AT5G54530

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012173
Physical Location & Seq
Forward (+)
22152724 .. 22154466
1743 bp
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UTR
Exon/CDS
Intron
AT5G54530.1

Sequence Viewer

Length: 486 bp
ATGAACAAACTCATCTCCCGTATGATCCTTCTCCTCACCACTCTCCGCCTCTCTTTATCCCTCTCCTCGCCGTCGTACCCAACGGTCCACGACGTTCTCCGATCAGAAGGTCTCCCGGCGGGACTTTTACCTCAAGAGGTTGATTCTTATATTCTTCACAACGACGGTCGTCTCGAGGTTTTCCTCGCGGCGCCGTGTTACGCTAAATTCGAGACGAACGTGCACTTTGAAGCCGTCGTAAGAGGGAATCTGAGCTATGGGAGCCTCGTCGGAGTAGAAGGGCTATCGCAAAAAGAGCTCTTCTTGTGGCTTCAGGTGAAAGATATCGTCGTCGAGAATCCAAATTCCGGCGTCATTGTGTTTGATATCGGCGTTGCTTTTAAACAGCTTTCGCTTTCTCTCTTTGAAGACCCTCCCAAGTGTAAACCTGACGGTGTTTTGAAGAAAAAGATGAGAAGAGATAGAGGATTTGAAGCTCAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

161

Amino Acids

17.96

Weight (kDa)

