AT5G46560

Man1-Src1p-C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011342
Physical Location & Seq
Forward (+)
18887987 .. 18890517
2531 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46560.1

Sequence Viewer

Length: 1164 bp
ATGGATTCGATACCACGGAAGCGACCCAAATCGGAAACCAGAACGGGCAGAACCCCTAAATCTTCTTCTTCTTCTTCTCCGATCAGATCAATGCTCGAGCCACCACAAAGCTTATTTCCTTCCAAGGGAGAGTTCTTCACACTATTGAAGGTTTTGCTTGTCGCTTGTGCTGTAGCTTTTACCTGCAATTTCCTCTCTAAGTCTCTGAGCTCAAACCCTTCTAAATCTTTCTGCGATAGTAACTTTAATCCCATCGATTCAGACTTGGATATTTGTGAACCTTGTCCAATTAATGGAGAATGTTACCAAGGGAAGTTGCAATGTAATCTTGGATACAAAAATCAAAGGAATTTATGTGTAGAAGATGGAGAAATCAATGAATCAACTAAGAAATTGGTTGGGTATTTTGAGAGAAAAGTTTGTGAATCATATGCTCATAATGAATGTTATGGTACGGGAACCATTTGGGTTCCAGAAAATGATGTTTGGACAGAACTACGTAGTAACAGTTTCTTGAGTAACTTAGACGAGTCTGCTTACAATTTTTTGAAAGGAAAGGCTGTTGAAGGTGTCACCGAGCTACTAGAGAAAAGGACTAATTCTAATGGGATTGACGAGTTGAAGTGCCCTGAATCCGTAGCCAAAAGTTACAAACCTTTGACCTGTCGCTTGCATCAATGGATCTTGCGGCATATCTTAATCATCTCGTCGTCCTGTGCAATGCTAGTGGGCAGCGCAATGTTGCGTAGGAGAATTCAACGTAAGCAATGTTTTTCAAGAAGAGTGGAAGAGTTATACGACCAGGTCTGTGACTTCCTGGAAGAGAATGCAGTGGCATCAAATTCTGCAGAAACTAGTAACTGCGAGCCTTGGGTTATTGCATCTTGGTTACGTGATTATCTACTCTTGCCTAGGGAAAGAAGAGACCCTCTGTTATGGACTAAGGTTGAGGAGTTAATAAAAGAGGATTCACGTATAGACCGATATGAAAAACTCCTCAAAGGTGAAAAAAAAGTTGTATGGGAATGGCAAGTGGAAGGTTCACTTAGTTTATCCAAGTTGAAGAAACAGAGAGAGACGCAGAAGAAAGTCAGAAAAAGTATCGACTCAAGCACAAGCTTGCAAGAGTATTACAACAGACGAATTGCCGAGACAAGTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MSC

Protein Analysis

387

Amino Acids

44.39

Weight (kDa)

