Rroxscaffold_3G00250530

Man1-Src1p-C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0011342
Physical Location & Seq
Reverse (-)
44358018 .. 44363115
5098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00250530.1

Sequence Viewer

Length: 1218 bp
ATGTCTTCGACTCCAAAGAAGCGCCCCATACCCAAACCCAGGCCTCACTCTTCTTCTTCGATTCCATTACTCCTCGAACCTCCCCCGAGTCTCTTCCCCACCAAGGACGAGTTTCTCAAGCTCATAGCAGTCCTCGCCATCGCTACCTCCGTCGCTTTCACCGTCAATTTCTTCTACTCCGCTCTCTTCAACCCCTACTCCAAGCCCTTCTGCGATGGCCCCGACGACTCCCTCGACTTCATCTCCGATTATTGTGAGCCGTGTCCGAGTCATGGACGATGCCATGAAGGCCAGTTGGAATGTGATCCAGGATATAAAAAGCATGGGAAGTTATGTGTAGAAGATGGAGTTATTCAGGACACGGCTACGAAACTTGTGGAAAGGGTAGAAATTCGTCTTTGTAAAGCGAATGCTGAATTTTTGTGCTATGGGACTGGAACAATTTGGGTTGAACAGAATGACATATGGAATGACTTAGATAAGAACGAGCTGGTGGAACATGTTGGCTCAGACAATGCAATTTATATGTATGCAAAACAAAGGGCAATGGAGATGATTAATAAAATGTTGGACAGAAGGACAAATTCTCTGGGGGTCAAAGAGTTGAAGTGTCCTGACATGCTAGCTGAACGCTACAAACCATCTTCCTGTCGCATCCGTCAATGGACCTCTGAGCATGCATTACTTATTTTTCCAGTTTGCGCAGCGCTTCTGGGATTGATGTTGTTACTTCGGAAATTCCACCAAAGGCAGTATCTATCGACTAGAGTTGACGAGCTTTACCAACAGATCTGTGAAGAGCTCGAGGAGAAAGCCTTGATGAATAGAGCGAATGATAAATGTGAACCATGGGTTGTTGCCTCTGCATTGCGTGATTCTCTTCTTTCCCTGAAAGAGAGGAAGAACCCTGTATTGTGGAAAAAGGTAGAAGGGTTGATTCAGGAAGATTCCCGTGTGGATCGTTATCCAAAGTTGGTGAAAGGTGAATCAAAAGTAGTATGGGAATGGCAAGTTGAAGGATCTTTGAGCTCTTCGAGGAAAAGTAGAAAGGGTGAGGCTAGCAAATTGAAGTCCAGCAAAGGTACAGAAAGAAGTCCTGATCAACATCTCCATTCTCTCAGGACTGTGACTATTATTCTATCAGGCAAGGTGATGTTTGGTGTCTGTAAGTGGCAAGCCCCTCTAGTCTTTTTCCGTCATTCAGATCCAAATAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MSC

Protein Analysis

405

Amino Acids

46.2

Weight (kDa)

