Rh7DG307200

contain most of the conserved domains of hsU2AF35, including the psiRRM, one RS domain, two zinc fingers, and the two regions for interacting with U2AF large subunit. Both proteins lack the stretch of glycines present in human U2AF35. The sequences are overall 83 identical, and each Arabidopsis homolog shows approximately 70 similarity to hsU2AF35. U2AF(35) homologs were also identified from maize, rice and other plants with large-scale EST projects. Both genes are expressed in all major tissues, with atU2AF(35)a expressed at a higher level than atU2AF(35)b in most tissues. The expression patterns were different in roots atU2AF(35)b expressed strongly in whole young roots and root tips and atU2AF(35)a limited to root vascular regions

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0014926
Physical Location & Seq
Reverse (-)
35862829 .. 35867081
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG307200.1

Sequence Viewer

Length: 843 bp
ATGGCGGAGCACTTGGCTTCGATATTCGGGACCGAGAAGGACCGGGTGAACTGCCCGTTCTATTTCAAGATCGGCGCGTGCCGTCACGGCGACCGGTGCTCCCGTCTCCACAACCGGCCGACGATCAGCCCGACCCTGCTCCTCTCCAACATGTACCAGCGCCCCGACATGATCACCCCCGGCGTCGACCCCCAGGGCCAGCCCATCGACCCCCGCAAGATCCAGGACCATTTCGAGGACTTCTACGAGGACATCTTCGAGGAGCTCGACAAGTTCGGCGAGATCGAGAGCCTCAACGTCTGCGACAATCTCGCCGATCACATGATCGGCAACGTCTACGTCCAGTTCAAGGAGGAGGACCAGGCCGCCGCCGCCCTCCAGGCCTTGCAAGGCAGGTTCTATAACGGCCGCCCGATTATCGCCGACTTCTCGCCCGTGACGGATTTCCGGGAAGCCACGTGTCGCCAGTTCGAGGAGAACAACTGCAATCGAGGCGGCTACTGTAATTTTATGCACGTCAAGCTGATTGGGAGGGAATTGAGGAGGAAGCTGTATGGGAGGTACCGCGGGTATAGGGCTAGCCGGAGCCGGAGCCGGAGTTTGAGCCCGCGGCACCGGAGGGATAGGGATAGGGATTATGATAAACGGGAGCACCGGGACAGAGATTATAGAGGGAATGGGAGGAGAAGCAAGGATAGGTATGACAGGGACAGGGACGGTGGGAGGAGAAGACATAGCAGTCCCAGGCGGAGCAGGAGCCCCGTGGCGGCAGTGAGGGAAGGGAGCGAAGAACGCCGAGCTAGGATTGAGCAGTGGAATCGAGAAAGGGAGGGGAAGCTGTGA

Protein Analysis

280

Amino Acids

33.17

Weight (kDa)

9.52

Isoelectric Point (pI)

