MD10G1226900.v1.1

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0012288
Physical Location & Seq
Reverse (-)
32378125 .. 32381207
3083 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1226900.v1.1.491

Sequence Viewer

Length: 762 bp
ATGTCGTTGTTGTCAAAGTTGCGGTGCATTACAGTGGATGTTACTGGCACATTGCTAGCTTACAAGGGGGAGCTTGGTGACTACTATTGCATGGCAGCCAAAGCTGTAGGGCTGGAATGCCCTGACTACAAAAGGGTGCATGAGGGCTTCAAATATGCATATAAAGACATGGCCACCAAGTATCCATGTTTCGGGCATGCTGCGAAAATGCCCAACATCGTATGGTGGAAAACTTGTGTCAGAGACTCCTTTATAAGGGCTGGATATCATTACGATGAGGAGACATTTGAGAAAGTGTTCCGACGTATATATGCATCCTTTGGTTCCTCTGCTCCTTATTCCATCTTTCCTGACTCCCAACCCTTTCTAAGATGGGTCCATGAACAAGGTCTTAAAGTTGGAATTATTAGCAATGCAGAATACCGTTATCGAGATGTAATTCTTCCAGCCTTGGGTGTGCACCAGGGAACTGATTGGGATTTTGGAGTGTTCTCTGGTATAGAAGGTGTGGAGAAACCAAATCCGAAGATTTATGAGCTTGCGCTCAAAAGGGCTGGAAATCTGTCACCGGACGAGGTTCTGCACATAGGGGACAGCATGCGCAAAGATTATGTCCCAGCAACAAGTATTGGGATGCATGCATTGTTATTGGATAGGTTCAAGACATCTGATGCCGATGAGTGGAGGAAATCTGGCGCAGTTGTCCTTCCCGACTTGGAAGCTGCAAAAGAATGGCTTACTTCACAGAAGTCAGCAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase

Protein Analysis

254

Amino Acids

28.6

Weight (kDa)

