Rh5DG154400

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012288
Physical Location & Seq
Forward (+)
15772045 .. 15773480
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG154400.1

Sequence Viewer

Length: 762 bp
ATGTCATTGTTGTCAAAGTTGCGATGCATCACGGTGGATGTTACTGGTACATTGATAGCTTACAAGGGGGAGCTTGGTGACTACTATTGCATGGCAGCCAAATCCGTGGGGCAGCCATGCCCTGACTACAAAAGGATGCATGAGGGCTTCAAAAATGCTTATAAAGACATGGCCAGCAAATATCCTTGTTTTGGGCATGCTGCCAAAATGCCCAACATCGTATGGTGGAAAACTTGTGTCAGAGATTCCTTTATCAGAGCTGGATATCATTATGATGAGGAGACATTTGAGAAAGTGTTTCGACGCATATATGCATCCTTTGGTTCCGCTGCTCCTTATAGCATCTTTCCCGATTCCCAACCATTTATGAGATGGGTCCGTGAACAAGGTCTTGTAGTTGGAATTGTTAGCAATGCAGAATACCGGTATCAGGATGTTATTCTTCCAGCCTTGGGTTTGAACCAGGGATCTGAATGGGACTTCGGTGTGTTCTCTGGTCTCGAAGGTGTGGAGAAACCAGACCCAAGGATTTATGAGATTGCGCTTAAGAGGGCTGGGAACCTAAAACCAGAAGAGGTTCTGCACATAGGGGACAGCATGCGCAAAGACTTTGTGCCAGCAACAAGTATTGGGATGCATGCACTGTTATTGGACAGGTTTAAGACATCTGATGCTGAGGAATGGAGGAAATCTGGTGCGGTTGTGCTTCCTGATTTGGAAGCAGCAAAAGAATGGCTTACTTCAGAGAAGCCAGCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase

Protein Analysis

253

Amino Acids

28.62

Weight (kDa)