7.77

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 29 - 136 1.6e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54530
fragaria_vesca FvH4_3g21500
malus_domestica MD01G1090100.v1.1 MD03G1215600.v1.1 MD11G1230200.v1.1
prunus_persica Prupe.4G197200_v2.0.a1
pyrus_communis pycom03g16400 pycom11g20400
rosa_chinensis RchiOBHm_Chr5g0036931
rosa_laevigata RLG00000033735
rosa_multiflora Rmu_sc0007663.1_g000018
rosa_roxburghii Rroxscaffold_1G00044020
rosa_rugosa Rorug05G0159000 Rorug05G0159100
rosa_samantha Rh5AG251300 Rh5BG252800 Rh5CG285400 Rh5DG261400
rosa_wichuraiana Rw5G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 190
AccII CGCG 1 cut(s) 188
AciI CCGC 3 cut(s) 46, 119, 188
AclWI GGATC 1 cut(s) 19
AcsI RAATTY 2 cut(s) 206, 343
AcuI CTGAAG 1 cut(s) 296
AcyI GRCGYC 2 cut(s) 191, 351
AfaI GTAC 1 cut(s) 77
AfiI CCNNNNNNNGG 1 cut(s) 347
AgsI TTSAA 4 cut(s) 230, 407, 442, 473
AluBI AGCT 4 cut(s) 255, 298, 388, 476
AluI AGCT 4 cut(s) 255, 298, 388, 476
Alw21I GWGCWC 2 cut(s) 225, 300
Alw26I GTCTC 3 cut(s) 116, 176, 206
Alw44I GTGCAC 1 cut(s) 221
AlwI GGATC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 173
ApaLI GTGCAC 1 cut(s) 221
ApoI RAATTY 2 cut(s) 206, 343
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 1 cut(s) 85
AsuC2I CCSGG 1 cut(s) 116
AsuHPI GGTGA 2 cut(s) 28, 328
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 1 cut(s) 85
BaeGI GKGCMC 1 cut(s) 225
BanI GGYRCC 1 cut(s) 190
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 414
Bbv12I GWGCWC 2 cut(s) 225, 300
BceAI ACGGC 3 cut(s) 55, 178, 218
BcnI CCSGG 1 cut(s) 116
BcoDI GTCTC 3 cut(s) 116, 176, 206
BfoI RGCGCY 1 cut(s) 194
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 1 cut(s) 116
Bme18I GGWCC 1 cut(s) 85
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 1 cut(s) 85
BmiI GGNNCC 2 cut(s) 192, 263
BmrFI CCNGG 1 cut(s) 116
BoxI GACNNNNGTC 1 cut(s) 168
BpiI GAAGAC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 116
BsaHI GRCGYC 2 cut(s) 191, 351
BsaI GGTCTC 1 cut(s) 116
Bsc4I CCNNNNNNNGG 1 cut(s) 347
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMII CTCAG 1 cut(s) 242
BseRI GAGGAG 2 cut(s) 23, 55
BseSI GKGCMC 1 cut(s) 225
Bsh1236I CGCG 1 cut(s) 188
Bsh1285I CGRYCG 1 cut(s) 169
BshNI GGYRCC 1 cut(s) 190
BsiEI CGRYCG 1 cut(s) 169
BsiHKAI GWGCWC 2 cut(s) 225, 300
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 2 cut(s) 116, 348
BslFI GGGAC 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 3 cut(s) 116, 176, 206
BsmBI CGTCTC 2 cut(s) 176, 206
BsmFI GGGAC 1 cut(s) 135
Bso31I GGTCTC 1 cut(s) 116
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 2 cut(s) 225, 300
Bsp143I GATC 2 cut(s) 24, 101
BspACI CCGC 3 cut(s) 46, 119, 188
BspCNI CTCAG 1 cut(s) 243
BspFNI CGCG 1 cut(s) 188
BspLI GGNNCC 2 cut(s) 192, 263
BspPI GGATC 1 cut(s) 19
BspQI GCTCTTC 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 190
BspTNI GGTCTC 1 cut(s) 116
BssMI GATC 2 cut(s) 24, 101
BssNI GRCGYC 2 cut(s) 191, 351
Bst4CI ACNGT 3 cut(s) 85, 167, 434
Bst6I CTCTTC 2 cut(s) 305, 451
BstACI GRCGYC 2 cut(s) 191, 351
BstDEI CTNAG 2 cut(s) 251, 477
BstFNI CGCG 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 194
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 2 cut(s) 27, 104
BstMAI GTCTC 3 cut(s) 116, 176, 206
BstMBI GATC 2 cut(s) 24, 101
BstMCI CGRYCG 1 cut(s) 169
BstMWI GCNNNNNNNGC 2 cut(s) 261, 295
BstPAI GACNNNNGTC 1 cut(s) 168
BstSCI CCNGG 1 cut(s) 114
BstSLI GKGCMC 1 cut(s) 225
BstUI CGCG 1 cut(s) 188
BstV2I GAAGAC 1 cut(s) 414
CfoI GCGC 1 cut(s) 193
Cfr13I GGNCC 1 cut(s) 85
CseI GACGC 1 cut(s) 340
Csp6I GTAC 1 cut(s) 76
CviJI RGCY 8 cut(s) 233, 255, 264, 283, 298, 310, 388, 476
