MD11G1237400.v1.1

Domain of unknown function (DUF4408)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0012843
Physical Location & Seq
Reverse (-)
34507959 .. 34509527
1569 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1237400.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGTCTACGAGCACGAGCAACACCTGGTTACTCACTCTAAAGGTTGCGCTGATATCCACTGGTGTGGTCTCCATGGCCGTGGGGCTGAAACTCTCAGCTCCAGTGGTCGCCGACGCCGTCACCTCCCATGTCCCTTCTCTCTGGAGCTCCGCCGTCTCCTGGCTCAGTCCTCTGTATCTCTACATAATCATCAACTGCATCATCATCAGCATCATCGCCTCCTCCAAGCTCCACCCCAAACCCGAAGATTTGACGCCGGTGACGGAGCCGGAGCTGGTGAGGGCCTCTCCCGTGACGACAGCAAAGATATCTGCACAAATTAGTCCCGACTACTCAGCGTACGACGGCTTCGTTCCGAGTGAGTCCGGTTACGATGAGAATGTTCTGACGAAGGTTTCGGATTCGTACGGAGGAGTGGTAGTGGAACCAATGGTTTCGGAGGTGGTGAAGGGGAAGGAGGAGAATAGCGAAGATCTGGGGGCGATTGAAGGCGGAGATGAGGCAGCCGCGGTATCGACGCCTGTAAAGACTAGTTTACAGAGGAAGGACTCTATGGAGTTCTGGTTGAACGAGAACCAGAAACCGCCGGTTTCCGCTCGTATCGGTCACAGAAAATCCGTTAAAGCTAGCCCTGAAGGGGGAAAGTCGTTGGGAGTGTCGAAGCCGAAGCGGAACGACACGCTGGAGAGCACGTGGAGGACGATAACGGAAGGGCGCTCGGTGCCGTTAACGAGGCACCTGAAGAAGTCGGACACGTGGGACACGCAAGTGCGGGGGATAGACGAGAACACGCCGCCTCCGAAGCCCAAGATGAACAAGTCGGAGACGTTCCAGGAGCGGAGCAGCGCGAACTCTCCGCATCCGAAGCCGAAAATGAACAAGTCGGAGACGTTGCAGGAGAGAAGCAGCGCGAACTCGCCGTCGCTGGCTCGGTCGTCGGGGTCGGGGCGGCTGAGGAAGGAGCCGTCGCTGAGTCAAGACGAATTGAACCGGCGGGTGGAGGCGTTCATCAACAAGTTTAACGAGGAAATGAGGCTGCAGAGGCAGGAGTCGTTGAATCAGTACCAGAAGATGGTGAACCGCGGGGCCAAGGCTCCAATGGCTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4408 DUF761

Protein Analysis

370

Amino Acids

40.38

Weight (kDa)

9.04

Isoelectric Point (pI)

54.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4408 PF14364 45 - 77 1.9e-12 Domain of unknown function (DUF4408)
DUF761 PF05553 326 - 361 3.9e-16 Cotton fibre expressed protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012843)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54300
fragaria_vesca FvH4_3g20920
malus_domestica MD03G1219300.v1.1 MD11G1237400.v1.1
prunus_persica Prupe.4G189800_v2.0.a1
pyrus_communis pycom03g16840 pycom11g20870
rosa_chinensis RchiOBHm_Chr5g0035351
rosa_laevigata RLG00000033619
rosa_multiflora Rmu_sc0007948.1_g000015 Rmu_sc0032746.1_g000001
rosa_roxburghii Rroxscaffold_1G00045050
