RLG00000032596

Haloacid dehalogenase-like hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012288
Physical Location & Seq
Forward (+)
16892576 .. 16896162
3587 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032596

Sequence Viewer

Length: 819 bp
ATGAGTGAAGGTGCTTTCAGCATCTGGGGATTGTTGGAACTGCTGAAAAGTTTTACAATGTCATTGTTGTCAAAGTTGCGGTGCATCACGGTGGATGTTACTGGTACATTGATAGCTTACAAGGGGGAGCTTGGTGACTACTATTGCATGGCAGCCAAATCCGTAGGGCAGCCATGCCCCGACTACAAAAGGATGCATGAGGGCTTCAAAAATGCTTATAAAGACATGGCCAGCAAATATCCTTGTTTTGGGCATGCTGCCAAAATGCCCAACATTGAATGGTGGAAAACTTGTGTCAGAGATTCCTTTATCAGAGCTGGATATCATTATGATGAGGAGACATTTGAGAAAGTGTTTCGACGCATATATGCATCCTTTGGTTCTGCTGCTCCTTATAGCATCTTTCCCGATTCCCAACCATTTATGAGATGGGTCCGTGAACAAGGTCTTATAGTTGGAATTGTTAGCAATGCAGAATACCGGTATCAGGATGTTATTCTTCCAGCCTTGGGTTTGAACCAGGGATCTGAATGGGACTTCGGTGTGTTCTCTGGTCTCGAAGGTGTGGAGAAACCAGACCCAAGGATTTATGAGATTGCGCTTAAGAGGGCTGGCAACCTAAAACCAGAAGAGGTTCTGCACATAGGGGACAGCATGCGCAAAGACTACGTGCCAGCAACAAGTATTGGGATGCATGCACTGTTATTGGACAGGTTTAAGACATCTGATGCTGAGGAATGGAGGAAATCTGGTGCGGTTGTGCTCCCTGATTTGAAAGCAGCAAAAGAATGGATTACTTCAGAGAAGTCAGCATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase

Protein Analysis

273

Amino Acids

30.78

Weight (kDa)

