AT5G44730

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012288
Physical Location & Seq
Reverse (-)
18045480 .. 18047075
1596 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44730.2

Sequence Viewer

Length: 768 bp
ATGGCGGTGTCGCTTTTATCGAAACTACGGTGTATAACAGTAGATGTTACTGGTACACTTATAGCTTACAAAGGAGAGCTTGGTGATTACTATTGTATGGCTGCTAAAGCCATTGGTTTGCCTTGTCCTGATTATAAACGAGTTCATGAAGGTTTTAAACTAGCTTATACAGATATGGCACAAAAGTATCCTTGTTTCGGTTTCCATGCCAAAATGCCAAACATTGTTTGGTGGAAAACTTGTGTGAGAGATTCATTTGTCAAGGCAGGATATGAGTATGATGAGGAGACATTTGAGAAGATATTTAGGAGAATCTATTCGACGTTTGGTTCTGCTGCACCTTACTCTGTGTTTCAAGATTCTCAACCGTTTTTAAGATGGGCACGCAGGAAAGGTCTTATAGTCGGACTTGTTAGCAATGCGGAATACCGATATCAAGAAGTTATTTTACCTTCCTTCGGTTTGAGCAAGGCAGAGTGGGATTTTGGTGTATTCTCTGGAATTGAAGGGATAGAGAAACCAGATCCGAGGATTTTTACGCTCGCGCTAGAGAGAGCCGGGAATAATATTGCGCCTGAAGAGGTTTTGCATATTGGAGACAGCATGCGCAAAGATTATGTTCCAGCAAAGAGTATTGGGATGCATGCTTTGTTGGTTGATAGGTTTAAGACGGAAGCTGCTAAAGACTGGATAGAAGCTGGAGCCATTGTGCTTCCAGATTTGGTTGCTGTTCAACAACTTTTGGAGTCTGATAAGTTGAAATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase

Protein Analysis

255

Amino Acids

28.81

Weight (kDa)

6.9

Isoelectric Point (pI)

