MD13G1135500.v1.1

Protein of unknown function (DUF1350)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013149
Physical Location & Seq
Reverse (-)
10434678 .. 10438726
4049 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1135500.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGGCCAGTTGCTGGGTGAGGAGTCCGCAGCCCCATCCCAATCTCCACCCTCATCAATCGTTCAGTAAGCGGCTGCTGATTCGGCAGCAGAGCCGCGTCCTCAGCCTCCCGAATTCTCAACGCCGTCGTCGCAGTGGGATTTGCTGCAACTACGACGACGAAACCACCAGGAGACAATCACAACCACCAAGTCCACAGGGTCCACAACCAACTGGGATTCAGCTCTATTCTGAGATTGAGAGGTTACTTACAGAGACTGTGAGGCAATCCCAAGGTCGTTGGGATGGTACAGGAGACTGGAATCAAGTTGAGGGAGCATGGGTTCTCAAACCGAGAAACTCAGAACCCAAGGCCGTTGTACATTTTATCGGTGGTGTTTTTGTTGGAGCTGCCCCTCAGCTTACCTACCGTTTGTTTCTTGAGCGCCTCTCAGAAAAGGGTATTTTGGTGATTGCAACACCATATGCTAGTGGATTTGATCACTTCTTCATTGCAGATGAAACCCAATTCAAATTTGATAGGTGCTATCGGTTTCTTAAAGAGACGGTTCAAGATCTTCCTACTTTCGGCATTGGCCATTCTTTGGGATCTGTCATCCACCTTTTAATCGGTTCAAGATATGCTGTTCAAAGAAATGGGAATGTGCTGATGGCATTCAATAACAAGGAGGCAAGCTCAGCTATTCCTCTGTTCTCACCTGTTCTCGTTCCAGTTGCTCAAGGCCTTGGACCAATTTTATCACAGATTGCATCATCACCGACAGTCCGCCTAGGGGCAGAAATGACTTTGAAGCAATTTGAAAACCTTAGCCCTCCCATCATGAAGCAAGTTCTTCCTTTAGTTGAGCAGCTCCCTCCACTGTATATGGACTTGGTCAAAGGAAGAGAAGATTTTGCTCCAAAACCTGAAGAAACTCAGCGACTTGTAAAATCATACTACGGTATTTCCAGGAATCTACTGGTGAAGTTCAAGGATGATATGATTGATGAGACTTCAACATTGGCAGAGTTGCTAAGTTCAGATTCAGCCATCGGTTCTATGCTAGACATGTCGATTCGCTTGTTACCTGGAGATCACGGACTTCCTTTGCAACAGACGCTCCCGGATGTGCCTCCAGCAATGGCAGACGCAGTAAATCGCGGGAGTGAGATGTTGGCCAATCTCACTGTCGGGACACCATGGGAGACAGTTGCTAAAGAAGTAGGCAGCACATGGGGTGTAGACTCGAAGGTACTTCGTGCAGAAGCATCTAAAGACGTAGGCCTGCTTGTGGATGTGGTTGCGGCATGGATAACTTCAAATACAGGGCCGAAGCTCTTGAAGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1350

Protein Analysis

443

Amino Acids

48.93

Weight (kDa)

8.25

Isoelectric Point (pI)

