AT5G47860

Protein of unknown function (DUF1350)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013149
Physical Location & Seq
Forward (+)
19381519 .. 19384422
2904 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G47860.1

Sequence Viewer

Length: 1296 bp
ATGGCTACATGTTCCCTCTTACCACCACCGTCTTTTTCTCCGCGCCGGCGATTCTGTAGTTTTTCTCACAATCGCCGGTCTCTACCTTCAATATTCAGTTTCAACAAAGAGCGTGTCTCACGAAGAGTCTTCTGTAGCTCCAAAGAGAACGATATAAACAGAGATCGGAATCAGTCTACAGGAATTCAAGTCTACGGCGAAATCGAGAGGTTATTAACAGAGACTGTAAAAAATTCTCAGAGTAGCTCCGGTGGATCCTCTGATTGGTCAGAAGTTGAGGGAGCATGGGTGCTGAAACCAAGAAACTCGAAACCGAAGATGGTTGTTCATTTCATTGGCGGTATATTTGTCGGTGCAGCACCACAGCTTACTTATCGATTGTTTCTCGAGAGATTAGCGGAGAAGGATGTTTTGGTTATTGCGACACCATATGCTAGTGGTTTTGACCACTTCAATATTGCAGATGAAGTACAATTTAAGTATGATAGATGTTGCCGATCTCTACAAGAAGAAGTGCAGGATCTTCCGTCTTTTGGGATCGGTCATTCACTGGGATCTGTTATTCACCTTCTGATTGGATCAAGGTATGCTGTTCAGCGAAATGGGAACGTATTTATGGCATTCAACAACAAGGAAGCAAGCTTAGCTATTCCTTTGTTCTCCCCAGTTCTTGTCCCGATGGCTCAAAGCCTTGGACCACTGTTGTCACAGGTGGCAACATCCCCAACAATCCGCCTTGGGGCAGAGATGACGCGAAAGCAATTAGAAACGCTTAGCCCTCCCATCATGAAGCAAATTCTGCCTTTAGTGGAACAGCTCCCTCCCTTGTACATGGACTTAGTAAAGGGAAGAGAAGATTTTGTTCCAAAACCAGAAGAAACAAGACGCCTTATAAGATCCTACTATGGAATCTCTAGGAATCTGTTGATAAAATTCGAGGATGATTCAATAGATGAAACATCAATCCTAGCTCAGGTGCTTGGTGTGGAATCATCCATAAGCTCAAAGCTAGACATGTCTATCCGTACACTGCCCGGAGATCACGGTCTCCCTCTGCAACAGGCTCTTCCGGATGTTCCACCAGGAATGGCAGAAGCGGTGAATCGAGGAAGTGAGTTTTTAGCGAATATTGCTGTCGGGACTCCGTGGGAATCTATGGCTAAAGAGGTTGGAGGTTCGCTGGGAATGGACTCGAAGATCCTTCGTGCTGACATGTCCAAAGATCTTGCGCAGCTCGTTGATGCAATCACTTCTTGGATGATTTCAAATATGGGTCCAAAGCTTCTGAGACCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1350

Protein Analysis

431

Amino Acids

47.79

Weight (kDa)

8.2

Isoelectric Point (pI)

