936
AcoI YGGCCR 2 cut(s) 90, 730
AcsI RAATTY 4 cut(s) 838, 1406, 1607, 2002
AcuI CTGAAG 4 cut(s) 694, 1268, 1761, 1839
AfaI GTAC 7 cut(s) 1114, 1586, 2106, 2279, 2399, 2639, 2771
AflII CTTAAG 1 cut(s) 290
AflIII ACRYGT 3 cut(s) 1396, 1985, 2424
AgeI ACCGGT 1 cut(s) 420
AjiI CACGTC 1 cut(s) 1988
AjnI CCWGG 9 cut(s) 170, 212, 1309, 1729, 1783, 2075, 2131, 2209, 2754
AjuI GAANNNNNNNTTGG 4 cut(s) 335, 367, 2001, 2033
AleI CACNNNNGTG 1 cut(s) 3124
AloI GAACNNNNNNTCC 2 cut(s) 2295, 2327
Alw21I GWGCWC 5 cut(s) 108, 2359, 2627, 2854, 2869
Alw26I GTCTC 3 cut(s) 1666, 2355, 3057
AlwI GGATC 6 cut(s) 105, 130, 143, 414, 430, 936
AlwNI CAGNNNCTG 1 cut(s) 2765
Ama87I CYCGRG 3 cut(s) 511, 1482, 2352
Aor13HI TCCGGA 2 cut(s) 153, 1690
ApeKI GCWGC 5 cut(s) 23, 1630, 2141, 2382, 2885
ApoI RAATTY 4 cut(s) 838, 1406, 1607, 2002
ArsI GACNNNNNNTTYG 4 cut(s) 319, 351, 998, 1030
AsiGI ACCGGT 1 cut(s) 420
Asp700I GAANNNNTTC 3 cut(s) 35, 422, 494
AspA2I CCTAGG 1 cut(s) 2552
AspLEI GCGC 2 cut(s) 383, 1619
AsuC2I CCSGG 5 cut(s) 445, 487, 512, 513, 519
AsuHPI GGTGA 6 cut(s) 1097, 2223, 2274, 2446, 2792, 2981
AsuII TTCGAA 1 cut(s) 1820
AvaI CYCGRG 3 cut(s) 511, 1482, 2352
AvaII GGWCC 6 cut(s) 174, 216, 364, 515, 1302, 2762
AvrII CCTAGG 1 cut(s) 2552
BaeGI GKGCMC 2 cut(s) 1022, 2424
BamHI GGATCC 1 cut(s) 135
BanII GRGCYC 3 cut(s) 108, 2359, 2854
BarI GAAGNNNNNNTAC 2 cut(s) 1798, 1830
BbsI GAAGAC 1 cut(s) 2789
Bbv12I GWGCWC 5 cut(s) 108, 2359, 2627, 2854, 2869
BbvCI CCTCAGC 1 cut(s) 2340
BbvI GCAGC 5 cut(s) 10, 1642, 2153, 2394, 2897
BccI CCATC 4 cut(s) 965, 1645, 2781, 2935
BceAI ACGGC 3 cut(s) 239, 253, 283
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 9 cut(s) 172, 214, 1311, 1731, 1785, 2077, 2133, 2211, 2756
BciVI GTATCC 1 cut(s) 2852
BclI TGATCA 2 cut(s) 2053, 3028
BcnI CCSGG 5 cut(s) 445, 487, 512, 513, 519
BcoDI GTCTC 3 cut(s) 1666, 2355, 3057
BfaI CTAG 4 cut(s) 1770, 2315, 2411, 2553
BfmI CTRYAG 4 cut(s) 899, 1269, 2361, 2883
BfrI CTTAAG 1 cut(s) 290
BfuAI ACCTGC 1 cut(s) 2750
BfuI GTATCC 1 cut(s) 2852
BglI GCCNNNNNGGC 2 cut(s) 89, 282
BglII AGATCT 1 cut(s) 1772
BisI GCNGC 8 cut(s) 24, 241, 283, 733, 1631, 2142, 2383, 2886
BlnI CCTAGG 1 cut(s) 2552
BlpI GCTNAGC 1 cut(s) 198
