Rh4BG285900

Protein of unknown function (DUF1350)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013149
Physical Location & Seq
Forward (+)
45954348 .. 45957707
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG285900.1

Sequence Viewer

Length: 1317 bp
ATGGCTAGCTGGGTTAGGAGTGCACAGCTTCATTCTTATCCTTCTCTTAATCCCCATCAATCTTTGAAGAAGAGGCTTGAAATTCAACAGCAAAGTCATTTTATCAGCCTACCCAATTCTCATCGTCATCTTCGGGGAAGAGTCTTCTGTAGCTTTGATGATGAAGCCACCAAGAAACAATCGCAGCCGCAACCAACTGGGATTCAACTCTACTCTGAGATTGAGAGGTTACTTACTGAGACTGTGAGGCAATCCCAGAGTGGTTGGAATGGTTCGAGTGACTGGAGTCAGATAGAGGGAGCATGGGTTCTGAAACCGAAAACCTTGAAACCCACGGCAGTTGTACATTTTATTGGTGGTATATTTGTTGGAGCTGCCCCTCAGCTCACCTACCGTTTCTTTCTTGAGCGACTAGCAGAGAAGGGAATTTTGGTGATTGCAACACCATATGCTAGTGGGTTTGATCACTTCTTCATTGCAGATGAAGTTCAATTCAAATTTGATCGTTGTTATCGGTTTCTCAAAGACACGATTGAAGATCTTCCTATTTTCGGCGTTGGTCATTCTTTGGGATCTGTCATCCACCTTTTGATTGGTTCGAGATATGCTATTCAAAGAAATGGGAATGTGTTGATGGCATTCAACAACAGGGAGGCGAGCTCCGCTGTTCCTATATTCTCACCTGTTATTGTTCCAGTCGCCCAAAGCCTGGGACCAATTTTAACACAGATCGCATCATCACCAACATTTCGACGAGGGGCTGAAATGACTCTCAGGCAATTTGAAAACCTGAGCCCTCCCATCATGAAGCAAGTTATGCCTTTAGTGGAGCAGCTCCCACCCTTGTATATGGATTTAGTCAATGGAAGAGAAGATTTTGCTCCAAAACCTGAAGAGACTCGGCGACTTATAAAATCATACTACGGTATTTCCAGGAATCTTCTAGTGAGGTTCAAGGATGATACAATTGATGAGACTTCAGTATTAGCACAGGTGCTAAGTTCAGATTCAGCCATAAGCACTATGCTAGACATGTCAACTCGCCTATTGCCCGGAGATCACGCGCTTCCTTTGCAACAGGCTATACCAGATGTGCCTCCAGCAATGGCAGATGCAGTAAATCGCGGGAGTGAGATGTTGGCCAATCTCACTGTGGGAACACCATGGGAGTCTGTTGCTAAAGAAATGGGCAACTCATTAGGCGTAGACTCAACGAAGATCCTTCGTGCAGAAGTGTCCAAAGACCTAGACCTTCTTGTGAATGTTATTGCATCTTGGATGGCTTCCAATACAGGTCCAAGGCTTTTGAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1350

Protein Analysis

438

Amino Acids

48.77

Weight (kDa)

7.81

Isoelectric Point (pI)

