Rorug04G0223100

Protein of unknown function (DUF1350)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013149
Physical Location & Seq
Reverse (-)
38307161 .. 38311735
4575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0223100.1

Sequence Viewer

Length: 726 bp
ATGGAGTTCGTCAGCCCTGAAGGTCTTCGTTTAGATGGTCGCCGTCCCATGGAAATGAGGCAAATTCGAGCAGAGATTGGTGTTGTAGCCAAAGCTGACGGTTCTGCTATGTTTGAGATGGGCAACACCAAAGTTGTTGCTGCCGTATATGGCCCTAGAGAGGTCCAAAATAGGAGCCAACAACTGAATGCCAATGCATTGGTGCGATGTGAATACACCATGGCAAATTTTAGTACCGGAGATCGGATGAGAAAACCGAAGGGTGATAGGAGATCCACAGAGATATCGCTAGTTATTCGCCAAACCATGGAAGAATGCATTTTGACAAATTTAATGCCTCGGTCTCAGATAGACATTTTTGTGCAAGTTCTCCAAGCAGATGGAGGAACTAGATCTGCATGTATCAATGCTGCAACCCTGGCCCTTGCAGACGCTGGAATTCCAATGCGGGATCTTGTTACTTCCTGCAGTGCTGGGTACCTTAACAGCACACCTCTACTTGATTTAAACTATATAGAAGATAGTGCTGGAGGTGCTGATGTCACTTTAGGAATTATGCCGAAGTTGGATAAAGTGACTCTTCTTCAGATGGATGCTAAGTTGTCGTTGGATACTTTTGAAAATGTAATGCAACTTGCAATCGAAGGCTGCAAGGCAGTTGCAGATTACATTAGAGAAATATTACTGGAGAATACAAAGCAATTGGAGTATCGTCGAGGGACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1350

Protein Analysis

241

Amino Acids

26.56

Weight (kDa)

5.26

Isoelectric Point (pI)

