AT5G35220

zinc metalloprotease EGY1, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011472
Physical Location & Seq
Reverse (-)
13484403 .. 13487594
3192 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35220.1

Sequence Viewer

Length: 1647 bp
ATGGGGACTCTCACCAGCGTCGCTTTTGCCGCTGCCGTTAACATTAGATTCCGATCATTTCACCGGGAAAACATTAAGACAACAATTACAACTCTGCCCAAATGGCAAAAGAGACTCTGTTTCTCTTCTACAGAGGATTCTCATAGATTTAGGATTGCAAAGTGTTTAGGCAATGACGAAAACAGTAACAGAGACGACAGTATCGGAGAAAACGGAGAAACCCACAAGTCTTCGGTTGTTAAAACGGCAACGTTTGAAGAAGAAGATGAAGAGACAAGTAAGAGTAGCTCTACTACTAGCAGCAGCAACGAGTTCGGTTCCGACAAGACTTCAATGCCATCAACTATTGATCCAACATATAGTAGCTTCCAAATAGATTCGTTTAAGCTAATGGAGCTTCTTGGACCAGAGAAAGTAGATCCTGCAGATGTCAAGTTAATAAAGGACAAACTTTTCGGTTATTCGACATTTTGGGTGACGAAAGAAGAGCCATTTGGGGACCTTGGGGAAGGAATTCTCTTTCTTGGGAACTTGAGAGGAAAGAAAGAAGATGTTTTTGCAAAACTTCAGAGAAAACTAGTTGAAGTTGCTAGTGATAAATATAATTTATTCATGATTGAGGAGCCTAACTCAGAAGGACCTGATCCTCGCGGTGGTGCTCGTGTTAGCTTTGGTTTGCTTCGTAAAGAAGTTTCGGAGCCTGGACCAACTACACTCTGGCAGTATGTGATTGCTTTAATATTGTTTCTTTTGACTATTGGTTCCTCTGTGGAGTTAGGAATTGCTTCTCAGATTAACCGCTTACCTCCTGAGGTGGTAAAGTATTTCACCGATCCAAATGCTGTTGAACCACCTGATATGGAGCTGCTGTATCCATTTGTAGATGCTGCATTGCCTTTAGCTTATGGTGTATTGGGAATTCTTTTGTTTCATGAATTAGGGCACTTTCTTGCTGCAGTTCCAAAGAAAGTTAAGCTTAGCATTCCATACTTCATTCCAAACATTACACTCGGCAGCTTTGGGGCAATCACACAGTTTAAGTCGATTCTTCCTGACCGGAGTACAAAAGTTGACATTTCGCTAGCTGGTCCATTTGCTGGAGCTGCACTCTCAGTTTCCATGTTTGCTGTTGGTCTGTTCCTATCTACAGAACCAGATGCAGCTAACGACCTGGTCCAGGTTCCCAGCATGTTATTTCAAGGTTCATTACTTCTTGGACTCATCAGCAGAGCAACTCTGGGATATGCAGCTTTGCATGCTGCAACAGTTTCAATCCACCCACTTGTAATTGCTGGATGGTGTGGTTTAACAACAACAGCATTTAATATGCTTCCAGTGGGATGTTTGGATGGAGGAAGAGCTGTACAGGGAGCATTCGGGAAAAATGCATTGGTTACATTTGGTTTGTCAACCTATGTAATGCTTGGACTCAGAGTGCTCGGTGGCCCTTTGGCACTTCCTTGGGGACTCTACGTATTAATCTGCCAGAGAACACCTGAAAAACCGTGTCTAAACGACGTGACCGAGGTTGGAACGTGGAGGAAGGCCCTTGTAGGGATTGCACTAATCCTGGTGGTTTTGACACTCCTGCCTGTATGGGACGAACTCGCAGAAGAAGTAGGCATAGGGCTTGTAACCACATTTTGA

Protein Analysis

548

Amino Acids

59.5

Weight (kDa)

5.32

Isoelectric Point (pI)

