AT5G16370

acyl-activating enzyme 5, peroxisomal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017051
Physical Location & Seq
Reverse (-)
5356551 .. 5358789
2239 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16370.1

Sequence Viewer

Length: 1659 bp
ATGGAGCAAATGAAGCCATGCGCCGCAAACTCGCCGCCGTTGACGCCGATAGGTTTCTTAGAGAGAGCCGCCACCGTTTACGGTGACTGTACCTCCATCGTTTATGGCAGCAACACCGTTTACACGTGGCGTGAAACAAACCTCCGTTGTCTCCGCGTGGCGTCTTCTCTGTCTTCAATCGGAATCGGCAGGTCTGACGTAGTCTCTGTTCTCTCTCCCAATACTCCGGCTATGTACGAGCTCCAGTTTGCTGTTCCCATGTCCGGCGCAATCCTCAACAACATCAACACTCGCCTCGACGCACGCACCGTCTCTGTTCTTCTCCGTCACTGTGGATCTAAGCTTCTCTTCGTCGACGTCTTCTCCGTTGATCTTGCCGTTGAAGCGATCTCGATGATGACGACTGATCCGCCGATTCTTGTCTTCATCGCCGATAAAGAAGAAGAAGGAGGAGATGCTGACGTGGCGGATCGTACCAAATTCAGTTACACTTACGATGATCTGATCCATAGAGGTGATCTGGATTTTAAATGGATCCGACCCGAAAGCGAATGGGATCCGGTTGTGCTTAATTACACTTCCGGTACGACTTCGGCTCCTAAAGGAGTCGTACACTGCCACAGAGGAATTTTCGTAATGTCAATTGATTCTTTAATCGATTGGACCGTACCGAAAAATCCGGTTTACTTATGGACTCTACCGATATTTCACGCTAACGGCTGGAGCTATCCATGGGGAATCGCCGCCGTCGGAGGAACTAACGTCTGTTTGCGTAAATTCGACGCGCCGTTAATCTACCGTTTGATCCGTGATCACGGCGTCACACACATGTGTGGAGCTCCGGTAGTGCTCAACATGTTGTCGGCGACTAACGAATTTCAGCCGTTAAATCGTCCTGTCAACATCTTAACCGCCGGTGCTCCGCCTCCAGCAGCCGTACTCCTCCGAGCAGAATCAATTGGATTCGTGATAAGTCACGGATACGGGTTAACGGAAACCGCCGGATTAAACGTGTCATGCGCGTGGAAGCCACAGTGGAATCGTTTACCGGCGAGCGATCGAGCGAGGTTGAAAGCACGGCAAGGAGTGAGAACCGTCGGATTTACTGAAATCGACGTGGTGGATCCTGAATCAGGTAGGAGCGTTGAGAGAAACGGAGAAACCGTCGGAGAAATAGTGATGAGAGGAAGCTCGATCATGCTCGGTTACTTAAAAGATCCGGTCGGAACAGAGAAAGCTTTAAAGAACGGGTGGTTTTACACCGGAGATGTTGGTGTGATTCATTCCGATGGTTATCTAGAGATTAAAGATAGATCGAAAGATATAATTATAACGGGAGGTGAAAATGTGAGTAGTGTTGAGGTTGAAACGGTTTTGTATACGAATCCGGCGGTGAATGAAGTGGCGGTGGTGGCGAGACCTGATGTGTTTTGGGGAGAGACGCCGTGTGCGTTTGTTAGTTTGAAAAGTGGGTTGACTCAAAGACCGACGGAGGTGGAAATGATAGAGTATTGTAGGAAGAAGATGCCGAAATATATGGTTCCTAAAACGGTGTCCTTTGTGGATGAGCTGCCTAAAACTTCGACGGGGAAGGTTATGAAGTTTGTACTTAGAGAGATTGCGAAGAAGATGGGTACGACGAGGTTGAGTCGGATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

552

Amino Acids

60.77

Weight (kDa)