42.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_PH PF01138 18 - 148 4.5e-41 3' exoribonuclease family, domain 1
RNase_PH_C PF03725 151 - 216 6.7e-13 3' exoribonuclease family, domain 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47860
fragaria_vesca FvH4_4g21940
malus_domestica MD13G1135500.v1.1 MD16G1131100.v1.1
prunus_persica Prupe.1G209800_v2.0.a1
pyrus_communis pycom13g11820 pycom16g11560
rosa_chinensis RchiOBHm_Chr4g0428161
rosa_laevigata RLG00000007174
rosa_multiflora Rmu_sc0004940.1_g000008 Rmu_sc0009599.1_g000008
rosa_roxburghii Rroxscaffold_5G00369890
rosa_rugosa Rorug04G0223100
rosa_samantha Rh4AG280400 Rh4BG285900 Rh4CG301200 Rh4DG282900
rosa_wichuraiana Rw4G024350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 477
AccB1I GGYRCC 1 cut(s) 477
AccB7I CCANNNNNTGG 1 cut(s) 307
AciI CCGC 1 cut(s) 448
AclWI GGATC 2 cut(s) 267, 459
AcsI RAATTY 4 cut(s) 63, 226, 328, 438
AcuI CTGAAG 2 cut(s) 39, 569
AfaI GTAC 2 cut(s) 235, 479
AfiI CCNNNNNNNGG 4 cut(s) 49, 160, 243, 307
AgsI TTSAA 1 cut(s) 620
AjnI CCWGG 1 cut(s) 417
AluBI AGCT 1 cut(s) 95
AluI AGCT 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 348
AlwI GGATC 2 cut(s) 267, 459
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 2 cut(s) 151, 420
ApeKI GCWGC 3 cut(s) 140, 410, 648
ApoI RAATTY 4 cut(s) 63, 226, 328, 438
Asp700I GAANNNNTTC 1 cut(s) 24
Asp718I GGTACC 1 cut(s) 477
AspS9I GGNCC 3 cut(s) 152, 163, 421
AsuHPI GGTGA 1 cut(s) 275
AvaII GGWCC 1 cut(s) 163
BanI GGYRCC 1 cut(s) 477
BbsI GAAGAC 1 cut(s) 17
BbvI GCAGC 3 cut(s) 127, 397, 635
BccI CCATC 4 cut(s) 29, 112, 374, 583
BceAI ACGGC 2 cut(s) 27, 128
BciT130I CCWGG 1 cut(s) 419
BciVI GTATCC 1 cut(s) 604
BcoDI GTCTC 1 cut(s) 348
BfaI CTAG 3 cut(s) 156, 290, 390
BfmI CTRYAG 1 cut(s) 466
BfuI GTATCC 1 cut(s) 604
BglII AGATCT 1 cut(s) 392
BisI GCNGC 3 cut(s) 141, 411, 649
BlsI GCNGC 3 cut(s) 142, 412, 650
Bme1390I CCNGG 1 cut(s) 419
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 3 cut(s) 152, 163, 421
BmiI GGNNCC 2 cut(s) 176, 479
BmrFI CCNGG 1 cut(s) 419
BmsI GCATC 1 cut(s) 583
BpiI GAAGAC 1 cut(s) 17
BpmI CTGGAG 2 cut(s) 549, 707
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 5 cut(s) 48, 219, 306, 338, 417
BsaWI WCCGGW 1 cut(s) 236
Bsc4I CCNNNNNNNGG 4 cut(s) 49, 160, 243, 307
Bse1I ACTGG 1 cut(s) 690
BseBI CCWGG 1 cut(s) 419
BseDI CCNNGG 5 cut(s) 48, 219, 306, 338, 417
BseGI GGATG 2 cut(s) 252, 598
BseLI CCNNNNNNNGG 4 cut(s) 49, 160, 243, 307
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 1 cut(s) 690
BseXI GCAGC 3 cut(s) 127, 397, 635
BseYI CCCAGC 1 cut(s) 473
BshFI GGCC 2 cut(s) 153, 422
BshNI GGYRCC 1 cut(s) 477
BsiSI CCGG 1 cut(s) 237
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 4 cut(s) 49, 160, 243, 307
BsmAI GTCTC 1 cut(s) 348
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 2 cut(s) 193, 320
BsnI GGCC 2 cut(s) 153, 422
Bso31I GGTCTC 1 cut(s) 348
