Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0011472

Physical Location & Seq

Genomic Coordinates Reverse (-)

746229 .. 750916

Gene Structure 4688 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1007000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1698 bp

ATGGGAACGCTCACCGGCTGCACTTTCAGCACCCTCGATTTCAACCTCACCCTCAAAACCCGCCGCCAAATTCCTAGACTGCCATCGCCCAAGAAACCCAGGAGGAAGAATTCTTCATGCTTCTTGTGCGGCAGAGCGAGTGTAAATACCCAGAGAATTTGTTGGGTTCCGCGTAGGAATTTTGATAGGCTTAGATGTTATAGTTCTAGCGATAGTGGTGACGGTGACGGAGAGAGTAGCAGCAGCAGCAGCGGGAGTAGTGGTAATAAGTCAACCGACTCCACGACGGCGACGAAGGCGGTGACAACGACGGAAGTAGACGAAGATAAAACGCGCGCAAATGACTATGACTCTGAACCTCCATCGGTTTCTTCCAGGCCGCCAACTATATCACCACTTGGACCTGCGTACAATAATTTCCAGGTTGACTCTTTTAAACTGATGGAACTCCTTGGACCCGAGAAGGTTGATCCTGCTGATGTAAAGCTGATTAAGAAAGCACTTTTTGGTTATTCTACCTTTTGGGTGACCAAAGAGGAGCCTTTTGGAGACCTCGGTGAGGGCATTCTTTTCCTTGGGAATTTGAGGGGAAAGAGAGAAGAGGTATTTGCCAAATTCCAACGTCTACTGGCTGAAGTCACGGGTAACAAGTACAACCTTTTTATGGTGGAGGAACCCAATGCAGAAGATCTAGATCCACGTGGTGGTCCACGTGTTAGCTTTGGCTTGCTGCGGAAAGAGGTCTCAGAACCAGGGCCAACCACACTTTGGCAATATGTAATTGCTTTCTTGTTATTCCTTCTAACAATTGGATCATCTGTGGAGTTAGGAATTGCATCTCAGATAAATAAACTTCCGCCAGAGGTGGTGAAATACTTCACAGACCCAAATGCCGTTGACCCACCAGATATGGAGTTGCTATCCCCGTATGTAGATTCCGCTTTGCCCTTAGCATATGGTGTTCTGGGGGTTTTGCTGTTTCATGAAGTTGGGCATTTTTTGGCTGCTTTCCCAAACAAAGTAAAACTTAGCATTCCTTTCTTCATTCCTAATATTACTCTTGGTAGCTTTGGCGCCATCACTCAGTTTAAATCCATTCTTCCTGATCGGAGCACAAAAGTCGACATATCACTGGCGGGTCCTTTTGCTGGTGCTGCGTTGTCATTGTCAATGTTTGCTGTTGGCCTGCTGCTCTCATCTAATCCCAATGCCACAGGAGAGTTGGTGCAGGTTCCGAGCACGCTGTTTCAAGGTTCTTTGCTTCTTGGACTCATCAGTAGAGCCACTCTTGGTTATGCATCAATGCATGCTGCGACTGTTCCAATTCATCCTCTTGTGATTGCTGGCTGGTGTGGGTTGACAACAACAGCTTTTAATATGCTTCCAGTGGGCTGTCTTGACGGTGGAAGAGCTGTGCAGGGCGCTTTTGGAAAAAATGCACTAGTTGCCTTTGGCTTGACGACATACACCATGCTTGGATTGGGAGTGCTTGGCGGACCTCTGTCACTTCCATGGGGACTCTATGTGCTCATATGCCAGAGGACTCCCGAGAAACCGTGTCTGAATGATGTAACAGAGGTTGGAACGTGGAGGAAAACAATTGTTACAGCGGCCGTGTTCCTTGTTGTACTCACACTTCTTCCTGTATGGGATGAGCTTGCCGAGGAGCTGGGTATAGGTCTTGTAAGCACATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009651 GO:0009657 GO:0009658 GO:0009668 GO:0009719 GO:0009723 GO:0009725 GO:0009959 GO:0009987 GO:0010027 GO:0010033 GO:0010207 GO:0015979 GO:0016043 GO:0016787 GO:0019538 GO:0019684 GO:0022607 GO:0034622 GO:0042221 GO:0043157 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048564 GO:0050896 GO:0060359 GO:0061024 GO:0065003 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M50