52.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1350 PF07082 99 - 367 4.5e-55 Protein of unknown function (DUF1350)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47860
fragaria_vesca FvH4_4g21940
malus_domestica MD13G1135500.v1.1 MD16G1131100.v1.1
prunus_persica Prupe.1G209800_v2.0.a1
pyrus_communis pycom13g11820 pycom16g11560
rosa_chinensis RchiOBHm_Chr4g0428161
rosa_laevigata RLG00000007174
rosa_multiflora Rmu_sc0004940.1_g000008 Rmu_sc0009599.1_g000008
rosa_roxburghii Rroxscaffold_5G00369890
rosa_rugosa Rorug04G0223100
rosa_samantha Rh4AG280400 Rh4BG285900 Rh4CG301200 Rh4DG282900
rosa_wichuraiana Rw4G024350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 12, 583
AccI GTMKAC 1 cut(s) 1221
AccII CGCG 2 cut(s) 96, 1140
AciI CCGC 6 cut(s) 26, 70, 94, 766, 1140, 1283
AclWI GGATC 1 cut(s) 595
AcoI YGGCCR 3 cut(s) 3, 574, 1155
AcsI RAATTY 2 cut(s) 112, 512
AcuI CTGAAG 1 cut(s) 927
AfaI GTAC 3 cut(s) 289, 360, 1233
AfiI CCNNNNNNNGG 5 cut(s) 12, 566, 583, 772, 1270
AflIII ACRYGT 1 cut(s) 1047
AjnI CCWGG 3 cut(s) 167, 947, 1066
AloI GAACNNNNNNTCC 2 cut(s) 306, 338
AluBI AGCT 7 cut(s) 223, 389, 400, 675, 680, 850, 1315
AluI AGCT 7 cut(s) 223, 389, 400, 675, 680, 850, 1315
Alw26I GTCTC 6 cut(s) 166, 248, 288, 536, 983, 1178
AlwI GGATC 1 cut(s) 595
AlwNI CAGNNNCTG 2 cut(s) 12, 257
AoxI GGCC 7 cut(s) 3, 351, 574, 721, 1155, 1261, 1307
ApeKI GCWGC 7 cut(s) 28, 73, 85, 144, 389, 847, 1206
ApoI RAATTY 2 cut(s) 112, 512
AspA2I CCTAGG 1 cut(s) 769
AspLEI GCGC 1 cut(s) 426
AspS9I GGNCC 3 cut(s) 200, 728, 1307
AsuC2I CCSGG 1 cut(s) 1103
AsuHPI GGTGA 5 cut(s) 28, 460, 687, 747, 973
AvaII GGWCC 2 cut(s) 200, 728
AvrII CCTAGG 1 cut(s) 769
BalI TGGCCA 3 cut(s) 5, 576, 1157
BbvCI CCTCAGC 2 cut(s) 101, 396
BbvI GCAGC 7 cut(s) 40, 60, 97, 131, 376, 859, 1218
BccI CCATC 5 cut(s) 42, 278, 643, 824, 1037
BceAI ACGGC 3 cut(s) 108, 338, 1309
BciT130I CCWGG 3 cut(s) 169, 949, 1068
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 1 cut(s) 1103
BcoDI GTCTC 6 cut(s) 166, 248, 288, 536, 983, 1178
BfaI CTAG 3 cut(s) 468, 770, 1043
BfoI RGCGCY 1 cut(s) 427
BglII AGATCT 1 cut(s) 553
BlnI CCTAGG 1 cut(s) 769
BlpI GCTNAGC 1 cut(s) 676
Bme1390I CCNGG 4 cut(s) 169, 949, 1068, 1103
Bme18I GGWCC 2 cut(s) 200, 728
BmgT120I GGNCC 3 cut(s) 200, 728, 1307
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 4 cut(s) 169, 949, 1068, 1103
BmrI ACTGGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 758, 1256
BmuI ACTGGG 1 cut(s) 222
BplI GAGNNNNNCTC 4 cut(s) 413, 445, 659, 691
BpmI CTGGAG 2 cut(s) 1089, 1098
Bpu10I CCTNAGC 3 cut(s) 101, 396, 806
Bpu1102I GCTNAGC 1 cut(s) 676
BpuEI CTTGAG 2 cut(s) 440, 702
BpuMI CCSGG 1 cut(s) 1103
BsaJI CCNNGG 5 cut(s) 271, 348, 724, 769, 1178
BsaXI ACNNNNNCTCC 4 cut(s) 306, 336, 1083, 1113
Bsc4I CCNNNNNNNGG 5 cut(s) 12, 566, 583, 772, 1270
