contain most of the conserved domains of hsU2AF35, including the psiRRM, one RS domain, two zinc fingers, and the two regions for interacting with U2AF large subunit. Both proteins lack the stretch of glycines present in human U2AF35. The sequences are overall 83 identical, and each Arabidopsis homolog shows approximately 70 similarity to hsU2AF35. U2AF(35) homologs were also identified from maize, rice and other plants with large-scale EST projects. Both genes are expressed in all major tissues, with atU2AF(35)a expressed at a higher level than atU2AF(35)b in most tissues. The expression patterns were different in roots atU2AF(35)b expressed strongly in whole young roots and root tips and atU2AF(35)a limited to root vascular regions
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G42820 AT5G42820 |
| fragaria_vesca | FvH4_5g26450 FvH4_5g26450 |
| pyrus_communis | pycom06g00210 |
| rosa_chinensis | RchiOBHm_Chr7g0217361 |
| rosa_laevigata | RLG00000002486 |
| rosa_multiflora | Rmu_sc0011824.1_g000010 Rmu_sc0038917.1_g000002 |
| rosa_roxburghii | Rroxscaffold_3G00242300 |
| rosa_rugosa | Rorug07G0168300 Rorug07G0168400 |
| rosa_samantha | Rh7AG308100 Rh7BG298800 Rh7DG307200 |
| rosa_wichuraiana | Rw7G026120 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 637 |
| AclWI | GGATC | 4 cut(s) | 182, 195, 214, 366 |
| AfaI | GTAC | 3 cut(s) | 31, 155, 500 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 235 |
| AflIII | ACRYGT | 1 cut(s) | 150 |
| AgeI | ACCGGT | 1 cut(s) | 93 |
| AgsI | TTSAA | 1 cut(s) | 21 |
| AjnI | CCWGG | 1 cut(s) | 178 |
| AluBI | AGCT | 5 cut(s) | 17, 368, 455, 483, 538 |
| AluI | AGCT | 5 cut(s) | 17, 368, 455, 483, 538 |
| Alw21I | GWGCWC | 1 cut(s) | 101 |
| Alw26I | GTCTC | 3 cut(s) | 154, 296, 736 |
| AlwI | GGATC | 4 cut(s) | 182, 195, 214, 366 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 371 |
| Ama87I | CYCGRG | 1 cut(s) | 211 |
| ApeKI | GCWGC | 3 cut(s) | 365, 368, 371 |
| AsiGI | ACCGGT | 1 cut(s) | 93 |
| AspS9I | GGNCC | 1 cut(s) | 664 |
| AsuHPI | GGTGA | 2 cut(s) | 101, 290 |
| AsuII | TTCGAA | 1 cut(s) | 258 |
| AvaI | CYCGRG | 1 cut(s) | 211 |
| AvaII | GGWCC | 1 cut(s) | 664 |
| AxyI | CCTNAGG | 1 cut(s) | 192 |
| BamHI | GGATCC | 1 cut(s) | 187 |
| Bbv12I | GWGCWC | 1 cut(s) | 101 |
| BbvI | GCAGC | 3 cut(s) | 355, 358, 377 |
| BccI | CCATC | 2 cut(s) | 131, 719 |
| BceAI | ACGGC | 2 cut(s) | 119, 187 |
| BciT130I | CCWGG | 1 cut(s) | 180 |
| BclI | TGATCA | 1 cut(s) | 316 |
| BcoDI | GTCTC | 3 cut(s) | 154, 296, 736 |
| BisI | GCNGC | 3 cut(s) | 366, 369, 372 |
| BlsI | GCNGC | 3 cut(s) | 367, 370, 373 |
| Bme1390I | CCNGG | 1 cut(s) | 180 |
| Bme18I | GGWCC | 1 cut(s) | 664 |
| BmeT110I | CYCGRG | 1 cut(s) | 211 |
| BmgT120I | GGNCC | 1 cut(s) | 664 |
| BmiI | GGNNCC | 3 cut(s) | 189, 665, 827 |
| BmrFI | CCNGG | 1 cut(s) | 180 |
| BpmI | CTGGAG | 1 cut(s) | 162 |
| Bpu10I | CCTNAGC | 1 cut(s) | 539 |