Protein Analysis

566

Amino Acids

60.94

Weight (kDa)

6.2

Isoelectric Point (pI)

36.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M50	PF02163	318 - 477	8.2e-19	Peptidase family M50

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011472)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35220
fragaria_vesca	FvH4_1g28520 FvH4_1g28520
malus_domestica	MD12G1009600.v1.1 MD14G1007000.v1.1
prunus_persica	Prupe.7G011600_v2.0.a1 Prupe.7G011600_v2.0.a1
pyrus_communis	pycom12g00740 pycom14g00550
rosa_chinensis	RchiOBHm_Chr3g0496231
rosa_laevigata	RLG00000022545
rosa_multiflora	Rmu_sc0004080.1_g000013 Rmu_ssc0000454.1_g000006
rosa_roxburghii	Rroxscaffold_6G00389320
rosa_rugosa	Rorug03G0280800 Rorug03G0280900
rosa_samantha	Rh3BG364000 Rh3CG360900 Rh3DG363900
rosa_wichuraiana	Rw3G028710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	412, 1219
AccB1I	GGYRCC	1 cut(s)	1073
AccB7I	CCANNNNNTGG	2 cut(s)	704, 768
AccI	GTMKAC	3 cut(s)	318, 625, 1122
AccII	CGCG	3 cut(s)	172, 334, 336
AclWI	GGATC	3 cut(s)	464, 689, 820
AcoI	YGGCCR	1 cut(s)	1611
AcsI	RAATTY	6 cut(s)	69, 109, 156, 178, 580, 614
AcuI	CTGAAG	1 cut(s)	654
AcvI	CACGTG	2 cut(s)	701, 713
AcyI	GRCGYC	1 cut(s)	1074
AdeI	CACNNNGTG	1 cut(s)	704
AfaI	GTAC	3 cut(s)	410, 653, 1629
AfiI	CCNNNNNNNGG	5 cut(s)	559, 664, 704, 768, 1148
AflIII	ACRYGT	1 cut(s)	712
AgsI	TTSAA	2 cut(s)	43, 1250
AhlI	ACTAGT	1 cut(s)	1441
AjnI	CCWGG	4 cut(s)	98, 374, 420, 751
AluBI	AGCT	7 cut(s)	487, 720, 1068, 1370, 1412, 1657, 1669
AluI	AGCT	7 cut(s)	487, 720, 1068, 1370, 1412, 1657, 1669
Alw21I	GWGCWC	3 cut(s)	1115, 1241, 1530
Alw26I	GTCTC	2 cut(s)	543, 748
AlwI	GGATC	3 cut(s)	464, 689, 820
Ama87I	CYCGRG	2 cut(s)	458, 1547
AoxI	GGCC	4 cut(s)	377, 755, 1183, 1611
ApoI	RAATTY	6 cut(s)	69, 109, 156, 178, 580, 614
Asp700I	GAANNNNTTC	1 cut(s)	875
AspLEI	GCGC	4 cut(s)	336, 338, 1076, 1424
AspS9I	GGNCC	6 cut(s)	401, 455, 707, 755, 1139, 1496
AsuHPI	GGTGA	9 cut(s)	4, 40, 230, 236, 313, 384, 538, 569, 880
AvaI	CYCGRG	2 cut(s)	458, 1547
AvaII	GGWCC	5 cut(s)	401, 455, 707, 1139, 1496
BanI	GGYRCC	1 cut(s)	1073
BarI	GAAGNNNNNNTAC	2 cut(s)	1620, 1652
BbrPI	CACGTG	2 cut(s)	701, 713
Bbv12I	GWGCWC	3 cut(s)	1115, 1241, 1530
BccI	CCATC	4 cut(s)	91, 370, 436, 1085
