Rh7AG238200

Man1-Src1p-C-terminal domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0011342
Physical Location & Seq
Forward (+)
23275631 .. 23282816
7186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG238200.1

Sequence Viewer

Length: 1233 bp
ATGTCTTCGACTCCAAAGAAGCGCCCTAAACCCAAACCCAGGCCTCACTCTTCTCCTTCTTCGATTCCATTACTCCTCGAACCTCCTCCGAGTCTCTTCCCCACCAAGGACGAGTTTCTCAGGCTCATAGCAGTCCTCGCCATCGCTACCTCCGTCGCTTTCACCTTCAATTTCTTCTACTCCGCTCTCTTCAACCCCTACTCCAAGCCCTTCTGCGATGGCCCCGACGACTCCCTCGACTTCATCTCCGATTATTGTGAGCCGTGTCCGAGTCATGGACGATGCCATGAAGGCCAGTTGGAATGTGATCAAGGATATAAAAAGCATGGGAAGTTATGCGTAGAAGATGGAGTTATTCAGGACACGGCTACGAAACTTGTGGAAAGGGTAGAATTTCGTCTTTGTAAAGCGTATGCTGAATTTTTGTGCTATGGGACTGGAACAATTTGGGTTGAACAGAATGACATATGGAATGACTTAGATAAGAATGAGCTGGCGGAACATGTTGGCTCAGACAATGCAATTTATATGTATGCAAAACAAAGGGCAATGGAGATGATTAATAAAATGTTGGACAGAAGGACAAATTCTCTGGGGGTCAAAGAGTTGAAGTGTCCTGACATGCTAGCAGAACGCTACAAACCATCTTCCTGTCGCATCCGTCAATGGACCTCTGAGCATGCATTACTTATTTTTCCAGTTTGCGCAGCGCTTCTGGGATTGACGTTGTTACTTCGGAAATTCCACCAAAGGCAGTATCTATCGACTAGAGTTGACGAGCTTTACCAAGAGATCTGTGAAGAGCTCGAGGAGAAAGCCTTGATGAATAGAGCGAATGATAAATGTGAACCATGGGTTGTTGCCTCTGCATTGCGTGATTCTCTTCTTTCCCTGAAAGAGAGGAAGAACCCTGTATTGTGGAAAAAGGTAGAAGAGTTGATTCAGGAAGATTCCCGTGTGGATCGTTATCCAAAGTTGGTGAAAGGTGAATCAAAAGTAGTATGGGAATGGCAAGTTGAAGGATCTTTGAGCTCTTCGAGGAAAAGTAGAAAGGGTGAGGCTAGCAAATTGAAGTCCAGCAAAGGTACAGAAAGAAGTCCTGATCAACATCTCCATTCACTCAAGGCTGACCAAAGGCAATTGAATTTTGATACAACGATGTTGAGGCAACCCACTTGGGCTCTCATCTCTCTCACACCCATCAAAGAGCTCTCTGACCCATCTCAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MSC

Protein Analysis

410

Amino Acids

47.02

Weight (kDa)