| Bpu14I | TTCGAA | 1 cut(s) | 258 |
| BsaI | GGTCTC | 1 cut(s) | 154 |
| BsaJI | CCNNGG | 1 cut(s) | 490 |
| BsaWI | WCCGGW | 2 cut(s) | 93, 404 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 550, 580 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 235 |
| Bse118I | RCCGGY | 2 cut(s) | 93, 672 |
| Bse21I | CCTNAGG | 1 cut(s) | 192 |
| Bse3DI | GCAATG | 2 cut(s) | 417, 827 |
| BseBI | CCWGG | 1 cut(s) | 180 |
| BseDI | CCNNGG | 1 cut(s) | 490 |
| BseGI | GGATG | 1 cut(s) | 730 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 235 |
| BseMI | GCAATG | 2 cut(s) | 417, 827 |
| BseMII | CTCAG | 1 cut(s) | 206 |
| BseRI | GAGGAG | 3 cut(s) | 131, 368, 754 |
| BseXI | GCAGC | 3 cut(s) | 355, 358, 377 |
| Bsh1236I | CGCG | 1 cut(s) | 637 |
| Bsh1285I | CGRYCG | 1 cut(s) | 409 |
| BshTI | ACCGGT | 1 cut(s) | 93 |
| BsiEI | CGRYCG | 1 cut(s) | 409 |
| BsiHKAI | GWGCWC | 1 cut(s) | 101 |
| BsiHKCI | CYCGRG | 1 cut(s) | 211 |
| BsiSI | CCGG | 3 cut(s) | 94, 405, 673 |
| BslFI | GGGAC | 4 cut(s) | 102, 210, 668, 677 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 235 |
| BsmAI | GTCTC | 3 cut(s) | 154, 296, 736 |
| BsmFI | GGGAC | 4 cut(s) | 102, 210, 668, 677 |
| BsmI | GAATGC | 1 cut(s) | 631 |
| Bso31I | GGTCTC | 1 cut(s) | 154 |
| BsoBI | CYCGRG | 1 cut(s) | 211 |
| Bsp119I | TTCGAA | 1 cut(s) | 258 |
| Bsp1286I | GDGCHC | 1 cut(s) | 101 |
| Bsp143I | GATC | 6 cut(s) | 187, 219, 316, 358, 704, 718 |
| Bsp68I | TCGCGA | 1 cut(s) | 637 |
| BspCNI | CTCAG | 1 cut(s) | 205 |
| BspFNI | CGCG | 1 cut(s) | 637 |
| BspLI | GGNNCC | 3 cut(s) | 189, 665, 827 |
| BspPI | GGATC | 4 cut(s) | 182, 195, 214, 366 |
| BspT104I | TTCGAA | 1 cut(s) | 258 |
| BspTNI | GGTCTC | 1 cut(s) | 154 |
| BsrDI | GCAATG | 2 cut(s) | 417, 827 |
| BsrFI | RCCGGY | 2 cut(s) | 93, 672 |
| BssAI | RCCGGY | 2 cut(s) | 93, 672 |
| BssECI | CCNNGG | 1 cut(s) | 490 |
| BssMI | GATC | 6 cut(s) | 187, 219, 316, 358, 704, 718 |
| Bst2UI | CCWGG | 1 cut(s) | 180 |
| Bst4CI | ACNGT | 6 cut(s) | 89, 116, 491, 503, 578, 668 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 371, 385 |
| BstBI | TTCGAA | 1 cut(s) | 258 |
| BstC8I | GCNNGC | 1 cut(s) | 381 |
| BstDEI | CTNAG | 2 cut(s) | 192, 539 |
| BstDSI | CCRYGG | 1 cut(s) | 490 |
| BstEII | GGTNACC | 1 cut(s) | 89 |
| BstF5I | GGATG | 1 cut(s) | 730 |
| BstFNI | CGCG | 1 cut(s) | 637 |
| BstKTI | GATC | 6 cut(s) | 190, 222, 319, 361, 707, 721 |
| BstMAI | GTCTC | 3 cut(s) | 154, 296, 736 |
| BstMBI | GATC | 6 cut(s) | 187, 219, 316, 358, 704, 718 |
| BstMCI | CGRYCG | 1 cut(s) | 409 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 371, 380, 385, 520 |
| BstNI | CCWGG | 1 cut(s) | 180 |
| BstNSI | RCATGY | 2 cut(s) | 154, 518 |
| BstPI | GGTNACC | 1 cut(s) | 89 |
| BstSCI | CCNGG | 1 cut(s) | 178 |
| BstUI | CGCG | 1 cut(s) | 637 |
| BstV1I | GCAGC | 3 cut(s) | 355, 358, 377 |
| BstX2I | RGATCY | 2 cut(s) | 187, 219 |
