AT5G54300

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012843
Physical Location & Seq
Reverse (-)
22053969 .. 22055443
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54300.1

Sequence Viewer

Length: 981 bp
ATGCCTCTTCTTCTCCCTACCTCTTCCTTCAAAACGACGGCCACCGTCGTAATCGCCGGCGTTTCATCTATTGCCACGGCGATGATTCTCACGGTCCCTTCCGTTTCCCATTTCGTAGTCTCTTGCTTTCCGATTATTTACGATAACACCGTCTTCCTCTTAAAACCACCGTATCTTTACTTAGTCATCAACTCTATCATCGTCTGCATCATCGCTACTTCTAAACTCACTCATAAATCGTCCTCCGTCGATGACTCTGAGATCTCGGAAGTAGTGACTCCAATACCGATACCGGTACCGGTTCATTTACCTTCTGATATCGATTCCGGTTATTTAAACGTGGTTCATGTGGTTTCTGATTATACCGGATTTGTTGAGAAAATCGATGACGTGTCGATTAATCCAACGGTGGAGGCTATCCGGAAGTTTCCAGAAGTTCAGGAGGCGGAGAAGTCGAAAGAGAGTAGCGATTCGCCGGAACCGGAGACGGAGAAGCCAAAATTGAAGAATGATTCGCCGGAGATTTCGATTCTGAAGCACAGTACGAGAAAACCGCCGCGATTTAATCAGCAAAAATCTCTTAAATCTAATTCAGAAGGTGGAAATAAAAAGACGGCGTTGGGAGTGACGAAGCCACCGAGGAGACAAGACACGCTGGAGACGACGTGGAAGAAGATAACGGAAGGACGTTCAACGCCGTTAACTAAGCACTTGACTAAATCCGACACGTGGCAAGAAAGAGCTCACGTGCAAAGCTCACCAGAAAATAAGGAGAAGATGACCAAGTCCGAGAATTTAAAGGACATAAATACCCCCACGGAGGAGAAGACGGTGTTGAAACGCGAGCCGTCGCCGGGACAGGAGGAGTTGAATCGGCGCGTTGAAGCGTTTATAAAGAAGTTTAACGAAGAGATGAGATTGCAAAGATTAGAATCTTTGGCTAAATACAATGAAATGGTAAATGGAGGGACTCGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

326

Amino Acids

36.45

Weight (kDa)

