AT5G52180

Predicted transmembrane protein 161AB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016225
Physical Location & Seq
Reverse (-)
21200040 .. 21201610
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52180.1

Sequence Viewer

Length: 1377 bp
ATGCTTGAGCTTTTAGGAAGTTACAGGAATCTAACACTTCAAATCCTACTATCTCTCTTCTTAACGTTAGTCCTTTCATTCGTCAAAATCTCAATCATCTTCCTCCACGGTCTCTGTACCTATATCCAACCTGAAAATCTCAACCAAAACAACACCGGAAATGGACTCCGACCTGCGATTAGACGACCATCTAGTACCGACGATCCAAAATCTAATGTTGAAGTCAGAAGAAGAAACAGATCCAAAGATAAATCCGAATTCGACGAAAGCAATGCTCAGATCTTTCGAATTAAGCTCGATGAAGATCATCTCAGATCTCGAATGTATTTCACTGAGTACAATTCGCTATTCGTGATATCGTTCTTAGCTGTTTCTTGCTTTCTTCTAGATAAGTACTTCGGTATTATTGAGGATAATAGTCATGGAGTTTTAAGTAATGGATTGATGTTTCCGATTGTTCTAGGGTTTATAGCTTTGTGTAAGGTGTTTGTAGCTTTGGGGAAGATTTCTATTGAGAGATCTGCTTCTAAGGAATCTGAGAAGAGATTGAGTTTGATTTTTGGTGTTTTAGGGTTTGTGTTTGGGATTATAATCAGTGCTGGTGTTTTTCCTAAAGGATTTGATTTTCAATTAGGTTCTGTTGATGCGTTTTGTTGCATTGTGATATCGTTTTCGATGGCTTGTATTGGTGGTTTCCTTTATATGCCTGCTGGAAGAAGTGCAAGGTCGTTTTGGGTTGGGACTGATCAGATTAGGAGTAATTTGTCGATCATTTCTTGTGGATGGTTTGGTAGGATGATTCTATATGCGAATTATATCGTATCGGTTTTCACTTCGTTGCTTTGGATTCATCCGTTGGCAGAGGTTTTAGTGAAGAGAAGTGGTGATCCTGGGACTAGTGGTAGTAGTCAGAGTCAGAGTGGGTTGGTTGGTAATGTAGGCATGTTGAGTGATGATTTTGCGAAGTTTCGGGTTTTGTGTTTGTTACTTTCGGGTTTACTACAAGCGATGGCGGTTCGTCCTAACCTTCAGATGTTCTTGAACGAAGCAGTCCTTTCGTGGTATCAGAGATTACATGGAAGTAAAACTCCTGATTTGGATTTTAGTCGTGCAAAGATGTTTCTTCACAACCATTACCTATGCCTTGTTGCTCTGCAGTTTTTGGCGCCTTCGGTGCTAGTGATTCTTTTCCTCGGCTTGTCTCAGATTGACTTGAGCTCTTTTGCTGTATCTCAACTGGTATGTGGTTCTTTGCCTTGTAGCGATTTCATTAAACAAGTTGGTTTGTTGATGTCTTGGTGGGTTCTTTTTGTTTGGTCGGGTTTTACTTCAGCGAGTCTTGTTTTCTATCGTCGAGGTGTCTTGTATGTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_161AB

Protein Analysis

458

Amino Acids

51.11

Weight (kDa)

9.0

Isoelectric Point (pI)