BlsI GCNGC 8 cut(s) 25, 242, 284, 734, 1632, 2143, 2384, 2887
Bme18I GGWCC 6 cut(s) 174, 216, 364, 515, 1302, 2762
BmeT110I CYCGRG 3 cut(s) 511, 1482, 2352
BmgBI CACGTC 1 cut(s) 1988
BmrI ACTGGG 2 cut(s) 816, 3022
BmsI GCATC 3 cut(s) 2308, 2521, 3063
BmuI ACTGGG 2 cut(s) 816, 3022
BoxI GACNNNNGTC 1 cut(s) 1281
BpiI GAAGAC 1 cut(s) 2789
BplI GAGNNNNNCTC 2 cut(s) 2614, 2646
BpmI CTGGAG 3 cut(s) 1407, 2319, 2853
Bpu10I CCTNAGC 2 cut(s) 1626, 2340
Bpu1102I GCTNAGC 1 cut(s) 198
Bpu14I TTCGAA 1 cut(s) 1820
BpuEI CTTGAG 1 cut(s) 1300
BpuMI CCSGG 5 cut(s) 445, 487, 512, 513, 519
BsaBI GATNNNNATC 1 cut(s) 1841
BsaWI WCCGGW 4 cut(s) 153, 420, 886, 1690
Bse118I RCCGGY 3 cut(s) 218, 370, 420
Bse1I ACTGG 8 cut(s) 313, 733, 811, 967, 2274, 2460, 2894, 3017
Bse3DI GCAATG 9 cut(s) 33, 250, 320, 390, 1022, 2324, 2823, 3066, 3102
Bse8I GATNNNNATC 1 cut(s) 1841
BseAI TCCGGA 2 cut(s) 153, 1690
BseBI CCWGG 9 cut(s) 172, 214, 1311, 1731, 1785, 2077, 2133, 2211, 2756
BseGI GGATG 5 cut(s) 1637, 2278, 2512, 2773, 3106
BseJI GATNNNNATC 1 cut(s) 1841
BseMI GCAATG 9 cut(s) 33, 250, 320, 390, 1022, 2324, 2823, 3066, 3102
BseMII CTCAG 8 cut(s) 212, 346, 657, 1199, 1617, 2331, 2757, 3113
BseNI ACTGG 8 cut(s) 313, 733, 811, 967, 2274, 2460, 2894, 3017
BseRI GAGGAG 3 cut(s) 905, 935, 1057
BseSI GKGCMC 2 cut(s) 1022, 2424
BseXI GCAGC 5 cut(s) 10, 1642, 2153, 2394, 2897
BseYI CCCAGC 1 cut(s) 658
Bsh1236I CGCG 1 cut(s) 416
BshTI ACCGGT 1 cut(s) 420
BsiHKAI GWGCWC 5 cut(s) 108, 2359, 2627, 2854, 2869
BsiHKCI CYCGRG 3 cut(s) 511, 1482, 2352
BslFI GGGAC 8 cut(s) 350, 427, 469, 590, 1499, 1972, 2466, 2882
BsmAI GTCTC 3 cut(s) 1666, 2355, 3057
BsmFI GGGAC 8 cut(s) 350, 427, 469, 590, 1499, 1972, 2466, 2882
BsoBI CYCGRG 3 cut(s) 511, 1482, 2352
Bsp119I TTCGAA 1 cut(s) 1820
Bsp1286I GDGCHC 7 cut(s) 108, 1022, 2359, 2424, 2627, 2854, 2869
Bsp13I TCCGGA 2 cut(s) 153, 1690
Bsp1407I TGTACA 1 cut(s) 2397
Bsp1720I GCTNAGC 1 cut(s) 198
Bsp19I CCATGG 2 cut(s) 2386, 2725
BspCNI CTCAG 8 cut(s) 211, 345, 658, 1200, 1618, 2332, 2758, 3114
BspEI TCCGGA 2 cut(s) 153, 1690
BspFNI CGCG 1 cut(s) 416
BspMAI CTGCAG 2 cut(s) 2365, 2887
BspMI ACCTGC 1 cut(s) 2750
BspPI GGATC 6 cut(s) 105, 130, 143, 414, 430, 936
BspQI GCTCTTC 1 cut(s) 1422
BspT104I TTCGAA 1 cut(s) 1820
