MD10G1032800.v1.1

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010663
Physical Location & Seq
Reverse (-)
4244939 .. 4250872
5934 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1032800.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGGCAGCAAAGCGCGTGTACGAAGTCTGGAAAGGAAGTAATAAGTTCATCCTTGGTGGAAGGTTGATATTTGGGCCAGATGCTAGGTCACTGCTTGTCACATTACTATTAATCATTGCTCCGGTTATTATCTTCTGTGTATTTGTCGCATGGCATCTCCGGCATGAATTTTCTTCATATAATGCTGGCTATGCAATTCTGGTTGTAGCAATTGTATTTACTATCTATGTGTTGGTGCTTCTTTTTCTTACTTCAGCCCGGGACCCAGGGATTATTCCAAGAAATGCACATCCACCAGAGGAAGAGTTCCGTTATGACTCTTCAGTGTCCATTGATGTTGGTGGAAGACAAGTGGCATCTAATGCTGGTGGTAGACAAACTCCAAGCCTTCAATTCCCCAGAACAAAAGAAGTGACGGTCAATGGCATTCCTGTGAGAGTGAAATATTGTGATACTTGTATGCTATATCGTCCGCCTCGTTGCTCTCATTGTTCTATTTGCAACAATTGTGTGGAGCGCTTTGATCACCACTGCCCTTGGGTGGGCCAATGCATTGGACAGTGCAATTATCGTTACTTCTTTCTCTTCGTTTCTTCTGCAACTCTGCTGTGCATCTATGTATTCTCATTATCGGCTGTGTACATCAAGATTCTGATGGATGATCACAGGACAGTTTGGAAGGCAATGCAAGAATCTCCTGCATCTGTGATTCTAATGGCCTACTGTTTCATTTCTCTTTGGTTTGTTGGTGGACTAACCGGCTTCCATTTGTACCTTATTGGTACCAACCAGACTACGTATGAAAACTTCCGGTACAGAGCTGACAATAGGATCAATGTTTACAACCGGGGCTGTTTAAATAACTTTCTCGAAGTGTTTTGCACACAAGTAAAACCCTCTAGAAATAACTTTCGAGCTTTCGTTGAAGAGGAGGTACAAAGGCCTCCCCCTATGCCCATTGCACGGGAAGCAGAACCTGATGAATCTGGTGGGGATCCACGTTCAAAGGTTGAGGATGATTTAGATATTGGTGAAGATCTGTTGAAGATCTCACAGCGTCGTAACATTGAAGAAATTGATGAGGACATACGCAGTAGAGGGAGCAACGGACCTCATAATACCTCAGAGGTGCTCTCTGCTTTAGGTTCAGACCGGGCTCCCACTATTCGATCTGATGCTAGGCACTCGAGTTGGGGAAGAAGTGGGAGTTGGGAAATTGCACCGGACGTCCTTGCTAATGTCAATATCACTGAAAGTAGAGGTCATGTGACGTCGAAGGATGCAGTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
ko04391 map04391
Pfam Domains
Protein Families
DHHC

Protein Analysis

431

Amino Acids

48.54

Weight (kDa)

7.13

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 148 - 271 1e-38 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24630 AT5G50020 AT5G50020 AT5G50020
fragaria_vesca FvH4_2g13200
malus_domestica MD10G1032800.v1.1
prunus_persica Prupe.8G038400_v2.0.a1 Prupe.8G038400_v2.0.a1 Prupe.8G038400_v2.0.a1
pyrus_communis pycom10g02090
rosa_chinensis RchiOBHm_Chr6g0274491
rosa_laevigata RLG00000013537
rosa_multiflora Rmu_sc0001289.1_g000020
rosa_roxburghii Rroxscaffold_7G00194180
rosa_rugosa Rorug06G0082200
rosa_samantha Rh6AG198500 Rh6BG201800 Rh6CG202600 Rh6DG193100
rosa_wichuraiana Rw6G017260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1230, 1274
Acc65I GGTACC 1 cut(s) 782
AccB1I GGYRCC 1 cut(s) 782
AccI GTMKAC 1 cut(s) 373
AccII CGCG 1 cut(s) 15
AciI CCGC 1 cut(s) 473
AclWI GGATC 3 cut(s) 839, 989, 1002
AcsI RAATTY 1 cut(s) 167
AcuI CTGAAG 2 cut(s) 237, 306
AcyI GRCGYC 2 cut(s) 1227, 1271