59.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1350 PF07082 88 - 356 8.8e-55 Protein of unknown function (DUF1350)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47860
fragaria_vesca FvH4_4g21940
malus_domestica MD13G1135500.v1.1 MD16G1131100.v1.1
prunus_persica Prupe.1G209800_v2.0.a1
pyrus_communis pycom13g11820 pycom16g11560
rosa_chinensis RchiOBHm_Chr4g0428161
rosa_laevigata RLG00000007174
rosa_multiflora Rmu_sc0004940.1_g000008 Rmu_sc0009599.1_g000008
rosa_roxburghii Rroxscaffold_5G00369890
rosa_rugosa Rorug04G0223100
rosa_samantha Rh4AG280400 Rh4BG285900 Rh4CG301200 Rh4DG282900
rosa_wichuraiana Rw4G024350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 893
Acc16I TGCGCA 1 cut(s) 1230
AccI GTMKAC 2 cut(s) 176, 192
AccII CGCG 2 cut(s) 43, 754
AccIII TCCGGA 1 cut(s) 1069
AciI CCGC 5 cut(s) 41, 339, 398, 733, 1097
AclWI GGATC 8 cut(s) 249, 262, 528, 545, 562, 586, 891, 1192
AcsI RAATTY 4 cut(s) 183, 232, 795, 932
AcyI GRCGYC 1 cut(s) 886
AfaI GTAC 3 cut(s) 471, 830, 1027
AfiI CCNNNNNNNGG 4 cut(s) 264, 533, 739, 973
AflIII ACRYGT 3 cut(s) 8, 1014, 1212
AgsI TTSAA 7 cut(s) 90, 103, 188, 454, 625, 948, 1266
AjnI CCWGG 1 cut(s) 1081
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
Alw26I GTCTC 5 cut(s) 84, 121, 215, 1052, 1282
AlwI GGATC 8 cut(s) 249, 262, 528, 545, 562, 586, 891, 1192
AlwNI CAGNNNCTG 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 386
Aor13HI TCCGGA 1 cut(s) 1069
ApeKI GCWGC 2 cut(s) 356, 1231
ApoI RAATTY 4 cut(s) 183, 232, 795, 932
ArsI GACNNNNNNTTYG 2 cut(s) 99, 131
AspLEI GCGC 2 cut(s) 45, 1231
AspS9I GGNCC 2 cut(s) 695, 1274
AsuC2I CCSGG 1 cut(s) 1035
AsuHPI GGTGA 2 cut(s) 557, 1111
AvaI CYCGRG 1 cut(s) 386
AvaII GGWCC 2 cut(s) 695, 1274
BamHI GGATCC 1 cut(s) 254
BbsI GAAGAC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 368, 1243
BccI CCATC 3 cut(s) 313, 673, 791
BceAI ACGGC 1 cut(s) 211
BciT130I CCWGG 1 cut(s) 1083
BcnI CCSGG 1 cut(s) 1035
BcoDI GTCTC 5 cut(s) 84, 121, 215, 1052, 1282
BfaI CTAG 4 cut(s) 435, 915, 968, 1010
BfmI CTRYAG 3 cut(s) 55, 133, 177
BglII AGATCT 1 cut(s) 1222
BisI GCNGC 2 cut(s) 357, 1232
BlpI GCTNAGC 2 cut(s) 643, 773
BlsI GCNGC 2 cut(s) 358, 1233
Bme1390I CCNGG 2 cut(s) 1035, 1083
Bme18I GGWCC 2 cut(s) 695, 1274
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 2 cut(s) 695, 1274
BmiI GGNNCC 2 cut(s) 256, 1275
BmrFI CCNGG 2 cut(s) 1035, 1083
BmrI ACTGGG 2 cut(s) 560, 659
BmsI GCATC 1 cut(s) 1231
BmuI ACTGGG 2 cut(s) 560, 659
BpiI GAAGAC 1 cut(s) 121
BplI GAGNNNNNCTC 2 cut(s) 101, 133
Bpu10I CCTNAGC 1 cut(s) 972
Bpu1102I GCTNAGC 2 cut(s) 643, 773
BpuMI CCSGG 1 cut(s) 1035
Bsa29I ATCGAT 1 cut(s) 376
BsaBI GATNNNNATC 1 cut(s) 168
BsaHI GRCGYC 1 cut(s) 886
BsaI GGTCTC 3 cut(s) 84, 1052, 1282