8.49

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MSC PF09402 74 - 343 1.1e-17 Man1-Src1p-C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46560
fragaria_vesca FvH4_5g21210 FvH4_5g21210 FvH4_5g21210 FvH4_5g21210
malus_domestica MD06G1040000.v1.1
prunus_persica Prupe.5G046100_v2.0.a1 Prupe.5G046100_v2.0.a1
pyrus_communis pycom06g03340
rosa_chinensis RchiOBHm_Chr7g0208351
rosa_laevigata RLG00000003220
rosa_multiflora Rmu_sc0006741.1_g000001 Rmu_ssc0000063.1_g000001
rosa_roxburghii Rroxscaffold_3G00250530
rosa_rugosa Rorug07G0103800
rosa_samantha Rh7AG238200 Rh7BG233700 Rh7CG253700 Rh7DG243900
rosa_wichuraiana Rw7G020300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 293
AciI CCGC 1 cut(s) 688
AclWI GGATC 1 cut(s) 689
AcsI RAATTY 3 cut(s) 349, 753, 841
AfaI GTAC 1 cut(s) 454
AfiI CCNNNNNNNGG 2 cut(s) 125, 293
AgsI TTSAA 7 cut(s) 148, 550, 566, 622, 758, 777, 1063
AhlI ACTAGT 1 cut(s) 854
AjnI CCWGG 2 cut(s) 801, 816
AjuI GAANNNNNNNTTGG 2 cut(s) 116, 148
AluBI AGCT 5 cut(s) 111, 176, 210, 580, 1119
AluI AGCT 5 cut(s) 111, 176, 210, 580, 1119
Alw21I GWGCWC 1 cut(s) 212
Alw26I GTCTC 4 cut(s) 207, 918, 1070, 1145
AlwI GGATC 1 cut(s) 689
Ama87I CYCGRG 1 cut(s) 95
ApeKI GCWGC 1 cut(s) 732
ApoI RAATTY 3 cut(s) 349, 753, 841
AseI ATTAAT 1 cut(s) 291
AspA2I CCTAGG 1 cut(s) 911
AspLEI GCGC 1 cut(s) 737
AsuHPI GGTGA 2 cut(s) 565, 1016
AvaI CYCGRG 1 cut(s) 95
AvrII CCTAGG 1 cut(s) 911
BaeGI GKGCMC 1 cut(s) 629
BanII GRGCYC 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 212
BbvI GCAGC 1 cut(s) 744
BccI CCATC 2 cut(s) 260, 359
BciT130I CCWGG 2 cut(s) 803, 818
BciVI GTATCC 1 cut(s) 326
BcoDI GTCTC 4 cut(s) 207, 918, 1070, 1145
BcuI ACTAGT 1 cut(s) 854
BfaI CTAG 4 cut(s) 584, 725, 855, 912
BfmI CTRYAG 2 cut(s) 171, 846
BfuAI ACCTGC 1 cut(s) 191
BfuI GTATCC 1 cut(s) 326
BisI GCNGC 2 cut(s) 689, 733
BlnI CCTAGG 1 cut(s) 911
BlsI GCNGC 2 cut(s) 690, 734
Bme1390I CCNGG 2 cut(s) 803, 818
BmeT110I CYCGRG 1 cut(s) 95
BmiI GGNNCC 2 cut(s) 460, 471
BmrFI CCNGG 2 cut(s) 803, 818
BmsI GCATC 3 cut(s) 682, 845, 890
BpuEI CTTGAG 2 cut(s) 535, 1093
Bsa29I ATCGAT 1 cut(s) 255
BsaAI YACGTR 3 cut(s) 500, 893, 974
BsaI GGTCTC 1 cut(s) 918
BsaJI CCNNGG 5 cut(s) 14, 123, 307, 869, 911
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 293
Bse3DI GCAATG 4 cut(s) 326, 726, 744, 773
BseBI CCWGG 2 cut(s) 803, 818
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 5 cut(s) 14, 123, 307, 869, 911
BseLI CCNNNNNNNGG 2 cut(s) 125, 293
BseMI GCAATG 4 cut(s) 326, 726, 744, 773
BseMII CTCAG 1 cut(s) 197
BseRI GAGGAG 2 cut(s) 965, 986
BseSI GKGCMC 1 cut(s) 629
BseXI GCAGC 1 cut(s) 744
BshVI ATCGAT 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 212
BsiHKCI CYCGRG 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 125, 293
BsmAI GTCTC 4 cut(s) 207, 918, 1070, 1145
BsmBI CGTCTC 1 cut(s) 1070
BsmI GAATGC 1 cut(s) 832
Bso31I GGTCTC 1 cut(s) 918
BsoBI CYCGRG 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 212, 629
Bsp143I GATC 3 cut(s) 81, 86, 681
BspACI CCGC 1 cut(s) 688
BspCNI CTCAG 1 cut(s) 198
