AT5G16340

acyl-activating enzyme 5, peroxisomal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017051
Physical Location & Seq
Reverse (-)
5348993 .. 5351095
2103 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16340.1

Sequence Viewer

Length: 1653 bp
ATGGAGGAAATGAAGCCATGCGCCGCAAACTCGCCGCCGTTGACGCCGATAGGTTTTTTAGAGAGAGCCGCCACCGTTTACGGTGACTGTACCTCCATCGTTTATGGCAGCAACACCGTTTACACGTGGCGTGAAACAAACCTCCGTTGTCTCCGCGTGGCGTCTTCTCTGTCTTCAATCGGAATCGGCAGGTCTGACGTAGTCTCTGTTCTCTCTCCCAATACTCCGGCTATGTACGAGCTCCAGTTTGCTGTTCCCATGTCCGGCGCAATCCTCAACAACATCAACACACGCCTCGACGCACGCACCGTCTCTGTTCTTCTCCGTCACTGTGAATCTAAGCTTCTTTTCGTCGACGTCTTCTCCGTTGATCTCGCCGTTGAAGCGGTCTCGATGATGACGACTGATCCGCCGATTCTCGTCGTCATCGCCGATAAAGAAGAGGAAGGAGGAGTTGCTGACGTGGCTGATCTTTCAAAATTCAGTTACACTTACGATGATCTTATCGAAAGAGGTGATCCGGGTTTTAAATGGATCCGACCCGAAAGCGAATGGGATCCGGTTGTGCTTAATTACACTTCCGGTACGACTTCAGCTCCTAAAGGAGTGGTACACTGCCACAGAGGAATTTTCGTAATGTCAGTTGATTCACTAATTGATTGGGCCGTACCGAAAAATCCGGTTTACTTATGGACTCTACCGATATTTCACTCTAACGGTTGGACCAATCCATGGGGAATCGCCGCCGTCGGAGGAACTAACGTCTGTTTGCGTAAATTTGACGCGCCGTTAATCTACCGTCTGATCCGTGATCACGGCGTCACACACATGTGTGGAGCTCCGGTGGTGCTCAACATGTTATCCGCGACTCAGGAATCTCAGCCTCTTAACCATCCCGTCAACATCTTAACCGCCGGTTCTCCGCCTCCAGCTACCGTCCTCCTCCGAGCCGAATCAATTGGTTTCGTTATCAGTCACGGTTACGGATTAACGGAAACCGCCGGTGTAATCGTCTCGTGCGCGTGGAAGCCAAAATGGAATCATTTACCGGCAAGCGATCGAGCGAGATTGAAGGCACGGCAAGGAGTGAGAACCGTCGGGTTTACTGAAATCGACGTGGTGGATCCTGAATCGGGTTTGAGCGTTGAGAGAAATGGAGAAACTGTCGGAGAAATTGTGATGAGAGGAAGCTCGGTCATGCTCGGTTACTTAAAAGATCCGGTCGGAACAGAGAAAGCTTTAAAGAACGGGTGGTTTTACACCGGAGATGTTGGTGTGATTCATTCCGATGGTTATCTAGAGATTAAAGATAGATCGAAAGATATAATTATAACGGGAGGTGAGAATGTGAGTAGTGTTGAGGTTGAAACGGTGTTGTACACGATTCCGGCGGTGAATGAAGTGGCGGTGGTGGCTAGACCTGATGAGTTTTGGGGAGAGACACCGTGTGCGTTTGTGAGTTTGAAAAATGGGTTTAGTGGGAAACCTACGGAGGAGGAATTGATGGAGTATTGTAGAAAGAAGATGCCCAAGTATATGGTTCCTAAAACGGTGTCGTTTATGGATGAGTTGCCTAAGAGTTCGACGGGAAAGGTTACGAAGTTTGTGCTTAGGGACATTGCAAAGAAGATGGGTGATAAGACAATTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

550

Amino Acids

60.13

Weight (kDa)

5.61

Isoelectric Point (pI)