BsaJI CCNNGG 3 cut(s) 691, 736, 1145
BsaWI WCCGGW 2 cut(s) 248, 1069
BsaXI ACNNNNNCTCC 2 cut(s) 273, 303
Bsc4I CCNNNNNNNGG 4 cut(s) 264, 533, 739, 973
Bse118I RCCGGY 2 cut(s) 45, 75
Bse1I ACTGG 2 cut(s) 555, 665
Bse8I GATNNNNATC 1 cut(s) 168
BseAI TCCGGA 1 cut(s) 1069
BseBI CCWGG 1 cut(s) 1083
BseCI ATCGAT 1 cut(s) 376
BseDI CCNNGG 3 cut(s) 691, 736, 1145
BseGI GGATG 6 cut(s) 412, 719, 946, 992, 1078, 1263
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 4 cut(s) 264, 533, 739, 973
BseMII CTCAG 3 cut(s) 251, 986, 1277
BseNI ACTGG 2 cut(s) 555, 665
BseXI GCAGC 2 cut(s) 368, 1243
BseYI CCCAGC 1 cut(s) 1180
BsgI GTGCAG 2 cut(s) 375, 536
Bsh1236I CGCG 2 cut(s) 43, 754
BshVI ATCGAT 1 cut(s) 376
BsiHKCI CYCGRG 1 cut(s) 386
BsiSI CCGG 5 cut(s) 46, 76, 249, 1035, 1070
BslFI GGGAC 2 cut(s) 659, 1153
BslI CCNNNNNNNGG 4 cut(s) 264, 533, 739, 973
BsmAI GTCTC 5 cut(s) 84, 121, 215, 1052, 1282
BsmFI GGGAC 2 cut(s) 659, 1153
BsmI GAATGC 1 cut(s) 620
Bso31I GGTCTC 3 cut(s) 84, 1052, 1282
BsoBI CYCGRG 1 cut(s) 386
Bsp13I TCCGGA 1 cut(s) 1069
Bsp1407I TGTACA 1 cut(s) 828
Bsp1720I GCTNAGC 2 cut(s) 643, 773
BspACI CCGC 5 cut(s) 41, 339, 398, 733, 1097
BspCNI CTCAG 3 cut(s) 250, 985, 1278
BspDI ATCGAT 1 cut(s) 376
BspEI TCCGGA 1 cut(s) 1069
BspFNI CGCG 2 cut(s) 43, 754
BspHI TCATGA 1 cut(s) 786
BspLI GGNNCC 2 cut(s) 256, 1275
BspPI GGATC 8 cut(s) 249, 262, 528, 545, 562, 586, 891, 1192
BspQI GCTCTTC 1 cut(s) 1071
BspTNI GGTCTC 3 cut(s) 84, 1052, 1282
BsrFI RCCGGY 2 cut(s) 45, 75
BsrGI TGTACA 1 cut(s) 828
BsrI ACTGG 2 cut(s) 555, 665
BssAI RCCGGY 2 cut(s) 45, 75
BssECI CCNNGG 3 cut(s) 691, 736, 1145
BssNI GRCGYC 1 cut(s) 886
BssT1I CCWWGG 2 cut(s) 691, 736
Bst2UI CCWGG 1 cut(s) 1083
Bst4CI ACNGT 4 cut(s) 30, 226, 702, 1046
Bst6I CTCTTC 3 cut(s) 118, 844, 1071
BstACI GRCGYC 1 cut(s) 886
BstAPI GCANNNNNTGC 1 cut(s) 799
BstAUI TGTACA 1 cut(s) 828
BstC8I GCNNGC 2 cut(s) 47, 640
BstDEI CTNAG 6 cut(s) 237, 643, 773, 838, 972, 1286
BstDSI CCRYGG 1 cut(s) 1145
BstENI CCTNNNNNAGG 1 cut(s) 971
BstF5I GGATG 6 cut(s) 412, 719, 946, 992, 1078, 1263
BstFNI CGCG 2 cut(s) 43, 754
BstHHI GCGC 2 cut(s) 45, 1231
BstMAI GTCTC 5 cut(s) 84, 121, 215, 1052, 1282
BstMWI GCNNNNNNNGC 3 cut(s) 644, 799, 1130
BstNI CCWGG 1 cut(s) 1083
BstNSI RCATGY 3 cut(s) 12, 1018, 1216
BstSCI CCNGG 2 cut(s) 1033, 1081
BstSFI CTRYAG 3 cut(s) 55, 133, 177
BstUI CGCG 2 cut(s) 43, 754
BstV1I GCAGC 2 cut(s) 368, 1243
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 6 cut(s) 254, 520, 554, 896, 1197, 1222
BstYI RGATCY 6 cut(s) 254, 520, 554, 896, 1197, 1222
Bsu15I ATCGAT 1 cut(s) 376
BsuTUI ATCGAT 1 cut(s) 376
BtgI CCRYGG 1 cut(s) 1145
BtsCI GGATG 6 cut(s) 412, 719, 946, 992, 1078, 1263
BtsI GCAGTG 1 cut(s) 1028
BtsIMutI CAGTG 3 cut(s) 548, 698, 1028