8.53

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MSC PF09402 81 - 336 1.6e-17 Man1-Src1p-C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46560
fragaria_vesca FvH4_5g21210 FvH4_5g21210 FvH4_5g21210 FvH4_5g21210
malus_domestica MD06G1040000.v1.1
prunus_persica Prupe.5G046100_v2.0.a1 Prupe.5G046100_v2.0.a1
pyrus_communis pycom06g03340
rosa_chinensis RchiOBHm_Chr7g0208351
rosa_laevigata RLG00000003220
rosa_multiflora Rmu_sc0006741.1_g000001 Rmu_ssc0000063.1_g000001
rosa_roxburghii Rroxscaffold_3G00250530
rosa_rugosa Rorug07G0103800
rosa_samantha Rh7AG238200 Rh7BG233700 Rh7CG253700 Rh7DG243900
rosa_wichuraiana Rw7G020300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 703
AccBSI CCGCTC 1 cut(s) 182
AciI CCGC 1 cut(s) 180
AclWI GGATC 4 cut(s) 299, 966, 1027, 1199
AcsI RAATTY 4 cut(s) 390, 416, 583, 737
AfaI GTAC 1 cut(s) 1084
AfeI AGCGCT 1 cut(s) 708
AfiI CCNNNNNNNGG 3 cut(s) 39, 103, 272
AflIII ACRYGT 1 cut(s) 499
AgsI TTSAA 5 cut(s) 190, 452, 607, 1016, 1069
AjnI CCWGG 2 cut(s) 38, 307
AluBI AGCT 6 cut(s) 121, 490, 626, 778, 802, 1029
AluI AGCT 6 cut(s) 121, 490, 626, 778, 802, 1029
Alw21I GWGCWC 2 cut(s) 804, 1031
Alw26I GTCTC 1 cut(s) 95
AlwI GGATC 4 cut(s) 299, 966, 1027, 1199
Ama87I CYCGRG 2 cut(s) 85, 803
Aor51HI AGCGCT 1 cut(s) 708
AoxI GGCC 3 cut(s) 41, 217, 289
ApeKI GCWGC 1 cut(s) 704
ApoI RAATTY 4 cut(s) 390, 416, 583, 737
AseI ATTAAT 1 cut(s) 558
AspLEI GCGC 3 cut(s) 24, 704, 709
AspS9I GGNCC 2 cut(s) 218, 666
AsuHPI GGTGA 5 cut(s) 151, 988, 995, 1064, 1162
AsuNHI GCTAGC 2 cut(s) 622, 1058
AvaI CYCGRG 2 cut(s) 85, 803
AvaII GGWCC 1 cut(s) 666
BanII GRGCYC 2 cut(s) 804, 1031
Bbv12I GWGCWC 2 cut(s) 804, 1031
BbvI GCAGC 1 cut(s) 716
BccI CCATC 4 cut(s) 146, 209, 338, 649
BceAI ACGGC 2 cut(s) 244, 378
BciT130I CCWGG 2 cut(s) 40, 309
BclI TGATCA 1 cut(s) 1099
BcoDI GTCTC 1 cut(s) 95
BfaI CTAG 4 cut(s) 623, 765, 1059, 1184
BfoI RGCGCY 2 cut(s) 25, 710
BglI GCCNNNNNGGC 1 cut(s) 288
BglII AGATCT 1 cut(s) 789
BisI GCNGC 1 cut(s) 705
BlsI GCNGC 1 cut(s) 706
Bme1390I CCNGG 2 cut(s) 40, 309
Bme18I GGWCC 1 cut(s) 666
BmeT110I CYCGRG 2 cut(s) 85, 803
BmgT120I GGNCC 2 cut(s) 218, 666
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 2 cut(s) 40, 309
BmsI GCATC 2 cut(s) 269, 663
BmtI GCTAGC 2 cut(s) 626, 1062
BpuEI CTTGAG 1 cut(s) 101
BsaBI GATNNNNATC 2 cut(s) 963, 1104
BsaJI CCNNGG 3 cut(s) 38, 102, 848
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 227, 257
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 103, 272