rosa_rugosa Rorug05G0151400
rosa_samantha Rh5AG244500 Rh5BG244000 Rh5CG275800 Rh5DG253000
rosa_wichuraiana Rw5G022330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 721, 735
AccB7I CCANNNNNTGG 1 cut(s) 1072
AccBSI CCGCTC 2 cut(s) 596, 838
AccI GTMKAC 1 cut(s) 5
AccII CGCG 4 cut(s) 509, 848, 911, 1083
AcoI YGGCCR 1 cut(s) 75
AcuI CTGAAG 2 cut(s) 654, 761
AcvI CACGTG 2 cut(s) 693, 756
AcyI GRCGYC 3 cut(s) 114, 254, 518
AfaI GTAC 3 cut(s) 341, 407, 1064
AfiI CCNNNNNNNGG 5 cut(s) 159, 637, 638, 997, 1072
AflIII ACRYGT 1 cut(s) 753
AgsI TTSAA 4 cut(s) 488, 568, 988, 1057
AhlI ACTAGT 1 cut(s) 530
AjnI CCWGG 3 cut(s) 23, 158, 831
AleI CACNNNNGTG 2 cut(s) 62, 767
AluBI AGCT 5 cut(s) 98, 147, 229, 274, 626
AluI AGCT 5 cut(s) 98, 147, 229, 274, 626
Alw21I GWGCWC 3 cut(s) 14, 149, 692
Alw26I GTCTC 4 cut(s) 73, 160, 818, 881
AoxI GGCC 3 cut(s) 75, 282, 1086
ApeKI GCWGC 4 cut(s) 503, 843, 906, 1036
ArsI GACNNNNNNTTYG 4 cut(s) 379, 411, 905, 937
AspLEI GCGC 4 cut(s) 49, 717, 848, 911
AspS9I GGNCC 2 cut(s) 282, 1086
AsuHPI GGTGA 5 cut(s) 112, 271, 289, 457, 1087
AsuNHI GCTAGC 1 cut(s) 626
BanI GGYRCC 2 cut(s) 721, 735
BanII GRGCYC 1 cut(s) 149
BarI GAAGNNNNNNTAC 2 cut(s) 332, 364
BauI CACGAG 1 cut(s) 13
BbrPI CACGTG 2 cut(s) 693, 756
Bbv12I GWGCWC 3 cut(s) 14, 149, 692
BbvCI CCTCAGC 1 cut(s) 953
BbvI GCAGC 4 cut(s) 515, 855, 918, 1023
BccI CCATC 1 cut(s) 1066
BceAI ACGGC 7 cut(s) 62, 101, 137, 361, 709, 904, 949
BciT130I CCWGG 3 cut(s) 25, 160, 833
BcoDI GTCTC 4 cut(s) 73, 160, 818, 881
BcuI ACTAGT 1 cut(s) 530
BfaI CTAG 2 cut(s) 531, 627
BfmI CTRYAG 1 cut(s) 1037
BfoI RGCGCY 1 cut(s) 718
BglII AGATCT 1 cut(s) 472
BisI GCNGC 7 cut(s) 504, 507, 794, 844, 907, 950, 1037
BlsI GCNGC 7 cut(s) 505, 508, 795, 845, 908, 951, 1038
Bme1390I CCNGG 3 cut(s) 25, 160, 833
BmgT120I GGNCC 2 cut(s) 282, 1086
BmiI GGNNCC 7 cut(s) 267, 426, 723, 737, 963, 1087, 1095
BmrFI CCNGG 3 cut(s) 25, 160, 833
BmsI GCATC 3 cut(s) 207, 219, 868
BmtI GCTAGC 1 cut(s) 630
BplI GAGNNNNNCTC 2 cut(s) 271, 303
BpmI CTGGAG 3 cut(s) 84, 163, 704
Bpu10I CCTNAGC 1 cut(s) 953
BsaAI YACGTR 2 cut(s) 693, 756
BsaHI GRCGYC 3 cut(s) 114, 254, 518
BsaI GGTCTC 1 cut(s) 73
BsaJI CCNNGG 5 cut(s) 72, 78, 507, 1081, 1089