6.53

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 8 - 203 8.1e-24 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 82 - 214 1.2e-09 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 172 - 241 2.9e-08 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44730 AT5G44730
fragaria_vesca FvH4_3g13030
malus_domestica MD10G1226900.v1.1
prunus_persica Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1
pyrus_communis pycom10g19080
rosa_chinensis RchiOBHm_Chr5g0021221
rosa_laevigata RLG00000032596
rosa_multiflora Rmu_sc0002467.1_g000017 Rmu_sc0003392.1_g000018
rosa_roxburghii Rroxscaffold_1G00057280
rosa_rugosa Rorug05G0065300
rosa_samantha Rh5AG154300 Rh5BG153900 Rh5CG167500 Rh5DG154400
rosa_wichuraiana Rw5G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 162
Acc16I TGCGCA 1 cut(s) 602
AciI CCGC 2 cut(s) 327, 698
AclWI GGATC 1 cut(s) 475
AcoI YGGCCR 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 3 cut(s) 191, 430, 452
AflII CTTAAG 1 cut(s) 545
AgeI ACCGGT 1 cut(s) 423
AgsI TTSAA 2 cut(s) 151, 460
AjnI CCWGG 1 cut(s) 462
AluBI AGCT 3 cut(s) 59, 73, 260
AluI AGCT 3 cut(s) 59, 73, 260
Alw26I GTCTC 2 cut(s) 275, 503
AlwI GGATC 1 cut(s) 475
AoxI GGCC 1 cut(s) 171
ApeKI GCWGC 5 cut(s) 95, 112, 200, 329, 722
AsiGI ACCGGT 1 cut(s) 423
Asp700I GAANNNNTTC 1 cut(s) 576
AspLEI GCGC 2 cut(s) 544, 603
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 376
BalI TGGCCA 1 cut(s) 173
BbvCI CCTCAGC 1 cut(s) 675
BbvI GCAGC 5 cut(s) 107, 124, 187, 316, 734
BccI CCATC 1 cut(s) 366
BciT130I CCWGG 1 cut(s) 464
BcoDI GTCTC 2 cut(s) 275, 503
BfrI CTTAAG 1 cut(s) 545
BisI GCNGC 5 cut(s) 96, 113, 201, 330, 723
BlsI GCNGC 5 cut(s) 97, 114, 202, 331, 724
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 3 cut(s) 325, 377, 560
BmrFI CCNGG 1 cut(s) 464
BmsI GCATC 7 cut(s) 14, 36, 126, 323, 351, 624, 661
Bpu10I CCTNAGC 1 cut(s) 675
BsaI GGTCTC 1 cut(s) 503
BsaJI CCNNGG 4 cut(s) 105, 450, 463, 524
BsaWI WCCGGW 1 cut(s) 423
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 430, 452
Bse118I RCCGGY 1 cut(s) 423
Bse1I ACTGG 1 cut(s) 49
Bse3DI GCAATG 1 cut(s) 418
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 4 cut(s) 105, 450, 463, 524
BseGI GGATG 5 cut(s) 43, 141, 314, 439, 639
BseLI CCNNNNNNNGG 3 cut(s) 191, 430, 452
BseMI GCAATG 1 cut(s) 418
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 5 cut(s) 107, 124, 187, 316, 734
BseYI CCCAGC 1 cut(s) 554
BsgI GTGCAG 1 cut(s) 566
BshFI GGCC 1 cut(s) 173
BshTI ACCGGT 1 cut(s) 423
BsiSI CCGG 1 cut(s) 424
BslFI GGGAC 2 cut(s) 491, 605
BslI CCNNNNNNNGG 3 cut(s) 191, 430, 452
BsmAI GTCTC 2 cut(s) 275, 503
BsmFI GGGAC 2 cut(s) 491, 605
BsnI GGCC 1 cut(s) 173
Bso31I GGTCTC 1 cut(s) 503
Bsp143I GATC 1 cut(s) 467
BspACI CCGC 2 cut(s) 327, 698
BspANI GGCC 1 cut(s) 173
BspCNI CTCAG 1 cut(s) 667
BspLI GGNNCC 3 cut(s) 325, 377, 560
BspPI GGATC 1 cut(s) 475
BspTI CTTAAG 1 cut(s) 545
BspTNI GGTCTC 1 cut(s) 503
BsrDI GCAATG 1 cut(s) 418
BsrFI RCCGGY 1 cut(s) 423
BsrI ACTGG 1 cut(s) 49
BssAI RCCGGY 1 cut(s) 423
BssECI CCNNGG 4 cut(s) 105, 450, 463, 524
BssMI GATC 1 cut(s) 467
BssT1I CCWWGG 2 cut(s) 450, 524
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 2 cut(s) 34, 645
Bst6I CTCTTC 1 cut(s) 567
BstAFI CTTAAG 1 cut(s) 545
BstC8I GCNNGC 7 cut(s) 175, 198, 599, 618, 639, 753, 757
BstDEI CTNAG 1 cut(s) 675
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 5 cut(s) 43, 141, 314, 439, 639
BstHHI GCGC 2 cut(s) 544, 603
BstKTI GATC 1 cut(s) 470
BstMAI GTCTC 2 cut(s) 275, 503
BstMBI GATC 1 cut(s) 467
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 4 cut(s) 200, 601, 641, 759
BstSCI CCNGG 1 cut(s) 462
BstV1I GCAGC 5 cut(s) 107, 124, 187, 316, 734
BstX2I RGATCY 1 cut(s) 467
BstXI CCANNNNNNTGG 1 cut(s) 106
BstYI RGATCY 1 cut(s) 467
BsuRI GGCC 1 cut(s) 173
BtgI CCRYGG 1 cut(s) 105
BtgZI GCGATG 1 cut(s) 37
BtsCI GGATG 5 cut(s) 43, 141, 314, 439, 639
BtsIMutI CAGTG 1 cut(s) 641
Cac8I GCNNGC 7 cut(s) 175, 198, 599, 618, 639, 753, 757
CfoI GCGC 2 cut(s) 544, 603
Cfr10I RCCGGY 1 cut(s) 423
Cfr13I GGNCC 1 cut(s) 376
CseI GACGC 1 cut(s) 312
Csp6I GTAC 1 cut(s) 48
CspAI ACCGGT 1 cut(s) 423
CviAII CATG 8 cut(s) 91, 117, 140, 169, 197, 598, 638, 756
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 1 cut(s) 675