BspDI ATCGAT 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 460, 471
BspMAI CTGCAG 1 cut(s) 850
BspMI ACCTGC 1 cut(s) 191
BspPI GGATC 1 cut(s) 689
BspTNI GGTCTC 1 cut(s) 918
BsrDI GCAATG 4 cut(s) 326, 726, 744, 773
BssECI CCNNGG 5 cut(s) 14, 123, 307, 869, 911
BssMI GATC 3 cut(s) 81, 86, 681
BssT1I CCWWGG 4 cut(s) 123, 307, 869, 911
Bst2UI CCWGG 2 cut(s) 803, 818
Bst4CI ACNGT 1 cut(s) 509
Bst6I CTCTTC 4 cut(s) 775, 783, 816, 916
BstBAI YACGTR 3 cut(s) 500, 893, 974
BstC8I GCNNGC 3 cut(s) 671, 866, 1121
BstDEI CTNAG 7 cut(s) 198, 206, 387, 523, 942, 1046, 1161
BstDSI CCRYGG 1 cut(s) 14
BstHHI GCGC 1 cut(s) 737
BstKTI GATC 3 cut(s) 84, 89, 684
BstMAI GTCTC 4 cut(s) 207, 918, 1070, 1145
BstMBI GATC 3 cut(s) 81, 86, 681
BstNI CCWGG 2 cut(s) 803, 818
BstSCI CCNGG 2 cut(s) 801, 816
BstSFI CTRYAG 2 cut(s) 171, 846
BstSLI GKGCMC 1 cut(s) 629
BstSNI TACGTA 1 cut(s) 500
BstV1I GCAGC 1 cut(s) 744
BstX2I RGATCY 1 cut(s) 681
BstYI RGATCY 1 cut(s) 681
Bsu15I ATCGAT 1 cut(s) 255
BsuI GTATCC 1 cut(s) 326
BsuTUI ATCGAT 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 837
BtsIMutI CAGTG 1 cut(s) 837
BveI ACCTGC 1 cut(s) 191
Cac8I GCNNGC 3 cut(s) 671, 866, 1121
CfoI GCGC 1 cut(s) 737
ClaI ATCGAT 1 cut(s) 255
CseI GACGC 1 cut(s) 1087
CsiI ACCWGGT 1 cut(s) 801
Csp6I GTAC 1 cut(s) 453
CspCI CAANNNNNGTGG 4 cut(s) 708, 743, 765, 800
CviJI RGCY 9 cut(s) 100, 111, 176, 210, 560, 580, 641, 868, 1119
CviKI_1 RGCY 9 cut(s) 100, 111, 176, 210, 560, 580, 641, 868, 1119
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 7 cut(s) 198, 206, 387, 523, 942, 1046, 1161
DpnI GATC 3 cut(s) 83, 88, 683
DpnII GATC 3 cut(s) 81, 86, 681
Eam1104I CTCTTC 4 cut(s) 775, 783, 816, 916
EarI CTCTTC 4 cut(s) 775, 783, 816, 916
Ecl136II GAGCTC 1 cut(s) 210
Eco105I TACGTA 1 cut(s) 500
Eco130I CCWWGG 4 cut(s) 123, 307, 869, 911
Eco24I GRGCYC 1 cut(s) 212
Eco31I GGTCTC 1 cut(s) 918
Eco53kI GAGCTC 1 cut(s) 210
Eco88I CYCGRG 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 210
EcoRI GAATTC 1 cut(s) 753
EcoRII CCWGG 2 cut(s) 801, 816
EcoT14I CCWWGG 4 cut(s) 123, 307, 869, 911
EcoT38I GRGCYC 1 cut(s) 212
ErhI CCWWGG 4 cut(s) 123, 307, 869, 911
Esp3I CGTCTC 1 cut(s) 1070
FalI AAGNNNNNCTT 2 cut(s) 1029, 1061
FauNDI CATATG 1 cut(s) 430
Fnu4HI GCNGC 2 cut(s) 689, 733
FriOI GRGCYC 1 cut(s) 212
Fsp4HI GCNGC 2 cut(s) 689, 733
FspBI CTAG 4 cut(s) 584, 725, 855, 912
GlaI GCGC 1 cut(s) 736
GluI GCNGC 2 cut(s) 689, 733
HgaI GACGC 1 cut(s) 1087
HhaI GCGC 1 cut(s) 737
Hin6I GCGC 1 cut(s) 735
HinP1I GCGC 1 cut(s) 735
HindIII AAGCTT 2 cut(s) 109, 1117
HinfI GANTC 8 cut(s) 5, 257, 380, 425, 530, 632, 968, 1106
HphI GGTGA 2 cut(s) 565, 1016
Hpy166II GTNNAC 2 cut(s) 278, 1043
Hpy188I TCNGA 6 cut(s) 34, 81, 86, 207, 262, 1094
Hpy188III TCNNGA 3 cut(s) 473, 514, 777
Hpy8I GTNNAC 2 cut(s) 278, 1043
Hpy99I CGWCG 1 cut(s) 712
HpyAV CCTTC 5 cut(s) 129, 142, 228, 560, 1031
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4IV ACGT 4 cut(s) 499, 760, 892, 973
HpyCH4V TGCA 8 cut(s) 186, 319, 673, 719, 830, 848, 881, 1123
HpyF3I CTNAG 7 cut(s) 198, 206, 387, 523, 942, 1046, 1161