48.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1350 PF07082 94 - 362 1.9e-55 Protein of unknown function (DUF1350)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47860
fragaria_vesca FvH4_4g21940
malus_domestica MD13G1135500.v1.1 MD16G1131100.v1.1
prunus_persica Prupe.1G209800_v2.0.a1
pyrus_communis pycom13g11820 pycom16g11560
rosa_chinensis RchiOBHm_Chr4g0428161
rosa_laevigata RLG00000007174
rosa_multiflora Rmu_sc0004940.1_g000008 Rmu_sc0009599.1_g000008
rosa_roxburghii Rroxscaffold_5G00369890
rosa_rugosa Rorug04G0223100
rosa_samantha Rh4AG280400 Rh4BG285900 Rh4CG301200 Rh4DG282900
rosa_wichuraiana Rw4G024350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 911
AccB7I CCANNNNNTGG 1 cut(s) 709
AccI GTMKAC 1 cut(s) 1206
AccII CGCG 2 cut(s) 1064, 1125
AciI CCGC 3 cut(s) 188, 663, 1125
AclWI GGATC 2 cut(s) 580, 1213
AcoI YGGCCR 1 cut(s) 1140
AcsI RAATTY 3 cut(s) 81, 426, 497
AcuI CTGAAG 2 cut(s) 912, 963
AfaI GTAC 1 cut(s) 345
AfiI CCNNNNNNNGG 2 cut(s) 551, 709
AflIII ACRYGT 1 cut(s) 1032
AjnI CCWGG 2 cut(s) 708, 932
AjuI GAANNNNNNNTTGG 2 cut(s) 413, 445
AloI GAACNNNNNNTCC 2 cut(s) 291, 323
AluBI AGCT 7 cut(s) 9, 28, 153, 374, 385, 660, 835
AluI AGCT 7 cut(s) 9, 28, 153, 374, 385, 660, 835
Alw21I GWGCWC 2 cut(s) 25, 662
Alw26I GTCTC 3 cut(s) 233, 890, 968
Alw44I GTGCAC 1 cut(s) 21
AlwI GGATC 2 cut(s) 580, 1213
AoxI GGCC 1 cut(s) 1140
ApaLI GTGCAC 1 cut(s) 21
ApeKI GCWGC 3 cut(s) 184, 374, 832
ApoI RAATTY 3 cut(s) 81, 426, 497
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 1 cut(s) 1066
AspS9I GGNCC 2 cut(s) 713, 1295
AsuC2I CCSGG 1 cut(s) 1053
AsuHPI GGTGA 4 cut(s) 379, 445, 672, 732
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 2 cut(s) 713, 1295
BaeGI GKGCMC 1 cut(s) 25
BalI TGGCCA 1 cut(s) 1142
BanII GRGCYC 2 cut(s) 662, 797
BbsI GAAGAC 1 cut(s) 136
Bbv12I GWGCWC 2 cut(s) 25, 662
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 3 cut(s) 196, 361, 844
BccI CCATC 4 cut(s) 63, 628, 809, 1273
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 2 cut(s) 710, 934
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 1 cut(s) 1053
BcoDI GTCTC 3 cut(s) 233, 890, 968
BfaI CTAG 6 cut(s) 6, 413, 453, 944, 1028, 1247
BfmI CTRYAG 1 cut(s) 148
BglII AGATCT 1 cut(s) 538
BisI GCNGC 4 cut(s) 185, 188, 375, 833
BlsI GCNGC 4 cut(s) 186, 189, 376, 834
Bme1390I CCNGG 3 cut(s) 710, 934, 1053
Bme18I GGWCC 2 cut(s) 713, 1295
BmgT120I GGNCC 2 cut(s) 713, 1295
BmiI GGNNCC 1 cut(s) 714
BmrFI CCNGG 3 cut(s) 710, 934, 1053
BmrI ACTGGG 1 cut(s) 207
BmsI GCATC 3 cut(s) 743, 1102, 1280
BmtI GCTAGC 1 cut(s) 9
BmuI ACTGGG 1 cut(s) 207
BoxI GACNNNNGTC 1 cut(s) 285
BpiI GAAGAC 1 cut(s) 136
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpmI CTGGAG 2 cut(s) 304, 1083
Bpu10I CCTNAGC 2 cut(s) 381, 791
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 1053