6.53

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 20 - 404 5.7e-75 AMP-binding enzyme
AMP-binding_C PF13193 454 - 531 3.4e-24 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1331
AatII GACGTC 1 cut(s) 360
Acc36I ACCTGC 1 cut(s) 180
AccI GTMKAC 2 cut(s) 354, 1379
AccII CGCG 3 cut(s) 156, 785, 1022
AcsI RAATTY 4 cut(s) 479, 627, 776, 875
AcvI CACGTG 1 cut(s) 126
AcyI GRCGYC 5 cut(s) 44, 161, 357, 819, 1442
AfaI GTAC 9 cut(s) 91, 236, 475, 586, 612, 669, 939, 1608, 1636
AfiI CCNNNNNNNGG 2 cut(s) 263, 1133
AflIII ACRYGT 4 cut(s) 123, 828, 855, 1011
AgsI TTSAA 5 cut(s) 177, 383, 1072, 1367, 1465
AjiI CACGTC 2 cut(s) 463, 1117
AleI CACNNNNGTG 2 cut(s) 829, 831
AluBI AGCT 7 cut(s) 241, 343, 726, 839, 1191, 1238, 1570
AluI AGCT 7 cut(s) 241, 343, 726, 839, 1191, 1238, 1570
Alw21I GWGCWC 4 cut(s) 243, 841, 852, 922
Alw26I GTCTC 5 cut(s) 155, 208, 316, 1411, 1433
ApeKI GCWGC 3 cut(s) 108, 932, 1570
ApoI RAATTY 4 cut(s) 479, 627, 776, 875
AspLEI GCGC 4 cut(s) 23, 269, 787, 1022
AspS9I GGNCC 1 cut(s) 663
AsuHPI GGTGA 4 cut(s) 95, 527, 1352, 1405
AvaII GGWCC 1 cut(s) 663
BamHI GGATCC 3 cut(s) 534, 556, 1123
BanII GRGCYC 2 cut(s) 243, 841
BbrPI CACGTG 1 cut(s) 126
BbsI GAAGAC 4 cut(s) 156, 165, 352, 415
Bbv12I GWGCWC 4 cut(s) 243, 841, 852, 922
BbvI GCAGC 3 cut(s) 120, 944, 1557
BccI CCATC 3 cut(s) 104, 1283, 1624
BciVI GTATCC 1 cut(s) 974
BclI TGATCA 1 cut(s) 811
BcoDI GTCTC 5 cut(s) 155, 208, 316, 1411, 1433
BfaI CTAG 1 cut(s) 1298
BfuAI ACCTGC 1 cut(s) 180
BfuI GTATCC 1 cut(s) 974
BisI GCNGC 7 cut(s) 24, 35, 69, 109, 744, 933, 1571
BlsI GCNGC 7 cut(s) 25, 36, 70, 110, 745, 934, 1572
Bme18I GGWCC 1 cut(s) 663
BmgBI CACGTC 2 cut(s) 463, 1117
BmgT120I GGNCC 1 cut(s) 663
BmiI GGNNCC 5 cut(s) 536, 558, 597, 1125, 1542
BmsI GCATC 2 cut(s) 445, 1515
BpiI GAAGAC 4 cut(s) 156, 165, 352, 415
BpmI CTGGAG 3 cut(s) 227, 742, 912
Bsa29I ATCGAT 1 cut(s) 657
BsaAI YACGTR 1 cut(s) 126
BsaBI GATNNNNATC 1 cut(s) 1293
BsaHI GRCGYC 5 cut(s) 44, 161, 357, 819, 1442
BsaI GGTCTC 1 cut(s) 1411
BsaJI CCNNGG 1 cut(s) 731
BsaWI WCCGGW 6 cut(s) 559, 581, 679, 841, 1219, 1262
Bsc4I CCNNNNNNNGG 2 cut(s) 263, 1133
Bse118I RCCGGY 2 cut(s) 914, 1048
Bse1I ACTGG 1 cut(s) 244
Bse8I GATNNNNATC 1 cut(s) 1293
BseCI ATCGAT 1 cut(s) 657
BseDI CCNNGG 1 cut(s) 731
BseGI GGATG 2 cut(s) 1570, 1659
BseJI GATNNNNATC 1 cut(s) 1293
BseLI CCNNNNNNNGG 2 cut(s) 263, 1133
BseNI ACTGG 1 cut(s) 244
BseRI GAGGAG 2 cut(s) 465, 932
BseXI GCAGC 3 cut(s) 120, 944, 1557
