Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0011472

Physical Location & Seq

Genomic Coordinates Reverse (-)

995616 .. 1000217

Gene Structure 4602 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1009600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1689 bp

ATGGGGACGCTCACCAGCTGCACTTTCAGCACCCTGGATTTCAACTTCACCCTCAAAACCCGCGAAATTCCGAGACTGCCATTTCCCAGGAAACCCAGCAGCAAGAAGAATTCATGCTTCTTTTGCAGCAGAGCGAGTGTAAATACCCAGAGCATTTGTTGGGTTCCGCGTAGGAATTTTGGTAGGTTTAAATGTCATAGTTCTAGCGATAATGGTGACGGTGAAGGTGATGGAGAGAGTAGCAGCGGCAGCGGTAGTAGTAGAAATGAGTCAACAGACCCCACGACGGCGGCGACGACAACGGAAGTAGATGAAGATAATATACGCTCGAACAAATATGATTCCGAGCGTCCATCGGTTTCTTCCAGGCCGCCAACTATATCACCACTTGGGCCTGCGTACAATAATTTCCAAGTTGACTCTTTTAAACTGATGGAACTCCTTGGACCTGAGAAGGTTGATCCTGCTGATGTAAAGCTGATTAAGGAAGCACTTTTTGGTTATTCTACCTTTTGGGTGACCAAAGAGGAGCCTTTCGGAGACCTTGGTGAGGGCATTCTCTTCCTTGGGAATTTGAGGGGAAAGAGAGAAGAGGTATTTGCCAAATTCCAGAGTCTATTGGCTGAGGTCACGGGTAACAAGTACAACCTTTTTATGGTGGAGGAACCCAATGCAGAAGACCTAGATCCACGTGGTGGGCCACGTGTTAGCTTTGGTTTGCTGCGGAAAGAGGTCTCAGAACCAGGGCCAACAACGCTTTGGCAATATGTAATTGCCTTCTTATTATTCCTTCTAACAATTGGATCATCTGTGGAATTAGGAATTGCATCTCAGATAAATAGACTTCCACCAGAGGTGGTGAAATACTTCACAGATCCAAATGCCGTTGACCCACCAGATATGGAGTTGCTATTCCCGTATGTAGATTCAGCCTTGCCTGTAGCATATGGTGTTTTGGGCGTTTTACTGTTTCATGAAGTTGGGCATTTTGTGGCTGCTTTCCCAAAGAAAGTAAAACTTAGCATTCCTTTCTTTATTCCTAATATTACTCTTGGCAGCTTCGGTTCAATCACTCAGTTTAAATCCATTCTTCCTGACCGGAGCACAAAAGTCGACATATCACTGGCGGGCCCTTTTGCTGGTGCTACACTGTCATTGTCAATGTTTGCTGCAGGTCTGCTGCTCTCGTCTAATCCCAACGCTACAGGAGAGTTGGTGCAGGTTCCTAGCATGCTGTTTCAAGGTTCTTTGCTTCTTGGACTCATCAGTAGAGCCACTCTTGGTTATGCATCAATGCATGCTGCGACAGTTCCAATTCATCCTCTTATGATTGCTGGCTGGTGTGGGTTGACAACAACTGCTTTTAATATGCTTCCAGTGGGCTGTCTTGATGGTGGAAGAGCTGTGCAGGGCGCTTTTGGAAAAAATGCTCTAAATGCTTTTGGCTTGACGACATACACGATGCTTGGATTGGGAGTGCTTGGCGGACCTCTCTCACTTCCATGGGGACTCTACGTGCTCATATGCCAGAGGACTCCGGAGAAACCGTGCCTGAATGATGTAACCGAGGTTGGAACGTGGAGGAAAACAGTTGTTACAGTGGCCGTTTTCCTTGTTGTGCTCACACTTCTTCCGGTATGGGATGAGCTTGCCGAGGAGCTAGGTATAGGTCTTGTAACCACATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006091 GO:0006508 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009651 GO:0009657 GO:0009658 GO:0009668 GO:0009719 GO:0009723 GO:0009725 GO:0009959 GO:0009987 GO:0010027 GO:0010033 GO:0010207 GO:0015979 GO:0016043 GO:0016787 GO:0019538 GO:0019684 GO:0022607 GO:0034622 GO:0042221 GO:0043157 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0048564 GO:0050896 GO:0060359 GO:0061024 GO:0065003 GO:0070011 GO:0071704 GO:0071840 GO:0140096 GO:1901564 GO:1901698