32.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M50 PF02163 301 - 460 7.5e-20 Peptidase family M50
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1097
AccII CGCG 1 cut(s) 651
AciI CCGC 3 cut(s) 30, 651, 799
AclI AACGTT 1 cut(s) 251
AclWI GGATC 4 cut(s) 344, 413, 638, 827
AcsI RAATTY 2 cut(s) 513, 918
AcuI CTGAAG 1 cut(s) 551
AfaI GTAC 2 cut(s) 1063, 1365
AfiI CCNNNNNNNGG 4 cut(s) 653, 1097, 1177, 1554
AgsI TTSAA 6 cut(s) 257, 333, 584, 848, 1199, 1272
AhlI ACTAGT 1 cut(s) 577
AjiI CACGTC 1 cut(s) 1519
AjnI CCWGG 4 cut(s) 700, 1170, 1176, 1569
AjuI GAANNNNNNNTTGG 4 cut(s) 477, 509, 1373, 1405
Alw21I GWGCWC 2 cut(s) 661, 1440
Alw26I GTCTC 3 cut(s) 106, 186, 266
AlwI GGATC 4 cut(s) 344, 413, 638, 827
AoxI GGCC 2 cut(s) 1444, 1545
ApoI RAATTY 2 cut(s) 513, 918
ArsI GACNNNNNNTTYG 2 cut(s) 437, 469
AseI ATTAAT 1 cut(s) 1478
Asp700I GAANNNNTTC 2 cut(s) 513, 784
AspS9I GGNCC 8 cut(s) 404, 499, 638, 704, 1088, 1174, 1445, 1546
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 4 cut(s) 4, 53, 487, 820
AsuNHI GCTAGC 1 cut(s) 1081
AvaII GGWCC 6 cut(s) 404, 499, 638, 704, 1088, 1174
AxyI CCTNAGG 1 cut(s) 810
BaeGI GKGCMC 1 cut(s) 945
BaeI ACNNNNGTAYC 2 cut(s) 184, 217
BauI CACGAG 1 cut(s) 660
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 2 cut(s) 661, 1440
BccI CCATC 3 cut(s) 346, 1290, 1343
BceAI ACGGC 2 cut(s) 20, 261
BcgI CGANNNNNNTGC 4 cut(s) 295, 329, 821, 855
BciT130I CCWGG 4 cut(s) 702, 1172, 1178, 1571
BciVI GTATCC 1 cut(s) 882
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 3 cut(s) 106, 186, 266
BcuI ACTAGT 1 cut(s) 577
BfaI CTAG 4 cut(s) 297, 578, 591, 1082
BfmI CTRYAG 4 cut(s) 129, 423, 954, 1146
BfuI GTATCC 1 cut(s) 882
BglI GCCNNNNNGGC 1 cut(s) 103
BlpI GCTNAGC 1 cut(s) 977
Bme1390I CCNGG 5 cut(s) 65, 702, 1172, 1178, 1571
Bme18I GGWCC 6 cut(s) 404, 499, 638, 704, 1088, 1174
BmgBI CACGTC 1 cut(s) 1519
BmgT120I GGNCC 8 cut(s) 404, 499, 638, 704, 1088, 1174, 1445, 1546
BmiI GGNNCC 6 cut(s) 319, 500, 624, 699, 763, 1182
BmrFI CCNGG 5 cut(s) 65, 702, 1172, 1178, 1571
BmsI GCATC 2 cut(s) 874, 1147
BmtI GCTAGC 1 cut(s) 1085
BpiI GAAGAC 1 cut(s) 222
BplI GAGNNNNNCTC 4 cut(s) 614, 646, 1092, 1124
BpmI CTGGAG 1 cut(s) 1119
Bpu1102I GCTNAGC 1 cut(s) 977
BpuEI CTTGAG 1 cut(s) 553
BpuMI CCSGG 1 cut(s) 65
