MD07G1266600.v1.1

Predicted transmembrane protein 161AB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016225
Physical Location & Seq
Reverse (-)
33492799 .. 33494169
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1266600.v1.1.491

Sequence Viewer

Length: 1371 bp
ATGGTCCAATTCTTGATCACCTACAAGAATCTGATACTCCAAATCTTACTCTCCCTCACTCTCACCCTCCTCCTCACCTTTCTCAAAATCCCCGTCTTCTTTCTCCGCGGCCTCCACACCTACATCCACCCCGACAACGTCCAAAGCGGTGTCCGCGCCGCCATCCGCCGCCCCGATGGCCCCGATTCCGCTCCTGGGCAGCCCTCCAAATCCAACCCCGAGCTCAGGAAGCGGTCAAAGTCAAAATCCAACCCCGAGTTCGATGAAAACAATGCTCAGATCTTCCGCCTCAAGCTCGACGAGGCCCACCTCCAAACCCGCCTATATTTCAATGAGTATTCCAATGCCTTCACTTTCTCTTTCGTTGCAATTTCATGTCTTTTGCTTCAGTTGTTGTACTTGAATAACGAATCGGACGATAAAAATTCTGGGTTTTTAGTTAATGGGGTTTTTGTTCCGGTTTTGTTGGGATTTGCCGGTGTTTCTAAGTTGTTGGTTTTGCTTGGTAAAATTGCGTATGAGAAATCCGCTTCGAGGCGGTCCGAGAAGCAATTGAGTGTGATTTTGGGGGCTGTGGGGTTGGTTTTAGGCAATGTGATTTGCTTCACGTTTAGTCCTAAGTTTTTCGATTTCAATTTCGAGTCCATTGATGGGTTTGGGAAGATGTTTGTTGCTGTTTTGATGGGATGCTTTTCGGGTTTTCTATTTATTCCGGCAGTGAAGAGTGCGAGGTGTTTTTGGCTTGGAACTGATCAGATTAGGTCTGATTTGGTAATGCTGTCATGTGATTGGTTTGGCAGAGCTGTTCTGTATGTGAATCATATGATGATTGTGTTGACAGTTTTGTTGTGGATTAATCCCTTGGCTGGAATGTTCGTGAACAAGAACATCAATCGCAAGAGTGGAAGTGCAGATATGTTGTTTGGAAATATCGGATTTTCACCTTCGGATTTTGCTAAATTTAGGGTTTGGTGCTTGCTGGCTACGAGTATAGTACAGATTGTTGCTTTACGCCCGAACATACAAATGTATTTGAACGAGGCATTGCTGTCTTGGTATCAACGGTTGCATGCTAGCAGGGTTCCTGACTTGGATTTCAGTAGGGCTAAGGTGTTTTTGCACAATCACTACTTATGCCTTGTAGTTCTACAGTTCCTTGGACCGCCAGTTTTGTTGCTCCTCTTTCTTGGATTGTCTCAGATCGATGGTATCTCTCCGGAAAATTACCAATACATTTGTAGTCTAGCTCCTTGCTCTGCGTTTGTGAAAGAAGTGGCTCTCTTTATGGCTTGGTGGATCCTCTTTGTTTGGACGATTTACAGTTCAGCGACCATTTTTCTATATCGGCGCTGCATTTTGTACATGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_161AB

Protein Analysis

457

Amino Acids

51.67

Weight (kDa)

