MD16G1131100.v1.1

Protein of unknown function (DUF1350)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013149
Physical Location & Seq
Reverse (-)
10020092 .. 10023967
3876 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1131100.v1.1.491

Sequence Viewer

Length: 1329 bp
ATGGCCAGTTGCTGGGTAAGCAGTCCGCAGCCCCATCCCAATCTCCACCCTCATCAATCTTTCAGTAAGCGGCTGCTGATTCAGTCGCAGAGCCGCGTCCTCAGCCTCTCGAATTCTCAGCGCCGTCGTCGGAGCAGAATCTGCTGCAACTACGACGACGAAACGACTGGGAGACAATCACAACCACCAATTCCACAGGGTCCACAACCAACTGGGATTCAGCTCTACTCCGAGATTGAAAGAGTACTTACAGAGACAGTGAGGCAAGCCCAAGGTCGTTGGGATGGTACAGGCGACTGGACTCAAGTTGAGGGAGCATGGGTTCTCAAACCGAGAAACTCAGAACCCAAGGCCGTTGTACATTTTATTGGTGGCATATTTGTTGGAGCTGCCCCTCAGCTTACCTACCGTTTGTTTCTTGAGCGCCTCTCAGAAAAGGGTATTTTGGTGATTGCAACACCGTATGCTAGTGGATTTGATCACTTCTTCATTGCAGATGAAACCCAAATCAAATTTGATAGGTGTTATCGATTTCTTAAAGAGACGGTTCAAGATCTTCCTACTTTCGGTATTGGCCACTCTTTGGGATCTGTCATCCACCTTTTGATCGGTTCAAGATATGCTGTTCAAAGAAATGGGAATGTGCTGATGGCATTCAATAACAAGGAGGCAAGCTCAACTATTCCTCTGTTCTCACCTGTTGTCGTTCCAGTTGCTCAAGGCCTTGGACCAATTTTAGCACAGATTGCATCATCACCAACAATCCGCCTAGGGGCAGAAATGACTTTGAAGCAATTTGAAAACCTTAGCCCTCCCATCATGAAGCAAGTTCTTCCTTTAGTTGAGCAGCTCCCTCCACTGTATATGGACTTAGTTAATGGAAGAGAAGATTTTGCTCCAAAACCAGAAGAAACTCGGCGACTAGTAAAATCATACTACGGTATTTCCCGGAATCTTCTGGTGAAGTTCAGGGATGATATGATCGACGAGACTTCAACTTTGGCACAGTTGCTAATTTCAAATTCAGCCATCAGTTCTATGCTAGACATGTCGATTCGTTTATTACCTGGAGATCACGGGCTTCCTTTGCAACAGGCTCTCCCAGATGTGCCTCCGGCAATGGCAGATGCAGTAAATCGCGGAAGTGAGATGTTGGCTAATCTCACCGTCGGGACACCATGGGAGACCGTTGCTAAAGAAGTAGGCAGCACATGGGGTGTGGACTCGAAGGTACTTCGTGCAGAAGCATCAAAAGACTTAGGCCTGCTTGTGGATGTGATTGCGTCGTGGATAACTTCAAATGCAGGTCCAAAGCTCTTGAAGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1350

Protein Analysis

443

Amino Acids

48.84

Weight (kDa)

8.27

Isoelectric Point (pI)