8.31

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 44 - 76 1.2e-10 Domain of unknown function (DUF4408)
DUF761 PF05553 287 - 321 1.5e-16 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54300
fragaria_vesca FvH4_3g20920
malus_domestica MD03G1219300.v1.1 MD11G1237400.v1.1
prunus_persica Prupe.4G189800_v2.0.a1
pyrus_communis pycom03g16840 pycom11g20870
rosa_chinensis RchiOBHm_Chr5g0035351
rosa_laevigata RLG00000033619
rosa_multiflora Rmu_sc0007948.1_g000015 Rmu_sc0032746.1_g000001
rosa_roxburghii Rroxscaffold_1G00045050
rosa_rugosa Rorug05G0151400
rosa_samantha Rh5AG244500 Rh5BG244000 Rh5CG275800 Rh5DG253000
rosa_wichuraiana Rw5G022330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 893
Acc65I GGTACC 1 cut(s) 295
AccB1I GGYRCC 1 cut(s) 295
AccII CGCG 3 cut(s) 559, 843, 879
AccIII TCCGGA 1 cut(s) 420
AciI CCGC 3 cut(s) 446, 554, 557
AcoI YGGCCR 1 cut(s) 39
AcsI RAATTY 1 cut(s) 793
AcuI CTGAAG 1 cut(s) 554
AcvI CACGTG 2 cut(s) 729, 748
AfaI GTAC 2 cut(s) 297, 544
AfiI CCNNNNNNNGG 3 cut(s) 729, 820, 854
AflIII ACRYGT 2 cut(s) 390, 726
AgeI ACCGGT 2 cut(s) 292, 298
AgsI TTSAA 6 cut(s) 31, 505, 693, 838, 871, 884
AjiI CACGTC 2 cut(s) 391, 666
AluBI AGCT 2 cut(s) 743, 756
AluI AGCT 2 cut(s) 743, 756
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 4 cut(s) 124, 479, 637, 653
Aor13HI TCCGGA 1 cut(s) 420
AoxI GGCC 1 cut(s) 39
ApoI RAATTY 1 cut(s) 793
AseI ATTAAT 1 cut(s) 399
AsiGI ACCGGT 2 cut(s) 292, 298
Asp718I GGTACC 1 cut(s) 295
AspLEI GCGC 1 cut(s) 879
AspS9I GGNCC 1 cut(s) 94
AsuC2I CCSGG 1 cut(s) 855
AsuHPI GGTGA 1 cut(s) 750
AvaII GGWCC 1 cut(s) 94
BaeI ACNNNNGTAYC 2 cut(s) 155, 188
BanI GGYRCC 1 cut(s) 295
BanII GRGCYC 1 cut(s) 745
BbrPI CACGTG 2 cut(s) 729, 748
BbsI GAAGAC 2 cut(s) 145, 833
Bbv12I GWGCWC 1 cut(s) 745
BceAI ACGGC 5 cut(s) 54, 93, 630, 682, 832
BcnI CCSGG 1 cut(s) 855
BcoDI GTCTC 4 cut(s) 124, 479, 637, 653
BglII AGATCT 1 cut(s) 261
BisI GCNGC 1 cut(s) 557
BlsI GCNGC 1 cut(s) 558
Bme1390I CCNGG 1 cut(s) 855
Bme18I GGWCC 1 cut(s) 94
BmgBI CACGTC 2 cut(s) 391, 666
BmgT120I GGNCC 1 cut(s) 94
BmiI GGNNCC 3 cut(s) 96, 297, 480
BmrFI CCNGG 1 cut(s) 855
BmsI GCATC 1 cut(s) 216
BpiI GAAGAC 2 cut(s) 145, 833
BpmI CTGGAG 1 cut(s) 677
BpuMI CCSGG 1 cut(s) 855
Bsa29I ATCGAT 2 cut(s) 321, 384
BsaAI YACGTR 2 cut(s) 729, 748
BsaBI GATNNNNATC 1 cut(s) 931
BsaJI CCNNGG 3 cut(s) 75, 638, 816
BsaWI WCCGGW 6 cut(s) 292, 298, 326, 365, 420, 481
BsaXI ACNNNNNCTCC 2 cut(s) 650, 680
Bsc4I CCNNNNNNNGG 3 cut(s) 729, 820, 854
Bse118I RCCGGY 3 cut(s) 56, 292, 298
Bse8I GATNNNNATC 1 cut(s) 931
BseAI TCCGGA 1 cut(s) 420
BseCI ATCGAT 2 cut(s) 321, 384
BseDI CCNNGG 3 cut(s) 75, 638, 816
BseJI GATNNNNATC 1 cut(s) 931
BseLI CCNNNNNNNGG 3 cut(s) 729, 820, 854
BseMII CTCAG 1 cut(s) 249
BseRI GAGGAG 3 cut(s) 655, 836, 878
Bsh1236I CGCG 3 cut(s) 559, 843, 879
BshFI GGCC 1 cut(s) 41
BshNI GGYRCC 1 cut(s) 295
BshTI ACCGGT 2 cut(s) 292, 298
BshVI ATCGAT 2 cut(s) 321, 384
BsiHKAI GWGCWC 1 cut(s) 745
BslFI GGGAC 2 cut(s) 80, 870
BslI CCNNNNNNNGG 3 cut(s) 729, 820, 854
