MD01G1198800.v1.1

Predicted transmembrane protein 161AB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016225
Physical Location & Seq
Reverse (-)
29637933 .. 29639303
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1198800.v1.1.491

Sequence Viewer

Length: 840 bp
ATGCTCCAATTCTTGATCACCTACAAGAATCTAATACTCCAACTCTCAGTCTCCCTCACTCTCACCCTCCTCCTCACCTTCCTCAAAATCCCCATCTTCTTCCTCCGCGGCCTCCACACCTACATCCACCCCGACAACGTCCAGAGCGGCGTCCGCGCCGCCATCCGCCGGCCCGATGGCCCCGATTCCGCTTCTGGGCAGCCCTCAAAATCTAACCCCGAGCTCAGGAAGCGGTCAAGGTCAAATCCAAACCCGAGTTCGATGAAAACAATGCTCAGATCTTCCGCCTCAAGCTCGACGAGGCCCACCTCCAAACCCACCTGTATTTCAATGATTAATGGGGTTTTTGTTCCGGTTCTGCTGGGATTTGCCGGTGTTTCTAAGCTGTTGATTTTGCATGGTAAAATTGCTTATGAGATATCCGCTTCGAGGCGTTCCGAGAAGCAACTGAGTGTGCTTTTAGGGGCTGCGGGATTGGTTTTAGGAAATGTGATTTGCTTCACGTTTAGTCCCAAGTTTATTGATTTCCATTTGGAGTCTGTTGATGGGTTTGCGAAAATGTTTATTGCTGTTTTGATGGGATGCTTTTCGGGTTTTTTGTTTATACCGGCGGTGAAGAGTGCGAGGTGTTTTTGGCTTGGGAGTCTCCTCTTGCTTGGACTTTCGCAGATTGATGGCATCTCTTCGGAAAATTACCAATACATTTGTAGTCTATCGTCGTGCTCTGCCCTTGTGAAGGAAGTGGCTCTCTTTATGGCTTGGTGGATTCTCTTTGTTTGGACAATTTACAGTTCAGCGACCATTTTTCTTTATCGGCGCTGCATTTTGTATGTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_161AB

Protein Analysis

280

Amino Acids

30.85

Weight (kDa)