HpySE526I ACGT 4 cut(s) 499, 760, 892, 973
HspAI GCGC 1 cut(s) 735
Kzo9I GATC 3 cut(s) 81, 86, 681
Lsp1109I GCAGC 1 cut(s) 744
LweI GCATC 3 cut(s) 682, 845, 890
MabI ACCWGGT 1 cut(s) 801
MaeI CTAG 4 cut(s) 584, 725, 855, 912
MaeII ACGT 4 cut(s) 499, 760, 892, 973
MaeIII GTNAC 9 cut(s) 239, 302, 503, 518, 571, 647, 809, 857, 888
MalI GATC 3 cut(s) 83, 88, 683
MboI GATC 3 cut(s) 81, 86, 681
MflI RGATCY 1 cut(s) 681
MhlI GDGCHC 2 cut(s) 212, 629
MluCI AATT 9 cut(s) 187, 288, 349, 392, 541, 598, 753, 841, 1143
MlyI GAGTC 2 cut(s) 539, 1100
MnlI CCTC 5 cut(s) 203, 939, 943, 958, 1007
MseI TTAA 4 cut(s) 246, 291, 698, 956
MspR9I CCNGG 2 cut(s) 803, 818
Mva1269I GAATGC 1 cut(s) 832
MvaI CCWGG 2 cut(s) 803, 818
NdeI CATATG 1 cut(s) 430
NdeII GATC 3 cut(s) 81, 86, 681
NlaIV GGNNCC 2 cut(s) 460, 471
NmuCI GTSAC 2 cut(s) 571, 809
PaeR7I CTCGAG 1 cut(s) 95
PcsI WCGNNNNNNNCGW 1 cut(s) 979
PctI GAATGC 1 cut(s) 832
PfeI GAWTC 6 cut(s) 5, 257, 380, 425, 632, 968
PflFI GACNNNGTC 1 cut(s) 803
PflMI CCANNNNNTGG 1 cut(s) 293
PfoI TCCNGGA 1 cut(s) 816
PkrI GCNGC 2 cut(s) 690, 734
PleI GAGTC 2 cut(s) 538, 1100
PpsI GAGTC 2 cut(s) 538, 1100
Ppu21I YACGTR 3 cut(s) 500, 893, 974
PshBI ATTAAT 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 212
Psp6I CCWGG 2 cut(s) 801, 816
PspGI CCWGG 2 cut(s) 801, 816
PspN4I GGNNCC 2 cut(s) 460, 471
PspXI VCTCGAGB 1 cut(s) 95
PstI CTGCAG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 681
PsyI GACNNNGTC 1 cut(s) 803
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
SacI GAGCTC 1 cut(s) 212
SaqAI TTAA 4 cut(s) 246, 291, 698, 956
SatI GCNGC 2 cut(s) 689, 733
Sau3AI GATC 3 cut(s) 81, 86, 681
SchI GAGTC 2 cut(s) 539, 1100
ScrFI CCNGG 2 cut(s) 803, 818
SduI GDGCHC 2 cut(s) 212, 629
SexAI ACCWGGT 1 cut(s) 801
SfaNI GCATC 3 cut(s) 682, 845, 890
SfcI CTRYAG 2 cut(s) 171, 846
Sfr274I CTCGAG 1 cut(s) 95
SlaI CTCGAG 1 cut(s) 95
SmlI CTYRAG 3 cut(s) 95, 514, 1108
SmoI CTYRAG 3 cut(s) 95, 514, 1108
SnaBI TACGTA 1 cut(s) 500
SpeI ACTAGT 1 cut(s) 854
Sse9I AATT 9 cut(s) 187, 288, 349, 392, 541, 598, 753, 841, 1143
SsiI CCGC 1 cut(s) 688
SspMI CTAG 4 cut(s) 584, 725, 855, 912
SstI GAGCTC 1 cut(s) 212
StyD4I CCNGG 2 cut(s) 801, 816
StyI CCWWGG 4 cut(s) 123, 307, 869, 911
TaaI ACNGT 1 cut(s) 509
TaiI ACGT 4 cut(s) 502, 763, 895, 976
TaqI TCGA 4 cut(s) 8, 96, 255, 1104
TaqII GACCGA 1 cut(s) 996
TasI AATT 9 cut(s) 187, 288, 349, 392, 541, 598, 753, 841, 1143
TauI GCSGC 1 cut(s) 691
TfiI GAWTC 6 cut(s) 5, 257, 380, 425, 632, 968
Tru1I TTAA 4 cut(s) 246, 291, 698, 956
Tru9I TTAA 4 cut(s) 246, 291, 698, 956
TscAI CASTG 1 cut(s) 837
TseFI GTSAC 2 cut(s) 571, 809
TseI GCWGC 1 cut(s) 732
Tsp45I GTSAC 2 cut(s) 571, 809
TspDTI ATGAA 3 cut(s) 393, 456, 1002
TspGWI ACGGA 2 cut(s) 31, 625
TspRI CASTG 1 cut(s) 837
Tth111I GACNNNGTC 1 cut(s) 803
Van91I CCANNNNNTGG 1 cut(s) 293
VspI ATTAAT 1 cut(s) 291
XapI RAATTY 3 cut(s) 349, 753, 841
XhoI CTCGAG 1 cut(s) 95
XmaJI CCTAGG 1 cut(s) 911
XspI CTAG 4 cut(s) 584, 725, 855, 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.