BsaJI CCNNGG 4 cut(s) 333, 709, 1163, 1298
BsaXI ACNNNNNCTCC 2 cut(s) 291, 321
Bsc4I CCNNNNNNNGG 2 cut(s) 551, 709
Bse1I ACTGG 3 cut(s) 202, 287, 695
Bse3DI GCAATG 2 cut(s) 474, 1110
BseBI CCWGG 2 cut(s) 710, 934
BseDI CCNNGG 4 cut(s) 333, 709, 1163, 1298
BseGI GGATG 3 cut(s) 579, 964, 1284
BseLI CCNNNNNNNGG 2 cut(s) 551, 709
BseMI GCAATG 2 cut(s) 474, 1110
BseMII CTCAG 5 cut(s) 207, 228, 395, 782, 787
BseNI ACTGG 3 cut(s) 202, 287, 695
BseSI GKGCMC 1 cut(s) 25
BseXI GCAGC 3 cut(s) 196, 361, 844
BseYI CCCAGC 1 cut(s) 9
BsgI GTGCAG 1 cut(s) 1248
Bsh1236I CGCG 2 cut(s) 1064, 1125
BshFI GGCC 1 cut(s) 1142
BsiHKAI GWGCWC 2 cut(s) 25, 662
BsiSI CCGG 1 cut(s) 1053
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 2 cut(s) 551, 709
BsmAI GTCTC 3 cut(s) 233, 890, 968
BsmFI GGGAC 1 cut(s) 726
BsmI GAATGC 1 cut(s) 638
BsnI GGCC 1 cut(s) 1142
Bsp1286I GDGCHC 3 cut(s) 25, 662, 797
Bsp1407I TGTACA 1 cut(s) 343
Bsp143I GATC 7 cut(s) 463, 502, 538, 572, 729, 1057, 1218
Bsp19I CCATGG 1 cut(s) 1163
BspACI CCGC 3 cut(s) 188, 663, 1125
BspANI GGCC 1 cut(s) 1142
BspCNI CTCAG 5 cut(s) 208, 229, 394, 783, 786
BspFNI CGCG 2 cut(s) 1064, 1125
BspHI TCATGA 1 cut(s) 804
BspLI GGNNCC 1 cut(s) 714
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 580, 1213
BsrDI GCAATG 2 cut(s) 474, 1110
BsrGI TGTACA 1 cut(s) 343
BsrI ACTGG 3 cut(s) 202, 287, 695
BssECI CCNNGG 4 cut(s) 333, 709, 1163, 1298
BssMI GATC 7 cut(s) 463, 502, 538, 572, 729, 1057, 1218
BssT1I CCWWGG 2 cut(s) 1163, 1298
Bst2UI CCWGG 2 cut(s) 710, 934
Bst4CI ACNGT 4 cut(s) 244, 395, 926, 1153
Bst6I CTCTTC 4 cut(s) 65, 133, 862, 888
BstAPI GCANNNNNTGC 1 cut(s) 817
BstAUI TGTACA 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 7, 658
BstDEI CTNAG 6 cut(s) 216, 237, 381, 773, 791, 998
BstDSI CCRYGG 2 cut(s) 333, 1163
BstF5I GGATG 3 cut(s) 579, 964, 1284
BstFNI CGCG 2 cut(s) 1064, 1125
BstHHI GCGC 1 cut(s) 1066
BstKTI GATC 7 cut(s) 466, 505, 541, 575, 732, 1060, 1221
BstMAI GTCTC 3 cut(s) 233, 890, 968
BstMBI GATC 7 cut(s) 463, 502, 538, 572, 729, 1057, 1218
BstMWI GCNNNNNNNGC 4 cut(s) 662, 817, 1072, 1309
BstNI CCWGG 2 cut(s) 710, 934
BstNSI RCATGY 1 cut(s) 1036
BstPAI GACNNNNGTC 1 cut(s) 285
BstSCI CCNGG 3 cut(s) 708, 932, 1051
BstSFI CTRYAG 1 cut(s) 148
BstSLI GKGCMC 1 cut(s) 25
BstUI CGCG 2 cut(s) 1064, 1125
BstV1I GCAGC 3 cut(s) 196, 361, 844
BstV2I GAAGAC 1 cut(s) 136
BstX2I RGATCY 3 cut(s) 538, 572, 1218
BstYI RGATCY 3 cut(s) 538, 572, 1218
BsuRI GGCC 1 cut(s) 1142
BtgI CCRYGG 2 cut(s) 333, 1163
BtsCI GGATG 3 cut(s) 579, 964, 1284
BtsIMutI CAGTG 1 cut(s) 1149
Cac8I GCNNGC 2 cut(s) 7, 658
CciI TCATGA 1 cut(s) 804
CfoI GCGC 1 cut(s) 1066
Cfr13I GGNCC 2 cut(s) 713, 1295
Csp6I GTAC 1 cut(s) 344
CspCI CAANNNNNGTGG 2 cut(s) 322, 357
CviAII CATG 5 cut(s) 303, 805, 1033, 1164, 1314
CviQI GTAC 1 cut(s) 344
