Domain of unknown function (DUF4408)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G54300 |
| fragaria_vesca | FvH4_3g20920 |
| malus_domestica | MD03G1219300.v1.1 MD11G1237400.v1.1 |
| prunus_persica | Prupe.4G189800_v2.0.a1 |
| pyrus_communis | pycom03g16840 pycom11g20870 |
| rosa_chinensis | RchiOBHm_Chr5g0035351 |
| rosa_laevigata | RLG00000033619 |
| rosa_multiflora | Rmu_sc0007948.1_g000015 Rmu_sc0032746.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00045050 |
| rosa_rugosa | Rorug05G0151400 |
| rosa_samantha | Rh5AG244500 Rh5BG244000 Rh5CG275800 Rh5DG253000 |
| rosa_wichuraiana | Rw5G022330 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 727, 1042 |
| AccBSI | CCGCTC | 1 cut(s) | 769 |
| AccII | CGCG | 3 cut(s) | 110, 332, 467 |
| AclWI | GGATC | 1 cut(s) | 316 |
| AcoI | YGGCCR | 4 cut(s) | 75, 105, 718, 1024 |
| AcuI | CTGAAG | 2 cut(s) | 107, 660 |
| AcvI | CACGTG | 2 cut(s) | 699, 762 |
| AcyI | GRCGYC | 4 cut(s) | 63, 366, 513, 1043 |
| AdeI | CACNNNGTG | 1 cut(s) | 545 |
| AfaI | GTAC | 4 cut(s) | 350, 383, 425, 1010 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 331, 644, 762, 943 |
| AflIII | ACRYGT | 1 cut(s) | 759 |
| AgeI | ACCGGT | 1 cut(s) | 601 |
| AgsI | TTSAA | 5 cut(s) | 463, 583, 748, 934, 1003 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 721 |
| AjnI | CCWGG | 3 cut(s) | 530, 1011, 1035 |
| AluBI | AGCT | 5 cut(s) | 91, 147, 229, 632, 1052 |
| AluI | AGCT | 5 cut(s) | 91, 147, 229, 632, 1052 |
| Alw21I | GWGCWC | 2 cut(s) | 149, 698 |
| Alw26I | GTCTC | 3 cut(s) | 73, 440, 815 |
| AlwI | GGATC | 1 cut(s) | 316 |
| Ama87I | CYCGRG | 1 cut(s) | 878 |
| AoxI | GGCC | 9 cut(s) | 75, 105, 341, 533, 596, 718, 892, 1024, 1032 |
| ApaI | GGGCCC | 1 cut(s) | 1036 |
| ApeKI | GCWGC | 2 cut(s) | 434, 982 |
| AsiGI | ACCGGT | 1 cut(s) | 601 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 270 |
| AspLEI | GCGC | 1 cut(s) | 1045 |
| AspS9I | GGNCC | 5 cut(s) | 112, 778, 892, 1032, 1033 |
| AsuC2I | CCSGG | 3 cut(s) | 856, 879, 880 |
| AsuHPI | GGTGA | 2 cut(s) | 518, 877 |
| AsuNHI | GCTAGC | 1 cut(s) | 632 |
| AvaI | CYCGRG | 1 cut(s) | 878 |
| AvaII | GGWCC | 2 cut(s) | 112, 778 |
| BaeGI | GKGCMC | 1 cut(s) | 1036 |
| BanI | GGYRCC | 2 cut(s) | 727, 1042 |
| BanII | GRGCYC | 2 cut(s) | 149, 1036 |
| BauI | CACGAG | 1 cut(s) | 830 |
| BbrPI | CACGTG | 2 cut(s) | 699, 762 |