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M50

Protein Analysis

563

Amino Acids

60.85

Weight (kDa)

5.41

Isoelectric Point (pI)

37.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M50	PF02163	315 - 475	2.3e-17	Peptidase family M50

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011472)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35220
fragaria_vesca	FvH4_1g28520 FvH4_1g28520
malus_domestica	MD12G1009600.v1.1 MD14G1007000.v1.1
prunus_persica	Prupe.7G011600_v2.0.a1 Prupe.7G011600_v2.0.a1
pyrus_communis	pycom12g00740 pycom14g00550
rosa_chinensis	RchiOBHm_Chr3g0496231
rosa_laevigata	RLG00000022545
rosa_multiflora	Rmu_sc0004080.1_g000013 Rmu_ssc0000454.1_g000006
rosa_roxburghii	Rroxscaffold_6G00389320
rosa_rugosa	Rorug03G0280800 Rorug03G0280900
rosa_samantha	Rh3BG364000 Rh3CG360900 Rh3DG363900
rosa_wichuraiana	Rw3G028710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1163, 1210
AccB7I	CCANNNNNTGG	1 cut(s)	695
AccI	GTMKAC	1 cut(s)	1113
AccII	CGCG	2 cut(s)	63, 169
AccIII	TCCGGA	1 cut(s)	1537
AciI	CCGC	9 cut(s)	61, 167, 246, 252, 290, 371, 724, 1127, 1485
AclWI	GGATC	4 cut(s)	455, 680, 811, 869
AcoI	YGGCCR	1 cut(s)	1602
AcsI	RAATTY	5 cut(s)	66, 109, 175, 571, 605
AcvI	CACGTG	2 cut(s)	692, 704
AdeI	CACNNNGTG	1 cut(s)	695
AfaI	GTAC	2 cut(s)	401, 644
AfiI	CCNNNNNNNGG	5 cut(s)	286, 550, 655, 695, 1139
AflIII	ACRYGT	1 cut(s)	703
AgsI	TTSAA	3 cut(s)	43, 1068, 1241
AjnI	CCWGG	4 cut(s)	33, 86, 365, 742
AjuI	GAANNNNNNNTTGG	2 cut(s)	480, 512
AluBI	AGCT	7 cut(s)	18, 478, 711, 1059, 1403, 1648, 1660
AluI	AGCT	7 cut(s)	18, 478, 711, 1059, 1403, 1648, 1660
Alw21I	GWGCWC	3 cut(s)	1106, 1521, 1623
Alw26I	GTCTC	3 cut(s)	67, 534, 739
AlwI	GGATC	4 cut(s)	455, 680, 811, 869
Aor13HI	TCCGGA	1 cut(s)	1537
AoxI	GGCC	6 cut(s)	368, 392, 698, 746, 1129, 1602
ApaI	GGGCCC	1 cut(s)	1133
ApoI	RAATTY	5 cut(s)	66, 109, 175, 571, 605
Asp700I	GAANNNNTTC	1 cut(s)	866
AspLEI	GCGC	1 cut(s)	1415
AspS9I	GGNCC	7 cut(s)	392, 446, 698, 746, 1129, 1130, 1487
AsuHPI	GGTGA	9 cut(s)	4, 40, 227, 233, 239, 375, 529, 560, 871
AvaII	GGWCC	2 cut(s)	446, 1487
BaeGI	GKGCMC	1 cut(s)	1133
BanII	GRGCYC	1 cut(s)	1133