9.2

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_161AB PF10268 221 - 395 5.6e-15 Predicted transmembrane protein 161AB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52180
fragaria_vesca FvH4_7g29210
malus_domestica MD01G1198800.v1.1 MD07G1266600.v1.1
prunus_persica Prupe.2G292200_v2.0.a1
pyrus_communis pycom07g24440
rosa_chinensis RchiOBHm_Chr1g0376971
rosa_laevigata RLG00000026555
rosa_roxburghii Rroxscaffold_4G00281600
rosa_rugosa Rorug01G0400000
rosa_samantha Rh1AG417200 Rh1BG376600 Rh1CG390400 Rh1DG407300
rosa_wichuraiana Rw1G036620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 191
AccII CGCG 2 cut(s) 108, 156
AccIII TCCGGA 1 cut(s) 1216
AclWI GGATC 2 cut(s) 1291, 1304
AcsI RAATTY 2 cut(s) 424, 959
AcuI CTGAAG 1 cut(s) 371
AfaI GTAC 3 cut(s) 398, 996, 1361
AfiI CCNNNNNNNGG 5 cut(s) 195, 225, 534, 651, 866
AflIII ACRYGT 1 cut(s) 1362
AgsI TTSAA 4 cut(s) 331, 403, 634, 1036
AjnI CCWGG 1 cut(s) 193
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 4 cut(s) 223, 295, 803, 1247
AluI AGCT 4 cut(s) 223, 295, 803, 1247
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 1200
AlwI GGATC 2 cut(s) 1291, 1304
Ama87I CYCGRG 2 cut(s) 218, 254
Aor13HI TCCGGA 1 cut(s) 1216
AoxI GGCC 3 cut(s) 109, 178, 303
ApeKI GCWGC 2 cut(s) 199, 1350
ApoI RAATTY 2 cut(s) 424, 959
ArsI GACNNNNNNTTYG 2 cut(s) 78, 110
AseI ATTAAT 1 cut(s) 855
AspLEI GCGC 2 cut(s) 158, 1350
AspS9I GGNCC 5 cut(s) 4, 179, 304, 540, 1160
AsuHPI GGTGA 4 cut(s) 10, 55, 67, 933
AsuNHI GCTAGC 1 cut(s) 1073
AvaI CYCGRG 2 cut(s) 218, 254
AvaII GGWCC 3 cut(s) 4, 540, 1160
BamHI GGATCC 1 cut(s) 1296
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 211, 1337
BccI CCATC 5 cut(s) 170, 170, 644, 676, 1199
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 2 cut(s) 15, 751
BcoDI GTCTC 1 cut(s) 1200
BfaI CTAG 2 cut(s) 1074, 1244
BfmI CTRYAG 1 cut(s) 1148
BfoI RGCGCY 1 cut(s) 1351
BglI GCCNNNNNGGC 1 cut(s) 177
BglII AGATCT 1 cut(s) 279
BisI GCNGC 5 cut(s) 109, 159, 169, 200, 1351
BlsI GCNGC 5 cut(s) 110, 160, 170, 201, 1352
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 3 cut(s) 4, 540, 1160
BmeT110I CYCGRG 2 cut(s) 218, 254
BmgT120I GGNCC 5 cut(s) 4, 179, 304, 540, 1160
BmiI GGNNCC 3 cut(s) 181, 1083, 1298
BmrFI CCNGG 1 cut(s) 195
BmsI GCATC 1 cut(s) 677
BmtI GCTAGC 1 cut(s) 1077
BpiI GAAGAC 1 cut(s) 88
Bpu10I CCTNAGC 2 cut(s) 224, 1107
BpuEI CTTGAG 1 cut(s) 275
Bsa29I ATCGAT 1 cut(s) 1203
BsaJI CCNNGG 4 cut(s) 106, 194, 861, 1156
BsaWI WCCGGW 2 cut(s) 457, 1216
Bsc4I CCNNNNNNNGG 5 cut(s) 195, 225, 534, 651, 866
Bse118I RCCGGY 1 cut(s) 476
Bse1I ACTGG 1 cut(s) 1166
Bse3DI GCAATG 2 cut(s) 598, 1043
BseAI TCCGGA 1 cut(s) 1216
BseBI CCWGG 1 cut(s) 195
BseCI ATCGAT 1 cut(s) 1203