Bsp143I GATC 4 cut(s) 241, 272, 392, 451
Bsp19I CCATGG 3 cut(s) 48, 219, 306
BspACI CCGC 1 cut(s) 448
BspANI GGCC 2 cut(s) 153, 422
BspCNI CTCAG 1 cut(s) 358
BspLI GGNNCC 2 cut(s) 176, 479
BspMAI CTGCAG 1 cut(s) 470
BspPI GGATC 2 cut(s) 267, 459
BspT107I GGYRCC 1 cut(s) 477
BspTNI GGTCTC 1 cut(s) 348
BsrI ACTGG 1 cut(s) 690
BssECI CCNNGG 5 cut(s) 48, 219, 306, 338, 417
BssMI GATC 4 cut(s) 241, 272, 392, 451
BssT1I CCWWGG 3 cut(s) 48, 219, 306
Bst2UI CCWGG 1 cut(s) 419
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 585
BstDEI CTNAG 2 cut(s) 345, 597
BstDSI CCRYGG 3 cut(s) 48, 219, 306
BstF5I GGATG 2 cut(s) 252, 598
BstKTI GATC 4 cut(s) 244, 275, 395, 454
BstMAI GTCTC 1 cut(s) 348
BstMBI GATC 4 cut(s) 241, 272, 392, 451
BstMWI GCNNNNNNNGC 2 cut(s) 419, 533
BstNI CCWGG 1 cut(s) 419
BstNSI RCATGY 1 cut(s) 402
BstSCI CCNGG 1 cut(s) 417
BstSFI CTRYAG 1 cut(s) 466
BstV1I GCAGC 3 cut(s) 127, 397, 635
BstV2I GAAGAC 1 cut(s) 17
BstX2I RGATCY 3 cut(s) 272, 392, 451
BstXI CCANNNNNNTGG 2 cut(s) 199, 380
BstYI RGATCY 3 cut(s) 272, 392, 451
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 2 cut(s) 153, 422
BtgI CCRYGG 3 cut(s) 48, 219, 306
BtgZI GCGATG 1 cut(s) 220
BtsCI GGATG 2 cut(s) 252, 598
BtsI GCAGTG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 475
CaiI CAGNNNCTG 1 cut(s) 434
Cfr13I GGNCC 3 cut(s) 152, 163, 421
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 234, 478
CviAII CATG 4 cut(s) 49, 220, 307, 399
CviJI RGCY 7 cut(s) 15, 89, 95, 153, 177, 422, 648
CviKI_1 RGCY 7 cut(s) 15, 89, 95, 153, 177, 422, 648
CviQI GTAC 2 cut(s) 234, 478
DdeI CTNAG 2 cut(s) 345, 597
DpnI GATC 4 cut(s) 243, 274, 394, 453
DpnII GATC 4 cut(s) 241, 272, 392, 451
DraI TTTAAA 1 cut(s) 507
Eam1104I CTCTTC 1 cut(s) 585
EarI CTCTTC 1 cut(s) 585
Eco130I CCWWGG 3 cut(s) 48, 219, 306
Eco31I GGTCTC 1 cut(s) 348
Eco32I GATATC 1 cut(s) 285
Eco47I GGWCC 1 cut(s) 163
Eco57I CTGAAG 2 cut(s) 39, 569
EcoRI GAATTC 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 417
EcoRV GATATC 1 cut(s) 285
EcoT14I CCWWGG 3 cut(s) 48, 219, 306
EcoT22I ATGCAT 2 cut(s) 199, 320
ErhI CCWWGG 3 cut(s) 48, 219, 306
FaeI CATG 4 cut(s) 52, 223, 310, 402
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FaqI GGGAC 1 cut(s) 30
FatI CATG 4 cut(s) 48, 219, 306, 398
FauI CCCGC 1 cut(s) 441
Fnu4HI GCNGC 3 cut(s) 141, 411, 649
FokI GGATG 2 cut(s) 259, 605
Fsp4HI GCNGC 3 cut(s) 141, 411, 649
FspBI CTAG 3 cut(s) 156, 290, 390
GluI GCNGC 3 cut(s) 141, 411, 649
GsaI CCCAGC 1 cut(s) 477
GsuI CTGGAG 2 cut(s) 549, 707
HaeIII GGCC 2 cut(s) 153, 422
HapII CCGG 1 cut(s) 237
HgaI GACGC 1 cut(s) 440
Hin1II CATG 4 cut(s) 52, 223, 310, 402
HinfI GANTC 1 cut(s) 577
HpaII CCGG 1 cut(s) 237
HphI GGTGA 1 cut(s) 275
Hpy188I TCNGA 3 cut(s) 246, 348, 588
Hpy99I CGWCG 1 cut(s) 717
HpyAV CCTTC 3 cut(s) 14, 253, 638
HpyCH4III ACNGT 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 2 cut(s) 419, 533
HpyF3I CTNAG 2 cut(s) 345, 597
