FvH4_4g04950

purine nucleoside binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007120
Physical Location & Seq
Forward (+)
4283817 .. 4286243
2427 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04950.t1

Sequence Viewer

Length: 1119 bp
ATGAATAAGTCGGAGTCTCTTTTTGATGGTAGTGGGTCACTTGCTGCGGCTGTTGCTGAAGCTTATAAGTTGGGATGGGGTGACCCTATTGCCATTTCAGCTGAAACTGGACTTGGTATGCAAGACCTTTATGGGAGTCTAAAGCCTATGCTTGAGGATTATATACTGAATGTCTTAAACAATGATCAAAACACTGATGAGGGCAATTTCAGTGACGACAGCTCCTGTGAGGTTGAGGATAGTAAGCTGCCCTTACAGTTAGCAATTGTAGGGAAACCCAATGTTGGGAAGTCAACTTTGCTGAATACATTGTTACAAGAAGAATGTGTGCTGGTAGGTCCCGAAGCTGGTCTGACAAGAGATTCAATTAGAGCCGAGTTTGAATTTCAAGGAAGAACTATATACCTGGTTGACACTGCAGGCTGGTTGCAGAGAACAAAGATGGAGAAAGGACCATCATCCTTGAGCATCGTGCAGTCAAGAAAGAGTCTCATGAGAGCTCATGTAGTTGCTCTGGTCCTGGATGCCAAAGAGATCTCAAAGGCCAGAAGAAGTTTGAAGCATGATGAAGTAGTTATAGCTAGACAGGCTATTGAAGAAGGGCGTGGCCTAGTGGTGATTGTGAACAAGATGGATCTAGTGAAAGAACAACAAACTTGTGACAAGGTTATGAAAGCTGTACCTCAAGAAATTCAGACGGTTATACTCCAGGTTACTGGAATACCAGTAGTATTCATATCAGAATTGGAGGGAAGGGGTCAGGCAGCTGTCATGCGCCAGGTTATTGATACATGTGAAAAATGGTGTACAAGGTTGTCCACAGCTCGTCTGAACCGTTGGTTGTGTAAGGTTATGAGCAGGCATTCTTGGAAAGATCAGTCTGCTCAACCCAAGGTTAAGTACTTCACACAGGTGAAAGCACGACCACCTACTTTTGTTGCCTTTGTTAGTGGGAAGAAAGAGCTCCCAGATACAGACGTCAGGTTCTTAAAAAGGTCTTTGAAGGAAGACTTTGATTTGGGTGGAATTCCGATCAGGATCATGCAGAGGAACATCCCACAGAAGACTGCCAATAGCAATAGCAAGAGCAGCCAAATTACTAATAGGATGCCTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_dom-like MMR_HSR1

Protein Analysis

373

Amino Acids

41.34

Weight (kDa)