66.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCCH PF00642 14 - 38 1.2e-07 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
RRM_1 PF00076 83 - 139 1.5e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G42820 AT5G42820
fragaria_vesca FvH4_5g26450 FvH4_5g26450
pyrus_communis pycom06g00210
rosa_chinensis RchiOBHm_Chr7g0217361
rosa_laevigata RLG00000002486
rosa_multiflora Rmu_sc0011824.1_g000010 Rmu_sc0038917.1_g000002
rosa_roxburghii Rroxscaffold_3G00242300
rosa_rugosa Rorug07G0168300 Rorug07G0168400
rosa_samantha Rh7AG308100 Rh7BG298800 Rh7DG307200
rosa_wichuraiana Rw7G026120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 738
Acc36I ACCTGC 1 cut(s) 384
Acc65I GGTACC 1 cut(s) 561
AccB1I GGYRCC 2 cut(s) 561, 612
AccI GTMKAC 2 cut(s) 186, 336
AccII CGCG 3 cut(s) 77, 567, 610
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 2 cut(s) 116, 406
AcvI CACGTG 1 cut(s) 459
AcyI GRCGYC 1 cut(s) 183
AfaI GTAC 2 cut(s) 155, 563
AfiI CCNNNNNNNGG 4 cut(s) 235, 349, 472, 766
AflIII ACRYGT 2 cut(s) 150, 458
AgeI ACCGGT 1 cut(s) 93
AgsI TTSAA 2 cut(s) 67, 349
AjiI CACGTC 1 cut(s) 517
AjnI CCWGG 5 cut(s) 192, 222, 360, 378, 743
AluBI AGCT 5 cut(s) 265, 523, 550, 800, 838
AluI AGCT 5 cut(s) 265, 523, 550, 800, 838
Alw21I GWGCWC 4 cut(s) 12, 101, 267, 654
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 1 cut(s) 214
AoxI GGCC 5 cut(s) 116, 196, 363, 381, 406
AsiGI ACCGGT 1 cut(s) 93
Asp718I GGTACC 1 cut(s) 561
AspLEI GCGC 2 cut(s) 77, 162
AspS9I GGNCC 5 cut(s) 30, 40, 196, 226, 358
AsuC2I CCSGG 4 cut(s) 44, 180, 449, 656
AsuHPI GGTGA 2 cut(s) 58, 166
AsuNHI GCTAGC 1 cut(s) 578
AvaII GGWCC 4 cut(s) 30, 40, 226, 358
BanI GGYRCC 2 cut(s) 561, 612
BanII GRGCYC 3 cut(s) 267, 608, 761
BbrPI CACGTG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 736
Bbv12I GWGCWC 4 cut(s) 12, 101, 267, 654
BccI CCATC 1 cut(s) 212
BceAI ACGGC 3 cut(s) 66, 103, 421
BcgI CGANNNNNNTGC 2 cut(s) 800, 834
BciT130I CCWGG 5 cut(s) 194, 224, 362, 380, 745
BclI TGATCA 1 cut(s) 171
BcnI CCSGG 4 cut(s) 44, 180, 449, 656
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 2 cut(s) 579, 801
BfoI RGCGCY 1 cut(s) 163
BfuAI ACCTGC 1 cut(s) 384
BglI GCCNNNNNGGC 2 cut(s) 87, 380
BisI GCNGC 7 cut(s) 366, 369, 372, 409, 496, 611, 768
BlsI GCNGC 7 cut(s) 367, 370, 373, 410, 497, 612, 769
Bme1390I CCNGG 9 cut(s) 44, 180, 194, 224, 362, 380, 449, 656, 745
Bme18I GGWCC 4 cut(s) 30, 40, 226, 358
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 5 cut(s) 30, 40, 196, 226, 358
BmiI GGNNCC 6 cut(s) 31, 563, 587, 593, 614, 758
BmrFI CCNGG 9 cut(s) 44, 180, 194, 224, 362, 380, 449, 656, 745
BmtI GCTAGC 1 cut(s) 582
BpiI GAAGAC 1 cut(s) 736