AfaI GTAC 6 cut(s) 20, 641, 773, 784, 815, 936
AfeI AGCGCT 1 cut(s) 518
AfiI CCNNNNNNNGG 3 cut(s) 541, 542, 963
AgsI TTSAA 6 cut(s) 392, 926, 1005, 1045, 1070, 1289
AjnI CCWGG 1 cut(s) 265
AjuI GAANNNNNNNTTGG 2 cut(s) 1192, 1224
AluBI AGCT 2 cut(s) 821, 917
AluI AGCT 2 cut(s) 821, 917
Alw21I GWGCWC 1 cut(s) 1134
AlwI GGATC 3 cut(s) 839, 989, 1002
AlwNI CAGNNNCTG 1 cut(s) 977
Ama87I CYCGRG 2 cut(s) 258, 1186
Aor51HI AGCGCT 1 cut(s) 518
AoxI GGCC 4 cut(s) 74, 544, 717, 941
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 167
AseI ATTAAT 1 cut(s) 110
Asp718I GGTACC 1 cut(s) 782
AspLEI GCGC 2 cut(s) 15, 519
AspS9I GGNCC 4 cut(s) 74, 262, 544, 1109
AsuC2I CCSGG 4 cut(s) 259, 260, 848, 1154
AsuHPI GGTGA 2 cut(s) 518, 1043
AvaI CYCGRG 2 cut(s) 258, 1186
AvaII GGWCC 2 cut(s) 262, 1109
BamHI GGATCC 1 cut(s) 994
BanI GGYRCC 1 cut(s) 782
BanII GRGCYC 1 cut(s) 1159
BarI GAAGNNNNNNTAC 4 cut(s) 791, 823, 918, 950
BbsI GAAGAC 1 cut(s) 352
Bbv12I GWGCWC 1 cut(s) 1134
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 649
BciT130I CCWGG 1 cut(s) 267
BclI TGATCA 2 cut(s) 523, 661
BcnI CCSGG 4 cut(s) 259, 260, 848, 1154
BfaI CTAG 3 cut(s) 84, 900, 1179
BfoI RGCGCY 1 cut(s) 520
BglII AGATCT 2 cut(s) 1036, 1047
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 5 cut(s) 259, 260, 267, 848, 1154
Bme18I GGWCC 2 cut(s) 262, 1109
BmeT110I CYCGRG 2 cut(s) 258, 1186
BmgT120I GGNCC 4 cut(s) 74, 262, 544, 1109
BmiI GGNNCC 5 cut(s) 263, 264, 784, 996, 1158
BmrFI CCNGG 5 cut(s) 259, 260, 267, 848, 1154
BmsI GCATC 7 cut(s) 70, 163, 365, 621, 710, 1165, 1270
BpiI GAAGAC 1 cut(s) 352
BplI GAGNNNNNCTC 2 cut(s) 1118, 1150
BpuMI CCSGG 4 cut(s) 259, 260, 848, 1154
BsaAI YACGTR 1 cut(s) 798
BsaHI GRCGYC 2 cut(s) 1227, 1271
BsaJI CCNNGG 6 cut(s) 52, 258, 265, 266, 536, 847
BsaWI WCCGGW 3 cut(s) 121, 810, 1222
Bsc4I CCNNNNNNNGG 3 cut(s) 541, 542, 963
Bse118I RCCGGY 1 cut(s) 758
Bse3DI GCAATG 3 cut(s) 114, 690, 957
BseBI CCWGG 1 cut(s) 267
BseDI CCNNGG 6 cut(s) 52, 258, 265, 266, 536, 847
BseGI GGATG 5 cut(s) 48, 289, 664, 1021, 1285
BseLI CCNNNNNNNGG 3 cut(s) 541, 542, 963
BseMI GCAATG 3 cut(s) 114, 690, 957
BseMII CTCAG 1 cut(s) 1137
BseRI GAGGAG 1 cut(s) 944
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 15
BshFI GGCC 4 cut(s) 76, 546, 719, 943
BshNI GGYRCC 1 cut(s) 782
BsiHKAI GWGCWC 1 cut(s) 1134
BsiHKCI CYCGRG 2 cut(s) 258, 1186
BsiSI CCGG 8 cut(s) 122, 160, 259, 759, 811, 847, 1153, 1223
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 3 cut(s) 541, 542, 963
BsmFI GGGAC 1 cut(s) 275
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 4 cut(s) 76, 546, 719, 943
BsoBI CYCGRG 2 cut(s) 258, 1186
Bsp1286I GDGCHC 2 cut(s) 1134, 1159
