Prupe.4G118300_v2.0.a1

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012288
Physical Location & Seq
Forward (+)
6403278 .. 6406519
3242 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G118300.3

Sequence Viewer

Length: 762 bp
ATGTCATTATTGTCGAAATTGCGGTGCATTACAGTGGATGTTACTGGTACCTTGTTAGCTTACAAGGGGGAGCTTGGTGACTACTATTGCATGGCAGCCAAATCTGTAGGGCTGCCATGCCCTGATTACCAAAGGGTGCATGAGGGCTTCAAATATGCATATAAAGATATGGCCAGCAAGTATCCATGTTTTGGACATGCTGCAAAAATGCCCAACATTGTATGGTGGAAAGCTTGTGTCAGAGACTCCTTTATGAGGGCTGGATATCATTACGATGAGGAGACATTTGAGAAAGTGTTCCGACGCATATATGCATCCTTTGGTTCCTCTGCTCCTTATTCCATCTTTCCTGACTCCCAACCATTTCTGAGATGGGTCCATGAACAAGGTCTTAAAGTTGGAATTATCAGCAATGCGGAATACCGTTATCGAGATGTAATTCTTCCAGCATTGGGTGTCAACCAGGGATCTGATTGGGACTTTGGCGTGTTCTCTGGTATCGAAGGCGTGGAGAAACCAGACCCAAGGATTTATGAGATTGCGCTTGAGAGGGCTGGAAATATGGCCCCGGAAGAGGTTCTGCACATAGGGGACAGCATGCGCAAAGACTATGTGCCAGCAACAAGTATTGGGATGCATGCGTTGTTATTGGATAGGTTTAAGACATCTGATGCTGAGGAGTGGAGGAAATCTGGTGCGGTTGTGCTTCCTGACTTGGAAGCAGCAAAAGAATGGCTTACTTCAGAGAAGTCATCCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase

Protein Analysis

254

Amino Acids

28.62

Weight (kDa)