7.87

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MSC PF09402 82 - 337 2.3e-17 Man1-Src1p-C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011342)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G46560
fragaria_vesca FvH4_5g21210 FvH4_5g21210 FvH4_5g21210 FvH4_5g21210
malus_domestica MD06G1040000.v1.1
prunus_persica Prupe.5G046100_v2.0.a1 Prupe.5G046100_v2.0.a1
pyrus_communis pycom06g03340
rosa_chinensis RchiOBHm_Chr7g0208351
rosa_laevigata RLG00000003220
rosa_multiflora Rmu_sc0006741.1_g000001 Rmu_ssc0000063.1_g000001
rosa_roxburghii Rroxscaffold_3G00250530
rosa_rugosa Rorug07G0103800
rosa_samantha Rh7AG238200 Rh7BG233700 Rh7CG253700 Rh7DG243900
rosa_wichuraiana Rw7G020300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 706
AccBSI CCGCTC 1 cut(s) 185
AciI CCGC 2 cut(s) 183, 497
AclWI GGATC 2 cut(s) 969, 1030
AcsI RAATTY 5 cut(s) 392, 419, 586, 740, 1144
AfaI GTAC 1 cut(s) 1087
AfeI AGCGCT 1 cut(s) 711
AfiI CCNNNNNNNGG 4 cut(s) 39, 106, 275, 1225
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 7 cut(s) 169, 193, 455, 610, 1019, 1072, 1144
AjnI CCWGG 1 cut(s) 38
AluBI AGCT 5 cut(s) 493, 781, 805, 1032, 1210
AluI AGCT 5 cut(s) 493, 781, 805, 1032, 1210
Alw21I GWGCWC 3 cut(s) 807, 1034, 1212
Alw26I GTCTC 1 cut(s) 98
AlwI GGATC 2 cut(s) 969, 1030
Ama87I CYCGRG 1 cut(s) 806
Aor51HI AGCGCT 1 cut(s) 711
AoxI GGCC 3 cut(s) 41, 220, 292
ApeKI GCWGC 1 cut(s) 707
ApoI RAATTY 5 cut(s) 392, 419, 586, 740, 1144
AseI ATTAAT 1 cut(s) 561
AspLEI GCGC 3 cut(s) 24, 707, 712
AspS9I GGNCC 2 cut(s) 221, 669
AsuHPI GGTGA 4 cut(s) 154, 991, 998, 1067
AsuNHI GCTAGC 2 cut(s) 625, 1061
AvaI CYCGRG 1 cut(s) 806
AvaII GGWCC 1 cut(s) 669
BanII GRGCYC 4 cut(s) 807, 1034, 1183, 1212
Bbv12I GWGCWC 3 cut(s) 807, 1034, 1212
BbvI GCAGC 1 cut(s) 719
BccI CCATC 6 cut(s) 149, 212, 341, 652, 1208, 1228
BceAI ACGGC 2 cut(s) 247, 381
BciT130I CCWGG 1 cut(s) 40
BclI TGATCA 2 cut(s) 307, 1102
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 3 cut(s) 626, 768, 1062
BfoI RGCGCY 2 cut(s) 25, 713
BglI GCCNNNNNGGC 1 cut(s) 291
BglII AGATCT 1 cut(s) 792
BisI GCNGC 1 cut(s) 708
BlsI GCNGC 1 cut(s) 709
Bme1390I CCNGG 1 cut(s) 40
Bme18I GGWCC 1 cut(s) 669
BmeT110I CYCGRG 1 cut(s) 806
BmgT120I GGNCC 2 cut(s) 221, 669
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 40
BmsI GCATC 2 cut(s) 272, 666
BmtI GCTAGC 2 cut(s) 629, 1065
BpuEI CTTGAG 1 cut(s) 1106
BsaBI GATNNNNATC 2 cut(s) 966, 1107
BsaJI CCNNGG 3 cut(s) 38, 105, 851