BceAI	ACGGC	3 cut(s)	303, 878, 1598
BcgI	CGANNNNNNTGC	2 cut(s)	1102, 1136
BciT130I	CCWGG	4 cut(s)	100, 376, 422, 753
BcoDI	GTCTC	2 cut(s)	543, 748
BcuI	ACTAGT	1 cut(s)	1441
BfaI	CTAG	4 cut(s)	75, 207, 692, 1442
BfoI	RGCGCY	2 cut(s)	1077, 1425
BfuAI	ACCTGC	2 cut(s)	412, 1219
BglII	AGATCT	1 cut(s)	688
Bme1390I	CCNGG	4 cut(s)	100, 376, 422, 753
Bme18I	GGWCC	5 cut(s)	401, 455, 707, 1139, 1496
BmeT110I	CYCGRG	2 cut(s)	458, 1547
BmgT120I	GGNCC	6 cut(s)	401, 455, 707, 755, 1139, 1496
BmiI	GGNNCC	7 cut(s)	168, 457, 540, 675, 1075, 1140, 1233
BmrFI	CCNGG	4 cut(s)	100, 376, 422, 753
BmsI	GCATC	2 cut(s)	845, 1307
BoxI	GACNNNNGTC	1 cut(s)	1501
Bpu10I	CCTNAGC	1 cut(s)	949
BsaAI	YACGTR	2 cut(s)	701, 713
BsaBI	GATNNNNATC	1 cut(s)	693
BsaHI	GRCGYC	1 cut(s)	1074
BsaI	GGTCTC	2 cut(s)	543, 748
BsaJI	CCNNGG	7 cut(s)	98, 451, 553, 574, 752, 1511, 1662
BsaXI	ACNNNNNCTCC	4 cut(s)	247, 277, 1209, 1239
Bsc4I	CCNNNNNNNGG	5 cut(s)	559, 664, 704, 768, 1148
Bse118I	RCCGGY	1 cut(s)	14
Bse1I	ACTGG	3 cut(s)	633, 1137, 1385
Bse8I	GATNNNNATC	1 cut(s)	693
BseBI	CCWGG	4 cut(s)	100, 376, 422, 753
BseDI	CCNNGG	7 cut(s)	98, 451, 553, 574, 752, 1511, 1662
BseGI	GGATG	2 cut(s)	1327, 1657
BseJI	GATNNNNATC	1 cut(s)	693
BseLI	CCNNNNNNNGG	5 cut(s)	559, 664, 704, 768, 1148
BseMII	CTCAG	3 cut(s)	759, 854, 1097
BseNI	ACTGG	3 cut(s)	633, 1137, 1385
BsePI	GCGCGC	1 cut(s)	334
BseRI	GAGGAG	2 cut(s)	551, 1679
BseX3I	CGGCCG	1 cut(s)	1611
BseYI	CCCAGC	1 cut(s)	1669
BsgI	GTGCAG	3 cut(s)	4, 1247, 1436
Bsh1236I	CGCG	3 cut(s)	172, 334, 336
Bsh1285I	CGRYCG	1 cut(s)	1614
BshFI	GGCC	4 cut(s)	379, 757, 1185, 1613
BshNI	GGYRCC	1 cut(s)	1073
BsiEI	CGRYCG	1 cut(s)	1614
BsiHKAI	GWGCWC	3 cut(s)	1115, 1241, 1530
BsiHKCI	CYCGRG	2 cut(s)	458, 1547
BsiSI	CCGG	1 cut(s)	15
BslFI	GGGAC	1 cut(s)	1530
BslI	CCNNNNNNNGG	5 cut(s)	559, 664, 704, 768, 1148
BsmAI	GTCTC	2 cut(s)	543, 748
BsmFI	GGGAC	1 cut(s)	1530
BsmI	GAATGC	2 cut(s)	564, 1032
BsnI	GGCC	4 cut(s)	379, 757, 1185, 1613
Bso31I	GGTCTC	2 cut(s)	543, 748
BsoBI	CYCGRG	2 cut(s)	458, 1547
Bsp1286I	GDGCHC	3 cut(s)	1115, 1241, 1530
Bsp143I	GATC	5 cut(s)	469, 688, 694, 812, 1105
Bsp19I	CCATGG	1 cut(s)	1511
BspANI	GGCC	4 cut(s)	379, 757, 1185, 1613
BspCNI	CTCAG	3 cut(s)	758, 853, 1096
BspFNI	CGCG	3 cut(s)	172, 334, 336