Bsh1236I CGCG 3 cut(s) 156, 785, 1022
Bsh1285I CGRYCG 2 cut(s) 1060, 1224
BshVI ATCGAT 1 cut(s) 657
BsiEI CGRYCG 2 cut(s) 1060, 1224
BsiHKAI GWGCWC 4 cut(s) 243, 841, 852, 922
BslI CCNNNNNNNGG 2 cut(s) 263, 1133
BsmAI GTCTC 5 cut(s) 155, 208, 316, 1411, 1433
BsmBI CGTCTC 2 cut(s) 316, 1433
Bso31I GGTCTC 1 cut(s) 1411
Bsp1286I GDGCHC 4 cut(s) 243, 841, 852, 922
Bsp19I CCATGG 1 cut(s) 731
BspDI ATCGAT 1 cut(s) 657
BspFNI CGCG 3 cut(s) 156, 785, 1022
BspLI GGNNCC 5 cut(s) 536, 558, 597, 1125, 1542
BspMI ACCTGC 1 cut(s) 180
BspTNI GGTCTC 1 cut(s) 1411
BsrFI RCCGGY 2 cut(s) 914, 1048
BsrI ACTGG 1 cut(s) 244
BssAI RCCGGY 2 cut(s) 914, 1048
BssECI CCNNGG 1 cut(s) 731
BssNAI GTATAC 1 cut(s) 1380
BssNI GRCGYC 5 cut(s) 44, 161, 357, 819, 1442
BssT1I CCWWGG 1 cut(s) 731
Bst1107I GTATAC 1 cut(s) 1380
Bst6I CTCTTC 1 cut(s) 353
BstACI GRCGYC 5 cut(s) 44, 161, 357, 819, 1442
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 304, 1054
BstDEI CTNAG 3 cut(s) 58, 339, 1610
BstDSI CCRYGG 1 cut(s) 731
BstENI CCTNNNNNAGG 1 cut(s) 1131
BstF5I GGATG 2 cut(s) 1570, 1659
BstFNI CGCG 3 cut(s) 156, 785, 1022
BstHHI GCGC 4 cut(s) 23, 269, 787, 1022
BstMAI GTCTC 5 cut(s) 155, 208, 316, 1411, 1433
BstMCI CGRYCG 2 cut(s) 1060, 1224
BstMWI GCNNNNNNNGC 5 cut(s) 13, 43, 383, 464, 1412
BstNSI RCATGY 2 cut(s) 832, 859
BstUI CGCG 3 cut(s) 156, 785, 1022
BstV1I GCAGC 3 cut(s) 120, 944, 1557
BstV2I GAAGAC 4 cut(s) 156, 165, 352, 415
BstX2I RGATCY 5 cut(s) 335, 534, 556, 1123, 1216
BstYI RGATCY 5 cut(s) 335, 534, 556, 1123, 1216
BstZ17I GTATAC 1 cut(s) 1380
Bsu15I ATCGAT 1 cut(s) 657
BsuI GTATCC 1 cut(s) 974
BsuTUI ATCGAT 1 cut(s) 657
BtgI CCRYGG 1 cut(s) 731
BtgZI GCGATG 1 cut(s) 412
BtrI CACGTC 2 cut(s) 463, 1117
BtsCI GGATG 2 cut(s) 1570, 1659
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 3 cut(s) 328, 613, 1040
BveI ACCTGC 1 cut(s) 180
Cac8I GCNNGC 2 cut(s) 304, 1054
CfoI GCGC 4 cut(s) 23, 269, 787, 1022
Cfr10I RCCGGY 2 cut(s) 914, 1048
Cfr13I GGNCC 1 cut(s) 663
ClaI ATCGAT 1 cut(s) 657
CseI GACGC 6 cut(s) 52, 150, 308, 791, 808, 1450
Csp6I GTAC 9 cut(s) 90, 235, 474, 585, 611, 668, 938, 1607, 1635
CviAII CATG 7 cut(s) 18, 259, 732, 829, 856, 1017, 1198
CviQI GTAC 9 cut(s) 90, 235, 474, 585, 611, 668, 938, 1607, 1635
DdeI CTNAG 3 cut(s) 58, 339, 1610
DraI TTTAAA 2 cut(s) 529, 1242
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
EciI GGCGGA 3 cut(s) 399, 482, 912
Ecl136II GAGCTC 2 cut(s) 241, 839
Eco130I CCWWGG 1 cut(s) 731
Eco24I GRGCYC 2 cut(s) 243, 841