BspTI CTTAAG 1 cut(s) 290
BsrDI GCAATG 9 cut(s) 33, 250, 320, 390, 1022, 2324, 2823, 3066, 3102
BsrFI RCCGGY 3 cut(s) 218, 370, 420
BsrGI TGTACA 1 cut(s) 2397
BsrI ACTGG 8 cut(s) 313, 733, 811, 967, 2274, 2460, 2894, 3017
BssAI RCCGGY 3 cut(s) 218, 370, 420
BssT1I CCWWGG 5 cut(s) 1387, 2386, 2481, 2552, 2725
Bst2UI CCWGG 9 cut(s) 172, 214, 1311, 1731, 1785, 2077, 2133, 2211, 2756
Bst4CI ACNGT 8 cut(s) 35, 694, 1273, 2307, 2434, 2795, 2804, 2864
Bst6I CTCTTC 4 cut(s) 54, 500, 1330, 1422
BstAFI CTTAAG 1 cut(s) 290
BstAUI TGTACA 1 cut(s) 2397
BstBI TTCGAA 1 cut(s) 1820
BstC8I GCNNGC 8 cut(s) 287, 858, 1255, 2094, 2627, 2720, 2821, 3111
BstDSI CCRYGG 3 cut(s) 255, 2386, 2725
BstENI CCTNNNNNAGG 1 cut(s) 1386
BstF5I GGATG 5 cut(s) 1637, 2278, 2512, 2773, 3106
BstFNI CGCG 1 cut(s) 416
BstHHI GCGC 2 cut(s) 383, 1619
BstMAI GTCTC 3 cut(s) 1666, 2355, 3057
BstMWI GCNNNNNNNGC 9 cut(s) 80, 89, 228, 237, 282, 380, 2382, 2825, 2885
BstNI CCWGG 9 cut(s) 172, 214, 1311, 1731, 1785, 2077, 2133, 2211, 2756
BstNSI RCATGY 3 cut(s) 1400, 2253, 2428
BstPAI GACNNNNGTC 1 cut(s) 1281
BstSFI CTRYAG 4 cut(s) 899, 1269, 2361, 2883
BstSLI GKGCMC 2 cut(s) 1022, 2424
BstUI CGCG 1 cut(s) 416
BstV1I GCAGC 5 cut(s) 10, 1642, 2153, 2394, 2897
BstV2I GAAGAC 1 cut(s) 2789
BstX2I RGATCY 4 cut(s) 135, 406, 928, 1772
BstYI RGATCY 4 cut(s) 135, 406, 928, 1772
BsuI GTATCC 1 cut(s) 2852
BtgI CCRYGG 3 cut(s) 255, 2386, 2725
BtrI CACGTC 1 cut(s) 1988
BtsCI GGATG 5 cut(s) 1637, 2278, 2512, 2773, 3106
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 7 cut(s) 306, 315, 804, 960, 1303, 1328, 3119
BveI ACCTGC 1 cut(s) 2750
Cac8I GCNNGC 8 cut(s) 287, 858, 1255, 2094, 2627, 2720, 2821, 3111
CaiI CAGNNNCTG 1 cut(s) 2765
CfoI GCGC 2 cut(s) 383, 1619
Cfr10I RCCGGY 3 cut(s) 218, 370, 420
Cfr9I CCCGGG 1 cut(s) 511
CseI GACGC 1 cut(s) 686
Csp6I GTAC 7 cut(s) 1113, 1585, 2105, 2278, 2398, 2638, 2770
CspAI ACCGGT 1 cut(s) 420
CviAII CATG 9 cut(s) 1397, 1498, 1714, 1925, 2250, 2387, 2425, 2708, 2726
CviQI GTAC 7 cut(s) 1113, 1585, 2105, 2278, 2398, 2638, 2770
DraI TTTAAA 2 cut(s) 1701, 2841
EaeI YGGCCR 2 cut(s) 90, 730
Eam1104I CTCTTC 4 cut(s) 54, 500, 1330, 1422
EarI CTCTTC 4 cut(s) 54, 500, 1330, 1422
