AT5G50020

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010663
Physical Location & Seq
Forward (+)
20351157 .. 20354436
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50020.1

Sequence Viewer

Length: 1224 bp
ATGGCTGGACGGGTCTTCGAAGCCTGGAAGGGAAGTAATAAGTTTCTGTTTGGTGGGAGATTGATATTTGGGCCAGATGCTTGGTCTATTCCTTTTACCTTCCTGCTCATCATAACTCCCGTTTGTTTCTTTTCTGTATTTGTTGCAACACATCTTCGCCGCGAGCTCTTACCAAACAATGCGGGACATGTGTTCTTAGTGGCAGGAGTTCTATTTACTGTCTTTGTACTGATCCTTCTCTTCCTCACTTCTGCGCGGGATCCTGGAATCGTTCCAAGAAATTCGCATCCGCCAGAGGAAGAACTATGCTATGATACAACTGTATCAAGTGATGGAAGACAAACACCCACTGTTCAAATTCCTAGGACGAAGGAAGTCATGGTTTATGGCGTTTCTGTTAGAGTTAAATATTGTGATACATGCATGCTTTATCGGCCTCCTCGATGCTCCCATTGTTCCATTTGCAACAACTGTGTTGAGCGCTTTGATCATCATTGCCCTTGGAGAAACTATAGGTACTTCTTTATGTTTGTTTCTTCGGCAACTATTCTCTGCATCTACATTTTTTCGATGTCGGCATTATACATCAAGGTTCTGATGGATAACCATCAAGGTACTGTGTGGAGGGCAATGCGAGAATCACCCTGGGCGGTTATGCTGATGATATATTGCTTTATTTCCCTGTGGTTTGTTGGAGGGCTTACTGGGTTTCACTTGTACCTCATTAGCACAAACCAGACGACCTATGAGAATTTCCGGTACAGATCAGACAATAGAATCAACGTTTATAACCGTGGGTGTTCAAACAATTTTTTTGAGACATTTTGCTCAAAAGTTAAACCCTCAAGGAATGACTTCAGAGCGTTCATCAAAGAAGAACCTCCAAGGAATATTACATTGGCTACCACATGGGAACGACCAGAAGAAGCAGATGAAGAAAACAGGGAGGAACGGCGCCAGAAGGTGGAAGACGATCTAGACATCGATGAAGATGTTATGAAACTGCAGCAGCGTTTAAATGACGAAGAGGGCAGCGATACAGCGCATCACAAGATCGATATTGACCAAATGAGAATCGGGTCTAATGAGAGAGCGCCAACAATTCGATCAGAAGCCAGGCATGGAAACTGGGGAGCAAGAAGCAATGCACAGGAGGAGGACGTAATTGCTGGTTCTTCAGTAAGAGAAAGTAGAAGCTATGCAGCTGCAGAGGAGGGACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04391 map04391
Pfam Domains
Protein Families
DHHC

Protein Analysis

407

Amino Acids

47.1

Weight (kDa)

6.43

Isoelectric Point (pI)

