FvH4_7g29210

Predicted transmembrane protein 161AB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016225
Physical Location & Seq
Reverse (-)
21569709 .. 21571549
1841 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29210.t1

Sequence Viewer

Length: 981 bp
ATGCTGCACCAATTCTTGACCTACAAGAATCTCATACTCCACCTCTCACTCTCTCTCACCCTCACTCTCCTCCTCACCTTCCTCAAACTCCCCATCTTCTTCCTCTACAACCTCCACACCTACATCCACCCCGATCACATCCCGCCCGGCTCCGCCCGCGCCGCCATCCGCCGACCCGACTCCGATTCATCCAACCCGGAGCTCCGGAAACGGCCCAAGTCCAAATCCAAGAACACCAACAAAGCCCCGGATTTCGATGAAAACAACGCCCAGATCTTCCGCCTCAAGCTCGACGAGGCCCACCTCCAGACCCGCCTCTACTTCCCGGACTACTCCAACGCCTTCACCTTCTCCGTCGTCGCCGTCTCCTCCCTCGGACTCCAAATCTACCTAAACCCTGACTCCTCTTCTTCCTCCGATTCTGATTCTTCTTCCGATTTTCTGATCAACGCGAGTTTTGTTCCGGTGCTGCTAGGTTTCGCCGCGGTTTCGAAGCTGGTGGCGGTTTTAGGGAAAGTCTCGGCGGAGAAATCGGCGTCGAGGAGGTCGGAGAAGCAGCTGAGCGCGGTGTTCGGGGTTGTGGGACTAGTGGTGGCAAGCTTATTATCTTTTAAGTACAGTCCGTCGGTTTTGGATTTTGATTTCGAGGCGGTTGATGGGAATGGTAGGCTGTTTGTTGCGGTTTCGATGGGGTGTTTGGCCGGTTTCTTGTTCATGCCGGCGGCGAAGAGTGCCAGGGCGTTTTGGCTCGGGACTGATCAGATTAGGTCGGATTTGTCAATGCTTTCGTGTGAGTGGTTTGGGAGAATGGTGCTTTATGTGAATCATGGGATGATTGTGTTTACGGTTTTGTTGTGGATCAATCCGTTGGGTGGGATGCTTGTGAACAAGAATGGGAAGGTTCGAAATGCAGAGGGTTTGTTTGGGAATGTAGGGTTTACGGCTTCGGATTTTGAGATTGCATGCTGGGAAGGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_161AB

Protein Analysis

327

Amino Acids

35.91

Weight (kDa)