7.04

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 8 - 204 3.2e-24 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 82 - 214 1.1e-09 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 172 - 241 1.8e-08 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44730 AT5G44730
fragaria_vesca FvH4_3g13030
malus_domestica MD10G1226900.v1.1
prunus_persica Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1
pyrus_communis pycom10g19080
rosa_chinensis RchiOBHm_Chr5g0021221
rosa_laevigata RLG00000032596
rosa_multiflora Rmu_sc0002467.1_g000017 Rmu_sc0003392.1_g000018
rosa_roxburghii Rroxscaffold_1G00057280
rosa_rugosa Rorug05G0065300
rosa_samantha Rh5AG154300 Rh5BG153900 Rh5CG167500 Rh5DG154400
rosa_wichuraiana Rw5G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
Acc16I TGCGCA 1 cut(s) 602
AciI CCGC 1 cut(s) 22
AcoI YGGCCR 1 cut(s) 171
AfiI CCNNNNNNNGG 4 cut(s) 191, 255, 452, 681
AgsI TTSAA 2 cut(s) 151, 661
AjnI CCWGG 1 cut(s) 462
AluBI AGCT 6 cut(s) 59, 73, 104, 538, 722, 759
AluI AGCT 6 cut(s) 59, 73, 104, 538, 722, 759
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 2 cut(s) 237, 275
Alw44I GTGCAC 1 cut(s) 458
AoxI GGCC 1 cut(s) 171
ApaLI GTGCAC 1 cut(s) 458
ApeKI GCWGC 3 cut(s) 95, 200, 722
ArsI GACNNNNNNTTYG 2 cut(s) 597, 629
Asp700I GAANNNNTTC 1 cut(s) 296
AspLEI GCGC 3 cut(s) 544, 603, 698
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 2 cut(s) 89, 558
AsuNHI GCTAGC 1 cut(s) 55
AvaII GGWCC 1 cut(s) 376
BaeGI GKGCMC 1 cut(s) 462
BalI TGGCCA 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 3 cut(s) 107, 187, 709
BccI CCATC 2 cut(s) 350, 366
BciT130I CCWGG 1 cut(s) 464
BciVI GTATCC 1 cut(s) 192
BcoDI GTCTC 2 cut(s) 237, 275
BfaI CTAG 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 105
BfuI GTATCC 1 cut(s) 192
BisI GCNGC 3 cut(s) 96, 201, 723
BlsI GCNGC 3 cut(s) 97, 202, 724
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 2 cut(s) 325, 377
BmrFI CCNGG 1 cut(s) 464
BmsI GCATC 3 cut(s) 323, 624, 661
BmtI GCTAGC 1 cut(s) 59
BsaJI CCNNGG 2 cut(s) 450, 463
BsaWI WCCGGW 1 cut(s) 568
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 255, 452, 681
Bse1I ACTGG 1 cut(s) 49
Bse3DI GCAATG 2 cut(s) 50, 418
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 2 cut(s) 450, 463
BseGI GGATG 3 cut(s) 43, 314, 639
BseLI CCNNNNNNNGG 4 cut(s) 191, 255, 452, 681
BseMI GCAATG 2 cut(s) 50, 418
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 1 cut(s) 293
BseSI GKGCMC 1 cut(s) 462
BseXI GCAGC 3 cut(s) 107, 187, 709
BseYI CCCAGC 1 cut(s) 616
BsgI GTGCAG 1 cut(s) 566
BshFI GGCC 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 462
BsiSI CCGG 1 cut(s) 569
BslFI GGGAC 2 cut(s) 599, 605
BslI CCNNNNNNNGG 4 cut(s) 191, 255, 452, 681
BsmAI GTCTC 2 cut(s) 237, 275
BsmFI GGGAC 2 cut(s) 599, 605
BsmI GAATGC 1 cut(s) 122
BsnI GGCC 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 462
BspACI CCGC 1 cut(s) 22
BspANI GGCC 1 cut(s) 173
BspLI GGNNCC 2 cut(s) 325, 377
BspOI GCTAGC 1 cut(s) 59
BsrDI GCAATG 2 cut(s) 50, 418
BsrI ACTGG 1 cut(s) 49
BssECI CCNNGG 2 cut(s) 450, 463
BssT1I CCWWGG 1 cut(s) 450
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 2 cut(s) 34, 425
BstC8I GCNNGC 6 cut(s) 57, 198, 540, 599, 639, 757
BstDEI CTNAG 1 cut(s) 368
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 3 cut(s) 43, 314, 639
BstHHI GCGC 3 cut(s) 544, 603, 698
BstMAI GTCTC 2 cut(s) 237, 275
BstMWI GCNNNNNNNGC 1 cut(s) 101
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 3 cut(s) 200, 601, 641
BstSCI CCNGG 1 cut(s) 462
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 1 cut(s) 462
BstV1I GCAGC 3 cut(s) 107, 187, 709
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 1 cut(s) 173
BtsCI GGATG 3 cut(s) 43, 314, 639
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 6 cut(s) 57, 198, 540, 599, 639, 757
CfoI GCGC 3 cut(s) 544, 603, 698
Cfr13I GGNCC 1 cut(s) 376
CviAII CATG 8 cut(s) 91, 140, 169, 186, 197, 380, 598, 638
DdeI CTNAG 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 450
