Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011496

Physical Location & Seq

Genomic Coordinates Forward (+)

16085635 .. 16087543

Gene Structure 1909 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G40250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1131 bp

ATGTCTTGGGTTCGATTCACAATTGAGCAAAAAGATGGGAACTTTGCGTACCCACCACCGTTTTACAAAGACCCAATTCTTTCTCCGCCGTCTCCTCCACCACCGTCGTCCGGTAACAGAATCAGTCCGGCGGTTTTGTTTGTAATTGTGATTCTAGCTGTCTTGTTCTTCATTTCTGGGCTTCTTCATTTGCTCGTCAGGTTTCTTATTAAGCATCCTTCTGCTACTGCTTCTTCAAGGTCTAATAGATTCCCAGAGATCTCAACTAGTGATGCTCTTCAAAGACAGCTTCAACAGCTTTTTCATTTGAATGATTCTGGTCTTGATCAAGCTTTTATCGATGCTTTACCTGTTTTTCACTACAAGGAGATTGTTGGTAGTGCTGGTGGTGGTGGTGGTAATGGTGCTGCTCAAGAGCCGTTTGATTGCGCGGTTTGTCTCTGTGAGTTCTCTGAGAAAGATAAGCTGAGGTTGTTGCCAATGTGTAGCCATGCTTTTCATTTGAACTGTATTGATACTTGGCTTCAGTCAAATTCGACTTGTCCTCTTTGTCGTGGTACTCTCTTTTCCCCTGGTTTCTCAATGGAGAACCCGATGTTTGATTTCGATGATATAAGAGAAGACGAAGAGGGTGTGACCGAGAATGGAAGTCAGAAAACTATGGAGATTCAAGAAATCGTTGTGGAGAAAGGTGTTTTACCGGTTAGATTGGGGAAATTTAAGAGGCTTGACAATGTTGGTAATGGCCAAGGTCAGGATGTTGTTGCCGGAGGAGAGACTAGTAGTAGTAATTTGGATGCAAGGAGATGTTTCTCAATGGGTTCGTATCAGTATATACTCGGTAACTCGGAACTTAAAGTCCCGTTTGCCAACGACAGACTGCCGCGTTTGAAACCTCAGGACAAAGAATCTGAGCAGACAGGGAATTCATCATCAGAAGACAATAAAAAGATCAATACTGTGGCTAAAGGAGAGAGCTTTTCGGTTTCGAAGATTTGGTTGTGGCCTAAGAAAGATAAGTTCTCTTCAGATGCTCAAAGAAGGTTGCCTTCTTCATCACTTAACGTTGATGATCTTCCGAAACTTCCATGGATGGAAGAACATAAGAAGCTGGAGAACGACGGAAGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

376

Amino Acids

41.79

Weight (kDa)

5.74

Isoelectric Point (pI)