8.67

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52180
fragaria_vesca FvH4_7g29210
malus_domestica MD01G1198800.v1.1 MD07G1266600.v1.1
prunus_persica Prupe.2G292200_v2.0.a1
pyrus_communis pycom07g24440
rosa_chinensis RchiOBHm_Chr1g0376971
rosa_laevigata RLG00000026555
rosa_roxburghii Rroxscaffold_4G00281600
rosa_rugosa Rorug01G0400000
rosa_samantha Rh1AG417200 Rh1BG376600 Rh1CG390400 Rh1DG407300
rosa_wichuraiana Rw1G036620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 159, 452, 485, 566
AccIII TCCGGA 1 cut(s) 204
AclWI GGATC 1 cut(s) 866
AcoI YGGCCR 1 cut(s) 699
AcyI GRCGYC 1 cut(s) 536
AfaI GTAC 1 cut(s) 617
AfiI CCNNNNNNNGG 1 cut(s) 872
AhlI ACTAGT 1 cut(s) 586
AjnI CCWGG 1 cut(s) 734
AluBI AGCT 5 cut(s) 202, 289, 496, 559, 600
AluI AGCT 5 cut(s) 202, 289, 496, 559, 600
Alw21I GWGCWC 1 cut(s) 204
Alw26I GTCTC 2 cut(s) 370, 523
AlwI GGATC 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 749
Aor13HI TCCGGA 1 cut(s) 204
AoxI GGCC 3 cut(s) 212, 297, 699
ApeKI GCWGC 3 cut(s) 4, 469, 556
AspLEI GCGC 2 cut(s) 161, 566
AspS9I GGNCC 2 cut(s) 213, 298
AsuC2I CCSGG 4 cut(s) 147, 197, 248, 326
AsuHPI GGTGA 3 cut(s) 49, 67, 337
AsuII TTCGAA 2 cut(s) 491, 904
AvaI CYCGRG 1 cut(s) 749
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 204
BbvI GCAGC 2 cut(s) 456, 568
BccI CCATC 4 cut(s) 101, 173, 650, 682
BceAI ACGGC 3 cut(s) 227, 347, 957
BciT130I CCWGG 1 cut(s) 736
BclI TGATCA 2 cut(s) 444, 757
BcnI CCSGG 4 cut(s) 147, 197, 248, 326
BcoDI GTCTC 2 cut(s) 370, 523
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 2 cut(s) 473, 587
BglII AGATCT 1 cut(s) 273
BisI GCNGC 6 cut(s) 5, 162, 470, 483, 557, 723
BlpI GCTNAGC 1 cut(s) 560
BlsI GCNGC 6 cut(s) 6, 163, 471, 484, 558, 724
Bme1390I CCNGG 5 cut(s) 147, 197, 248, 326, 736
BmeT110I CYCGRG 1 cut(s) 749
BmgT120I GGNCC 2 cut(s) 213, 298
BmiI GGNNCC 2 cut(s) 151, 976
BmrFI CCNGG 5 cut(s) 147, 197, 248, 326, 736
BmsI GCATC 1 cut(s) 867
BpmI CTGGAG 1 cut(s) 290
Bpu1102I GCTNAGC 1 cut(s) 560
Bpu14I TTCGAA 2 cut(s) 491, 904
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 4 cut(s) 147, 197, 248, 326
BsaHI GRCGYC 1 cut(s) 536
BsaJI CCNNGG 4 cut(s) 246, 373, 483, 735
BsaWI WCCGGW 2 cut(s) 204, 463
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 1 cut(s) 872
Bse118I RCCGGY 2 cut(s) 701, 718
BseAI TCCGGA 1 cut(s) 204
BseBI CCWGG 1 cut(s) 736
BseDI CCNNGG 4 cut(s) 246, 373, 483, 735
BseGI GGATG 6 cut(s) 123, 138, 165, 188, 837, 882
BseLI CCNNNNNNNGG 1 cut(s) 872
BseMII CTCAG 1 cut(s) 551
BseRI GAGGAG 5 cut(s) 59, 62, 358, 394, 556
BseXI GCAGC 2 cut(s) 456, 568
BseYI CCCAGC 1 cut(s) 966
Bsh1236I CGCG 4 cut(s) 159, 452, 485, 566
BshFI GGCC 3 cut(s) 214, 299, 701
BsiHKAI GWGCWC 1 cut(s) 204
BsiHKCI CYCGRG 1 cut(s) 749
BsiSI CCGG 8 cut(s) 147, 197, 205, 248, 326, 464, 702, 719
BslFI GGGAC 2 cut(s) 597, 766
BslI CCNNNNNNNGG 1 cut(s) 872
BsmAI GTCTC 2 cut(s) 370, 523
BsmBI CGTCTC 1 cut(s) 370
BsmFI GGGAC 2 cut(s) 597, 766
BsnI GGCC 3 cut(s) 214, 299, 701
BsoBI CYCGRG 1 cut(s) 749
Bsp119I TTCGAA 2 cut(s) 491, 904
Bsp1286I GDGCHC 1 cut(s) 204
Bsp13I TCCGGA 1 cut(s) 204
Bsp143I GATC 5 cut(s) 133, 273, 444, 757, 858