BspHI	TCATGA	1 cut(s)	982
BspLI	GGNNCC	7 cut(s)	168, 457, 540, 675, 1075, 1140, 1233
BspMI	ACCTGC	2 cut(s)	412, 1219
BspPI	GGATC	3 cut(s)	464, 689, 820
BspQI	GCTCTTC	1 cut(s)	1402
BspT107I	GGYRCC	1 cut(s)	1073
BspTNI	GGTCTC	2 cut(s)	543, 748
BsrFI	RCCGGY	1 cut(s)	14
BsrI	ACTGG	3 cut(s)	633, 1137, 1385
BssAI	RCCGGY	1 cut(s)	14
BssECI	CCNNGG	7 cut(s)	98, 451, 553, 574, 752, 1511, 1662
BssHII	GCGCGC	1 cut(s)	334
BssMI	GATC	5 cut(s)	469, 688, 694, 812, 1105
BssNI	GRCGYC	1 cut(s)	1074
BssT1I	CCWWGG	3 cut(s)	451, 574, 1511
Bst2UI	CCWGG	4 cut(s)	100, 376, 422, 753
Bst4CI	ACNGT	4 cut(s)	224, 1318, 1403, 1557
Bst6I	CTCTTC	2 cut(s)	594, 1402
BstACI	GRCGYC	1 cut(s)	1074
BstAPI	GCANNNNNTGC	1 cut(s)	1445
BstBAI	YACGTR	2 cut(s)	701, 713
BstC8I	GCNNGC	7 cut(s)	336, 728, 1187, 1241, 1308, 1345, 1659
BstDEI	CTNAG	6 cut(s)	191, 745, 840, 949, 1028, 1083
BstDSI	CCRYGG	1 cut(s)	1511
BstEII	GGTNACC	1 cut(s)	526
BstENI	CCTNNNNNAGG	1 cut(s)	557
BstF5I	GGATG	2 cut(s)	1327, 1657
BstFNI	CGCG	3 cut(s)	172, 334, 336
BstH2I	RGCGCY	2 cut(s)	1077, 1425
BstHHI	GCGC	4 cut(s)	336, 338, 1076, 1424
BstKTI	GATC	5 cut(s)	472, 691, 697, 815, 1108
BstMAI	GTCTC	2 cut(s)	543, 748
BstMBI	GATC	5 cut(s)	469, 688, 694, 812, 1105
BstMCI	CGRYCG	1 cut(s)	1614
BstMWI	GCNNNNNNNGC	7 cut(s)	27, 126, 246, 249, 296, 1154, 1445
BstNI	CCWGG	4 cut(s)	100, 376, 422, 753
BstNSI	RCATGY	1 cut(s)	1310
BstPAI	GACNNNNGTC	1 cut(s)	1501
BstPI	GGTNACC	1 cut(s)	526
BstSCI	CCNGG	4 cut(s)	98, 374, 420, 751
BstUI	CGCG	3 cut(s)	172, 334, 336
BstX2I	RGATCY	2 cut(s)	688, 694
BstYI	RGATCY	2 cut(s)	688, 694
BstZI	CGGCCG	1 cut(s)	1611
BsuRI	GGCC	4 cut(s)	379, 757, 1185, 1613
BtgI	CCRYGG	1 cut(s)	1511
BtgZI	GCGATG	1 cut(s)	69
BtsCI	GGATG	2 cut(s)	1327, 1657
BtsIMutI	CAGTG	2 cut(s)	1130, 1392
BveI	ACCTGC	2 cut(s)	412, 1219
Cac8I	GCNNGC	7 cut(s)	336, 728, 1187, 1241, 1308, 1345, 1659
CciI	TCATGA	1 cut(s)	982
CfoI	GCGC	4 cut(s)	336, 338, 1076, 1424
Cfr10I	RCCGGY	1 cut(s)	14
Cfr13I	GGNCC	6 cut(s)	401, 455, 707, 755, 1139, 1496
Csp6I	GTAC	3 cut(s)	409, 652, 1628
CviAII	CATG	5 cut(s)	117, 983, 1307, 1471, 1512
CviQI	GTAC	3 cut(s)	409, 652, 1628
DdeI	CTNAG	6 cut(s)	191, 745, 840, 949, 1028, 1083
DinI	GGCGCC	1 cut(s)	1075
DpnI	GATC	5 cut(s)	471, 690, 696, 814, 1107