8.76

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FeoB_N PF02421 86 - 258 2.7e-11 Ferrous iron transport protein B
AIG1 PF04548 86 - 262 1e-06 AIG1 family
MMR_HSR1 PF01926 87 - 210 8.5e-21 50S ribosome-binding GTPase
GTP_EFTU PF00009 115 - 265 2.9e-07 Elongation factor Tu GTP binding domain
KH_dom-like PF14714 271 - 350 2.1e-21 KH-domain-like of EngA bacterial GTPase enzymes, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 66
AatII GACGTC 1 cut(s) 981
AciI CCGC 1 cut(s) 47
AclWI GGATC 2 cut(s) 642, 1046
AcsI RAATTY 3 cut(s) 383, 690, 1026
AcuI CTGAAG 1 cut(s) 78
AcyI GRCGYC 1 cut(s) 978
AfaI GTAC 3 cut(s) 681, 808, 902
AfiI CCNNNNNNNGG 3 cut(s) 284, 285, 347
AflIII ACRYGT 1 cut(s) 791
AgsI TTSAA 6 cut(s) 366, 383, 389, 559, 596, 1003
AjnI CCWGG 4 cut(s) 405, 519, 708, 777
AjuI GAANNNNNNNTTGG 2 cut(s) 96, 128
AleI CACNNNNGTG 1 cut(s) 911
Alw21I GWGCWC 2 cut(s) 502, 966
Alw26I GTCTC 2 cut(s) 21, 494
AlwI GGATC 2 cut(s) 642, 1046
AlwNI CAGNNNCTG 1 cut(s) 225
AoxI GGCC 2 cut(s) 543, 607
ApeKI GCWGC 4 cut(s) 44, 247, 764, 1089
ApoI RAATTY 3 cut(s) 383, 690, 1026
AspLEI GCGC 1 cut(s) 777
AspS9I GGNCC 3 cut(s) 338, 452, 517
AsuHPI GGTGA 3 cut(s) 92, 628, 925
AvaII GGWCC 3 cut(s) 338, 452, 517
BaeI ACNNNNGTAYC 2 cut(s) 963, 996
BanII GRGCYC 2 cut(s) 502, 966
BbsI GAAGAC 2 cut(s) 1014, 1070
Bbv12I GWGCWC 2 cut(s) 502, 966
BbvI GCAGC 4 cut(s) 31, 234, 776, 1101
BccI CCATC 5 cut(s) 20, 69, 436, 463, 625
BciT130I CCWGG 4 cut(s) 407, 521, 710, 779
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 2 cut(s) 21, 494
BfaI CTAG 3 cut(s) 582, 611, 638
BfmI CTRYAG 1 cut(s) 417
BglII AGATCT 1 cut(s) 534
BisI GCNGC 5 cut(s) 45, 48, 248, 765, 1090
BlsI GCNGC 5 cut(s) 46, 49, 249, 766, 1091
BmcAI AGTACT 1 cut(s) 902
Bme1390I CCNGG 4 cut(s) 407, 521, 710, 779
Bme18I GGWCC 3 cut(s) 338, 452, 517
BmgT120I GGNCC 3 cut(s) 338, 452, 517
BmiI GGNNCC 1 cut(s) 340
BmrFI CCNGG 4 cut(s) 407, 521, 710, 779
BmsI GCATC 3 cut(s) 477, 514, 1098
BpiI GAAGAC 2 cut(s) 1014, 1070
BpmI CTGGAG 1 cut(s) 692
BpuEI CTTGAG 3 cut(s) 173, 484, 669
BsaBI GATNNNNATC 1 cut(s) 1037
BsaHI GRCGYC 1 cut(s) 978
BsaJI CCNNGG 1 cut(s) 891
BsaXI ACNNNNNCTCC 2 cut(s) 206, 236
Bsc4I CCNNNNNNNGG 3 cut(s) 284, 285, 347
Bse1I ACTGG 3 cut(s) 112, 721, 725
Bse8I GATNNNNATC 1 cut(s) 1037
BseBI CCWGG 4 cut(s) 407, 521, 710, 779
BseDI CCNNGG 1 cut(s) 891
BseGI GGATG 5 cut(s) 80, 458, 529, 1053, 1113
BseJI GATNNNNATC 1 cut(s) 1037
BseLI CCNNNNNNNGG 3 cut(s) 284, 285, 347
BseNI ACTGG 3 cut(s) 112, 721, 725
BseXI GCAGC 4 cut(s) 31, 234, 776, 1101