| BstYI | RGATCY | 2 cut(s) | 187, 219 |
| Bsu36I | CCTNAGG | 1 cut(s) | 192 |
| BtgI | CCRYGG | 1 cut(s) | 490 |
| BtsCI | GGATG | 1 cut(s) | 730 |
| BtsI | GCAGTG | 1 cut(s) | 703 |
| BtsIMutI | CAGTG | 1 cut(s) | 703 |
| BtuMI | TCGCGA | 1 cut(s) | 637 |
| Cac8I | GCNNGC | 1 cut(s) | 381 |
| CaiI | CAGNNNCTG | 1 cut(s) | 371 |
| Cfr10I | RCCGGY | 2 cut(s) | 93, 672 |
| Cfr13I | GGNCC | 1 cut(s) | 664 |
| CseI | GACGC | 1 cut(s) | 142 |
| Csp6I | GTAC | 3 cut(s) | 30, 154, 499 |
| CspAI | ACCGGT | 1 cut(s) | 93 |
| CviAII | CATG | 7 cut(s) | 151, 169, 511, 515, 677, 716, 746 |
| CviQI | GTAC | 3 cut(s) | 30, 154, 499 |
| DdeI | CTNAG | 2 cut(s) | 192, 539 |
| DpnI | GATC | 6 cut(s) | 189, 221, 318, 360, 706, 720 |
| DpnII | GATC | 6 cut(s) | 187, 219, 316, 358, 704, 718 |
| Eco31I | GGTCTC | 1 cut(s) | 154 |
| Eco47I | GGWCC | 1 cut(s) | 664 |
| Eco81I | CCTNAGG | 1 cut(s) | 192 |
| Eco88I | CYCGRG | 1 cut(s) | 211 |
| Eco91I | GGTNACC | 1 cut(s) | 89 |
| EcoO65I | GGTNACC | 1 cut(s) | 89 |
| EcoRII | CCWGG | 1 cut(s) | 178 |
| EcoT22I | ATGCAT | 1 cut(s) | 516 |
| FaeI | CATG | 7 cut(s) | 154, 172, 514, 518, 680, 719, 749 |
| FaqI | GGGAC | 4 cut(s) | 102, 210, 668, 677 |
| FatI | CATG | 7 cut(s) | 150, 168, 510, 514, 676, 715, 745 |
| FauNDI | CATATG | 1 cut(s) | 321 |
| FbaI | TGATCA | 1 cut(s) | 316 |
| Fnu4HI | GCNGC | 3 cut(s) | 366, 369, 372 |
| FokI | GGATG | 1 cut(s) | 737 |
| Fsp4HI | GCNGC | 3 cut(s) | 366, 369, 372 |
| GluI | GCNGC | 3 cut(s) | 366, 369, 372 |
| GsuI | CTGGAG | 1 cut(s) | 162 |
| HapII | CCGG | 3 cut(s) | 94, 405, 673 |
| HgaI | GACGC | 1 cut(s) | 142 |
| Hin1II | CATG | 7 cut(s) | 154, 172, 514, 518, 680, 719, 749 |
| HinfI | GANTC | 1 cut(s) | 289 |
| HpaII | CCGG | 3 cut(s) | 94, 405, 673 |
| HphI | GGTGA | 2 cut(s) | 101, 290 |
| Hpy188I | TCNGA | 1 cut(s) | 709 |
| Hpy188III | TCNNGA | 3 cut(s) | 145, 636, 722 |
| Hpy99I | CGWCG | 4 cut(s) | 136, 570, 582, 621 |
| HpyAV | CCTTC | 2 cut(s) | 729, 782 |
| HpyCH4III | ACNGT | 6 cut(s) | 89, 116, 491, 503, 578, 668 |
| HpyCH4IV | ACGT | 3 cut(s) | 619, 657, 835 |
| HpyCH4V | TGCA | 3 cut(s) | 379, 388, 514 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 371, 380, 385, 520 |
| HpyF3I | CTNAG | 2 cut(s) | 192, 539 |
| HpySE526I | ACGT | 3 cut(s) | 619, 657, 835 |
| Hsp92II | CATG | 7 cut(s) | 154, 172, 514, 518, 680, 719, 749 |
| Ksp22I | TGATCA | 1 cut(s) | 316 |
| Kzo9I | GATC | 6 cut(s) | 187, 219, 316, 358, 704, 718 |
| LmnI | GCTCC | 3 cut(s) | 535, 693, 762 |
| Lsp1109I | GCAGC | 3 cut(s) | 355, 358, 377 |
| MaeII | ACGT | 3 cut(s) | 619, 657, 835 |
| MaeIII | GTNAC | 6 cut(s) | 83, 89, 302, 436, 485, 668 |
| MalI | GATC | 6 cut(s) | 189, 221, 318, 360, 706, 720 |
| MboI | GATC | 6 cut(s) | 187, 219, 316, 358, 704, 718 |
| MboII | GAAGA | 3 cut(s) | 260, 272, 485 |
| MflI | RGATCY | 2 cut(s) | 187, 219 |
| MhlI | GDGCHC | 1 cut(s) | 101 |