BseDI CCNNGG 4 cut(s) 106, 194, 861, 1156
BseGI GGATG 3 cut(s) 123, 162, 692
BseLI CCNNNNNNNGG 5 cut(s) 195, 225, 534, 651, 866
BseMI GCAATG 2 cut(s) 598, 1043
BseMII CTCAG 3 cut(s) 238, 290, 1211
BseNI ACTGG 1 cut(s) 1166
BseRI GAGGAG 3 cut(s) 59, 62, 1169
BseXI GCAGC 2 cut(s) 211, 1337
BsgI GTGCAG 1 cut(s) 930
Bsh1236I CGCG 2 cut(s) 108, 156
BshFI GGCC 3 cut(s) 111, 180, 305
BshVI ATCGAT 1 cut(s) 1203
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 2 cut(s) 218, 254
BsiSI CCGG 4 cut(s) 458, 477, 713, 1217
BslI CCNNNNNNNGG 5 cut(s) 195, 225, 534, 651, 866
BsmAI GTCTC 1 cut(s) 1200
BsnI GGCC 3 cut(s) 111, 180, 305
BsoBI CYCGRG 2 cut(s) 218, 254
Bsp1286I GDGCHC 1 cut(s) 225
Bsp13I TCCGGA 1 cut(s) 1216
Bsp1407I TGTACA 1 cut(s) 1359
Bsp143I GATC 5 cut(s) 15, 279, 751, 1200, 1296
BspANI GGCC 3 cut(s) 111, 180, 305
BspCNI CTCAG 3 cut(s) 237, 289, 1210
BspDI ATCGAT 1 cut(s) 1203
BspEI TCCGGA 1 cut(s) 1216
BspFNI CGCG 2 cut(s) 108, 156
BspLI GGNNCC 3 cut(s) 181, 1083, 1298
BspOI GCTAGC 1 cut(s) 1077
BspPI GGATC 2 cut(s) 1291, 1304
BsrBI CCGCTC 1 cut(s) 191
BsrDI GCAATG 2 cut(s) 598, 1043
BsrFI RCCGGY 1 cut(s) 476
BsrGI TGTACA 1 cut(s) 1359
BsrI ACTGG 1 cut(s) 1166
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 4 cut(s) 106, 194, 861, 1156
BssMI GATC 5 cut(s) 15, 279, 751, 1200, 1296
BssT1I CCWWGG 2 cut(s) 861, 1156
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 4 cut(s) 841, 1065, 1152, 1322
Bst6I CTCTTC 1 cut(s) 716
BstAUI TGTACA 1 cut(s) 1359
BstC8I GCNNGC 4 cut(s) 977, 981, 1071, 1075
BstDEI CTNAG 6 cut(s) 224, 276, 486, 618, 1107, 1197
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 3 cut(s) 123, 162, 692
BstFNI CGCG 2 cut(s) 108, 156
BstH2I RGCGCY 1 cut(s) 1351
BstHHI GCGC 2 cut(s) 158, 1350
BstKTI GATC 5 cut(s) 18, 282, 754, 1203, 1299
BstMAI GTCTC 1 cut(s) 1200
BstMBI GATC 5 cut(s) 15, 279, 751, 1200, 1296
BstMWI GCNNNNNNNGC 3 cut(s) 153, 177, 229
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 2 cut(s) 1073, 1366
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 1 cut(s) 1148
BstUI CGCG 2 cut(s) 108, 156
BstV1I GCAGC 2 cut(s) 211, 1337
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 279, 1296
BstYI RGATCY 2 cut(s) 279, 1296
Bsu15I ATCGAT 1 cut(s) 1203
BsuRI GGCC 3 cut(s) 111, 180, 305
BsuTUI ATCGAT 1 cut(s) 1203
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 3 cut(s) 123, 162, 692
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 1 cut(s) 723
Cac8I GCNNGC 4 cut(s) 977, 981, 1071, 1075
CfoI GCGC 2 cut(s) 158, 1350
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 5 cut(s) 4, 179, 304, 540, 1160
Cfr42I CCGCGG 1 cut(s) 109
ClaI ATCGAT 1 cut(s) 1203
CpoI CGGWCCG 1 cut(s) 540
