pycom07g24440

Predicted transmembrane protein 161AB

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0016225
Physical Location & Seq
Reverse (-)
25160304 .. 25161674
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g24440.1

Sequence Viewer

Length: 1371 bp
ATGGTCCAATTCTTGATCACCTACAAGAATCTGATACTCCAAATCTCACTCTCCCTCACTCTCACCCTCCTCCTCACCTTTCTCAAAATCCCCGTCTTCTTTCTCCGCGGCCTTCACACCTACATCCACCCCGACAACGTCCAGAGCGGCGTCCGGGCCGCCATCCGCCGCCCCGACGGCCCCGATTCCGCTTCTGGGCAGCCTTCCAAATCCAACCCCGAGCTCAGGAAGCGGTCAAAGTCAAAATCCAACCCCGAGTTCGATGAAAACAATGCTCAGATCTTCCGCCTCAAGCTCGACGAGGCCCACCTCCAAACCCGCCTATATTTCAATGAGTATTCCAATGCCTTCACTTTCTCTTTCGTTGCAATTTCATGTCTTTTGCTTCAGTTGTTGTACTTGAATAACGAATCGGACGATAAAAATTCTGGGTTTTTAGTTAATGGGGTTTTTGTTCCGGTTTTGTTGGGATTTGCCGGTGTTTCTAAGCTGTTGGTTTTGCTTGGTAAAGTTGCGTATGAGAAATCCGCTTCGAGGCGGTCCGAGAAGCAATTGAGTGTGATTTTGGGGGCCGTGGGGTTGGTTTTAGGCAATGCGATTTGCTTCACGTTTAGTCCTAAGTTTTTCGATTTCAATTTCGAGTCTATTGATGGGTTCGGGAAGATGTTTATTGCTGTTTTGATGGGATGCTTTTCGGGGTTTCTATTTATTCCGGCAGTGAAGAGTGCGAGGTGTTTTTGGCTTGGAACTGATCAGATTAGGTCTGATTTGGTAATGCTGTCATGTGATTGGTTTGGCAGAGCTGTTCTGTATGTGAATCATATGATGATTGTGTTCACCGTTTTGTTGTGGATTAATCCCTTGGCTGGAATGTTCGTGAACAAGAACATCGATCGCAAGAGTGGAAGTGCGGATATGTTGTTTGGAAATATCGGATTTTCACCTTCGGATTTTGCTAAATTTAGGGTTTGGTGCTTGCTGGCTACGAGTTTAGTACAGATTGTTGCTTTACGCCCAAACATACAAATGTATTTGAACGAGGCATTGCTGTCTTGGTATCAACGGTTGCATGCTAGCAGGGTTCCTGACTTGGATTTCAGTAGGGCTAAGGTGTTTTTGCACAATCACTACTTATGCCTTGTAGTTCTACAATTCCTTGGACCACCCGTTTTGTTGCTCCTCTTTCTTGGATTGTCTCAGATCGATGGTGTCTCTCCGGAAAATTACCCATACATTTGTAGTCTAGCTCCTTGCTCTGCGTTTGTGAAAGAAGTGGCTCTCTTTATGGCTTGGTGGATCCTCTTTGTTTGGACGATTTACAGTTCAGCGACCATTTTTCTTTATCGGCGCTGCATTTTGTACATGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tmemb_161AB

Protein Analysis

457

Amino Acids

51.61

Weight (kDa)

