AT5G39960

KH-domain-like of EngA bacterial GTPase enzymes, C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007120
Physical Location & Seq
Forward (+)
15993953 .. 15997058
3106 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39960.1

Sequence Viewer

Length: 1866 bp
ATGTCTCAGTCACTGGTTCGAGCAATCTCAGCTCGGAAGAATCAATTCAGTCTTGTTTTTTACTATAGATGTCTTTATTCTAAGCTATTGCATTCCCAAGATCAGATTATTGCTTCCAATTGCTCCAATTTTTCATCTTTAGGATTCAATTCAACTGTTAGACATAGAAGTCATCAGAATAGGTTTTGTAGTAATTTGATCTCAGACTTGAATCTAGTTCGATCCACAAATGGGTTTGCCACGGTTTCTGATGAATCAAATGTTGACAATGGTGAGAAACTTGTGGATAAGAAGCCAAAGATTTTTTACAAGAAACCAATTGATTTCACCAAAATAGATGCCAATTTACTACCTACGGTGATGCTTATGGGCAGGCCAAATGTTGGGAAGTCTGCTTTGTATAATCGATTGATCCGGAGGAGGGAGGCTCTTGTTTACAACACTCCTGATGATCATGTGACAAGGGATATAAGAGAAGGGATTGCAAAGTTAGGTGATTTGAGGTTCAATGTGTTGGACTCTGCTGGTATAGAGACTGAGGTTTCTTCGGGTACTATTCTTGGTAGAACTACAGCAATGACGGCTAATGTGCTTGCAAGGACTCAGTTTGCGGTTCTTATTATCGACGTGAGGGCTGGGTTGCATCCACTAGATTTAGAGGTTGGGAAATGGTTGAGGAAGCATGCTCCACAGATTAAGCCCATTGTGGTTATGAATAAATCTGAATCAATCGGCTCTCTTGATGAAGTTGCTTCAGAGGCCCTTGCACTAGGTTTTGGCGAGCCTATAGCTATTTCAGCCGAGACTGGACTTGGTATGACCACACTTTATGAAGTTCTGCGCCCTCTACTAGAGGATTACACGGTCGAAATGTTAAACGATATAGGCAGTCAAGATGATGTTCTGACCGATGAGAATCTCTCTGATGAAATCGACGAGTCCAAGTTGCCATTGCAGTTAGCTATCGTAGGTAAGCCTAATGTTGGGAAGTCAACCTTGCTCAATGCATTGTTGGAAGAAGAGCGTGTATTAGTGGGTCCAGAAGCTGGCCTCACAAGAGATGCTGTGAGAGTTCAGTTTGAGTTTCAGGGTAGGACCGTTTATCTGGTTGATACTGCTGGGTGGTTGGAGAGAACAGAGCGAGACAAAGGACCAGCATCTTTGAGTATCATGCAATCAAGAAAGAGTCTTATGCGGGCACATGTTATCGCGTTAGTTCTCGATGCTGAAGAGATCATAAAAGCTAAATGTAGTATGACACATTCAGAAGTAGTTATCGCTAGACGTGCTGTAGAAGAAGGACGTGGTCTAGTCGTTATCGTTAACAAAATGGATCGTCTAAGAGGTAGAGAGAACTCTGAGATGTACAAGAAGATCAAAGAAGCTGTTCCCATTGAAATTCAGACAGTTATTCCTCAGATAACTGGAATACCAGTTGTGTTTATCTCTGCATTAGAGGGAAGAGGACGTATGGAAGTAATGAAAGAAGTCACTGATACATACAAGAGATGGTGTTCAAGACTTTCCACAGGCCGCCTCAATCGCTGGTTGCGTAAGGTTATGAGCCGGCATTCATGGAAAGACACGGCTTCACAGCCAAAGATCAAGTTCTTTACTCAAGTGAAAGCTCGACCGCCGACTTTTGTAGCGTTTGTGAGCGGTAAAACGCAGTTATTGGAAAGCGACATAAGGTTTCTGACTAGATCATTGAAGGAAGATTTTGATTTAGGCGGGACTCCTATTCGAATCATACAGCGGGTTATTCCTCGTGCACCACCCTCCGGTACAGGTGGTGGTGGAAGCGGAAACAGCTCGAGTCGAGTAGTGCAAAGAACCACCTCAGACAAACGGACTCTCTCGGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

621