BpmI CTGGAG 1 cut(s) 362
BpuMI CCSGG 4 cut(s) 44, 180, 449, 656
BsaAI YACGTR 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 183
BsaJI CCNNGG 7 cut(s) 178, 192, 193, 565, 608, 743, 762
BsaWI WCCGGW 2 cut(s) 93, 615
BsaXI ACNNNNNCTCC 4 cut(s) 83, 113, 583, 613
Bsc4I CCNNNNNNNGG 4 cut(s) 235, 349, 472, 766
Bse118I RCCGGY 2 cut(s) 93, 114
Bse1I ACTGG 2 cut(s) 343, 466
BseBI CCWGG 5 cut(s) 194, 224, 362, 380, 745
BseDI CCNNGG 7 cut(s) 178, 192, 193, 565, 608, 743, 762
BseLI CCNNNNNNNGG 4 cut(s) 235, 349, 472, 766
BseNI ACTGG 2 cut(s) 343, 466
BseRI GAGGAG 7 cut(s) 131, 275, 368, 488, 556, 697, 739
BseX3I CGGCCG 2 cut(s) 116, 406
Bsh1236I CGCG 3 cut(s) 77, 567, 610
Bsh1285I CGRYCG 3 cut(s) 94, 119, 409
BshFI GGCC 5 cut(s) 118, 198, 365, 383, 408
BshNI GGYRCC 2 cut(s) 561, 612
BshTI ACCGGT 1 cut(s) 93
BsiEI CGRYCG 3 cut(s) 94, 119, 409
BsiHKAI GWGCWC 4 cut(s) 12, 101, 267, 654
BslFI GGGAC 5 cut(s) 43, 671, 722, 726, 728
BslI CCNNNNNNNGG 4 cut(s) 235, 349, 472, 766
BsmAI GTCTC 1 cut(s) 110
BsmBI CGTCTC 1 cut(s) 110
BsmFI GGGAC 5 cut(s) 43, 671, 722, 726, 728
BsnI GGCC 5 cut(s) 118, 198, 365, 383, 408
Bsp1286I GDGCHC 6 cut(s) 12, 101, 267, 608, 654, 761
Bsp143I GATC 7 cut(s) 69, 123, 171, 219, 282, 316, 324
BspANI GGCC 5 cut(s) 118, 198, 365, 383, 408
BspFNI CGCG 3 cut(s) 77, 567, 610
BspLI GGNNCC 6 cut(s) 31, 563, 587, 593, 614, 758
BspMI ACCTGC 1 cut(s) 384
BspOI GCTAGC 1 cut(s) 582
BspPI GGATC 1 cut(s) 214
BspT107I GGYRCC 2 cut(s) 561, 612
BsrFI RCCGGY 2 cut(s) 93, 114
BsrI ACTGG 2 cut(s) 343, 466
BssAI RCCGGY 2 cut(s) 93, 114
BssECI CCNNGG 7 cut(s) 178, 192, 193, 565, 608, 743, 762
BssMI GATC 7 cut(s) 69, 123, 171, 219, 282, 316, 324
BssNI GRCGYC 1 cut(s) 183
Bst2UI CCWGG 5 cut(s) 194, 224, 362, 380, 745
Bst4CI ACNGT 2 cut(s) 503, 719
BstACI GRCGYC 1 cut(s) 183
BstBAI YACGTR 1 cut(s) 459
BstC8I GCNNGC 4 cut(s) 79, 200, 580, 608
BstDSI CCRYGG 3 cut(s) 565, 608, 762
BstFNI CGCG 3 cut(s) 77, 567, 610
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 2 cut(s) 77, 162
BstKTI GATC 7 cut(s) 72, 126, 174, 222, 285, 319, 327
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 7 cut(s) 69, 123, 171, 219, 282, 316, 324
BstMCI CGRYCG 3 cut(s) 94, 119, 409
BstMWI GCNNNNNNNGC 7 cut(s) 87, 96, 371, 380, 492, 520, 792
BstNI CCWGG 5 cut(s) 194, 224, 362, 380, 745
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 9 cut(s) 42, 178, 192, 222, 360, 378, 447, 654, 743
BstUI CGCG 3 cut(s) 77, 567, 610
BstV2I GAAGAC 1 cut(s) 736
BstX2I RGATCY 1 cut(s) 219
BstYI RGATCY 1 cut(s) 219
BstZI CGGCCG 2 cut(s) 116, 406
BsuRI GGCC 5 cut(s) 118, 198, 365, 383, 408
BtgI CCRYGG 3 cut(s) 565, 608, 762