9.14

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tmemb_161AB PF10268 221 - 395 6.8e-15 Predicted transmembrane protein 161AB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016225)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52180
fragaria_vesca FvH4_7g29210
malus_domestica MD01G1198800.v1.1 MD07G1266600.v1.1
prunus_persica Prupe.2G292200_v2.0.a1
pyrus_communis pycom07g24440
rosa_chinensis RchiOBHm_Chr1g0376971
rosa_laevigata RLG00000026555
rosa_roxburghii Rroxscaffold_4G00281600
rosa_rugosa Rorug01G0400000
rosa_samantha Rh1AG417200 Rh1BG376600 Rh1CG390400 Rh1DG407300
rosa_wichuraiana Rw1G036620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 147
AccII CGCG 1 cut(s) 108
AccIII TCCGGA 1 cut(s) 1216
AclWI GGATC 2 cut(s) 1291, 1304
AcsI RAATTY 2 cut(s) 424, 959
AcuI CTGAAG 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 3 cut(s) 398, 996, 1361
AfiI CCNNNNNNNGG 4 cut(s) 195, 225, 534, 866
AgsI TTSAA 4 cut(s) 331, 403, 634, 1036
AjuI GAANNNNNNNTTGG 2 cut(s) 242, 274
AluBI AGCT 5 cut(s) 223, 295, 490, 803, 1247
AluI AGCT 5 cut(s) 223, 295, 490, 803, 1247
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 2 cut(s) 1200, 1216
AlwI GGATC 2 cut(s) 1291, 1304
Ama87I CYCGRG 2 cut(s) 218, 254
Aor13HI TCCGGA 1 cut(s) 1216
AoxI GGCC 5 cut(s) 109, 156, 178, 303, 570
ApeKI GCWGC 2 cut(s) 199, 1350
ApoI RAATTY 2 cut(s) 424, 959
ArsI GACNNNNNNTTYG 2 cut(s) 78, 110
AseI ATTAAT 1 cut(s) 855
AspLEI GCGC 1 cut(s) 1350
AspS9I GGNCC 7 cut(s) 4, 156, 179, 304, 540, 570, 1160
AsuC2I CCSGG 1 cut(s) 155
AsuHPI GGTGA 5 cut(s) 10, 55, 67, 829, 933
AsuNHI GCTAGC 1 cut(s) 1073
AvaI CYCGRG 2 cut(s) 218, 254
AvaII GGWCC 3 cut(s) 4, 540, 1160
BamHI GGATCC 1 cut(s) 1296
BanII GRGCYC 1 cut(s) 225
BbsI GAAGAC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 225
BbvI GCAGC 2 cut(s) 211, 1337
BccI CCATC 4 cut(s) 170, 644, 676, 1199
BceAI ACGGC 2 cut(s) 193, 557
BclI TGATCA 2 cut(s) 15, 751
BcnI CCSGG 1 cut(s) 155
BcoDI GTCTC 2 cut(s) 1200, 1216
BfaI CTAG 2 cut(s) 1074, 1244
BfoI RGCGCY 1 cut(s) 1351
BglI GCCNNNNNGGC 1 cut(s) 177
BglII AGATCT 1 cut(s) 279
BisI GCNGC 6 cut(s) 109, 148, 159, 169, 200, 1351
BlsI GCNGC 6 cut(s) 110, 149, 160, 170, 201, 1352
Bme1390I CCNGG 1 cut(s) 155
Bme18I GGWCC 3 cut(s) 4, 540, 1160
BmeT110I CYCGRG 2 cut(s) 218, 254
BmgT120I GGNCC 7 cut(s) 4, 156, 179, 304, 540, 570, 1160
BmiI GGNNCC 4 cut(s) 181, 571, 1083, 1298
BmrFI CCNGG 1 cut(s) 155
BmsI GCATC 1 cut(s) 677
BmtI GCTAGC 1 cut(s) 1077
BpiI GAAGAC 1 cut(s) 88
Bpu10I CCTNAGC 2 cut(s) 224, 1107
BpuEI CTTGAG 1 cut(s) 275
BpuMI CCSGG 1 cut(s) 155
Bsa29I ATCGAT 2 cut(s) 891, 1203
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 4 cut(s) 106, 573, 861, 1156
BsaWI WCCGGW 2 cut(s) 457, 1216
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 225, 534, 866