6.54

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 27 - 223 3.5e-24 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 101 - 233 2.6e-09 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 191 - 260 3e-08 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012288)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44730 AT5G44730
fragaria_vesca FvH4_3g13030
malus_domestica MD10G1226900.v1.1
prunus_persica Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1 Prupe.4G118300_v2.0.a1
pyrus_communis pycom10g19080
rosa_chinensis RchiOBHm_Chr5g0021221
rosa_laevigata RLG00000032596
rosa_multiflora Rmu_sc0002467.1_g000017 Rmu_sc0003392.1_g000018
rosa_roxburghii Rroxscaffold_1G00057280
rosa_rugosa Rorug05G0065300
rosa_samantha Rh5AG154300 Rh5BG153900 Rh5CG167500 Rh5DG154400
rosa_wichuraiana Rw5G013750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 219
Acc16I TGCGCA 1 cut(s) 659
AciI CCGC 2 cut(s) 79, 755
AclWI GGATC 1 cut(s) 532
AcoI YGGCCR 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 783
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 3 cut(s) 248, 487, 509
AflII CTTAAG 1 cut(s) 602
AgeI ACCGGT 1 cut(s) 480
AgsI TTSAA 4 cut(s) 208, 278, 517, 775
AjnI CCWGG 1 cut(s) 519
AluBI AGCT 3 cut(s) 116, 130, 317
AluI AGCT 3 cut(s) 116, 130, 317
Alw21I GWGCWC 1 cut(s) 765
Alw26I GTCTC 2 cut(s) 332, 560
AlwI GGATC 1 cut(s) 532
AlwNI CAGNNNCTG 1 cut(s) 24
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 5 cut(s) 152, 169, 257, 386, 779
AsiGI ACCGGT 1 cut(s) 480
Asp700I GAANNNNTTC 1 cut(s) 633
AspLEI GCGC 2 cut(s) 601, 660
AspS9I GGNCC 1 cut(s) 433
AsuHPI GGTGA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 433
BalI TGGCCA 1 cut(s) 230
Bbv12I GWGCWC 1 cut(s) 765
BbvCI CCTCAGC 1 cut(s) 732
BbvI GCAGC 5 cut(s) 164, 181, 244, 373, 791
BccI CCATC 1 cut(s) 423
BciT130I CCWGG 1 cut(s) 521
BcoDI GTCTC 2 cut(s) 332, 560
BfrI CTTAAG 1 cut(s) 602
BisI GCNGC 5 cut(s) 153, 170, 258, 387, 780
BlsI GCNGC 5 cut(s) 154, 171, 259, 388, 781
Bme1390I CCNGG 1 cut(s) 521
Bme18I GGWCC 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 433
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 7 cut(s) 30, 93, 183, 380, 408, 681, 718
Bpu10I CCTNAGC 1 cut(s) 732
BsaAI YACGTR 1 cut(s) 670
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 3 cut(s) 507, 520, 581
BsaWI WCCGGW 1 cut(s) 480
Bsc4I CCNNNNNNNGG 3 cut(s) 248, 487, 509
Bse118I RCCGGY 1 cut(s) 480
Bse1I ACTGG 1 cut(s) 106
Bse3DI GCAATG 1 cut(s) 475
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 3 cut(s) 507, 520, 581
BseGI GGATG 5 cut(s) 100, 198, 371, 496, 696
BseLI CCNNNNNNNGG 3 cut(s) 248, 487, 509
BseMI GCAATG 1 cut(s) 475
BseMII CTCAG 1 cut(s) 723
BseNI ACTGG 1 cut(s) 106
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 5 cut(s) 164, 181, 244, 373, 791
BsgI GTGCAG 1 cut(s) 623
BshFI GGCC 1 cut(s) 230
BshTI ACCGGT 1 cut(s) 480
BsiHKAI GWGCWC 1 cut(s) 765
BsiSI CCGG 1 cut(s) 481
BslFI GGGAC 2 cut(s) 548, 662
BslI CCNNNNNNNGG 3 cut(s) 248, 487, 509
BsmAI GTCTC 2 cut(s) 332, 560