Bse1I ACTGG 5 cut(s) 6, 217, 302, 710, 963
Bse3DI GCAATG 2 cut(s) 489, 1125
BseBI CCWGG 3 cut(s) 169, 949, 1068
BseDI CCNNGG 5 cut(s) 271, 348, 724, 769, 1178
BseGI GGATG 6 cut(s) 34, 289, 594, 979, 1111, 1279
BseLI CCNNNNNNNGG 5 cut(s) 12, 566, 583, 772, 1270
BseMI GCAATG 2 cut(s) 489, 1125
BseMII CTCAG 7 cut(s) 115, 222, 354, 410, 444, 690, 929
BseNI ACTGG 5 cut(s) 6, 217, 302, 710, 963
BseRI GAGGAG 1 cut(s) 34
BseXI GCAGC 7 cut(s) 40, 60, 97, 131, 376, 859, 1218
BseYI CCCAGC 1 cut(s) 12
BsgI GTGCAG 1 cut(s) 1260
Bsh1236I CGCG 2 cut(s) 96, 1140
BshFI GGCC 7 cut(s) 5, 353, 576, 723, 1157, 1263, 1309
BsiSI CCGG 1 cut(s) 1103
BslFI GGGAC 1 cut(s) 1186
BslI CCNNNNNNNGG 5 cut(s) 12, 566, 583, 772, 1270
BsmAI GTCTC 6 cut(s) 166, 248, 288, 536, 983, 1178
BsmBI CGTCTC 1 cut(s) 536
BsmFI GGGAC 1 cut(s) 1186
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 7 cut(s) 5, 353, 576, 723, 1157, 1263, 1309
Bsp1407I TGTACA 1 cut(s) 358
Bsp143I GATC 4 cut(s) 478, 553, 587, 1072
Bsp1720I GCTNAGC 1 cut(s) 676
Bsp19I CCATGG 1 cut(s) 1178
BspACI CCGC 6 cut(s) 26, 70, 94, 766, 1140, 1283
BspANI GGCC 7 cut(s) 5, 353, 576, 723, 1157, 1263, 1309
BspCNI CTCAG 7 cut(s) 114, 223, 353, 409, 443, 689, 928
BspFNI CGCG 2 cut(s) 96, 1140
BspHI TCATGA 1 cut(s) 819
BspLI GGNNCC 1 cut(s) 201
BspPI GGATC 1 cut(s) 595
BsrDI GCAATG 2 cut(s) 489, 1125
BsrGI TGTACA 1 cut(s) 358
BsrI ACTGG 5 cut(s) 6, 217, 302, 710, 963
BssECI CCNNGG 5 cut(s) 271, 348, 724, 769, 1178
BssMI GATC 4 cut(s) 478, 553, 587, 1072
BssT1I CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
Bst2UI CCWGG 3 cut(s) 169, 949, 1068
Bst4CI ACNGT 8 cut(s) 259, 410, 547, 763, 861, 941, 1168, 1189
Bst6I CTCTTC 1 cut(s) 877
BstAUI TGTACA 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 673, 1265
BstDEI CTNAG 9 cut(s) 101, 231, 340, 396, 430, 676, 806, 915, 1013
BstDSI CCRYGG 1 cut(s) 1178
BstF5I GGATG 6 cut(s) 34, 289, 594, 979, 1111, 1279
BstFNI CGCG 2 cut(s) 96, 1140
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 1 cut(s) 426
BstKTI GATC 4 cut(s) 481, 556, 590, 1075
BstMAI GTCTC 6 cut(s) 166, 248, 288, 536, 983, 1178
BstMBI GATC 4 cut(s) 478, 553, 587, 1072
BstMWI GCNNNNNNNGC 6 cut(s) 82, 102, 129, 677, 1096, 1321
BstNI CCWGG 3 cut(s) 169, 949, 1068
BstNSI RCATGY 1 cut(s) 1051
BstSCI CCNGG 4 cut(s) 167, 947, 1066, 1101
BstUI CGCG 2 cut(s) 96, 1140
BstV1I GCAGC 7 cut(s) 40, 60, 97, 131, 376, 859, 1218
BstX2I RGATCY 2 cut(s) 553, 587
BstYI RGATCY 2 cut(s) 553, 587
BsuRI GGCC 7 cut(s) 5, 353, 576, 723, 1157, 1263, 1309
BtgI CCRYGG 1 cut(s) 1178
BtsCI GGATG 6 cut(s) 34, 289, 594, 979, 1111, 1279
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 3 cut(s) 139, 857, 1164
Cac8I GCNNGC 2 cut(s) 673, 1265
CaiI CAGNNNCTG 2 cut(s) 12, 257
CciI TCATGA 1 cut(s) 819
CfoI GCGC 1 cut(s) 426