Bsp1407I TGTACA 1 cut(s) 639
Bsp143I GATC 7 cut(s) 523, 661, 831, 994, 1036, 1047, 1169
BspACI CCGC 1 cut(s) 473
BspANI GGCC 4 cut(s) 76, 546, 719, 943
BspCNI CTCAG 1 cut(s) 1136
BspFNI CGCG 1 cut(s) 15
BspLI GGNNCC 5 cut(s) 263, 264, 784, 996, 1158
BspPI GGATC 3 cut(s) 839, 989, 1002
BspT107I GGYRCC 1 cut(s) 782
BsrDI GCAATG 3 cut(s) 114, 690, 957
BsrFI RCCGGY 1 cut(s) 758
BsrGI TGTACA 1 cut(s) 639
BssAI RCCGGY 1 cut(s) 758
BssECI CCNNGG 6 cut(s) 52, 258, 265, 266, 536, 847
BssMI GATC 7 cut(s) 523, 661, 831, 994, 1036, 1047, 1169
BssNI GRCGYC 2 cut(s) 1227, 1271
BssT1I CCWWGG 2 cut(s) 52, 536
Bst2UI CCWGG 1 cut(s) 267
Bst4CI ACNGT 4 cut(s) 418, 561, 673, 725
Bst6I CTCTTC 4 cut(s) 297, 325, 590, 921
BstACI GRCGYC 2 cut(s) 1227, 1271
BstAPI GCANNNNNTGC 1 cut(s) 362
BstAUI TGTACA 1 cut(s) 639
BstBAI YACGTR 1 cut(s) 798
BstC8I GCNNGC 1 cut(s) 187
BstDEI CTNAG 1 cut(s) 1123
BstF5I GGATG 5 cut(s) 48, 289, 664, 1021, 1285
BstFNI CGCG 1 cut(s) 15
BstH2I RGCGCY 1 cut(s) 520
BstHHI GCGC 2 cut(s) 15, 519
BstKTI GATC 7 cut(s) 526, 664, 834, 997, 1039, 1050, 1172
BstMBI GATC 7 cut(s) 523, 661, 831, 994, 1036, 1047, 1169
BstMWI GCNNNNNNNGC 4 cut(s) 160, 191, 362, 968
BstNI CCWGG 1 cut(s) 267
BstSCI CCNGG 5 cut(s) 257, 258, 265, 846, 1152
BstSNI TACGTA 1 cut(s) 798
BstUI CGCG 1 cut(s) 15
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 352
BstX2I RGATCY 3 cut(s) 994, 1036, 1047
BstXI CCANNNNNNTGG 1 cut(s) 554
BstYI RGATCY 3 cut(s) 994, 1036, 1047
BsuRI GGCC 4 cut(s) 76, 546, 719, 943
BtsCI GGATG 5 cut(s) 48, 289, 664, 1021, 1285
BtsI GCAGTG 2 cut(s) 89, 529
BtsIMutI CAGTG 5 cut(s) 89, 330, 529, 566, 1248
Cac8I GCNNGC 1 cut(s) 187
CaiI CAGNNNCTG 1 cut(s) 977
CfoI GCGC 2 cut(s) 15, 519
Cfr10I RCCGGY 1 cut(s) 758
Cfr13I GGNCC 4 cut(s) 74, 262, 544, 1109
Cfr9I CCCGGG 1 cut(s) 258
CseI GACGC 1 cut(s) 1046
Csp6I GTAC 6 cut(s) 19, 640, 772, 783, 814, 935
CspCI CAANNNNNGTGG 2 cut(s) 518, 553
CviAII CATG 3 cut(s) 150, 164, 1265
CviQI GTAC 6 cut(s) 19, 640, 772, 783, 814, 935
DdeI CTNAG 1 cut(s) 1123
DpnI GATC 7 cut(s) 525, 663, 833, 996, 1038, 1049, 1171
DpnII GATC 7 cut(s) 523, 661, 831, 994, 1036, 1047, 1169
DraI TTTAAA 1 cut(s) 858
Eam1104I CTCTTC 4 cut(s) 297, 325, 590, 921
EarI CTCTTC 4 cut(s) 297, 325, 590, 921
EciI GGCGGA 1 cut(s) 462
Eco105I TACGTA 1 cut(s) 798
Eco130I CCWWGG 2 cut(s) 52, 536
Eco147I AGGCCT 1 cut(s) 943
Eco24I GRGCYC 1 cut(s) 1159
Eco47I GGWCC 2 cut(s) 262, 1109
Eco47III AGCGCT 1 cut(s) 518
Eco57I CTGAAG 2 cut(s) 237, 306
Eco88I CYCGRG 2 cut(s) 258, 1186
EcoO109I RGGNCCY 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 265
EcoT14I CCWWGG 2 cut(s) 52, 536
EcoT22I ATGCAT 1 cut(s) 554