CviKI_1 RGCY 8 cut(s) 233, 255, 264, 283, 298, 310, 388, 476
CviQI GTAC 1 cut(s) 76
DdeI CTNAG 2 cut(s) 251, 477
DinI GGCGCC 1 cut(s) 192
DpnI GATC 2 cut(s) 26, 103
DpnII GATC 2 cut(s) 24, 101
DraI TTTAAA 1 cut(s) 382
Eam1104I CTCTTC 2 cut(s) 305, 451
EarI CTCTTC 2 cut(s) 305, 451
EciI GGCGGA 1 cut(s) 35
Ecl136II GAGCTC 1 cut(s) 298
Eco24I GRGCYC 1 cut(s) 300
Eco31I GGTCTC 1 cut(s) 116
Eco32I GATATC 2 cut(s) 325, 367
Eco47I GGWCC 1 cut(s) 85
Eco53kI GAGCTC 1 cut(s) 298
Eco57I CTGAAG 1 cut(s) 296
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 298
EcoRV GATATC 2 cut(s) 325, 367
EcoT38I GRGCYC 1 cut(s) 300
EgeI GGCGCC 1 cut(s) 192
EheI GGCGCC 1 cut(s) 192
Esp3I CGTCTC 2 cut(s) 176, 206
FaiI YATR 3 cut(s) 23, 150, 258
FaqI GGGAC 1 cut(s) 135
FauI CCCGC 1 cut(s) 112
Fnu4HI GCNGC 1 cut(s) 189
FriOI GRGCYC 1 cut(s) 300
Fsp4HI GCNGC 1 cut(s) 189
GlaI GCGC 1 cut(s) 192
GluI GCNGC 1 cut(s) 189
HaeII RGCGCY 1 cut(s) 194
HapII CCGG 2 cut(s) 116, 348
HgaI GACGC 1 cut(s) 340
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 2 cut(s) 191, 351
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HinfI GANTC 3 cut(s) 143, 247, 337
HpaII CCGG 2 cut(s) 116, 348
HphI GGTGA 2 cut(s) 28, 328
Hpy166II GTNNAC 3 cut(s) 88, 223, 425
Hpy188I TCNGA 5 cut(s) 101, 106, 252, 272, 480
Hpy188III TCNNGA 4 cut(s) 134, 173, 211, 334
Hpy8I GTNNAC 3 cut(s) 88, 223, 425
Hpy99I CGWCG 7 cut(s) 76, 95, 167, 239, 272, 332, 335
HpyAV CCTTC 3 cut(s) 38, 101, 272
HpyCH4III ACNGT 3 cut(s) 85, 167, 434
HpyCH4IV ACGT 2 cut(s) 93, 219
HpyCH4V TGCA 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 2 cut(s) 261, 295
HpyF3I CTNAG 2 cut(s) 251, 477
HpySE526I ACGT 2 cut(s) 93, 219
Hsp92I GRCGYC 2 cut(s) 191, 351
HspAI GCGC 1 cut(s) 191
KasI GGCGCC 1 cut(s) 190
Kzo9I GATC 2 cut(s) 24, 101
LguI GCTCTTC 1 cut(s) 305
LmnI GCTCC 1 cut(s) 261
LpnPI CCDG 4 cut(s) 129, 299, 361, 441
MaeII ACGT 2 cut(s) 93, 219
MaeIII GTNAC 1 cut(s) 197
MalI GATC 2 cut(s) 26, 103
MboI GATC 2 cut(s) 24, 101
MboII GAAGA 5 cut(s) 146, 292, 419, 454, 468
MhlI GDGCHC 2 cut(s) 225, 300
MluCI AATT 2 cut(s) 206, 343
Mly113I GGCGCC 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 250
MseI TTAA 1 cut(s) 381
MspI CCGG 2 cut(s) 116, 348
MspR9I CCNGG 1 cut(s) 116
MvnI CGCG 1 cut(s) 188
MwoI GCNNNNNNNGC 2 cut(s) 261, 295
NarI GGCGCC 1 cut(s) 191
NciI CCSGG 1 cut(s) 116
NdeII GATC 2 cut(s) 24, 101
NlaIV GGNNCC 2 cut(s) 192, 263
PaeR7I CTCGAG 1 cut(s) 173
PciSI GCTCTTC 1 cut(s) 305
PcsI WCGNNNNNNNCGW 3 cut(s) 171, 207, 216
PfeI GAWTC 3 cut(s) 143, 247, 337
PkrI GCNGC 1 cut(s) 190
PluTI GGCGCC 1 cut(s) 194
PshAI GACNNNNGTC 1 cut(s) 168
Psp124BI GAGCTC 1 cut(s) 300
PspN4I GGNNCC 2 cut(s) 192, 263
PspPI GGNCC 1 cut(s) 85
RsaI GTAC 1 cut(s) 77
RsaNI GTAC 1 cut(s) 76
SacI GAGCTC 1 cut(s) 300
SapI GCTCTTC 1 cut(s) 305
SaqAI TTAA 1 cut(s) 381
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 2 cut(s) 24, 101
Sau96I GGNCC 1 cut(s) 85
ScrFI CCNGG 1 cut(s) 116
SduI GDGCHC 2 cut(s) 225, 300
SfoI GGCGCC 1 cut(s) 192
Sfr274I CTCGAG 1 cut(s) 173
SinI GGWCC 1 cut(s) 85
SlaI CTCGAG 1 cut(s) 173
SmlI CTYRAG 2 cut(s) 132, 173
SmoI CTYRAG 2 cut(s) 132, 173
Sse9I AATT 2 cut(s) 206, 343
SsiI CCGC 3 cut(s) 46, 119, 188
SspDI GGCGCC 1 cut(s) 190
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 114
TaaI ACNGT 3 cut(s) 85, 167, 434
TaiI ACGT 2 cut(s) 96, 222
TaqI TCGA 3 cut(s) 174, 210, 333
TasI AATT 2 cut(s) 206, 343
TauI GCSGC 1 cut(s) 191
TfiI GAWTC 3 cut(s) 143, 247, 337
Tru1I TTAA 1 cut(s) 381
Tru9I TTAA 1 cut(s) 381
TspDTI ATGAA 1 cut(s) 17
VneI GTGCAC 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 85
XapI RAATTY 2 cut(s) 206, 343
XhoI CTCGAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.