Rorug05G0065300
ERF Family

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0012288
Physical Location & Seq
Forward (+)
5659220 .. 5663430
4211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0065300.1

Sequence Viewer

Length: 1827 bp
ATGGCCCTCAGCGTGTACCGGCGCGACCCGCAGTCGGCGGCCGAGAAGGCCGTCGCGGCAATCGGGTCGGGTTACGACTTCTGCAACGATCTCCGGTTGACTTCGTGCAAGTCCGTACTGATCGAGTTGGATCCGACCCGGACCCGGGACCTCGTCATCCCCGGCGGCCTCGTCGTGCCTGACGTGTCCACCTCTATCAAGTGCGATAAGGGCGAGCGCACCAAGTTCCGCTCCGACGTCGTTTCGTTCAATCAGATGTCAGAGCAGTACAATAAGGAACTTTCTTTATCGGGGAAAATTCCGTCCGGCTTGTTTAATGCTATGTTTAACTACACCGGCTGCTGGCAGAAGGATGCAGCGCCCACAAAATGTCTTGCCTTTGACGGTTGGTTGATAACTCTGTATAACATTGAGTTAGCGAGATCCCAATTGACACTATCGGAGCAAGTGAAACAAGACGTGCCTTCTGTTTGGGACCCTGCTGGCCTTGCCGCGTTCATTGAAAAGTATGGTACTCACATTGTTGTTGGGGTGAAGATGGGTGGAAAGGATATAGTTCACGTAAAGCAGTTACAAAACTCAAAACTTGAGCCCACTGAACTGCAGAAATTGTTAAAGCAATTAGCTGAAGAGAGATTTTCTGATAATGCAAATGAATCTTCTATATCAAATACTGCTGATCTATTAGGAAAAATGAGGGATGACCATCCTGTTCACTGGGATATTCAGAGAACATTAGCATCCTCAGTAAGGCCACCACCCATCATCACTCACTCAAGGAATGATGATATTGTTACCATTAGTGTTCGAAGGGGAGGTGTTGATGTTGGTCAAAGTCATAACCAATGGCTTTCGACTATATCACAGGCGCCTGATGTCATATCAATGTCCTTTGTGCCCATAACATCACTTTTGGGTGGTATTCAAGGCAATGGATTTCTTAGCCATGCAGTAAATCTGTATCTTAGATACAAACCACCAATAGAGGAGCTTCAACAATTCTTAGAATTTCAATTACCACGGCAATGGGCTCCAGTATACGCTGATCTGCCTCTTGGTCTAAGGCGGAGAAGACATGCATCCCCATCACTTCAGTTCAGTTTTATGGGCCCCAAGCTATACGTTAACACTATGAAGGTTGATTCTGTAAATCGACCGGTTACAGGAATCCGTTTGTACCTTGAAGGTAAAAAAGGTGACCATATGGCAATCCATCTTCAGCATCTTACAAGTCTTCCCAGTTCCTTCCAAGTCTCAACTGATCACAATTATGAGCCCATTGACGAGCCAGAGGAACGAGGCTACTTTGAACCTGTCAAGTGGAGCATATTTTCACATGTATGCACTGCCCCAGTACAGTATAATGGTGCCTGCATCAATGACTCTGCTTCTATTGTGACAAAGGCCTGGTTTGAAGTCAAGGCTATTGGAATGAGGAAAGTTTTGTTCCTGAGGCTTGGATTTTCTACTGTGGCATCTGCAAGGATCCGAAGATCAGAATGGGAAGGCTCAAGCTCATCACGTAAGTCAGGATTCATCTCAACCTTGATCAGCACAAGGCTTAGTGCTGCGCTCAATCCACCGGTGGCAGCACCACCAAAAGTGGATTTGAATTCAGCAGTGTATCCTGGTGGCCCACCTTTACCTTCCAAGGCACCAAAAATGGCGACGTTTGTAGATACCAAGGAAATGTTGAGAGGTCCTGAGGACACCCCAGGATATTGGATGGTTACCGGAGCCAAGCTTTGTGTAGAGGGTGGTCGAATCTCAGTCAAAGTGAAGTATTCATTGCTGACAATTATGTCAGAAGACTCGATGATGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase

Protein Analysis

608

Amino Acids

67.42

Weight (kDa)

8.79

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MACPF PF01823 104 - 321 2.6e-34 MAC/Perforin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44730 AT5G44730
fragaria_vesca FvH4_3g13030