BsmFI GGGAC 2 cut(s) 548, 662
BsnI GGCC 1 cut(s) 230
Bso31I GGTCTC 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 765
Bsp143I GATC 1 cut(s) 524
BspACI CCGC 2 cut(s) 79, 755
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 1 cut(s) 724
BspLI GGNNCC 1 cut(s) 434
BspPI GGATC 1 cut(s) 532
BspTI CTTAAG 1 cut(s) 602
BspTNI GGTCTC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 475
BsrFI RCCGGY 1 cut(s) 480
BsrI ACTGG 1 cut(s) 106
BssAI RCCGGY 1 cut(s) 480
BssECI CCNNGG 3 cut(s) 507, 520, 581
BssMI GATC 1 cut(s) 524
BssT1I CCWWGG 2 cut(s) 507, 581
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 2 cut(s) 91, 702
Bst6I CTCTTC 1 cut(s) 624
BstAFI CTTAAG 1 cut(s) 602
BstBAI YACGTR 1 cut(s) 670
BstC8I GCNNGC 7 cut(s) 232, 255, 613, 656, 675, 696, 814
BstDEI CTNAG 1 cut(s) 732
BstF5I GGATG 5 cut(s) 100, 198, 371, 496, 696
BstHHI GCGC 2 cut(s) 601, 660
BstKTI GATC 1 cut(s) 527
BstMAI GTCTC 2 cut(s) 332, 560
BstMBI GATC 1 cut(s) 524
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 4 cut(s) 257, 658, 698, 816
BstSCI CCNGG 1 cut(s) 519
BstV1I GCAGC 5 cut(s) 164, 181, 244, 373, 791
BstX2I RGATCY 1 cut(s) 524
BstYI RGATCY 1 cut(s) 524
BsuRI GGCC 1 cut(s) 230
BtsCI GGATG 5 cut(s) 100, 198, 371, 496, 696
BtsIMutI CAGTG 1 cut(s) 698
Cac8I GCNNGC 7 cut(s) 232, 255, 613, 656, 675, 696, 814
CaiI CAGNNNCTG 1 cut(s) 24
CfoI GCGC 2 cut(s) 601, 660
Cfr10I RCCGGY 1 cut(s) 480
Cfr13I GGNCC 1 cut(s) 433
CseI GACGC 1 cut(s) 369
Csp6I GTAC 1 cut(s) 105
CspAI ACCGGT 1 cut(s) 480
CviAII CATG 8 cut(s) 148, 174, 197, 226, 254, 655, 695, 813
CviJI RGCY 9 cut(s) 116, 130, 155, 172, 204, 230, 317, 506, 611
CviKI_1 RGCY 9 cut(s) 116, 130, 155, 172, 204, 230, 317, 506, 611
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 1 cut(s) 732
DpnI GATC 1 cut(s) 526
DpnII GATC 1 cut(s) 524
EaeI YGGCCR 1 cut(s) 228
Eam1104I CTCTTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 624
Eco130I CCWWGG 2 cut(s) 507, 581
Eco31I GGTCTC 1 cut(s) 560
Eco32I GATATC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 783
EcoRII CCWGG 1 cut(s) 519
EcoRV GATATC 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 507, 581
EcoT22I ATGCAT 3 cut(s) 198, 373, 696
ErhI CCWWGG 2 cut(s) 507, 581
FaeI CATG 8 cut(s) 151, 177, 200, 229, 257, 658, 698, 816
FaqI GGGAC 2 cut(s) 548, 662
FatI CATG 8 cut(s) 147, 173, 196, 225, 253, 654, 694, 812
Fnu4HI GCNGC 5 cut(s) 153, 170, 258, 387, 780
FokI GGATG 5 cut(s) 107, 205, 358, 503, 703
Fsp4HI GCNGC 5 cut(s) 153, 170, 258, 387, 780
FspI TGCGCA 1 cut(s) 659
GlaI GCGC 2 cut(s) 600, 659
GluI GCNGC 5 cut(s) 153, 170, 258, 387, 780
HaeIII GGCC 1 cut(s) 230
HapII CCGG 1 cut(s) 481
HgaI GACGC 1 cut(s) 369
HhaI GCGC 2 cut(s) 601, 660
Hin1II CATG 8 cut(s) 151, 177, 200, 229, 257, 658, 698, 816
Hin6I GCGC 2 cut(s) 599, 658
HinP1I GCGC 2 cut(s) 599, 658
HinfI GANTC 2 cut(s) 302, 410
HpaII CCGG 1 cut(s) 481
HphI GGTGA 1 cut(s) 146
Hpy166II GTNNAC 1 cut(s) 440
Hpy188I TCNGA 5 cut(s) 299, 314, 529, 727, 802
Hpy188III TCNNGA 3 cut(s) 407, 488, 557