BtrI CACGTC 1 cut(s) 517
BtsI GCAGTG 2 cut(s) 777, 818
BtsIMutI CAGTG 2 cut(s) 777, 818
BveI ACCTGC 1 cut(s) 384
Cac8I GCNNGC 4 cut(s) 79, 200, 580, 608
CfoI GCGC 2 cut(s) 77, 162
Cfr10I RCCGGY 2 cut(s) 93, 114
Cfr13I GGNCC 5 cut(s) 30, 40, 196, 226, 358
Cfr42I CCGCGG 2 cut(s) 568, 611
CseI GACGC 1 cut(s) 172
Csp6I GTAC 2 cut(s) 154, 562
CspAI ACCGGT 1 cut(s) 93
CviAII CATG 3 cut(s) 151, 169, 322
CviQI GTAC 2 cut(s) 154, 562
DpnI GATC 7 cut(s) 71, 125, 173, 221, 284, 318, 326
DpnII GATC 7 cut(s) 69, 123, 171, 219, 282, 316, 324
DrdI GACNNNNNNGTC 1 cut(s) 738
DseDI GACNNNNNNGTC 1 cut(s) 738
EaeI YGGCCR 2 cut(s) 116, 406
EagI CGGCCG 2 cut(s) 116, 406
EciI GGCGGA 2 cut(s) 20, 763
Ecl136II GAGCTC 1 cut(s) 265
EclXI CGGCCG 2 cut(s) 116, 406
Eco147I AGGCCT 1 cut(s) 383
Eco24I GRGCYC 3 cut(s) 267, 608, 761
Eco47I GGWCC 4 cut(s) 30, 40, 226, 358
Eco52I CGGCCG 2 cut(s) 116, 406
Eco53kI GAGCTC 1 cut(s) 265
Eco72I CACGTG 1 cut(s) 459
EcoICRI GAGCTC 1 cut(s) 265
EcoRII CCWGG 5 cut(s) 192, 222, 360, 378, 743
EcoT38I GRGCYC 3 cut(s) 267, 608, 761
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 3 cut(s) 154, 172, 325
FaqI GGGAC 5 cut(s) 43, 671, 722, 726, 728
FatI CATG 3 cut(s) 150, 168, 321
FauI CCCGC 3 cut(s) 221, 560, 615
FbaI TGATCA 1 cut(s) 171
FblI GTMKAC 2 cut(s) 186, 336
Fnu4HI GCNGC 7 cut(s) 366, 369, 372, 409, 496, 611, 768
FriOI GRGCYC 3 cut(s) 267, 608, 761
Fsp4HI GCNGC 7 cut(s) 366, 369, 372, 409, 496, 611, 768
FspBI CTAG 2 cut(s) 579, 801
GlaI GCGC 2 cut(s) 76, 161
GluI GCNGC 7 cut(s) 366, 369, 372, 409, 496, 611, 768
GsuI CTGGAG 1 cut(s) 362
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 5 cut(s) 118, 198, 365, 383, 408
HgaI GACGC 1 cut(s) 172
HhaI GCGC 2 cut(s) 77, 162
Hin1I GRCGYC 1 cut(s) 183
Hin1II CATG 3 cut(s) 154, 172, 325
Hin6I GCGC 2 cut(s) 75, 160
HinP1I GCGC 2 cut(s) 75, 160
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HinfI GANTC 1 cut(s) 817
HphI GGTGA 2 cut(s) 58, 166
Hpy166II GTNNAC 3 cut(s) 49, 187, 337
Hpy188III TCNNGA 4 cut(s) 28, 67, 286, 821
Hpy8I GTNNAC 3 cut(s) 49, 187, 337
Hpy99I CGWCG 2 cut(s) 124, 188
HpyAV CCTTC 2 cut(s) 31, 773
HpyCH4III ACNGT 2 cut(s) 503, 719
HpyCH4IV ACGT 5 cut(s) 297, 333, 339, 458, 516
HpyCH4V TGCA 3 cut(s) 388, 486, 514
HpyF10VI GCNNNNNNNGC 7 cut(s) 87, 96, 371, 380, 492, 520, 792
HpySE526I ACGT 5 cut(s) 297, 333, 339, 458, 516
Hsp92I GRCGYC 1 cut(s) 183
Hsp92II CATG 3 cut(s) 154, 172, 325
HspAI GCGC 2 cut(s) 75, 160
KpnI GGTACC 1 cut(s) 565
Ksp22I TGATCA 1 cut(s) 171
KspI CCGCGG 2 cut(s) 568, 611
Kzo9I GATC 7 cut(s) 69, 123, 171, 219, 282, 316, 324
MaeI CTAG 2 cut(s) 579, 801
MaeII ACGT 5 cut(s) 297, 333, 339, 458, 516
