Rmu_sc0005347.1_g000004

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005347.1
OG0017051
Physical Location & Seq
Reverse (-)
4082 .. 5497
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005347.1_g000004.1.cds

Sequence Viewer

Length: 867 bp
atgtggcaggttggttggctcagtgatgatggcagcaatggatggctggaagcgggctgcaggggcagtagacttgcggcaaagttgcaggaacgttgcaggggcacaggcgccgggctgcagggcagagggctgggcgcgggagctttctggtattgctcatggaaacctaagtgggactttcttccttcagatgagagatcgaagcttaaagctcggcaaggcgtgcaacatgttgctttggaagaggttgacataaaagattctgtcactatggaaagtgtaccgcctgatggtaagacaatgggtgaaattatgttcaggggaaacactgtgatgagtggttatcttaaagacttgaaagcaacagaggaaaagctcggcaaggcgtgcaacatgttgctttggaagaggggaaacactgtgatgagtggttatcttaaagacttgaaagcaacagaggaagcttttaagggtggatggtttagaagtggggatttagctgtgaaacatcctgataactatagagaagtcaaggaccagttgaaggatgtgattatatctgggggagagaacataagcacagttgaggttgaaactgttttgtttagccatccagcagttcttgaggctgcaattgttgcaaggcctgataatcattggggacaaactccttgtgcatttgtgaaattgaaggacggctttgatgtgaatccgcaagaactaattgagttttgccacaatcatatgcctcattacatggcaccctggaaagtcattttcgatgatataccaaagatttcaacaggaaagatacaaaaattcattctcagagaaaaagcaaaagtcttgggcagcctctcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

288

Amino Acids

32.15

Weight (kDa)

8.13

Isoelectric Point (pI)