Bsp1720I GCTNAGC 1 cut(s) 560
BspANI GGCC 3 cut(s) 214, 299, 701
BspCNI CTCAG 1 cut(s) 552
BspEI TCCGGA 1 cut(s) 204
BspFNI CGCG 4 cut(s) 159, 452, 485, 566
BspLI GGNNCC 2 cut(s) 151, 976
BspPI GGATC 1 cut(s) 866
BspT104I TTCGAA 2 cut(s) 491, 904
BsrFI RCCGGY 2 cut(s) 701, 718
BssAI RCCGGY 2 cut(s) 701, 718
BssECI CCNNGG 4 cut(s) 246, 373, 483, 735
BssMI GATC 5 cut(s) 133, 273, 444, 757, 858
BssNI GRCGYC 1 cut(s) 536
Bst2UI CCWGG 1 cut(s) 736
Bst4CI ACNGT 2 cut(s) 620, 847
Bst6I CTCTTC 2 cut(s) 412, 722
BstACI GRCGYC 1 cut(s) 536
BstBI TTCGAA 2 cut(s) 491, 904
BstC8I GCNNGC 4 cut(s) 157, 598, 720, 964
BstDEI CTNAG 1 cut(s) 560
BstDSI CCRYGG 1 cut(s) 483
BstF5I GGATG 6 cut(s) 123, 138, 165, 188, 837, 882
BstFNI CGCG 4 cut(s) 159, 452, 485, 566
BstHHI GCGC 2 cut(s) 161, 566
BstKTI GATC 5 cut(s) 136, 276, 447, 760, 861
BstMAI GTCTC 2 cut(s) 370, 523
BstMBI GATC 5 cut(s) 133, 273, 444, 757, 858
BstMWI GCNNNNNNNGC 3 cut(s) 156, 161, 731
BstNI CCWGG 1 cut(s) 736
BstNSI RCATGY 1 cut(s) 966
BstSCI CCNGG 5 cut(s) 145, 195, 246, 324, 734
BstUI CGCG 4 cut(s) 159, 452, 485, 566
BstV1I GCAGC 2 cut(s) 456, 568
BstX2I RGATCY 1 cut(s) 273
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 3 cut(s) 214, 299, 701
BtgI CCRYGG 1 cut(s) 483
BtsCI GGATG 6 cut(s) 123, 138, 165, 188, 837, 882
Cac8I GCNNGC 4 cut(s) 157, 598, 720, 964
CfoI GCGC 2 cut(s) 161, 566
Cfr10I RCCGGY 2 cut(s) 701, 718
Cfr13I GGNCC 2 cut(s) 213, 298
Cfr42I CCGCGG 1 cut(s) 486
CseI GACGC 1 cut(s) 525
Csp6I GTAC 1 cut(s) 616
CviAII CATG 3 cut(s) 715, 827, 963
CviQI GTAC 1 cut(s) 616
DdeI CTNAG 1 cut(s) 560
DpnI GATC 5 cut(s) 135, 275, 446, 759, 860
DpnII GATC 5 cut(s) 133, 273, 444, 757, 858
EaeI YGGCCR 1 cut(s) 699
Eam1104I CTCTTC 2 cut(s) 412, 722
EarI CTCTTC 2 cut(s) 412, 722
EciI GGCGGA 4 cut(s) 142, 158, 269, 539
Ecl136II GAGCTC 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 204
Eco53kI GAGCTC 1 cut(s) 202
Eco88I CYCGRG 1 cut(s) 749
EcoICRI GAGCTC 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 734
EcoT38I GRGCYC 1 cut(s) 204
Esp3I CGTCTC 1 cut(s) 370
FaeI CATG 3 cut(s) 718, 830, 966
FaiI YATR 5 cut(s) 35, 716, 819, 828, 964
FaqI GGGAC 2 cut(s) 597, 766
FatI CATG 3 cut(s) 714, 826, 962
FauI CCCGC 3 cut(s) 150, 164, 320
FbaI TGATCA 2 cut(s) 444, 757
Fnu4HI GCNGC 6 cut(s) 5, 162, 470, 483, 557, 723
FokI GGATG 6 cut(s) 110, 125, 152, 175, 844, 889
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 6 cut(s) 5, 162, 470, 483, 557, 723
FspBI CTAG 2 cut(s) 473, 587
GlaI GCGC 2 cut(s) 160, 565
GluI GCNGC 6 cut(s) 5, 162, 470, 483, 557, 723
GsaI CCCAGC 1 cut(s) 970
GsuI CTGGAG 1 cut(s) 290
HaeIII GGCC 3 cut(s) 214, 299, 701
HapII CCGG 8 cut(s) 147, 197, 205, 248, 326, 464, 702, 719
HgaI GACGC 1 cut(s) 525
HhaI GCGC 2 cut(s) 161, 566
Hin1I GRCGYC 1 cut(s) 536
Hin1II CATG 3 cut(s) 718, 830, 966
Hin6I GCGC 2 cut(s) 159, 564
HinP1I GCGC 2 cut(s) 159, 564
HindIII AAGCTT 1 cut(s) 598
HinfI GANTC 8 cut(s) 28, 179, 185, 378, 401, 419, 425, 823
HpaII CCGG 8 cut(s) 147, 197, 205, 248, 326, 464, 702, 719
HphI GGTGA 3 cut(s) 49, 67, 337
Hpy166II GTNNAC 3 cut(s) 843, 886, 939
Hpy188III TCNNGA 5 cut(s) 16, 205, 307, 751, 978