39.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 136 - 253 1e-29 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24630 AT5G50020 AT5G50020 AT5G50020
fragaria_vesca FvH4_2g13200
malus_domestica MD10G1032800.v1.1
prunus_persica Prupe.8G038400_v2.0.a1 Prupe.8G038400_v2.0.a1 Prupe.8G038400_v2.0.a1
pyrus_communis pycom10g02090
rosa_chinensis RchiOBHm_Chr6g0274491
rosa_laevigata RLG00000013537
rosa_multiflora Rmu_sc0001289.1_g000020
rosa_roxburghii Rroxscaffold_7G00194180
rosa_rugosa Rorug06G0082200
rosa_samantha Rh6AG198500 Rh6BG201800 Rh6CG202600 Rh6DG193100
rosa_wichuraiana Rw6G017260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 789
AccB1I GGYRCC 1 cut(s) 954
AccB7I CCANNNNNTGG 1 cut(s) 964
AccII CGCG 2 cut(s) 162, 256
AciI CCGC 5 cut(s) 160, 182, 256, 290, 650
AclI AACGTT 1 cut(s) 783
AclWI GGATC 3 cut(s) 226, 254, 267
AcsI RAATTY 3 cut(s) 280, 357, 751
AcuI CTGAAG 2 cut(s) 841, 1161
AcyI GRCGYC 1 cut(s) 955
AfaI GTAC 5 cut(s) 228, 518, 616, 719, 761
AfeI AGCGCT 1 cut(s) 482
AfiI CCNNNNNNNGG 1 cut(s) 964
AflIII ACRYGT 1 cut(s) 187
AgsI TTSAA 2 cut(s) 356, 804
AjnI CCWGG 4 cut(s) 23, 262, 644, 1115
AjuI GAANNNNNNNTTGG 2 cut(s) 881, 913
AluBI AGCT 3 cut(s) 166, 1197, 1205
AluI AGCT 3 cut(s) 166, 1197, 1205
Alw21I GWGCWC 1 cut(s) 168
Alw26I GTCTC 1 cut(s) 812
AlwI GGATC 3 cut(s) 226, 254, 267
Aor51HI AGCGCT 1 cut(s) 482
AoxI GGCC 2 cut(s) 71, 434
ApeKI GCWGC 5 cut(s) 1006, 1009, 1032, 1202, 1205
ApoI RAATTY 3 cut(s) 280, 357, 751
Asp700I GAANNNNTTC 1 cut(s) 854
AspA2I CCTAGG 1 cut(s) 362
AspLEI GCGC 5 cut(s) 256, 483, 957, 1045, 1096
AspS9I GGNCC 1 cut(s) 71
AsuHPI GGTGA 1 cut(s) 633
AsuII TTCGAA 1 cut(s) 18
AvrII CCTAGG 1 cut(s) 362
BaeI ACNNNNGTAYC 4 cut(s) 306, 306, 339, 339
BamHI GGATCC 1 cut(s) 259
BanI GGYRCC 1 cut(s) 954
BanII GRGCYC 1 cut(s) 168
BarI GAAGNNNNNNTAC 2 cut(s) 219, 251
BbsI GAAGAC 3 cut(s) 7, 343, 975
Bbv12I GWGCWC 1 cut(s) 168
BbvI GCAGC 5 cut(s) 1018, 1021, 1044, 1192, 1214
BccI CCATC 3 cut(s) 326, 592, 615
BceAI ACGGC 1 cut(s) 968
BciT130I CCWGG 4 cut(s) 25, 264, 646, 1117
BclI TGATCA 1 cut(s) 487
BcoDI GTCTC 1 cut(s) 812
BfaI CTAG 2 cut(s) 363, 977
BfmI CTRYAG 3 cut(s) 511, 1004, 1206
BfoI RGCGCY 3 cut(s) 484, 958, 1097
BisI GCNGC 6 cut(s) 160, 1007, 1010, 1033, 1203, 1206
BlnI CCTAGG 1 cut(s) 362
BlsI GCNGC 6 cut(s) 161, 1008, 1011, 1034, 1204, 1207
Bme1390I CCNGG 4 cut(s) 25, 264, 646, 1117
BmgT120I GGNCC 1 cut(s) 71
BmiI GGNNCC 2 cut(s) 261, 956
BmrFI CCNGG 4 cut(s) 25, 264, 646, 1117
BmrI ACTGGG 2 cut(s) 714, 1138
BmsI GCATC 5 cut(s) 67, 295, 434, 564, 1054
BmuI ACTGGG 2 cut(s) 714, 1138
BpiI GAAGAC 3 cut(s) 7, 343, 975
Bpu14I TTCGAA 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 829
Bsa29I ATCGAT 2 cut(s) 984, 1056
BsaBI GATNNNNATC 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 955
BsaJI CCNNGG 6 cut(s) 362, 500, 644, 645, 793, 884