BsaXI ACNNNNNCTCC 4 cut(s) 185, 215, 230, 260
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 106, 275, 1225
Bse1I ACTGG 3 cut(s) 295, 442, 698
Bse3DI GCAATG 2 cut(s) 555, 869
Bse8I GATNNNNATC 2 cut(s) 966, 1107
BseBI CCWGG 1 cut(s) 40
BseDI CCNNGG 3 cut(s) 38, 105, 851
BseGI GGATG 1 cut(s) 657
BseJI GATNNNNATC 2 cut(s) 966, 1107
BseLI CCNNNNNNNGG 4 cut(s) 39, 106, 275, 1225
BseMI GCAATG 2 cut(s) 555, 869
BseMII CTCAG 3 cut(s) 133, 525, 666
BseNI ACTGG 3 cut(s) 295, 442, 698
BseRI GAGGAG 3 cut(s) 65, 75, 824
BseXI GCAGC 1 cut(s) 719
BshFI GGCC 3 cut(s) 43, 222, 294
BsiHKAI GWGCWC 3 cut(s) 807, 1034, 1212
BsiHKCI CYCGRG 1 cut(s) 806
BslFI GGGAC 1 cut(s) 448
BslI CCNNNNNNNGG 4 cut(s) 39, 106, 275, 1225
BsmAI GTCTC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 448
BsnI GGCC 3 cut(s) 43, 222, 294
BsoBI CYCGRG 1 cut(s) 806
Bsp1286I GDGCHC 4 cut(s) 807, 1034, 1183, 1212
Bsp143I GATC 5 cut(s) 307, 792, 961, 1022, 1102
Bsp19I CCATGG 1 cut(s) 851
BspACI CCGC 2 cut(s) 183, 497
BspANI GGCC 3 cut(s) 43, 222, 294
BspCNI CTCAG 3 cut(s) 132, 524, 667
BspLI GGNNCC 1 cut(s) 223
BspOI GCTAGC 2 cut(s) 629, 1065
BspPI GGATC 2 cut(s) 969, 1030
BspQI GCTCTTC 2 cut(s) 795, 1039
BsrBI CCGCTC 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 555, 869
BsrI ACTGG 3 cut(s) 295, 442, 698
BssECI CCNNGG 3 cut(s) 38, 105, 851
BssMI GATC 5 cut(s) 307, 792, 961, 1022, 1102
BssT1I CCWWGG 2 cut(s) 105, 851
Bst2UI CCWGG 1 cut(s) 40
Bst6I CTCTTC 7 cut(s) 55, 101, 194, 795, 888, 927, 1039
BstC8I GCNNGC 4 cut(s) 495, 627, 681, 1063
BstDEI CTNAG 5 cut(s) 119, 478, 511, 675, 1224
BstDSI CCRYGG 1 cut(s) 851
BstF5I GGATG 1 cut(s) 657
BstH2I RGCGCY 2 cut(s) 25, 713
BstHHI GCGC 3 cut(s) 24, 707, 712
BstKTI GATC 5 cut(s) 310, 795, 964, 1025, 1105
BstMAI GTCTC 1 cut(s) 98
BstMBI GATC 5 cut(s) 307, 792, 961, 1022, 1102
BstMWI GCNNNNNNNGC 2 cut(s) 137, 291
BstNI CCWGG 1 cut(s) 40
BstNSI RCATGY 3 cut(s) 506, 625, 683
BstSCI CCNGG 1 cut(s) 38
BstV1I GCAGC 1 cut(s) 719
BstX2I RGATCY 2 cut(s) 792, 1022
BstYI RGATCY 2 cut(s) 792, 1022
BsuRI GGCC 3 cut(s) 43, 222, 294
BtgI CCRYGG 1 cut(s) 851
BtgZI GCGATG 2 cut(s) 127, 231
BtsCI GGATG 1 cut(s) 657
Cac8I GCNNGC 4 cut(s) 495, 627, 681, 1063
CfoI GCGC 3 cut(s) 24, 707, 712
Cfr13I GGNCC 2 cut(s) 221, 669
Csp6I GTAC 1 cut(s) 1086
CviAII CATG 7 cut(s) 275, 287, 326, 503, 622, 680, 852
CviQI GTAC 1 cut(s) 1086
DdeI CTNAG 5 cut(s) 119, 478, 511, 675, 1224
DpnI GATC 5 cut(s) 309, 794, 963, 1024, 1104