DpnII	GATC	5 cut(s)	469, 688, 694, 812, 1105
DraI	TTTAAA	2 cut(s)	436, 1090
DraIII	CACNNNGTG	1 cut(s)	704
EaeI	YGGCCR	1 cut(s)	1611
EagI	CGGCCG	1 cut(s)	1611
Eam1104I	CTCTTC	2 cut(s)	594, 1402
EarI	CTCTTC	2 cut(s)	594, 1402
EciI	GGCGGA	2 cut(s)	846, 1509
EclXI	CGGCCG	1 cut(s)	1611
Eco130I	CCWWGG	3 cut(s)	451, 574, 1511
Eco31I	GGTCTC	2 cut(s)	543, 748
Eco47I	GGWCC	5 cut(s)	401, 455, 707, 1139, 1496
Eco52I	CGGCCG	1 cut(s)	1611
Eco57I	CTGAAG	1 cut(s)	654
Eco72I	CACGTG	2 cut(s)	701, 713
Eco88I	CYCGRG	2 cut(s)	458, 1547
Eco91I	GGTNACC	1 cut(s)	526
EcoNI	CCTNNNNNAGG	1 cut(s)	557
EcoO109I	RGGNCCY	1 cut(s)	1139
EcoO65I	GGTNACC	1 cut(s)	526
EcoRI	GAATTC	1 cut(s)	109
EcoRII	CCWGG	4 cut(s)	98, 374, 420, 751
EcoT14I	CCWWGG	3 cut(s)	451, 574, 1511
EcoT22I	ATGCAT	2 cut(s)	1300, 1308
EgeI	GGCGCC	1 cut(s)	1075
EheI	GGCGCC	1 cut(s)	1075
ErhI	CCWWGG	3 cut(s)	451, 574, 1511
FaeI	CATG	5 cut(s)	120, 986, 1310, 1474, 1515
FalI	AAGNNNNNCTT	2 cut(s)	1011, 1043
FaqI	GGGAC	1 cut(s)	1530
FatI	CATG	5 cut(s)	116, 982, 1306, 1470, 1511
FauI	CCCGC	3 cut(s)	68, 245, 1129
FauNDI	CATATG	2 cut(s)	955, 1532
FblI	GTMKAC	3 cut(s)	318, 625, 1122
FokI	GGATG	2 cut(s)	1314, 1664
FspBI	CTAG	4 cut(s)	75, 207, 692, 1442
GlaI	GCGC	4 cut(s)	335, 337, 1075, 1423
GsaI	CCCAGC	1 cut(s)	1673
HaeII	RGCGCY	2 cut(s)	1077, 1425
HaeIII	GGCC	4 cut(s)	379, 757, 1185, 1613
HapII	CCGG	1 cut(s)	15
HhaI	GCGC	4 cut(s)	336, 338, 1076, 1424
Hin1I	GRCGYC	1 cut(s)	1074
Hin1II	CATG	5 cut(s)	120, 986, 1310, 1474, 1515
Hin6I	GCGC	4 cut(s)	334, 336, 1074, 1422
HinP1I	GCGC	4 cut(s)	334, 336, 1074, 1422
HincII	GTYRAC	5 cut(s)	273, 427, 898, 1123, 1359
HindII	GTYRAC	5 cut(s)	273, 427, 898, 1123, 1359
HinfI	GANTC	7 cut(s)	278, 350, 428, 935, 1269, 1518, 1543
HpaII	CCGG	1 cut(s)	15
HphI	GGTGA	9 cut(s)	4, 40, 230, 236, 313, 384, 538, 569, 880
Hpy166II	GTNNAC	8 cut(s)	273, 319, 427, 626, 710, 898, 1123, 1359
Hpy188I	TCNGA	6 cut(s)	355, 748, 843, 1110, 1236, 1563
Hpy188III	TCNNGA	5 cut(s)	692, 983, 1103, 1397, 1547
Hpy8I	GTNNAC	8 cut(s)	273, 319, 427, 626, 710, 898, 1123, 1359
Hpy99I	CGWCG	3 cut(s)	289, 295, 313
HpyAV	CCTTC	3 cut(s)	289, 457, 809
HpyCH4III	ACNGT	4 cut(s)	224, 1318, 1403, 1557
HpyCH4IV	ACGT	4 cut(s)	622, 700, 712, 1586