44.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 10 - 213 9.2e-26 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 83 - 219 5.3e-09 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 173 - 242 4.6e-09 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44730 AT5G44730
fragaria_vesca FvH4_3g13030
malus_domestica MD10G1226900.v1.1
prunus_persica Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1
pyrus_communis pycom10g19080
rosa_chinensis RchiOBHm_Chr5g0021221
rosa_laevigata RLG00000032596
rosa_multiflora Rmu_sc0002467.1_g000017 Rmu_sc0003392.1_g000018
rosa_roxburghii Rroxscaffold_1G00057280
rosa_rugosa Rorug05G0065300
rosa_samantha Rh5AG154300 Rh5BG153900 Rh5CG167500 Rh5DG154400
rosa_wichuraiana Rw5G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 135
Acc16I TGCGCA 1 cut(s) 608
AccII CGCG 1 cut(s) 545
AciI CCGC 2 cut(s) 5, 422
AclWI GGATC 1 cut(s) 518
AcuI CTGAAG 1 cut(s) 597
AfaI GTAC 1 cut(s) 55
AfiI CCNNNNNNNGG 2 cut(s) 197, 458
AgsI TTSAA 4 cut(s) 356, 506, 734, 760
AluBI AGCT 5 cut(s) 65, 79, 164, 677, 698
AluI AGCT 5 cut(s) 65, 79, 164, 677, 698
Alw26I GTCTC 2 cut(s) 281, 591
AlwI GGATC 1 cut(s) 518
ApeKI GCWGC 3 cut(s) 101, 335, 677
Asp700I GAANNNNTTC 1 cut(s) 316
AspLEI GCGC 3 cut(s) 547, 574, 609
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 1 cut(s) 95
BaeGI GKGCMC 1 cut(s) 385
BaeI ACNNNNGTAYC 2 cut(s) 170, 203
BarI GAAGNNNNNNTAC 2 cut(s) 432, 464
BbvI GCAGC 3 cut(s) 88, 322, 664
BccI CCATC 1 cut(s) 372
BciVI GTATCC 1 cut(s) 198
BcnI CCSGG 1 cut(s) 559
BcoDI GTCTC 2 cut(s) 281, 591
BfaI CTAG 2 cut(s) 161, 548
BfuI GTATCC 1 cut(s) 198
BisI GCNGC 3 cut(s) 102, 336, 678
BlsI GCNGC 3 cut(s) 103, 337, 679
Bme1390I CCNGG 1 cut(s) 559
BmiI GGNNCC 1 cut(s) 703
BmrFI CCNGG 1 cut(s) 559
BmsI GCATC 1 cut(s) 630
BpmI CTGGAG 1 cut(s) 720
BpuMI CCSGG 1 cut(s) 559
BsaJI CCNNGG 1 cut(s) 527
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 458
Bse1I ACTGG 2 cut(s) 55, 692
Bse3DI GCAATG 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 527
BseGI GGATG 1 cut(s) 645
BseLI CCNNNNNNNGG 2 cut(s) 197, 458
BseMI GCAATG 1 cut(s) 424
BseNI ACTGG 2 cut(s) 55, 692
BseRI GAGGAG 1 cut(s) 299
BseSI GKGCMC 1 cut(s) 385
BseXI GCAGC 3 cut(s) 88, 322, 664
BsgI GTGCAG 1 cut(s) 321
Bsh1236I CGCG 1 cut(s) 545
BsiSI CCGG 1 cut(s) 558
BslI CCNNNNNNNGG 2 cut(s) 197, 458
BsmAI GTCTC 2 cut(s) 281, 591
Bsp1286I GDGCHC 1 cut(s) 385
Bsp143I GATC 1 cut(s) 523
BspACI CCGC 2 cut(s) 5, 422
BspFNI CGCG 1 cut(s) 545
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 703
BspPI GGATC 1 cut(s) 518
BsrDI GCAATG 1 cut(s) 424
BsrI ACTGG 2 cut(s) 55, 692
BssECI CCNNGG 1 cut(s) 527
BssMI GATC 1 cut(s) 523
Bst4CI ACNGT 3 cut(s) 30, 40, 369
Bst6I CTCTTC 1 cut(s) 573
BstC8I GCNNGC 4 cut(s) 385, 543, 605, 645
BstF5I GGATG 1 cut(s) 645
BstFNI CGCG 1 cut(s) 545
BstHHI GCGC 3 cut(s) 547, 574, 609
BstKTI GATC 1 cut(s) 526
BstMAI GTCTC 2 cut(s) 281, 591
BstMBI GATC 1 cut(s) 523
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNSI RCATGY 2 cut(s) 607, 647
BstSCI CCNGG 1 cut(s) 557
BstSLI GKGCMC 1 cut(s) 385
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 3 cut(s) 88, 322, 664
BstX2I RGATCY 1 cut(s) 523
BstYI RGATCY 1 cut(s) 523
BsuI GTATCC 1 cut(s) 198
BtsCI GGATG 1 cut(s) 645
Cac8I GCNNGC 4 cut(s) 385, 543, 605, 645
CciI TCATGA 1 cut(s) 145
CfoI GCGC 3 cut(s) 547, 574, 609
Csp6I GTAC 1 cut(s) 54
CviAII CATG 4 cut(s) 146, 206, 604, 644
CviJI RGCY 9 cut(s) 65, 79, 101, 110, 164, 557, 677, 698, 704