34.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 20 - 404 1.4e-75 AMP-binding enzyme
AMP-binding_C PF13193 454 - 531 1.4e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1331
AatII GACGTC 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 180
AccB7I CCANNNNNTGG 1 cut(s) 732
AccI GTMKAC 1 cut(s) 354
AccII CGCG 4 cut(s) 156, 785, 866, 1022
AcsI RAATTY 3 cut(s) 479, 627, 776
AcuI CTGAAG 1 cut(s) 576
AcvI CACGTG 1 cut(s) 126
AcyI GRCGYC 4 cut(s) 44, 161, 357, 819
AdeI CACNNNGTG 1 cut(s) 1448
AfaI GTAC 6 cut(s) 91, 236, 586, 612, 669, 1379
AfiI CCNNNNNNNGG 3 cut(s) 263, 732, 1133
AflIII ACRYGT 3 cut(s) 123, 828, 855
AgsI TTSAA 6 cut(s) 177, 383, 477, 1072, 1367, 1465
AjiI CACGTC 2 cut(s) 463, 1117
AleI CACNNNNGTG 2 cut(s) 829, 831
AluBI AGCT 7 cut(s) 241, 343, 596, 839, 932, 1191, 1238
AluI AGCT 7 cut(s) 241, 343, 596, 839, 932, 1191, 1238
Alw21I GWGCWC 3 cut(s) 243, 841, 852
Alw26I GTCTC 6 cut(s) 155, 208, 316, 394, 1018, 1433
AoxI GGCC 1 cut(s) 663
ApeKI GCWGC 1 cut(s) 108
ApoI RAATTY 3 cut(s) 479, 627, 776
AspLEI GCGC 4 cut(s) 23, 269, 787, 1022
AspS9I GGNCC 2 cut(s) 663, 723
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 5 cut(s) 95, 527, 1352, 1405, 1646
AvaII GGWCC 1 cut(s) 723
BamHI GGATCC 3 cut(s) 534, 556, 1123
BanII GRGCYC 2 cut(s) 243, 841
BauI CACGAG 1 cut(s) 1015
BbrPI CACGTG 1 cut(s) 126
BbsI GAAGAC 3 cut(s) 156, 165, 352
Bbv12I GWGCWC 3 cut(s) 243, 841, 852
BbvI GCAGC 1 cut(s) 120
BccI CCATC 5 cut(s) 104, 900, 1283, 1498, 1624
BceAI ACGGC 7 cut(s) 22, 362, 650, 731, 772, 832, 1094
BcgI CGANNNNNNTGC 2 cut(s) 1588, 1622
BclI TGATCA 1 cut(s) 811
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 6 cut(s) 155, 208, 316, 394, 1018, 1433
BfaI CTAG 2 cut(s) 1298, 1416
BfuAI ACCTGC 1 cut(s) 180
BisI GCNGC 5 cut(s) 24, 35, 69, 109, 744
BlsI GCNGC 5 cut(s) 25, 36, 70, 110, 745
Bme1390I CCNGG 1 cut(s) 522
Bme18I GGWCC 1 cut(s) 723
BmgBI CACGTC 2 cut(s) 463, 1117
BmgT120I GGNCC 2 cut(s) 663, 723
BmiI GGNNCC 4 cut(s) 536, 558, 1125, 1542
BmrFI CCNGG 1 cut(s) 522
BmsI GCATC 1 cut(s) 1515
BpiI GAAGAC 3 cut(s) 156, 165, 352
BpmI CTGGAG 2 cut(s) 227, 912
Bpu10I CCTNAGC 1 cut(s) 1610
BpuMI CCSGG 1 cut(s) 522
BsaAI YACGTR 1 cut(s) 126
BsaBI GATNNNNATC 1 cut(s) 1293
BsaHI GRCGYC 4 cut(s) 44, 161, 357, 819
BsaI GGTCTC 1 cut(s) 394
BsaJI CCNNGG 1 cut(s) 731
BsaWI WCCGGW 6 cut(s) 559, 581, 679, 841, 1219, 1262
Bsc4I CCNNNNNNNGG 3 cut(s) 263, 732, 1133
Bse118I RCCGGY 3 cut(s) 914, 1001, 1048
Bse1I ACTGG 1 cut(s) 244
Bse3DI GCAATG 1 cut(s) 1617
Bse8I GATNNNNATC 1 cut(s) 1293
BseDI CCNNGG 1 cut(s) 731
BseGI GGATG 2 cut(s) 892, 1570