| BbsI | GAAGAC | 2 cut(s) | 255, 710 |
| Bbv12I | GWGCWC | 2 cut(s) | 149, 698 |
| BbvCI | CCTCAGC | 1 cut(s) | 899 |
| BbvI | GCAGC | 2 cut(s) | 446, 969 |
| BceAI | ACGGC | 8 cut(s) | 62, 281, 379, 506, 715, 733, 1011, 1030 |
| BciT130I | CCWGG | 3 cut(s) | 532, 1013, 1037 |
| BcnI | CCSGG | 3 cut(s) | 856, 879, 880 |
| BcoDI | GTCTC | 3 cut(s) | 73, 440, 815 |
| BfaI | CTAG | 2 cut(s) | 612, 633 |
| BfmI | CTRYAG | 1 cut(s) | 983 |
| BfoI | RGCGCY | 1 cut(s) | 1046 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 676, 1040 |
| Bme1390I | CCNGG | 6 cut(s) | 532, 856, 879, 880, 1013, 1037 |
| Bme18I | GGWCC | 2 cut(s) | 112, 778 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 721 |
| BmeT110I | CYCGRG | 1 cut(s) | 878 |
| BmgT120I | GGNCC | 5 cut(s) | 112, 778, 892, 1032, 1033 |
| BmiI | GGNNCC | 8 cut(s) | 99, 471, 729, 893, 909, 1033, 1034, 1044 |
| BmrFI | CCNGG | 6 cut(s) | 532, 856, 879, 880, 1013, 1037 |
| BmsI | GCATC | 4 cut(s) | 207, 219, 421, 536 |
| BmtI | GCTAGC | 1 cut(s) | 636 |
| BpiI | GAAGAC | 2 cut(s) | 255, 710 |
| BpmI | CTGGAG | 1 cut(s) | 710 |
| Bpu10I | CCTNAGC | 1 cut(s) | 899 |
| BpuEI | CTTGAG | 1 cut(s) | 23 |
| BpuMI | CCSGG | 3 cut(s) | 856, 879, 880 |
| BsaAI | YACGTR | 2 cut(s) | 699, 762 |
| BsaHI | GRCGYC | 4 cut(s) | 63, 366, 513, 1043 |
| BsaI | GGTCTC | 1 cut(s) | 440 |
| BsaJI | CCNNGG | 7 cut(s) | 72, 108, 330, 567, 878, 1027, 1035 |
| BsaWI | WCCGGW | 3 cut(s) | 100, 304, 601 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 563, 593, 683, 713 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 331, 644, 762, 943 |
| Bse118I | RCCGGY | 6 cut(s) | 59, 601, 720, 862, 894, 936 |
| Bse1I | ACTGG | 1 cut(s) | 29 |
| BseBI | CCWGG | 3 cut(s) | 532, 1013, 1037 |
| BseDI | CCNNGG | 7 cut(s) | 72, 108, 330, 567, 878, 1027, 1035 |
| BseGI | GGATG | 3 cut(s) | 183, 353, 527 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 331, 644, 762, 943 |
| BseMII | CTCAG | 4 cut(s) | 336, 365, 890, 908 |
| BseNI | ACTGG | 1 cut(s) | 29 |
| BseRI | GAGGAG | 2 cut(s) | 211, 986 |
| BseSI | GKGCMC | 1 cut(s) | 1036 |
| BseX3I | CGGCCG | 2 cut(s) | 718, 1024 |
| BseXI | GCAGC | 2 cut(s) | 446, 969 |
| BsgI | GTGCAG | 1 cut(s) | 522 |
| Bsh1236I | CGCG | 3 cut(s) | 110, 332, 467 |
| Bsh1285I | CGRYCG | 4 cut(s) | 721, 725, 855, 1027 |
| BshFI | GGCC | 9 cut(s) | 77, 107, 343, 535, 598, 720, 894, 1026, 1034 |