EcoT38I GRGCYC 1 cut(s) 1159
ErhI CCWWGG 2 cut(s) 52, 536
FaeI CATG 3 cut(s) 153, 167, 1268
FaqI GGGAC 1 cut(s) 275
FatI CATG 3 cut(s) 149, 163, 1264
FbaI TGATCA 2 cut(s) 523, 661
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 4 cut(s) 35, 276, 671, 1028
FriOI GRGCYC 1 cut(s) 1159
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 3 cut(s) 84, 900, 1179
GlaI GCGC 2 cut(s) 14, 518
GluI GCNGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 520
HaeIII GGCC 4 cut(s) 76, 546, 719, 943
HapII CCGG 8 cut(s) 122, 160, 259, 759, 811, 847, 1153, 1223
HgaI GACGC 1 cut(s) 1046
HhaI GCGC 2 cut(s) 15, 519
Hin1I GRCGYC 2 cut(s) 1227, 1271
Hin1II CATG 3 cut(s) 153, 167, 1268
Hin6I GCGC 2 cut(s) 13, 517
HinP1I GCGC 2 cut(s) 13, 517
HinfI GANTC 5 cut(s) 317, 649, 692, 709, 983
HpaII CCGG 8 cut(s) 122, 160, 259, 759, 811, 847, 1153, 1223
HphI GGTGA 2 cut(s) 518, 1043
Hpy166II GTNNAC 5 cut(s) 19, 374, 640, 752, 841
Hpy188I TCNGA 4 cut(s) 654, 1126, 1150, 1174
Hpy188III TCNNGA 4 cut(s) 28, 646, 869, 900
Hpy8I GTNNAC 5 cut(s) 19, 374, 640, 752, 841
Hpy99I CGWCG 2 cut(s) 1062, 1276
HpyAV CCTTC 4 cut(s) 54, 398, 673, 1270
HpyCH4III ACNGT 4 cut(s) 418, 561, 673, 725
HpyCH4IV ACGT 4 cut(s) 797, 1000, 1227, 1271
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 191, 362, 968
HpyF3I CTNAG 1 cut(s) 1123
HpySE526I ACGT 4 cut(s) 797, 1000, 1227, 1271
Hsp92I GRCGYC 2 cut(s) 1227, 1271
Hsp92II CATG 3 cut(s) 153, 167, 1268
HspAI GCGC 2 cut(s) 13, 517
KflI GGGWCCC 1 cut(s) 262
KpnI GGTACC 1 cut(s) 786
Ksp22I TGATCA 2 cut(s) 523, 661
Kzo9I GATC 7 cut(s) 523, 661, 831, 994, 1036, 1047, 1169
LmnI GCTCC 4 cut(s) 124, 514, 1101, 1162
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 7 cut(s) 70, 163, 365, 621, 710, 1165, 1270
MaeI CTAG 3 cut(s) 84, 900, 1179
MaeII ACGT 4 cut(s) 797, 1000, 1227, 1271
MaeIII GTNAC 6 cut(s) 87, 97, 412, 572, 1061, 1267
MalI GATC 7 cut(s) 525, 663, 833, 996, 1038, 1049, 1171
MboI GATC 7 cut(s) 523, 661, 831, 994, 1036, 1047, 1169
MfeI CAATTG 2 cut(s) 210, 505
MflI RGATCY 3 cut(s) 994, 1036, 1047
MhlI GDGCHC 2 cut(s) 1134, 1159
MluCI AATT 8 cut(s) 167, 195, 210, 392, 505, 565, 1074, 1215
MlyI GAGTC 1 cut(s) 311
Mph1103I ATGCAT 1 cut(s) 554
MseI TTAA 2 cut(s) 110, 857
MslI CAYNNNNRTG 1 cut(s) 431
MspI CCGG 8 cut(s) 122, 160, 259, 759, 811, 847, 1153, 1223
MspR9I CCNGG 5 cut(s) 259, 260, 267, 848, 1154
MunI CAATTG 2 cut(s) 210, 505
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 1 cut(s) 267
MvnI CGCG 1 cut(s) 15
MwoI GCNNNNNNNGC 4 cut(s) 160, 191, 362, 968
NciI CCSGG 4 cut(s) 259, 260, 848, 1154
NdeII GATC 7 cut(s) 523, 661, 831, 994, 1036, 1047, 1169