BsaWI WCCGGW 1 cut(s) 756
BsaXI ACNNNNNCTCC 1 cut(s) 1205
Bsc4I CCNNNNNNNGG 1 cut(s) 964
Bse1I ACTGG 2 cut(s) 709, 1133
Bse3DI GCAATG 3 cut(s) 493, 636, 1150
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 4 cut(s) 25, 264, 646, 1117
BseCI ATCGAT 2 cut(s) 984, 1056
BseDI CCNNGG 6 cut(s) 362, 500, 644, 645, 793, 884
BseGI GGATG 1 cut(s) 286
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 1 cut(s) 964
BseMI GCAATG 3 cut(s) 493, 636, 1150
BseNI ACTGG 2 cut(s) 709, 1133
BseRI GAGGAG 2 cut(s) 429, 1169
BseXI GCAGC 5 cut(s) 1018, 1021, 1044, 1192, 1214
Bsh1236I CGCG 2 cut(s) 162, 256
BshFI GGCC 2 cut(s) 73, 436
BshNI GGYRCC 1 cut(s) 954
BshVI ATCGAT 2 cut(s) 984, 1056
BsiHKAI GWGCWC 1 cut(s) 168
BsiSI CCGG 1 cut(s) 757
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 964
BsmAI GTCTC 1 cut(s) 812
BsmFI GGGAC 1 cut(s) 198
BsnI GGCC 2 cut(s) 73, 436
Bsp119I TTCGAA 1 cut(s) 18
Bsp1286I GDGCHC 1 cut(s) 168
Bsp143I GATC 7 cut(s) 231, 259, 487, 764, 973, 1053, 1106
BspACI CCGC 5 cut(s) 160, 182, 256, 290, 650
BspANI GGCC 2 cut(s) 73, 436
BspDI ATCGAT 2 cut(s) 984, 1056
BspFNI CGCG 2 cut(s) 162, 256
BspLI GGNNCC 2 cut(s) 261, 956
BspMAI CTGCAG 2 cut(s) 1008, 1210
BspPI GGATC 3 cut(s) 226, 254, 267
BspT104I TTCGAA 1 cut(s) 18
BspT107I GGYRCC 1 cut(s) 954
BsrDI GCAATG 3 cut(s) 493, 636, 1150
BsrI ACTGG 2 cut(s) 709, 1133
BssECI CCNNGG 6 cut(s) 362, 500, 644, 645, 793, 884
BssMI GATC 7 cut(s) 231, 259, 487, 764, 973, 1053, 1106
BssNI GRCGYC 1 cut(s) 955
BssT1I CCWWGG 3 cut(s) 362, 500, 884
Bst2UI CCWGG 4 cut(s) 25, 264, 646, 1117
Bst4CI ACNGT 7 cut(s) 220, 322, 352, 473, 619, 794, 1221
Bst6I CTCTTC 2 cut(s) 245, 1020
BstACI GRCGYC 1 cut(s) 955
BstBI TTCGAA 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 164, 425
BstDEI CTNAG 1 cut(s) 196
BstDSI CCRYGG 1 cut(s) 793
BstF5I GGATG 1 cut(s) 286
BstFNI CGCG 2 cut(s) 162, 256
BstH2I RGCGCY 3 cut(s) 484, 958, 1097
BstHHI GCGC 5 cut(s) 256, 483, 957, 1045, 1096
BstKTI GATC 7 cut(s) 234, 262, 490, 767, 976, 1056, 1109
BstMAI GTCTC 1 cut(s) 812
BstMBI GATC 7 cut(s) 231, 259, 487, 764, 973, 1053, 1106
BstMWI GCNNNNNNNGC 1 cut(s) 433
BstNI CCWGG 4 cut(s) 25, 264, 646, 1117
BstNSI RCATGY 3 cut(s) 191, 423, 427
BstSCI CCNGG 4 cut(s) 23, 262, 644, 1115
BstSFI CTRYAG 3 cut(s) 511, 1004, 1206
BstUI CGCG 2 cut(s) 162, 256
BstV1I GCAGC 5 cut(s) 1018, 1021, 1044, 1192, 1214
BstV2I GAAGAC 3 cut(s) 7, 343, 975
BstX2I RGATCY 1 cut(s) 259
BstXI CCANNNNNNTGG 1 cut(s) 81
BstYI RGATCY 1 cut(s) 259
Bsu15I ATCGAT 2 cut(s) 984, 1056
BsuRI GGCC 2 cut(s) 73, 436
BsuTUI ATCGAT 2 cut(s) 984, 1056
BtgI CCRYGG 1 cut(s) 793
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 348
Cac8I GCNNGC 2 cut(s) 164, 425
CfoI GCGC 5 cut(s) 256, 483, 957, 1045, 1096
Cfr13I GGNCC 1 cut(s) 71
ClaI ATCGAT 2 cut(s) 984, 1056