| BshNI | GGYRCC | 2 cut(s) | 727, 1042 |
| BshTI | ACCGGT | 1 cut(s) | 601 |
| BsiEI | CGRYCG | 4 cut(s) | 721, 725, 855, 1027 |
| BsiHKAI | GWGCWC | 2 cut(s) | 149, 698 |
| BsiHKCI | CYCGRG | 1 cut(s) | 878 |
| BsiWI | CGTACG | 1 cut(s) | 423 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 331, 644, 762, 943 |
| BsmAI | GTCTC | 3 cut(s) | 73, 440, 815 |
| BsmBI | CGTCTC | 2 cut(s) | 73, 815 |
| BsnI | GGCC | 9 cut(s) | 77, 107, 343, 535, 598, 720, 894, 1026, 1034 |
| Bso31I | GGTCTC | 1 cut(s) | 440 |
| BsoBI | CYCGRG | 1 cut(s) | 878 |
| Bsp120I | GGGCCC | 1 cut(s) | 1032 |
| Bsp1286I | GDGCHC | 3 cut(s) | 149, 698, 1036 |
| Bsp143I | GATC | 4 cut(s) | 42, 51, 321, 1020 |
| Bsp19I | CCATGG | 2 cut(s) | 72, 567 |
| BspANI | GGCC | 9 cut(s) | 77, 107, 343, 535, 598, 720, 894, 1026, 1034 |
| BspCNI | CTCAG | 4 cut(s) | 337, 366, 891, 909 |
| BspFNI | CGCG | 3 cut(s) | 110, 332, 467 |
| BspLI | GGNNCC | 8 cut(s) | 99, 471, 729, 893, 909, 1033, 1034, 1044 |
| BspMAI | CTGCAG | 1 cut(s) | 987 |
| BspOI | GCTAGC | 1 cut(s) | 636 |
| BspPI | GGATC | 1 cut(s) | 316 |
| BspQI | GCTCTTC | 1 cut(s) | 479 |
| BspT107I | GGYRCC | 2 cut(s) | 727, 1042 |
| BspTNI | GGTCTC | 1 cut(s) | 440 |
| BsrBI | CCGCTC | 1 cut(s) | 769 |
| BsrFI | RCCGGY | 6 cut(s) | 59, 601, 720, 862, 894, 936 |
| BsrI | ACTGG | 1 cut(s) | 29 |
| BssAI | RCCGGY | 6 cut(s) | 59, 601, 720, 862, 894, 936 |
| BssECI | CCNNGG | 7 cut(s) | 72, 108, 330, 567, 878, 1027, 1035 |
| BssMI | GATC | 4 cut(s) | 42, 51, 321, 1020 |
| BssNI | GRCGYC | 4 cut(s) | 63, 366, 513, 1043 |
| BssSI | CACGAG | 1 cut(s) | 830 |
| BssT1I | CCWWGG | 2 cut(s) | 72, 567 |
| Bst2BI | CACGAG | 1 cut(s) | 830 |
| Bst2UI | CCWGG | 3 cut(s) | 532, 1013, 1037 |
| Bst4CI | ACNGT | 5 cut(s) | 316, 386, 451, 912, 1008 |
| Bst6I | CTCTTC | 3 cut(s) | 2, 479, 668 |
| BstACI | GRCGYC | 4 cut(s) | 63, 366, 513, 1043 |
| BstBAI | YACGTR | 2 cut(s) | 699, 762 |
| BstC8I | GCNNGC | 4 cut(s) | 537, 551, 634, 896 |
| BstDEI | CTNAG | 4 cut(s) | 345, 374, 899, 917 |
| BstDSI | CCRYGG | 5 cut(s) | 72, 108, 330, 567, 1027 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 642 |
| BstF5I | GGATG | 3 cut(s) | 183, 353, 527 |
| BstFNI | CGCG | 3 cut(s) | 110, 332, 467 |
| BstH2I | RGCGCY | 1 cut(s) | 1046 |
| BstHHI | GCGC | 1 cut(s) | 1045 |
| BstKTI | GATC | 4 cut(s) | 45, 54, 324, 1023 |