34.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 110, 763
AccI GTMKAC 1 cut(s) 70
AccII CGCG 1 cut(s) 140
AciI CCGC 5 cut(s) 53, 77, 140, 287, 716
AclI AACGTT 1 cut(s) 94
AcsI RAATTY 1 cut(s) 821
AcuI CTGAAG 1 cut(s) 174
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 1 cut(s) 285
AfiI CCNNNNNNNGG 2 cut(s) 293, 547
AflIII ACRYGT 2 cut(s) 232, 396
AgsI TTSAA 6 cut(s) 361, 451, 547, 596, 694, 804
AjnI CCWGG 1 cut(s) 767
AluBI AGCT 6 cut(s) 146, 208, 215, 379, 467, 503
AluI AGCT 6 cut(s) 146, 208, 215, 379, 467, 503
AoxI GGCC 1 cut(s) 647
ApeKI GCWGC 5 cut(s) 33, 57, 118, 632, 855
ApoI RAATTY 1 cut(s) 821
AspLEI GCGC 2 cut(s) 113, 140
AspS9I GGNCC 1 cut(s) 538
AsuC2I CCSGG 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 1 cut(s) 538
BaeGI GKGCMC 1 cut(s) 107
BanI GGYRCC 2 cut(s) 110, 763
BbvI GCAGC 4 cut(s) 44, 45, 105, 619
BccI CCATC 5 cut(s) 23, 36, 287, 474, 621
BceAI ACGGC 1 cut(s) 715
BciT130I CCWGG 1 cut(s) 769
BcnI CCSGG 1 cut(s) 115
BfmI CTRYAG 3 cut(s) 58, 119, 523
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 6 cut(s) 34, 58, 78, 119, 633, 856
BlsI GCNGC 6 cut(s) 35, 59, 79, 120, 634, 857
Bme1390I CCNGG 2 cut(s) 115, 769
Bme18I GGWCC 1 cut(s) 538
BmgT120I GGNCC 1 cut(s) 538
BmiI GGNNCC 2 cut(s) 112, 765
BmrFI CCNGG 2 cut(s) 115, 769
BpuEI CTTGAG 1 cut(s) 647
BpuMI CCSGG 1 cut(s) 115
BsaBI GATNNNNATC 1 cut(s) 711
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 1 cut(s) 767
Bsc4I CCNNNNNNNGG 2 cut(s) 293, 547
Bse1I ACTGG 1 cut(s) 541
Bse3DI GCAATG 1 cut(s) 43
Bse8I GATNNNNATC 1 cut(s) 711
BseBI CCWGG 1 cut(s) 769
BseDI CCNNGG 1 cut(s) 767
BseGI GGATG 5 cut(s) 47, 485, 511, 556, 613
BseJI GATNNNNATC 1 cut(s) 711
BseLI CCNNNNNNNGG 2 cut(s) 293, 547
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 34, 844
BseNI ACTGG 1 cut(s) 541
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 4 cut(s) 44, 45, 105, 619
BseYI CCCAGC 1 cut(s) 133
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 1 cut(s) 649
BshNI GGYRCC 2 cut(s) 110, 763
BsiSI CCGG 1 cut(s) 114
BslFI GGGAC 2 cut(s) 191, 678
BslI CCNNNNNNNGG 2 cut(s) 293, 547
BsmFI GGGAC 2 cut(s) 191, 678
BsnI GGCC 1 cut(s) 649
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 1 cut(s) 200
BspACI CCGC 5 cut(s) 53, 77, 140, 287, 716
BspANI GGCC 1 cut(s) 649
BspCNI CTCAG 2 cut(s) 33, 843
BspFNI CGCG 1 cut(s) 140
BspLI GGNNCC 2 cut(s) 112, 765
BspMAI CTGCAG 2 cut(s) 62, 123
BspT107I GGYRCC 2 cut(s) 110, 763
BsrDI GCAATG 1 cut(s) 43
BsrI ACTGG 1 cut(s) 541
BssECI CCNNGG 1 cut(s) 767
BssMI GATC 1 cut(s) 200
BssNI GRCGYC 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 769
Bst4CI ACNGT 4 cut(s) 334, 424, 586, 601
Bst6I CTCTTC 2 cut(s) 240, 404
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 3 cut(s) 226, 390, 641
BstC8I GCNNGC 3 cut(s) 55, 227, 391
BstDEI CTNAG 3 cut(s) 20, 171, 830
BstF5I GGATG 5 cut(s) 47, 485, 511, 556, 613
BstFNI CGCG 1 cut(s) 140
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 2 cut(s) 113, 140
BstKTI GATC 1 cut(s) 203
BstMBI GATC 1 cut(s) 200
BstMWI GCNNNNNNNGC 4 cut(s) 63, 226, 390, 641
BstNI CCWGG 1 cut(s) 769
BstNSI RCATGY 2 cut(s) 236, 400
BstSCI CCNGG 2 cut(s) 113, 767
BstSFI CTRYAG 3 cut(s) 58, 119, 523
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 4 cut(s) 44, 45, 105, 619
BsuRI GGCC 1 cut(s) 649
BtsCI GGATG 5 cut(s) 47, 485, 511, 556, 613
BtsIMutI CAGTG 3 cut(s) 28, 330, 420
Cac8I GCNNGC 3 cut(s) 55, 227, 391
CfoI GCGC 2 cut(s) 113, 140
Cfr13I GGNCC 1 cut(s) 538
Csp6I GTAC 1 cut(s) 284
CviAII CATG 4 cut(s) 162, 233, 397, 760
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 3 cut(s) 20, 171, 830
DinI GGCGCC 1 cut(s) 112
DpnI GATC 1 cut(s) 202
DpnII GATC 1 cut(s) 200
Eam1104I CTCTTC 2 cut(s) 240, 404
EarI CTCTTC 2 cut(s) 240, 404
Eco147I AGGCCT 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 538
Eco57I CTGAAG 1 cut(s) 174
EcoRII CCWGG 1 cut(s) 767
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
FaeI CATG 4 cut(s) 165, 236, 400, 763
FalI AAGNNNNNCTT 4 cut(s) 164, 196, 686, 718
FaqI GGGAC 2 cut(s) 191, 678