BsgI GTGCAG 1 cut(s) 494
BshFI GGCC 2 cut(s) 545, 609
BsiHKAI GWGCWC 2 cut(s) 502, 966
BslFI GGGAC 1 cut(s) 324
BslI CCNNNNNNNGG 3 cut(s) 284, 285, 347
BsmAI GTCTC 2 cut(s) 21, 494
BsmFI GGGAC 1 cut(s) 324
BsmI GAATGC 1 cut(s) 862
BsnI GGCC 2 cut(s) 545, 609
Bsp1286I GDGCHC 2 cut(s) 502, 966
Bsp1407I TGTACA 1 cut(s) 806
Bsp143I GATC 6 cut(s) 184, 534, 634, 874, 1032, 1038
BspACI CCGC 1 cut(s) 47
BspANI GGCC 2 cut(s) 545, 609
BspHI TCATGA 1 cut(s) 492
BspLI GGNNCC 1 cut(s) 340
BspMAI CTGCAG 1 cut(s) 421
BspPI GGATC 2 cut(s) 642, 1046
BsrGI TGTACA 1 cut(s) 806
BsrI ACTGG 3 cut(s) 112, 721, 725
BssECI CCNNGG 1 cut(s) 891
BssMI GATC 6 cut(s) 184, 534, 634, 874, 1032, 1038
BssNI GRCGYC 1 cut(s) 978
BssT1I CCWWGG 1 cut(s) 891
Bst2UI CCWGG 4 cut(s) 407, 521, 710, 779
Bst4CI ACNGT 3 cut(s) 258, 700, 836
BstACI GRCGYC 1 cut(s) 978
BstAUI TGTACA 1 cut(s) 806
BstC8I GCNNGC 2 cut(s) 421, 860
BstEII GGTNACC 1 cut(s) 80
BstF5I GGATG 5 cut(s) 80, 458, 529, 1053, 1113
BstHHI GCGC 1 cut(s) 777
BstKTI GATC 6 cut(s) 187, 537, 637, 877, 1035, 1041
BstMAI GTCTC 2 cut(s) 21, 494
BstMBI GATC 6 cut(s) 184, 534, 634, 874, 1032, 1038
BstMWI GCNNNNNNNGC 4 cut(s) 53, 98, 587, 1089
BstNI CCWGG 4 cut(s) 407, 521, 710, 779
BstNSI RCATGY 1 cut(s) 795
BstPI GGTNACC 1 cut(s) 80
BstSCI CCNGG 4 cut(s) 405, 519, 708, 777
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 4 cut(s) 31, 234, 776, 1101
BstV2I GAAGAC 2 cut(s) 1014, 1070
BstX2I RGATCY 2 cut(s) 534, 634
BstXI CCANNNNNNTGG 1 cut(s) 716
BstYI RGATCY 2 cut(s) 534, 634
BsuRI GGCC 2 cut(s) 545, 609
BtsCI GGATG 5 cut(s) 80, 458, 529, 1053, 1113
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 3 cut(s) 192, 217, 414
Cac8I GCNNGC 2 cut(s) 421, 860
CaiI CAGNNNCTG 1 cut(s) 225
CciI TCATGA 1 cut(s) 492
CfoI GCGC 1 cut(s) 777
Cfr13I GGNCC 3 cut(s) 338, 452, 517
CsiI ACCWGGT 1 cut(s) 405
Csp6I GTAC 3 cut(s) 680, 807, 901
CviAII CATG 6 cut(s) 493, 503, 563, 772, 792, 1042
CviQI GTAC 3 cut(s) 680, 807, 901
DpnI GATC 6 cut(s) 186, 536, 636, 876, 1034, 1040
DpnII GATC 6 cut(s) 184, 534, 634, 874, 1032, 1038
Ecl136II GAGCTC 2 cut(s) 500, 964
Eco130I CCWWGG 1 cut(s) 891
Eco24I GRGCYC 2 cut(s) 502, 966
Eco47I GGWCC 3 cut(s) 338, 452, 517
Eco53kI GAGCTC 2 cut(s) 500, 964
Eco57I CTGAAG 1 cut(s) 78
Eco91I GGTNACC 1 cut(s) 80
EcoICRI GAGCTC 2 cut(s) 500, 964
EcoO109I RGGNCCY 1 cut(s) 338
EcoO65I GGTNACC 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 1026
EcoRII CCWGG 4 cut(s) 405, 519, 708, 777
EcoT14I CCWWGG 1 cut(s) 891
EcoT38I GRGCYC 2 cut(s) 502, 966