BsaAI YACGTR 1 cut(s) 1474
BsaBI GATNNNNATC 1 cut(s) 52
BsaJI CCNNGG 3 cut(s) 502, 1460, 1524
BsaWI WCCGGW 1 cut(s) 1056
BsaXI ACNNNNNCTCC 2 cut(s) 854, 884
Bsc4I CCNNNNNNNGG 4 cut(s) 653, 1097, 1177, 1554
Bse1I ACTGG 1 cut(s) 1334
Bse21I CCTNAGG 1 cut(s) 810
Bse3DI GCAATG 2 cut(s) 178, 890
Bse8I GATNNNNATC 1 cut(s) 52
BseBI CCWGG 4 cut(s) 702, 1172, 1178, 1571
BseDI CCNNGG 3 cut(s) 502, 1460, 1524
BseGI GGATG 3 cut(s) 1301, 1346, 1354
BseJI GATNNNNATC 1 cut(s) 52
BseLI CCNNNNNNNGG 4 cut(s) 653, 1097, 1177, 1554
BseMI GCAATG 2 cut(s) 178, 890
BseMII CTCAG 5 cut(s) 645, 801, 803, 1125, 1444
BseNI ACTGG 1 cut(s) 1334
BseRI GAGGAG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 945
BseYI CCCAGC 1 cut(s) 1184
BsgI GTGCAG 1 cut(s) 1089
Bsh1236I CGCG 1 cut(s) 651
BshFI GGCC 2 cut(s) 1446, 1547
BsiHKAI GWGCWC 2 cut(s) 661, 1440
BsiSI CCGG 2 cut(s) 64, 1057
BslFI GGGAC 4 cut(s) 19, 512, 1479, 1613
BslI CCNNNNNNNGG 4 cut(s) 653, 1097, 1177, 1554
BsmAI GTCTC 3 cut(s) 106, 186, 266
BsmBI CGTCTC 1 cut(s) 186
BsmFI GGGAC 4 cut(s) 19, 512, 1479, 1613
BsmI GAATGC 2 cut(s) 981, 1373
BsnI GGCC 2 cut(s) 1446, 1547
Bsp1286I GDGCHC 3 cut(s) 661, 945, 1440
Bsp1407I TGTACA 1 cut(s) 1363
Bsp143I GATC 5 cut(s) 53, 349, 418, 643, 832
Bsp1720I GCTNAGC 1 cut(s) 977
BspACI CCGC 3 cut(s) 30, 651, 799
BspANI GGCC 2 cut(s) 1446, 1547
BspCNI CTCAG 5 cut(s) 644, 802, 802, 1124, 1443
BspFNI CGCG 1 cut(s) 651
BspHI TCATGA 2 cut(s) 612, 931
BspLI GGNNCC 6 cut(s) 319, 500, 624, 699, 763, 1182
BspMAI CTGCAG 2 cut(s) 427, 958
BspOI GCTAGC 1 cut(s) 1085
BspPI GGATC 4 cut(s) 344, 413, 638, 827
BspQI GCTCTTC 2 cut(s) 480, 1351
BsrDI GCAATG 2 cut(s) 178, 890
BsrGI TGTACA 1 cut(s) 1363
BsrI ACTGG 1 cut(s) 1334
BssECI CCNNGG 3 cut(s) 502, 1460, 1524
BssMI GATC 5 cut(s) 53, 349, 418, 643, 832
BssSI CACGAG 1 cut(s) 660
BssT1I CCWWGG 2 cut(s) 502, 1460
Bst2BI CACGAG 1 cut(s) 660
Bst2UI CCWGG 4 cut(s) 702, 1172, 1178, 1571
Bst4CI ACNGT 5 cut(s) 185, 200, 1035, 1267, 1506
Bst6I CTCTTC 4 cut(s) 130, 264, 480, 1351
BstAUI TGTACA 1 cut(s) 1363
BstBAI YACGTR 1 cut(s) 1474
BstC8I GCNNGC 2 cut(s) 1083, 1257
BstDEI CTNAG 6 cut(s) 631, 789, 810, 977, 1111, 1430
BstENI CCTNNNNNAGG 1 cut(s) 1175
BstF5I GGATG 3 cut(s) 1301, 1346, 1354
BstFNI CGCG 1 cut(s) 651