BsmAI GTCTC 4 cut(s) 124, 479, 637, 653
BsmBI CGTCTC 2 cut(s) 479, 653
BsmFI GGGAC 2 cut(s) 80, 870
BsnI GGCC 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 745
Bsp13I TCCGGA 1 cut(s) 420
Bsp143I GATC 1 cut(s) 261
BspACI CCGC 3 cut(s) 446, 554, 557
BspANI GGCC 1 cut(s) 41
BspCNI CTCAG 1 cut(s) 250
BspDI ATCGAT 2 cut(s) 321, 384
BspEI TCCGGA 1 cut(s) 420
BspFNI CGCG 3 cut(s) 559, 843, 879
BspLI GGNNCC 3 cut(s) 96, 297, 480
BspT107I GGYRCC 1 cut(s) 295
BsrFI RCCGGY 3 cut(s) 56, 292, 298
BssAI RCCGGY 3 cut(s) 56, 292, 298
BssECI CCNNGG 3 cut(s) 75, 638, 816
BssMI GATC 1 cut(s) 261
Bst4CI ACNGT 7 cut(s) 46, 94, 151, 171, 409, 542, 832
Bst6I CTCTTC 3 cut(s) 12, 28, 903
BstBAI YACGTR 2 cut(s) 729, 748
BstC8I GCNNGC 2 cut(s) 58, 845
BstDEI CTNAG 3 cut(s) 181, 258, 705
BstDSI CCRYGG 2 cut(s) 75, 816
BstFNI CGCG 3 cut(s) 559, 843, 879
BstHHI GCGC 1 cut(s) 879
BstKTI GATC 1 cut(s) 264
BstMAI GTCTC 4 cut(s) 124, 479, 637, 653
BstMBI GATC 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 853
BstUI CGCG 3 cut(s) 559, 843, 879
BstV2I GAAGAC 2 cut(s) 145, 833
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
Bsu15I ATCGAT 2 cut(s) 321, 384
BsuRI GGCC 1 cut(s) 41
BsuTUI ATCGAT 2 cut(s) 321, 384
BtgI CCRYGG 2 cut(s) 75, 816
BtgZI GCGATG 2 cut(s) 95, 196
BtrI CACGTC 2 cut(s) 391, 666
Cac8I GCNNGC 2 cut(s) 58, 845
CfoI GCGC 1 cut(s) 879
Cfr10I RCCGGY 3 cut(s) 56, 292, 298
Cfr13I GGNCC 1 cut(s) 94
ClaI ATCGAT 2 cut(s) 321, 384
Csp6I GTAC 2 cut(s) 296, 543
CspAI ACCGGT 2 cut(s) 292, 298
CviAII CATG 1 cut(s) 347
CviJI RGCY 8 cut(s) 41, 416, 496, 634, 743, 756, 847, 941
CviKI_1 RGCY 8 cut(s) 41, 416, 496, 634, 743, 756, 847, 941
CviQI GTAC 2 cut(s) 296, 543
DdeI CTNAG 3 cut(s) 181, 258, 705
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
DraI TTTAAA 2 cut(s) 336, 798
EaeI YGGCCR 1 cut(s) 39
Eam1104I CTCTTC 3 cut(s) 12, 28, 903
EarI CTCTTC 3 cut(s) 12, 28, 903
EciI GGCGGA 1 cut(s) 461
Ecl136II GAGCTC 1 cut(s) 743
Eco24I GRGCYC 1 cut(s) 745
Eco32I GATATC 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 94
Eco53kI GAGCTC 1 cut(s) 743
Eco57I CTGAAG 1 cut(s) 554
Eco72I CACGTG 2 cut(s) 729, 748
EcoICRI GAGCTC 1 cut(s) 743
EcoRV GATATC 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 745
Esp3I CGTCTC 2 cut(s) 479, 653
FaeI CATG 1 cut(s) 350
FaiI YATR 5 cut(s) 234, 348, 363, 806, 893
FaqI GGGAC 2 cut(s) 80, 870
FatI CATG 1 cut(s) 346
Fnu4HI GCNGC 1 cut(s) 557
FriOI GRGCYC 1 cut(s) 745
Fsp4HI GCNGC 1 cut(s) 557
GlaI GCGC 1 cut(s) 878
GluI GCNGC 1 cut(s) 557
GsuI CTGGAG 1 cut(s) 677
HaeIII GGCC 1 cut(s) 41
HhaI GCGC 1 cut(s) 879
Hin1II CATG 1 cut(s) 350
Hin6I GCGC 1 cut(s) 877
HinP1I GCGC 1 cut(s) 877
HincII GTYRAC 1 cut(s) 702
HindII GTYRAC 1 cut(s) 702
HpaI GTTAAC 1 cut(s) 702
HphI GGTGA 1 cut(s) 750
Hpy166II GTNNAC 1 cut(s) 702
Hpy188I TCNGA 9 cut(s) 132, 259, 268, 316, 358, 534, 595, 724, 790
Hpy188III TCNNGA 3 cut(s) 421, 431, 440
Hpy8I GTNNAC 1 cut(s) 702
Hpy99I CGWCG 5 cut(s) 40, 50, 251, 667, 853
HpyAV CCTTC 5 cut(s) 37, 108, 321, 590, 677
HpyCH4III ACNGT 7 cut(s) 46, 94, 151, 171, 409, 542, 832