BseJI GATNNNNATC 1 cut(s) 1293
BseLI CCNNNNNNNGG 3 cut(s) 263, 732, 1133
BseMI GCAATG 1 cut(s) 1617
BseMII CTCAG 2 cut(s) 884, 893
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 3 cut(s) 465, 932, 1508
BseXI GCAGC 1 cut(s) 120
Bsh1236I CGCG 4 cut(s) 156, 785, 866, 1022
Bsh1285I CGRYCG 2 cut(s) 1060, 1224
BshFI GGCC 1 cut(s) 665
BsiEI CGRYCG 2 cut(s) 1060, 1224
BsiHKAI GWGCWC 3 cut(s) 243, 841, 852
BslFI GGGAC 1 cut(s) 1628
BslI CCNNNNNNNGG 3 cut(s) 263, 732, 1133
BsmAI GTCTC 6 cut(s) 155, 208, 316, 394, 1018, 1433
BsmBI CGTCTC 2 cut(s) 316, 1018
BsmFI GGGAC 1 cut(s) 1628
BsnI GGCC 1 cut(s) 665
Bso31I GGTCTC 1 cut(s) 394
Bsp1286I GDGCHC 3 cut(s) 243, 841, 852
Bsp1407I TGTACA 1 cut(s) 1377
Bsp19I CCATGG 1 cut(s) 731
BspANI GGCC 1 cut(s) 665
BspCNI CTCAG 2 cut(s) 883, 892
BspFNI CGCG 4 cut(s) 156, 785, 866, 1022
BspLI GGNNCC 4 cut(s) 536, 558, 1125, 1542
BspMI ACCTGC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 394
BsrDI GCAATG 1 cut(s) 1617
BsrFI RCCGGY 3 cut(s) 914, 1001, 1048
BsrGI TGTACA 1 cut(s) 1377
BsrI ACTGG 1 cut(s) 244
BssAI RCCGGY 3 cut(s) 914, 1001, 1048
BssECI CCNNGG 1 cut(s) 731
BssNI GRCGYC 4 cut(s) 44, 161, 357, 819
BssSI CACGAG 1 cut(s) 1015
BssT1I CCWWGG 1 cut(s) 731
Bst2BI CACGAG 1 cut(s) 1015
Bst6I CTCTTC 1 cut(s) 435
BstACI GRCGYC 4 cut(s) 44, 161, 357, 819
BstAUI TGTACA 1 cut(s) 1377
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 304, 1054
BstDEI CTNAG 5 cut(s) 339, 870, 879, 1575, 1610
BstDSI CCRYGG 1 cut(s) 731
BstF5I GGATG 2 cut(s) 892, 1570
BstFNI CGCG 4 cut(s) 156, 785, 866, 1022
BstHHI GCGC 4 cut(s) 23, 269, 787, 1022
BstMAI GTCTC 6 cut(s) 155, 208, 316, 394, 1018, 1433
BstMCI CGRYCG 2 cut(s) 1060, 1224
BstMWI GCNNNNNNNGC 4 cut(s) 43, 383, 464, 1412
BstNSI RCATGY 2 cut(s) 832, 859
BstSCI CCNGG 1 cut(s) 520
BstUI CGCG 4 cut(s) 156, 785, 866, 1022
BstV1I GCAGC 1 cut(s) 120
BstV2I GAAGAC 3 cut(s) 156, 165, 352
BstX2I RGATCY 4 cut(s) 534, 556, 1123, 1216
BstXI CCANNNNNNTGG 1 cut(s) 1537
BstYI RGATCY 4 cut(s) 534, 556, 1123, 1216
BsuRI GGCC 1 cut(s) 665
BtgI CCRYGG 1 cut(s) 731
BtgZI GCGATG 1 cut(s) 412
BtrI CACGTC 2 cut(s) 463, 1117
BtsCI GGATG 2 cut(s) 892, 1570
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 2 cut(s) 328, 613
BveI ACCTGC 1 cut(s) 180
Cac8I GCNNGC 2 cut(s) 304, 1054
CfoI GCGC 4 cut(s) 23, 269, 787, 1022
Cfr10I RCCGGY 3 cut(s) 914, 1001, 1048
Cfr13I GGNCC 2 cut(s) 663, 723
CseI GACGC 5 cut(s) 52, 150, 308, 791, 808
Csp6I GTAC 6 cut(s) 90, 235, 585, 611, 668, 1378
CviAII CATG 6 cut(s) 18, 259, 732, 829, 856, 1198
CviQI GTAC 6 cut(s) 90, 235, 585, 611, 668, 1378