Cac8I GCNNGC 2 cut(s) 47, 640
CaiI CAGNNNCTG 1 cut(s) 224
CciI TCATGA 1 cut(s) 786
CfoI GCGC 2 cut(s) 45, 1231
Cfr10I RCCGGY 2 cut(s) 45, 75
Cfr13I GGNCC 2 cut(s) 695, 1274
ClaI ATCGAT 1 cut(s) 376
CseI GACGC 2 cut(s) 760, 894
Csp6I GTAC 3 cut(s) 470, 829, 1026
CviAII CATG 6 cut(s) 9, 285, 787, 832, 1015, 1213
CviQI GTAC 3 cut(s) 470, 829, 1026
DdeI CTNAG 6 cut(s) 237, 643, 773, 838, 972, 1286
Eam1104I CTCTTC 3 cut(s) 118, 844, 1071
EarI CTCTTC 3 cut(s) 118, 844, 1071
EciI GGCGGA 1 cut(s) 722
Eco130I CCWWGG 2 cut(s) 691, 736
Eco31I GGTCTC 3 cut(s) 84, 1052, 1282
Eco47I GGWCC 2 cut(s) 695, 1274
Eco88I CYCGRG 1 cut(s) 386
EcoNI CCTNNNNNAGG 1 cut(s) 971
EcoRI GAATTC 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 1081
EcoT14I CCWWGG 2 cut(s) 691, 736
ErhI CCWWGG 2 cut(s) 691, 736
FaeI CATG 6 cut(s) 12, 288, 790, 835, 1018, 1216
FaqI GGGAC 2 cut(s) 659, 1153
FatI CATG 6 cut(s) 8, 284, 786, 831, 1014, 1212
FauNDI CATATG 1 cut(s) 430
FblI GTMKAC 2 cut(s) 176, 192
Fnu4HI GCNGC 2 cut(s) 357, 1232
FokI GGATG 6 cut(s) 419, 706, 953, 979, 1085, 1270
Fsp4HI GCNGC 2 cut(s) 357, 1232
FspBI CTAG 4 cut(s) 435, 915, 968, 1010
FspI TGCGCA 1 cut(s) 1230
GlaI GCGC 2 cut(s) 44, 1230
GluI GCNGC 2 cut(s) 357, 1232
GsaI CCCAGC 1 cut(s) 1184
HapII CCGG 5 cut(s) 46, 76, 249, 1035, 1070
HgaI GACGC 2 cut(s) 760, 894
HhaI GCGC 2 cut(s) 45, 1231
Hin1I GRCGYC 1 cut(s) 886
Hin1II CATG 6 cut(s) 12, 288, 790, 835, 1018, 1216
Hin6I GCGC 2 cut(s) 43, 1229
HinP1I GCGC 2 cut(s) 43, 1229
HindIII AAGCTT 2 cut(s) 640, 1280
HpaII CCGG 5 cut(s) 46, 76, 249, 1035, 1070
HphI GGTGA 2 cut(s) 557, 1111
Hpy166II GTNNAC 3 cut(s) 177, 193, 1028
Hpy188I TCNGA 6 cut(s) 168, 240, 262, 271, 573, 1287
Hpy188III TCNNGA 8 cut(s) 120, 205, 386, 388, 676, 787, 1070, 1138
Hpy8I GTNNAC 3 cut(s) 177, 193, 1028
HpyAV CCTTC 4 cut(s) 96, 397, 578, 1211
HpyCH4III ACNGT 4 cut(s) 30, 226, 702, 1046
HpyCH4IV ACGT 1 cut(s) 609
HpyCH4V TGCA 5 cut(s) 356, 461, 517, 1057, 1244
HpyF10VI GCNNNNNNNGC 3 cut(s) 644, 799, 1130
HpyF3I CTNAG 6 cut(s) 237, 643, 773, 838, 972, 1286
HpySE526I ACGT 1 cut(s) 609
Hsp92I GRCGYC 1 cut(s) 886
Hsp92II CATG 6 cut(s) 12, 288, 790, 835, 1018, 1216
HspAI GCGC 2 cut(s) 43, 1229
Kpn2I TCCGGA 1 cut(s) 1069
KroI GCCGGC 1 cut(s) 45
KroNI GCCGGC 1 cut(s) 47
LguI GCTCTTC 1 cut(s) 1071
LmnI GCTCC 4 cut(s) 143, 251, 281, 822
Lsp1109I GCAGC 2 cut(s) 368, 1243
LweI GCATC 1 cut(s) 1231
MaeI CTAG 4 cut(s) 435, 915, 968, 1010
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 1 cut(s) 705
MflI RGATCY 6 cut(s) 254, 520, 554, 896, 1197, 1222
MluCI AATT 6 cut(s) 183, 232, 473, 761, 795, 932
MlyI GAGTC 3 cut(s) 135, 1135, 1184
MmeI TCCRAC 1 cut(s) 1150
MreI CGCCGGCG 1 cut(s) 45
MroI TCCGGA 1 cut(s) 1069