49.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1350 PF07082 99 - 367 4.4e-54 Protein of unknown function (DUF1350)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013149)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G47860
fragaria_vesca FvH4_4g21940
malus_domestica MD13G1135500.v1.1 MD16G1131100.v1.1
prunus_persica Prupe.1G209800_v2.0.a1
pyrus_communis pycom13g11820 pycom16g11560
rosa_chinensis RchiOBHm_Chr4g0428161
rosa_laevigata RLG00000007174
rosa_multiflora Rmu_sc0004940.1_g000008 Rmu_sc0009599.1_g000008
rosa_roxburghii Rroxscaffold_5G00369890
rosa_rugosa Rorug04G0223100
rosa_samantha Rh4AG280400 Rh4BG285900 Rh4CG301200 Rh4DG282900
rosa_wichuraiana Rw4G024350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1295
AccB7I CCANNNNNTGG 2 cut(s) 12, 583
AccII CGCG 2 cut(s) 96, 1140
AciI CCGC 5 cut(s) 26, 70, 94, 766, 1140
AclWI GGATC 1 cut(s) 595
AcoI YGGCCR 2 cut(s) 3, 574
AcsI RAATTY 3 cut(s) 112, 512, 1021
AfaI GTAC 4 cut(s) 246, 289, 360, 1233
AfiI CCNNNNNNNGG 5 cut(s) 12, 566, 583, 772, 1270
AflIII ACRYGT 1 cut(s) 1047
AhlI ACTAGT 1 cut(s) 922
AjnI CCWGG 1 cut(s) 1066
AloI GAACNNNNNNTCC 2 cut(s) 306, 338
AluBI AGCT 6 cut(s) 223, 389, 400, 675, 850, 1315
AluI AGCT 6 cut(s) 223, 389, 400, 675, 850, 1315
Alw26I GTCTC 5 cut(s) 166, 248, 536, 983, 1178
AlwI GGATC 1 cut(s) 595
AlwNI CAGNNNCTG 2 cut(s) 12, 141
AoxI GGCC 5 cut(s) 3, 351, 574, 721, 1261
ApeKI GCWGC 6 cut(s) 28, 73, 144, 389, 847, 1206
ApoI RAATTY 3 cut(s) 112, 512, 1021
ArsI GACNNNNNNTTYG 2 cut(s) 982, 1014
AspA2I CCTAGG 1 cut(s) 769
AspLEI GCGC 2 cut(s) 123, 426
AspS9I GGNCC 3 cut(s) 200, 728, 1307
AsuC2I CCSGG 1 cut(s) 949
AsuHPI GGTGA 5 cut(s) 460, 687, 747, 973, 1156
AvaII GGWCC 3 cut(s) 200, 728, 1307
AvrII CCTAGG 1 cut(s) 769
BalI TGGCCA 2 cut(s) 5, 576
BbvCI CCTCAGC 2 cut(s) 101, 396
BbvI GCAGC 6 cut(s) 40, 60, 131, 376, 859, 1218
BccI CCATC 5 cut(s) 42, 278, 643, 824, 1037
BceAI ACGGC 3 cut(s) 108, 338, 1309
BciT130I CCWGG 1 cut(s) 1068
BclI TGATCA 1 cut(s) 478
BcnI CCSGG 1 cut(s) 949
BcoDI GTCTC 5 cut(s) 166, 248, 536, 983, 1178
BcuI ACTAGT 1 cut(s) 922
BfaI CTAG 4 cut(s) 468, 770, 923, 1043
BfoI RGCGCY 2 cut(s) 124, 427
BfuAI ACCTGC 1 cut(s) 1295
BglII AGATCT 1 cut(s) 553
BisI GCNGC 8 cut(s) 29, 71, 74, 94, 145, 390, 848, 1207
BlnI CCTAGG 1 cut(s) 769