ErhI CCWWGG 1 cut(s) 891
FaeI CATG 6 cut(s) 496, 506, 566, 775, 795, 1045
FalI AAGNNNNNCTT 4 cut(s) 236, 268, 995, 1027
FaqI GGGAC 1 cut(s) 324
FatI CATG 6 cut(s) 492, 502, 562, 771, 791, 1041
FbaI TGATCA 1 cut(s) 184
Fnu4HI GCNGC 5 cut(s) 45, 48, 248, 765, 1090
FokI GGATG 4 cut(s) 87, 445, 536, 1040
FriOI GRGCYC 2 cut(s) 502, 966
Fsp4HI GCNGC 5 cut(s) 45, 48, 248, 765, 1090
FspBI CTAG 3 cut(s) 582, 611, 638
GlaI GCGC 1 cut(s) 776
GluI GCNGC 5 cut(s) 45, 48, 248, 765, 1090
GsuI CTGGAG 1 cut(s) 692
HaeIII GGCC 2 cut(s) 545, 609
HhaI GCGC 1 cut(s) 777
Hin1I GRCGYC 1 cut(s) 978
Hin1II CATG 6 cut(s) 496, 506, 566, 775, 795, 1045
Hin6I GCGC 1 cut(s) 775
HinP1I GCGC 1 cut(s) 775
HincII GTYRAC 2 cut(s) 294, 412
HindII GTYRAC 2 cut(s) 294, 412
HindIII AAGCTT 1 cut(s) 60
HinfI GANTC 4 cut(s) 14, 136, 362, 487
HphI GGTGA 3 cut(s) 92, 628, 925
Hpy166II GTNNAC 5 cut(s) 294, 412, 625, 807, 819
Hpy188I TCNGA 6 cut(s) 13, 354, 696, 742, 831, 1032
Hpy188III TCNNGA 5 cut(s) 341, 480, 493, 686, 1036
Hpy8I GTNNAC 5 cut(s) 294, 412, 625, 807, 819
HpyAV CCTTC 3 cut(s) 593, 747, 997
HpyCH4III ACNGT 3 cut(s) 258, 700, 836
HpyCH4IV ACGT 1 cut(s) 978
HpyCH4V TGCA 5 cut(s) 121, 419, 430, 475, 1045
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 98, 587, 1089
HpySE526I ACGT 1 cut(s) 978
Hsp92I GRCGYC 1 cut(s) 978
Hsp92II CATG 6 cut(s) 496, 506, 566, 775, 795, 1045
HspAI GCGC 1 cut(s) 775
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 6 cut(s) 184, 534, 634, 874, 1032, 1038
LmnI GCTCC 2 cut(s) 227, 969
Lsp1109I GCAGC 4 cut(s) 31, 234, 776, 1101
LweI GCATC 3 cut(s) 477, 514, 1098
MabI ACCWGGT 1 cut(s) 405
MaeI CTAG 3 cut(s) 582, 611, 638
MaeII ACGT 1 cut(s) 978
MaeIII GTNAC 6 cut(s) 36, 80, 212, 312, 659, 712
MalI GATC 6 cut(s) 186, 536, 636, 876, 1034, 1040
MboI GATC 6 cut(s) 184, 534, 634, 874, 1032, 1038
MboII GAAGA 7 cut(s) 332, 405, 561, 608, 967, 1019, 1075
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 2 cut(s) 534, 634
MhlI GDGCHC 2 cut(s) 502, 966
MluCI AATT 8 cut(s) 205, 264, 366, 383, 690, 743, 1026, 1095
MlyI GAGTC 3 cut(s) 23, 145, 496
MnlI CCTC 7 cut(s) 148, 193, 223, 229, 693, 742, 1041
MseI TTAA 4 cut(s) 176, 897, 989, 1117
MslI CAYNNNNRTG 1 cut(s) 911
MspA1I CMGCKG 2 cut(s) 101, 767
MspR9I CCNGG 4 cut(s) 407, 521, 710, 779
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 862
MvaI CCWGG 4 cut(s) 407, 521, 710, 779
MwoI GCNNNNNNNGC 4 cut(s) 53, 98, 587, 1089
NdeII GATC 6 cut(s) 184, 534, 634, 874, 1032, 1038
NlaIII CATG 6 cut(s) 496, 506, 566, 775, 795, 1045