57.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	139 - 185	1.1e-10	RING-H2 zinc finger domain
zf-ANAPC11	PF12861	139 - 187	3.6e-06	Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2	PF13639	142 - 185	2.6e-14	Ring finger domain
zf-RING_11	PF17123	142 - 171	6.9e-10	RING-like zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011496)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40250
fragaria_vesca	FvH4_6g50460
malus_domestica	MD09G1036200.v1.1 MD17G1037800.v1.1
prunus_persica	Prupe.3G281500_v2.0.a1 Prupe.3G281500_v2.0.a1 Prupe.3G281500_v2.0.a1 Prupe.3G281500_v2.0.a1 Prupe.3G281500_v2.0.a1
pyrus_communis	pycom17g03400
rosa_chinensis	RchiOBHm_Chr2g0170871
rosa_laevigata	RLG00000021988
rosa_multiflora	Rmu_sc0009938.1_g000005
rosa_roxburghii	Rroxscaffold_2G00081000
rosa_rugosa	Rorug02G0551900
rosa_samantha	Rh2AG624700 Rh2BG635300 Rh2CG604100 Rh2DG646900
rosa_wichuraiana	Rw2G051750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	431, 886
AciI	CCGC	4 cut(s)	86, 131, 431, 884
AclI	AACGTT	1 cut(s)	1065
AcoI	YGGCCR	1 cut(s)	745
AcsI	RAATTY	3 cut(s)	532, 716, 925
AcuI	CTGAAG	2 cut(s)	509, 1011
AfaI	GTAC	2 cut(s)	50, 559
AfiI	CCNNNNNNNGG	2 cut(s)	110, 754
AgeI	ACCGGT	1 cut(s)	700
AgsI	TTSAA	7 cut(s)	237, 281, 293, 310, 505, 671, 892
AhlI	ACTAGT	2 cut(s)	266, 779
AjnI	CCWGG	1 cut(s)	571
AloI	GAACNNNNNNTCC	2 cut(s)	843, 875
AluBI	AGCT	7 cut(s)	158, 289, 298, 332, 466, 978, 1111
AluI	AGCT	7 cut(s)	158, 289, 298, 332, 466, 978, 1111
Alw26I	GTCTC	3 cut(s)	96, 443, 770
AoxI	GGCC	2 cut(s)	745, 1004
ApeKI	GCWGC	1 cut(s)	407
ApoI	RAATTY	3 cut(s)	532, 716, 925
AsiGI	ACCGGT	1 cut(s)	700
AspLEI	GCGC	1 cut(s)	431
AsuII	TTCGAA	1 cut(s)	989
AxyI	CCTNAGG	1 cut(s)	897
BalI	TGGCCA	1 cut(s)	747
BbsI	GAAGAC	2 cut(s)	627, 945
BbvCI	CCTCAGC	1 cut(s)	467
BbvI	GCAGC	1 cut(s)	394
BccI	CCATC	2 cut(s)	29, 1087
BceAI	ACGGC	2 cut(s)	73, 403
BciT130I	CCWGG	1 cut(s)	573
BclI	TGATCA	1 cut(s)	325
BcoDI	GTCTC	3 cut(s)	96, 443, 770
BcuI	ACTAGT	2 cut(s)	266, 779
BfaI	CTAG	3 cut(s)	155, 267, 780
BglII	AGATCT	1 cut(s)	258
BisI	GCNGC	2 cut(s)	408, 884
BlsI	GCNGC	2 cut(s)	409, 885
Bme1390I	CCNGG	1 cut(s)	573
BmrFI	CCNGG	1 cut(s)	573