49.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_161AB PF10268 206 - 397 8.6e-12 Predicted transmembrane protein 161AB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52180
fragaria_vesca FvH4_7g29210
malus_domestica MD01G1198800.v1.1 MD07G1266600.v1.1
prunus_persica Prupe.2G292200_v2.0.a1
pyrus_communis pycom07g24440
rosa_chinensis RchiOBHm_Chr1g0376971
rosa_laevigata RLG00000026555
rosa_roxburghii Rroxscaffold_4G00281600
rosa_rugosa Rorug01G0400000
rosa_samantha Rh1AG417200 Rh1BG376600 Rh1CG390400 Rh1DG407300
rosa_wichuraiana Rw1G036620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 590
Acc36I ACCTGC 1 cut(s) 181
AccB1I GGYRCC 1 cut(s) 1165
AciI CCGC 1 cut(s) 1013
AclI AACGTT 1 cut(s) 65
AclWI GGATC 3 cut(s) 197, 234, 881
AcsI RAATTY 1 cut(s) 257
AcuI CTGAAG 2 cut(s) 1013, 1314
AcyI GRCGYC 1 cut(s) 1166
AfaI GTAC 4 cut(s) 118, 196, 338, 395
AgsI TTSAA 4 cut(s) 41, 221, 629, 1042
AhlI ACTAGT 1 cut(s) 896
AjnI CCWGG 1 cut(s) 889
AluBI AGCT 6 cut(s) 10, 295, 368, 473, 494, 1218
AluI AGCT 6 cut(s) 10, 295, 368, 473, 494, 1218
Alw21I GWGCWC 1 cut(s) 1220
Alw26I GTCTC 2 cut(s) 116, 1206
AlwI GGATC 3 cut(s) 197, 234, 881
ApoI RAATTY 1 cut(s) 257
AspLEI GCGC 1 cut(s) 1168
AsuHPI GGTGA 1 cut(s) 896
AsuII TTCGAA 1 cut(s) 286
BanI GGYRCC 1 cut(s) 1165
BanII GRGCYC 1 cut(s) 1220
Bbv12I GWGCWC 1 cut(s) 1220
BccI CCATC 4 cut(s) 196, 670, 777, 1003
BcgI CGANNNNNNTGC 4 cut(s) 254, 288, 1038, 1072
BciT130I CCWGG 1 cut(s) 891
BclI TGATCA 1 cut(s) 745
BcoDI GTCTC 2 cut(s) 116, 1206
BcuI ACTAGT 1 cut(s) 896
BfaI CTAG 5 cut(s) 192, 386, 461, 897, 1178
BfmI CTRYAG 1 cut(s) 1154
BfoI RGCGCY 1 cut(s) 1169
BfuAI ACCTGC 1 cut(s) 181
BglII AGATCT 3 cut(s) 279, 314, 518
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 1 cut(s) 891
BmiI GGNNCC 1 cut(s) 1167
BmrFI CCNGG 1 cut(s) 891
BmsI GCATC 1 cut(s) 634
Bpu14I TTCGAA 1 cut(s) 286
BpuEI CTTGAG 2 cut(s) 26, 1234
BsaBI GATNNNNATC 2 cut(s) 303, 590
BsaHI GRCGYC 1 cut(s) 1166
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 3 cut(s) 106, 890, 1192
BsaWI WCCGGW 1 cut(s) 155
Bse1I ACTGG 1 cut(s) 1242
Bse3DI GCAATG 1 cut(s) 277
Bse8I GATNNNNATC 2 cut(s) 303, 590
BseBI CCWGG 1 cut(s) 891
BseDI CCNNGG 3 cut(s) 106, 890, 1192
BseGI GGATG 3 cut(s) 788, 801, 850
BseJI GATNNNNATC 2 cut(s) 303, 590
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 5 cut(s) 290, 324, 325, 528, 1217
BseNI ACTGG 1 cut(s) 1242
BshNI GGYRCC 1 cut(s) 1165
BsiHKAI GWGCWC 1 cut(s) 1220
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 2 cut(s) 754, 907
BsmAI GTCTC 2 cut(s) 116, 1206
BsmFI GGGAC 2 cut(s) 754, 907
Bso31I GGTCTC 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 286
Bsp1286I GDGCHC 1 cut(s) 1220
Bsp143I GATC 9 cut(s) 202, 239, 279, 304, 314, 518, 745, 768, 886
BspACI CCGC 1 cut(s) 1013
BspCNI CTCAG 5 cut(s) 289, 324, 325, 529, 1216
BspLI GGNNCC 1 cut(s) 1167
BspMAI CTGCAG 1 cut(s) 1158
BspMI ACCTGC 1 cut(s) 181
BspPI GGATC 3 cut(s) 197, 234, 881
BspT104I TTCGAA 1 cut(s) 286