Csp6I GTAC 5 cut(s) 227, 517, 615, 718, 760
CviAII CATG 6 cut(s) 188, 379, 420, 424, 909, 1121
CviQI GTAC 5 cut(s) 227, 517, 615, 718, 760
DdeI CTNAG 1 cut(s) 196
DinI GGCGCC 1 cut(s) 956
DpnI GATC 7 cut(s) 233, 261, 489, 766, 975, 1055, 1108
DpnII GATC 7 cut(s) 231, 259, 487, 764, 973, 1053, 1106
DraI TTTAAA 1 cut(s) 1017
Eam1104I CTCTTC 2 cut(s) 245, 1020
EarI CTCTTC 2 cut(s) 245, 1020
EciI GGCGGA 1 cut(s) 279
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 3 cut(s) 362, 500, 884
Eco24I GRGCYC 1 cut(s) 168
Eco47III AGCGCT 1 cut(s) 482
Eco53kI GAGCTC 1 cut(s) 166
Eco57I CTGAAG 2 cut(s) 841, 1161
EcoICRI GAGCTC 1 cut(s) 166
EcoRII CCWGG 4 cut(s) 23, 262, 644, 1115
EcoT14I CCWWGG 3 cut(s) 362, 500, 884
EcoT22I ATGCAT 1 cut(s) 425
EcoT38I GRGCYC 1 cut(s) 168
EgeI GGCGCC 1 cut(s) 956
EheI GGCGCC 1 cut(s) 956
ErhI CCWWGG 3 cut(s) 362, 500, 884
FaeI CATG 6 cut(s) 191, 382, 423, 427, 912, 1124
FaqI GGGAC 1 cut(s) 198
FatI CATG 6 cut(s) 187, 378, 419, 423, 908, 1120
FauI CCCGC 2 cut(s) 175, 249
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 6 cut(s) 160, 1007, 1010, 1033, 1203, 1206
FokI GGATG 1 cut(s) 273
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 6 cut(s) 160, 1007, 1010, 1033, 1203, 1206
FspBI CTAG 2 cut(s) 363, 977
GlaI GCGC 5 cut(s) 255, 482, 956, 1044, 1095
GluI GCNGC 6 cut(s) 160, 1007, 1010, 1033, 1203, 1206
HaeII RGCGCY 3 cut(s) 484, 958, 1097
HaeIII GGCC 2 cut(s) 73, 436
HapII CCGG 1 cut(s) 757
HhaI GCGC 5 cut(s) 256, 483, 957, 1045, 1096
Hin1I GRCGYC 1 cut(s) 955
Hin1II CATG 6 cut(s) 191, 382, 423, 427, 912, 1124
Hin6I GCGC 5 cut(s) 254, 481, 955, 1043, 1094
HinP1I GCGC 5 cut(s) 254, 481, 955, 1043, 1094
HinfI GANTC 4 cut(s) 267, 638, 777, 1074
HpaII CCGG 1 cut(s) 757
HphI GGTGA 1 cut(s) 633
Hpy188I TCNGA 4 cut(s) 597, 769, 860, 1111
Hpy188III TCNNGA 1 cut(s) 977
HpyAV CCTTC 5 cut(s) 22, 109, 245, 364, 955
HpyCH4III ACNGT 7 cut(s) 220, 322, 352, 473, 619, 794, 1221
HpyCH4IV ACGT 2 cut(s) 783, 1161
HpyCH4V TGCA 8 cut(s) 146, 423, 465, 555, 1006, 1148, 1202, 1208
HpyF10VI GCNNNNNNNGC 1 cut(s) 433
HpyF3I CTNAG 1 cut(s) 196
HpySE526I ACGT 2 cut(s) 783, 1161
Hsp92I GRCGYC 1 cut(s) 955
Hsp92II CATG 6 cut(s) 191, 382, 423, 427, 912, 1124
HspAI GCGC 5 cut(s) 254, 481, 955, 1043, 1094
KasI GGCGCC 1 cut(s) 954
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 7 cut(s) 231, 259, 487, 764, 973, 1053, 1106
LmnI GCTCC 2 cut(s) 452, 1133
Lsp1109I GCAGC 5 cut(s) 1018, 1021, 1044, 1192, 1214
LweI GCATC 5 cut(s) 67, 295, 434, 564, 1054
MaeI CTAG 2 cut(s) 363, 977
MaeII ACGT 2 cut(s) 783, 1161
MalI GATC 7 cut(s) 233, 261, 489, 766, 975, 1055, 1108
MboI GATC 7 cut(s) 231, 259, 487, 764, 973, 1053, 1106
MflI RGATCY 1 cut(s) 259
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 6 cut(s) 280, 357, 751, 808, 1101, 1164
Mly113I GGCGCC 1 cut(s) 955
MmeI TCCRAC 1 cut(s) 673