BlsI GCNGC 8 cut(s) 30, 72, 75, 95, 146, 391, 849, 1208
BmcAI AGTACT 1 cut(s) 246
Bme1390I CCNGG 2 cut(s) 949, 1068
Bme18I GGWCC 3 cut(s) 200, 728, 1307
BmgT120I GGNCC 3 cut(s) 200, 728, 1307
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 2 cut(s) 949, 1068
BmrI ACTGGG 2 cut(s) 177, 222
BmsI GCATC 3 cut(s) 758, 1117, 1256
BmuI ACTGGG 2 cut(s) 177, 222
BplI GAGNNNNNCTC 4 cut(s) 413, 445, 659, 691
BpmI CTGGAG 1 cut(s) 1089
Bpu10I CCTNAGC 3 cut(s) 101, 396, 806
BpuEI CTTGAG 3 cut(s) 288, 440, 702
BpuMI CCSGG 1 cut(s) 949
Bsa29I ATCGAT 1 cut(s) 529
BsaI GGTCTC 1 cut(s) 1178
BsaJI CCNNGG 5 cut(s) 271, 348, 724, 769, 1178
BsaXI ACNNNNNCTCC 4 cut(s) 306, 336, 1083, 1113
Bsc4I CCNNNNNNNGG 5 cut(s) 12, 566, 583, 772, 1270
Bse1I ACTGG 5 cut(s) 6, 172, 217, 302, 710
Bse3DI GCAATG 2 cut(s) 489, 1125
BseBI CCWGG 1 cut(s) 1068
BseCI ATCGAT 1 cut(s) 529
BseDI CCNNGG 5 cut(s) 271, 348, 724, 769, 1178
BseGI GGATG 5 cut(s) 34, 289, 594, 979, 1279
BseLI CCNNNNNNNGG 5 cut(s) 12, 566, 583, 772, 1270
BseMI GCAATG 2 cut(s) 489, 1125
BseMII CTCAG 5 cut(s) 115, 131, 354, 410, 444
BseNI ACTGG 5 cut(s) 6, 172, 217, 302, 710
BseXI GCAGC 6 cut(s) 40, 60, 131, 376, 859, 1218
BseYI CCCAGC 1 cut(s) 12
BsgI GTGCAG 1 cut(s) 1260
Bsh1236I CGCG 2 cut(s) 96, 1140
BshFI GGCC 5 cut(s) 5, 353, 576, 723, 1263
BshVI ATCGAT 1 cut(s) 529
BsiSI CCGG 2 cut(s) 949, 1115
BslFI GGGAC 1 cut(s) 1186
BslI CCNNNNNNNGG 5 cut(s) 12, 566, 583, 772, 1270
BsmAI GTCTC 5 cut(s) 166, 248, 536, 983, 1178
BsmBI CGTCTC 1 cut(s) 536
BsmFI GGGAC 1 cut(s) 1186
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 5 cut(s) 5, 353, 576, 723, 1263
Bso31I GGTCTC 1 cut(s) 1178
Bsp1407I TGTACA 1 cut(s) 358
Bsp143I GATC 6 cut(s) 478, 553, 587, 606, 981, 1072
Bsp19I CCATGG 1 cut(s) 1178
BspACI CCGC 5 cut(s) 26, 70, 94, 766, 1140
BspANI GGCC 5 cut(s) 5, 353, 576, 723, 1263
BspCNI CTCAG 5 cut(s) 114, 130, 353, 409, 443
BspDI ATCGAT 1 cut(s) 529
BspFNI CGCG 2 cut(s) 96, 1140
BspHI TCATGA 1 cut(s) 819
BspLI GGNNCC 1 cut(s) 201
BspMI ACCTGC 1 cut(s) 1295
BspPI GGATC 1 cut(s) 595
BspTNI GGTCTC 1 cut(s) 1178
BsrDI GCAATG 2 cut(s) 489, 1125
BsrGI TGTACA 1 cut(s) 358
BsrI ACTGG 5 cut(s) 6, 172, 217, 302, 710
BssECI CCNNGG 5 cut(s) 271, 348, 724, 769, 1178
BssMI GATC 6 cut(s) 478, 553, 587, 606, 981, 1072