BmsI	GCATC	5 cut(s)	223, 262, 331, 787, 1021
BpiI	GAAGAC	2 cut(s)	627, 945
Bpu10I	CCTNAGC	1 cut(s)	467
Bpu14I	TTCGAA	1 cut(s)	989
BpuEI	CTTGAG	1 cut(s)	396
Bsa29I	ATCGAT	1 cut(s)	339
BsaJI	CCNNGG	3 cut(s)	571, 748, 1088
BsaWI	WCCGGW	2 cut(s)	110, 700
Bsc4I	CCNNNNNNNGG	2 cut(s)	110, 754
Bse118I	RCCGGY	1 cut(s)	700
Bse21I	CCTNAGG	1 cut(s)	897
BseBI	CCWGG	1 cut(s)	573
BseCI	ATCGAT	1 cut(s)	339
BseDI	CCNNGG	3 cut(s)	571, 748, 1088
BseGI	GGATG	4 cut(s)	214, 763, 802, 1098
BseLI	CCNNNNNNNGG	2 cut(s)	110, 754
BseMII	CTCAG	4 cut(s)	444, 458, 903, 911
BseRI	GAGGAG	2 cut(s)	84, 786
BseXI	GCAGC	1 cut(s)	394
Bsh1236I	CGCG	2 cut(s)	431, 886
BshFI	GGCC	2 cut(s)	747, 1006
BshTI	ACCGGT	1 cut(s)	700
BshVI	ATCGAT	1 cut(s)	339
BsiSI	CCGG	4 cut(s)	111, 128, 701, 768
BslFI	GGGAC	1 cut(s)	845
BslI	CCNNNNNNNGG	2 cut(s)	110, 754
BsmAI	GTCTC	3 cut(s)	96, 443, 770
BsmBI	CGTCTC	1 cut(s)	96
BsmFI	GGGAC	1 cut(s)	845
BsnI	GGCC	2 cut(s)	747, 1006
Bsp119I	TTCGAA	1 cut(s)	989
Bsp143I	GATC	4 cut(s)	258, 325, 951, 1072
Bsp19I	CCATGG	1 cut(s)	1088
BspACI	CCGC	4 cut(s)	86, 131, 431, 884
BspANI	GGCC	2 cut(s)	747, 1006
BspCNI	CTCAG	4 cut(s)	445, 459, 904, 910
BspDI	ATCGAT	1 cut(s)	339
BspFNI	CGCG	2 cut(s)	431, 886
BspQI	GCTCTTC	1 cut(s)	282
BspT104I	TTCGAA	1 cut(s)	989
BsrFI	RCCGGY	1 cut(s)	700
BssAI	RCCGGY	1 cut(s)	700
BssECI	CCNNGG	3 cut(s)	571, 748, 1088
BssMI	GATC	4 cut(s)	258, 325, 951, 1072
BssT1I	CCWWGG	2 cut(s)	748, 1088
Bst2UI	CCWGG	1 cut(s)	573
Bst4CI	ACNGT	4 cut(s)	60, 105, 509, 961
Bst6I	CTCTTC	3 cut(s)	282, 621, 1030
BstBI	TTCGAA	1 cut(s)	989
BstDEI	CTNAG	5 cut(s)	453, 467, 897, 912, 1008
BstDSI	CCRYGG	1 cut(s)	1088
BstF5I	GGATG	4 cut(s)	214, 763, 802, 1098
BstFNI	CGCG	2 cut(s)	431, 886
BstHHI	GCGC	1 cut(s)	431
BstKTI	GATC	4 cut(s)	261, 328, 954, 1075
BstMAI	GTCTC	3 cut(s)	96, 443, 770
BstMBI	GATC	4 cut(s)	258, 325, 951, 1072
BstMWI	GCNNNNNNNGC	1 cut(s)	295
BstNI	CCWGG	1 cut(s)	573
BstSCI	CCNGG	1 cut(s)	571
BstUI	CGCG	2 cut(s)	431, 886
BstV1I	GCAGC	1 cut(s)	394
BstV2I	GAAGAC	2 cut(s)	627, 945
BstX2I	RGATCY	1 cut(s)	258
BstYI	RGATCY	1 cut(s)	258
Bsu15I	ATCGAT	1 cut(s)	339
Bsu36I	CCTNAGG	1 cut(s)	897
BsuRI	GGCC	2 cut(s)	747, 1006
BsuTUI	ATCGAT	1 cut(s)	339
BtgI	CCRYGG	1 cut(s)	1088