EciI GGCGGA 3 cut(s) 70, 1407, 1959
Ecl136II GAGCTC 3 cut(s) 106, 2357, 2852
Eco130I CCWWGG 5 cut(s) 1387, 2386, 2481, 2552, 2725
Eco147I AGGCCT 3 cut(s) 1253, 2718, 3151
Eco24I GRGCYC 3 cut(s) 108, 2359, 2854
Eco47I GGWCC 6 cut(s) 174, 216, 364, 515, 1302, 2762
Eco53kI GAGCTC 3 cut(s) 106, 2357, 2852
Eco57I CTGAAG 4 cut(s) 694, 1268, 1761, 1839
Eco88I CYCGRG 3 cut(s) 511, 1482, 2352
EcoICRI GAGCTC 3 cut(s) 106, 2357, 2852
EcoNI CCTNNNNNAGG 1 cut(s) 1386
EcoO109I RGGNCCY 3 cut(s) 364, 2549, 2762
EcoRI GAATTC 1 cut(s) 1406
EcoRII CCWGG 9 cut(s) 170, 212, 1309, 1729, 1783, 2075, 2131, 2209, 2754
EcoT14I CCWWGG 5 cut(s) 1387, 2386, 2481, 2552, 2725
EcoT38I GRGCYC 3 cut(s) 108, 2359, 2854
ErhI CCWWGG 5 cut(s) 1387, 2386, 2481, 2552, 2725
FaeI CATG 9 cut(s) 1400, 1501, 1717, 1928, 2253, 2390, 2428, 2711, 2729
FalI AAGNNNNNCTT 4 cut(s) 1801, 1833, 2553, 2585
FaqI GGGAC 8 cut(s) 350, 427, 469, 590, 1499, 1972, 2466, 2882
FatI CATG 9 cut(s) 1396, 1497, 1713, 1924, 2249, 2386, 2424, 2707, 2725
FauI CCCGC 1 cut(s) 67
FbaI TGATCA 2 cut(s) 2053, 3028
Fnu4HI GCNGC 8 cut(s) 24, 241, 283, 733, 1631, 2142, 2383, 2886
FokI GGATG 5 cut(s) 1624, 2285, 2499, 2760, 3113
FriOI GRGCYC 3 cut(s) 108, 2359, 2854
Fsp4HI GCNGC 8 cut(s) 24, 241, 283, 733, 1631, 2142, 2383, 2886
FspBI CTAG 4 cut(s) 1770, 2315, 2411, 2553
GlaI GCGC 2 cut(s) 382, 1618
GluI GCNGC 8 cut(s) 24, 241, 283, 733, 1631, 2142, 2383, 2886
GsaI CCCAGC 1 cut(s) 662
GsuI CTGGAG 3 cut(s) 1407, 2319, 2853
HgaI GACGC 1 cut(s) 686
HhaI GCGC 2 cut(s) 383, 1619
Hin1II CATG 9 cut(s) 1400, 1501, 1717, 1928, 2253, 2390, 2428, 2711, 2729
Hin6I GCGC 2 cut(s) 381, 1617
HinP1I GCGC 2 cut(s) 381, 1617
HincII GTYRAC 4 cut(s) 1276, 2430, 2952, 3001
HindII GTYRAC 4 cut(s) 1276, 2430, 2952, 3001
HindIII AAGCTT 2 cut(s) 1159, 1705
HpaI GTTAAC 1 cut(s) 2952
HphI GGTGA 6 cut(s) 1097, 2223, 2274, 2446, 2792, 2981
Hpy166II GTNNAC 7 cut(s) 226, 1276, 1568, 2278, 2430, 2952, 3001
Hpy8I GTNNAC 7 cut(s) 226, 1276, 1568, 2278, 2430, 2952, 3001
Hpy99I CGWCG 3 cut(s) 239, 254, 269
HpyAV CCTTC 9 cut(s) 828, 1243, 1248, 1487, 1816, 2001, 2190, 2910, 3029
HpyCH4III ACNGT 8 cut(s) 35, 694, 1273, 2307, 2434, 2795, 2804, 2864
HpyCH4IV ACGT 2 cut(s) 1987, 2916