Bse1I ACTGG 3 cut(s) 292, 439, 695
Bse3DI GCAATG 2 cut(s) 552, 866
Bse8I GATNNNNATC 2 cut(s) 963, 1104
BseBI CCWGG 2 cut(s) 40, 309
BseDI CCNNGG 3 cut(s) 38, 102, 848
BseGI GGATG 1 cut(s) 654
BseJI GATNNNNATC 2 cut(s) 963, 1104
BseLI CCNNNNNNNGG 3 cut(s) 39, 103, 272
BseMI GCAATG 2 cut(s) 552, 866
BseMII CTCAG 3 cut(s) 522, 663, 1132
BseNI ACTGG 3 cut(s) 292, 439, 695
BseRI GAGGAG 2 cut(s) 62, 821
BseXI GCAGC 1 cut(s) 716
BshFI GGCC 3 cut(s) 43, 219, 291
BsiHKAI GWGCWC 2 cut(s) 804, 1031
BsiHKCI CYCGRG 2 cut(s) 85, 803
BslFI GGGAC 1 cut(s) 445
BslI CCNNNNNNNGG 3 cut(s) 39, 103, 272
BsmAI GTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 445
BsmI GAATGC 1 cut(s) 415
BsnI GGCC 3 cut(s) 43, 219, 291
BsoBI CYCGRG 2 cut(s) 85, 803
Bsp1286I GDGCHC 2 cut(s) 804, 1031
Bsp143I GATC 6 cut(s) 304, 789, 958, 1019, 1099, 1204
Bsp19I CCATGG 1 cut(s) 848
BspACI CCGC 1 cut(s) 180
BspANI GGCC 3 cut(s) 43, 219, 291
BspCNI CTCAG 3 cut(s) 521, 664, 1131
BspLI GGNNCC 1 cut(s) 220
BspOI GCTAGC 2 cut(s) 626, 1062
BspPI GGATC 4 cut(s) 299, 966, 1027, 1199
BspQI GCTCTTC 2 cut(s) 792, 1036
BsrBI CCGCTC 1 cut(s) 182
BsrDI GCAATG 2 cut(s) 552, 866
BsrI ACTGG 3 cut(s) 292, 439, 695
BssECI CCNNGG 3 cut(s) 38, 102, 848
BssMI GATC 6 cut(s) 304, 789, 958, 1019, 1099, 1204
BssT1I CCWWGG 2 cut(s) 102, 848
Bst2UI CCWGG 2 cut(s) 40, 309
Bst4CI ACNGT 2 cut(s) 163, 1126
Bst6I CTCTTC 6 cut(s) 55, 98, 191, 792, 885, 1036
BstC8I GCNNGC 4 cut(s) 624, 678, 1060, 1176
BstDEI CTNAG 4 cut(s) 475, 508, 672, 1118
BstDSI CCRYGG 1 cut(s) 848
BstF5I GGATG 1 cut(s) 654
BstH2I RGCGCY 2 cut(s) 25, 710
BstHHI GCGC 3 cut(s) 24, 704, 709
BstKTI GATC 6 cut(s) 307, 792, 961, 1022, 1102, 1207
BstMAI GTCTC 1 cut(s) 95
BstMBI GATC 6 cut(s) 304, 789, 958, 1019, 1099, 1204
BstMWI GCNNNNNNNGC 2 cut(s) 134, 288
BstNI CCWGG 2 cut(s) 40, 309
BstNSI RCATGY 3 cut(s) 503, 622, 680
BstSCI CCNGG 2 cut(s) 38, 307
BstV1I GCAGC 1 cut(s) 716
BstX2I RGATCY 3 cut(s) 789, 1019, 1204
BstYI RGATCY 3 cut(s) 789, 1019, 1204
BsuRI GGCC 3 cut(s) 43, 219, 291
BtgI CCRYGG 1 cut(s) 848
BtgZI GCGATG 2 cut(s) 124, 228
BtsCI GGATG 1 cut(s) 654
Cac8I GCNNGC 4 cut(s) 624, 678, 1060, 1176
CfoI GCGC 3 cut(s) 24, 704, 709
Cfr13I GGNCC 2 cut(s) 218, 666
Csp6I GTAC 1 cut(s) 1083
CviAII CATG 7 cut(s) 272, 284, 323, 500, 619, 677, 849
CviQI GTAC 1 cut(s) 1083
DdeI CTNAG 4 cut(s) 475, 508, 672, 1118
DpnI GATC 6 cut(s) 306, 791, 960, 1021, 1101, 1206
DpnII GATC 6 cut(s) 304, 789, 958, 1019, 1099, 1204
Eam1104I CTCTTC 6 cut(s) 55, 98, 191, 792, 885, 1036