5.91

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44730 AT5G44730
fragaria_vesca FvH4_3g13030
malus_domestica MD10G1226900.v1.1
prunus_persica Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1
pyrus_communis pycom10g19080
rosa_chinensis RchiOBHm_Chr5g0021221
rosa_laevigata RLG00000032596
rosa_multiflora Rmu_sc0002467.1_g000017 Rmu_sc0003392.1_g000018
rosa_roxburghii Rroxscaffold_1G00057280
rosa_rugosa Rorug05G0065300
rosa_samantha Rh5AG154300 Rh5BG153900 Rh5CG167500 Rh5DG154400
rosa_wichuraiana Rw5G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 602
Acc65I GGTACC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 47
AccB7I CCANNNNNTGG 1 cut(s) 191
AciI CCGC 3 cut(s) 22, 416, 698
AclWI GGATC 1 cut(s) 475
AcoI YGGCCR 1 cut(s) 171
AcuI CTGAAG 1 cut(s) 726
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 4 cut(s) 191, 255, 452, 574
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 1 cut(s) 462
AluBI AGCT 3 cut(s) 59, 73, 233
AluI AGCT 3 cut(s) 59, 73, 233
Alw26I GTCTC 2 cut(s) 237, 275
AlwI GGATC 1 cut(s) 475
AoxI GGCC 2 cut(s) 171, 564
ApeKI GCWGC 4 cut(s) 95, 112, 200, 722
Asp700I GAANNNNTTC 2 cut(s) 296, 576
Asp718I GGTACC 1 cut(s) 47
AspLEI GCGC 2 cut(s) 544, 603
AspS9I GGNCC 2 cut(s) 376, 565
AsuC2I CCSGG 1 cut(s) 569
AsuHPI GGTGA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 376
BalI TGGCCA 1 cut(s) 173
BanI GGYRCC 1 cut(s) 47
BbvCI CCTCAGC 1 cut(s) 675
BbvI GCAGC 4 cut(s) 99, 107, 187, 734
BccI CCATC 2 cut(s) 350, 366
BciT130I CCWGG 1 cut(s) 464
BciVI GTATCC 1 cut(s) 192
BcnI CCSGG 1 cut(s) 569
BcoDI GTCTC 2 cut(s) 237, 275
BfmI CTRYAG 1 cut(s) 105
BfuI GTATCC 1 cut(s) 192
BisI GCNGC 4 cut(s) 96, 113, 201, 723
BlsI GCNGC 4 cut(s) 97, 114, 202, 724
Bme1390I CCNGG 2 cut(s) 464, 569
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 2 cut(s) 376, 565
BmiI GGNNCC 4 cut(s) 49, 325, 377, 567
BmrFI CCNGG 2 cut(s) 464, 569
BmsI GCATC 3 cut(s) 323, 624, 661
Bpu10I CCTNAGC 1 cut(s) 675
BpuEI CTTGAG 1 cut(s) 566
BpuMI CCSGG 1 cut(s) 569
BsaJI CCNNGG 3 cut(s) 463, 524, 567
Bsc4I CCNNNNNNNGG 4 cut(s) 191, 255, 452, 574
Bse1I ACTGG 1 cut(s) 49
Bse3DI GCAATG 1 cut(s) 418
BseBI CCWGG 1 cut(s) 464
BseDI CCNNGG 3 cut(s) 463, 524, 567
BseGI GGATG 4 cut(s) 43, 314, 639, 752
BseLI CCNNNNNNNGG 4 cut(s) 191, 255, 452, 574
BseMI GCAATG 1 cut(s) 418
BseMII CTCAG 2 cut(s) 359, 666
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 2 cut(s) 293, 692
BseXI GCAGC 4 cut(s) 99, 107, 187, 734
BsgI GTGCAG 1 cut(s) 566
BshFI GGCC 2 cut(s) 173, 566
BshNI GGYRCC 1 cut(s) 47
BsiSI CCGG 1 cut(s) 569
BslFI GGGAC 2 cut(s) 491, 605
BslI CCNNNNNNNGG 4 cut(s) 191, 255, 452, 574
BsmAI GTCTC 2 cut(s) 237, 275
BsmFI GGGAC 2 cut(s) 491, 605
BsnI GGCC 2 cut(s) 173, 566
Bsp143I GATC 1 cut(s) 467
BspACI CCGC 3 cut(s) 22, 416, 698
BspANI GGCC 2 cut(s) 173, 566
BspCNI CTCAG 2 cut(s) 360, 667
BspLI GGNNCC 4 cut(s) 49, 325, 377, 567
BspPI GGATC 1 cut(s) 475
BspT107I GGYRCC 1 cut(s) 47
BsrDI GCAATG 1 cut(s) 418
BsrI ACTGG 1 cut(s) 49
BssECI CCNNGG 3 cut(s) 463, 524, 567
BssMI GATC 1 cut(s) 467
BssT1I CCWWGG 1 cut(s) 524
Bst2UI CCWGG 1 cut(s) 464
Bst4CI ACNGT 2 cut(s) 34, 425
Bst6I CTCTTC 1 cut(s) 567
BstC8I GCNNGC 4 cut(s) 175, 599, 618, 639
BstDEI CTNAG 2 cut(s) 368, 675
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 4 cut(s) 43, 314, 639, 752
BstHHI GCGC 2 cut(s) 544, 603
BstKTI GATC 1 cut(s) 470
BstMAI GTCTC 2 cut(s) 237, 275
BstMBI GATC 1 cut(s) 467
BstNI CCWGG 1 cut(s) 464
BstNSI RCATGY 3 cut(s) 200, 601, 641
BstSCI CCNGG 2 cut(s) 462, 567
BstSFI CTRYAG 1 cut(s) 105
BstV1I GCAGC 4 cut(s) 99, 107, 187, 734
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
BsuI GTATCC 1 cut(s) 192
BsuRI GGCC 2 cut(s) 173, 566
BtsCI GGATG 4 cut(s) 43, 314, 639, 752
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 4 cut(s) 175, 599, 618, 639
CfoI GCGC 2 cut(s) 544, 603
Cfr13I GGNCC 2 cut(s) 376, 565
CseI GACGC 1 cut(s) 312
Csp6I GTAC 1 cut(s) 48
CviAII CATG 8 cut(s) 91, 117, 140, 186, 197, 380, 598, 638
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 2 cut(s) 368, 675
DpnI GATC 1 cut(s) 469
DpnII GATC 1 cut(s) 467
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Eco130I CCWWGG 1 cut(s) 524