BsaWI WCCGGW 1 cut(s) 365
Bsc4I CCNNNNNNNGG 5 cut(s) 159, 637, 638, 997, 1072
Bse118I RCCGGY 3 cut(s) 256, 586, 990
Bse1I ACTGG 2 cut(s) 64, 101
BseBI CCWGG 3 cut(s) 25, 160, 833
BseDI CCNNGG 5 cut(s) 72, 78, 507, 1081, 1089
BseGI GGATG 1 cut(s) 859
BseLI CCNNNNNNNGG 5 cut(s) 159, 637, 638, 997, 1072
BseMII CTCAG 5 cut(s) 108, 178, 348, 944, 962
BseNI ACTGG 2 cut(s) 64, 101
BseRI GAGGAG 3 cut(s) 211, 426, 473
BseXI GCAGC 4 cut(s) 515, 855, 918, 1023
BsgI GTGCAG 1 cut(s) 297
Bsh1236I CGCG 4 cut(s) 509, 848, 911, 1083
Bsh1285I CGRYCG 1 cut(s) 935
BshFI GGCC 3 cut(s) 77, 284, 1088
BshNI GGYRCC 2 cut(s) 721, 735
BsiEI CGRYCG 1 cut(s) 935
BsiHKAI GWGCWC 3 cut(s) 14, 149, 692
BsiSI CCGG 5 cut(s) 257, 269, 366, 587, 991
BsiWI CGTACG 2 cut(s) 339, 405
BslFI GGGAC 3 cut(s) 116, 309, 773
BslI CCNNNNNNNGG 5 cut(s) 159, 637, 638, 997, 1072
BsmAI GTCTC 4 cut(s) 73, 160, 818, 881
BsmBI CGTCTC 3 cut(s) 160, 818, 881
BsmFI GGGAC 3 cut(s) 116, 309, 773
BsnI GGCC 3 cut(s) 77, 284, 1088
Bso31I GGTCTC 1 cut(s) 73
Bsp1286I GDGCHC 3 cut(s) 14, 149, 692
Bsp143I GATC 1 cut(s) 472
Bsp19I CCATGG 1 cut(s) 72
BspANI GGCC 3 cut(s) 77, 284, 1088
BspCNI CTCAG 5 cut(s) 107, 177, 347, 945, 963
BspFNI CGCG 4 cut(s) 509, 848, 911, 1083
BspLI GGNNCC 7 cut(s) 267, 426, 723, 737, 963, 1087, 1095
BspMAI CTGCAG 1 cut(s) 1041
BspOI GCTAGC 1 cut(s) 630
BspT107I GGYRCC 2 cut(s) 721, 735
BspTNI GGTCTC 1 cut(s) 73
BsrBI CCGCTC 2 cut(s) 596, 838
BsrFI RCCGGY 3 cut(s) 256, 586, 990
BsrI ACTGG 2 cut(s) 64, 101
BssAI RCCGGY 3 cut(s) 256, 586, 990
BssECI CCNNGG 5 cut(s) 72, 78, 507, 1081, 1089
BssMI GATC 1 cut(s) 472
BssNI GRCGYC 3 cut(s) 114, 254, 518
BssSI CACGAG 1 cut(s) 13
BssT1I CCWWGG 2 cut(s) 72, 1089
Bst2BI CACGAG 1 cut(s) 13
Bst2UI CCWGG 3 cut(s) 25, 160, 833
BstACI GRCGYC 3 cut(s) 114, 254, 518
BstBAI YACGTR 2 cut(s) 693, 756
BstC8I GCNNGC 2 cut(s) 628, 927
BstDEI CTNAG 5 cut(s) 94, 164, 334, 953, 971
BstDSI CCRYGG 4 cut(s) 72, 78, 507, 1081
BstF5I GGATG 1 cut(s) 859
BstFNI CGCG 4 cut(s) 509, 848, 911, 1083
BstH2I RGCGCY 1 cut(s) 718
BstHHI GCGC 4 cut(s) 49, 717, 848, 911
BstKTI GATC 1 cut(s) 475
BstMAI GTCTC 4 cut(s) 73, 160, 818, 881
BstMBI GATC 1 cut(s) 472
BstMCI CGRYCG 1 cut(s) 935