Amino Acids

69.14

Weight (kDa)

9.44

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MMR_HSR1 PF01926 119 - 240 3.1e-17 50S ribosome-binding GTPase
FeoB_N PF02421 319 - 495 5.1e-10 Ferrous iron transport protein B
MMR_HSR1 PF01926 320 - 443 8.3e-21 50S ribosome-binding GTPase
GTP_EFTU PF00009 320 - 500 4e-08 Elongation factor Tu GTP binding domain
KH_dom-like PF14714 507 - 586 5.1e-21 KH-domain-like of EngA bacterial GTPase enzymes, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 168
AccB7I CCANNNNNTGG 2 cut(s) 383, 1046
AccBSI CCGCTC 1 cut(s) 1659
AccII CGCG 1 cut(s) 1211
AccIII TCCGGA 1 cut(s) 414
AciI CCGC 8 cut(s) 611, 1195, 1534, 1634, 1659, 1731, 1756, 1803
AclWI GGATC 3 cut(s) 216, 406, 1341
AcsI RAATTY 1 cut(s) 1398
AcuI CTGAAG 2 cut(s) 738, 1248
AfaI GTAC 3 cut(s) 553, 1367, 1786
AfiI CCNNNNNNNGG 6 cut(s) 231, 383, 421, 983, 1046, 1781
AflIII ACRYGT 1 cut(s) 1201
AgsI TTSAA 7 cut(s) 148, 153, 211, 508, 1397, 1518, 1711
AjiI CACGTC 3 cut(s) 628, 1286, 1304
AluBI AGCT 9 cut(s) 32, 85, 791, 962, 1046, 1244, 1385, 1628, 1812
AluI AGCT 9 cut(s) 32, 85, 791, 962, 1046, 1244, 1385, 1628, 1812
Alw21I GWGCWC 1 cut(s) 1774
Alw26I GTCTC 4 cut(s) 9, 527, 797, 1137
Alw44I GTGCAC 1 cut(s) 1770
AlwI GGATC 3 cut(s) 216, 406, 1341
AlwNI CAGNNNCTG 2 cut(s) 13, 1046
Ama87I CYCGRG 1 cut(s) 1813
Aor13HI TCCGGA 1 cut(s) 414
AoxI GGCC 5 cut(s) 374, 759, 1048, 1531, 1860
ApaLI GTGCAC 1 cut(s) 1770
ApoI RAATTY 1 cut(s) 1398
Asp700I GAANNNNTTC 2 cut(s) 44, 1386
AspLEI GCGC 1 cut(s) 843
AspS9I GGNCC 4 cut(s) 760, 1037, 1095, 1151
AsuHPI GGTGA 4 cut(s) 284, 319, 370, 506
AsuII TTCGAA 1 cut(s) 1744
AvaI CYCGRG 1 cut(s) 1813
AvaII GGWCC 3 cut(s) 1037, 1095, 1151
BaeGI GKGCMC 2 cut(s) 1201, 1774
BauI CACGAG 1 cut(s) 1767
Bbv12I GWGCWC 1 cut(s) 1774
BccI CCATC 1 cut(s) 1503
BceAI ACGGC 2 cut(s) 597, 1602
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 4 cut(s) 9, 527, 797, 1137
BfaI CTAG 7 cut(s) 215, 650, 770, 851, 1281, 1310, 1701
BfmI CTRYAG 4 cut(s) 64, 570, 786, 1290
BisI GCNGC 1 cut(s) 1534
BlsI GCNGC 1 cut(s) 1535
Bme18I GGWCC 3 cut(s) 1037, 1095, 1151
BmeT110I CYCGRG 1 cut(s) 1813
BmgBI CACGTC 3 cut(s) 628, 1286, 1304
BmgT120I GGNCC 4 cut(s) 760, 1037, 1095, 1151