malus_domestica MD10G1226900.v1.1
prunus_persica Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1
pyrus_communis pycom10g19080
rosa_chinensis RchiOBHm_Chr5g0021221
rosa_laevigata RLG00000032596
rosa_multiflora Rmu_sc0002467.1_g000017 Rmu_sc0003392.1_g000018
rosa_roxburghii Rroxscaffold_1G00057280
rosa_rugosa Rorug05G0065300
rosa_samantha Rh5AG154300 Rh5BG153900 Rh5CG167500 Rh5DG154400
rosa_wichuraiana Rw5G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1801
AatII GACGTC 1 cut(s) 240
AccB1I GGYRCC 3 cut(s) 868, 1367, 1654
AccBSI CCGCTC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 1038
AccII CGCG 3 cut(s) 24, 56, 494
AciI CCGC 7 cut(s) 29, 38, 56, 165, 229, 492, 1066
AclWI GGATC 5 cut(s) 125, 138, 417, 1480, 1493
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 3 cut(s) 297, 1007, 1612
AcuI CTGAAG 3 cut(s) 648, 1076, 1202
AcyI GRCGYC 2 cut(s) 237, 869
AfaI GTAC 6 cut(s) 17, 117, 269, 514, 1178, 1356
AfiI CCNNNNNNNGG 6 cut(s) 34, 144, 145, 342, 750, 1163
AflIII ACRYGT 2 cut(s) 183, 1336
AgeI ACCGGT 2 cut(s) 1156, 1582
AgsI TTSAA 9 cut(s) 250, 503, 926, 995, 1013, 1184, 1310, 1415, 1612
AhdI GACNNNNNGTC 1 cut(s) 31
AjiI CACGTC 2 cut(s) 184, 460
AjnI CCWGG 3 cut(s) 1406, 1627, 1714
AluBI AGCT 5 cut(s) 626, 991, 1117, 1515, 1744
AluI AGCT 5 cut(s) 626, 991, 1117, 1515, 1744
Alw26I GTCTC 1 cut(s) 1258
AlwI GGATC 5 cut(s) 125, 138, 417, 1480, 1493
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 9 cut(s) 3, 39, 48, 166, 484, 752, 1108, 1404, 1633
ApaI GGGCCC 1 cut(s) 1112
ApeKI GCWGC 4 cut(s) 339, 356, 1568, 1589
ApoI RAATTY 3 cut(s) 297, 1007, 1612
AsiGI ACCGGT 2 cut(s) 1156, 1582
AspLEI GCGC 5 cut(s) 24, 219, 361, 871, 1573
AspS9I GGNCC 8 cut(s) 4, 141, 148, 475, 1108, 1109, 1634, 1700
AsuC2I CCSGG 4 cut(s) 139, 145, 146, 162
AsuHPI GGTGA 2 cut(s) 544, 1208
AsuII TTCGAA 1 cut(s) 808
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 4 cut(s) 141, 148, 475, 1700
AxyI CCTNAGG 2 cut(s) 1451, 1704
BaeGI GKGCMC 2 cut(s) 900, 1112
BamHI GGATCC 2 cut(s) 130, 1485
BanI GGYRCC 3 cut(s) 868, 1367, 1654
BanII GRGCYC 4 cut(s) 594, 1033, 1112, 1278
BbsI GAAGAC 3 cut(s) 1078, 1226, 1815
BbvCI CCTCAGC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 326, 368, 1555, 1601
BccI CCATC 6 cut(s) 532, 714, 770, 1093, 1221, 1720
BceAI ACGGC 2 cut(s) 35, 1037
BcgI CGANNNNNNTGC 2 cut(s) 48, 82
BciT130I CCWGG 3 cut(s) 1408, 1629, 1716