BspT107I GGYRCC 1 cut(s) 1165
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 277
BsrI ACTGG 1 cut(s) 1242
BssECI CCNNGG 3 cut(s) 106, 890, 1192
BssMI GATC 9 cut(s) 202, 239, 279, 304, 314, 518, 745, 768, 886
BssNI GRCGYC 1 cut(s) 1166
Bst2UI CCWGG 1 cut(s) 891
Bst4CI ACNGT 1 cut(s) 110
Bst6I CTCTTC 3 cut(s) 62, 536, 869
BstACI GRCGYC 1 cut(s) 1166
BstBI TTCGAA 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 708
BstDEI CTNAG 7 cut(s) 276, 311, 333, 364, 528, 537, 1203
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 3 cut(s) 788, 801, 850
BstH2I RGCGCY 1 cut(s) 1169
BstHHI GCGC 1 cut(s) 1168
BstKTI GATC 9 cut(s) 205, 242, 282, 307, 317, 521, 748, 771, 889
BstMAI GTCTC 2 cut(s) 116, 1206
BstMBI GATC 9 cut(s) 202, 239, 279, 304, 314, 518, 745, 768, 886
BstMWI GCNNNNNNNGC 1 cut(s) 1174
BstNI CCWGG 1 cut(s) 891
BstNSI RCATGY 1 cut(s) 946
BstSCI CCNGG 1 cut(s) 889
BstSFI CTRYAG 1 cut(s) 1154
BstX2I RGATCY 4 cut(s) 239, 279, 314, 518
BstYI RGATCY 4 cut(s) 239, 279, 314, 518
BtgI CCRYGG 1 cut(s) 106
BtgZI GCGATG 1 cut(s) 1022
BtsCI GGATG 3 cut(s) 788, 801, 850
BtsIMutI CAGTG 2 cut(s) 330, 601
BveI ACCTGC 1 cut(s) 181
Cac8I GCNNGC 1 cut(s) 708
CfoI GCGC 1 cut(s) 1168
Csp6I GTAC 4 cut(s) 117, 195, 337, 394
CviAII CATG 3 cut(s) 422, 943, 1076
CviJI RGCY 8 cut(s) 10, 295, 368, 473, 494, 680, 1197, 1218
CviKI_1 RGCY 8 cut(s) 10, 295, 368, 473, 494, 680, 1197, 1218
CviQI GTAC 4 cut(s) 117, 195, 337, 394
DdeI CTNAG 7 cut(s) 276, 311, 333, 364, 528, 537, 1203
DinI GGCGCC 1 cut(s) 1167
DpnI GATC 9 cut(s) 204, 241, 281, 306, 316, 520, 747, 770, 888
DpnII GATC 9 cut(s) 202, 239, 279, 304, 314, 518, 745, 768, 886
Eam1104I CTCTTC 3 cut(s) 62, 536, 869
EarI CTCTTC 3 cut(s) 62, 536, 869
Ecl136II GAGCTC 1 cut(s) 1218
Eco24I GRGCYC 1 cut(s) 1220
Eco31I GGTCTC 1 cut(s) 116
Eco32I GATATC 2 cut(s) 357, 666
Eco53kI GAGCTC 1 cut(s) 1218
Eco57I CTGAAG 2 cut(s) 1013, 1314
EcoICRI GAGCTC 1 cut(s) 1218
EcoRI GAATTC 1 cut(s) 257
EcoRII CCWGG 1 cut(s) 889
EcoRV GATATC 2 cut(s) 357, 666
EcoT38I GRGCYC 1 cut(s) 1220
EgeI GGCGCC 1 cut(s) 1167
EheI GGCGCC 1 cut(s) 1167
FaeI CATG 3 cut(s) 425, 946, 1079
FalI AAGNNNNNCTT 2 cut(s) 1038, 1070
FaqI GGGAC 2 cut(s) 754, 907
FatI CATG 3 cut(s) 421, 942, 1075
FbaI TGATCA 1 cut(s) 745
FokI GGATG 3 cut(s) 795, 808, 837
FriOI GRGCYC 1 cut(s) 1220
FspBI CTAG 5 cut(s) 192, 386, 461, 897, 1178
GlaI GCGC 1 cut(s) 1167
HaeII RGCGCY 1 cut(s) 1169
HapII CCGG 1 cut(s) 156
HhaI GCGC 1 cut(s) 1168
Hin1I GRCGYC 1 cut(s) 1166
Hin1II CATG 3 cut(s) 425, 946, 1079
Hin6I GCGC 1 cut(s) 1166
HinP1I GCGC 1 cut(s) 1166
HinfI GANTC 8 cut(s) 28, 165, 533, 799, 847, 913, 1183, 1336
HpaII CCGG 1 cut(s) 156
HphI GGTGA 1 cut(s) 896
Hpy166II GTNNAC 1 cut(s) 998
Hpy188III TCNNGA 6 cut(s) 318, 352, 386, 1039, 1091, 1374
Hpy8I GTNNAC 1 cut(s) 998
Hpy99I CGWCG 3 cut(s) 203, 266, 1356
HpyAV CCTTC 2 cut(s) 1037, 1179
HpyCH4III ACNGT 1 cut(s) 110
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 4 cut(s) 657, 722, 1112, 1156