BmiI GGNNCC 1 cut(s) 1038
BmsI GCATC 6 cut(s) 328, 351, 652, 1051, 1166, 1213
BplI GAGNNNNNCTC 4 cut(s) 412, 444, 905, 937
Bpu14I TTCGAA 1 cut(s) 1744
BpuEI CTTGAG 1 cut(s) 1602
Bsa29I ATCGAT 1 cut(s) 406
BsaBI GATNNNNATC 1 cut(s) 915
BsaJI CCNNGG 1 cut(s) 240
BsaWI WCCGGW 2 cut(s) 414, 1781
Bsc4I CCNNNNNNNGG 6 cut(s) 231, 383, 421, 983, 1046, 1781
Bse118I RCCGGY 1 cut(s) 1566
Bse1I ACTGG 4 cut(s) 18, 811, 1429, 1433
Bse3DI GCAATG 2 cut(s) 582, 950
Bse8I GATNNNNATC 1 cut(s) 915
BseAI TCCGGA 1 cut(s) 414
BseCI ATCGAT 1 cut(s) 406
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 1 cut(s) 643
BseJI GATNNNNATC 1 cut(s) 915
BseLI CCNNNNNNNGG 6 cut(s) 231, 383, 421, 983, 1046, 1781
BseMI GCAATG 2 cut(s) 582, 950
BseMII CTCAG 8 cut(s) 20, 42, 216, 528, 617, 1350, 1430, 1854
BseNI ACTGG 4 cut(s) 18, 811, 1429, 1433
BseRI GAGGAG 1 cut(s) 433
BseSI GKGCMC 2 cut(s) 1201, 1774
BseYI CCCAGC 2 cut(s) 635, 1118
Bsh1236I CGCG 1 cut(s) 1211
Bsh1285I CGRYCG 2 cut(s) 867, 1634
BshFI GGCC 5 cut(s) 376, 761, 1050, 1533, 1862
BshVI ATCGAT 1 cut(s) 406
BsiEI CGRYCG 2 cut(s) 867, 1634
BsiHKAI GWGCWC 1 cut(s) 1774
BsiHKCI CYCGRG 1 cut(s) 1813
BsiSI CCGG 3 cut(s) 415, 1567, 1782
BslFI GGGAC 1 cut(s) 1747
BslI CCNNNNNNNGG 6 cut(s) 231, 383, 421, 983, 1046, 1781
BsmAI GTCTC 4 cut(s) 9, 527, 797, 1137
BsmFI GGGAC 1 cut(s) 1747
BsmI GAATGC 2 cut(s) 91, 1570
BsnI GGCC 5 cut(s) 376, 761, 1050, 1533, 1862
BsoBI CYCGRG 1 cut(s) 1813
Bsp119I TTCGAA 1 cut(s) 1744
Bsp1286I GDGCHC 2 cut(s) 1201, 1774
Bsp13I TCCGGA 1 cut(s) 414
Bsp1407I TGTACA 1 cut(s) 1365
BspACI CCGC 8 cut(s) 611, 1195, 1534, 1634, 1659, 1731, 1756, 1803
BspANI GGCC 5 cut(s) 376, 761, 1050, 1533, 1862
BspCNI CTCAG 8 cut(s) 19, 41, 215, 529, 616, 1351, 1429, 1853
BspDI ATCGAT 1 cut(s) 406
BspEI TCCGGA 1 cut(s) 414
BspFNI CGCG 1 cut(s) 1211
BspLI GGNNCC 1 cut(s) 1038
BspPI GGATC 3 cut(s) 216, 406, 1341
BspQI GCTCTTC 1 cut(s) 1014
BspT104I TTCGAA 1 cut(s) 1744
BsrBI CCGCTC 1 cut(s) 1659
BsrDI GCAATG 2 cut(s) 582, 950
BsrFI RCCGGY 1 cut(s) 1566
BsrGI TGTACA 1 cut(s) 1365
BsrI ACTGG 4 cut(s) 18, 811, 1429, 1433
BssAI RCCGGY 1 cut(s) 1566
BssECI CCNNGG 1 cut(s) 240
BssSI CACGAG 1 cut(s) 1767
Bst2BI CACGAG 1 cut(s) 1767
