Rh6DG138900

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0017051
Physical Location & Seq
Reverse (-)
19992798 .. 19993301
504 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG138900.1

Sequence Viewer

Length: 504 bp
ATGGAAAGTGTACCGCCTGATGGTAAGACAATGGGTGAAATTATGTTCAGGGGAAACACTGTGATGAGTGGTTATCTTAAAGACTCGAGAGCAACAGAGGAAGCTTTTAAGGGTGGATGGTTTAGAAGTGGGGATTTAGCTGTGAAACATCCTGATAACTATATCGAAGTCAAGGACCGGTTGAAGGATGTGATTATATCTAGGGGAGAGAACATAAGCACAGTTGAGGTTGAAACAGTTTTGTTTAGCCATCCAGCAGTTCTTGAGGCTGCAGTTGTTGCAAGGCCTGATAATCATTGGGGACAAACTCCTTATGCATTTGTGAAATTGAAGGACGGCTTTGATGTGAATGCACAAGAACTAATTGAGTTCTGCCGCAATCATATGCCTCATTACATGGCACCCCGGACAGTCATTTTTGATGATATACCAAAGACTTCAACAGGAAAGATACAAAAATTCATTCTCAGAGAAAAAGCAAAAGTCTTGGGCAGCCTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

167

Amino Acids

18.77

Weight (kDa)