MroNI GCCGGC 1 cut(s) 45
MseI TTAA 2 cut(s) 215, 477
MspI CCGG 5 cut(s) 46, 76, 249, 1035, 1070
MspR9I CCNGG 2 cut(s) 1035, 1083
Mva1269I GAATGC 1 cut(s) 620
MvaI CCWGG 1 cut(s) 1083
MvnI CGCG 2 cut(s) 43, 754
MwoI GCNNNNNNNGC 3 cut(s) 644, 799, 1130
NaeI GCCGGC 1 cut(s) 47
NciI CCSGG 1 cut(s) 1035
NdeI CATATG 1 cut(s) 430
NgoMIV GCCGGC 1 cut(s) 45
NlaIII CATG 6 cut(s) 12, 288, 790, 835, 1018, 1216
NlaIV GGNNCC 2 cut(s) 256, 1275
NmuCI GTSAC 1 cut(s) 705
NsbI TGCGCA 1 cut(s) 1230
NspI RCATGY 3 cut(s) 12, 1018, 1216
PaeR7I CTCGAG 1 cut(s) 386
PagI TCATGA 1 cut(s) 786
PciI ACATGT 3 cut(s) 8, 1014, 1212
PciSI GCTCTTC 1 cut(s) 1071
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PctI GAATGC 1 cut(s) 620
PdiI GCCGGC 1 cut(s) 47
PfeI GAWTC 8 cut(s) 51, 169, 909, 919, 944, 989, 1102, 1151
PkrI GCNGC 2 cut(s) 358, 1233
PleI GAGTC 3 cut(s) 134, 1135, 1184
PpsI GAGTC 3 cut(s) 134, 1135, 1184
PscI ACATGT 3 cut(s) 8, 1014, 1212
PsiI TTATAA 1 cut(s) 893
Psp6I CCWGG 1 cut(s) 1081
PspFI CCCAGC 1 cut(s) 1180
PspGI CCWGG 1 cut(s) 1081
PspN4I GGNNCC 2 cut(s) 256, 1275
PspPI GGNCC 2 cut(s) 695, 1274
PstNI CAGNNNCTG 1 cut(s) 224
PsuI RGATCY 6 cut(s) 254, 520, 554, 896, 1197, 1222
RsaI GTAC 3 cut(s) 471, 830, 1027
RsaNI GTAC 3 cut(s) 470, 829, 1026
SapI GCTCTTC 1 cut(s) 1071
SaqAI TTAA 2 cut(s) 215, 477
SatI GCNGC 2 cut(s) 357, 1232
Sau96I GGNCC 2 cut(s) 695, 1274
SchI GAGTC 3 cut(s) 135, 1135, 1184
ScrFI CCNGG 2 cut(s) 1035, 1083
SfaNI GCATC 1 cut(s) 1231
SfcI CTRYAG 3 cut(s) 55, 133, 177
Sfr274I CTCGAG 1 cut(s) 386
SgrAI CRCCGGYG 1 cut(s) 45
SinI GGWCC 2 cut(s) 695, 1274
SlaI CTCGAG 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 6 cut(s) 183, 232, 473, 761, 795, 932
SsiI CCGC 5 cut(s) 41, 339, 398, 733, 1097
SspI AATATT 3 cut(s) 93, 457, 1129
SspMI CTAG 4 cut(s) 435, 915, 968, 1010
StyD4I CCNGG 2 cut(s) 1033, 1081
StyI CCWWGG 2 cut(s) 691, 736
TaaI ACNGT 4 cut(s) 30, 226, 702, 1046
TaiI ACGT 1 cut(s) 612
TaqI TCGA 7 cut(s) 204, 308, 376, 387, 936, 1105, 1193
TaqII GACCGA 1 cut(s) 530
TasI AATT 6 cut(s) 183, 232, 473, 761, 795, 932
TatI WGTACW 2 cut(s) 469, 828
TfiI GAWTC 8 cut(s) 51, 169, 909, 919, 944, 989, 1102, 1151
Tru1I TTAA 2 cut(s) 215, 477
Tru9I TTAA 2 cut(s) 215, 477
TscAI CASTG 3 cut(s) 555, 705, 1035
TseFI GTSAC 1 cut(s) 705
TseI GCWGC 2 cut(s) 356, 1231
Tsp45I GTSAC 1 cut(s) 705
TspDTI ATGAA 5 cut(s) 317, 322, 480, 803, 969
TspGWI ACGGA 3 cut(s) 516, 1013, 1134
TspRI CASTG 3 cut(s) 555, 705, 1035
VpaK11BI GGWCC 2 cut(s) 695, 1274
XagI CCTNNNNNAGG 1 cut(s) 971
XapI RAATTY 4 cut(s) 183, 232, 795, 932
XceI RCATGY 3 cut(s) 12, 1018, 1216
XhoI CTCGAG 1 cut(s) 386
XmiI GTMKAC 2 cut(s) 176, 192
XspI CTAG 4 cut(s) 435, 915, 968, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.