Eco31I GGTCTC 1 cut(s) 1411
Eco47I GGWCC 1 cut(s) 663
Eco53kI GAGCTC 2 cut(s) 241, 839
Eco72I CACGTG 1 cut(s) 126
EcoICRI GAGCTC 2 cut(s) 241, 839
EcoNI CCTNNNNNAGG 1 cut(s) 1131
EcoT14I CCWWGG 1 cut(s) 731
EcoT38I GRGCYC 2 cut(s) 243, 841
ErhI CCWWGG 1 cut(s) 731
Esp3I CGTCTC 2 cut(s) 316, 1433
FaeI CATG 7 cut(s) 21, 262, 735, 832, 859, 1020, 1201
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FatI CATG 7 cut(s) 17, 258, 731, 828, 855, 1016, 1197
FbaI TGATCA 1 cut(s) 811
FblI GTMKAC 2 cut(s) 354, 1379
Fnu4HI GCNGC 7 cut(s) 24, 35, 69, 109, 744, 933, 1571
FokI GGATG 1 cut(s) 1577
FriOI GRGCYC 2 cut(s) 243, 841
Fsp4HI GCNGC 7 cut(s) 24, 35, 69, 109, 744, 933, 1571
FspBI CTAG 1 cut(s) 1298
GlaI GCGC 4 cut(s) 22, 268, 786, 1021
GluI GCNGC 7 cut(s) 24, 35, 69, 109, 744, 933, 1571
GsuI CTGGAG 3 cut(s) 227, 742, 912
HgaI GACGC 6 cut(s) 52, 150, 308, 791, 808, 1450
HhaI GCGC 4 cut(s) 23, 269, 787, 1022
Hin1I GRCGYC 5 cut(s) 44, 161, 357, 819, 1442
Hin1II CATG 7 cut(s) 21, 262, 735, 832, 859, 1020, 1201
Hin6I GCGC 4 cut(s) 21, 267, 785, 1020
HinP1I GCGC 4 cut(s) 21, 267, 785, 1020
HincII GTYRAC 5 cut(s) 42, 355, 901, 990, 1476
HindII GTYRAC 5 cut(s) 42, 355, 901, 990, 1476
HindIII AAGCTT 2 cut(s) 341, 1236
HpaI GTTAAC 1 cut(s) 990
HphI GGTGA 4 cut(s) 95, 527, 1352, 1405
Hpy188III TCNNGA 5 cut(s) 391, 521, 967, 1127, 1298
HpyAV CCTTC 2 cut(s) 440, 1585
HpyCH4IV ACGT 7 cut(s) 125, 198, 357, 462, 762, 1011, 1116
HpyF10VI GCNNNNNNNGC 5 cut(s) 13, 43, 383, 464, 1412
HpyF3I CTNAG 3 cut(s) 58, 339, 1610
HpySE526I ACGT 7 cut(s) 125, 198, 357, 462, 762, 1011, 1116
Hsp92I GRCGYC 5 cut(s) 44, 161, 357, 819, 1442
Hsp92II CATG 7 cut(s) 21, 262, 735, 832, 859, 1020, 1201
HspAI GCGC 4 cut(s) 21, 267, 785, 1020
Ksp22I TGATCA 1 cut(s) 811
KspAI GTTAAC 1 cut(s) 990
LmnI GCTCC 8 cut(s) 4, 246, 601, 723, 836, 844, 925, 1140
Lsp1109I GCAGC 3 cut(s) 120, 944, 1557
LweI GCATC 2 cut(s) 445, 1515
MaeI CTAG 1 cut(s) 1298
MaeII ACGT 7 cut(s) 125, 198, 357, 462, 762, 1011, 1116
MaeIII GTNAC 6 cut(s) 83, 326, 485, 820, 974, 1205
MfeI CAATTG 2 cut(s) 642, 957
MflI RGATCY 5 cut(s) 335, 534, 556, 1123, 1216
MhlI GDGCHC 4 cut(s) 243, 841, 852, 922
MluCI AATT 8 cut(s) 479, 571, 627, 642, 776, 875, 957, 1326
MlyI GAGTC 4 cut(s) 615, 688, 1471, 1657
MmeI TCCRAC 6 cut(s) 562, 730, 1078, 1147, 1204, 1631
MslI CAYNNNNRTG 6 cut(s) 513, 827, 829, 831, 1021, 1287
MunI CAATTG 2 cut(s) 642, 957
MvnI CGCG 3 cut(s) 156, 785, 1022
MwoI GCNNNNNNNGC 5 cut(s) 13, 43, 383, 464, 1412