BstKTI GATC 5 cut(s) 56, 352, 421, 646, 835
BstMAI GTCTC 3 cut(s) 106, 186, 266
BstMBI GATC 5 cut(s) 53, 349, 418, 643, 832
BstMWI GCNNNNNNNGC 5 cut(s) 29, 103, 394, 1103, 1256
BstNI CCWGG 4 cut(s) 702, 1172, 1178, 1571
BstNSI RCATGY 2 cut(s) 1192, 1259
BstSCI CCNGG 5 cut(s) 63, 700, 1170, 1176, 1569
BstSFI CTRYAG 4 cut(s) 129, 423, 954, 1146
BstSLI GKGCMC 1 cut(s) 945
BstSNI TACGTA 1 cut(s) 1474
BstUI CGCG 1 cut(s) 651
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
Bsu36I CCTNAGG 1 cut(s) 810
BsuI GTATCC 1 cut(s) 882
BsuRI GGCC 2 cut(s) 1446, 1547
BtrI CACGTC 1 cut(s) 1519
BtsCI GGATG 3 cut(s) 1301, 1346, 1354
BtsIMutI CAGTG 1 cut(s) 1341
Cac8I GCNNGC 2 cut(s) 1083, 1257
CciI TCATGA 2 cut(s) 612, 931
Cfr13I GGNCC 8 cut(s) 404, 499, 638, 704, 1088, 1174, 1445, 1546
CseI GACGC 1 cut(s) 7
CsiI ACCWGGT 1 cut(s) 1170
Csp6I GTAC 2 cut(s) 1062, 1364
CviAII CATG 5 cut(s) 613, 932, 1120, 1189, 1256
CviQI GTAC 2 cut(s) 1062, 1364
DdeI CTNAG 6 cut(s) 631, 789, 810, 977, 1111, 1430
DpnI GATC 5 cut(s) 55, 351, 420, 645, 834
DpnII GATC 5 cut(s) 53, 349, 418, 643, 832
Eam1104I CTCTTC 4 cut(s) 130, 264, 480, 1351
EarI CTCTTC 4 cut(s) 130, 264, 480, 1351
Eco105I TACGTA 1 cut(s) 1474
Eco130I CCWWGG 2 cut(s) 502, 1460
Eco47I GGWCC 6 cut(s) 404, 499, 638, 704, 1088, 1174
Eco57I CTGAAG 1 cut(s) 551
Eco81I CCTNAGG 1 cut(s) 810
EcoNI CCTNNNNNAGG 1 cut(s) 1175
EcoO109I RGGNCCY 3 cut(s) 499, 638, 1546
EcoRI GAATTC 2 cut(s) 513, 918
EcoRII CCWGG 4 cut(s) 700, 1170, 1176, 1569
EcoT14I CCWWGG 2 cut(s) 502, 1460
EcoT22I ATGCAT 1 cut(s) 1390
ErhI CCWWGG 2 cut(s) 502, 1460
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 5 cut(s) 616, 935, 1123, 1192, 1259
FalI AAGNNNNNCTT 2 cut(s) 960, 992
FaqI GGGAC 4 cut(s) 19, 512, 1479, 1613
FatI CATG 5 cut(s) 612, 931, 1119, 1188, 1255
FokI GGATG 3 cut(s) 1308, 1353, 1361
FspBI CTAG 4 cut(s) 297, 578, 591, 1082
GsaI CCCAGC 1 cut(s) 1188
GsuI CTGGAG 1 cut(s) 1119
HaeIII GGCC 2 cut(s) 1446, 1547
HapII CCGG 2 cut(s) 64, 1057
HgaI GACGC 1 cut(s) 7
Hin1II CATG 5 cut(s) 616, 935, 1123, 1192, 1259
HincII GTYRAC 3 cut(s) 40, 1072, 1410
HindII GTYRAC 3 cut(s) 40, 1072, 1410
HindIII AAGCTT 1 cut(s) 974
HinfI GANTC 9 cut(s) 7, 48, 114, 137, 377, 1045, 1218, 1428, 1467
HpaI GTTAAC 1 cut(s) 40
HpaII CCGG 2 cut(s) 64, 1057