BssT1I CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
Bst2UI CCWGG 1 cut(s) 1068
Bst4CI ACNGT 9 cut(s) 259, 410, 462, 547, 861, 941, 1008, 1168, 1189
Bst6I CTCTTC 1 cut(s) 877
BstAPI GCANNNNNTGC 2 cut(s) 141, 746
BstAUI TGTACA 1 cut(s) 358
BstC8I GCNNGC 3 cut(s) 267, 673, 1265
BstDEI CTNAG 8 cut(s) 101, 117, 340, 396, 430, 806, 871, 1258
BstDSI CCRYGG 1 cut(s) 1178
BstF5I GGATG 5 cut(s) 34, 289, 594, 979, 1279
BstFNI CGCG 2 cut(s) 96, 1140
BstH2I RGCGCY 2 cut(s) 124, 427
BstHHI GCGC 2 cut(s) 123, 426
BstKTI GATC 6 cut(s) 481, 556, 590, 609, 984, 1075
BstMAI GTCTC 5 cut(s) 166, 248, 536, 983, 1178
BstMBI GATC 6 cut(s) 478, 553, 587, 606, 981, 1072
BstMWI GCNNNNNNNGC 6 cut(s) 18, 102, 141, 746, 1087, 1321
BstNI CCWGG 1 cut(s) 1068
BstNSI RCATGY 1 cut(s) 1051
BstSCI CCNGG 2 cut(s) 947, 1066
BstUI CGCG 2 cut(s) 96, 1140
BstV1I GCAGC 6 cut(s) 40, 60, 131, 376, 859, 1218
BstX2I RGATCY 2 cut(s) 553, 587
BstYI RGATCY 2 cut(s) 553, 587
Bsu15I ATCGAT 1 cut(s) 529
BsuRI GGCC 5 cut(s) 5, 353, 576, 723, 1263
BsuTUI ATCGAT 1 cut(s) 529
BtgI CCRYGG 1 cut(s) 1178
BtsCI GGATG 5 cut(s) 34, 289, 594, 979, 1279
BtsIMutI CAGTG 2 cut(s) 264, 857
BveI ACCTGC 1 cut(s) 1295
Cac8I GCNNGC 3 cut(s) 267, 673, 1265
CaiI CAGNNNCTG 2 cut(s) 12, 141
CciI TCATGA 1 cut(s) 819
CfoI GCGC 2 cut(s) 123, 426
Cfr13I GGNCC 3 cut(s) 200, 728, 1307
ClaI ATCGAT 1 cut(s) 529
CseI GACGC 2 cut(s) 85, 1272
Csp6I GTAC 4 cut(s) 245, 288, 359, 1232
CviAII CATG 5 cut(s) 318, 820, 1048, 1179, 1212
CviQI GTAC 4 cut(s) 245, 288, 359, 1232
DdeI CTNAG 8 cut(s) 101, 117, 340, 396, 430, 806, 871, 1258
DpnI GATC 6 cut(s) 480, 555, 589, 608, 983, 1074
DpnII GATC 6 cut(s) 478, 553, 587, 606, 981, 1072
EaeI YGGCCR 2 cut(s) 3, 574
Eam1104I CTCTTC 1 cut(s) 877
EarI CTCTTC 1 cut(s) 877
EciI GGCGGA 1 cut(s) 755
Eco130I CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
Eco147I AGGCCT 2 cut(s) 723, 1263
Eco31I GGTCTC 1 cut(s) 1178
Eco47I GGWCC 3 cut(s) 200, 728, 1307
EcoRI GAATTC 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 1066
EcoT14I CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
ErhI CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
Esp3I CGTCTC 1 cut(s) 536
FaeI CATG 5 cut(s) 321, 823, 1051, 1182, 1215
FaqI GGGAC 1 cut(s) 1186
FatI CATG 5 cut(s) 317, 819, 1047, 1178, 1211
FbaI TGATCA 1 cut(s) 478