DdeI CTNAG 6 cut(s) 216, 237, 381, 773, 791, 998
DpnI GATC 7 cut(s) 465, 504, 540, 574, 731, 1059, 1220
DpnII GATC 7 cut(s) 463, 502, 538, 572, 729, 1057, 1218
EaeI YGGCCR 1 cut(s) 1140
Eam1104I CTCTTC 4 cut(s) 65, 133, 862, 888
EarI CTCTTC 4 cut(s) 65, 133, 862, 888
Ecl136II GAGCTC 1 cut(s) 660
Eco130I CCWWGG 2 cut(s) 1163, 1298
Eco24I GRGCYC 2 cut(s) 662, 797
Eco47I GGWCC 2 cut(s) 713, 1295
Eco53kI GAGCTC 1 cut(s) 660
Eco57I CTGAAG 2 cut(s) 912, 963
EcoICRI GAGCTC 1 cut(s) 660
EcoRII CCWGG 2 cut(s) 708, 932
EcoT14I CCWWGG 2 cut(s) 1163, 1298
EcoT38I GRGCYC 2 cut(s) 662, 797
ErhI CCWWGG 2 cut(s) 1163, 1298
FaeI CATG 5 cut(s) 306, 808, 1036, 1167, 1317
FaqI GGGAC 1 cut(s) 726
FatI CATG 5 cut(s) 302, 804, 1032, 1163, 1313
FauI CCCGC 1 cut(s) 1118
FauNDI CATATG 1 cut(s) 448
FbaI TGATCA 1 cut(s) 463
FblI GTMKAC 1 cut(s) 1206
Fnu4HI GCNGC 4 cut(s) 185, 188, 375, 833
FokI GGATG 3 cut(s) 566, 971, 1291
FriOI GRGCYC 2 cut(s) 662, 797
Fsp4HI GCNGC 4 cut(s) 185, 188, 375, 833
FspBI CTAG 6 cut(s) 6, 413, 453, 944, 1028, 1247
GlaI GCGC 1 cut(s) 1065
GluI GCNGC 4 cut(s) 185, 188, 375, 833
GsaI CCCAGC 1 cut(s) 13
GsuI CTGGAG 2 cut(s) 304, 1083
HaeIII GGCC 1 cut(s) 1142
HapII CCGG 1 cut(s) 1053
HhaI GCGC 1 cut(s) 1066
Hin1II CATG 5 cut(s) 306, 808, 1036, 1167, 1317
Hin6I GCGC 1 cut(s) 1064
HinP1I GCGC 1 cut(s) 1064
HincII GTYRAC 1 cut(s) 1038
HindII GTYRAC 1 cut(s) 1038
HinfI GANTC 9 cut(s) 141, 202, 286, 769, 898, 937, 1007, 1169, 1208
HpaII CCGG 1 cut(s) 1053
HphI GGTGA 4 cut(s) 379, 445, 672, 732
Hpy166II GTNNAC 3 cut(s) 23, 1038, 1207
Hpy188I TCNGA 4 cut(s) 217, 291, 312, 1006
Hpy188III TCNNGA 3 cut(s) 404, 600, 805
Hpy8I GTNNAC 3 cut(s) 23, 1038, 1207
Hpy99I CGWCG 1 cut(s) 756
HpyAV CCTTC 4 cut(s) 51, 415, 1232, 1262
HpyCH4III ACNGT 4 cut(s) 244, 395, 926, 1153
HpyCH4V TGCA 7 cut(s) 23, 440, 479, 1075, 1115, 1229, 1271
HpyF10VI GCNNNNNNNGC 4 cut(s) 662, 817, 1072, 1309
HpyF3I CTNAG 6 cut(s) 216, 237, 381, 773, 791, 998
Hsp92II CATG 5 cut(s) 306, 808, 1036, 1167, 1317
HspAI GCGC 1 cut(s) 1064
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 7 cut(s) 463, 502, 538, 572, 729, 1057, 1218
LmnI GCTCC 6 cut(s) 299, 371, 665, 829, 840, 886
Lsp1109I GCAGC 3 cut(s) 196, 361, 844
LweI GCATC 3 cut(s) 743, 1102, 1280
MaeI CTAG 6 cut(s) 6, 413, 453, 944, 1028, 1247
MaeIII GTNAC 2 cut(s) 228, 278
MalI GATC 7 cut(s) 465, 504, 540, 574, 731, 1059, 1220
MboI GATC 7 cut(s) 463, 502, 538, 572, 729, 1057, 1218
MfeI CAATTG 1 cut(s) 966
MflI RGATCY 3 cut(s) 538, 572, 1218
MhlI GDGCHC 3 cut(s) 25, 662, 797
MlsI TGGCCA 1 cut(s) 1142
MluCI AATT 8 cut(s) 81, 115, 426, 491, 497, 717, 779, 966
MluNI TGGCCA 1 cut(s) 1142
MlyI GAGTC 6 cut(s) 150, 295, 763, 892, 1178, 1202
MmeI TCCRAC 2 cut(s) 245, 349
Mox20I TGGCCA 1 cut(s) 1142
MroXI GAANNNNTTC 1 cut(s) 540
MscI TGGCCA 1 cut(s) 1142