HpyF10VI GCNNNNNNNGC 9 cut(s) 80, 89, 228, 237, 282, 380, 2382, 2825, 2885
HpySE526I ACGT 2 cut(s) 1987, 2916
Hsp92II CATG 9 cut(s) 1400, 1501, 1717, 1928, 2253, 2390, 2428, 2711, 2729
HspAI GCGC 2 cut(s) 381, 1617
KflI GGGWCCC 1 cut(s) 364
Kpn2I TCCGGA 2 cut(s) 153, 1690
Ksp22I TGATCA 2 cut(s) 2053, 3028
KspAI GTTAAC 1 cut(s) 2952
LguI GCTCTTC 1 cut(s) 1422
LmnI GCTCC 7 cut(s) 103, 111, 811, 2362, 2830, 2849, 2872
Lsp1109I GCAGC 5 cut(s) 10, 1642, 2153, 2394, 2897
LweI GCATC 3 cut(s) 2308, 2521, 3063
MaeI CTAG 4 cut(s) 1770, 2315, 2411, 2553
MaeII ACGT 2 cut(s) 1987, 2916
MaeIII GTNAC 6 cut(s) 698, 1493, 1601, 1864, 2442, 2969
MfeI CAATTG 2 cut(s) 753, 1434
MflI RGATCY 4 cut(s) 135, 406, 928, 1772
MhlI GDGCHC 7 cut(s) 108, 1022, 2359, 2424, 2627, 2854, 2869
MlyI GAGTC 3 cut(s) 107, 1257, 1291
MmeI TCCRAC 5 cut(s) 81, 711, 906, 1080, 1840
MroI TCCGGA 2 cut(s) 153, 1690
MroXI GAANNNNTTC 3 cut(s) 35, 422, 494
MslI CAYNNNNRTG 3 cut(s) 1368, 2616, 3124
MspA1I CMGCKG 3 cut(s) 568, 2339, 2819
MspCI CTTAAG 1 cut(s) 290
MunI CAATTG 2 cut(s) 753, 1434
MvaI CCWGG 9 cut(s) 172, 214, 1311, 1731, 1785, 2077, 2133, 2211, 2756
MvnI CGCG 1 cut(s) 416
MwoI GCNNNNNNNGC 9 cut(s) 80, 89, 228, 237, 282, 380, 2382, 2825, 2885
NciI CCSGG 5 cut(s) 445, 487, 512, 513, 519
NcoI CCATGG 2 cut(s) 2386, 2725
NlaIII CATG 9 cut(s) 1400, 1501, 1717, 1928, 2253, 2390, 2428, 2711, 2729
NmeAIII GCCGAG 4 cut(s) 68, 252, 258, 2758
NmuCI GTSAC 2 cut(s) 698, 2969
NspI RCATGY 3 cut(s) 1400, 2253, 2428
NspV TTCGAA 1 cut(s) 1820
OliI CACNNNNGTG 1 cut(s) 3124
PaeR7I CTCGAG 1 cut(s) 2352
PceI AGGCCT 3 cut(s) 1253, 2718, 3151
PciI ACATGT 2 cut(s) 1396, 2424
PciSI GCTCTTC 1 cut(s) 1422
PdmI GAANNNNTTC 3 cut(s) 35, 422, 494
PfeI GAWTC 8 cut(s) 490, 526, 749, 796, 994, 1348, 1980, 2164
PflMI CCANNNNNTGG 1 cut(s) 2114
PfoI TCCNGGA 3 cut(s) 443, 485, 1729
PinAI ACCGGT 1 cut(s) 420
PkrI GCNGC 8 cut(s) 25, 242, 284, 734, 1632, 2143, 2384, 2887
PleI GAGTC 3 cut(s) 106, 1257, 1290
PpsI GAGTC 3 cut(s) 106, 1257, 1290
PpuMI RGGWCCY 2 cut(s) 364, 2762
PscI ACATGT 2 cut(s) 1396, 2424
PshAI GACNNNNGTC 1 cut(s) 1281
Psp124BI GAGCTC 3 cut(s) 108, 2359, 2854
Psp5II RGGWCCY 2 cut(s) 364, 2762
Psp6I CCWGG 9 cut(s) 170, 212, 1309, 1729, 1783, 2075, 2131, 2209, 2754