BciVI GTATCC 1 cut(s) 1635
BclI TGATCA 2 cut(s) 1261, 1548
BcnI CCSGG 4 cut(s) 139, 145, 146, 162
BcoDI GTCTC 1 cut(s) 1258
BfmI CTRYAG 1 cut(s) 602
BfoI RGCGCY 2 cut(s) 362, 872
BfuI GTATCC 1 cut(s) 1635
BglI GCCNNNNNGGC 2 cut(s) 47, 56
BisI GCNGC 8 cut(s) 39, 57, 166, 340, 357, 492, 1569, 1590
BlsI GCNGC 8 cut(s) 40, 58, 167, 341, 358, 493, 1570, 1591
Bme1390I CCNGG 7 cut(s) 139, 145, 146, 162, 1408, 1629, 1716
Bme18I GGWCC 4 cut(s) 141, 148, 475, 1700
BmeRI GACNNNNNGTC 1 cut(s) 31
BmeT110I CYCGRG 1 cut(s) 144
BmgBI CACGTC 2 cut(s) 184, 460
BmgT120I GGNCC 8 cut(s) 4, 141, 148, 475, 1108, 1109, 1634, 1700
BmrFI CCNGG 7 cut(s) 139, 145, 146, 162, 1408, 1629, 1716
BmrI ACTGGG 3 cut(s) 727, 1233, 1346
BmsI GCATC 6 cut(s) 343, 749, 1088, 1231, 1383, 1484
BmuI ACTGGG 3 cut(s) 727, 1233, 1346
BpiI GAAGAC 3 cut(s) 1078, 1226, 1815
BpmI CTGGAG 1 cut(s) 1017
Bpu10I CCTNAGC 1 cut(s) 8
Bpu14I TTCGAA 1 cut(s) 808
BpuEI CTTGAG 3 cut(s) 608, 760, 1495
BpuMI CCSGG 4 cut(s) 139, 145, 146, 162
BsaAI YACGTR 2 cut(s) 562, 1523
BsaBI GATNNNNATC 1 cut(s) 705
BsaHI GRCGYC 2 cut(s) 237, 869
BsaJI CCNNGG 6 cut(s) 144, 160, 1019, 1650, 1683, 1714
BsaWI WCCGGW 4 cut(s) 93, 1156, 1582, 1733
Bsc4I CCNNNNNNNGG 6 cut(s) 34, 144, 145, 342, 750, 1163
Bse118I RCCGGY 4 cut(s) 18, 335, 1156, 1582
Bse1I ACTGG 4 cut(s) 722, 1034, 1239, 1352
Bse21I CCTNAGG 2 cut(s) 1451, 1704
Bse3DI GCAATG 3 cut(s) 937, 1031, 1787
Bse8I GATNNNNATC 1 cut(s) 705
BseBI CCWGG 3 cut(s) 1408, 1629, 1716
BseDI CCNNGG 6 cut(s) 144, 160, 1019, 1650, 1683, 1714
BseGI GGATG 7 cut(s) 156, 358, 706, 706, 740, 1079, 1731
BseJI GATNNNNATC 1 cut(s) 705
BseLI CCNNNNNNNGG 6 cut(s) 34, 144, 145, 342, 750, 1163
BseMI GCAATG 3 cut(s) 937, 1031, 1787
BseMII CTCAG 5 cut(s) 22, 759, 1442, 1695, 1782
BseNI ACTGG 4 cut(s) 722, 1034, 1239, 1352
BseRI GAGGAG 1 cut(s) 1001
BseSI GKGCMC 2 cut(s) 900, 1112
BseX3I CGGCCG 1 cut(s) 39
BseXI GCAGC 4 cut(s) 326, 368, 1555, 1601
Bsh1236I CGCG 3 cut(s) 24, 56, 494
Bsh1285I CGRYCG 2 cut(s) 42, 1157
BshFI GGCC 9 cut(s) 5, 41, 50, 168, 486, 754, 1110, 1406, 1635
BshNI GGYRCC 3 cut(s) 868, 1367, 1654
BshTI ACCGGT 2 cut(s) 1156, 1582
BsiEI CGRYCG 2 cut(s) 42, 1157
BsiHKCI CYCGRG 1 cut(s) 144
BslFI GGGAC 2 cut(s) 161, 488
BslI CCNNNNNNNGG 6 cut(s) 34, 144, 145, 342, 750, 1163
BsmAI GTCTC 1 cut(s) 1258
BsmFI GGGAC 2 cut(s) 161, 488
BsnI GGCC 9 cut(s) 5, 41, 50, 168, 486, 754, 1110, 1406, 1635