HphI GGTGA 4 cut(s) 4, 53, 487, 820
Hpy166II GTNNAC 3 cut(s) 40, 1072, 1410
Hpy188I TCNGA 8 cut(s) 53, 206, 322, 570, 634, 697, 792, 1433
Hpy188III TCNNGA 5 cut(s) 613, 809, 932, 1052, 1378
Hpy8I GTNNAC 3 cut(s) 40, 1072, 1410
Hpy99I CGWCG 2 cut(s) 23, 1520
HpyAV CCTTC 3 cut(s) 503, 629, 1537
HpyCH4III ACNGT 5 cut(s) 185, 200, 1035, 1267, 1506
HpyCH4IV ACGT 4 cut(s) 251, 1473, 1518, 1535
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 103, 394, 1103, 1256
HpyF3I CTNAG 6 cut(s) 631, 789, 810, 977, 1111, 1430
HpySE526I ACGT 4 cut(s) 251, 1473, 1518, 1535
Hsp92II CATG 5 cut(s) 616, 935, 1123, 1192, 1259
KspAI GTTAAC 1 cut(s) 40
Kzo9I GATC 5 cut(s) 53, 349, 418, 643, 832
LguI GCTCTTC 2 cut(s) 480, 1351
LmnI GCTCC 6 cut(s) 394, 622, 697, 862, 1100, 1370
LweI GCATC 2 cut(s) 874, 1147
MabI ACCWGGT 1 cut(s) 1170
MaeI CTAG 4 cut(s) 297, 578, 591, 1082
MaeII ACGT 4 cut(s) 251, 1473, 1518, 1535
MaeIII GTNAC 5 cut(s) 185, 475, 1393, 1519, 1633
MalI GATC 5 cut(s) 55, 351, 420, 645, 834
MboI GATC 5 cut(s) 53, 349, 418, 643, 832
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 3 cut(s) 661, 945, 1440
MluCI AATT 7 cut(s) 84, 513, 604, 780, 918, 935, 1287
MlyI GAGTC 4 cut(s) 108, 1212, 1422, 1461
MmeI TCCRAC 3 cut(s) 345, 377, 1510
Mph1103I ATGCAT 1 cut(s) 1390
MroXI GAANNNNTTC 2 cut(s) 513, 784
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 2 cut(s) 64, 1057
MspR9I CCNGG 5 cut(s) 65, 702, 1172, 1178, 1571
Mva1269I GAATGC 2 cut(s) 981, 1373
MvaI CCWGG 4 cut(s) 702, 1172, 1178, 1571
MvnI CGCG 1 cut(s) 651
MwoI GCNNNNNNNGC 5 cut(s) 29, 103, 394, 1103, 1256
NciI CCSGG 1 cut(s) 65
NdeII GATC 5 cut(s) 53, 349, 418, 643, 832
NheI GCTAGC 1 cut(s) 1081
NlaIII CATG 5 cut(s) 616, 935, 1123, 1192, 1259
NlaIV GGNNCC 6 cut(s) 319, 500, 624, 699, 763, 1182
NmeAIII GCCGAG 1 cut(s) 990
NmuCI GTSAC 2 cut(s) 475, 1519
NsiI ATGCAT 1 cut(s) 1390
NspI RCATGY 2 cut(s) 1192, 1259
PaeI GCATGC 1 cut(s) 1259
PagI TCATGA 2 cut(s) 612, 931
PciSI GCTCTTC 2 cut(s) 480, 1351
PcsI WCGNNNNNNNCGW 1 cut(s) 1521
PctI GAATGC 2 cut(s) 981, 1373
PdmI GAANNNNTTC 2 cut(s) 513, 784
PfeI GAWTC 4 cut(s) 48, 137, 377, 1045
PflFI GACNNNGTC 1 cut(s) 1172
PflMI CCANNNNNTGG 1 cut(s) 1097
PleI GAGTC 4 cut(s) 108, 1212, 1422, 1461
PpsI GAGTC 4 cut(s) 108, 1212, 1422, 1461