HpyCH4IV ACGT 6 cut(s) 339, 390, 665, 688, 728, 747
HpyCH4V TGCA 3 cut(s) 207, 751, 922
HpyF3I CTNAG 3 cut(s) 181, 258, 705
HpySE526I ACGT 6 cut(s) 339, 390, 665, 688, 728, 747
Hsp92II CATG 1 cut(s) 350
HspAI GCGC 1 cut(s) 877
Kpn2I TCCGGA 1 cut(s) 420
KpnI GGTACC 1 cut(s) 299
KroI GCCGGC 1 cut(s) 56
KroNI GCCGGC 1 cut(s) 58
KspAI GTTAAC 1 cut(s) 702
Kzo9I GATC 1 cut(s) 261
LweI GCATC 1 cut(s) 216
MaeII ACGT 6 cut(s) 339, 390, 665, 688, 728, 747
MaeIII GTNAC 2 cut(s) 274, 625
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 8 cut(s) 15, 145, 517, 682, 685, 787, 838, 920
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 1 cut(s) 745
MluCI AATT 3 cut(s) 500, 589, 793
MlyI GAGTC 3 cut(s) 248, 271, 964
MmeI TCCRAC 2 cut(s) 428, 747
MreI CGCCGGCG 1 cut(s) 56
MroI TCCGGA 1 cut(s) 420
MroNI GCCGGC 1 cut(s) 56
MseI TTAA 8 cut(s) 161, 335, 399, 564, 582, 701, 797, 903
MslI CAYNNNNRTG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 855
MvnI CGCG 3 cut(s) 559, 843, 879
NaeI GCCGGC 1 cut(s) 58
NciI CCSGG 1 cut(s) 855
NdeII GATC 1 cut(s) 261
NgoMIV GCCGGC 1 cut(s) 56
NlaIII CATG 1 cut(s) 350
NlaIV GGNNCC 3 cut(s) 96, 297, 480
NmuCI GTSAC 2 cut(s) 274, 625
PcsI WCGNNNNNNNCGW 3 cut(s) 147, 635, 659
PdiI GCCGGC 1 cut(s) 58
PfeI GAWTC 7 cut(s) 85, 323, 470, 512, 529, 871, 932
PflFI GACNNNGTC 1 cut(s) 784
PinAI ACCGGT 2 cut(s) 292, 298
PkrI GCNGC 1 cut(s) 558
PleI GAGTC 3 cut(s) 248, 271, 964
PmaCI CACGTG 2 cut(s) 729, 748
PmlI CACGTG 2 cut(s) 729, 748
PpsI GAGTC 3 cut(s) 248, 271, 964
Ppu21I YACGTR 2 cut(s) 729, 748
PshBI ATTAAT 1 cut(s) 399
PsiI TTATAA 1 cut(s) 893
Psp124BI GAGCTC 1 cut(s) 745
PspCI CACGTG 2 cut(s) 729, 748
PspN4I GGNNCC 3 cut(s) 96, 297, 480
PspPI GGNCC 1 cut(s) 94
PsuI RGATCY 1 cut(s) 261
PsyI GACNNNGTC 1 cut(s) 784
RsaI GTAC 2 cut(s) 297, 544
RsaNI GTAC 2 cut(s) 296, 543
RseI CAYNNNNRTG 1 cut(s) 80
SacI GAGCTC 1 cut(s) 745
SaqAI TTAA 8 cut(s) 161, 335, 399, 564, 582, 701, 797, 903
SatI GCNGC 1 cut(s) 557
Sau3AI GATC 1 cut(s) 261
Sau96I GGNCC 1 cut(s) 94
SchI GAGTC 3 cut(s) 248, 271, 964
ScrFI CCNGG 1 cut(s) 855
SduI GDGCHC 1 cut(s) 745
SfaNI GCATC 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 56
SinI GGWCC 1 cut(s) 94
SmiMI CAYNNNNRTG 1 cut(s) 80
Sse9I AATT 3 cut(s) 500, 589, 793
SsiI CCGC 3 cut(s) 446, 554, 557
SstI GAGCTC 1 cut(s) 745
StyD4I CCNGG 1 cut(s) 853
TaaI ACNGT 7 cut(s) 46, 94, 151, 171, 409, 542, 832
TaiI ACGT 6 cut(s) 342, 393, 668, 691, 731, 750
TaqI TCGA 6 cut(s) 249, 321, 384, 395, 455, 527
TasI AATT 3 cut(s) 500, 589, 793
TauI GCSGC 1 cut(s) 559
TfiI GAWTC 7 cut(s) 85, 323, 470, 512, 529, 871, 932
Tru1I TTAA 8 cut(s) 161, 335, 399, 564, 582, 701, 797, 903
Tru9I TTAA 8 cut(s) 161, 335, 399, 564, 582, 701, 797, 903
TseFI GTSAC 2 cut(s) 274, 625
Tsp45I GTSAC 2 cut(s) 274, 625
TspDTI ATGAA 4 cut(s) 54, 293, 335, 966
TspGWI ACGGA 5 cut(s) 91, 235, 503, 695, 833
Tth111I GACNNNGTC 1 cut(s) 784
VpaK11BI GGWCC 1 cut(s) 94
VspI ATTAAT 1 cut(s) 399
XapI RAATTY 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.