Cfr13I GGNCC 3 cut(s) 200, 728, 1307
CseI GACGC 3 cut(s) 85, 1105, 1136
Csp6I GTAC 3 cut(s) 288, 359, 1232
CviAII CATG 6 cut(s) 318, 820, 1048, 1179, 1212, 1287
CviQI GTAC 3 cut(s) 288, 359, 1232
DdeI CTNAG 9 cut(s) 101, 231, 340, 396, 430, 676, 806, 915, 1013
DpnI GATC 4 cut(s) 480, 555, 589, 1074
DpnII GATC 4 cut(s) 478, 553, 587, 1072
EaeI YGGCCR 3 cut(s) 3, 574, 1155
Eam1104I CTCTTC 1 cut(s) 877
EarI CTCTTC 1 cut(s) 877
EciI GGCGGA 1 cut(s) 755
Eco130I CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
Eco147I AGGCCT 2 cut(s) 723, 1263
Eco47I GGWCC 2 cut(s) 200, 728
Eco57I CTGAAG 1 cut(s) 927
EcoRI GAATTC 1 cut(s) 112
EcoRII CCWGG 3 cut(s) 167, 947, 1066
EcoT14I CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
ErhI CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
Esp3I CGTCTC 1 cut(s) 536
FaeI CATG 6 cut(s) 321, 823, 1051, 1182, 1215, 1290
FaqI GGGAC 1 cut(s) 1186
FatI CATG 6 cut(s) 317, 819, 1047, 1178, 1211, 1286
FauI CCCGC 1 cut(s) 1133
FauNDI CATATG 1 cut(s) 463
FbaI TGATCA 1 cut(s) 478
FblI GTMKAC 1 cut(s) 1221
FokI GGATG 6 cut(s) 21, 296, 581, 986, 1118, 1286
FspBI CTAG 3 cut(s) 468, 770, 1043
GlaI GCGC 1 cut(s) 425
GsaI CCCAGC 1 cut(s) 16
GsuI CTGGAG 2 cut(s) 1089, 1098
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 7 cut(s) 5, 353, 576, 723, 1157, 1263, 1309
HapII CCGG 1 cut(s) 1103
HgaI GACGC 3 cut(s) 85, 1105, 1136
HhaI GCGC 1 cut(s) 426
Hin1II CATG 6 cut(s) 321, 823, 1051, 1182, 1215, 1290
Hin6I GCGC 1 cut(s) 424
HinP1I GCGC 1 cut(s) 424
HinfI GANTC 8 cut(s) 22, 79, 217, 301, 952, 1022, 1054, 1223
HpaII CCGG 1 cut(s) 1103
HphI GGTGA 5 cut(s) 28, 460, 687, 747, 973
Hpy166II GTNNAC 3 cut(s) 194, 203, 1222
Hpy188I TCNGA 4 cut(s) 232, 343, 433, 1021
Hpy188III TCNNGA 7 cut(s) 109, 419, 551, 615, 820, 1171, 1318
Hpy8I GTNNAC 3 cut(s) 194, 203, 1222
Hpy99I CGWCG 4 cut(s) 129, 132, 158, 161
HpyAV CCTTC 1 cut(s) 1222
HpyCH4III ACNGT 8 cut(s) 259, 410, 547, 763, 861, 941, 1168, 1189
HpyCH4IV ACGT 1 cut(s) 1257
HpyCH4V TGCA 6 cut(s) 147, 455, 494, 749, 1090, 1241
HpyF10VI GCNNNNNNNGC 6 cut(s) 82, 102, 129, 677, 1096, 1321
HpyF3I CTNAG 9 cut(s) 101, 231, 340, 396, 430, 676, 806, 915, 1013
HpySE526I ACGT 1 cut(s) 1257
Hsp92II CATG 6 cut(s) 321, 823, 1051, 1182, 1215, 1290
HspAI GCGC 1 cut(s) 424
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 4 cut(s) 478, 553, 587, 1072
LmnI GCTCC 5 cut(s) 314, 386, 855, 901, 1104
Lsp1109I GCAGC 7 cut(s) 40, 60, 97, 131, 376, 859, 1218
LweI GCATC 2 cut(s) 758, 1256
MaeI CTAG 3 cut(s) 468, 770, 1043
MaeII ACGT 1 cut(s) 1257
MaeIII GTNAC 2 cut(s) 243, 1062
MalI GATC 4 cut(s) 480, 555, 589, 1074
MboI GATC 4 cut(s) 478, 553, 587, 1072
MboII GAAGA 6 cut(s) 478, 548, 824, 894, 899, 920
MflI RGATCY 2 cut(s) 553, 587
MlsI TGGCCA 3 cut(s) 5, 576, 1157
MluCI AATT 5 cut(s) 112, 506, 512, 732, 794