Eco32I GATATC 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 376
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 450
EcoT22I ATGCAT 4 cut(s) 160, 316, 639, 643
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 8 cut(s) 94, 143, 172, 189, 200, 383, 601, 641
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 2 cut(s) 599, 605
FatI CATG 8 cut(s) 90, 139, 168, 185, 196, 379, 597, 637
Fnu4HI GCNGC 3 cut(s) 96, 201, 723
FokI GGATG 3 cut(s) 50, 301, 646
Fsp4HI GCNGC 3 cut(s) 96, 201, 723
FspBI CTAG 1 cut(s) 56
FspI TGCGCA 1 cut(s) 602
GlaI GCGC 3 cut(s) 543, 602, 697
GluI GCNGC 3 cut(s) 96, 201, 723
GsaI CCCAGC 1 cut(s) 620
HaeIII GGCC 1 cut(s) 173
HapII CCGG 1 cut(s) 569
HhaI GCGC 3 cut(s) 544, 603, 698
Hin1II CATG 8 cut(s) 94, 143, 172, 189, 200, 383, 601, 641
Hin6I GCGC 3 cut(s) 542, 601, 696
HinP1I GCGC 3 cut(s) 542, 601, 696
HinfI GANTC 2 cut(s) 245, 353
HpaII CCGG 1 cut(s) 569
HphI GGTGA 2 cut(s) 89, 558
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 4 cut(s) 242, 302, 525, 670
Hpy188III TCNNGA 4 cut(s) 350, 431, 661, 710
Hpy8I GTNNAC 1 cut(s) 460
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 2 cut(s) 497, 716
HpyCH4III ACNGT 2 cut(s) 34, 425
HpyCH4IV ACGT 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 101
HpyF3I CTNAG 1 cut(s) 368
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 8 cut(s) 94, 143, 172, 189, 200, 383, 601, 641
HspAI GCGC 3 cut(s) 542, 601, 696
LmnI GCTCC 2 cut(s) 70, 337
Lsp1109I GCAGC 3 cut(s) 107, 187, 709
LweI GCATC 3 cut(s) 323, 624, 661
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 3 cut(s) 40, 77, 564
MboII GAAGA 2 cut(s) 434, 538
MhlI GDGCHC 1 cut(s) 462
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 2 cut(s) 402, 438
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 2 cut(s) 239, 347
MmeI TCCRAC 2 cut(s) 325, 379
MnlI CCTC 5 cut(s) 136, 271, 337, 568, 678
Mox20I TGGCCA 1 cut(s) 173
Mph1103I ATGCAT 4 cut(s) 160, 316, 639, 643
MroXI GAANNNNTTC 1 cut(s) 296
MscI TGGCCA 1 cut(s) 173
MseI TTAA 1 cut(s) 393
MslI CAYNNNNRTG 2 cut(s) 32, 273
Msp20I TGGCCA 1 cut(s) 173
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 1 cut(s) 464
Mva1269I GAATGC 1 cut(s) 122
MvaI CCWGG 1 cut(s) 464
MwoI GCNNNNNNNGC 1 cut(s) 101
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 8 cut(s) 94, 143, 172, 189, 200, 383, 601, 641
NlaIV GGNNCC 2 cut(s) 325, 377
NmuCI GTSAC 2 cut(s) 77, 564
NsbI TGCGCA 1 cut(s) 602
NsiI ATGCAT 4 cut(s) 160, 316, 639, 643
NspI RCATGY 3 cut(s) 200, 601, 641
PaeI GCATGC 3 cut(s) 200, 601, 641
PctI GAATGC 1 cut(s) 122
PdmI GAANNNNTTC 1 cut(s) 296
PkrI GCNGC 3 cut(s) 97, 202, 724
PleI GAGTC 2 cut(s) 239, 347
PpsI GAGTC 2 cut(s) 239, 347
PsiI TTATAA 1 cut(s) 254
Psp6I CCWGG 1 cut(s) 462
PspFI CCCAGC 1 cut(s) 616
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 2 cut(s) 325, 377
PspPI GGNCC 1 cut(s) 376
RseI CAYNNNNRTG 2 cut(s) 32, 273
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 3 cut(s) 96, 201, 723
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 2 cut(s) 239, 347
ScrFI CCNGG 1 cut(s) 464
SduI GDGCHC 1 cut(s) 462
SfaNI GCATC 3 cut(s) 323, 624, 661
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 2 cut(s) 32, 273
SphI GCATGC 3 cut(s) 200, 601, 641
Sse9I AATT 2 cut(s) 402, 438
SsiI CCGC 1 cut(s) 22
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 462
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 2 cut(s) 34, 425
TaiI ACGT 1 cut(s) 307
TaqI TCGA 1 cut(s) 430
TasI AATT 2 cut(s) 402, 438
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 2 cut(s) 77, 564
TseI GCWGC 3 cut(s) 95, 200, 722
Tsp45I GTSAC 2 cut(s) 77, 564
TspDTI ATGAA 1 cut(s) 396
TspRI CASTG 1 cut(s) 39
VneI GTGCAC 1 cut(s) 458
VpaK11BI GGWCC 1 cut(s) 376
XagI CCTNNNNNAGG 1 cut(s) 253
XceI RCATGY 3 cut(s) 200, 601, 641
XmnI GAANNNNTTC 1 cut(s) 296
XspI CTAG 1 cut(s) 56
Zsp2I ATGCAT 4 cut(s) 160, 316, 639, 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.