Csp6I GTAC 3 cut(s) 397, 995, 1360
CspI CGGWCCG 1 cut(s) 540
CviAII CATG 4 cut(s) 375, 783, 1070, 1363
CviQI GTAC 3 cut(s) 397, 995, 1360
DdeI CTNAG 6 cut(s) 224, 276, 486, 618, 1107, 1197
DpnI GATC 5 cut(s) 17, 281, 753, 1202, 1298
DpnII GATC 5 cut(s) 15, 279, 751, 1200, 1296
Eam1104I CTCTTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 716
EciI GGCGGA 2 cut(s) 155, 275
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 861, 1156
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 3 cut(s) 4, 540, 1160
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 371
Eco88I CYCGRG 2 cut(s) 218, 254
EcoICRI GAGCTC 1 cut(s) 223
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 861, 1156
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 2 cut(s) 861, 1156
FaeI CATG 4 cut(s) 378, 786, 1073, 1366
FatI CATG 4 cut(s) 374, 782, 1069, 1362
FauI CCCGC 1 cut(s) 326
FauNDI CATATG 1 cut(s) 822
FbaI TGATCA 2 cut(s) 15, 751
Fnu4HI GCNGC 5 cut(s) 109, 159, 169, 200, 1351
FokI GGATG 3 cut(s) 110, 149, 699
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 5 cut(s) 109, 159, 169, 200, 1351
FspBI CTAG 2 cut(s) 1074, 1244
GlaI GCGC 2 cut(s) 157, 1349
GluI GCNGC 5 cut(s) 109, 159, 169, 200, 1351
HaeII RGCGCY 1 cut(s) 1351
HaeIII GGCC 3 cut(s) 111, 180, 305
HapII CCGG 4 cut(s) 458, 477, 713, 1217
HhaI GCGC 2 cut(s) 158, 1350
Hin1II CATG 4 cut(s) 378, 786, 1073, 1366
Hin6I GCGC 2 cut(s) 156, 1348
HinP1I GCGC 2 cut(s) 156, 1348
HincII GTYRAC 1 cut(s) 837
HindII GTYRAC 1 cut(s) 837
HinfI GANTC 5 cut(s) 28, 185, 410, 641, 817
HpaII CCGG 4 cut(s) 458, 477, 713, 1217
HphI GGTGA 4 cut(s) 10, 55, 67, 933
Hpy166II GTNNAC 2 cut(s) 837, 880
Hpy188I TCNGA 9 cut(s) 33, 279, 415, 544, 756, 766, 935, 949, 1200
Hpy188III TCNNGA 6 cut(s) 13, 226, 877, 1085, 1217, 1368
Hpy8I GTNNAC 2 cut(s) 837, 880
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 2 cut(s) 358, 954
HpyCH4III ACNGT 4 cut(s) 841, 1065, 1152, 1322
HpyCH4IV ACGT 2 cut(s) 138, 608
HpyCH4V TGCA 5 cut(s) 368, 911, 1069, 1120, 1353
HpyF10VI GCNNNNNNNGC 3 cut(s) 153, 177, 229
HpyF3I CTNAG 6 cut(s) 224, 276, 486, 618, 1107, 1197
HpySE526I ACGT 2 cut(s) 138, 608
Hsp92II CATG 4 cut(s) 378, 786, 1073, 1366
HspAI GCGC 2 cut(s) 156, 1348
Kpn2I TCCGGA 1 cut(s) 1216
Ksp22I TGATCA 2 cut(s) 15, 751
KspI CCGCGG 1 cut(s) 109
Kzo9I GATC 5 cut(s) 15, 279, 751, 1200, 1296
LmnI GCTCC 3 cut(s) 196, 1182, 1252
Lsp1109I GCAGC 2 cut(s) 211, 1337
LweI GCATC 1 cut(s) 677
MaeI CTAG 2 cut(s) 1074, 1244
MaeII ACGT 2 cut(s) 138, 608
MalI GATC 5 cut(s) 17, 281, 753, 1202, 1298
MbiI CCGCTC 1 cut(s) 191
MboI GATC 5 cut(s) 15, 279, 751, 1200, 1296
MboII GAAGA 4 cut(s) 88, 274, 673, 733
MfeI CAATTG 1 cut(s) 551
MflI RGATCY 2 cut(s) 279, 1296