Fnu4HI GCNGC 8 cut(s) 29, 71, 74, 94, 145, 390, 848, 1207
FokI GGATG 5 cut(s) 21, 296, 581, 986, 1286
Fsp4HI GCNGC 8 cut(s) 29, 71, 74, 94, 145, 390, 848, 1207
FspBI CTAG 4 cut(s) 468, 770, 923, 1043
GlaI GCGC 2 cut(s) 122, 425
GluI GCNGC 8 cut(s) 29, 71, 74, 94, 145, 390, 848, 1207
GsaI CCCAGC 1 cut(s) 16
GsuI CTGGAG 1 cut(s) 1089
HaeII RGCGCY 2 cut(s) 124, 427
HaeIII GGCC 5 cut(s) 5, 353, 576, 723, 1263
HapII CCGG 2 cut(s) 949, 1115
HgaI GACGC 2 cut(s) 85, 1272
HhaI GCGC 2 cut(s) 123, 426
Hin1II CATG 5 cut(s) 321, 823, 1051, 1182, 1215
Hin6I GCGC 2 cut(s) 121, 424
HinP1I GCGC 2 cut(s) 121, 424
HinfI GANTC 7 cut(s) 79, 138, 217, 301, 952, 1054, 1223
HpaII CCGG 2 cut(s) 949, 1115
HphI GGTGA 5 cut(s) 460, 687, 747, 973, 1156
Hpy166II GTNNAC 2 cut(s) 203, 1222
Hpy188I TCNGA 4 cut(s) 132, 232, 343, 433
Hpy188III TCNNGA 7 cut(s) 109, 419, 551, 615, 820, 1171, 1318
Hpy8I GTNNAC 2 cut(s) 203, 1222
Hpy99I CGWCG 7 cut(s) 129, 132, 158, 161, 989, 1172, 1288
HpyAV CCTTC 1 cut(s) 1222
HpyCH4III ACNGT 9 cut(s) 259, 410, 462, 547, 861, 941, 1008, 1168, 1189
HpyCH4V TGCA 8 cut(s) 147, 455, 494, 749, 1090, 1130, 1241, 1304
HpyF10VI GCNNNNNNNGC 6 cut(s) 18, 102, 141, 746, 1087, 1321
HpyF3I CTNAG 8 cut(s) 101, 117, 340, 396, 430, 806, 871, 1258
Hsp92II CATG 5 cut(s) 321, 823, 1051, 1182, 1215
HspAI GCGC 2 cut(s) 121, 424
Ksp22I TGATCA 1 cut(s) 478
Kzo9I GATC 6 cut(s) 478, 553, 587, 606, 981, 1072
LmnI GCTCC 5 cut(s) 132, 314, 386, 855, 901
Lsp1109I GCAGC 6 cut(s) 40, 60, 131, 376, 859, 1218
LweI GCATC 3 cut(s) 758, 1117, 1256
MaeI CTAG 4 cut(s) 468, 770, 923, 1043
MalI GATC 6 cut(s) 480, 555, 589, 608, 983, 1074
MboI GATC 6 cut(s) 478, 553, 587, 606, 981, 1072
MboII GAAGA 7 cut(s) 478, 548, 824, 894, 899, 920, 947
MflI RGATCY 2 cut(s) 553, 587
MlsI TGGCCA 2 cut(s) 5, 576
MluCI AATT 7 cut(s) 112, 189, 512, 732, 794, 1014, 1021
MluNI TGGCCA 2 cut(s) 5, 576
MlyI GAGTC 2 cut(s) 295, 1217
MmeI TCCRAC 2 cut(s) 110, 364
Mox20I TGGCCA 2 cut(s) 5, 576
MscI TGGCCA 2 cut(s) 5, 576
MseI TTAA 2 cut(s) 537, 876
Msp20I TGGCCA 2 cut(s) 5, 576
MspI CCGG 2 cut(s) 949, 1115
MspR9I CCNGG 2 cut(s) 949, 1068
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 1 cut(s) 1068
MvnI CGCG 2 cut(s) 96, 1140
MwoI GCNNNNNNNGC 6 cut(s) 18, 102, 141, 746, 1087, 1321
NciI CCSGG 1 cut(s) 949