| BstMAI | GTCTC | 3 cut(s) | 73, 440, 815 |
| BstMBI | GATC | 4 cut(s) | 42, 51, 321, 1020 |
| BstMCI | CGRYCG | 4 cut(s) | 721, 725, 855, 1027 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 160, 524, 676, 685, 988, 1040, 1049 |
| BstNI | CCWGG | 3 cut(s) | 532, 1013, 1037 |
| BstSCI | CCNGG | 6 cut(s) | 530, 854, 877, 878, 1011, 1035 |
| BstSFI | CTRYAG | 1 cut(s) | 983 |
| BstSLI | GKGCMC | 1 cut(s) | 1036 |
| BstUI | CGCG | 3 cut(s) | 110, 332, 467 |
| BstV1I | GCAGC | 2 cut(s) | 446, 969 |
| BstV2I | GAAGAC | 2 cut(s) | 255, 710 |
| BstX2I | RGATCY | 1 cut(s) | 321 |
| BstYI | RGATCY | 1 cut(s) | 321 |
| BstZI | CGGCCG | 2 cut(s) | 718, 1024 |
| BsuRI | GGCC | 9 cut(s) | 77, 107, 343, 535, 598, 720, 894, 1026, 1034 |
| BtgI | CCRYGG | 5 cut(s) | 72, 108, 330, 567, 1027 |
| BtsCI | GGATG | 3 cut(s) | 183, 353, 527 |
| BtsIMutI | CAGTG | 1 cut(s) | 83 |
| Cac8I | GCNNGC | 4 cut(s) | 537, 551, 634, 896 |
| CfoI | GCGC | 1 cut(s) | 1045 |
| Cfr10I | RCCGGY | 6 cut(s) | 59, 601, 720, 862, 894, 936 |
| Cfr13I | GGNCC | 5 cut(s) | 112, 778, 892, 1032, 1033 |
| Cfr42I | CCGCGG | 2 cut(s) | 111, 333 |
| Cfr9I | CCCGGG | 1 cut(s) | 878 |
| CseI | GACGC | 3 cut(s) | 52, 355, 521 |
| Csp6I | GTAC | 4 cut(s) | 349, 382, 424, 1009 |
| CspAI | ACCGGT | 1 cut(s) | 601 |
| CviAII | CATG | 2 cut(s) | 73, 568 |
| CviQI | GTAC | 4 cut(s) | 349, 382, 424, 1009 |
| DdeI | CTNAG | 4 cut(s) | 345, 374, 899, 917 |
| DinI | GGCGCC | 1 cut(s) | 1044 |
| DpnI | GATC | 4 cut(s) | 44, 53, 323, 1022 |
| DpnII | GATC | 4 cut(s) | 42, 51, 321, 1020 |
| DraIII | CACNNNGTG | 1 cut(s) | 545 |
| DriI | GACNNNNNGTC | 1 cut(s) | 721 |
| EaeI | YGGCCR | 4 cut(s) | 75, 105, 718, 1024 |
| EagI | CGGCCG | 2 cut(s) | 718, 1024 |
| Eam1104I | CTCTTC | 3 cut(s) | 2, 479, 668 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 721 |
| EarI | CTCTTC | 3 cut(s) | 2, 479, 668 |
| EciI | GGCGGA | 1 cut(s) | 264 |
| Ecl136II | GAGCTC | 1 cut(s) | 147 |
| EclXI | CGGCCG | 2 cut(s) | 718, 1024 |
| Eco130I | CCWWGG | 2 cut(s) | 72, 567 |
| Eco147I | AGGCCT | 2 cut(s) | 343, 535 |
| Eco24I | GRGCYC | 2 cut(s) | 149, 1036 |
| Eco31I | GGTCTC | 1 cut(s) | 440 |
| Eco47I | GGWCC | 2 cut(s) | 112, 778 |
| Eco52I | CGGCCG | 2 cut(s) | 718, 1024 |
| Eco53kI | GAGCTC | 1 cut(s) | 147 |
| Eco57I | CTGAAG | 2 cut(s) | 107, 660 |
| Eco72I | CACGTG | 2 cut(s) | 699, 762 |