Bse118I RCCGGY 1 cut(s) 476
Bse3DI GCAATG 2 cut(s) 598, 1043
BseAI TCCGGA 1 cut(s) 1216
BseCI ATCGAT 2 cut(s) 891, 1203
BseDI CCNNGG 4 cut(s) 106, 573, 861, 1156
BseGI GGATG 3 cut(s) 123, 162, 692
BseLI CCNNNNNNNGG 4 cut(s) 195, 225, 534, 866
BseMI GCAATG 2 cut(s) 598, 1043
BseMII CTCAG 3 cut(s) 238, 290, 1211
BseRI GAGGAG 3 cut(s) 59, 62, 1169
BseXI GCAGC 2 cut(s) 211, 1337
Bsh1236I CGCG 1 cut(s) 108
Bsh1285I CGRYCG 1 cut(s) 895
BshFI GGCC 5 cut(s) 111, 158, 180, 305, 572
BshVI ATCGAT 2 cut(s) 891, 1203
BsiEI CGRYCG 1 cut(s) 895
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 2 cut(s) 218, 254
BsiSI CCGG 5 cut(s) 154, 458, 477, 713, 1217
BslI CCNNNNNNNGG 4 cut(s) 195, 225, 534, 866
BsmAI GTCTC 2 cut(s) 1200, 1216
BsnI GGCC 5 cut(s) 111, 158, 180, 305, 572
BsoBI CYCGRG 2 cut(s) 218, 254
Bsp1286I GDGCHC 1 cut(s) 225
Bsp13I TCCGGA 1 cut(s) 1216
Bsp1407I TGTACA 1 cut(s) 1359
Bsp143I GATC 6 cut(s) 15, 279, 751, 892, 1200, 1296
BspANI GGCC 5 cut(s) 111, 158, 180, 305, 572
BspCNI CTCAG 3 cut(s) 237, 289, 1210
BspDI ATCGAT 2 cut(s) 891, 1203
BspEI TCCGGA 1 cut(s) 1216
BspFNI CGCG 1 cut(s) 108
BspLI GGNNCC 4 cut(s) 181, 571, 1083, 1298
BspOI GCTAGC 1 cut(s) 1077
BspPI GGATC 2 cut(s) 1291, 1304
BsrBI CCGCTC 1 cut(s) 147
BsrDI GCAATG 2 cut(s) 598, 1043
BsrFI RCCGGY 1 cut(s) 476
BsrGI TGTACA 1 cut(s) 1359
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 4 cut(s) 106, 573, 861, 1156
BssMI GATC 6 cut(s) 15, 279, 751, 892, 1200, 1296
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 2 cut(s) 861, 1156
Bst4CI ACNGT 3 cut(s) 841, 1065, 1322
Bst6I CTCTTC 1 cut(s) 716
BstACI GRCGYC 1 cut(s) 150
BstAUI TGTACA 1 cut(s) 1359
BstC8I GCNNGC 4 cut(s) 977, 981, 1071, 1075
BstDEI CTNAG 6 cut(s) 224, 276, 486, 618, 1107, 1197
BstDSI CCRYGG 2 cut(s) 106, 573
BstF5I GGATG 3 cut(s) 123, 162, 692
BstFNI CGCG 1 cut(s) 108
BstH2I RGCGCY 1 cut(s) 1351
BstHHI GCGC 1 cut(s) 1350
BstKTI GATC 6 cut(s) 18, 282, 754, 895, 1203, 1299
BstMAI GTCTC 2 cut(s) 1200, 1216
BstMBI GATC 6 cut(s) 15, 279, 751, 892, 1200, 1296
BstMCI CGRYCG 1 cut(s) 895
BstMWI GCNNNNNNNGC 2 cut(s) 177, 229
BstNSI RCATGY 1 cut(s) 1073
BstSCI CCNGG 1 cut(s) 153
BstUI CGCG 1 cut(s) 108
BstV1I GCAGC 2 cut(s) 211, 1337
BstV2I GAAGAC 1 cut(s) 88
BstX2I RGATCY 2 cut(s) 279, 1296
BstYI RGATCY 2 cut(s) 279, 1296
Bsu15I ATCGAT 2 cut(s) 891, 1203
BsuRI GGCC 5 cut(s) 111, 158, 180, 305, 572
BsuTUI ATCGAT 2 cut(s) 891, 1203
BtgI CCRYGG 2 cut(s) 106, 573
BtsCI GGATG 3 cut(s) 123, 162, 692
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 1 cut(s) 723
Cac8I GCNNGC 4 cut(s) 977, 981, 1071, 1075
CfoI GCGC 1 cut(s) 1350
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 7 cut(s) 4, 156, 179, 304, 540, 570, 1160
Cfr42I CCGCGG 1 cut(s) 109
ClaI ATCGAT 2 cut(s) 891, 1203