NlaIV GGNNCC 1 cut(s) 340
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 4 cut(s) 36, 80, 212, 659
NspI RCATGY 1 cut(s) 795
OliI CACNNNNGTG 1 cut(s) 911
PagI TCATGA 1 cut(s) 492
PciI ACATGT 1 cut(s) 791
PcsI WCGNNNNNNNCGW 1 cut(s) 832
PctI GAATGC 1 cut(s) 862
PfeI GAWTC 1 cut(s) 362
PfoI TCCNGGA 1 cut(s) 519
PkrI GCNGC 5 cut(s) 46, 49, 249, 766, 1091
PleI GAGTC 3 cut(s) 22, 144, 495
PpsI GAGTC 3 cut(s) 22, 144, 495
PpuMI RGGWCCY 1 cut(s) 338
PscI ACATGT 1 cut(s) 791
PsiI TTATAA 1 cut(s) 66
Psp124BI GAGCTC 2 cut(s) 502, 966
Psp5II RGGWCCY 1 cut(s) 338
Psp6I CCWGG 4 cut(s) 405, 519, 708, 777
PspEI GGTNACC 1 cut(s) 80
PspGI CCWGG 4 cut(s) 405, 519, 708, 777
PspN4I GGNNCC 1 cut(s) 340
PspPI GGNCC 3 cut(s) 338, 452, 517
PspPPI RGGWCCY 1 cut(s) 338
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 225
PsuI RGATCY 2 cut(s) 534, 634
PvuII CAGCTG 2 cut(s) 101, 767
RsaI GTAC 3 cut(s) 681, 808, 902
RsaNI GTAC 3 cut(s) 680, 807, 901
RseI CAYNNNNRTG 1 cut(s) 911
SacI GAGCTC 2 cut(s) 502, 966
SaqAI TTAA 4 cut(s) 176, 897, 989, 1117
SatI GCNGC 5 cut(s) 45, 48, 248, 765, 1090
Sau3AI GATC 6 cut(s) 184, 534, 634, 874, 1032, 1038
Sau96I GGNCC 3 cut(s) 338, 452, 517
ScaI AGTACT 1 cut(s) 902
SchI GAGTC 3 cut(s) 23, 145, 496
ScrFI CCNGG 4 cut(s) 407, 521, 710, 779
SduI GDGCHC 2 cut(s) 502, 966
SexAI ACCWGGT 1 cut(s) 405
SfaNI GCATC 3 cut(s) 477, 514, 1098
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 3 cut(s) 338, 452, 517
SmiMI CAYNNNNRTG 1 cut(s) 911
SmlI CTYRAG 3 cut(s) 152, 463, 684
SmoI CTYRAG 3 cut(s) 152, 463, 684
Sse9I AATT 8 cut(s) 205, 264, 366, 383, 690, 743, 1026, 1095
SsiI CCGC 1 cut(s) 47
SspMI CTAG 3 cut(s) 582, 611, 638
SstI GAGCTC 2 cut(s) 502, 966
StyD4I CCNGG 4 cut(s) 405, 519, 708, 777
StyI CCWWGG 1 cut(s) 891
TaaI ACNGT 3 cut(s) 258, 700, 836
TaiI ACGT 1 cut(s) 981
TasI AATT 8 cut(s) 205, 264, 366, 383, 690, 743, 1026, 1095
TatI WGTACW 2 cut(s) 806, 900
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 1 cut(s) 362
Tru1I TTAA 4 cut(s) 176, 897, 989, 1117
Tru9I TTAA 4 cut(s) 176, 897, 989, 1117
TscAI CASTG 3 cut(s) 199, 217, 421
TseFI GTSAC 4 cut(s) 36, 80, 212, 659
TseI GCWGC 4 cut(s) 44, 247, 764, 1089
Tsp45I GTSAC 4 cut(s) 36, 80, 212, 659
TspDTI ATGAA 4 cut(s) 17, 582, 686, 724
TspRI CASTG 3 cut(s) 199, 217, 421
VpaK11BI GGWCC 3 cut(s) 338, 452, 517
XapI RAATTY 3 cut(s) 383, 690, 1026
XceI RCATGY 1 cut(s) 795
XspI CTAG 3 cut(s) 582, 611, 638
ZraI GACGTC 1 cut(s) 979
ZrmI AGTACT 1 cut(s) 902
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.