PspFI CCCAGC 1 cut(s) 658
PspGI CCWGG 9 cut(s) 170, 212, 1309, 1729, 1783, 2075, 2131, 2209, 2754
PspPPI RGGWCCY 2 cut(s) 364, 2762
PsrI GAACNNNNNNTAC 2 cut(s) 410, 442
PstI CTGCAG 2 cut(s) 2365, 2887
PstNI CAGNNNCTG 1 cut(s) 2765
PsuI RGATCY 4 cut(s) 135, 406, 928, 1772
PvuII CAGCTG 3 cut(s) 568, 2339, 2819
RsaI GTAC 7 cut(s) 1114, 1586, 2106, 2279, 2399, 2639, 2771
RsaNI GTAC 7 cut(s) 1113, 1585, 2105, 2278, 2398, 2638, 2770
RseI CAYNNNNRTG 3 cut(s) 1368, 2616, 3124
SacI GAGCTC 3 cut(s) 108, 2359, 2854
SapI GCTCTTC 1 cut(s) 1422
SatI GCNGC 8 cut(s) 24, 241, 283, 733, 1631, 2142, 2383, 2886
SchI GAGTC 3 cut(s) 107, 1257, 1291
SduI GDGCHC 7 cut(s) 108, 1022, 2359, 2424, 2627, 2854, 2869
SfaNI GCATC 3 cut(s) 2308, 2521, 3063
SfcI CTRYAG 4 cut(s) 899, 1269, 2361, 2883
Sfr274I CTCGAG 1 cut(s) 2352
SfuI TTCGAA 1 cut(s) 1820
SinI GGWCC 6 cut(s) 174, 216, 364, 515, 1302, 2762
SlaI CTCGAG 1 cut(s) 2352
SmaI CCCGGG 1 cut(s) 513
SmiMI CAYNNNNRTG 3 cut(s) 1368, 2616, 3124
SmlI CTYRAG 3 cut(s) 290, 1279, 2352
SmoI CTYRAG 3 cut(s) 290, 1279, 2352
SseBI AGGCCT 3 cut(s) 1253, 2718, 3151
SspMI CTAG 4 cut(s) 1770, 2315, 2411, 2553
SstI GAGCTC 3 cut(s) 108, 2359, 2854
StuI AGGCCT 3 cut(s) 1253, 2718, 3151
StyI CCWWGG 5 cut(s) 1387, 2386, 2481, 2552, 2725
TaaI ACNGT 8 cut(s) 35, 694, 1273, 2307, 2434, 2795, 2804, 2864
TaiI ACGT 2 cut(s) 1990, 2919
TaqII GACCGA 1 cut(s) 191
TatI WGTACW 2 cut(s) 2277, 2397
TauI GCSGC 3 cut(s) 243, 285, 735
TfiI GAWTC 8 cut(s) 490, 526, 749, 796, 994, 1348, 1980, 2164
TscAI CASTG 7 cut(s) 313, 322, 811, 967, 1303, 1335, 3126
TseFI GTSAC 2 cut(s) 698, 2969
TseI GCWGC 5 cut(s) 23, 1630, 2141, 2382, 2885
Tsp45I GTSAC 2 cut(s) 698, 2969
TspMI CCCGGG 1 cut(s) 511
TspRI CASTG 7 cut(s) 313, 322, 811, 967, 1303, 1335, 3126
Van91I CCANNNNNTGG 1 cut(s) 2114
Vha464I CTTAAG 1 cut(s) 290
VpaK11BI GGWCC 6 cut(s) 174, 216, 364, 515, 1302, 2762
XagI CCTNNNNNAGG 1 cut(s) 1386
XapI RAATTY 4 cut(s) 838, 1406, 1607, 2002
XbaI TCTAGA 1 cut(s) 2410
XceI RCATGY 3 cut(s) 1400, 2253, 2428
XhoI CTCGAG 1 cut(s) 2352
XmaI CCCGGG 1 cut(s) 511
XmaJI CCTAGG 1 cut(s) 2552
XmnI GAANNNNTTC 3 cut(s) 35, 422, 494
XspI CTAG 4 cut(s) 1770, 2315, 2411, 2553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.