NlaIII CATG 3 cut(s) 153, 167, 1268
NlaIV GGNNCC 5 cut(s) 263, 264, 784, 996, 1158
NmuCI GTSAC 4 cut(s) 87, 97, 412, 1267
NsiI ATGCAT 1 cut(s) 554
PaeR7I CTCGAG 1 cut(s) 1186
PasI CCCWGGG 1 cut(s) 266
PceI AGGCCT 1 cut(s) 943
PcsI WCGNNNNNNNCGW 1 cut(s) 475
PctI GAATGC 1 cut(s) 426
PfeI GAWTC 4 cut(s) 649, 692, 709, 983
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
Ppu21I YACGTR 1 cut(s) 798
PpuMI RGGWCCY 1 cut(s) 262
PshBI ATTAAT 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 265
PspN4I GGNNCC 5 cut(s) 263, 264, 784, 996, 1158
PspPI GGNCC 4 cut(s) 74, 262, 544, 1109
PspPPI RGGWCCY 1 cut(s) 262
PspXI VCTCGAGB 1 cut(s) 1186
PstNI CAGNNNCTG 1 cut(s) 977
PsuI RGATCY 3 cut(s) 994, 1036, 1047
RsaI GTAC 6 cut(s) 20, 641, 773, 784, 815, 936
RsaNI GTAC 6 cut(s) 19, 640, 772, 783, 814, 935
RseI CAYNNNNRTG 1 cut(s) 431
SaqAI TTAA 2 cut(s) 110, 857
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 7 cut(s) 523, 661, 831, 994, 1036, 1047, 1169
Sau96I GGNCC 4 cut(s) 74, 262, 544, 1109
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 5 cut(s) 259, 260, 267, 848, 1154
SduI GDGCHC 2 cut(s) 1134, 1159
SfaNI GCATC 7 cut(s) 70, 163, 365, 621, 710, 1165, 1270
Sfr274I CTCGAG 1 cut(s) 1186
SinI GGWCC 2 cut(s) 262, 1109
SlaI CTCGAG 1 cut(s) 1186
SmaI CCCGGG 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 431
SmlI CTYRAG 1 cut(s) 1186
SmoI CTYRAG 1 cut(s) 1186
SnaBI TACGTA 1 cut(s) 798
Sse9I AATT 8 cut(s) 167, 195, 210, 392, 505, 565, 1074, 1215
SseBI AGGCCT 1 cut(s) 943
SsiI CCGC 1 cut(s) 473
SspI AATATT 1 cut(s) 446
SspMI CTAG 3 cut(s) 84, 900, 1179
StuI AGGCCT 1 cut(s) 943
StyD4I CCNGG 5 cut(s) 257, 258, 265, 846, 1152
StyI CCWWGG 2 cut(s) 52, 536
TaaI ACNGT 4 cut(s) 418, 561, 673, 725
TaiI ACGT 4 cut(s) 800, 1003, 1230, 1274
TaqI TCGA 5 cut(s) 870, 913, 1168, 1187, 1274
TasI AATT 8 cut(s) 167, 195, 210, 392, 505, 565, 1074, 1215
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 4 cut(s) 649, 692, 709, 983
Tru1I TTAA 2 cut(s) 110, 857
Tru9I TTAA 2 cut(s) 110, 857
TscAI CASTG 5 cut(s) 96, 330, 536, 566, 1255
TseFI GTSAC 4 cut(s) 87, 97, 412, 1267
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 4 cut(s) 87, 97, 412, 1267
TspDTI ATGAA 6 cut(s) 37, 165, 180, 718, 816, 996
TspGWI ACGGA 2 cut(s) 299, 1122
TspMI CCCGGG 1 cut(s) 258
TspRI CASTG 5 cut(s) 96, 330, 536, 566, 1255
VpaK11BI GGWCC 2 cut(s) 262, 1109
VspI ATTAAT 1 cut(s) 110
XapI RAATTY 1 cut(s) 167
XbaI TCTAGA 1 cut(s) 899
XhoI CTCGAG 1 cut(s) 1186
XmaI CCCGGG 1 cut(s) 258
XmiI GTMKAC 1 cut(s) 373
XspI CTAG 3 cut(s) 84, 900, 1179
ZraI GACGTC 2 cut(s) 1228, 1272
Zsp2I ATGCAT 1 cut(s) 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.