Hsp92II CATG 4 cut(s) 52, 223, 310, 402
KpnI GGTACC 1 cut(s) 481
Kzo9I GATC 4 cut(s) 241, 272, 392, 451
LmnI GCTCC 1 cut(s) 174
LpnPI CCDG 9 cut(s) 30, 250, 404, 420, 431, 459, 478, 513, 671
Lsp1109I GCAGC 3 cut(s) 127, 397, 635
LweI GCATC 1 cut(s) 583
MaeI CTAG 3 cut(s) 156, 290, 390
MaeIII GTNAC 3 cut(s) 457, 541, 574
MalI GATC 4 cut(s) 243, 274, 394, 453
MboI GATC 4 cut(s) 241, 272, 392, 451
MboII GAAGA 5 cut(s) 17, 323, 530, 572, 575
MfeI CAATTG 1 cut(s) 701
MflI RGATCY 3 cut(s) 272, 392, 451
MluCI AATT 6 cut(s) 63, 226, 328, 438, 552, 701
MlyI GAGTC 1 cut(s) 571
MmeI TCCRAC 2 cut(s) 546, 588
MnlI CCTC 7 cut(s) 51, 154, 348, 377, 504, 524, 710
Mph1103I ATGCAT 2 cut(s) 199, 320
MroXI GAANNNNTTC 1 cut(s) 24
MseI TTAA 3 cut(s) 332, 483, 506
MslI CAYNNNNRTG 2 cut(s) 53, 359
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 1 cut(s) 419
MunI CAATTG 1 cut(s) 701
Mva1269I GAATGC 2 cut(s) 193, 320
MvaI CCWGG 1 cut(s) 419
MwoI GCNNNNNNNGC 2 cut(s) 419, 533
NcoI CCATGG 3 cut(s) 48, 219, 306
NdeII GATC 4 cut(s) 241, 272, 392, 451
NlaIII CATG 4 cut(s) 52, 223, 310, 402
NlaIV GGNNCC 2 cut(s) 176, 479
NmuCI GTSAC 2 cut(s) 541, 574
NsiI ATGCAT 2 cut(s) 199, 320
NspI RCATGY 1 cut(s) 402
PctI GAATGC 2 cut(s) 193, 320
PdmI GAANNNNTTC 1 cut(s) 24
PflMI CCANNNNNTGG 1 cut(s) 307
PkrI GCNGC 3 cut(s) 142, 412, 650
PleI GAGTC 1 cut(s) 571
PpsI GAGTC 1 cut(s) 571
Psp6I CCWGG 1 cut(s) 417
PspFI CCCAGC 1 cut(s) 473
PspGI CCWGG 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 176, 479
PspPI GGNCC 3 cut(s) 152, 163, 421
PstI CTGCAG 1 cut(s) 470
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 3 cut(s) 272, 392, 451
RsaI GTAC 2 cut(s) 235, 479
RsaNI GTAC 2 cut(s) 234, 478
RseI CAYNNNNRTG 2 cut(s) 53, 359
SaqAI TTAA 3 cut(s) 332, 483, 506
SatI GCNGC 3 cut(s) 141, 411, 649
Sau3AI GATC 4 cut(s) 241, 272, 392, 451
Sau96I GGNCC 3 cut(s) 152, 163, 421
SchI GAGTC 1 cut(s) 571
ScrFI CCNGG 1 cut(s) 419
SetI ASST 6 cut(s) 25, 97, 165, 483, 496, 535
SfaNI GCATC 1 cut(s) 583
SfcI CTRYAG 1 cut(s) 466
SinI GGWCC 1 cut(s) 163
SmiMI CAYNNNNRTG 2 cut(s) 53, 359
Sse9I AATT 6 cut(s) 63, 226, 328, 438, 552, 701
SsiI CCGC 1 cut(s) 448
SspI AATATT 1 cut(s) 681
SspMI CTAG 3 cut(s) 156, 290, 390
StyD4I CCNGG 1 cut(s) 417
StyI CCWWGG 3 cut(s) 48, 219, 306
TaaI ACNGT 1 cut(s) 101
TaqI TCGA 3 cut(s) 67, 642, 715
TaqII GACCGA 1 cut(s) 330
TasI AATT 6 cut(s) 63, 226, 328, 438, 552, 701
Tru1I TTAA 3 cut(s) 332, 483, 506
Tru9I TTAA 3 cut(s) 332, 483, 506
TscAI CASTG 1 cut(s) 475
TseFI GTSAC 2 cut(s) 541, 574
TseI GCWGC 3 cut(s) 140, 410, 648
Tsp45I GTSAC 2 cut(s) 541, 574
TspRI CASTG 1 cut(s) 475
Van91I CCANNNNNTGG 1 cut(s) 307
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 4 cut(s) 63, 226, 328, 438
XceI RCATGY 1 cut(s) 402
XmnI GAANNNNTTC 1 cut(s) 24
XspI CTAG 3 cut(s) 156, 290, 390
Zsp2I ATGCAT 2 cut(s) 199, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.