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 8 cut(s) 8, 369, 424, 510, 551, 634, 959, 1222
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 2 cut(s) 237, 273
MroI TCCGGA 1 cut(s) 1216
MseI TTAA 2 cut(s) 441, 855
MslI CAYNNNNRTG 1 cut(s) 1025
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 4 cut(s) 458, 477, 713, 1217
MspR9I CCNGG 1 cut(s) 195
MunI CAATTG 1 cut(s) 551
MvaI CCWGG 1 cut(s) 195
MvnI CGCG 2 cut(s) 108, 156
MwoI GCNNNNNNNGC 3 cut(s) 153, 177, 229
NdeI CATATG 1 cut(s) 822
NdeII GATC 5 cut(s) 15, 279, 751, 1200, 1296
NheI GCTAGC 1 cut(s) 1073
NlaIII CATG 4 cut(s) 378, 786, 1073, 1366
NlaIV GGNNCC 3 cut(s) 181, 1083, 1298
NspI RCATGY 2 cut(s) 1073, 1366
PaeI GCATGC 1 cut(s) 1073
PciI ACATGT 1 cut(s) 1362
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 4 cut(s) 28, 185, 410, 817
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 5 cut(s) 110, 160, 170, 201, 1352
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
PscI ACATGT 1 cut(s) 1362
PshBI ATTAAT 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 3 cut(s) 181, 1083, 1298
PspPI GGNCC 5 cut(s) 4, 179, 304, 540, 1160
PsuI RGATCY 2 cut(s) 279, 1296
PsyI GACNNNGTC 1 cut(s) 137
RsaI GTAC 3 cut(s) 398, 996, 1361
RsaNI GTAC 3 cut(s) 397, 995, 1360
RseI CAYNNNNRTG 1 cut(s) 1025
Rsr2I CGGWCCG 1 cut(s) 540
RsrII CGGWCCG 1 cut(s) 540
SacI GAGCTC 1 cut(s) 225
SacII CCGCGG 1 cut(s) 109
SaqAI TTAA 2 cut(s) 441, 855
SatI GCNGC 5 cut(s) 109, 159, 169, 200, 1351
Sau3AI GATC 5 cut(s) 15, 279, 751, 1200, 1296
Sau96I GGNCC 5 cut(s) 4, 179, 304, 540, 1160
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 1 cut(s) 677
SfcI CTRYAG 1 cut(s) 1148
Sfr303I CCGCGG 1 cut(s) 109
SgrBI CCGCGG 1 cut(s) 109
SinI GGWCC 3 cut(s) 4, 540, 1160
SmiMI CAYNNNNRTG 1 cut(s) 1025
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SphI GCATGC 1 cut(s) 1073
Sse9I AATT 8 cut(s) 8, 369, 424, 510, 551, 634, 959, 1222
SspMI CTAG 2 cut(s) 1074, 1244
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 2 cut(s) 861, 1156
TaaI ACNGT 4 cut(s) 841, 1065, 1152, 1322
TaiI ACGT 2 cut(s) 141, 611
TaqI TCGA 6 cut(s) 261, 297, 533, 627, 639, 1203
TasI AATT 8 cut(s) 8, 369, 424, 510, 551, 634, 959, 1222
TatI WGTACW 3 cut(s) 396, 994, 1359
TauI GCSGC 3 cut(s) 111, 161, 171
TfiI GAWTC 4 cut(s) 28, 185, 410, 817
Tru1I TTAA 2 cut(s) 441, 855
Tru9I TTAA 2 cut(s) 441, 855
TscAI CASTG 1 cut(s) 723
TseI GCWGC 2 cut(s) 199, 1350
TspDTI ATGAA 2 cut(s) 279, 363
TspRI CASTG 1 cut(s) 723
Tth111I GACNNNGTC 1 cut(s) 137
VpaK11BI GGWCC 3 cut(s) 4, 540, 1160
VspI ATTAAT 1 cut(s) 855
XapI RAATTY 2 cut(s) 424, 959
XceI RCATGY 2 cut(s) 1073, 1366
XspI CTAG 2 cut(s) 1074, 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.