EarI CTCTTC 6 cut(s) 55, 98, 191, 792, 885, 1036
Ecl136II GAGCTC 2 cut(s) 802, 1029
Eco130I CCWWGG 2 cut(s) 102, 848
Eco147I AGGCCT 1 cut(s) 43
Eco24I GRGCYC 2 cut(s) 804, 1031
Eco47I GGWCC 1 cut(s) 666
Eco47III AGCGCT 1 cut(s) 708
Eco53kI GAGCTC 2 cut(s) 802, 1029
Eco88I CYCGRG 2 cut(s) 85, 803
EcoICRI GAGCTC 2 cut(s) 802, 1029
EcoRII CCWGG 2 cut(s) 38, 307
EcoT14I CCWWGG 2 cut(s) 102, 848
EcoT22I ATGCAT 1 cut(s) 682
EcoT38I GRGCYC 2 cut(s) 804, 1031
ErhI CCWWGG 2 cut(s) 102, 848
FaeI CATG 7 cut(s) 275, 287, 326, 503, 622, 680, 852
FaqI GGGAC 1 cut(s) 445
FatI CATG 7 cut(s) 271, 283, 322, 499, 618, 676, 848
FauNDI CATATG 1 cut(s) 464
FbaI TGATCA 1 cut(s) 1099
Fnu4HI GCNGC 1 cut(s) 705
FokI GGATG 1 cut(s) 641
FriOI GRGCYC 2 cut(s) 804, 1031
Fsp4HI GCNGC 1 cut(s) 705
FspBI CTAG 4 cut(s) 623, 765, 1059, 1184
FspI TGCGCA 1 cut(s) 703
GlaI GCGC 3 cut(s) 23, 703, 708
GluI GCNGC 1 cut(s) 705
HaeII RGCGCY 2 cut(s) 25, 710
HaeIII GGCC 3 cut(s) 43, 219, 291
HhaI GCGC 3 cut(s) 24, 704, 709
Hin1II CATG 7 cut(s) 275, 287, 326, 503, 622, 680, 852
Hin6I GCGC 3 cut(s) 22, 702, 707
HinP1I GCGC 3 cut(s) 22, 702, 707
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 9 cut(s) 10, 61, 88, 227, 268, 875, 937, 947, 986
HphI GGTGA 5 cut(s) 151, 988, 995, 1064, 1162
Hpy166II GTNNAC 2 cut(s) 772, 845
Hpy188I TCNGA 6 cut(s) 247, 267, 511, 673, 735, 1204
Hpy188III TCNNGA 5 cut(s) 356, 614, 941, 1097, 1120
Hpy8I GTNNAC 2 cut(s) 772, 845
Hpy99I CGWCG 2 cut(s) 155, 227
HpyAV CCTTC 5 cut(s) 217, 281, 570, 923, 1010
HpyCH4III ACNGT 2 cut(s) 163, 1126
HpyCH4V TGCA 4 cut(s) 518, 533, 680, 866
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 288
HpyF3I CTNAG 4 cut(s) 475, 508, 672, 1118
Hsp92II CATG 7 cut(s) 275, 287, 326, 503, 622, 680, 852
HspAI GCGC 3 cut(s) 22, 702, 707
Ksp22I TGATCA 1 cut(s) 1099
Kzo9I GATC 6 cut(s) 304, 789, 958, 1019, 1099, 1204
LguI GCTCTTC 2 cut(s) 792, 1036
Lsp1109I GCAGC 1 cut(s) 716
LweI GCATC 2 cut(s) 269, 663
MaeI CTAG 4 cut(s) 623, 765, 1059, 1184
MaeIII GTNAC 2 cut(s) 726, 1126
MalI GATC 6 cut(s) 306, 791, 960, 1021, 1101, 1206
MbiI CCGCTC 1 cut(s) 182
MboI GATC 6 cut(s) 304, 789, 958, 1019, 1099, 1204
MflI RGATCY 3 cut(s) 789, 1019, 1204
MhlI GDGCHC 2 cut(s) 804, 1031
MluCI AATT 8 cut(s) 166, 390, 416, 441, 519, 583, 737, 1064
MlyI GAGTC 4 cut(s) 4, 97, 221, 277
MmeI TCCRAC 2 cut(s) 276, 549
Mph1103I ATGCAT 1 cut(s) 682
MseI TTAA 1 cut(s) 558
MspR9I CCNGG 2 cut(s) 40, 309
MteI GCGCNGCGC 1 cut(s) 705