NcoI CCATGG 1 cut(s) 731
NlaIII CATG 7 cut(s) 21, 262, 735, 832, 859, 1020, 1201
NlaIV GGNNCC 5 cut(s) 536, 558, 597, 1125, 1542
NmuCI GTSAC 4 cut(s) 83, 326, 820, 974
NspI RCATGY 2 cut(s) 832, 859
OliI CACNNNNGTG 2 cut(s) 829, 831
PciI ACATGT 2 cut(s) 828, 855
PcsI WCGNNNNNNNCGW 6 cut(s) 306, 363, 398, 663, 1161, 1448
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 7 cut(s) 25, 36, 70, 110, 745, 934, 1572
Ple19I CGATCG 1 cut(s) 1060
PleI GAGTC 4 cut(s) 614, 688, 1471, 1656
PmaCI CACGTG 1 cut(s) 126
PmlI CACGTG 1 cut(s) 126
PpsI GAGTC 4 cut(s) 614, 688, 1471, 1656
Ppu21I YACGTR 1 cut(s) 126
PscI ACATGT 2 cut(s) 828, 855
PsiI TTATAA 1 cut(s) 1331
Psp124BI GAGCTC 2 cut(s) 243, 841
PspCI CACGTG 1 cut(s) 126
PspN4I GGNNCC 5 cut(s) 536, 558, 597, 1125, 1542
PspPI GGNCC 1 cut(s) 663
PsrI GAACNNNNNNTAC 2 cut(s) 192, 224
PsuI RGATCY 5 cut(s) 335, 534, 556, 1123, 1216
PsyI GACNNNGTC 1 cut(s) 200
PvuI CGATCG 1 cut(s) 1060
RsaI GTAC 9 cut(s) 91, 236, 475, 586, 612, 669, 939, 1608, 1636
RsaNI GTAC 9 cut(s) 90, 235, 474, 585, 611, 668, 938, 1607, 1635
RseI CAYNNNNRTG 6 cut(s) 513, 827, 829, 831, 1021, 1287
SacI GAGCTC 2 cut(s) 243, 841
SalI GTCGAC 1 cut(s) 353
SatI GCNGC 7 cut(s) 24, 35, 69, 109, 744, 933, 1571
Sau96I GGNCC 1 cut(s) 663
SchI GAGTC 4 cut(s) 615, 688, 1471, 1657
SduI GDGCHC 4 cut(s) 243, 841, 852, 922
SfaNI GCATC 2 cut(s) 445, 1515
SgrAI CRCCGGYG 1 cut(s) 914
SgrDI CGTCGACG 1 cut(s) 353
SinI GGWCC 1 cut(s) 663
SmiMI CAYNNNNRTG 6 cut(s) 513, 827, 829, 831, 1021, 1287
Sse9I AATT 8 cut(s) 479, 571, 627, 642, 776, 875, 957, 1326
SspMI CTAG 1 cut(s) 1298
SstI GAGCTC 2 cut(s) 243, 841
StyI CCWWGG 1 cut(s) 731
TaiI ACGT 7 cut(s) 128, 201, 360, 465, 765, 1014, 1119
TaqII GACCGA 1 cut(s) 1501
TasI AATT 8 cut(s) 479, 571, 627, 642, 776, 875, 957, 1326
TatI WGTACW 1 cut(s) 1606
TauI GCSGC 4 cut(s) 26, 37, 71, 746
TscAI CASTG 3 cut(s) 335, 620, 1040
TseFI GTSAC 4 cut(s) 83, 326, 820, 974
TseI GCWGC 3 cut(s) 108, 932, 1570
Tsp45I GTSAC 4 cut(s) 83, 326, 820, 974
TspDTI ATGAA 5 cut(s) 26, 415, 1271, 1413, 1613
TspGWI ACGGA 8 cut(s) 134, 314, 355, 797, 993, 1007, 1170, 1505
TspRI CASTG 3 cut(s) 335, 620, 1040
Tth111I GACNNNGTC 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 663
XagI CCTNNNNNAGG 1 cut(s) 1131
XapI RAATTY 4 cut(s) 479, 627, 776, 875
XbaI TCTAGA 1 cut(s) 1297
XceI RCATGY 2 cut(s) 832, 859
XmiI GTMKAC 2 cut(s) 354, 1379
XspI CTAG 1 cut(s) 1298
ZraI GACGTC 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.