BarI	GAAGNNNNNNTAC	2 cut(s)	306, 338
BbrPI	CACGTG	2 cut(s)	692, 704
BbsI	GAAGAC	1 cut(s)	684
Bbv12I	GWGCWC	3 cut(s)	1106, 1521, 1623
BbvCI	CCTCAGC	1 cut(s)	624
BccI	CCATC	4 cut(s)	224, 361, 427, 1385
BceAI	ACGGC	3 cut(s)	303, 869, 1589
BcgI	CGANNNNNNTGC	2 cut(s)	1093, 1127
BciT130I	CCWGG	4 cut(s)	35, 88, 367, 744
BcoDI	GTCTC	3 cut(s)	67, 534, 739
BfaI	CTAG	4 cut(s)	204, 683, 1227, 1661
BfmI	CTRYAG	3 cut(s)	939, 1170, 1203
BfoI	RGCGCY	1 cut(s)	1416
BfuAI	ACCTGC	2 cut(s)	1163, 1210
Bme1390I	CCNGG	4 cut(s)	35, 88, 367, 744
Bme18I	GGWCC	2 cut(s)	446, 1487
BmgT120I	GGNCC	7 cut(s)	392, 446, 698, 746, 1129, 1130, 1487
BmiI	GGNNCC	5 cut(s)	165, 531, 666, 1131, 1224
BmrFI	CCNGG	4 cut(s)	35, 88, 367, 744
BmsI	GCATC	3 cut(s)	836, 1298, 1452
BpiI	GAAGAC	1 cut(s)	684
Bpu10I	CCTNAGC	1 cut(s)	624
BsaAI	YACGTR	3 cut(s)	692, 704, 1516
BsaI	GGTCTC	2 cut(s)	534, 739
BsaJI	CCNNGG	9 cut(s)	33, 86, 442, 544, 565, 743, 1502, 1566, 1653
BsaWI	WCCGGW	3 cut(s)	1098, 1537, 1633
BsaXI	ACNNNNNCTCC	4 cut(s)	1200, 1230, 1532, 1562
Bsc4I	CCNNNNNNNGG	5 cut(s)	286, 550, 655, 695, 1139
Bse1I	ACTGG	2 cut(s)	1128, 1376
BseAI	TCCGGA	1 cut(s)	1537
BseBI	CCWGG	4 cut(s)	35, 88, 367, 744
BseDI	CCNNGG	9 cut(s)	33, 86, 442, 544, 565, 743, 1502, 1566, 1653
BseGI	GGATG	2 cut(s)	1318, 1648
BseLI	CCNNNNNNNGG	5 cut(s)	286, 550, 655, 695, 1139
BseMII	CTCAG	5 cut(s)	441, 615, 750, 845, 1088
BseNI	ACTGG	2 cut(s)	1128, 1376
BseRI	GAGGAG	2 cut(s)	542, 1670
BseSI	GKGCMC	1 cut(s)	1133
BseYI	CCCAGC	1 cut(s)	95
BsgI	GTGCAG	3 cut(s)	4, 1238, 1427
Bsh1236I	CGCG	2 cut(s)	63, 169
BshFI	GGCC	6 cut(s)	370, 394, 700, 748, 1131, 1604
BsiHKAI	GWGCWC	3 cut(s)	1106, 1521, 1623
BsiSI	CCGG	3 cut(s)	1099, 1538, 1634
BslFI	GGGAC	2 cut(s)	19, 1521
BslI	CCNNNNNNNGG	5 cut(s)	286, 550, 655, 695, 1139
BsmAI	GTCTC	3 cut(s)	67, 534, 739
BsmFI	GGGAC	2 cut(s)	19, 1521
BsmI	GAATGC	2 cut(s)	555, 1023
BsnI	GGCC	6 cut(s)	370, 394, 700, 748, 1131, 1604
Bso31I	GGTCTC	2 cut(s)	534, 739
Bsp120I	GGGCCC	1 cut(s)	1129
Bsp1286I	GDGCHC	4 cut(s)	1106, 1133, 1521, 1623
Bsp13I	TCCGGA	1 cut(s)	1537
Bsp143I	GATC	4 cut(s)	460, 685, 803, 874
Bsp19I	CCATGG	1 cut(s)	1502