Ppu21I YACGTR 1 cut(s) 1474
PpuMI RGGWCCY 2 cut(s) 499, 638
PshBI ATTAAT 1 cut(s) 1478
Psp1406I AACGTT 1 cut(s) 251
Psp5II RGGWCCY 2 cut(s) 499, 638
Psp6I CCWGG 4 cut(s) 700, 1170, 1176, 1569
PspFI CCCAGC 1 cut(s) 1184
PspGI CCWGG 4 cut(s) 700, 1170, 1176, 1569
PspN4I GGNNCC 6 cut(s) 319, 500, 624, 699, 763, 1182
PspPI GGNCC 8 cut(s) 404, 499, 638, 704, 1088, 1174, 1445, 1546
PspPPI RGGWCCY 2 cut(s) 499, 638
PstI CTGCAG 2 cut(s) 427, 958
PsuI RGATCY 1 cut(s) 418
PsyI GACNNNGTC 1 cut(s) 1172
RsaI GTAC 2 cut(s) 1063, 1365
RsaNI GTAC 2 cut(s) 1062, 1364
SapI GCTCTTC 2 cut(s) 480, 1351
Sau3AI GATC 5 cut(s) 53, 349, 418, 643, 832
Sau96I GGNCC 8 cut(s) 404, 499, 638, 704, 1088, 1174, 1445, 1546
SchI GAGTC 4 cut(s) 108, 1212, 1422, 1461
ScrFI CCNGG 5 cut(s) 65, 702, 1172, 1178, 1571
SduI GDGCHC 3 cut(s) 661, 945, 1440
SexAI ACCWGGT 1 cut(s) 1170
SfaNI GCATC 2 cut(s) 874, 1147
SfcI CTRYAG 4 cut(s) 129, 423, 954, 1146
SinI GGWCC 6 cut(s) 404, 499, 638, 704, 1088, 1174
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
SnaBI TACGTA 1 cut(s) 1474
SpeI ACTAGT 1 cut(s) 577
SphI GCATGC 1 cut(s) 1259
Sse9I AATT 7 cut(s) 84, 513, 604, 780, 918, 935, 1287
SsiI CCGC 3 cut(s) 30, 651, 799
SspI AATATT 1 cut(s) 741
SspMI CTAG 4 cut(s) 297, 578, 591, 1082
StyD4I CCNGG 5 cut(s) 63, 700, 1170, 1176, 1569
StyI CCWWGG 2 cut(s) 502, 1460
TaaI ACNGT 5 cut(s) 185, 200, 1035, 1267, 1506
TaiI ACGT 4 cut(s) 254, 1476, 1521, 1538
TaqI TCGA 2 cut(s) 464, 1043
TaqII GACCGA 1 cut(s) 1538
TasI AATT 7 cut(s) 84, 513, 604, 780, 918, 935, 1287
TatI WGTACW 2 cut(s) 1061, 1363
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 4 cut(s) 48, 137, 377, 1045
TscAI CASTG 1 cut(s) 1341
TseFI GTSAC 2 cut(s) 475, 1519
Tsp45I GTSAC 2 cut(s) 475, 1519
TspDTI ATGAA 6 cut(s) 282, 601, 920, 948, 982, 1194
TspGWI ACGGA 1 cut(s) 228
TspRI CASTG 1 cut(s) 1341
Tth111I GACNNNGTC 1 cut(s) 1172
Van91I CCANNNNNTGG 1 cut(s) 1097
VpaK11BI GGWCC 6 cut(s) 404, 499, 638, 704, 1088, 1174
VspI ATTAAT 1 cut(s) 1478
XagI CCTNNNNNAGG 1 cut(s) 1175
XapI RAATTY 2 cut(s) 513, 918
XceI RCATGY 2 cut(s) 1192, 1259
XcmI CCANNNNNNNNNTGG 1 cut(s) 714
XmnI GAANNNNTTC 2 cut(s) 513, 784
XspI CTAG 4 cut(s) 297, 578, 591, 1082
Zsp2I ATGCAT 1 cut(s) 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.