FatI CATG 4 cut(s) 161, 232, 396, 759
FauI CCCGC 2 cut(s) 46, 133
FauNDI CATATG 1 cut(s) 747
FblI GTMKAC 1 cut(s) 70
Fnu4HI GCNGC 6 cut(s) 34, 58, 78, 119, 633, 856
FokI GGATG 5 cut(s) 54, 492, 498, 563, 600
Fsp4HI GCNGC 6 cut(s) 34, 58, 78, 119, 633, 856
GlaI GCGC 2 cut(s) 112, 139
GluI GCNGC 6 cut(s) 34, 58, 78, 119, 633, 856
GsaI CCCAGC 1 cut(s) 137
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 1 cut(s) 649
HapII CCGG 1 cut(s) 114
HhaI GCGC 2 cut(s) 113, 140
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 4 cut(s) 165, 236, 400, 763
Hin6I GCGC 2 cut(s) 111, 138
HinP1I GCGC 2 cut(s) 111, 138
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HindIII AAGCTT 2 cut(s) 206, 465
HinfI GANTC 2 cut(s) 263, 712
HpaII CCGG 1 cut(s) 114
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 3 cut(s) 71, 253, 284
Hpy188I TCNGA 2 cut(s) 193, 833
Hpy188III TCNNGA 3 cut(s) 515, 626, 864
Hpy8I GTNNAC 3 cut(s) 71, 253, 284
HpyAV CCTTC 3 cut(s) 198, 541, 688
HpyCH4III ACNGT 4 cut(s) 334, 424, 586, 601
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 9 cut(s) 60, 88, 99, 121, 229, 393, 635, 644, 680
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 226, 390, 641
HpyF3I CTNAG 3 cut(s) 20, 171, 830
HpySE526I ACGT 1 cut(s) 94
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 4 cut(s) 165, 236, 400, 763
HspAI GCGC 2 cut(s) 111, 138
KasI GGCGCC 1 cut(s) 110
Kzo9I GATC 1 cut(s) 200
LmnI GCTCC 1 cut(s) 143
Lsp1109I GCAGC 4 cut(s) 44, 45, 105, 619
MaeII ACGT 1 cut(s) 94
MaeIII GTNAC 1 cut(s) 268
MalI GATC 1 cut(s) 202
MboI GATC 1 cut(s) 200
MboII GAAGA 3 cut(s) 176, 257, 421
MfeI CAATTG 1 cut(s) 636
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 5 cut(s) 312, 636, 689, 726, 821
Mly113I GGCGCC 1 cut(s) 111
MnlI CCTC 8 cut(s) 122, 241, 364, 405, 454, 583, 622, 762
MseI TTAA 4 cut(s) 210, 351, 441, 471
MslI CAYNNNNRTG 2 cut(s) 335, 425
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 2 cut(s) 115, 769
MunI CAATTG 1 cut(s) 636
MvaI CCWGG 1 cut(s) 769
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 4 cut(s) 63, 226, 390, 641
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 1 cut(s) 115
NdeI CATATG 1 cut(s) 747
NdeII GATC 1 cut(s) 200
NlaIII CATG 4 cut(s) 165, 236, 400, 763
NlaIV GGNNCC 2 cut(s) 112, 765
NmeAIII GCCGAG 2 cut(s) 196, 360
NmuCI GTSAC 1 cut(s) 268
NspI RCATGY 2 cut(s) 236, 400
PceI AGGCCT 1 cut(s) 649
PciI ACATGT 2 cut(s) 232, 396
PfeI GAWTC 2 cut(s) 263, 712
PkrI GCNGC 6 cut(s) 35, 59, 79, 120, 634, 857
PluTI GGCGCC 1 cut(s) 114
PscI ACATGT 2 cut(s) 232, 396
Psp1406I AACGTT 1 cut(s) 94
Psp6I CCWGG 1 cut(s) 767
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 1 cut(s) 767
PspN4I GGNNCC 2 cut(s) 112, 765
PspPI GGNCC 1 cut(s) 538
PstI CTGCAG 2 cut(s) 62, 123
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
RseI CAYNNNNRTG 2 cut(s) 335, 425
SaqAI TTAA 4 cut(s) 210, 351, 441, 471
SatI GCNGC 6 cut(s) 34, 58, 78, 119, 633, 856
Sau3AI GATC 1 cut(s) 200
Sau96I GGNCC 1 cut(s) 538
ScrFI CCNGG 2 cut(s) 115, 769
SduI GDGCHC 1 cut(s) 107
SfcI CTRYAG 3 cut(s) 58, 119, 523
SfoI GGCGCC 1 cut(s) 112
SinI GGWCC 1 cut(s) 538
SmiMI CAYNNNNRTG 2 cut(s) 335, 425
SmlI CTYRAG 1 cut(s) 626
SmoI CTYRAG 1 cut(s) 626
Sse9I AATT 5 cut(s) 312, 636, 689, 726, 821
SseBI AGGCCT 1 cut(s) 649
SsiI CCGC 5 cut(s) 53, 77, 140, 287, 716
SspDI GGCGCC 1 cut(s) 110
StuI AGGCCT 1 cut(s) 649
StyD4I CCNGG 2 cut(s) 113, 767
TaaI ACNGT 4 cut(s) 334, 424, 586, 601
TaiI ACGT 1 cut(s) 97
TaqI TCGA 2 cut(s) 203, 783
TasI AATT 5 cut(s) 312, 636, 689, 726, 821
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 2 cut(s) 263, 712
Tru1I TTAA 4 cut(s) 210, 351, 441, 471
Tru9I TTAA 4 cut(s) 210, 351, 441, 471
TscAI CASTG 3 cut(s) 28, 337, 427
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 5 cut(s) 33, 57, 118, 632, 855
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 1 cut(s) 814
TspRI CASTG 3 cut(s) 28, 337, 427
VpaK11BI GGWCC 1 cut(s) 538
XapI RAATTY 1 cut(s) 821
XceI RCATGY 2 cut(s) 236, 400
XmiI GTMKAC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.