Hpy8I GTNNAC 1 cut(s) 440
Hpy99I CGWCG 1 cut(s) 363
HpyAV CCTTC 1 cut(s) 554
HpyCH4III ACNGT 2 cut(s) 91, 702
HpyCH4IV ACGT 1 cut(s) 669
HpyCH4V TGCA 8 cut(s) 84, 147, 196, 371, 473, 640, 694, 698
HpyF3I CTNAG 1 cut(s) 732
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 8 cut(s) 151, 177, 200, 229, 257, 658, 698, 816
HspAI GCGC 2 cut(s) 599, 658
Kzo9I GATC 1 cut(s) 524
LmnI GCTCC 3 cut(s) 127, 394, 768
Lsp1109I GCAGC 5 cut(s) 164, 181, 244, 373, 791
LweI GCATC 7 cut(s) 30, 93, 183, 380, 408, 681, 718
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 2 cut(s) 97, 134
MalI GATC 1 cut(s) 526
MboI GATC 1 cut(s) 524
MboII GAAGA 2 cut(s) 491, 641
MflI RGATCY 1 cut(s) 524
MhlI GDGCHC 1 cut(s) 765
MlsI TGGCCA 1 cut(s) 230
MluCI AATT 1 cut(s) 459
MluNI TGGCCA 1 cut(s) 230
MmeI TCCRAC 2 cut(s) 15, 436
MnlI CCTC 6 cut(s) 193, 328, 600, 625, 727, 735
Mox20I TGGCCA 1 cut(s) 230
Mph1103I ATGCAT 3 cut(s) 198, 373, 696
MroXI GAANNNNTTC 1 cut(s) 633
MscI TGGCCA 1 cut(s) 230
MseI TTAA 2 cut(s) 603, 717
MslI CAYNNNNRTG 2 cut(s) 89, 330
Msp20I TGGCCA 1 cut(s) 230
MspCI CTTAAG 1 cut(s) 602
MspI CCGG 1 cut(s) 481
MspR9I CCNGG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 521
NdeII GATC 1 cut(s) 524
NlaIII CATG 8 cut(s) 151, 177, 200, 229, 257, 658, 698, 816
NlaIV GGNNCC 1 cut(s) 434
NmuCI GTSAC 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 659
NsiI ATGCAT 3 cut(s) 198, 373, 696
NspI RCATGY 4 cut(s) 257, 658, 698, 816
PaeI GCATGC 4 cut(s) 257, 658, 698, 816
PdmI GAANNNNTTC 1 cut(s) 633
PfeI GAWTC 2 cut(s) 302, 410
PinAI ACCGGT 1 cut(s) 480
PkrI GCNGC 5 cut(s) 154, 171, 259, 388, 781
Ppu21I YACGTR 1 cut(s) 670
PsiI TTATAA 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 434
PspPI GGNCC 1 cut(s) 433
PstNI CAGNNNCTG 1 cut(s) 24
PsuI RGATCY 1 cut(s) 524
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
RseI CAYNNNNRTG 2 cut(s) 89, 330
SaqAI TTAA 2 cut(s) 603, 717
SatI GCNGC 5 cut(s) 153, 170, 258, 387, 780
Sau3AI GATC 1 cut(s) 524
Sau96I GGNCC 1 cut(s) 433
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 1 cut(s) 765
SfaNI GCATC 7 cut(s) 30, 93, 183, 380, 408, 681, 718
SinI GGWCC 1 cut(s) 433
SmiMI CAYNNNNRTG 2 cut(s) 89, 330
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SphI GCATGC 4 cut(s) 257, 658, 698, 816
Sse9I AATT 1 cut(s) 459
SsiI CCGC 2 cut(s) 79, 755
StyD4I CCNGG 1 cut(s) 519
StyI CCWWGG 2 cut(s) 507, 581
TaaI ACNGT 2 cut(s) 91, 702
TaiI ACGT 1 cut(s) 672
TaqI TCGA 2 cut(s) 358, 558
TasI AATT 1 cut(s) 459
TfiI GAWTC 2 cut(s) 302, 410
Tru1I TTAA 2 cut(s) 603, 717
Tru9I TTAA 2 cut(s) 603, 717
TscAI CASTG 1 cut(s) 705
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 5 cut(s) 152, 169, 257, 386, 779
Tsp45I GTSAC 1 cut(s) 134
TspGWI ACGGA 2 cut(s) 151, 425
TspRI CASTG 1 cut(s) 705
Vha464I CTTAAG 1 cut(s) 602
VpaK11BI GGWCC 1 cut(s) 433
XceI RCATGY 4 cut(s) 257, 658, 698, 816
XcmI CCANNNNNNNNNTGG 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 633
Zsp2I ATGCAT 3 cut(s) 198, 373, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.