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 2 cut(s) 40, 309
MwoI GCNNNNNNNGC 2 cut(s) 134, 288
NcoI CCATGG 1 cut(s) 848
NdeI CATATG 1 cut(s) 464
NdeII GATC 6 cut(s) 304, 789, 958, 1019, 1099, 1204
NheI GCTAGC 2 cut(s) 622, 1058
NlaIII CATG 7 cut(s) 275, 287, 326, 503, 622, 680, 852
NlaIV GGNNCC 1 cut(s) 220
NmuCI GTSAC 1 cut(s) 1126
NsbI TGCGCA 1 cut(s) 703
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 3 cut(s) 503, 622, 680
PaeI GCATGC 1 cut(s) 680
PaeR7I CTCGAG 1 cut(s) 803
PceI AGGCCT 1 cut(s) 43
PciI ACATGT 1 cut(s) 499
PciSI GCTCTTC 2 cut(s) 792, 1036
PcsI WCGNNNNNNNCGW 3 cut(s) 147, 159, 231
PctI GAATGC 1 cut(s) 415
PfeI GAWTC 5 cut(s) 61, 875, 937, 947, 986
PfoI TCCNGGA 1 cut(s) 307
PkrI GCNGC 1 cut(s) 706
PleI GAGTC 4 cut(s) 4, 96, 221, 276
PpsI GAGTC 4 cut(s) 4, 96, 221, 276
PscI ACATGT 1 cut(s) 499
PshBI ATTAAT 1 cut(s) 558
Psp124BI GAGCTC 2 cut(s) 804, 1031
Psp6I CCWGG 2 cut(s) 38, 307
PspGI CCWGG 2 cut(s) 38, 307
PspN4I GGNNCC 1 cut(s) 220
PspPI GGNCC 2 cut(s) 218, 666
PspXI VCTCGAGB 1 cut(s) 803
PsuI RGATCY 3 cut(s) 789, 1019, 1204
RsaI GTAC 1 cut(s) 1084
RsaNI GTAC 1 cut(s) 1083
SacI GAGCTC 2 cut(s) 804, 1031
SapI GCTCTTC 2 cut(s) 792, 1036
SaqAI TTAA 1 cut(s) 558
SatI GCNGC 1 cut(s) 705
Sau3AI GATC 6 cut(s) 304, 789, 958, 1019, 1099, 1204
Sau96I GGNCC 2 cut(s) 218, 666
SchI GAGTC 4 cut(s) 4, 97, 221, 277
ScrFI CCNGG 2 cut(s) 40, 309
SduI GDGCHC 2 cut(s) 804, 1031
SfaNI GCATC 2 cut(s) 269, 663
Sfr274I CTCGAG 1 cut(s) 803
SinI GGWCC 1 cut(s) 666
SlaI CTCGAG 1 cut(s) 803
SmlI CTYRAG 2 cut(s) 116, 803
SmoI CTYRAG 2 cut(s) 116, 803
SphI GCATGC 1 cut(s) 680
Sse9I AATT 8 cut(s) 166, 390, 416, 441, 519, 583, 737, 1064
SseBI AGGCCT 1 cut(s) 43
SsiI CCGC 1 cut(s) 180
SspMI CTAG 4 cut(s) 623, 765, 1059, 1184
SstI GAGCTC 2 cut(s) 804, 1031
StuI AGGCCT 1 cut(s) 43
StyD4I CCNGG 2 cut(s) 38, 307
StyI CCWWGG 2 cut(s) 102, 848
TaaI ACNGT 2 cut(s) 163, 1126
TaqI TCGA 7 cut(s) 8, 59, 75, 234, 761, 804, 1034
TasI AATT 8 cut(s) 166, 390, 416, 441, 519, 583, 737, 1064
TfiI GAWTC 5 cut(s) 61, 875, 937, 947, 986
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TseFI GTSAC 1 cut(s) 1126
TseI GCWGC 1 cut(s) 704
Tsp45I GTSAC 1 cut(s) 1126
TspDTI ATGAA 3 cut(s) 229, 300, 836
TspGWI ACGGA 3 cut(s) 139, 647, 1184
VpaK11BI GGWCC 1 cut(s) 666
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 4 cut(s) 390, 416, 583, 737
XceI RCATGY 3 cut(s) 503, 622, 680
XhoI CTCGAG 1 cut(s) 803
XspI CTAG 4 cut(s) 623, 765, 1059, 1184
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.