Bst4CI ACNGT 6 cut(s) 157, 244, 358, 865, 1099, 1408
Bst6I CTCTTC 3 cut(s) 1014, 1224, 1456
BstAUI TGTACA 1 cut(s) 1365
BstBI TTCGAA 1 cut(s) 1744
BstC8I GCNNGC 7 cut(s) 374, 594, 684, 782, 1048, 1197, 1568
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 1 cut(s) 643
BstFNI CGCG 1 cut(s) 1211
BstHHI GCGC 1 cut(s) 843
BstMAI GTCTC 4 cut(s) 9, 527, 797, 1137
BstMCI CGRYCG 2 cut(s) 867, 1634
BstMWI GCNNNNNNNGC 7 cut(s) 29, 581, 758, 797, 1286, 1542, 1809
BstNSI RCATGY 2 cut(s) 686, 1205
BstSFI CTRYAG 4 cut(s) 64, 570, 786, 1290
BstSLI GKGCMC 2 cut(s) 1201, 1774
BstUI CGCG 1 cut(s) 1211
Bsu15I ATCGAT 1 cut(s) 406
BsuRI GGCC 5 cut(s) 376, 761, 1050, 1533, 1862
BsuTUI ATCGAT 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 240
BtrI CACGTC 3 cut(s) 628, 1286, 1304
BtsCI GGATG 1 cut(s) 643
BtsIMutI CAGTG 2 cut(s) 11, 1491
Cac8I GCNNGC 7 cut(s) 374, 594, 684, 782, 1048, 1197, 1568
CaiI CAGNNNCTG 2 cut(s) 13, 1046
CfoI GCGC 1 cut(s) 843
Cfr10I RCCGGY 1 cut(s) 1566
Cfr13I GGNCC 4 cut(s) 760, 1037, 1095, 1151
ClaI ATCGAT 1 cut(s) 406
Csp6I GTAC 3 cut(s) 552, 1366, 1785
CviAII CATG 5 cut(s) 455, 683, 1171, 1202, 1575
CviQI GTAC 3 cut(s) 552, 1366, 1785
DrdI GACNNNNNNGTC 1 cut(s) 168
DseDI GACNNNNNNGTC 1 cut(s) 168
Eam1104I CTCTTC 3 cut(s) 1014, 1224, 1456
EarI CTCTTC 3 cut(s) 1014, 1224, 1456
Eco47I GGWCC 3 cut(s) 1037, 1095, 1151
Eco57I CTGAAG 2 cut(s) 738, 1248
Eco88I CYCGRG 1 cut(s) 1813
EcoO109I RGGNCCY 1 cut(s) 760
EcoT22I ATGCAT 1 cut(s) 1009
FaeI CATG 5 cut(s) 458, 686, 1174, 1205, 1578
FalI AAGNNNNNCTT 2 cut(s) 980, 1012
FaqI GGGAC 1 cut(s) 1747
FatI CATG 5 cut(s) 454, 682, 1170, 1201, 1574
FauI CCCGC 3 cut(s) 1188, 1724, 1749
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 1 cut(s) 1534
FokI GGATG 1 cut(s) 630
Fsp4HI GCNGC 1 cut(s) 1534
FspBI CTAG 7 cut(s) 215, 650, 770, 851, 1281, 1310, 1701
GlaI GCGC 1 cut(s) 842
GluI GCNGC 1 cut(s) 1534
GsaI CCCAGC 2 cut(s) 639, 1122
HaeIII GGCC 5 cut(s) 376, 761, 1050, 1533, 1862
HapII CCGG 3 cut(s) 415, 1567, 1782
HhaI GCGC 1 cut(s) 843
Hin1II CATG 5 cut(s) 458, 686, 1174, 1205, 1578
Hin6I GCGC 1 cut(s) 841
HinP1I GCGC 1 cut(s) 841
HincII GTYRAC 3 cut(s) 265, 993, 1324
HindII GTYRAC 3 cut(s) 265, 993, 1324