BsoBI CYCGRG 1 cut(s) 144
Bsp119I TTCGAA 1 cut(s) 808
Bsp120I GGGCCC 1 cut(s) 1108
Bsp1286I GDGCHC 5 cut(s) 594, 900, 1033, 1112, 1278
BspACI CCGC 7 cut(s) 29, 38, 56, 165, 229, 492, 1066
BspANI GGCC 9 cut(s) 5, 41, 50, 168, 486, 754, 1110, 1406, 1635
BspCNI CTCAG 5 cut(s) 21, 758, 1443, 1696, 1781
BspFNI CGCG 3 cut(s) 24, 56, 494
BspMAI CTGCAG 1 cut(s) 606
BspPI GGATC 5 cut(s) 125, 138, 417, 1480, 1493
BspT104I TTCGAA 1 cut(s) 808
BspT107I GGYRCC 3 cut(s) 868, 1367, 1654
BsrBI CCGCTC 1 cut(s) 231
BsrDI GCAATG 3 cut(s) 937, 1031, 1787
BsrFI RCCGGY 4 cut(s) 18, 335, 1156, 1582
BsrI ACTGG 4 cut(s) 722, 1034, 1239, 1352
BssAI RCCGGY 4 cut(s) 18, 335, 1156, 1582
BssECI CCNNGG 6 cut(s) 144, 160, 1019, 1650, 1683, 1714
BssNAI GTATAC 1 cut(s) 1039
BssNI GRCGYC 2 cut(s) 237, 869
BssT1I CCWWGG 2 cut(s) 1650, 1683
Bst1107I GTATAC 1 cut(s) 1039
Bst2UI CCWGG 3 cut(s) 1408, 1629, 1716
Bst4CI ACNGT 3 cut(s) 386, 1359, 1471
Bst6I CTCTTC 1 cut(s) 624
BstACI GRCGYC 2 cut(s) 237, 869
BstBAI YACGTR 2 cut(s) 562, 1523
BstBI TTCGAA 1 cut(s) 808
BstC8I GCNNGC 4 cut(s) 215, 344, 484, 1372
BstDSI CCRYGG 1 cut(s) 1019
BstEII GGTNACC 2 cut(s) 1196, 1729
BstENI CCTNNNNNAGG 1 cut(s) 748
BstF5I GGATG 7 cut(s) 156, 358, 706, 706, 740, 1079, 1731
BstFNI CGCG 3 cut(s) 24, 56, 494
BstH2I RGCGCY 2 cut(s) 362, 872
BstHHI GCGC 5 cut(s) 24, 219, 361, 871, 1573
BstMAI GTCTC 1 cut(s) 1258
BstMCI CGRYCG 2 cut(s) 42, 1157
BstMWI GCNNNNNNNGC 5 cut(s) 28, 47, 56, 210, 488
BstNI CCWGG 3 cut(s) 1408, 1629, 1716
BstNSI RCATGY 2 cut(s) 1079, 1340
BstPI GGTNACC 2 cut(s) 1196, 1729
BstSCI CCNGG 7 cut(s) 137, 143, 144, 160, 1406, 1627, 1714
BstSFI CTRYAG 1 cut(s) 602
BstSLI GKGCMC 2 cut(s) 900, 1112
BstUI CGCG 3 cut(s) 24, 56, 494
BstV1I GCAGC 4 cut(s) 326, 368, 1555, 1601
BstV2I GAAGAC 3 cut(s) 1078, 1226, 1815
BstX2I RGATCY 3 cut(s) 130, 422, 1485
BstXI CCANNNNNNTGG 2 cut(s) 1026, 1722
BstYI RGATCY 3 cut(s) 130, 422, 1485
BstZ17I GTATAC 1 cut(s) 1039
BstZI CGGCCG 1 cut(s) 39
Bsu36I CCTNAGG 2 cut(s) 1451, 1704
BsuI GTATCC 1 cut(s) 1635
BsuRI GGCC 9 cut(s) 5, 41, 50, 168, 486, 754, 1110, 1406, 1635
BtgI CCRYGG 1 cut(s) 1019
BtrI CACGTC 2 cut(s) 184, 460
BtsCI GGATG 7 cut(s) 156, 358, 706, 706, 740, 1079, 1731
BtsI GCAGTG 2 cut(s) 1344, 1626
BtsIMutI CAGTG 4 cut(s) 594, 715, 1344, 1626
Cac8I GCNNGC 4 cut(s) 215, 344, 484, 1372
CfoI GCGC 5 cut(s) 24, 219, 361, 871, 1573