MaeIII GTNAC 2 cut(s) 83, 436
MalI GATC 7 cut(s) 71, 125, 173, 221, 284, 318, 326
MboI GATC 7 cut(s) 69, 123, 171, 219, 282, 316, 324
MboII GAAGA 3 cut(s) 247, 741, 800
MflI RGATCY 1 cut(s) 219
MhlI GDGCHC 6 cut(s) 12, 101, 267, 608, 654, 761
MluCI AATT 2 cut(s) 505, 536
MmeI TCCRAC 1 cut(s) 171
MspA1I CMGCKG 2 cut(s) 567, 610
MspR9I CCNGG 9 cut(s) 44, 180, 194, 224, 362, 380, 449, 656, 745
MvaI CCWGG 5 cut(s) 194, 224, 362, 380, 745
MvnI CGCG 3 cut(s) 77, 567, 610
MwoI GCNNNNNNNGC 7 cut(s) 87, 96, 371, 380, 492, 520, 792
NciI CCSGG 4 cut(s) 44, 180, 449, 656
NdeII GATC 7 cut(s) 69, 123, 171, 219, 282, 316, 324
NheI GCTAGC 1 cut(s) 578
NlaIII CATG 3 cut(s) 154, 172, 325
NlaIV GGNNCC 6 cut(s) 31, 563, 587, 593, 614, 758
NmeAIII GCCGAG 1 cut(s) 821
NmuCI GTSAC 2 cut(s) 83, 436
NspI RCATGY 1 cut(s) 154
PasI CCCWGGG 1 cut(s) 193
PceI AGGCCT 1 cut(s) 383
PciI ACATGT 1 cut(s) 150
PcsI WCGNNNNNNNCGW 3 cut(s) 128, 264, 282
PfeI GAWTC 1 cut(s) 817
PfoI TCCNGGA 2 cut(s) 222, 447
PinAI ACCGGT 1 cut(s) 93
PkrI GCNGC 7 cut(s) 367, 370, 373, 410, 497, 612, 769
PmaCI CACGTG 1 cut(s) 459
PmlI CACGTG 1 cut(s) 459
Ppu21I YACGTR 1 cut(s) 459
PscI ACATGT 1 cut(s) 150
Psp124BI GAGCTC 1 cut(s) 267
Psp6I CCWGG 5 cut(s) 192, 222, 360, 378, 743
PspCI CACGTG 1 cut(s) 459
PspGI CCWGG 5 cut(s) 192, 222, 360, 378, 743
PspN4I GGNNCC 6 cut(s) 31, 563, 587, 593, 614, 758
PspPI GGNCC 5 cut(s) 30, 40, 196, 226, 358
PsuI RGATCY 1 cut(s) 219
RsaI GTAC 2 cut(s) 155, 563
RsaNI GTAC 2 cut(s) 154, 562
SacI GAGCTC 1 cut(s) 267
SacII CCGCGG 2 cut(s) 568, 611
SalI GTCGAC 1 cut(s) 185
SatI GCNGC 7 cut(s) 366, 369, 372, 409, 496, 611, 768
Sau3AI GATC 7 cut(s) 69, 123, 171, 219, 282, 316, 324
Sau96I GGNCC 5 cut(s) 30, 40, 196, 226, 358
ScrFI CCNGG 9 cut(s) 44, 180, 194, 224, 362, 380, 449, 656, 745
SduI GDGCHC 6 cut(s) 12, 101, 267, 608, 654, 761
Sfr303I CCGCGG 2 cut(s) 568, 611
SgrBI CCGCGG 2 cut(s) 568, 611
SinI GGWCC 4 cut(s) 30, 40, 226, 358
Sse9I AATT 2 cut(s) 505, 536
SseBI AGGCCT 1 cut(s) 383
SspMI CTAG 2 cut(s) 579, 801
SstI GAGCTC 1 cut(s) 267
StuI AGGCCT 1 cut(s) 383
StyD4I CCNGG 9 cut(s) 42, 178, 192, 222, 360, 378, 447, 654, 743
TaaI ACNGT 2 cut(s) 503, 719
TaiI ACGT 5 cut(s) 300, 336, 342, 461, 519
TaqII GACCGA 1 cut(s) 47
TasI AATT 2 cut(s) 505, 536
TauI GCSGC 7 cut(s) 368, 371, 374, 411, 498, 613, 770
TfiI GAWTC 1 cut(s) 817
TscAI CASTG 2 cut(s) 777, 818
TseFI GTSAC 2 cut(s) 83, 436
Tsp45I GTSAC 2 cut(s) 83, 436
TspGWI ACGGA 1 cut(s) 455
TspRI CASTG 2 cut(s) 777, 818
VpaK11BI GGWCC 4 cut(s) 30, 40, 226, 358
XceI RCATGY 1 cut(s) 154
XmiI GTMKAC 2 cut(s) 186, 336
XspI CTAG 2 cut(s) 579, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.