HpaI GTTAAC 1 cut(s) 1324
HpaII CCGG 3 cut(s) 415, 1567, 1782
HphI GGTGA 4 cut(s) 284, 319, 370, 506
Hpy166II GTNNAC 5 cut(s) 265, 436, 993, 1324, 1772
Hpy188III TCNNGA 8 cut(s) 415, 446, 740, 893, 1040, 1179, 1220, 1518
Hpy8I GTNNAC 5 cut(s) 265, 436, 993, 1324, 1772
Hpy99I CGWCG 2 cut(s) 629, 938
HpyAV CCTTC 3 cut(s) 470, 1292, 1705
HpyCH4III ACNGT 6 cut(s) 157, 244, 358, 865, 1099, 1408
HpyCH4IV ACGT 4 cut(s) 627, 1285, 1303, 1468
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 581, 758, 797, 1286, 1542, 1809
HpySE526I ACGT 4 cut(s) 627, 1285, 1303, 1468
Hsp92II CATG 5 cut(s) 458, 686, 1174, 1205, 1578
HspAI GCGC 1 cut(s) 841
Kpn2I TCCGGA 1 cut(s) 414
KroI GCCGGC 1 cut(s) 1566
KroNI GCCGGC 1 cut(s) 1568
Ksp22I TGATCA 1 cut(s) 451
KspAI GTTAAC 1 cut(s) 1324
LguI GCTCTTC 1 cut(s) 1014
LmnI GCTCC 2 cut(s) 128, 691
LweI GCATC 6 cut(s) 328, 351, 652, 1051, 1166, 1213
MaeI CTAG 7 cut(s) 215, 650, 770, 851, 1281, 1310, 1701
MaeII ACGT 4 cut(s) 627, 1285, 1303, 1468
MaeIII GTNAC 3 cut(s) 9, 457, 1489
MbiI CCGCTC 1 cut(s) 1659
MboII GAAGA 9 cut(s) 49, 537, 1028, 1031, 1241, 1307, 1384, 1473, 1727
MfeI CAATTG 2 cut(s) 118, 318
MhlI GDGCHC 2 cut(s) 1201, 1774
MluCI AATT 8 cut(s) 44, 118, 127, 148, 193, 318, 343, 1398
MlyI GAGTC 7 cut(s) 512, 595, 947, 1195, 1729, 1825, 1846
MmeI TCCRAC 3 cut(s) 495, 993, 1107
Mph1103I ATGCAT 1 cut(s) 1009
MroI TCCGGA 1 cut(s) 414
MroNI GCCGGC 1 cut(s) 1566
MroXI GAANNNNTTC 2 cut(s) 44, 1386
MseI TTAA 3 cut(s) 696, 875, 1323
MspA1I CMGCKG 1 cut(s) 1756
MspI CCGG 3 cut(s) 415, 1567, 1782
MunI CAATTG 2 cut(s) 118, 318
Mva1269I GAATGC 2 cut(s) 91, 1570
MvnI CGCG 1 cut(s) 1211
MwoI GCNNNNNNNGC 7 cut(s) 29, 581, 758, 797, 1286, 1542, 1809
NaeI GCCGGC 1 cut(s) 1568
NgoMIV GCCGGC 1 cut(s) 1566
NlaIII CATG 5 cut(s) 458, 686, 1174, 1205, 1578
NlaIV GGNNCC 1 cut(s) 1038
NmeAIII GCCGAG 2 cut(s) 826, 1838
NmuCI GTSAC 3 cut(s) 9, 457, 1489
NsiI ATGCAT 1 cut(s) 1009
NspI RCATGY 2 cut(s) 686, 1205
NspV TTCGAA 1 cut(s) 1744
PaeI GCATGC 1 cut(s) 686
PaeR7I CTCGAG 1 cut(s) 1813
PciI ACATGT 1 cut(s) 1201
PciSI GCTCTTC 1 cut(s) 1014
PcsI WCGNNNNNNNCGW 1 cut(s) 1549
PctI GAATGC 2 cut(s) 91, 1570
PdiI GCCGGC 1 cut(s) 1568
PdmI GAANNNNTTC 2 cut(s) 44, 1386