Cfr10I RCCGGY 4 cut(s) 18, 335, 1156, 1582
Cfr13I GGNCC 8 cut(s) 4, 141, 148, 475, 1108, 1109, 1634, 1700
Cfr9I CCCGGG 1 cut(s) 144
Csp6I GTAC 6 cut(s) 16, 116, 268, 513, 1177, 1355
CspAI ACCGGT 2 cut(s) 1156, 1582
CviAII CATG 3 cut(s) 947, 1076, 1337
CviQI GTAC 6 cut(s) 16, 116, 268, 513, 1177, 1355
DinI GGCGCC 1 cut(s) 870
DrdI GACNNNNNNGTC 1 cut(s) 1801
DriI GACNNNNNGTC 1 cut(s) 31
DseDI GACNNNNNNGTC 1 cut(s) 1801
EaeI YGGCCR 1 cut(s) 39
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 1 cut(s) 624
Eam1105I GACNNNNNGTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 624
EciI GGCGGA 1 cut(s) 1081
EclXI CGGCCG 1 cut(s) 39
Eco130I CCWWGG 2 cut(s) 1650, 1683
Eco147I AGGCCT 1 cut(s) 1406
Eco24I GRGCYC 4 cut(s) 594, 1033, 1112, 1278
Eco47I GGWCC 4 cut(s) 141, 148, 475, 1700
Eco52I CGGCCG 1 cut(s) 39
Eco57I CTGAAG 3 cut(s) 648, 1076, 1202
Eco81I CCTNAGG 2 cut(s) 1451, 1704
Eco88I CYCGRG 1 cut(s) 144
Eco91I GGTNACC 2 cut(s) 1196, 1729
EcoNI CCTNNNNNAGG 1 cut(s) 748
EcoO109I RGGNCCY 4 cut(s) 148, 475, 1109, 1700
EcoO65I GGTNACC 2 cut(s) 1196, 1729
EcoRI GAATTC 1 cut(s) 1612
EcoRII CCWGG 3 cut(s) 1406, 1627, 1714
EcoT14I CCWWGG 2 cut(s) 1650, 1683
EcoT22I ATGCAT 1 cut(s) 1081
EcoT38I GRGCYC 4 cut(s) 594, 1033, 1112, 1278
EgeI GGCGCC 1 cut(s) 870
EheI GGCGCC 1 cut(s) 870
ErhI CCWWGG 2 cut(s) 1650, 1683
FaeI CATG 3 cut(s) 950, 1079, 1340
FaqI GGGAC 2 cut(s) 161, 488
FatI CATG 3 cut(s) 946, 1075, 1336
FauI CCCGC 1 cut(s) 36
FauNDI CATATG 1 cut(s) 1203
FbaI TGATCA 2 cut(s) 1261, 1548
FblI GTMKAC 1 cut(s) 1038
Fnu4HI GCNGC 8 cut(s) 39, 57, 166, 340, 357, 492, 1569, 1590
FokI GGATG 7 cut(s) 143, 365, 693, 713, 727, 1066, 1738
FriOI GRGCYC 4 cut(s) 594, 1033, 1112, 1278
Fsp4HI GCNGC 8 cut(s) 39, 57, 166, 340, 357, 492, 1569, 1590
GlaI GCGC 5 cut(s) 23, 218, 360, 870, 1572
GluI GCNGC 8 cut(s) 39, 57, 166, 340, 357, 492, 1569, 1590
GsuI CTGGAG 1 cut(s) 1017
HaeII RGCGCY 2 cut(s) 362, 872
HaeIII GGCC 9 cut(s) 5, 41, 50, 168, 486, 754, 1110, 1406, 1635
HhaI GCGC 5 cut(s) 24, 219, 361, 871, 1573
Hin1I GRCGYC 2 cut(s) 237, 869
Hin1II CATG 3 cut(s) 950, 1079, 1340
Hin6I GCGC 5 cut(s) 22, 217, 359, 869, 1571
HinP1I GCGC 5 cut(s) 22, 217, 359, 869, 1571
HincII GTYRAC 2 cut(s) 99, 1126
HindII GTYRAC 2 cut(s) 99, 1126
HindIII AAGCTT 1 cut(s) 1742
HinfI GANTC 7 cut(s) 656, 1142, 1167, 1382, 1533, 1764, 1811
HpaI GTTAAC 1 cut(s) 1126
HphI GGTGA 2 cut(s) 544, 1208