BspACI	CCGC	9 cut(s)	61, 167, 246, 252, 290, 371, 724, 1127, 1485
BspANI	GGCC	6 cut(s)	370, 394, 700, 748, 1131, 1604
BspCNI	CTCAG	5 cut(s)	442, 616, 749, 844, 1087
BspEI	TCCGGA	1 cut(s)	1537
BspFNI	CGCG	2 cut(s)	63, 169
BspHI	TCATGA	1 cut(s)	973
BspLI	GGNNCC	5 cut(s)	165, 531, 666, 1131, 1224
BspMAI	CTGCAG	1 cut(s)	1174
BspMI	ACCTGC	2 cut(s)	1163, 1210
BspPI	GGATC	4 cut(s)	455, 680, 811, 869
BspQI	GCTCTTC	1 cut(s)	1393
BspTNI	GGTCTC	2 cut(s)	534, 739
BsrI	ACTGG	2 cut(s)	1128, 1376
BssECI	CCNNGG	9 cut(s)	33, 86, 442, 544, 565, 743, 1502, 1566, 1653
BssMI	GATC	4 cut(s)	460, 685, 803, 874
BssT1I	CCWWGG	4 cut(s)	442, 544, 565, 1502
Bst2UI	CCWGG	4 cut(s)	35, 88, 367, 744
Bst4CI	ACNGT	7 cut(s)	221, 969, 1152, 1309, 1548, 1591, 1600
Bst6I	CTCTTC	3 cut(s)	566, 585, 1393
BstBAI	YACGTR	3 cut(s)	692, 704, 1516
BstC8I	GCNNGC	6 cut(s)	396, 1129, 1232, 1299, 1336, 1650
BstDEI	CTNAG	6 cut(s)	450, 624, 736, 831, 1019, 1074
BstDSI	CCRYGG	1 cut(s)	1502
BstEII	GGTNACC	1 cut(s)	517
BstENI	CCTNNNNNAGG	1 cut(s)	548
BstF5I	GGATG	2 cut(s)	1318, 1648
BstFNI	CGCG	2 cut(s)	63, 169
BstH2I	RGCGCY	1 cut(s)	1416
BstHHI	GCGC	1 cut(s)	1415
BstKTI	GATC	4 cut(s)	463, 688, 806, 877
BstMAI	GTCTC	3 cut(s)	67, 534, 739
BstMBI	GATC	4 cut(s)	460, 685, 803, 874
BstMWI	GCNNNNNNNGC	5 cut(s)	27, 123, 249, 754, 1436
BstNI	CCWGG	4 cut(s)	35, 88, 367, 744
BstNSI	RCATGY	2 cut(s)	1234, 1301
BstPI	GGTNACC	1 cut(s)	517
BstSCI	CCNGG	4 cut(s)	33, 86, 365, 742
BstSFI	CTRYAG	3 cut(s)	939, 1170, 1203
BstSLI	GKGCMC	1 cut(s)	1133
BstUI	CGCG	2 cut(s)	63, 169
BstV2I	GAAGAC	1 cut(s)	684
BstX2I	RGATCY	2 cut(s)	685, 874
BstYI	RGATCY	2 cut(s)	685, 874
BsuRI	GGCC	6 cut(s)	370, 394, 700, 748, 1131, 1604
BtgI	CCRYGG	1 cut(s)	1502
BtsCI	GGATG	2 cut(s)	1318, 1648
BtsIMutI	CAGTG	4 cut(s)	1121, 1148, 1383, 1605
BveI	ACCTGC	2 cut(s)	1163, 1210
Cac8I	GCNNGC	6 cut(s)	396, 1129, 1232, 1299, 1336, 1650
CciI	TCATGA	1 cut(s)	973
CfoI	GCGC	1 cut(s)	1415
Cfr13I	GGNCC	7 cut(s)	392, 446, 698, 746, 1129, 1130, 1487
CseI	GACGC	2 cut(s)	16, 338
Csp6I	GTAC	2 cut(s)	400, 643
CviAII	CATG	5 cut(s)	114, 974, 1231, 1298, 1503
CviQI	GTAC	2 cut(s)	400, 643