| Eco88I | CYCGRG | 1 cut(s) | 878 |
| EcoICRI | GAGCTC | 1 cut(s) | 147 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 642 |
| EcoO109I | RGGNCCY | 2 cut(s) | 1032, 1033 |
| EcoRII | CCWGG | 3 cut(s) | 530, 1011, 1035 |
| EcoT14I | CCWWGG | 2 cut(s) | 72, 567 |
| EcoT38I | GRGCYC | 2 cut(s) | 149, 1036 |
| EgeI | GGCGCC | 1 cut(s) | 1044 |
| EheI | GGCGCC | 1 cut(s) | 1044 |
| ErhI | CCWWGG | 2 cut(s) | 72, 567 |
| Esp3I | CGTCTC | 2 cut(s) | 73, 815 |
| FaeI | CATG | 2 cut(s) | 76, 571 |
| FaiI | YATR | 4 cut(s) | 74, 142, 234, 569 |
| FatI | CATG | 2 cut(s) | 72, 567 |
| FauI | CCCGC | 2 cut(s) | 334, 933 |
| FokI | GGATG | 3 cut(s) | 170, 340, 514 |
| FriOI | GRGCYC | 2 cut(s) | 149, 1036 |
| FspBI | CTAG | 2 cut(s) | 612, 633 |
| GlaI | GCGC | 1 cut(s) | 1044 |
| GsuI | CTGGAG | 1 cut(s) | 710 |
| HaeII | RGCGCY | 1 cut(s) | 1046 |
| HaeIII | GGCC | 9 cut(s) | 77, 107, 343, 535, 598, 720, 894, 1026, 1034 |
| HgaI | GACGC | 3 cut(s) | 52, 355, 521 |
| HhaI | GCGC | 1 cut(s) | 1045 |
| Hin1I | GRCGYC | 4 cut(s) | 63, 366, 513, 1043 |
| Hin1II | CATG | 2 cut(s) | 76, 571 |
| Hin6I | GCGC | 1 cut(s) | 1043 |
| HinP1I | GCGC | 1 cut(s) | 1043 |
| HincII | GTYRAC | 1 cut(s) | 735 |
| HindII | GTYRAC | 1 cut(s) | 735 |
| HinfI | GANTC | 8 cut(s) | 260, 392, 419, 563, 615, 868, 919, 995 |
| HpaI | GTTAAC | 1 cut(s) | 735 |
| HphI | GGTGA | 2 cut(s) | 518, 877 |
| Hpy166II | GTNNAC | 2 cut(s) | 351, 735 |
| Hpy188I | TCNGA | 6 cut(s) | 247, 265, 375, 418, 757, 820 |
| Hpy188III | TCNNGA | 4 cut(s) | 40, 257, 612, 923 |
| Hpy8I | GTNNAC | 2 cut(s) | 351, 735 |
| HpyAV | CCTTC | 4 cut(s) | 144, 472, 553, 898 |
| HpyCH4III | ACNGT | 5 cut(s) | 316, 386, 451, 912, 1008 |
| HpyCH4IV | ACGT | 4 cut(s) | 501, 698, 761, 824 |
| HpyCH4V | TGCA | 5 cut(s) | 198, 434, 539, 553, 985 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 160, 524, 676, 685, 988, 1040, 1049 |
| HpyF3I | CTNAG | 4 cut(s) | 345, 374, 899, 917 |
| HpySE526I | ACGT | 4 cut(s) | 501, 698, 761, 824 |
| Hsp92I | GRCGYC | 4 cut(s) | 63, 366, 513, 1043 |
| Hsp92II | CATG | 2 cut(s) | 76, 571 |
| HspAI | GCGC | 1 cut(s) | 1043 |
| KasI | GGCGCC | 1 cut(s) | 1042 |
| KroI | GCCGGC | 1 cut(s) | 894 |
| KroNI | GCCGGC | 1 cut(s) | 896 |
| KspAI | GTTAAC | 1 cut(s) | 735 |
| KspI | CCGCGG | 2 cut(s) | 111, 333 |
| Kzo9I | GATC | 4 cut(s) | 42, 51, 321, 1020 |