Eco32I GATATC 1 cut(s) 266
Eco47I GGWCC 1 cut(s) 376
Eco57I CTGAAG 1 cut(s) 726
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 462
EcoRV GATATC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 524
EcoT22I ATGCAT 3 cut(s) 160, 316, 639
ErhI CCWWGG 1 cut(s) 524
FaeI CATG 8 cut(s) 94, 120, 143, 189, 200, 383, 601, 641
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FaqI GGGAC 2 cut(s) 491, 605
FatI CATG 8 cut(s) 90, 116, 139, 185, 196, 379, 597, 637
Fnu4HI GCNGC 4 cut(s) 96, 113, 201, 723
FokI GGATG 4 cut(s) 50, 301, 646, 739
Fsp4HI GCNGC 4 cut(s) 96, 113, 201, 723
FspI TGCGCA 1 cut(s) 602
GlaI GCGC 2 cut(s) 543, 602
GluI GCNGC 4 cut(s) 96, 113, 201, 723
HaeIII GGCC 2 cut(s) 173, 566
HapII CCGG 1 cut(s) 569
HgaI GACGC 1 cut(s) 312
HhaI GCGC 2 cut(s) 544, 603
Hin1II CATG 8 cut(s) 94, 120, 143, 189, 200, 383, 601, 641
Hin6I GCGC 2 cut(s) 542, 601
HinP1I GCGC 2 cut(s) 542, 601
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 231
HinfI GANTC 2 cut(s) 245, 353
HpaII CCGG 1 cut(s) 569
HphI GGTGA 1 cut(s) 89
Hpy166II GTNNAC 1 cut(s) 460
Hpy188I TCNGA 6 cut(s) 242, 302, 369, 472, 670, 745
Hpy188III TCNNGA 3 cut(s) 350, 431, 710
Hpy8I GTNNAC 1 cut(s) 460
Hpy99I CGWCG 1 cut(s) 306
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 2 cut(s) 34, 425
HpyCH4V TGCA 8 cut(s) 27, 90, 139, 158, 203, 314, 583, 637
HpyF3I CTNAG 2 cut(s) 368, 675
Hsp92II CATG 8 cut(s) 94, 120, 143, 189, 200, 383, 601, 641
HspAI GCGC 2 cut(s) 542, 601
KpnI GGTACC 1 cut(s) 51
Kzo9I GATC 1 cut(s) 467
LmnI GCTCC 2 cut(s) 70, 337
Lsp1109I GCAGC 4 cut(s) 99, 107, 187, 734
LweI GCATC 3 cut(s) 323, 624, 661
MaeIII GTNAC 2 cut(s) 40, 77
MalI GATC 1 cut(s) 469
MboI GATC 1 cut(s) 467
MboII GAAGA 2 cut(s) 434, 584
MflI RGATCY 1 cut(s) 467
MlsI TGGCCA 1 cut(s) 173
MluCI AATT 3 cut(s) 17, 402, 438
MluNI TGGCCA 1 cut(s) 173
MlyI GAGTC 2 cut(s) 239, 347
MmeI TCCRAC 2 cut(s) 325, 379
MnlI CCTC 8 cut(s) 136, 249, 271, 337, 543, 568, 670, 678
Mox20I TGGCCA 1 cut(s) 173
Mph1103I ATGCAT 3 cut(s) 160, 316, 639
MroXI GAANNNNTTC 2 cut(s) 296, 576
MscI TGGCCA 1 cut(s) 173
MseI TTAA 2 cut(s) 393, 660
MslI CAYNNNNRTG 2 cut(s) 32, 273
Msp20I TGGCCA 1 cut(s) 173
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 2 cut(s) 464, 569
MvaI CCWGG 1 cut(s) 464
NciI CCSGG 1 cut(s) 569
NdeII GATC 1 cut(s) 467
NlaIII CATG 8 cut(s) 94, 120, 143, 189, 200, 383, 601, 641
NlaIV GGNNCC 4 cut(s) 49, 325, 377, 567
NmuCI GTSAC 1 cut(s) 77
NsbI TGCGCA 1 cut(s) 602
NsiI ATGCAT 3 cut(s) 160, 316, 639
NspI RCATGY 3 cut(s) 200, 601, 641
PaeI GCATGC 2 cut(s) 601, 641
PdmI GAANNNNTTC 2 cut(s) 296, 576
PflMI CCANNNNNTGG 1 cut(s) 191
PkrI GCNGC 4 cut(s) 97, 114, 202, 724
PleI GAGTC 2 cut(s) 239, 347
PpsI GAGTC 2 cut(s) 239, 347
Psp6I CCWGG 1 cut(s) 462
PspGI CCWGG 1 cut(s) 462
PspN4I GGNNCC 4 cut(s) 49, 325, 377, 567
PspPI GGNCC 2 cut(s) 376, 565
PsuI RGATCY 1 cut(s) 467
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
RseI CAYNNNNRTG 2 cut(s) 32, 273
SaqAI TTAA 2 cut(s) 393, 660
SatI GCNGC 4 cut(s) 96, 113, 201, 723
Sau3AI GATC 1 cut(s) 467
Sau96I GGNCC 2 cut(s) 376, 565
SchI GAGTC 2 cut(s) 239, 347
ScrFI CCNGG 2 cut(s) 464, 569
SetI ASST 7 cut(s) 53, 61, 75, 235, 391, 579, 659
SfaNI GCATC 3 cut(s) 323, 624, 661
SfcI CTRYAG 1 cut(s) 105
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 2 cut(s) 32, 273
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
SphI GCATGC 2 cut(s) 601, 641
Sse9I AATT 3 cut(s) 17, 402, 438
SsiI CCGC 3 cut(s) 22, 416, 698
StyD4I CCNGG 2 cut(s) 462, 567
StyI CCWWGG 1 cut(s) 524
TaaI ACNGT 2 cut(s) 34, 425
TaqI TCGA 3 cut(s) 14, 430, 501
TasI AATT 3 cut(s) 17, 402, 438
Tru1I TTAA 2 cut(s) 393, 660
Tru9I TTAA 2 cut(s) 393, 660
TscAI CASTG 1 cut(s) 39
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 4 cut(s) 95, 112, 200, 722
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 1 cut(s) 396
TspRI CASTG 1 cut(s) 39
Van91I CCANNNNNTGG 1 cut(s) 191
VpaK11BI GGWCC 1 cut(s) 376
XagI CCTNNNNNAGG 1 cut(s) 253
XceI RCATGY 3 cut(s) 200, 601, 641
XcmI CCANNNNNNNNNTGG 1 cut(s) 369
XmnI GAANNNNTTC 2 cut(s) 296, 576
Zsp2I ATGCAT 3 cut(s) 160, 316, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.