Hpy166II GTNNAC 7 cut(s) 16, 99, 189, 559, 715, 1039, 1126
Hpy188III TCNNGA 3 cut(s) 1450, 1530, 1703
Hpy8I GTNNAC 7 cut(s) 16, 99, 189, 559, 715, 1039, 1126
Hpy99I CGWCG 5 cut(s) 56, 176, 239, 242, 1672
HpyAV CCTTC 9 cut(s) 40, 343, 474, 804, 1129, 1178, 1255, 1499, 1656
HpyCH4III ACNGT 3 cut(s) 386, 1359, 1471
HpyCH4IV ACGT 7 cut(s) 183, 237, 459, 561, 1122, 1522, 1670
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 47, 56, 210, 488
HpySE526I ACGT 7 cut(s) 183, 237, 459, 561, 1122, 1522, 1670
Hsp92I GRCGYC 2 cut(s) 237, 869
Hsp92II CATG 3 cut(s) 950, 1079, 1340
HspAI GCGC 5 cut(s) 22, 217, 359, 869, 1571
KasI GGCGCC 1 cut(s) 868
KflI GGGWCCC 1 cut(s) 475
Ksp22I TGATCA 2 cut(s) 1261, 1548
KspAI GTTAAC 1 cut(s) 1126
LmnI GCTCC 6 cut(s) 236, 442, 988, 1036, 1323, 1736
Lsp1109I GCAGC 4 cut(s) 326, 368, 1555, 1601
LweI GCATC 6 cut(s) 343, 749, 1088, 1231, 1383, 1484
MaeII ACGT 7 cut(s) 183, 237, 459, 561, 1122, 1522, 1670
MaeIII GTNAC 7 cut(s) 71, 570, 793, 1159, 1196, 1396, 1729
MbiI CCGCTC 1 cut(s) 231
MboII GAAGA 8 cut(s) 547, 641, 651, 1083, 1208, 1226, 1503, 1820
MfeI CAATTG 1 cut(s) 428
MflI RGATCY 3 cut(s) 130, 422, 1485
MhlI GDGCHC 5 cut(s) 594, 900, 1033, 1112, 1278
Mly113I GGCGCC 1 cut(s) 869
MlyI GAGTC 2 cut(s) 1376, 1805
MmeI TCCRAC 3 cut(s) 108, 158, 258
Mph1103I ATGCAT 1 cut(s) 1081
MseI TTAA 4 cut(s) 315, 327, 614, 1125
MslI CAYNNNNRTG 4 cut(s) 884, 1024, 1269, 1339
MspR9I CCNGG 7 cut(s) 139, 145, 146, 162, 1408, 1629, 1716
MunI CAATTG 1 cut(s) 428
MvaI CCWGG 3 cut(s) 1408, 1629, 1716
MvnI CGCG 3 cut(s) 24, 56, 494
MwoI GCNNNNNNNGC 5 cut(s) 28, 47, 56, 210, 488
NarI GGCGCC 1 cut(s) 869
NciI CCSGG 4 cut(s) 139, 145, 146, 162
NdeI CATATG 1 cut(s) 1203
NlaIII CATG 3 cut(s) 950, 1079, 1340
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 2 cut(s) 1196, 1396
NsiI ATGCAT 1 cut(s) 1081
NspI RCATGY 2 cut(s) 1079, 1340
NspV TTCGAA 1 cut(s) 808
PceI AGGCCT 1 cut(s) 1406
PciI ACATGT 1 cut(s) 1336
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfeI GAWTC 5 cut(s) 656, 1142, 1167, 1533, 1764
PflFI GACNNNGTC 1 cut(s) 152
PinAI ACCGGT 2 cut(s) 1156, 1582
PkrI GCNGC 8 cut(s) 40, 58, 167, 341, 358, 493, 1570, 1591
PleI GAGTC 2 cut(s) 1376, 1805
PluTI GGCGCC 1 cut(s) 872
PpsI GAGTC 2 cut(s) 1376, 1805
Ppu21I YACGTR 2 cut(s) 562, 1523
PpuMI RGGWCCY 3 cut(s) 148, 475, 1700
PscI ACATGT 1 cut(s) 1336
Psp5II RGGWCCY 3 cut(s) 148, 475, 1700