BstMWI GCNNNNNNNGC 5 cut(s) 721, 802, 865, 1042, 1100
BstNI CCWGG 3 cut(s) 25, 160, 833
BstSCI CCNGG 3 cut(s) 23, 158, 831
BstSFI CTRYAG 1 cut(s) 1037
BstUI CGCG 4 cut(s) 509, 848, 911, 1083
BstV1I GCAGC 4 cut(s) 515, 855, 918, 1023
BstX2I RGATCY 1 cut(s) 472
BstXI CCANNNNNNTGG 2 cut(s) 64, 79
BstYI RGATCY 1 cut(s) 472
BsuRI GGCC 3 cut(s) 77, 284, 1088
BtgI CCRYGG 4 cut(s) 72, 78, 507, 1081
BtgZI GCGATG 1 cut(s) 199
BtsCI GGATG 1 cut(s) 859
BtsIMutI CAGTG 2 cut(s) 57, 108
Cac8I GCNNGC 2 cut(s) 628, 927
CfoI GCGC 4 cut(s) 49, 717, 848, 911
Cfr10I RCCGGY 3 cut(s) 256, 586, 990
Cfr13I GGNCC 2 cut(s) 282, 1086
Cfr42I CCGCGG 2 cut(s) 510, 1084
CseI GACGC 3 cut(s) 122, 262, 526
CsiI ACCWGGT 1 cut(s) 23
Csp6I GTAC 3 cut(s) 340, 406, 1063
CviAII CATG 2 cut(s) 73, 128
CviQI GTAC 3 cut(s) 340, 406, 1063
DdeI CTNAG 5 cut(s) 94, 164, 334, 953, 971
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
EaeI YGGCCR 1 cut(s) 75
EciI GGCGGA 2 cut(s) 139, 507
Ecl136II GAGCTC 1 cut(s) 147
Eco130I CCWWGG 2 cut(s) 72, 1089
Eco24I GRGCYC 1 cut(s) 149
Eco31I GGTCTC 1 cut(s) 73
Eco32I GATATC 2 cut(s) 54, 309
Eco53kI GAGCTC 1 cut(s) 147
Eco57I CTGAAG 2 cut(s) 654, 761
Eco72I CACGTG 2 cut(s) 693, 756
EcoICRI GAGCTC 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 282
EcoRII CCWGG 3 cut(s) 23, 158, 831
EcoRV GATATC 2 cut(s) 54, 309
EcoT14I CCWWGG 2 cut(s) 72, 1089
EcoT38I GRGCYC 1 cut(s) 149
ErhI CCWWGG 2 cut(s) 72, 1089
Esp3I CGTCTC 3 cut(s) 160, 818, 881
FaeI CATG 2 cut(s) 76, 131
FaiI YATR 4 cut(s) 74, 129, 185, 554
FaqI GGGAC 3 cut(s) 116, 309, 773
FatI CATG 2 cut(s) 72, 127
FauI CCCGC 3 cut(s) 765, 987, 1076
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 7 cut(s) 504, 507, 794, 844, 907, 950, 1037
FokI GGATG 1 cut(s) 846
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 7 cut(s) 504, 507, 794, 844, 907, 950, 1037
FspBI CTAG 2 cut(s) 531, 627
GlaI GCGC 4 cut(s) 48, 716, 847, 910
GluI GCNGC 7 cut(s) 504, 507, 794, 844, 907, 950, 1037
GsuI CTGGAG 3 cut(s) 84, 163, 704
HaeII RGCGCY 1 cut(s) 718
HaeIII GGCC 3 cut(s) 77, 284, 1088
HapII CCGG 5 cut(s) 257, 269, 366, 587, 991
HgaI GACGC 3 cut(s) 122, 262, 526
HhaI GCGC 4 cut(s) 49, 717, 848, 911
Hin1I GRCGYC 3 cut(s) 114, 254, 518