MseI TTAA 2 cut(s) 48, 722
Msp20I TGGCCA 1 cut(s) 1142
MspA1I CMGCKG 1 cut(s) 665
MspI CCGG 1 cut(s) 1053
MspR9I CCNGG 3 cut(s) 710, 934, 1053
MunI CAATTG 1 cut(s) 966
Mva1269I GAATGC 1 cut(s) 638
MvaI CCWGG 2 cut(s) 710, 934
MvnI CGCG 2 cut(s) 1064, 1125
MwoI GCNNNNNNNGC 4 cut(s) 662, 817, 1072, 1309
NciI CCSGG 1 cut(s) 1053
NcoI CCATGG 1 cut(s) 1163
NdeI CATATG 1 cut(s) 448
NdeII GATC 7 cut(s) 463, 502, 538, 572, 729, 1057, 1218
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 5 cut(s) 306, 808, 1036, 1167, 1317
NlaIV GGNNCC 1 cut(s) 714
NmeAIII GCCGAG 1 cut(s) 880
NmuCI GTSAC 1 cut(s) 278
NspI RCATGY 1 cut(s) 1036
PagI TCATGA 1 cut(s) 804
PciI ACATGT 1 cut(s) 1032
PctI GAATGC 1 cut(s) 638
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 3 cut(s) 202, 937, 1007
PflMI CCANNNNNTGG 1 cut(s) 709
PfoI TCCNGGA 1 cut(s) 932
PkrI GCNGC 4 cut(s) 186, 189, 376, 834
PleI GAGTC 6 cut(s) 149, 294, 763, 892, 1177, 1202
PpsI GAGTC 6 cut(s) 149, 294, 763, 892, 1177, 1202
PscI ACATGT 1 cut(s) 1032
PshAI GACNNNNGTC 1 cut(s) 285
PsiI TTATAA 1 cut(s) 911
Psp124BI GAGCTC 1 cut(s) 662
Psp6I CCWGG 2 cut(s) 708, 932
PspFI CCCAGC 1 cut(s) 9
PspGI CCWGG 2 cut(s) 708, 932
PspN4I GGNNCC 1 cut(s) 714
PspPI GGNCC 2 cut(s) 713, 1295
PsuI RGATCY 3 cut(s) 538, 572, 1218
RsaI GTAC 1 cut(s) 345
RsaNI GTAC 1 cut(s) 344
SacI GAGCTC 1 cut(s) 662
SaqAI TTAA 2 cut(s) 48, 722
SatI GCNGC 4 cut(s) 185, 188, 375, 833
Sau3AI GATC 7 cut(s) 463, 502, 538, 572, 729, 1057, 1218
Sau96I GGNCC 2 cut(s) 713, 1295
SchI GAGTC 6 cut(s) 150, 295, 763, 892, 1178, 1202
ScrFI CCNGG 3 cut(s) 710, 934, 1053
SduI GDGCHC 3 cut(s) 25, 662, 797
SfaNI GCATC 3 cut(s) 743, 1102, 1280
SfcI CTRYAG 1 cut(s) 148
SinI GGWCC 2 cut(s) 713, 1295
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse9I AATT 8 cut(s) 81, 115, 426, 491, 497, 717, 779, 966
SsiI CCGC 3 cut(s) 188, 663, 1125
SspMI CTAG 6 cut(s) 6, 413, 453, 944, 1028, 1247
SstI GAGCTC 1 cut(s) 662
StyD4I CCNGG 3 cut(s) 708, 932, 1051
StyI CCWWGG 2 cut(s) 1163, 1298
TaaI ACNGT 4 cut(s) 244, 395, 926, 1153
TaqI TCGA 3 cut(s) 275, 599, 751
TasI AATT 8 cut(s) 81, 115, 426, 491, 497, 717, 779, 966
TatI WGTACW 1 cut(s) 343
TauI GCSGC 1 cut(s) 190
TfiI GAWTC 3 cut(s) 202, 937, 1007
Tru1I TTAA 2 cut(s) 48, 722
Tru9I TTAA 2 cut(s) 48, 722
TscAI CASTG 1 cut(s) 1156
TseFI GTSAC 1 cut(s) 278
TseI GCWGC 3 cut(s) 184, 374, 832
Tsp45I GTSAC 1 cut(s) 278
TspDTI ATGAA 5 cut(s) 20, 177, 463, 498, 821
TspRI CASTG 1 cut(s) 1156
Van91I CCANNNNNTGG 1 cut(s) 709
VneI GTGCAC 1 cut(s) 21
VpaK11BI GGWCC 2 cut(s) 713, 1295
XapI RAATTY 3 cut(s) 81, 426, 497
XceI RCATGY 1 cut(s) 1036
XcmI CCANNNNNNNNNTGG 2 cut(s) 590, 1150
XmiI GTMKAC 1 cut(s) 1206
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 6 cut(s) 6, 413, 453, 944, 1028, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.