DdeI	CTNAG	6 cut(s)	450, 624, 736, 831, 1019, 1074
DpnI	GATC	4 cut(s)	462, 687, 805, 876
DpnII	GATC	4 cut(s)	460, 685, 803, 874
DraI	TTTAAA	3 cut(s)	190, 427, 1081
DraIII	CACNNNGTG	1 cut(s)	695
EaeI	YGGCCR	1 cut(s)	1602
Eam1104I	CTCTTC	3 cut(s)	566, 585, 1393
EarI	CTCTTC	3 cut(s)	566, 585, 1393
EciI	GGCGGA	1 cut(s)	1500
Eco130I	CCWWGG	4 cut(s)	442, 544, 565, 1502
Eco24I	GRGCYC	1 cut(s)	1133
Eco31I	GGTCTC	2 cut(s)	534, 739
Eco47I	GGWCC	2 cut(s)	446, 1487
Eco72I	CACGTG	2 cut(s)	692, 704
Eco91I	GGTNACC	1 cut(s)	517
EcoNI	CCTNNNNNAGG	1 cut(s)	548
EcoO109I	RGGNCCY	1 cut(s)	1130
EcoO65I	GGTNACC	1 cut(s)	517
EcoRI	GAATTC	1 cut(s)	109
EcoRII	CCWGG	4 cut(s)	33, 86, 365, 742
EcoT14I	CCWWGG	4 cut(s)	442, 544, 565, 1502
EcoT22I	ATGCAT	2 cut(s)	1291, 1299
EcoT38I	GRGCYC	1 cut(s)	1133
ErhI	CCWWGG	4 cut(s)	442, 544, 565, 1502
FaeI	CATG	5 cut(s)	117, 977, 1234, 1301, 1506
FalI	AAGNNNNNCTT	2 cut(s)	1002, 1034
FaqI	GGGAC	2 cut(s)	19, 1521
FatI	CATG	5 cut(s)	113, 973, 1230, 1297, 1502
FauI	CCCGC	2 cut(s)	68, 1120
FauNDI	CATATG	2 cut(s)	946, 1523
FblI	GTMKAC	1 cut(s)	1113
FokI	GGATG	2 cut(s)	1305, 1655
FriOI	GRGCYC	1 cut(s)	1133
FspBI	CTAG	4 cut(s)	204, 683, 1227, 1661
GlaI	GCGC	1 cut(s)	1414
GsaI	CCCAGC	1 cut(s)	99
HaeII	RGCGCY	1 cut(s)	1416
HaeIII	GGCC	6 cut(s)	370, 394, 700, 748, 1131, 1604
HapII	CCGG	3 cut(s)	1099, 1538, 1634
HgaI	GACGC	2 cut(s)	16, 338
HhaI	GCGC	1 cut(s)	1415
Hin1II	CATG	5 cut(s)	117, 977, 1234, 1301, 1506
Hin6I	GCGC	1 cut(s)	1413
HinP1I	GCGC	1 cut(s)	1413
HincII	GTYRAC	5 cut(s)	273, 418, 889, 1114, 1350
HindII	GTYRAC	5 cut(s)	273, 418, 889, 1114, 1350
HinfI	GANTC	8 cut(s)	269, 341, 419, 613, 926, 1260, 1509, 1534
HpaII	CCGG	3 cut(s)	1099, 1538, 1634
HphI	GGTGA	9 cut(s)	4, 40, 227, 233, 239, 375, 529, 560, 871
Hpy166II	GTNNAC	5 cut(s)	273, 418, 889, 1114, 1350
Hpy188I	TCNGA	5 cut(s)	72, 346, 539, 739, 834
Hpy188III	TCNNGA	5 cut(s)	610, 974, 1094, 1388, 1538
Hpy8I	GTNNAC	5 cut(s)	273, 418, 889, 1114, 1350
Hpy99I	CGWCG	2 cut(s)	289, 298
HpyAV	CCTTC	4 cut(s)	218, 448, 787, 800
HpyCH4III	ACNGT	7 cut(s)	221, 969, 1152, 1309, 1548, 1591, 1600
HpyCH4IV	ACGT	4 cut(s)	691, 703, 1515, 1577