Hpy8I GTNNAC 3 cut(s) 843, 886, 939
Hpy99I CGWCG 5 cut(s) 296, 359, 362, 541, 628
HpyAV CCTTC 5 cut(s) 88, 352, 358, 892, 965
HpyCH4III ACNGT 2 cut(s) 620, 847
HpyCH4V TGCA 3 cut(s) 7, 911, 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 161, 731
HpyF3I CTNAG 1 cut(s) 560
Hsp92I GRCGYC 1 cut(s) 536
Hsp92II CATG 3 cut(s) 718, 830, 966
HspAI GCGC 2 cut(s) 159, 564
Kpn2I TCCGGA 1 cut(s) 204
KroI GCCGGC 1 cut(s) 718
KroNI GCCGGC 1 cut(s) 720
Ksp22I TGATCA 2 cut(s) 444, 757
KspI CCGCGG 1 cut(s) 486
Kzo9I GATC 5 cut(s) 133, 273, 444, 757, 858
LmnI GCTCC 3 cut(s) 155, 199, 207
Lsp1109I GCAGC 2 cut(s) 456, 568
LweI GCATC 1 cut(s) 867
MaeI CTAG 2 cut(s) 473, 587
MalI GATC 5 cut(s) 135, 275, 446, 759, 860
MboI GATC 5 cut(s) 133, 273, 444, 757, 858
MboII GAAGA 8 cut(s) 88, 91, 268, 399, 402, 420, 423, 739
MflI RGATCY 1 cut(s) 273
MhlI GDGCHC 1 cut(s) 204
MluCI AATT 1 cut(s) 11
MlyI GAGTC 3 cut(s) 173, 372, 395
MmeI TCCRAC 4 cut(s) 216, 360, 528, 750
MroI TCCGGA 1 cut(s) 204
MroNI GCCGGC 1 cut(s) 718
MseI TTAA 1 cut(s) 612
MspA1I CMGCKG 2 cut(s) 485, 559
MspI CCGG 8 cut(s) 147, 197, 205, 248, 326, 464, 702, 719
MspR9I CCNGG 5 cut(s) 147, 197, 248, 326, 736
MvaI CCWGG 1 cut(s) 736
MvnI CGCG 4 cut(s) 159, 452, 485, 566
MwoI GCNNNNNNNGC 3 cut(s) 156, 161, 731
NaeI GCCGGC 1 cut(s) 720
NciI CCSGG 4 cut(s) 147, 197, 248, 326
NdeII GATC 5 cut(s) 133, 273, 444, 757, 858
NgoMIV GCCGGC 1 cut(s) 718
NlaIII CATG 3 cut(s) 718, 830, 966
NlaIV GGNNCC 2 cut(s) 151, 976
NmeAIII GCCGAG 1 cut(s) 500
NspI RCATGY 1 cut(s) 966
NspV TTCGAA 2 cut(s) 491, 904
PaeI GCATGC 1 cut(s) 966
PdiI GCCGGC 1 cut(s) 720
PfeI GAWTC 5 cut(s) 28, 185, 419, 425, 823
PfoI TCCNGGA 1 cut(s) 324
PkrI GCNGC 6 cut(s) 6, 163, 471, 484, 558, 724
PleI GAGTC 3 cut(s) 173, 372, 395
PpsI GAGTC 3 cut(s) 173, 372, 395
Psp124BI GAGCTC 1 cut(s) 204
Psp6I CCWGG 1 cut(s) 734
PspFI CCCAGC 1 cut(s) 966
PspGI CCWGG 1 cut(s) 734
PspN4I GGNNCC 2 cut(s) 151, 976
PspPI GGNCC 2 cut(s) 213, 298
PsuI RGATCY 1 cut(s) 273
PvuII CAGCTG 1 cut(s) 559
RsaI GTAC 1 cut(s) 617
RsaNI GTAC 1 cut(s) 616
SacI GAGCTC 1 cut(s) 204
SacII CCGCGG 1 cut(s) 486
SaqAI TTAA 1 cut(s) 612
SatI GCNGC 6 cut(s) 5, 162, 470, 483, 557, 723
Sau3AI GATC 5 cut(s) 133, 273, 444, 757, 858
Sau96I GGNCC 2 cut(s) 213, 298
SchI GAGTC 3 cut(s) 173, 372, 395
ScrFI CCNGG 5 cut(s) 147, 197, 248, 326, 736
SduI GDGCHC 1 cut(s) 204
SfaNI GCATC 1 cut(s) 867
Sfr303I CCGCGG 1 cut(s) 486
SfuI TTCGAA 2 cut(s) 491, 904
SgrBI CCGCGG 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SpeI ACTAGT 1 cut(s) 586
SphI GCATGC 1 cut(s) 966
Sse9I AATT 1 cut(s) 11
SspMI CTAG 2 cut(s) 473, 587
SstI GAGCTC 1 cut(s) 204
StyD4I CCNGG 5 cut(s) 145, 195, 246, 324, 734
TaaI ACNGT 2 cut(s) 620, 847
TaqI TCGA 7 cut(s) 255, 291, 491, 539, 645, 686, 904
TasI AATT 1 cut(s) 11
TatI WGTACW 1 cut(s) 615
TauI GCSGC 3 cut(s) 164, 485, 725
TfiI GAWTC 5 cut(s) 28, 185, 419, 425, 823
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TseI GCWGC 3 cut(s) 4, 469, 556
TspDTI ATGAA 3 cut(s) 177, 273, 703
TspGWI ACGGA 3 cut(s) 343, 612, 855
XceI RCATGY 1 cut(s) 966
XspI CTAG 2 cut(s) 473, 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.