CpoI CGGWCCG 1 cut(s) 540
CseI GACGC 1 cut(s) 139
Csp6I GTAC 3 cut(s) 397, 995, 1360
CspCI CAANNNNNGTGG 2 cut(s) 1152, 1187
CspI CGGWCCG 1 cut(s) 540
CviAII CATG 4 cut(s) 375, 783, 1070, 1363
CviQI GTAC 3 cut(s) 397, 995, 1360
DdeI CTNAG 6 cut(s) 224, 276, 486, 618, 1107, 1197
DpnI GATC 6 cut(s) 17, 281, 753, 894, 1202, 1298
DpnII GATC 6 cut(s) 15, 279, 751, 892, 1200, 1296
Eam1104I CTCTTC 1 cut(s) 716
EarI CTCTTC 1 cut(s) 716
EciI GGCGGA 2 cut(s) 155, 275
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 861, 1156
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 3 cut(s) 4, 540, 1160
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 371
Eco88I CYCGRG 2 cut(s) 218, 254
EcoICRI GAGCTC 1 cut(s) 223
EcoT14I CCWWGG 2 cut(s) 861, 1156
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 2 cut(s) 861, 1156
FaeI CATG 4 cut(s) 378, 786, 1073, 1366
FatI CATG 4 cut(s) 374, 782, 1069, 1362
FauI CCCGC 1 cut(s) 326
FauNDI CATATG 1 cut(s) 822
FbaI TGATCA 2 cut(s) 15, 751
Fnu4HI GCNGC 6 cut(s) 109, 148, 159, 169, 200, 1351
FokI GGATG 3 cut(s) 110, 149, 699
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 6 cut(s) 109, 148, 159, 169, 200, 1351
FspBI CTAG 2 cut(s) 1074, 1244
GlaI GCGC 1 cut(s) 1349
GluI GCNGC 6 cut(s) 109, 148, 159, 169, 200, 1351
HaeII RGCGCY 1 cut(s) 1351
HaeIII GGCC 5 cut(s) 111, 158, 180, 305, 572
HapII CCGG 5 cut(s) 154, 458, 477, 713, 1217
HgaI GACGC 1 cut(s) 139
HhaI GCGC 1 cut(s) 1350
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 4 cut(s) 378, 786, 1073, 1366
Hin6I GCGC 1 cut(s) 1348
HinP1I GCGC 1 cut(s) 1348
HinfI GANTC 5 cut(s) 28, 185, 410, 641, 817
HpaII CCGG 5 cut(s) 154, 458, 477, 713, 1217
HphI GGTGA 5 cut(s) 10, 55, 67, 829, 933
Hpy166II GTNNAC 2 cut(s) 837, 880
Hpy188I TCNGA 9 cut(s) 33, 279, 415, 544, 756, 766, 935, 949, 1200
Hpy188III TCNNGA 7 cut(s) 13, 142, 226, 658, 877, 1085, 1217
Hpy8I GTNNAC 2 cut(s) 837, 880
Hpy99I CGWCG 2 cut(s) 179, 302
HpyAV CCTTC 4 cut(s) 122, 213, 358, 954
HpyCH4III ACNGT 3 cut(s) 841, 1065, 1322
HpyCH4IV ACGT 2 cut(s) 138, 608
HpyCH4V TGCA 4 cut(s) 368, 1069, 1120, 1353
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 229
HpyF3I CTNAG 6 cut(s) 224, 276, 486, 618, 1107, 1197
HpySE526I ACGT 2 cut(s) 138, 608
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 4 cut(s) 378, 786, 1073, 1366
HspAI GCGC 1 cut(s) 1348
Kpn2I TCCGGA 1 cut(s) 1216
Ksp22I TGATCA 2 cut(s) 15, 751
KspI CCGCGG 1 cut(s) 109
Kzo9I GATC 6 cut(s) 15, 279, 751, 892, 1200, 1296
LmnI GCTCC 2 cut(s) 1182, 1252
Lsp1109I GCAGC 2 cut(s) 211, 1337
LweI GCATC 1 cut(s) 677
MaeI CTAG 2 cut(s) 1074, 1244
MaeII ACGT 2 cut(s) 138, 608
MalI GATC 6 cut(s) 17, 281, 753, 894, 1202, 1298
MbiI CCGCTC 1 cut(s) 147
MboI GATC 6 cut(s) 15, 279, 751, 892, 1200, 1296
MboII GAAGA 4 cut(s) 88, 274, 673, 733