HpyCH4V	TGCA	9 cut(s)	21, 126, 674, 827, 1172, 1219, 1289, 1297, 1408
HpyF10VI	GCNNNNNNNGC	5 cut(s)	27, 123, 249, 754, 1436
HpyF3I	CTNAG	6 cut(s)	450, 624, 736, 831, 1019, 1074
HpySE526I	ACGT	4 cut(s)	691, 703, 1515, 1577
Hsp92II	CATG	5 cut(s)	117, 977, 1234, 1301, 1506
HspAI	GCGC	1 cut(s)	1413
Kpn2I	TCCGGA	1 cut(s)	1537
Kzo9I	GATC	4 cut(s)	460, 685, 803, 874
LguI	GCTCTTC	1 cut(s)	1393
LmnI	GCTCC	3 cut(s)	529, 1101, 1657
LweI	GCATC	3 cut(s)	836, 1298, 1452
MaeI	CTAG	4 cut(s)	204, 683, 1227, 1661
MaeII	ACGT	4 cut(s)	691, 703, 1515, 1577
MaeIII	GTNAC	7 cut(s)	215, 517, 628, 635, 1561, 1594, 1675
MalI	GATC	4 cut(s)	462, 687, 805, 876
MboI	GATC	4 cut(s)	460, 685, 803, 874
MboII	GAAGA	9 cut(s)	118, 326, 354, 553, 602, 689, 1082, 1410, 1622
MfeI	CAATTG	1 cut(s)	798
MflI	RGATCY	2 cut(s)	685, 874
MhlI	GDGCHC	4 cut(s)	1106, 1133, 1521, 1623
MlyI	GAGTC	6 cut(s)	278, 413, 622, 1254, 1503, 1528
MmeI	TCCRAC	1 cut(s)	1552
Mph1103I	ATGCAT	2 cut(s)	1291, 1299
MroI	TCCGGA	1 cut(s)	1537
MroXI	GAANNNNTTC	1 cut(s)	866
MseI	TTAA	5 cut(s)	189, 426, 483, 1080, 1365
MslI	CAYNNNNRTG	1 cut(s)	1501
MspA1I	CMGCKG	3 cut(s)	18, 246, 252
MspI	CCGG	3 cut(s)	1099, 1538, 1634
MspR9I	CCNGG	4 cut(s)	35, 88, 367, 744
MunI	CAATTG	1 cut(s)	798
Mva1269I	GAATGC	2 cut(s)	555, 1023
MvaI	CCWGG	4 cut(s)	35, 88, 367, 744
MvnI	CGCG	2 cut(s)	63, 169
MwoI	GCNNNNNNNGC	5 cut(s)	27, 123, 249, 754, 1436
NcoI	CCATGG	1 cut(s)	1502
NdeI	CATATG	2 cut(s)	946, 1523
NdeII	GATC	4 cut(s)	460, 685, 803, 874
NlaIII	CATG	5 cut(s)	117, 977, 1234, 1301, 1506
NlaIV	GGNNCC	5 cut(s)	165, 531, 666, 1131, 1224
NmeAIII	GCCGAG	1 cut(s)	1678
NmuCI	GTSAC	3 cut(s)	215, 517, 628
NsiI	ATGCAT	2 cut(s)	1291, 1299
NspI	RCATGY	2 cut(s)	1234, 1301
PaeI	GCATGC	2 cut(s)	1234, 1301
PagI	TCATGA	1 cut(s)	973
PciSI	GCTCTTC	1 cut(s)	1393
PcsI	WCGNNNNNNNCGW	3 cut(s)	290, 293, 1457
PctI	GAATGC	2 cut(s)	555, 1023
PdmI	GAANNNNTTC	1 cut(s)	866
PfeI	GAWTC	2 cut(s)	341, 926
PflMI	CCANNNNNTGG	1 cut(s)	695
PleI	GAGTC	6 cut(s)	277, 413, 621, 1254, 1503, 1528
PmaCI	CACGTG	2 cut(s)	692, 704
PmlI	CACGTG	2 cut(s)	692, 704
PpsI	GAGTC	6 cut(s)	277, 413, 621, 1254, 1503, 1528
Ppu21I	YACGTR	3 cut(s)	692, 704, 1516