9.78

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52180
fragaria_vesca FvH4_7g29210
malus_domestica MD01G1198800.v1.1 MD07G1266600.v1.1
prunus_persica Prupe.2G292200_v2.0.a1
pyrus_communis pycom07g24440
rosa_chinensis RchiOBHm_Chr1g0376971
rosa_laevigata RLG00000026555
rosa_roxburghii Rroxscaffold_4G00281600
rosa_rugosa Rorug01G0400000
rosa_samantha Rh1AG417200 Rh1BG376600 Rh1CG390400 Rh1DG407300
rosa_wichuraiana Rw1G036620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 147
AccII CGCG 2 cut(s) 108, 156
AcyI GRCGYC 1 cut(s) 150
AfiI CCNNNNNNNGG 5 cut(s) 168, 195, 225, 429, 736
AgsI TTSAA 1 cut(s) 330
AjuI GAANNNNNNNTTGG 2 cut(s) 241, 273
AluBI AGCT 3 cut(s) 223, 294, 385
AluI AGCT 3 cut(s) 223, 294, 385
Alw21I GWGCWC 2 cut(s) 225, 725
Alw26I GTCTC 2 cut(s) 55, 650
Ama87I CYCGRG 2 cut(s) 218, 253
AoxI GGCC 4 cut(s) 109, 170, 178, 302
ApeKI GCWGC 3 cut(s) 199, 467, 819
AseI ATTAAT 1 cut(s) 336
AspLEI GCGC 2 cut(s) 158, 819
AspS9I GGNCC 3 cut(s) 171, 179, 303
AsuHPI GGTGA 4 cut(s) 10, 55, 67, 625
AvaI CYCGRG 2 cut(s) 218, 253
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 225, 725
BbvI GCAGC 3 cut(s) 211, 454, 806
BccI CCATC 6 cut(s) 101, 170, 170, 539, 571, 668
BclI TGATCA 1 cut(s) 15
BcoDI GTCTC 2 cut(s) 55, 650
BfoI RGCGCY 1 cut(s) 820
BglII AGATCT 1 cut(s) 278
BisI GCNGC 6 cut(s) 109, 148, 159, 200, 468, 820
BlsI GCNGC 6 cut(s) 110, 149, 160, 201, 469, 821
BmeT110I CYCGRG 2 cut(s) 218, 253
BmgT120I GGNCC 3 cut(s) 171, 179, 303
BmiI GGNNCC 1 cut(s) 181
BmsI GCATC 2 cut(s) 572, 687
Bpu10I CCTNAGC 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 274
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 106
BsaWI WCCGGW 1 cut(s) 352
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 195, 225, 429, 736
Bse118I RCCGGY 3 cut(s) 168, 371, 607
BseDI CCNNGG 1 cut(s) 106
BseGI GGATG 3 cut(s) 123, 162, 587
BseLI CCNNNNNNNGG 5 cut(s) 168, 195, 225, 429, 736
BseMII CTCAG 4 cut(s) 60, 238, 289, 440
BseRI GAGGAG 3 cut(s) 59, 62, 638
BseXI GCAGC 3 cut(s) 211, 454, 806
BseYI CCCAGC 1 cut(s) 361
Bsh1236I CGCG 2 cut(s) 108, 156
BshFI GGCC 4 cut(s) 111, 172, 180, 304
BsiHKAI GWGCWC 2 cut(s) 225, 725
BsiHKCI CYCGRG 2 cut(s) 218, 253
BsiSI CCGG 4 cut(s) 169, 353, 372, 608
BslFI GGGAC 1 cut(s) 495
BslI CCNNNNNNNGG 5 cut(s) 168, 195, 225, 429, 736
BsmAI GTCTC 2 cut(s) 55, 650
BsmFI GGGAC 1 cut(s) 495
BsnI GGCC 4 cut(s) 111, 172, 180, 304
BsoBI CYCGRG 2 cut(s) 218, 253
Bsp1286I GDGCHC 2 cut(s) 225, 725
Bsp143I GATC 2 cut(s) 15, 278
BspANI GGCC 4 cut(s) 111, 172, 180, 304
BspCNI CTCAG 4 cut(s) 59, 237, 288, 441
BspFNI CGCG 2 cut(s) 108, 156
BspLI GGNNCC 1 cut(s) 181
BsrBI CCGCTC 1 cut(s) 147
BsrFI RCCGGY 3 cut(s) 168, 371, 607
BssAI RCCGGY 3 cut(s) 168, 371, 607
BssECI CCNNGG 1 cut(s) 106
BssMI GATC 2 cut(s) 15, 278
BssNI GRCGYC 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 791
Bst6I CTCTTC 2 cut(s) 611, 688
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 1 cut(s) 170
BstDEI CTNAG 5 cut(s) 46, 224, 275, 381, 449
BstDSI CCRYGG 1 cut(s) 106
BstENI CCTNNNNNAGG 1 cut(s) 734
BstF5I GGATG 3 cut(s) 123, 162, 587
BstFNI CGCG 2 cut(s) 108, 156
BstH2I RGCGCY 1 cut(s) 820
BstHHI GCGC 2 cut(s) 158, 819
BstKTI GATC 2 cut(s) 18, 281
BstMAI GTCTC 2 cut(s) 55, 650
BstMBI GATC 2 cut(s) 15, 278
BstMWI GCNNNNNNNGC 2 cut(s) 153, 229
BstUI CGCG 2 cut(s) 108, 156
BstV1I GCAGC 3 cut(s) 211, 454, 806
BstX2I RGATCY 1 cut(s) 278
BstYI RGATCY 1 cut(s) 278
BsuRI GGCC 4 cut(s) 111, 172, 180, 304
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 3 cut(s) 123, 162, 587
Cac8I GCNNGC 1 cut(s) 170
CfoI GCGC 2 cut(s) 158, 819
Cfr10I RCCGGY 3 cut(s) 168, 371, 607
Cfr13I GGNCC 3 cut(s) 171, 179, 303
Cfr42I CCGCGG 1 cut(s) 109
CseI GACGC 1 cut(s) 139
CviAII CATG 1 cut(s) 398
DdeI CTNAG 5 cut(s) 46, 224, 275, 381, 449
DpnI GATC 2 cut(s) 17, 280
DpnII GATC 2 cut(s) 15, 278