PfeI GAWTC 7 cut(s) 40, 144, 211, 254, 725, 916, 1746
PflFI GACNNNGTC 1 cut(s) 1305
PflMI CCANNNNNTGG 2 cut(s) 383, 1046
PkrI GCNGC 1 cut(s) 1535
PleI GAGTC 7 cut(s) 512, 595, 946, 1194, 1729, 1824, 1846
PpsI GAGTC 7 cut(s) 512, 595, 946, 1194, 1729, 1824, 1846
PscI ACATGT 1 cut(s) 1201
PspFI CCCAGC 2 cut(s) 635, 1118
PspN4I GGNNCC 1 cut(s) 1038
PspPI GGNCC 4 cut(s) 760, 1037, 1095, 1151
PspXI VCTCGAGB 1 cut(s) 1813
PstNI CAGNNNCTG 2 cut(s) 13, 1046
PsyI GACNNNGTC 1 cut(s) 1305
RsaI GTAC 3 cut(s) 553, 1367, 1786
RsaNI GTAC 3 cut(s) 552, 1366, 1785
SapI GCTCTTC 1 cut(s) 1014
SaqAI TTAA 3 cut(s) 696, 875, 1323
SatI GCNGC 1 cut(s) 1534
Sau96I GGNCC 4 cut(s) 760, 1037, 1095, 1151
SchI GAGTC 7 cut(s) 512, 595, 947, 1195, 1729, 1825, 1846
SduI GDGCHC 2 cut(s) 1201, 1774
SfaNI GCATC 6 cut(s) 328, 351, 652, 1051, 1166, 1213
SfcI CTRYAG 4 cut(s) 64, 570, 786, 1290
Sfr274I CTCGAG 1 cut(s) 1813
SfuI TTCGAA 1 cut(s) 1744
SinI GGWCC 3 cut(s) 1037, 1095, 1151
SlaI CTCGAG 1 cut(s) 1813
SmlI CTYRAG 2 cut(s) 1617, 1813
SmoI CTYRAG 2 cut(s) 1617, 1813
SphI GCATGC 1 cut(s) 686
Sse9I AATT 8 cut(s) 44, 118, 127, 148, 193, 318, 343, 1398
SsiI CCGC 8 cut(s) 611, 1195, 1534, 1634, 1659, 1731, 1756, 1803
SspMI CTAG 7 cut(s) 215, 650, 770, 851, 1281, 1310, 1701
TaaI ACNGT 6 cut(s) 157, 244, 358, 865, 1099, 1408
TaiI ACGT 4 cut(s) 630, 1288, 1306, 1471
TaqII GACCGA 1 cut(s) 923
TasI AATT 8 cut(s) 44, 118, 127, 148, 193, 318, 343, 1398
TatI WGTACW 1 cut(s) 1365
TauI GCSGC 1 cut(s) 1536
TfiI GAWTC 7 cut(s) 40, 144, 211, 254, 725, 916, 1746
Tru1I TTAA 3 cut(s) 696, 875, 1323
Tru9I TTAA 3 cut(s) 696, 875, 1323
TscAI CASTG 2 cut(s) 18, 1498
TseFI GTSAC 3 cut(s) 9, 457, 1489
Tsp45I GTSAC 3 cut(s) 9, 457, 1489
TspDTI ATGAA 8 cut(s) 123, 267, 728, 759, 846, 942, 1496, 1563
TspGWI ACGGA 1 cut(s) 1864
TspRI CASTG 2 cut(s) 18, 1498
Tth111I GACNNNGTC 1 cut(s) 1305
Van91I CCANNNNNTGG 2 cut(s) 383, 1046
VneI GTGCAC 1 cut(s) 1770
VpaK11BI GGWCC 3 cut(s) 1037, 1095, 1151
XapI RAATTY 1 cut(s) 1398
XceI RCATGY 2 cut(s) 686, 1205
XhoI CTCGAG 1 cut(s) 1813
XmnI GAANNNNTTC 2 cut(s) 44, 1386
XspI CTAG 7 cut(s) 215, 650, 770, 851, 1281, 1310, 1701
Zsp2I ATGCAT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.