DpnII GATC 5 cut(s) 307, 792, 961, 1022, 1102
Eam1104I CTCTTC 7 cut(s) 55, 101, 194, 795, 888, 927, 1039
EarI CTCTTC 7 cut(s) 55, 101, 194, 795, 888, 927, 1039
EciI GGCGGA 1 cut(s) 512
Ecl136II GAGCTC 3 cut(s) 805, 1032, 1210
Eco130I CCWWGG 2 cut(s) 105, 851
Eco147I AGGCCT 1 cut(s) 43
Eco24I GRGCYC 4 cut(s) 807, 1034, 1183, 1212
Eco47I GGWCC 1 cut(s) 669
Eco47III AGCGCT 1 cut(s) 711
Eco53kI GAGCTC 3 cut(s) 805, 1032, 1210
Eco88I CYCGRG 1 cut(s) 806
EcoICRI GAGCTC 3 cut(s) 805, 1032, 1210
EcoRII CCWGG 1 cut(s) 38
EcoT14I CCWWGG 2 cut(s) 105, 851
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 4 cut(s) 807, 1034, 1183, 1212
ErhI CCWWGG 2 cut(s) 105, 851
FaeI CATG 7 cut(s) 278, 290, 329, 506, 625, 683, 855
FaqI GGGAC 1 cut(s) 448
FatI CATG 7 cut(s) 274, 286, 325, 502, 621, 679, 851
FauNDI CATATG 1 cut(s) 467
FbaI TGATCA 2 cut(s) 307, 1102
Fnu4HI GCNGC 1 cut(s) 708
FokI GGATG 1 cut(s) 644
FriOI GRGCYC 4 cut(s) 807, 1034, 1183, 1212
Fsp4HI GCNGC 1 cut(s) 708
FspBI CTAG 3 cut(s) 626, 768, 1062
FspI TGCGCA 1 cut(s) 706
GlaI GCGC 3 cut(s) 23, 706, 711
GluI GCNGC 1 cut(s) 708
HaeII RGCGCY 2 cut(s) 25, 713
HaeIII GGCC 3 cut(s) 43, 222, 294
HhaI GCGC 3 cut(s) 24, 707, 712
Hin1II CATG 7 cut(s) 278, 290, 329, 506, 625, 683, 855
Hin6I GCGC 3 cut(s) 22, 705, 710
HinP1I GCGC 3 cut(s) 22, 705, 710
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 9 cut(s) 10, 64, 91, 230, 271, 878, 940, 950, 989
HphI GGTGA 4 cut(s) 154, 991, 998, 1067
Hpy166II GTNNAC 2 cut(s) 775, 848
Hpy188I TCNGA 7 cut(s) 90, 250, 270, 514, 676, 738, 1216
Hpy188III TCNNGA 4 cut(s) 359, 617, 944, 1100
Hpy8I GTNNAC 2 cut(s) 775, 848
Hpy99I CGWCG 2 cut(s) 158, 230
HpyAV CCTTC 6 cut(s) 66, 175, 220, 284, 573, 1013
HpyCH4IV ACGT 1 cut(s) 725
HpyCH4V TGCA 4 cut(s) 521, 536, 683, 869
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 291
HpyF3I CTNAG 5 cut(s) 119, 478, 511, 675, 1224
HpySE526I ACGT 1 cut(s) 725
Hsp92II CATG 7 cut(s) 278, 290, 329, 506, 625, 683, 855
HspAI GCGC 3 cut(s) 22, 705, 710
Ksp22I TGATCA 2 cut(s) 307, 1102
Kzo9I GATC 5 cut(s) 307, 792, 961, 1022, 1102
LguI GCTCTTC 2 cut(s) 795, 1039
Lsp1109I GCAGC 1 cut(s) 719
LweI GCATC 2 cut(s) 272, 666
MaeI CTAG 3 cut(s) 626, 768, 1062
MaeII ACGT 1 cut(s) 725
MaeIII GTNAC 1 cut(s) 729
MalI GATC 5 cut(s) 309, 794, 963, 1024, 1104
MbiI CCGCTC 1 cut(s) 185
MboI GATC 5 cut(s) 307, 792, 961, 1022, 1102
MfeI CAATTG 1 cut(s) 1139
MflI RGATCY 2 cut(s) 792, 1022