DpnI GATC 1 cut(s) 469
DpnII GATC 1 cut(s) 467
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Eco130I CCWWGG 2 cut(s) 450, 524
Eco31I GGTCTC 1 cut(s) 503
Eco32I GATATC 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 726
EcoRII CCWGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 266
EcoT14I CCWWGG 2 cut(s) 450, 524
EcoT22I ATGCAT 4 cut(s) 29, 141, 316, 639
ErhI CCWWGG 2 cut(s) 450, 524
FaeI CATG 8 cut(s) 94, 120, 143, 172, 200, 601, 641, 759
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 2 cut(s) 491, 605
FatI CATG 8 cut(s) 90, 116, 139, 168, 196, 597, 637, 755
Fnu4HI GCNGC 5 cut(s) 96, 113, 201, 330, 723
FokI GGATG 5 cut(s) 50, 148, 301, 446, 646
Fsp4HI GCNGC 5 cut(s) 96, 113, 201, 330, 723
FspI TGCGCA 1 cut(s) 602
GlaI GCGC 2 cut(s) 543, 602
GluI GCNGC 5 cut(s) 96, 113, 201, 330, 723
GsaI CCCAGC 1 cut(s) 558
HaeIII GGCC 1 cut(s) 173
HapII CCGG 1 cut(s) 424
HgaI GACGC 1 cut(s) 312
HhaI GCGC 2 cut(s) 544, 603
Hin1II CATG 8 cut(s) 94, 120, 143, 172, 200, 601, 641, 759
Hin6I GCGC 2 cut(s) 542, 601
HinP1I GCGC 2 cut(s) 542, 601
HinfI GANTC 2 cut(s) 245, 353
HpaII CCGG 1 cut(s) 424
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 1 cut(s) 383
Hpy188I TCNGA 5 cut(s) 242, 257, 472, 670, 745
Hpy188III TCNNGA 4 cut(s) 350, 431, 500, 710
Hpy8I GTNNAC 1 cut(s) 383
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 2 cut(s) 34, 645
HpyCH4V TGCA 8 cut(s) 27, 90, 139, 314, 416, 583, 637, 641
HpyF3I CTNAG 1 cut(s) 675
Hsp92II CATG 8 cut(s) 94, 120, 143, 172, 200, 601, 641, 759
HspAI GCGC 2 cut(s) 542, 601
Kzo9I GATC 1 cut(s) 467
LmnI GCTCC 2 cut(s) 70, 337
Lsp1109I GCAGC 5 cut(s) 107, 124, 187, 316, 734
LweI GCATC 7 cut(s) 14, 36, 126, 323, 351, 624, 661
MaeIII GTNAC 2 cut(s) 40, 77
MalI GATC 1 cut(s) 469
MboI GATC 1 cut(s) 467
MboII GAAGA 2 cut(s) 434, 584
MflI RGATCY 1 cut(s) 467
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 1 cut(s) 402
MluNI TGGCCA 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 6 cut(s) 136, 271, 543, 568, 670, 678
Mox20I TGGCCA 1 cut(s) 173
Mph1103I ATGCAT 4 cut(s) 29, 141, 316, 639
MroXI GAANNNNTTC 1 cut(s) 576
MscI TGGCCA 1 cut(s) 173
MseI TTAA 2 cut(s) 546, 660
MslI CAYNNNNRTG 2 cut(s) 32, 273
Msp20I TGGCCA 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 329
MspCI CTTAAG 1 cut(s) 545
MspI CCGG 1 cut(s) 424
MspR9I CCNGG 1 cut(s) 464
MvaI CCWGG 1 cut(s) 464
NdeII GATC 1 cut(s) 467
NlaIII CATG 8 cut(s) 94, 120, 143, 172, 200, 601, 641, 759
NlaIV GGNNCC 3 cut(s) 325, 377, 560
NmuCI GTSAC 1 cut(s) 77
NsbI TGCGCA 1 cut(s) 602
NsiI ATGCAT 4 cut(s) 29, 141, 316, 639
NspI RCATGY 4 cut(s) 200, 601, 641, 759
PaeI GCATGC 4 cut(s) 200, 601, 641, 759
PdmI GAANNNNTTC 1 cut(s) 576
PfeI GAWTC 2 cut(s) 245, 353
PinAI ACCGGT 1 cut(s) 423
PkrI GCNGC 5 cut(s) 97, 114, 202, 331, 724
PsiI TTATAA 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 554
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 3 cut(s) 325, 377, 560
PspPI GGNCC 1 cut(s) 376
PsuI RGATCY 1 cut(s) 467
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 2 cut(s) 32, 273
SaqAI TTAA 2 cut(s) 546, 660
SatI GCNGC 5 cut(s) 96, 113, 201, 330, 723
Sau3AI GATC 1 cut(s) 467
Sau96I GGNCC 1 cut(s) 376
ScrFI CCNGG 1 cut(s) 464
SetI ASST 8 cut(s) 61, 75, 262, 391, 508, 564, 579, 659
SfaNI GCATC 7 cut(s) 14, 36, 126, 323, 351, 624, 661
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 2 cut(s) 32, 273
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
SphI GCATGC 4 cut(s) 200, 601, 641, 759
Sse9I AATT 1 cut(s) 402
SsiI CCGC 2 cut(s) 327, 698
StyD4I CCNGG 1 cut(s) 462
StyI CCWWGG 2 cut(s) 450, 524
TaaI ACNGT 2 cut(s) 34, 645
TaqI TCGA 2 cut(s) 301, 501
TasI AATT 1 cut(s) 402
TfiI GAWTC 2 cut(s) 245, 353
Tru1I TTAA 2 cut(s) 546, 660
Tru9I TTAA 2 cut(s) 546, 660
TscAI CASTG 1 cut(s) 648
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 5 cut(s) 95, 112, 200, 329, 722
Tsp45I GTSAC 1 cut(s) 77
TspGWI ACGGA 2 cut(s) 94, 368
TspRI CASTG 1 cut(s) 648
Vha464I CTTAAG 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 376
XceI RCATGY 4 cut(s) 200, 601, 641, 759
XcmI CCANNNNNNNNNTGG 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 576
Zsp2I ATGCAT 4 cut(s) 29, 141, 316, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.