CviKI_1 RGCY 9 cut(s) 65, 79, 101, 110, 164, 557, 677, 698, 704
CviQI GTAC 1 cut(s) 54
DpnI GATC 1 cut(s) 525
DpnII GATC 1 cut(s) 523
DraI TTTAAA 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
Eco32I GATATC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 597
EcoRV GATATC 1 cut(s) 434
EcoT22I ATGCAT 1 cut(s) 645
FaeI CATG 4 cut(s) 149, 209, 607, 647
FalI AAGNNNNNCTT 2 cut(s) 63, 95
FatI CATG 4 cut(s) 145, 205, 603, 643
Fnu4HI GCNGC 3 cut(s) 102, 336, 678
FokI GGATG 1 cut(s) 652
Fsp4HI GCNGC 3 cut(s) 102, 336, 678
FspBI CTAG 2 cut(s) 161, 548
FspI TGCGCA 1 cut(s) 608
GlaI GCGC 3 cut(s) 546, 573, 608
GluI GCNGC 3 cut(s) 102, 336, 678
GsuI CTGGAG 1 cut(s) 720
HapII CCGG 1 cut(s) 558
HhaI GCGC 3 cut(s) 547, 574, 609
Hin1II CATG 4 cut(s) 149, 209, 607, 647
Hin6I GCGC 3 cut(s) 545, 572, 607
HinP1I GCGC 3 cut(s) 545, 572, 607
HinfI GANTC 4 cut(s) 251, 312, 359, 746
HpaII CCGG 1 cut(s) 558
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 1 cut(s) 56
Hpy188I TCNGA 3 cut(s) 407, 528, 751
Hpy188III TCNNGA 6 cut(s) 128, 146, 356, 437, 498, 716
Hpy8I GTNNAC 1 cut(s) 56
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 4 cut(s) 143, 462, 466, 500
HpyCH4III ACNGT 3 cut(s) 30, 40, 369
HpyCH4IV ACGT 1 cut(s) 323
HpyCH4V TGCA 3 cut(s) 338, 589, 643
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpySE526I ACGT 1 cut(s) 323
Hsp92II CATG 4 cut(s) 149, 209, 607, 647
HspAI GCGC 3 cut(s) 545, 572, 607
Kzo9I GATC 1 cut(s) 523
LmnI GCTCC 1 cut(s) 701
Lsp1109I GCAGC 3 cut(s) 88, 322, 664
LweI GCATC 1 cut(s) 630
MaeI CTAG 2 cut(s) 161, 548
MaeII ACGT 1 cut(s) 323
MaeIII GTNAC 1 cut(s) 46
MalI GATC 1 cut(s) 525
MboI GATC 1 cut(s) 523
MboII GAAGA 2 cut(s) 310, 590
MflI RGATCY 1 cut(s) 523
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 1 cut(s) 501
MlyI GAGTC 1 cut(s) 755
MmeI TCCRAC 1 cut(s) 385
MnlI CCTC 3 cut(s) 277, 522, 574
Mph1103I ATGCAT 1 cut(s) 645
MroXI GAANNNNTTC 1 cut(s) 316
MseI TTAA 4 cut(s) 156, 374, 666, 766
MspI CCGG 1 cut(s) 558
MspR9I CCNGG 1 cut(s) 559
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 1 cut(s) 107
NciI CCSGG 1 cut(s) 559
NdeII GATC 1 cut(s) 523
NlaIII CATG 4 cut(s) 149, 209, 607, 647
NlaIV GGNNCC 1 cut(s) 703
NsbI TGCGCA 1 cut(s) 608
NsiI ATGCAT 1 cut(s) 645
NspI RCATGY 2 cut(s) 607, 647
PaeI GCATGC 2 cut(s) 607, 647
PagI TCATGA 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 316
PfeI GAWTC 3 cut(s) 251, 312, 359
PkrI GCNGC 3 cut(s) 103, 337, 679
PleI GAGTC 1 cut(s) 754
PpsI GAGTC 1 cut(s) 754
PsiI TTATAA 1 cut(s) 135
PspN4I GGNNCC 1 cut(s) 703
PsuI RGATCY 1 cut(s) 523
RsaI GTAC 1 cut(s) 55
RsaNI GTAC 1 cut(s) 54
SaqAI TTAA 4 cut(s) 156, 374, 666, 766
SatI GCNGC 3 cut(s) 102, 336, 678
Sau3AI GATC 1 cut(s) 523
SchI GAGTC 1 cut(s) 755
ScrFI CCNGG 1 cut(s) 559
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 1 cut(s) 630
SphI GCATGC 2 cut(s) 607, 647
Sse9I AATT 1 cut(s) 501
SsiI CCGC 2 cut(s) 5, 422
SspI AATATT 1 cut(s) 568
SspMI CTAG 2 cut(s) 161, 548
StyD4I CCNGG 1 cut(s) 557
TaaI ACNGT 3 cut(s) 30, 40, 369
TaiI ACGT 1 cut(s) 326
TaqI TCGA 2 cut(s) 20, 320
TasI AATT 1 cut(s) 501
TfiI GAWTC 3 cut(s) 251, 312, 359
Tru1I TTAA 4 cut(s) 156, 374, 666, 766
Tru9I TTAA 4 cut(s) 156, 374, 666, 766
TseI GCWGC 3 cut(s) 101, 335, 677
TspDTI ATGAA 3 cut(s) 134, 162, 243
TspGWI ACGGA 1 cut(s) 686
XceI RCATGY 2 cut(s) 607, 647
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
XmnI GAANNNNTTC 1 cut(s) 316
XspI CTAG 2 cut(s) 161, 548
Zsp2I ATGCAT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.