HpyCH4V	TGCA	8 cut(s)	21, 683, 836, 1228, 1298, 1306, 1417, 1439
HpyF10VI	GCNNNNNNNGC	7 cut(s)	27, 126, 246, 249, 296, 1154, 1445
HpyF3I	CTNAG	6 cut(s)	191, 745, 840, 949, 1028, 1083
HpySE526I	ACGT	4 cut(s)	622, 700, 712, 1586
Hsp92I	GRCGYC	1 cut(s)	1074
Hsp92II	CATG	5 cut(s)	120, 986, 1310, 1474, 1515
HspAI	GCGC	4 cut(s)	334, 336, 1074, 1422
KasI	GGCGCC	1 cut(s)	1073
Kzo9I	GATC	5 cut(s)	469, 688, 694, 812, 1105
LguI	GCTCTTC	1 cut(s)	1402
LmnI	GCTCC	3 cut(s)	538, 1110, 1666
LweI	GCATC	2 cut(s)	845, 1307
MaeI	CTAG	4 cut(s)	75, 207, 692, 1442
MaeII	ACGT	4 cut(s)	622, 700, 712, 1586
MaeIII	GTNAC	9 cut(s)	218, 224, 301, 526, 637, 644, 1503, 1570, 1603
MalI	GATC	5 cut(s)	471, 690, 696, 814, 1107
MboI	GATC	5 cut(s)	469, 688, 694, 812, 1105
MfeI	CAATTG	2 cut(s)	807, 1599
MflI	RGATCY	2 cut(s)	688, 694
MhlI	GDGCHC	3 cut(s)	1115, 1241, 1530
Mly113I	GGCGCC	1 cut(s)	1074
MlyI	GAGTC	6 cut(s)	272, 344, 422, 1263, 1512, 1537
MmeI	TCCRAC	2 cut(s)	643, 1561
Mph1103I	ATGCAT	2 cut(s)	1300, 1308
MroXI	GAANNNNTTC	1 cut(s)	875
MseI	TTAA	4 cut(s)	435, 492, 1089, 1374
MslI	CAYNNNNRTG	1 cut(s)	1510
MspA1I	CMGCKG	2 cut(s)	252, 1610
MspI	CCGG	1 cut(s)	15
MspR9I	CCNGG	4 cut(s)	100, 376, 422, 753
MunI	CAATTG	2 cut(s)	807, 1599
Mva1269I	GAATGC	2 cut(s)	564, 1032
MvaI	CCWGG	4 cut(s)	100, 376, 422, 753
MvnI	CGCG	3 cut(s)	172, 334, 336
MwoI	GCNNNNNNNGC	7 cut(s)	27, 126, 246, 249, 296, 1154, 1445
NarI	GGCGCC	1 cut(s)	1074
NcoI	CCATGG	1 cut(s)	1511
NdeI	CATATG	2 cut(s)	955, 1532
NdeII	GATC	5 cut(s)	469, 688, 694, 812, 1105
NlaIII	CATG	5 cut(s)	120, 986, 1310, 1474, 1515
NlaIV	GGNNCC	7 cut(s)	168, 457, 540, 675, 1075, 1140, 1233
NmeAIII	GCCGAG	1 cut(s)	1687
NmuCI	GTSAC	6 cut(s)	218, 224, 301, 526, 637, 1503
NsiI	ATGCAT	2 cut(s)	1300, 1308
NspI	RCATGY	1 cut(s)	1310
PaeI	GCATGC	1 cut(s)	1310
PagI	TCATGA	1 cut(s)	982
PauI	GCGCGC	1 cut(s)	334
PciSI	GCTCTTC	1 cut(s)	1402
PcsI	WCGNNNNNNNCGW	1 cut(s)	290
PctI	GAATGC	2 cut(s)	564, 1032
PdmI	GAANNNNTTC	1 cut(s)	875
PfeI	GAWTC	1 cut(s)	935
PflMI	CCANNNNNTGG	2 cut(s)	704, 768
PleI	GAGTC	6 cut(s)	272, 344, 422, 1263, 1512, 1537
PluTI	GGCGCC	1 cut(s)	1077
PmaCI	CACGTG	2 cut(s)	701, 713
PmlI	CACGTG	2 cut(s)	701, 713
PpsI	GAGTC	6 cut(s)	272, 344, 422, 1263, 1512, 1537
Ppu21I	YACGTR	2 cut(s)	701, 713