Hin1II CATG 2 cut(s) 76, 131
Hin6I GCGC 4 cut(s) 47, 715, 846, 909
HinP1I GCGC 4 cut(s) 47, 715, 846, 909
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HinfI GANTC 6 cut(s) 362, 401, 548, 973, 1049, 1057
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 5 cut(s) 257, 269, 366, 587, 991
HphI GGTGA 5 cut(s) 112, 271, 289, 457, 1087
Hpy166II GTNNAC 4 cut(s) 6, 536, 729, 1078
Hpy188I TCNGA 9 cut(s) 357, 387, 400, 439, 751, 801, 823, 864, 886
Hpy188III TCNNGA 3 cut(s) 142, 326, 977
Hpy8I GTNNAC 4 cut(s) 6, 536, 729, 1078
Hpy99I CGWCG 6 cut(s) 116, 347, 520, 925, 940, 970
HpyAV CCTTC 9 cut(s) 144, 385, 442, 448, 482, 538, 629, 704, 952
HpyCH4IV ACGT 4 cut(s) 692, 755, 827, 890
HpyCH4V TGCA 4 cut(s) 198, 314, 895, 1039
HpyF10VI GCNNNNNNNGC 5 cut(s) 721, 802, 865, 1042, 1100
HpyF3I CTNAG 5 cut(s) 94, 164, 334, 953, 971
HpySE526I ACGT 4 cut(s) 692, 755, 827, 890
Hsp92I GRCGYC 3 cut(s) 114, 254, 518
Hsp92II CATG 2 cut(s) 76, 131
HspAI GCGC 4 cut(s) 47, 715, 846, 909
KspAI GTTAAC 1 cut(s) 729
KspI CCGCGG 2 cut(s) 510, 1084
Kzo9I GATC 1 cut(s) 472
Lsp1109I GCAGC 4 cut(s) 515, 855, 918, 1023
LweI GCATC 3 cut(s) 207, 219, 868
MabI ACCWGGT 1 cut(s) 23
MaeI CTAG 2 cut(s) 531, 627
MaeII ACGT 4 cut(s) 692, 755, 827, 890
MaeIII GTNAC 6 cut(s) 27, 118, 259, 292, 368, 605
MalI GATC 1 cut(s) 474
MbiI CCGCTC 2 cut(s) 596, 838
MboI GATC 1 cut(s) 472
MboII GAAGA 4 cut(s) 257, 482, 754, 1081
MflI RGATCY 1 cut(s) 472
MhlI GDGCHC 3 cut(s) 14, 149, 692
MluCI AATT 2 cut(s) 318, 983
MlyI GAGTC 4 cut(s) 371, 542, 982, 1058
MmeI TCCRAC 3 cut(s) 729, 801, 864
MseI TTAA 3 cut(s) 621, 728, 1020
MslI CAYNNNNRTG 3 cut(s) 62, 77, 767
MspA1I CMGCKG 2 cut(s) 509, 1083
MspI CCGG 5 cut(s) 257, 269, 366, 587, 991
MspR9I CCNGG 3 cut(s) 25, 160, 833
MvaI CCWGG 3 cut(s) 25, 160, 833
MvnI CGCG 4 cut(s) 509, 848, 911, 1083
MwoI GCNNNNNNNGC 5 cut(s) 721, 802, 865, 1042, 1100
NcoI CCATGG 1 cut(s) 72
NdeII GATC 1 cut(s) 472
NheI GCTAGC 1 cut(s) 626
NlaIII CATG 2 cut(s) 76, 131
NlaIV GGNNCC 7 cut(s) 267, 426, 723, 737, 963, 1087, 1095
NmuCI GTSAC 4 cut(s) 118, 259, 292, 605
OliI CACNNNNGTG 2 cut(s) 62, 767
PcsI WCGNNNNNNNCGW 4 cut(s) 114, 348, 698, 797
PfeI GAWTC 2 cut(s) 401, 1057
Pfl23II CGTACG 2 cut(s) 339, 405