DdeI CTNAG 5 cut(s) 339, 870, 879, 1575, 1610
DraI TTTAAA 2 cut(s) 529, 1242
DraIII CACNNNGTG 1 cut(s) 1448
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
EciI GGCGGA 2 cut(s) 399, 912
Ecl136II GAGCTC 2 cut(s) 241, 839
Eco130I CCWWGG 1 cut(s) 731
Eco24I GRGCYC 2 cut(s) 243, 841
Eco31I GGTCTC 1 cut(s) 394
Eco47I GGWCC 1 cut(s) 723
Eco53kI GAGCTC 2 cut(s) 241, 839
Eco57I CTGAAG 1 cut(s) 576
Eco72I CACGTG 1 cut(s) 126
EcoICRI GAGCTC 2 cut(s) 241, 839
EcoT14I CCWWGG 1 cut(s) 731
EcoT38I GRGCYC 2 cut(s) 243, 841
ErhI CCWWGG 1 cut(s) 731
Esp3I CGTCTC 2 cut(s) 316, 1018
FaeI CATG 6 cut(s) 21, 262, 735, 832, 859, 1201
FaqI GGGAC 1 cut(s) 1628
FatI CATG 6 cut(s) 17, 258, 731, 828, 855, 1197
FbaI TGATCA 1 cut(s) 811
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 5 cut(s) 24, 35, 69, 109, 744
FokI GGATG 2 cut(s) 879, 1577
FriOI GRGCYC 2 cut(s) 243, 841
Fsp4HI GCNGC 5 cut(s) 24, 35, 69, 109, 744
FspBI CTAG 2 cut(s) 1298, 1416
GlaI GCGC 4 cut(s) 22, 268, 786, 1021
GluI GCNGC 5 cut(s) 24, 35, 69, 109, 744
GsuI CTGGAG 2 cut(s) 227, 912
HaeIII GGCC 1 cut(s) 665
HgaI GACGC 5 cut(s) 52, 150, 308, 791, 808
HhaI GCGC 4 cut(s) 23, 269, 787, 1022
Hin1I GRCGYC 4 cut(s) 44, 161, 357, 819
Hin1II CATG 6 cut(s) 21, 262, 735, 832, 859, 1201
Hin6I GCGC 4 cut(s) 21, 267, 785, 1020
HinP1I GCGC 4 cut(s) 21, 267, 785, 1020
HincII GTYRAC 3 cut(s) 42, 355, 901
HindII GTYRAC 3 cut(s) 42, 355, 901
HindIII AAGCTT 2 cut(s) 341, 1236
HphI GGTGA 5 cut(s) 95, 527, 1352, 1405, 1646
Hpy166II GTNNAC 9 cut(s) 42, 79, 121, 355, 613, 685, 901, 1104, 1380
Hpy188I TCNGA 9 cut(s) 182, 196, 539, 752, 804, 947, 1169, 1226, 1288
Hpy188III TCNNGA 4 cut(s) 391, 872, 1127, 1298
Hpy8I GTNNAC 9 cut(s) 42, 79, 121, 355, 613, 685, 901, 1104, 1380
Hpy99I CGWCG 8 cut(s) 302, 356, 359, 425, 752, 1100, 1118, 1588
HpyAV CCTTC 2 cut(s) 440, 1066
HpyCH4IV ACGT 6 cut(s) 125, 198, 357, 462, 762, 1116
HpyCH4V TGCA 1 cut(s) 1622
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 383, 464, 1412
HpyF3I CTNAG 5 cut(s) 339, 870, 879, 1575, 1610
HpySE526I ACGT 6 cut(s) 125, 198, 357, 462, 762, 1116
Hsp92I GRCGYC 4 cut(s) 44, 161, 357, 819
Hsp92II CATG 6 cut(s) 21, 262, 735, 832, 859, 1201
HspAI GCGC 4 cut(s) 21, 267, 785, 1020
Ksp22I TGATCA 1 cut(s) 811
LmnI GCTCC 4 cut(s) 246, 601, 836, 844
Lsp1109I GCAGC 1 cut(s) 120
LweI GCATC 1 cut(s) 1515
MaeI CTAG 2 cut(s) 1298, 1416
MaeII ACGT 6 cut(s) 125, 198, 357, 462, 762, 1116
MaeIII GTNAC 8 cut(s) 83, 326, 485, 820, 974, 980, 1205, 1594
MboII GAAGA 7 cut(s) 156, 165, 311, 352, 452, 1534, 1639
MfeI CAATTG 1 cut(s) 957
MflI RGATCY 4 cut(s) 534, 556, 1123, 1216