Psp6I CCWGG 3 cut(s) 1406, 1627, 1714
PspEI GGTNACC 2 cut(s) 1196, 1729
PspGI CCWGG 3 cut(s) 1406, 1627, 1714
PspOMI GGGCCC 1 cut(s) 1108
PspPI GGNCC 8 cut(s) 4, 141, 148, 475, 1108, 1109, 1634, 1700
PspPPI RGGWCCY 3 cut(s) 148, 475, 1700
PstI CTGCAG 1 cut(s) 606
PsuI RGATCY 3 cut(s) 130, 422, 1485
PsyI GACNNNGTC 1 cut(s) 152
RsaI GTAC 6 cut(s) 17, 117, 269, 514, 1178, 1356
RsaNI GTAC 6 cut(s) 16, 116, 268, 513, 1177, 1355
RseI CAYNNNNRTG 4 cut(s) 884, 1024, 1269, 1339
SaqAI TTAA 4 cut(s) 315, 327, 614, 1125
SatI GCNGC 8 cut(s) 39, 57, 166, 340, 357, 492, 1569, 1590
Sau96I GGNCC 8 cut(s) 4, 141, 148, 475, 1108, 1109, 1634, 1700
SchI GAGTC 2 cut(s) 1376, 1805
ScrFI CCNGG 7 cut(s) 139, 145, 146, 162, 1408, 1629, 1716
SduI GDGCHC 5 cut(s) 594, 900, 1033, 1112, 1278
SfaNI GCATC 6 cut(s) 343, 749, 1088, 1231, 1383, 1484
SfcI CTRYAG 1 cut(s) 602
SfiI GGCCNNNNNGGCC 1 cut(s) 47
SfoI GGCGCC 1 cut(s) 870
SfuI TTCGAA 1 cut(s) 808
SgrAI CRCCGGYG 1 cut(s) 1582
SinI GGWCC 4 cut(s) 141, 148, 475, 1700
SmaI CCCGGG 1 cut(s) 146
SmiMI CAYNNNNRTG 4 cut(s) 884, 1024, 1269, 1339
SmlI CTYRAG 3 cut(s) 587, 775, 1510
SmoI CTYRAG 3 cut(s) 587, 775, 1510
SseBI AGGCCT 1 cut(s) 1406
SsiI CCGC 7 cut(s) 29, 38, 56, 165, 229, 492, 1066
SspDI GGCGCC 1 cut(s) 868
StuI AGGCCT 1 cut(s) 1406
StyD4I CCNGG 7 cut(s) 137, 143, 144, 160, 1406, 1627, 1714
StyI CCWWGG 2 cut(s) 1650, 1683
TaaI ACNGT 3 cut(s) 386, 1359, 1471
TaiI ACGT 7 cut(s) 186, 240, 462, 564, 1125, 1525, 1673
TaqI TCGA 6 cut(s) 123, 808, 854, 1153, 1762, 1814
TatI WGTACW 2 cut(s) 267, 1354
TauI GCSGC 4 cut(s) 41, 59, 168, 494
TfiI GAWTC 5 cut(s) 656, 1142, 1167, 1533, 1764
Tru1I TTAA 4 cut(s) 315, 327, 614, 1125
Tru9I TTAA 4 cut(s) 315, 327, 614, 1125
TscAI CASTG 4 cut(s) 601, 722, 1351, 1626
TseFI GTSAC 2 cut(s) 1196, 1396
TseI GCWGC 4 cut(s) 339, 356, 1568, 1589
Tsp45I GTSAC 2 cut(s) 1196, 1396
TspDTI ATGAA 5 cut(s) 487, 669, 1148, 1525, 1776
TspGWI ACGGA 3 cut(s) 103, 291, 1160
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 4 cut(s) 601, 722, 1351, 1626
Tth111I GACNNNGTC 1 cut(s) 152
VpaK11BI GGWCC 4 cut(s) 141, 148, 475, 1700
XagI CCTNNNNNAGG 1 cut(s) 748
XapI RAATTY 3 cut(s) 297, 1007, 1612
XceI RCATGY 2 cut(s) 1079, 1340
XmaI CCCGGG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 1038
ZraI GACGTC 1 cut(s) 238
Zsp2I ATGCAT 1 cut(s) 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.