| MluCI | AATT | 4 cut(s) | 49, 505, 548, 813 |
| MlyI | GAGTC | 1 cut(s) | 298 |
| Mph1103I | ATGCAT | 1 cut(s) | 516 |
| MseI | TTAA | 3 cut(s) | 66, 348, 850 |
| MslI | CAYNNNNRTG | 2 cut(s) | 515, 681 |
| MspA1I | CMGCKG | 2 cut(s) | 368, 483 |
| MspI | CCGG | 3 cut(s) | 94, 405, 673 |
| MspR9I | CCNGG | 1 cut(s) | 180 |
| Mva1269I | GAATGC | 1 cut(s) | 631 |
| MvaI | CCWGG | 1 cut(s) | 180 |
| MvnI | CGCG | 1 cut(s) | 637 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 371, 380, 385, 520 |
| NdeI | CATATG | 1 cut(s) | 321 |
| NdeII | GATC | 6 cut(s) | 187, 219, 316, 358, 704, 718 |
| NlaIII | CATG | 7 cut(s) | 154, 172, 514, 518, 680, 719, 749 |
| NlaIV | GGNNCC | 3 cut(s) | 189, 665, 827 |
| NmuCI | GTSAC | 5 cut(s) | 83, 89, 302, 436, 668 |
| NruI | TCGCGA | 1 cut(s) | 637 |
| NsiI | ATGCAT | 1 cut(s) | 516 |
| NspI | RCATGY | 2 cut(s) | 154, 518 |
| NspV | TTCGAA | 1 cut(s) | 258 |
| PciI | ACATGT | 1 cut(s) | 150 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 574 |
| PctI | GAATGC | 1 cut(s) | 631 |
| PinAI | ACCGGT | 1 cut(s) | 93 |
| PkrI | GCNGC | 3 cut(s) | 367, 370, 373 |
| PleI | GAGTC | 1 cut(s) | 297 |
| PpsI | GAGTC | 1 cut(s) | 297 |
| PscI | ACATGT | 1 cut(s) | 150 |
| Psp6I | CCWGG | 1 cut(s) | 178 |
| PspEI | GGTNACC | 1 cut(s) | 89 |
| PspGI | CCWGG | 1 cut(s) | 178 |
| PspN4I | GGNNCC | 3 cut(s) | 189, 665, 827 |
| PspPI | GGNCC | 1 cut(s) | 664 |
| PstNI | CAGNNNCTG | 1 cut(s) | 371 |
| PsuI | RGATCY | 2 cut(s) | 187, 219 |
| PvuII | CAGCTG | 2 cut(s) | 368, 483 |
| RruI | TCGCGA | 1 cut(s) | 637 |
| RsaI | GTAC | 3 cut(s) | 31, 155, 500 |
| RsaNI | GTAC | 3 cut(s) | 30, 154, 499 |
| RseI | CAYNNNNRTG | 2 cut(s) | 515, 681 |
| SaqAI | TTAA | 3 cut(s) | 66, 348, 850 |
| SatI | GCNGC | 3 cut(s) | 366, 369, 372 |
| Sau3AI | GATC | 6 cut(s) | 187, 219, 316, 358, 704, 718 |
| Sau96I | GGNCC | 1 cut(s) | 664 |
| SchI | GAGTC | 1 cut(s) | 298 |
| ScrFI | CCNGG | 1 cut(s) | 180 |
| SduI | GDGCHC | 1 cut(s) | 101 |
| SfuI | TTCGAA | 1 cut(s) | 258 |
| SinI | GGWCC | 1 cut(s) | 664 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 515, 681 |
| Sse9I | AATT | 4 cut(s) | 49, 505, 548, 813 |
| StyD4I | CCNGG | 1 cut(s) | 178 |
| TaaI | ACNGT | 6 cut(s) | 89, 116, 491, 503, 578, 668 |
| TaiI | ACGT | 3 cut(s) | 622, 660, 838 |
| TaqI | TCGA | 5 cut(s) | 146, 258, 530, 595, 616 |
| TasI | AATT | 4 cut(s) | 49, 505, 548, 813 |
| Tru1I | TTAA | 3 cut(s) | 66, 348, 850 |
| Tru9I | TTAA | 3 cut(s) | 66, 348, 850 |
| TscAI | CASTG | 1 cut(s) | 703 |
| TseFI | GTSAC | 5 cut(s) | 83, 89, 302, 436, 668 |
| TseI | GCWGC | 3 cut(s) | 365, 368, 371 |
| Tsp45I | GTSAC | 5 cut(s) | 83, 89, 302, 436, 668 |
| TspDTI | ATGAA | 4 cut(s) | 98, 486, 499, 561 |
| TspGWI | ACGGA | 3 cut(s) | 45, 455, 775 |
| TspRI | CASTG | 1 cut(s) | 703 |
| VpaK11BI | GGWCC | 1 cut(s) | 664 |
| XceI | RCATGY | 2 cut(s) | 154, 518 |
| Zsp2I | ATGCAT | 1 cut(s) | 516 |