| LguI | GCTCTTC | 1 cut(s) | 479 |
| LmnI | GCTCC | 6 cut(s) | 15, 144, 234, 307, 469, 766 |
| Lsp1109I | GCAGC | 2 cut(s) | 446, 969 |
| LweI | GCATC | 4 cut(s) | 207, 219, 421, 536 |
| MaeI | CTAG | 2 cut(s) | 612, 633 |
| MaeII | ACGT | 4 cut(s) | 501, 698, 761, 824 |
| MaeIII | GTNAC | 2 cut(s) | 27, 386 |
| MalI | GATC | 4 cut(s) | 44, 53, 323, 1022 |
| MbiI | CCGCTC | 1 cut(s) | 769 |
| MboI | GATC | 4 cut(s) | 42, 51, 321, 1020 |
| MboII | GAAGA | 8 cut(s) | 19, 260, 496, 600, 685, 715, 760, 1030 |
| MflI | RGATCY | 1 cut(s) | 321 |
| MhlI | GDGCHC | 3 cut(s) | 149, 698, 1036 |
| Mly113I | GGCGCC | 1 cut(s) | 1043 |
| MlyI | GAGTC | 3 cut(s) | 877, 928, 1004 |
| MmeI | TCCRAC | 2 cut(s) | 780, 798 |
| MroNI | GCCGGC | 1 cut(s) | 894 |
| MroXI | GAANNNNTTC | 1 cut(s) | 270 |
| MseI | TTAA | 3 cut(s) | 498, 734, 966 |
| MspA1I | CMGCKG | 3 cut(s) | 110, 332, 437 |
| MspR9I | CCNGG | 6 cut(s) | 532, 856, 879, 880, 1013, 1037 |
| MvaI | CCWGG | 3 cut(s) | 532, 1013, 1037 |
| MvnI | CGCG | 3 cut(s) | 110, 332, 467 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 160, 524, 676, 685, 988, 1040, 1049 |
| NaeI | GCCGGC | 1 cut(s) | 896 |
| NarI | GGCGCC | 1 cut(s) | 1043 |
| NciI | CCSGG | 3 cut(s) | 856, 879, 880 |
| NcoI | CCATGG | 2 cut(s) | 72, 567 |
| NdeII | GATC | 4 cut(s) | 42, 51, 321, 1020 |
| NgoMIV | GCCGGC | 1 cut(s) | 894 |
| NheI | GCTAGC | 1 cut(s) | 632 |
| NlaIII | CATG | 2 cut(s) | 76, 571 |
| NlaIV | GGNNCC | 8 cut(s) | 99, 471, 729, 893, 909, 1033, 1034, 1044 |
| PceI | AGGCCT | 2 cut(s) | 343, 535 |
| PciSI | GCTCTTC | 1 cut(s) | 479 |
| PcsI | WCGNNNNNNNCGW | 4 cut(s) | 375, 519, 704, 881 |
| PdiI | GCCGGC | 1 cut(s) | 896 |
| PdmI | GAANNNNTTC | 1 cut(s) | 270 |
| PfeI | GAWTC | 5 cut(s) | 260, 392, 419, 563, 615 |
| Pfl23II | CGTACG | 1 cut(s) | 423 |
| PflFI | GACNNNGTC | 2 cut(s) | 116, 856 |
| PinAI | ACCGGT | 1 cut(s) | 601 |
| PleI | GAGTC | 3 cut(s) | 876, 927, 1003 |
| PluTI | GGCGCC | 1 cut(s) | 1046 |
| PmaCI | CACGTG | 2 cut(s) | 699, 762 |
| PmlI | CACGTG | 2 cut(s) | 699, 762 |
| PpsI | GAGTC | 3 cut(s) | 876, 927, 1003 |
| Ppu21I | YACGTR | 2 cut(s) | 699, 762 |
| Psp124BI | GAGCTC | 1 cut(s) | 149 |
| Psp6I | CCWGG | 3 cut(s) | 530, 1011, 1035 |
| PspCI | CACGTG | 2 cut(s) | 699, 762 |
| PspGI | CCWGG | 3 cut(s) | 530, 1011, 1035 |
| PspLI | CGTACG | 1 cut(s) | 423 |