Mph1103I ATGCAT 1 cut(s) 425
MroXI GAANNNNTTC 1 cut(s) 854
MseI TTAA 3 cut(s) 405, 837, 1016
MspA1I CMGCKG 1 cut(s) 1205
MspI CCGG 1 cut(s) 757
MspR9I CCNGG 4 cut(s) 25, 264, 646, 1117
MvaI CCWGG 4 cut(s) 25, 264, 646, 1117
MvnI CGCG 2 cut(s) 162, 256
MwoI GCNNNNNNNGC 1 cut(s) 433
NarI GGCGCC 1 cut(s) 955
NdeII GATC 7 cut(s) 231, 259, 487, 764, 973, 1053, 1106
NlaIII CATG 6 cut(s) 191, 382, 423, 427, 912, 1124
NlaIV GGNNCC 2 cut(s) 261, 956
NsiI ATGCAT 1 cut(s) 425
NspI RCATGY 3 cut(s) 191, 423, 427
NspV TTCGAA 1 cut(s) 18
PaeI GCATGC 1 cut(s) 427
PasI CCCWGGG 1 cut(s) 645
PciI ACATGT 1 cut(s) 187
PcsI WCGNNNNNNNCGW 1 cut(s) 439
PdmI GAANNNNTTC 1 cut(s) 854
PfeI GAWTC 4 cut(s) 267, 638, 777, 1074
PflMI CCANNNNNTGG 1 cut(s) 964
PfoI TCCNGGA 1 cut(s) 262
PkrI GCNGC 6 cut(s) 161, 1008, 1011, 1034, 1204, 1207
PluTI GGCGCC 1 cut(s) 958
PscI ACATGT 1 cut(s) 187
PsiI TTATAA 1 cut(s) 789
Psp124BI GAGCTC 1 cut(s) 168
Psp1406I AACGTT 1 cut(s) 783
Psp6I CCWGG 4 cut(s) 23, 262, 644, 1115
PspGI CCWGG 4 cut(s) 23, 262, 644, 1115
PspN4I GGNNCC 2 cut(s) 261, 956
PspPI GGNCC 1 cut(s) 71
PstI CTGCAG 2 cut(s) 1008, 1210
PsuI RGATCY 1 cut(s) 259
PvuII CAGCTG 1 cut(s) 1205
RsaI GTAC 5 cut(s) 228, 518, 616, 719, 761
RsaNI GTAC 5 cut(s) 227, 517, 615, 718, 760
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 3 cut(s) 405, 837, 1016
SatI GCNGC 6 cut(s) 160, 1007, 1010, 1033, 1203, 1206
Sau3AI GATC 7 cut(s) 231, 259, 487, 764, 973, 1053, 1106
Sau96I GGNCC 1 cut(s) 71
ScrFI CCNGG 4 cut(s) 25, 264, 646, 1117
SduI GDGCHC 1 cut(s) 168
SfaNI GCATC 5 cut(s) 67, 295, 434, 564, 1054
SfcI CTRYAG 3 cut(s) 511, 1004, 1206
SfoI GGCGCC 1 cut(s) 956
SfuI TTCGAA 1 cut(s) 18
SmlI CTYRAG 1 cut(s) 844
SmoI CTYRAG 1 cut(s) 844
SphI GCATGC 1 cut(s) 427
Sse9I AATT 6 cut(s) 280, 357, 751, 808, 1101, 1164
SsiI CCGC 5 cut(s) 160, 182, 256, 290, 650
SspDI GGCGCC 1 cut(s) 954
SspI AATATT 2 cut(s) 410, 892
SspMI CTAG 2 cut(s) 363, 977
SstI GAGCTC 1 cut(s) 168
StyD4I CCNGG 4 cut(s) 23, 262, 644, 1115
StyI CCWWGG 3 cut(s) 362, 500, 884
TaaI ACNGT 7 cut(s) 220, 322, 352, 473, 619, 794, 1221
TaiI ACGT 2 cut(s) 786, 1164
TaqI TCGA 6 cut(s) 18, 442, 569, 984, 1056, 1105
TasI AATT 6 cut(s) 280, 357, 751, 808, 1101, 1164
TatI WGTACW 1 cut(s) 226
TauI GCSGC 1 cut(s) 162
TfiI GAWTC 4 cut(s) 267, 638, 777, 1074
Tru1I TTAA 3 cut(s) 405, 837, 1016
Tru9I TTAA 3 cut(s) 405, 837, 1016
TscAI CASTG 1 cut(s) 355
TseI GCWGC 5 cut(s) 1006, 1009, 1032, 1202, 1205
TspDTI ATGAA 4 cut(s) 856, 948, 1002, 1013
TspRI CASTG 1 cut(s) 355
Van91I CCANNNNNTGG 1 cut(s) 964
XapI RAATTY 3 cut(s) 280, 357, 751
XbaI TCTAGA 1 cut(s) 976
XceI RCATGY 3 cut(s) 191, 423, 427
XmaJI CCTAGG 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 854
XspI CTAG 2 cut(s) 363, 977
Zsp2I ATGCAT 1 cut(s) 425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.