HpyF10VI GCNNNNNNNGC 1 cut(s) 1174
HpyF3I CTNAG 7 cut(s) 276, 311, 333, 364, 528, 537, 1203
HpySE526I ACGT 1 cut(s) 65
Hsp92I GRCGYC 1 cut(s) 1166
Hsp92II CATG 3 cut(s) 425, 946, 1079
HspAI GCGC 1 cut(s) 1166
KasI GGCGCC 1 cut(s) 1165
Ksp22I TGATCA 1 cut(s) 745
Kzo9I GATC 9 cut(s) 202, 239, 279, 304, 314, 518, 745, 768, 886
LweI GCATC 1 cut(s) 634
MaeI CTAG 5 cut(s) 192, 386, 461, 897, 1178
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 2 cut(s) 20, 984
MalI GATC 9 cut(s) 204, 241, 281, 306, 316, 520, 747, 770, 888
MboI GATC 9 cut(s) 202, 239, 279, 304, 314, 518, 745, 768, 886
MflI RGATCY 4 cut(s) 239, 279, 314, 518
MhlI GDGCHC 1 cut(s) 1220
MluCI AATT 6 cut(s) 257, 288, 340, 629, 760, 811
Mly113I GGCGCC 1 cut(s) 1166
MlyI GAGTC 3 cut(s) 159, 922, 1345
MmeI TCCRAC 2 cut(s) 151, 193
MnlI CCTC 5 cut(s) 113, 403, 856, 1202, 1349
MseI TTAA 4 cut(s) 62, 291, 431, 1272
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 1 cut(s) 891
MvaI CCWGG 1 cut(s) 891
MwoI GCNNNNNNNGC 1 cut(s) 1174
NarI GGCGCC 1 cut(s) 1166
NdeII GATC 9 cut(s) 202, 239, 279, 304, 314, 518, 745, 768, 886
NlaIII CATG 3 cut(s) 425, 946, 1079
NlaIV GGNNCC 1 cut(s) 1167
NmeAIII GCCGAG 1 cut(s) 1173
NspI RCATGY 1 cut(s) 946
NspV TTCGAA 1 cut(s) 286
PfeI GAWTC 5 cut(s) 28, 533, 799, 847, 1183
PleI GAGTC 3 cut(s) 159, 921, 1344
PluTI GGCGCC 1 cut(s) 1169
PpsI GAGTC 3 cut(s) 159, 921, 1344
PsiI TTATAA 1 cut(s) 590
Psp124BI GAGCTC 1 cut(s) 1220
Psp1406I AACGTT 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 889
PspGI CCWGG 1 cut(s) 889
PspN4I GGNNCC 1 cut(s) 1167
PstI CTGCAG 1 cut(s) 1158
PsuI RGATCY 4 cut(s) 239, 279, 314, 518
RsaI GTAC 4 cut(s) 118, 196, 338, 395
RsaNI GTAC 4 cut(s) 117, 195, 337, 394
SacI GAGCTC 1 cut(s) 1220
SaqAI TTAA 4 cut(s) 62, 291, 431, 1272
Sau3AI GATC 9 cut(s) 202, 239, 279, 304, 314, 518, 745, 768, 886
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 3 cut(s) 159, 922, 1345
ScrFI CCNGG 1 cut(s) 891
SduI GDGCHC 1 cut(s) 1220
SfaNI GCATC 1 cut(s) 634
SfcI CTRYAG 1 cut(s) 1154
SfoI GGCGCC 1 cut(s) 1167
SfuI TTCGAA 1 cut(s) 286
SmlI CTYRAG 2 cut(s) 5, 1213
SmoI CTYRAG 2 cut(s) 5, 1213
SpeI ACTAGT 1 cut(s) 896
Sse9I AATT 6 cut(s) 257, 288, 340, 629, 760, 811
SsiI CCGC 1 cut(s) 1013
SspDI GGCGCC 1 cut(s) 1165
SspMI CTAG 5 cut(s) 192, 386, 461, 897, 1178
SstI GAGCTC 1 cut(s) 1220
StyD4I CCNGG 1 cut(s) 889
TaaI ACNGT 1 cut(s) 110
TaiI ACGT 1 cut(s) 68
TaqI TCGA 7 cut(s) 261, 286, 297, 319, 674, 767, 1354
TasI AATT 6 cut(s) 257, 288, 340, 629, 760, 811
TatI WGTACW 2 cut(s) 336, 393
TfiI GAWTC 5 cut(s) 28, 533, 799, 847, 1183
Tru1I TTAA 4 cut(s) 62, 291, 431, 1272
Tru9I TTAA 4 cut(s) 62, 291, 431, 1272
TscAI CASTG 2 cut(s) 337, 601
TspDTI ATGAA 4 cut(s) 66, 315, 839, 1258
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 2 cut(s) 337, 601
XapI RAATTY 1 cut(s) 257
XbaI TCTAGA 1 cut(s) 385
XceI RCATGY 1 cut(s) 946
XspI CTAG 5 cut(s) 192, 386, 461, 897, 1178
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.