Psp6I	CCWGG	4 cut(s)	33, 86, 365, 742
PspCI	CACGTG	2 cut(s)	692, 704
PspEI	GGTNACC	1 cut(s)	517
PspFI	CCCAGC	1 cut(s)	95
PspGI	CCWGG	4 cut(s)	33, 86, 365, 742
PspN4I	GGNNCC	5 cut(s)	165, 531, 666, 1131, 1224
PspOMI	GGGCCC	1 cut(s)	1129
PspPI	GGNCC	7 cut(s)	392, 446, 698, 746, 1129, 1130, 1487
PstI	CTGCAG	1 cut(s)	1174
PsuI	RGATCY	2 cut(s)	685, 874
PvuII	CAGCTG	1 cut(s)	18
RsaI	GTAC	2 cut(s)	401, 644
RsaNI	GTAC	2 cut(s)	400, 643
RseI	CAYNNNNRTG	1 cut(s)	1501
SalI	GTCGAC	1 cut(s)	1112
SapI	GCTCTTC	1 cut(s)	1393
SaqAI	TTAA	5 cut(s)	189, 426, 483, 1080, 1365
Sau3AI	GATC	4 cut(s)	460, 685, 803, 874
Sau96I	GGNCC	7 cut(s)	392, 446, 698, 746, 1129, 1130, 1487
SchI	GAGTC	6 cut(s)	278, 413, 622, 1254, 1503, 1528
ScrFI	CCNGG	4 cut(s)	35, 88, 367, 744
SduI	GDGCHC	4 cut(s)	1106, 1133, 1521, 1623
SfaNI	GCATC	3 cut(s)	836, 1298, 1452
SfcI	CTRYAG	3 cut(s)	939, 1170, 1203
SinI	GGWCC	2 cut(s)	446, 1487
SmiMI	CAYNNNNRTG	1 cut(s)	1501
SphI	GCATGC	2 cut(s)	1234, 1301
SsiI	CCGC	9 cut(s)	61, 167, 246, 252, 290, 371, 724, 1127, 1485
SspI	AATATT	1 cut(s)	1045
SspMI	CTAG	4 cut(s)	204, 683, 1227, 1661
StyD4I	CCNGG	4 cut(s)	33, 86, 365, 742
StyI	CCWWGG	4 cut(s)	442, 544, 565, 1502
TaaI	ACNGT	7 cut(s)	221, 969, 1152, 1309, 1548, 1591, 1600
TaiI	ACGT	4 cut(s)	694, 706, 1518, 1580
TaqI	TCGA	2 cut(s)	329, 1113
TatI	WGTACW	1 cut(s)	642
TauI	GCSGC	3 cut(s)	249, 293, 373
TfiI	GAWTC	2 cut(s)	341, 926
Tru1I	TTAA	5 cut(s)	189, 426, 483, 1080, 1365
Tru9I	TTAA	5 cut(s)	189, 426, 483, 1080, 1365
TscAI	CASTG	4 cut(s)	1128, 1155, 1383, 1605
TseFI	GTSAC	3 cut(s)	215, 517, 628
Tsp45I	GTSAC	3 cut(s)	215, 517, 628
TspDTI	ATGAA	5 cut(s)	102, 327, 962, 990, 1307
TspGWI	ACGGA	1 cut(s)	317
TspRI	CASTG	4 cut(s)	1128, 1155, 1383, 1605
Van91I	CCANNNNNTGG	1 cut(s)	695
VpaK11BI	GGWCC	2 cut(s)	446, 1487
XagI	CCTNNNNNAGG	1 cut(s)	548
XapI	RAATTY	5 cut(s)	66, 109, 175, 571, 605
XceI	RCATGY	2 cut(s)	1234, 1301
XcmI	CCANNNNNNNNNTGG	1 cut(s)	756
XmiI	GTMKAC	1 cut(s)	1113
XmnI	GAANNNNTTC	1 cut(s)	866
XspI	CTAG	4 cut(s)	204, 683, 1227, 1661
Zsp2I	ATGCAT	2 cut(s)	1291, 1299

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.