PpuMI	RGGWCCY	1 cut(s)	1139
PshAI	GACNNNNGTC	1 cut(s)	1501
Psp5II	RGGWCCY	1 cut(s)	1139
Psp6I	CCWGG	4 cut(s)	98, 374, 420, 751
PspCI	CACGTG	2 cut(s)	701, 713
PspEI	GGTNACC	1 cut(s)	526
PspFI	CCCAGC	1 cut(s)	1669
PspGI	CCWGG	4 cut(s)	98, 374, 420, 751
PspN4I	GGNNCC	7 cut(s)	168, 457, 540, 675, 1075, 1140, 1233
PspPI	GGNCC	6 cut(s)	401, 455, 707, 755, 1139, 1496
PspPPI	RGGWCCY	1 cut(s)	1139
PsuI	RGATCY	2 cut(s)	688, 694
PteI	GCGCGC	1 cut(s)	334
RsaI	GTAC	3 cut(s)	410, 653, 1629
RsaNI	GTAC	3 cut(s)	409, 652, 1628
RseI	CAYNNNNRTG	1 cut(s)	1510
SalI	GTCGAC	1 cut(s)	1121
SapI	GCTCTTC	1 cut(s)	1402
SaqAI	TTAA	4 cut(s)	435, 492, 1089, 1374
Sau3AI	GATC	5 cut(s)	469, 688, 694, 812, 1105
Sau96I	GGNCC	6 cut(s)	401, 455, 707, 755, 1139, 1496
SchI	GAGTC	6 cut(s)	272, 344, 422, 1263, 1512, 1537
ScrFI	CCNGG	4 cut(s)	100, 376, 422, 753
SduI	GDGCHC	3 cut(s)	1115, 1241, 1530
SfaNI	GCATC	2 cut(s)	845, 1307
SfoI	GGCGCC	1 cut(s)	1075
SinI	GGWCC	5 cut(s)	401, 455, 707, 1139, 1496
SmiMI	CAYNNNNRTG	1 cut(s)	1510
SpeI	ACTAGT	1 cut(s)	1441
SphI	GCATGC	1 cut(s)	1310
SspDI	GGCGCC	1 cut(s)	1073
SspI	AATATT	1 cut(s)	1054
SspMI	CTAG	4 cut(s)	75, 207, 692, 1442
StyD4I	CCNGG	4 cut(s)	98, 374, 420, 751
StyI	CCWWGG	3 cut(s)	451, 574, 1511
TaaI	ACNGT	4 cut(s)	224, 1318, 1403, 1557
TaiI	ACGT	4 cut(s)	625, 703, 715, 1589
TaqI	TCGA	2 cut(s)	36, 1122
TatI	WGTACW	2 cut(s)	651, 1627
TauI	GCSGC	4 cut(s)	66, 132, 382, 1613
TfiI	GAWTC	1 cut(s)	935
Tru1I	TTAA	4 cut(s)	435, 492, 1089, 1374
Tru9I	TTAA	4 cut(s)	435, 492, 1089, 1374
TscAI	CASTG	2 cut(s)	1137, 1392
TseFI	GTSAC	6 cut(s)	218, 224, 301, 526, 637, 1503
Tsp45I	GTSAC	6 cut(s)	218, 224, 301, 526, 637, 1503
TspDTI	ATGAA	5 cut(s)	105, 971, 999, 1033, 1316
TspGWI	ACGGA	2 cut(s)	243, 326
TspRI	CASTG	2 cut(s)	1137, 1392
Van91I	CCANNNNNTGG	2 cut(s)	704, 768
VpaK11BI	GGWCC	5 cut(s)	401, 455, 707, 1139, 1496
XagI	CCTNNNNNAGG	1 cut(s)	557
XapI	RAATTY	6 cut(s)	69, 109, 156, 178, 580, 614
XbaI	TCTAGA	1 cut(s)	691
XceI	RCATGY	1 cut(s)	1310
XcmI	CCANNNNNNNNNTGG	3 cut(s)	765, 1219, 1477
XmiI	GTMKAC	3 cut(s)	318, 625, 1122
XmnI	GAANNNNTTC	1 cut(s)	875
XspI	CTAG	4 cut(s)	75, 207, 692, 1442
Zsp2I	ATGCAT	2 cut(s)	1300, 1308

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.