BtsCI	GGATG	4 cut(s)	214, 763, 802, 1098
CfoI	GCGC	1 cut(s)	431
Cfr10I	RCCGGY	1 cut(s)	700
ClaI	ATCGAT	1 cut(s)	339
Csp6I	GTAC	2 cut(s)	49, 558
CspAI	ACCGGT	1 cut(s)	700
CviAII	CATG	2 cut(s)	491, 1089
CviQI	GTAC	2 cut(s)	49, 558
DdeI	CTNAG	5 cut(s)	453, 467, 897, 912, 1008
DpnI	GATC	4 cut(s)	260, 327, 953, 1074
DpnII	GATC	4 cut(s)	258, 325, 951, 1072
EaeI	YGGCCR	1 cut(s)	745
Eam1104I	CTCTTC	3 cut(s)	282, 621, 1030
EarI	CTCTTC	3 cut(s)	282, 621, 1030
EciI	GGCGGA	1 cut(s)	75
Eco130I	CCWWGG	2 cut(s)	748, 1088
Eco57I	CTGAAG	2 cut(s)	509, 1011
Eco81I	CCTNAGG	1 cut(s)	897
EcoRI	GAATTC	1 cut(s)	925
EcoRII	CCWGG	1 cut(s)	571
EcoT14I	CCWWGG	2 cut(s)	748, 1088
ErhI	CCWWGG	2 cut(s)	748, 1088
Esp3I	CGTCTC	1 cut(s)	96
FaeI	CATG	2 cut(s)	494, 1092
FaiI	YATR	7 cut(s)	492, 614, 662, 834, 836, 1090, 1104
FalI	AAGNNNNNCTT	2 cut(s)	1033, 1065
FaqI	GGGAC	1 cut(s)	845
FatI	CATG	2 cut(s)	490, 1088
FbaI	TGATCA	1 cut(s)	325
Fnu4HI	GCNGC	2 cut(s)	408, 884
FokI	GGATG	4 cut(s)	201, 770, 809, 1105
Fsp4HI	GCNGC	2 cut(s)	408, 884
FspBI	CTAG	3 cut(s)	155, 267, 780
GlaI	GCGC	1 cut(s)	430
GluI	GCNGC	2 cut(s)	408, 884
HaeIII	GGCC	2 cut(s)	747, 1006
HapII	CCGG	4 cut(s)	111, 128, 701, 768
HhaI	GCGC	1 cut(s)	431
Hin1II	CATG	2 cut(s)	494, 1092
Hin6I	GCGC	1 cut(s)	429
HinP1I	GCGC	1 cut(s)	429
HindIII	AAGCTT	1 cut(s)	330
HinfI	GANTC	7 cut(s)	15, 120, 151, 249, 314, 667, 908
HpaII	CCGG	4 cut(s)	111, 128, 701, 768
Hpy188I	TCNGA	7 cut(s)	454, 654, 850, 913, 937, 1030, 1080
Hpy188III	TCNNGA	5 cut(s)	323, 413, 671, 755, 899
Hpy99I	CGWCG	2 cut(s)	109, 1124
HpyAV	CCTTC	4 cut(s)	228, 1035, 1059, 1119
HpyCH4III	ACNGT	4 cut(s)	60, 105, 509, 961
HpyCH4IV	ACGT	1 cut(s)	1065
HpyCH4V	TGCA	1 cut(s)	800
HpyF10VI	GCNNNNNNNGC	1 cut(s)	295
HpyF3I	CTNAG	5 cut(s)	453, 467, 897, 912, 1008
HpySE526I	ACGT	1 cut(s)	1065
Hsp92II	CATG	2 cut(s)	494, 1092
HspAI	GCGC	1 cut(s)	429
Ksp22I	TGATCA	1 cut(s)	325
Kzo9I	GATC	4 cut(s)	258, 325, 951, 1072
LguI	GCTCTTC	1 cut(s)	282
Lsp1109I	GCAGC	1 cut(s)	394
LweI	GCATC	5 cut(s)	223, 262, 331, 787, 1021
MaeI	CTAG	3 cut(s)	155, 267, 780
MaeII	ACGT	1 cut(s)	1065
MaeIII	GTNAC	3 cut(s)	113, 634, 842
MalI	GATC	4 cut(s)	260, 327, 953, 1074