Eam1104I CTCTTC 2 cut(s) 611, 688
EarI CTCTTC 2 cut(s) 611, 688
EciI GGCGGA 2 cut(s) 155, 274
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 225
Eco32I GATATC 1 cut(s) 420
Eco53kI GAGCTC 1 cut(s) 223
Eco88I CYCGRG 2 cut(s) 218, 253
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 734
EcoRV GATATC 1 cut(s) 420
EcoT38I GRGCYC 1 cut(s) 225
FaeI CATG 1 cut(s) 401
FaiI YATR 5 cut(s) 399, 414, 605, 755, 831
FaqI GGGAC 1 cut(s) 495
FatI CATG 1 cut(s) 397
FauI CCCGC 1 cut(s) 463
FbaI TGATCA 1 cut(s) 15
Fnu4HI GCNGC 6 cut(s) 109, 148, 159, 200, 468, 820
FokI GGATG 3 cut(s) 110, 149, 594
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 6 cut(s) 109, 148, 159, 200, 468, 820
GlaI GCGC 2 cut(s) 157, 818
GluI GCNGC 6 cut(s) 109, 148, 159, 200, 468, 820
GsaI CCCAGC 1 cut(s) 365
HaeII RGCGCY 1 cut(s) 820
HaeIII GGCC 4 cut(s) 111, 172, 180, 304
HapII CCGG 4 cut(s) 169, 353, 372, 608
HgaI GACGC 1 cut(s) 139
HhaI GCGC 2 cut(s) 158, 819
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 1 cut(s) 401
Hin6I GCGC 2 cut(s) 156, 817
HinP1I GCGC 2 cut(s) 156, 817
HinfI GANTC 5 cut(s) 28, 185, 536, 643, 766
HpaII CCGG 4 cut(s) 169, 353, 372, 608
HphI GGTGA 4 cut(s) 10, 55, 67, 625
Hpy188I TCNGA 3 cut(s) 278, 439, 688
Hpy188III TCNNGA 4 cut(s) 13, 142, 226, 837
Hpy99I CGWCG 2 cut(s) 301, 721
HpyAV CCTTC 2 cut(s) 88, 730
HpyCH4III ACNGT 1 cut(s) 791
HpyCH4IV ACGT 2 cut(s) 138, 503
HpyCH4V TGCA 2 cut(s) 397, 822
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 229
HpyF3I CTNAG 5 cut(s) 46, 224, 275, 381, 449
HpySE526I ACGT 2 cut(s) 138, 503
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 1 cut(s) 401
HspAI GCGC 2 cut(s) 156, 817
KroI GCCGGC 1 cut(s) 168
KroNI GCCGGC 1 cut(s) 170
Ksp22I TGATCA 1 cut(s) 15
KspI CCGCGG 1 cut(s) 109
Kzo9I GATC 2 cut(s) 15, 278
LmnI GCTCC 1 cut(s) 9
LpnPI CCDG 9 cut(s) 155, 180, 182, 211, 334, 347, 366, 385, 621
Lsp1109I GCAGC 3 cut(s) 211, 454, 806
LweI GCATC 2 cut(s) 572, 687
MaeII ACGT 2 cut(s) 138, 503
MalI GATC 2 cut(s) 17, 280
MbiI CCGCTC 1 cut(s) 147
MboI GATC 2 cut(s) 15, 278
MboII GAAGA 5 cut(s) 88, 91, 273, 628, 675
MflI RGATCY 1 cut(s) 278
MhlI GDGCHC 2 cut(s) 225, 725
MluCI AATT 4 cut(s) 8, 405, 691, 783
MlyI GAGTC 2 cut(s) 545, 652
MmeI TCCRAC 1 cut(s) 64
MroNI GCCGGC 1 cut(s) 168
MseI TTAA 1 cut(s) 336
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 4 cut(s) 169, 353, 372, 608
MvnI CGCG 2 cut(s) 108, 156
MwoI GCNNNNNNNGC 2 cut(s) 153, 229
NaeI GCCGGC 1 cut(s) 170
NdeII GATC 2 cut(s) 15, 278
NgoMIV GCCGGC 1 cut(s) 168
NlaIII CATG 1 cut(s) 401
NlaIV GGNNCC 1 cut(s) 181
PdiI GCCGGC 1 cut(s) 170
PfeI GAWTC 3 cut(s) 28, 185, 766
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 6 cut(s) 110, 149, 160, 201, 469, 821
PleI GAGTC 2 cut(s) 544, 651
PpsI GAGTC 2 cut(s) 544, 651
PshBI ATTAAT 1 cut(s) 336
Psp124BI GAGCTC 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 181
PspPI GGNCC 3 cut(s) 171, 179, 303
PsuI RGATCY 1 cut(s) 278
PsyI GACNNNGTC 1 cut(s) 137
SacI GAGCTC 1 cut(s) 225
SacII CCGCGG 1 cut(s) 109
SaqAI TTAA 1 cut(s) 336
SatI GCNGC 6 cut(s) 109, 148, 159, 200, 468, 820
Sau3AI GATC 2 cut(s) 15, 278
Sau96I GGNCC 3 cut(s) 171, 179, 303
SchI GAGTC 2 cut(s) 545, 652
SduI GDGCHC 2 cut(s) 225, 725
SfaNI GCATC 2 cut(s) 572, 687
Sfr303I CCGCGG 1 cut(s) 109
SgrBI CCGCGG 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 289
SmoI CTYRAG 1 cut(s) 289
Sse9I AATT 4 cut(s) 8, 405, 691, 783
SstI GAGCTC 1 cut(s) 225
TaaI ACNGT 1 cut(s) 791
TaiI ACGT 2 cut(s) 141, 506
TaqI TCGA 3 cut(s) 260, 296, 428
TasI AATT 4 cut(s) 8, 405, 691, 783
TauI GCSGC 3 cut(s) 111, 150, 161
TfiI GAWTC 3 cut(s) 28, 185, 766
Tru1I TTAA 1 cut(s) 336
Tru9I TTAA 1 cut(s) 336
TseI GCWGC 3 cut(s) 199, 467, 819
TspDTI ATGAA 1 cut(s) 278
Tth111I GACNNNGTC 1 cut(s) 137
VspI ATTAAT 1 cut(s) 336
XagI CCTNNNNNAGG 1 cut(s) 734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.