PflFI GACNNNGTC 1 cut(s) 116
PflMI CCANNNNNTGG 1 cut(s) 1072
PfoI TCCNGGA 1 cut(s) 831
PkrI GCNGC 7 cut(s) 505, 508, 795, 845, 908, 951, 1038
PleI GAGTC 4 cut(s) 370, 542, 981, 1057
PmaCI CACGTG 2 cut(s) 693, 756
PmlI CACGTG 2 cut(s) 693, 756
PpsI GAGTC 4 cut(s) 370, 542, 981, 1057
Ppu21I YACGTR 2 cut(s) 693, 756
Psp124BI GAGCTC 1 cut(s) 149
Psp6I CCWGG 3 cut(s) 23, 158, 831
PspCI CACGTG 2 cut(s) 693, 756
PspGI CCWGG 3 cut(s) 23, 158, 831
PspLI CGTACG 2 cut(s) 339, 405
PspN4I GGNNCC 7 cut(s) 267, 426, 723, 737, 963, 1087, 1095
PspPI GGNCC 2 cut(s) 282, 1086
PstI CTGCAG 1 cut(s) 1041
PsuI RGATCY 1 cut(s) 472
PsyI GACNNNGTC 1 cut(s) 116
RsaI GTAC 3 cut(s) 341, 407, 1064
RsaNI GTAC 3 cut(s) 340, 406, 1063
RseI CAYNNNNRTG 3 cut(s) 62, 77, 767
SacI GAGCTC 1 cut(s) 149
SacII CCGCGG 2 cut(s) 510, 1084
SaqAI TTAA 3 cut(s) 621, 728, 1020
SatI GCNGC 7 cut(s) 504, 507, 794, 844, 907, 950, 1037
Sau3AI GATC 1 cut(s) 472
Sau96I GGNCC 2 cut(s) 282, 1086
SchI GAGTC 4 cut(s) 371, 542, 982, 1058
ScrFI CCNGG 3 cut(s) 25, 160, 833
SduI GDGCHC 3 cut(s) 14, 149, 692
SexAI ACCWGGT 1 cut(s) 23
SfaNI GCATC 3 cut(s) 207, 219, 868
SfcI CTRYAG 1 cut(s) 1037
Sfr303I CCGCGG 2 cut(s) 510, 1084
SgrAI CRCCGGYG 1 cut(s) 256
SgrBI CCGCGG 2 cut(s) 510, 1084
SmiMI CAYNNNNRTG 3 cut(s) 62, 77, 767
SpeI ACTAGT 1 cut(s) 530
Sse9I AATT 2 cut(s) 318, 983
SspMI CTAG 2 cut(s) 531, 627
SstI GAGCTC 1 cut(s) 149
StyD4I CCNGG 3 cut(s) 23, 158, 831
StyI CCWWGG 2 cut(s) 72, 1089
TaiI ACGT 4 cut(s) 695, 758, 830, 893
TaqI TCGA 2 cut(s) 515, 659
TaqII GACCGA 2 cut(s) 593, 921
TasI AATT 2 cut(s) 318, 983
TauI GCSGC 3 cut(s) 509, 796, 952
TfiI GAWTC 2 cut(s) 401, 1057
Tru1I TTAA 3 cut(s) 621, 728, 1020
Tru9I TTAA 3 cut(s) 621, 728, 1020
TscAI CASTG 2 cut(s) 64, 108
TseFI GTSAC 4 cut(s) 118, 259, 292, 605
TseI GCWGC 4 cut(s) 503, 843, 906, 1036
Tsp45I GTSAC 4 cut(s) 118, 259, 292, 605
TspDTI ATGAA 3 cut(s) 827, 890, 997
TspGWI ACGGA 4 cut(s) 278, 423, 607, 722
TspRI CASTG 2 cut(s) 64, 108
Tth111I GACNNNGTC 1 cut(s) 116
Van91I CCANNNNNTGG 1 cut(s) 1072
XcmI CCANNNNNNNNNTGG 1 cut(s) 1096
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 531, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.