MluNI TGGCCA 3 cut(s) 5, 576, 1157
MlyI GAGTC 2 cut(s) 31, 1217
MmeI TCCRAC 1 cut(s) 364
Mox20I TGGCCA 3 cut(s) 5, 576, 1157
MscI TGGCCA 3 cut(s) 5, 576, 1157
MseI TTAA 2 cut(s) 537, 605
Msp20I TGGCCA 3 cut(s) 5, 576, 1157
MspI CCGG 1 cut(s) 1103
MspR9I CCNGG 4 cut(s) 169, 949, 1068, 1103
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 3 cut(s) 169, 949, 1068
MvnI CGCG 2 cut(s) 96, 1140
MwoI GCNNNNNNNGC 6 cut(s) 82, 102, 129, 677, 1096, 1321
NciI CCSGG 1 cut(s) 1103
NcoI CCATGG 1 cut(s) 1178
NdeI CATATG 1 cut(s) 463
NdeII GATC 4 cut(s) 478, 553, 587, 1072
NlaIII CATG 6 cut(s) 321, 823, 1051, 1182, 1215, 1290
NlaIV GGNNCC 1 cut(s) 201
NspI RCATGY 1 cut(s) 1051
PagI TCATGA 1 cut(s) 819
PceI AGGCCT 2 cut(s) 723, 1263
PciI ACATGT 1 cut(s) 1047
PctI GAATGC 1 cut(s) 653
PfeI GAWTC 6 cut(s) 79, 217, 301, 952, 1022, 1054
PflFI GACNNNGTC 1 cut(s) 872
PflMI CCANNNNNTGG 2 cut(s) 12, 583
PfoI TCCNGGA 2 cut(s) 947, 1101
PleI GAGTC 2 cut(s) 30, 1217
PpsI GAGTC 2 cut(s) 30, 1217
PscI ACATGT 1 cut(s) 1047
Psp6I CCWGG 3 cut(s) 167, 947, 1066
PspFI CCCAGC 1 cut(s) 12
PspGI CCWGG 3 cut(s) 167, 947, 1066
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 3 cut(s) 200, 728, 1307
PstNI CAGNNNCTG 2 cut(s) 12, 257
PsuI RGATCY 2 cut(s) 553, 587
PsyI GACNNNGTC 1 cut(s) 872
RsaI GTAC 3 cut(s) 289, 360, 1233
RsaNI GTAC 3 cut(s) 288, 359, 1232
SaqAI TTAA 2 cut(s) 537, 605
Sau3AI GATC 4 cut(s) 478, 553, 587, 1072
Sau96I GGNCC 3 cut(s) 200, 728, 1307
SchI GAGTC 2 cut(s) 31, 1217
ScrFI CCNGG 4 cut(s) 169, 949, 1068, 1103
SfaNI GCATC 2 cut(s) 758, 1256
SinI GGWCC 2 cut(s) 200, 728
SmlI CTYRAG 2 cut(s) 419, 717
SmoI CTYRAG 2 cut(s) 419, 717
Sse9I AATT 5 cut(s) 112, 506, 512, 732, 794
SseBI AGGCCT 2 cut(s) 723, 1263
SsiI CCGC 6 cut(s) 26, 70, 94, 766, 1140, 1283
SspMI CTAG 3 cut(s) 468, 770, 1043
StuI AGGCCT 2 cut(s) 723, 1263
StyD4I CCNGG 4 cut(s) 167, 947, 1066, 1101
StyI CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
TaaI ACNGT 8 cut(s) 259, 410, 547, 763, 861, 941, 1168, 1189
TaiI ACGT 1 cut(s) 1260
TaqI TCGA 2 cut(s) 1052, 1226
TasI AATT 5 cut(s) 112, 506, 512, 732, 794
TatI WGTACW 1 cut(s) 358
TauI GCSGC 3 cut(s) 73, 96, 1286
TfiI GAWTC 6 cut(s) 79, 217, 301, 952, 1022, 1054
Tru1I TTAA 2 cut(s) 537, 605
Tru9I TTAA 2 cut(s) 537, 605
TscAI CASTG 3 cut(s) 139, 864, 1171
TseI GCWGC 7 cut(s) 28, 73, 85, 144, 389, 847, 1206
TspDTI ATGAA 3 cut(s) 478, 513, 836
TspGWI ACGGA 1 cut(s) 1092
TspRI CASTG 3 cut(s) 139, 864, 1171
Tth111I GACNNNGTC 1 cut(s) 872
Van91I CCANNNNNTGG 2 cut(s) 12, 583
VpaK11BI GGWCC 2 cut(s) 200, 728
XapI RAATTY 2 cut(s) 112, 512
XceI RCATGY 1 cut(s) 1051
XcmI CCANNNNNNNNNTGG 1 cut(s) 955
XmaJI CCTAGG 1 cut(s) 769
XmiI GTMKAC 1 cut(s) 1221
XspI CTAG 3 cut(s) 468, 770, 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.