MfeI CAATTG 1 cut(s) 551
MflI RGATCY 2 cut(s) 279, 1296
MhlI GDGCHC 1 cut(s) 225
MluCI AATT 8 cut(s) 8, 369, 424, 551, 634, 959, 1151, 1222
MlyI GAGTC 1 cut(s) 650
MmeI TCCRAC 2 cut(s) 237, 273
MroI TCCGGA 1 cut(s) 1216
MseI TTAA 2 cut(s) 441, 855
MslI CAYNNNNRTG 1 cut(s) 1025
MspA1I CMGCKG 1 cut(s) 108
MspI CCGG 5 cut(s) 154, 458, 477, 713, 1217
MspR9I CCNGG 1 cut(s) 155
MunI CAATTG 1 cut(s) 551
MvnI CGCG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 177, 229
NciI CCSGG 1 cut(s) 155
NdeI CATATG 1 cut(s) 822
NdeII GATC 6 cut(s) 15, 279, 751, 892, 1200, 1296
NheI GCTAGC 1 cut(s) 1073
NlaIII CATG 4 cut(s) 378, 786, 1073, 1366
NlaIV GGNNCC 4 cut(s) 181, 571, 1083, 1298
NspI RCATGY 1 cut(s) 1073
PaeI GCATGC 1 cut(s) 1073
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 4 cut(s) 28, 185, 410, 817
PflFI GACNNNGTC 1 cut(s) 137
PkrI GCNGC 6 cut(s) 110, 149, 160, 170, 201, 1352
Ple19I CGATCG 1 cut(s) 895
PleI GAGTC 1 cut(s) 649
PpsI GAGTC 1 cut(s) 649
PshBI ATTAAT 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 225
PspN4I GGNNCC 4 cut(s) 181, 571, 1083, 1298
PspPI GGNCC 7 cut(s) 4, 156, 179, 304, 540, 570, 1160
PsuI RGATCY 2 cut(s) 279, 1296
PsyI GACNNNGTC 1 cut(s) 137
PvuI CGATCG 1 cut(s) 895
RsaI GTAC 3 cut(s) 398, 996, 1361
RsaNI GTAC 3 cut(s) 397, 995, 1360
RseI CAYNNNNRTG 1 cut(s) 1025
Rsr2I CGGWCCG 1 cut(s) 540
RsrII CGGWCCG 1 cut(s) 540
SacI GAGCTC 1 cut(s) 225
SacII CCGCGG 1 cut(s) 109
SaqAI TTAA 2 cut(s) 441, 855
SatI GCNGC 6 cut(s) 109, 148, 159, 169, 200, 1351
Sau3AI GATC 6 cut(s) 15, 279, 751, 892, 1200, 1296
Sau96I GGNCC 7 cut(s) 4, 156, 179, 304, 540, 570, 1160
SchI GAGTC 1 cut(s) 650
ScrFI CCNGG 1 cut(s) 155
SduI GDGCHC 1 cut(s) 225
SfaNI GCATC 1 cut(s) 677
Sfr303I CCGCGG 1 cut(s) 109
SgrBI CCGCGG 1 cut(s) 109
SinI GGWCC 3 cut(s) 4, 540, 1160
SmiMI CAYNNNNRTG 1 cut(s) 1025
SmlI CTYRAG 1 cut(s) 290
SmoI CTYRAG 1 cut(s) 290
SphI GCATGC 1 cut(s) 1073
Sse9I AATT 8 cut(s) 8, 369, 424, 551, 634, 959, 1151, 1222
SspMI CTAG 2 cut(s) 1074, 1244
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 153
StyI CCWWGG 2 cut(s) 861, 1156
TaaI ACNGT 3 cut(s) 841, 1065, 1322
TaiI ACGT 2 cut(s) 141, 611
TaqI TCGA 7 cut(s) 261, 297, 533, 627, 639, 891, 1203
TasI AATT 8 cut(s) 8, 369, 424, 551, 634, 959, 1151, 1222
TatI WGTACW 3 cut(s) 396, 994, 1359
TauI GCSGC 4 cut(s) 111, 150, 161, 171
TfiI GAWTC 4 cut(s) 28, 185, 410, 817
Tru1I TTAA 2 cut(s) 441, 855
Tru9I TTAA 2 cut(s) 441, 855
TscAI CASTG 1 cut(s) 723
TseI GCWGC 2 cut(s) 199, 1350
TspDTI ATGAA 2 cut(s) 279, 363
TspRI CASTG 1 cut(s) 723
Tth111I GACNNNGTC 1 cut(s) 137
VpaK11BI GGWCC 3 cut(s) 4, 540, 1160
VspI ATTAAT 1 cut(s) 855
XapI RAATTY 2 cut(s) 424, 959
XceI RCATGY 1 cut(s) 1073
XspI CTAG 2 cut(s) 1074, 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.