| PspN4I | GGNNCC | 8 cut(s) | 99, 471, 729, 893, 909, 1033, 1034, 1044 |
| PspOMI | GGGCCC | 1 cut(s) | 1032 |
| PspPI | GGNCC | 5 cut(s) | 112, 778, 892, 1032, 1033 |
| PstI | CTGCAG | 1 cut(s) | 987 |
| PsuI | RGATCY | 1 cut(s) | 321 |
| PsyI | GACNNNGTC | 2 cut(s) | 116, 856 |
| RsaI | GTAC | 4 cut(s) | 350, 383, 425, 1010 |
| RsaNI | GTAC | 4 cut(s) | 349, 382, 424, 1009 |
| SacI | GAGCTC | 1 cut(s) | 149 |
| SacII | CCGCGG | 2 cut(s) | 111, 333 |
| SapI | GCTCTTC | 1 cut(s) | 479 |
| SaqAI | TTAA | 3 cut(s) | 498, 734, 966 |
| Sau3AI | GATC | 4 cut(s) | 42, 51, 321, 1020 |
| Sau96I | GGNCC | 5 cut(s) | 112, 778, 892, 1032, 1033 |
| SchI | GAGTC | 3 cut(s) | 877, 928, 1004 |
| ScrFI | CCNGG | 6 cut(s) | 532, 856, 879, 880, 1013, 1037 |
| SduI | GDGCHC | 3 cut(s) | 149, 698, 1036 |
| SfaNI | GCATC | 4 cut(s) | 207, 219, 421, 536 |
| SfcI | CTRYAG | 1 cut(s) | 983 |
| SfoI | GGCGCC | 1 cut(s) | 1044 |
| Sfr303I | CCGCGG | 2 cut(s) | 111, 333 |
| SgrAI | CRCCGGYG | 2 cut(s) | 59, 862 |
| SgrBI | CCGCGG | 2 cut(s) | 111, 333 |
| SinI | GGWCC | 2 cut(s) | 112, 778 |
| SmaI | CCCGGG | 1 cut(s) | 880 |
| SmlI | CTYRAG | 1 cut(s) | 38 |
| SmoI | CTYRAG | 1 cut(s) | 38 |
| SseBI | AGGCCT | 2 cut(s) | 343, 535 |
| SspDI | GGCGCC | 1 cut(s) | 1042 |
| SspMI | CTAG | 2 cut(s) | 612, 633 |
| SstI | GAGCTC | 1 cut(s) | 149 |
| StuI | AGGCCT | 2 cut(s) | 343, 535 |
| StyD4I | CCNGG | 6 cut(s) | 530, 854, 877, 878, 1011, 1035 |
| StyI | CCWWGG | 2 cut(s) | 72, 567 |
| TaaI | ACNGT | 5 cut(s) | 316, 386, 451, 912, 1008 |
| TaiI | ACGT | 4 cut(s) | 504, 701, 764, 827 |
| TaqI | TCGA | 3 cut(s) | 5, 311, 665 |
| TatI | WGTACW | 1 cut(s) | 348 |
| TauI | GCSGC | 9 cut(s) | 110, 280, 283, 332, 440, 518, 521, 772, 1043 |
| TfiI | GAWTC | 5 cut(s) | 260, 392, 419, 563, 615 |
| Tru1I | TTAA | 3 cut(s) | 498, 734, 966 |
| Tru9I | TTAA | 3 cut(s) | 498, 734, 966 |
| TscAI | CASTG | 1 cut(s) | 90 |
| TseI | GCWGC | 2 cut(s) | 434, 982 |
| TspDTI | ATGAA | 2 cut(s) | 824, 943 |
| TspGWI | ACGGA | 3 cut(s) | 441, 556, 728 |
| TspMI | CCCGGG | 1 cut(s) | 878 |
| TspRI | CASTG | 1 cut(s) | 90 |
| Tth111I | GACNNNGTC | 2 cut(s) | 116, 856 |
| VpaK11BI | GGWCC | 2 cut(s) | 112, 778 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 642 |
| XbaI | TCTAGA | 1 cut(s) | 611 |
| XmaI | CCCGGG | 1 cut(s) | 878 |
| XmnI | GAANNNNTTC | 1 cut(s) | 270 |
| XspI | CTAG | 2 cut(s) | 612, 633 |