6.52

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding_C PF13193 76 - 150 2.1e-22 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 400
AciI CCGC 2 cut(s) 14, 376
AcsI RAATTY 1 cut(s) 458
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 2 cut(s) 20, 184
AgeI ACCGGT 1 cut(s) 177
AgsI TTSAA 4 cut(s) 184, 233, 331, 441
AluBI AGCT 2 cut(s) 104, 140
AluI AGCT 2 cut(s) 104, 140
Ama87I CYCGRG 1 cut(s) 85
AoxI GGCC 1 cut(s) 284
ApeKI GCWGC 2 cut(s) 269, 492
ApoI RAATTY 1 cut(s) 458
AsiGI ACCGGT 1 cut(s) 177
AspS9I GGNCC 1 cut(s) 175
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 1 cut(s) 47
AvaI CYCGRG 1 cut(s) 85
AvaII GGWCC 1 cut(s) 175
BanI GGYRCC 1 cut(s) 400
BbvI GCAGC 1 cut(s) 256
BccI CCATC 3 cut(s) 14, 111, 258
BceAI ACGGC 1 cut(s) 352
BcnI CCSGG 1 cut(s) 406
BfaI CTAG 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 270
BisI GCNGC 3 cut(s) 270, 376, 493
BlsI GCNGC 3 cut(s) 271, 377, 494
Bme1390I CCNGG 1 cut(s) 406
Bme18I GGWCC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 402
BmrFI CCNGG 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 284
BpuMI CCSGG 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 404
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 184
Bse118I RCCGGY 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 404
BseGI GGATG 4 cut(s) 122, 148, 193, 250
BseLI CCNNNNNNNGG 2 cut(s) 20, 184
BseMII CTCAG 1 cut(s) 481
BseXI GCAGC 1 cut(s) 256
BshFI GGCC 1 cut(s) 286
BshNI GGYRCC 1 cut(s) 400
BshTI ACCGGT 1 cut(s) 177
BsiHKCI CYCGRG 1 cut(s) 85
BsiSI CCGG 2 cut(s) 178, 406
BslFI GGGAC 1 cut(s) 315
BslI CCNNNNNNNGG 2 cut(s) 20, 184
BsmFI GGGAC 1 cut(s) 315
BsmI GAATGC 1 cut(s) 355
BsnI GGCC 1 cut(s) 286
BsoBI CYCGRG 1 cut(s) 85
BspACI CCGC 2 cut(s) 14, 376
BspANI GGCC 1 cut(s) 286
BspCNI CTCAG 1 cut(s) 480
BspLI GGNNCC 1 cut(s) 402
BspMAI CTGCAG 1 cut(s) 274
BspT107I GGYRCC 1 cut(s) 400
BsrFI RCCGGY 1 cut(s) 177
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 404
Bst4CI ACNGT 4 cut(s) 61, 223, 238, 412
BstAPI GCANNNNNTGC 1 cut(s) 278
BstDEI CTNAG 1 cut(s) 467
BstF5I GGATG 4 cut(s) 122, 148, 193, 250
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 404
BstSFI CTRYAG 1 cut(s) 270
BstV1I GCAGC 1 cut(s) 256
BsuRI GGCC 1 cut(s) 286
BtsCI GGATG 4 cut(s) 122, 148, 193, 250
BtsIMutI CAGTG 1 cut(s) 57
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 175
Csp6I GTAC 1 cut(s) 11
CspAI ACCGGT 1 cut(s) 177
CviAII CATG 1 cut(s) 397
CviJI RGCY 7 cut(s) 104, 140, 249, 269, 286, 339, 495
CviKI_1 RGCY 7 cut(s) 104, 140, 249, 269, 286, 339, 495
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 1 cut(s) 467
Eco147I AGGCCT 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 175
Eco88I CYCGRG 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 319
FaeI CATG 1 cut(s) 400
FaiI YATR 9 cut(s) 44, 162, 197, 215, 315, 384, 386, 398, 428
FalI AAGNNNNNCTT 2 cut(s) 323, 355
FaqI GGGAC 1 cut(s) 315
FatI CATG 1 cut(s) 396
FauNDI CATATG 1 cut(s) 384
Fnu4HI GCNGC 3 cut(s) 270, 376, 493
FokI GGATG 4 cut(s) 129, 135, 200, 237
Fsp4HI GCNGC 3 cut(s) 270, 376, 493
FspBI CTAG 1 cut(s) 201
GluI GCNGC 3 cut(s) 270, 376, 493
HaeIII GGCC 1 cut(s) 286
HapII CCGG 2 cut(s) 178, 406
Hin1II CATG 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 102
HinfI GANTC 1 cut(s) 83
HpaII CCGG 2 cut(s) 178, 406
HphI GGTGA 1 cut(s) 47
Hpy166II GTNNAC 1 cut(s) 11
Hpy188I TCNGA 1 cut(s) 470
Hpy188III TCNNGA 4 cut(s) 87, 152, 263, 501
Hpy8I GTNNAC 1 cut(s) 11
HpyAV CCTTC 2 cut(s) 178, 325
HpyCH4III ACNGT 4 cut(s) 61, 223, 238, 412
HpyCH4V TGCA 4 cut(s) 272, 281, 317, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 1 cut(s) 467
Hsp92II CATG 1 cut(s) 400
LpnPI CCDG 8 cut(s) 30, 34, 165, 191, 267, 300, 419, 429
Lsp1109I GCAGC 1 cut(s) 256
MaeI CTAG 1 cut(s) 201
MluCI AATT 4 cut(s) 39, 326, 363, 458
MlyI GAGTC 1 cut(s) 77
MnlI CCTC 4 cut(s) 91, 220, 259, 399
Mph1103I ATGCAT 1 cut(s) 319
MseI TTAA 2 cut(s) 78, 108
MslI CAYNNNNRTG 1 cut(s) 62
MspI CCGG 2 cut(s) 178, 406
MspR9I CCNGG 1 cut(s) 406
Mva1269I GAATGC 1 cut(s) 355
MwoI GCNNNNNNNGC 1 cut(s) 278
NciI CCSGG 1 cut(s) 406
NdeI CATATG 1 cut(s) 384
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 1 cut(s) 402
NsiI ATGCAT 1 cut(s) 319
PaeR7I CTCGAG 1 cut(s) 85
PceI AGGCCT 1 cut(s) 286
PctI GAATGC 1 cut(s) 355
PinAI ACCGGT 1 cut(s) 177
PkrI GCNGC 3 cut(s) 271, 377, 494
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
PspN4I GGNNCC 1 cut(s) 402
PspPI GGNCC 1 cut(s) 175
PstI CTGCAG 1 cut(s) 274
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
RseI CAYNNNNRTG 1 cut(s) 62
SaqAI TTAA 2 cut(s) 78, 108
SatI GCNGC 3 cut(s) 270, 376, 493
Sau96I GGNCC 1 cut(s) 175
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 1 cut(s) 406
SetI ASST 3 cut(s) 106, 142, 231
SfcI CTRYAG 1 cut(s) 270
Sfr274I CTCGAG 1 cut(s) 85
SinI GGWCC 1 cut(s) 175
SlaI CTCGAG 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 2 cut(s) 85, 263
SmoI CTYRAG 2 cut(s) 85, 263
Sse9I AATT 4 cut(s) 39, 326, 363, 458
SseBI AGGCCT 1 cut(s) 286
SsiI CCGC 2 cut(s) 14, 376
SspMI CTAG 1 cut(s) 201
StuI AGGCCT 1 cut(s) 286
StyD4I CCNGG 1 cut(s) 404
TaaI ACNGT 4 cut(s) 61, 223, 238, 412
TaqI TCGA 2 cut(s) 86, 165
TasI AATT 4 cut(s) 39, 326, 363, 458
TauI GCSGC 1 cut(s) 378
Tru1I TTAA 2 cut(s) 78, 108
Tru9I TTAA 2 cut(s) 78, 108
TscAI CASTG 1 cut(s) 64
TseI GCWGC 2 cut(s) 269, 492
TspDTI ATGAA 1 cut(s) 451
TspRI CASTG 1 cut(s) 64
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 1 cut(s) 458
XhoI CTCGAG 1 cut(s) 85
XspI CTAG 1 cut(s) 201
Zsp2I ATGCAT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.