MhlI GDGCHC 4 cut(s) 807, 1034, 1183, 1212
MlyI GAGTC 4 cut(s) 4, 100, 224, 280
MmeI TCCRAC 2 cut(s) 279, 552
Mph1103I ATGCAT 1 cut(s) 685
MseI TTAA 1 cut(s) 561
MspR9I CCNGG 1 cut(s) 40
MteI GCGCNGCGC 1 cut(s) 708
MunI CAATTG 1 cut(s) 1139
MvaI CCWGG 1 cut(s) 40
MwoI GCNNNNNNNGC 2 cut(s) 137, 291
NcoI CCATGG 1 cut(s) 851
NdeI CATATG 1 cut(s) 467
NdeII GATC 5 cut(s) 307, 792, 961, 1022, 1102
NheI GCTAGC 2 cut(s) 625, 1061
NlaIII CATG 7 cut(s) 278, 290, 329, 506, 625, 683, 855
NlaIV GGNNCC 1 cut(s) 223
NsbI TGCGCA 1 cut(s) 706
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 3 cut(s) 506, 625, 683
PaeI GCATGC 1 cut(s) 683
PaeR7I CTCGAG 1 cut(s) 806
PceI AGGCCT 1 cut(s) 43
PciI ACATGT 1 cut(s) 502
PciSI GCTCTTC 2 cut(s) 795, 1039
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 234
PfeI GAWTC 5 cut(s) 64, 878, 940, 950, 989
PkrI GCNGC 1 cut(s) 709
PleI GAGTC 4 cut(s) 4, 99, 224, 279
PpsI GAGTC 4 cut(s) 4, 99, 224, 279
PscI ACATGT 1 cut(s) 502
PshBI ATTAAT 1 cut(s) 561
Psp124BI GAGCTC 3 cut(s) 807, 1034, 1212
Psp6I CCWGG 1 cut(s) 38
PspGI CCWGG 1 cut(s) 38
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 2 cut(s) 221, 669
PspXI VCTCGAGB 1 cut(s) 806
PsuI RGATCY 2 cut(s) 792, 1022
RsaI GTAC 1 cut(s) 1087
RsaNI GTAC 1 cut(s) 1086
SacI GAGCTC 3 cut(s) 807, 1034, 1212
SapI GCTCTTC 2 cut(s) 795, 1039
SaqAI TTAA 1 cut(s) 561
SatI GCNGC 1 cut(s) 708
Sau3AI GATC 5 cut(s) 307, 792, 961, 1022, 1102
Sau96I GGNCC 2 cut(s) 221, 669
SchI GAGTC 4 cut(s) 4, 100, 224, 280
ScrFI CCNGG 1 cut(s) 40
SduI GDGCHC 4 cut(s) 807, 1034, 1183, 1212
SfaNI GCATC 2 cut(s) 272, 666
Sfr274I CTCGAG 1 cut(s) 806
SinI GGWCC 1 cut(s) 669
SlaI CTCGAG 1 cut(s) 806
SmlI CTYRAG 2 cut(s) 806, 1121
SmoI CTYRAG 2 cut(s) 806, 1121
SphI GCATGC 1 cut(s) 683
SseBI AGGCCT 1 cut(s) 43
SsiI CCGC 2 cut(s) 183, 497
SspMI CTAG 3 cut(s) 626, 768, 1062
SstI GAGCTC 3 cut(s) 807, 1034, 1212
StuI AGGCCT 1 cut(s) 43
StyD4I CCNGG 1 cut(s) 38
StyI CCWWGG 2 cut(s) 105, 851
TaiI ACGT 1 cut(s) 728
TaqI TCGA 7 cut(s) 8, 62, 78, 237, 764, 807, 1037
TfiI GAWTC 5 cut(s) 64, 878, 940, 950, 989
Tru1I TTAA 1 cut(s) 561
Tru9I TTAA 1 cut(s) 561
TseI GCWGC 1 cut(s) 707
TspDTI ATGAA 3 cut(s) 232, 303, 839
TspGWI ACGGA 2 cut(s) 142, 650
VpaK11BI GGWCC 1 cut(s) 669
VspI ATTAAT 1 cut(s) 561
XapI RAATTY 5 cut(s) 392, 419, 586, 740, 1144
XceI RCATGY 3 cut(s) 506, 625, 683
XhoI CTCGAG 1 cut(s) 806
XspI CTAG 3 cut(s) 626, 768, 1062
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.