MhlI GDGCHC 3 cut(s) 243, 841, 852
MlyI GAGTC 2 cut(s) 688, 862
MmeI TCCRAC 5 cut(s) 562, 701, 730, 1147, 1204
MslI CAYNNNNRTG 4 cut(s) 827, 829, 831, 1287
MspR9I CCNGG 1 cut(s) 522
MunI CAATTG 1 cut(s) 957
MvnI CGCG 4 cut(s) 156, 785, 866, 1022
MwoI GCNNNNNNNGC 4 cut(s) 43, 383, 464, 1412
NciI CCSGG 1 cut(s) 522
NcoI CCATGG 1 cut(s) 731
NlaIII CATG 6 cut(s) 21, 262, 735, 832, 859, 1201
NlaIV GGNNCC 4 cut(s) 536, 558, 1125, 1542
NmuCI GTSAC 4 cut(s) 83, 326, 820, 974
NspI RCATGY 2 cut(s) 832, 859
OliI CACNNNNGTG 2 cut(s) 829, 831
PciI ACATGT 2 cut(s) 828, 855
PcsI WCGNNNNNNNCGW 4 cut(s) 306, 363, 398, 429
PflFI GACNNNGTC 1 cut(s) 200
PflMI CCANNNNNTGG 1 cut(s) 732
PkrI GCNGC 5 cut(s) 25, 36, 70, 110, 745
Ple19I CGATCG 1 cut(s) 1060
PleI GAGTC 2 cut(s) 688, 862
PmaCI CACGTG 1 cut(s) 126
PmlI CACGTG 1 cut(s) 126
PpsI GAGTC 2 cut(s) 688, 862
Ppu21I YACGTR 1 cut(s) 126
PscI ACATGT 2 cut(s) 828, 855
PsiI TTATAA 1 cut(s) 1331
Psp124BI GAGCTC 2 cut(s) 243, 841
PspCI CACGTG 1 cut(s) 126
PspN4I GGNNCC 4 cut(s) 536, 558, 1125, 1542
PspPI GGNCC 2 cut(s) 663, 723
PsrI GAACNNNNNNTAC 2 cut(s) 192, 224
PsuI RGATCY 4 cut(s) 534, 556, 1123, 1216
PsyI GACNNNGTC 1 cut(s) 200
PvuI CGATCG 1 cut(s) 1060
RsaI GTAC 6 cut(s) 91, 236, 586, 612, 669, 1379
RsaNI GTAC 6 cut(s) 90, 235, 585, 611, 668, 1378
RseI CAYNNNNRTG 4 cut(s) 827, 829, 831, 1287
SacI GAGCTC 2 cut(s) 243, 841
SalI GTCGAC 1 cut(s) 353
SatI GCNGC 5 cut(s) 24, 35, 69, 109, 744
Sau96I GGNCC 2 cut(s) 663, 723
SchI GAGTC 2 cut(s) 688, 862
ScrFI CCNGG 1 cut(s) 522
SduI GDGCHC 3 cut(s) 243, 841, 852
SfaNI GCATC 1 cut(s) 1515
SgrAI CRCCGGYG 1 cut(s) 1001
SgrDI CGTCGACG 1 cut(s) 353
SinI GGWCC 1 cut(s) 723
SmiMI CAYNNNNRTG 4 cut(s) 827, 829, 831, 1287
SspMI CTAG 2 cut(s) 1298, 1416
SstI GAGCTC 2 cut(s) 243, 841
StyD4I CCNGG 1 cut(s) 520
StyI CCWWGG 1 cut(s) 731
TaiI ACGT 6 cut(s) 128, 201, 360, 465, 765, 1119
TaqI TCGA 8 cut(s) 297, 354, 392, 507, 1060, 1113, 1316, 1583
TaqII GACCGA 1 cut(s) 1183
TatI WGTACW 1 cut(s) 1377
TauI GCSGC 4 cut(s) 26, 37, 71, 746
TscAI CASTG 2 cut(s) 335, 620
TseFI GTSAC 4 cut(s) 83, 326, 820, 974
TseI GCWGC 1 cut(s) 108
Tsp45I GTSAC 4 cut(s) 83, 326, 820, 974
TspDTI ATGAA 3 cut(s) 26, 1271, 1413
TspGWI ACGGA 7 cut(s) 134, 314, 355, 797, 999, 1007, 1505
TspRI CASTG 2 cut(s) 335, 620
Tth111I GACNNNGTC 1 cut(s) 200
Van91I CCANNNNNTGG 1 cut(s) 732
VpaK11BI GGWCC 1 cut(s) 723
XapI RAATTY 3 cut(s) 479, 627, 776
XbaI TCTAGA 1 cut(s) 1297
XceI RCATGY 2 cut(s) 832, 859
XmiI GTMKAC 1 cut(s) 354
XspI CTAG 2 cut(s) 1298, 1416
ZraI GACGTC 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.