MboI	GATC	4 cut(s)	258, 325, 951, 1072
MfeI	CAATTG	1 cut(s)	21
MflI	RGATCY	1 cut(s)	258
MlsI	TGGCCA	1 cut(s)	747
MluCI	AATT	7 cut(s)	21, 75, 144, 532, 716, 790, 925
MluNI	TGGCCA	1 cut(s)	747
MnlI	CCTC	7 cut(s)	105, 462, 555, 622, 717, 764, 906
Mox20I	TGGCCA	1 cut(s)	747
MscI	TGGCCA	1 cut(s)	747
MseI	TTAA	4 cut(s)	210, 720, 855, 1062
MslI	CAYNNNNRTG	1 cut(s)	309
Msp20I	TGGCCA	1 cut(s)	747
MspI	CCGG	4 cut(s)	111, 128, 701, 768
MspR9I	CCNGG	1 cut(s)	573
MunI	CAATTG	1 cut(s)	21
MvaI	CCWGG	1 cut(s)	573
MvnI	CGCG	2 cut(s)	431, 886
MwoI	GCNNNNNNNGC	1 cut(s)	295
NcoI	CCATGG	1 cut(s)	1088
NdeII	GATC	4 cut(s)	258, 325, 951, 1072
NlaIII	CATG	2 cut(s)	494, 1092
NmuCI	GTSAC	1 cut(s)	634
NspV	TTCGAA	1 cut(s)	989
PciSI	GCTCTTC	1 cut(s)	282
PfeI	GAWTC	7 cut(s)	15, 120, 151, 249, 314, 667, 908
PinAI	ACCGGT	1 cut(s)	700
PkrI	GCNGC	2 cut(s)	409, 885
Psp1406I	AACGTT	1 cut(s)	1065
Psp6I	CCWGG	1 cut(s)	571
PspGI	CCWGG	1 cut(s)	571
PsrI	GAACNNNNNNTAC	2 cut(s)	32, 64
PsuI	RGATCY	1 cut(s)	258
RsaI	GTAC	2 cut(s)	50, 559
RsaNI	GTAC	2 cut(s)	49, 558
RseI	CAYNNNNRTG	1 cut(s)	309
SapI	GCTCTTC	1 cut(s)	282
SaqAI	TTAA	4 cut(s)	210, 720, 855, 1062
SatI	GCNGC	2 cut(s)	408, 884
Sau3AI	GATC	4 cut(s)	258, 325, 951, 1072
ScrFI	CCNGG	1 cut(s)	573
SfaNI	GCATC	5 cut(s)	223, 262, 331, 787, 1021
SfuI	TTCGAA	1 cut(s)	989
SmiMI	CAYNNNNRTG	1 cut(s)	309
SmlI	CTYRAG	1 cut(s)	411
SmoI	CTYRAG	1 cut(s)	411
SpeI	ACTAGT	2 cut(s)	266, 779
Sse9I	AATT	7 cut(s)	21, 75, 144, 532, 716, 790, 925
SsiI	CCGC	4 cut(s)	86, 131, 431, 884
SspMI	CTAG	3 cut(s)	155, 267, 780
StyD4I	CCNGG	1 cut(s)	571
StyI	CCWWGG	2 cut(s)	748, 1088
TaaI	ACNGT	4 cut(s)	60, 105, 509, 961
TaiI	ACGT	1 cut(s)	1068
TaqI	TCGA	5 cut(s)	13, 339, 536, 606, 989
TaqII	GACCGA	1 cut(s)	653
TasI	AATT	7 cut(s)	21, 75, 144, 532, 716, 790, 925
TauI	GCSGC	1 cut(s)	886
TfiI	GAWTC	7 cut(s)	15, 120, 151, 249, 314, 667, 908
Tru1I	TTAA	4 cut(s)	210, 720, 855, 1062
Tru9I	TTAA	4 cut(s)	210, 720, 855, 1062
TseFI	GTSAC	1 cut(s)	634
TseI	GCWGC	1 cut(s)	407
Tsp45I	GTSAC	1 cut(s)	634
TspDTI	ATGAA	6 cut(s)	160, 176, 293, 488, 918, 1044
XapI	RAATTY	3 cut(s)	532, 716, 925
XspI	CTAG	3 cut(s)	155, 267, 780

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.