NcoI CCATGG 1 cut(s) 1178
NdeII GATC 6 cut(s) 478, 553, 587, 606, 981, 1072
NlaIII CATG 5 cut(s) 321, 823, 1051, 1182, 1215
NlaIV GGNNCC 1 cut(s) 201
NmeAIII GCCGAG 1 cut(s) 895
NspI RCATGY 1 cut(s) 1051
PagI TCATGA 1 cut(s) 819
PceI AGGCCT 2 cut(s) 723, 1263
PciI ACATGT 1 cut(s) 1047
PctI GAATGC 1 cut(s) 653
PfeI GAWTC 5 cut(s) 79, 138, 217, 952, 1054
PflMI CCANNNNNTGG 2 cut(s) 12, 583
PfoI TCCNGGA 1 cut(s) 947
PkrI GCNGC 8 cut(s) 30, 72, 75, 95, 146, 391, 849, 1208
PleI GAGTC 2 cut(s) 295, 1217
PpsI GAGTC 2 cut(s) 295, 1217
PscI ACATGT 1 cut(s) 1047
Psp6I CCWGG 1 cut(s) 1066
PspFI CCCAGC 1 cut(s) 12
PspGI CCWGG 1 cut(s) 1066
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 3 cut(s) 200, 728, 1307
PstNI CAGNNNCTG 2 cut(s) 12, 141
PsuI RGATCY 2 cut(s) 553, 587
RsaI GTAC 4 cut(s) 246, 289, 360, 1233
RsaNI GTAC 4 cut(s) 245, 288, 359, 1232
SaqAI TTAA 2 cut(s) 537, 876
SatI GCNGC 8 cut(s) 29, 71, 74, 94, 145, 390, 848, 1207
Sau3AI GATC 6 cut(s) 478, 553, 587, 606, 981, 1072
Sau96I GGNCC 3 cut(s) 200, 728, 1307
ScaI AGTACT 1 cut(s) 246
SchI GAGTC 2 cut(s) 295, 1217
ScrFI CCNGG 2 cut(s) 949, 1068
SfaNI GCATC 3 cut(s) 758, 1117, 1256
SinI GGWCC 3 cut(s) 200, 728, 1307
SmlI CTYRAG 3 cut(s) 303, 419, 717
SmoI CTYRAG 3 cut(s) 303, 419, 717
SpeI ACTAGT 1 cut(s) 922
Sse9I AATT 7 cut(s) 112, 189, 512, 732, 794, 1014, 1021
SseBI AGGCCT 2 cut(s) 723, 1263
SsiI CCGC 5 cut(s) 26, 70, 94, 766, 1140
SspMI CTAG 4 cut(s) 468, 770, 923, 1043
StuI AGGCCT 2 cut(s) 723, 1263
StyD4I CCNGG 2 cut(s) 947, 1066
StyI CCWWGG 5 cut(s) 271, 348, 724, 769, 1178
TaaI ACNGT 9 cut(s) 259, 410, 462, 547, 861, 941, 1008, 1168, 1189
TaqI TCGA 5 cut(s) 110, 529, 984, 1052, 1226
TasI AATT 7 cut(s) 112, 189, 512, 732, 794, 1014, 1021
TatI WGTACW 2 cut(s) 244, 358
TauI GCSGC 2 cut(s) 73, 96
TfiI GAWTC 5 cut(s) 79, 138, 217, 952, 1054
Tru1I TTAA 2 cut(s) 537, 876
Tru9I TTAA 2 cut(s) 537, 876
TscAI CASTG 2 cut(s) 264, 864
TseI GCWGC 6 cut(s) 28, 73, 144, 389, 847, 1206
TspDTI ATGAA 3 cut(s) 478, 513, 836
TspRI CASTG 2 cut(s) 264, 864
Van91I CCANNNNNTGG 2 cut(s) 12, 583
VpaK11BI GGWCC 3 cut(s) 200, 728, 1307
XapI RAATTY 3 cut(s) 112, 512, 1021
XceI RCATGY 1 cut(s) 1051
XmaJI CCTAGG 1 cut(s) 769
XspI CTAG 4 cut(s) 468, 770, 923, 1043
ZrmI AGTACT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.