FvH4_3g21500

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012173
Physical Location & Seq
Forward (+)
14619165 .. 14621153
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21500.t1

Sequence Viewer

Length: 495 bp
ATGTCCTTCTTCACACTACTCCTCCCATGGACTCTTCTCCTTACCGTTCTACTCTCTCTACCCCTCTCTCTCTCCTCCTCTTCCTCCATCCATGACCTCCTCCGCTCGCAGGGCCTGCCAGCTGGTCTCCTCCCTAAGGAAGTCAAGTCCTACTCACTCTCCGACGACGGCCTCCTCCAAGTCTTCCTCGACGCTCCGTGCCTCACCAAATTTGAGAACCGGGTTTTCTTCGACAGCGTCGTGAGGGCCAACCTCAGCTACGGAAGTCTCATCGGAGTTGAGGGTTTGACTCAGGAAGAGCTCTTTCTGTGGCTGCCGGTGAAGGACATCATCGTTGATGATCCGGGATCCGGTCTCATACTCTTCGACATTGGAGTAGCTCATAAACAGCTTTCTCTGTCGCTCTTTGAAGATCCTCCTTCCTGTAAACCCTCAGGTGCAGTAGTGAAGAATCATGTGAAGAAGCAGGGAAAAGGGTTTGAAGCTCTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

165

Amino Acids

17.91

Weight (kDa)

5.54

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 30 - 137 1.7e-27 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54530
fragaria_vesca FvH4_3g21500
malus_domestica MD01G1090100.v1.1 MD03G1215600.v1.1 MD11G1230200.v1.1
prunus_persica Prupe.4G197200_v2.0.a1
pyrus_communis pycom03g16400 pycom11g20400
rosa_chinensis RchiOBHm_Chr5g0036931
rosa_laevigata RLG00000033735
rosa_multiflora Rmu_sc0007663.1_g000018
rosa_roxburghii Rroxscaffold_1G00044020
rosa_rugosa Rorug05G0159000 Rorug05G0159100
rosa_samantha Rh5AG251300 Rh5BG252800 Rh5CG285400 Rh5DG261400
rosa_wichuraiana Rw5G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 105
AciI CCGC 1 cut(s) 103
AclWI GGATC 4 cut(s) 335, 342, 355, 407
AcsI RAATTY 1 cut(s) 209
AfiI CCNNNNNNNGG 3 cut(s) 109, 136, 350
AgsI TTSAA 2 cut(s) 410, 482
AluBI AGCT 6 cut(s) 122, 258, 301, 380, 391, 485
AluI AGCT 6 cut(s) 122, 258, 301, 380, 391, 485
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 3 cut(s) 131, 272, 359
AlwI GGATC 4 cut(s) 335, 342, 355, 407
AlwNI CAGNNNCTG 1 cut(s) 115
AoxI GGCC 3 cut(s) 112, 169, 246
ApeKI GCWGC 1 cut(s) 313
ApoI RAATTY 1 cut(s) 209
AspS9I GGNCC 2 cut(s) 112, 246
AsuC2I CCSGG 2 cut(s) 221, 345
AsuHPI GGTGA 2 cut(s) 196, 331
AxyI CCTNAGG 2 cut(s) 135, 433
BamHI GGATCC 1 cut(s) 347
BanII GRGCYC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 175
Bbv12I GWGCWC 1 cut(s) 303
BbvCI CCTCAGC 1 cut(s) 254
BbvI GCAGC 1 cut(s) 300
BccI CCATC 1 cut(s) 95
BceAI ACGGC 1 cut(s) 184
BcnI CCSGG 2 cut(s) 221, 345
BcoDI GTCTC 3 cut(s) 131, 272, 359
BisI GCNGC 1 cut(s) 314
BlsI GCNGC 1 cut(s) 315
Bme1390I CCNGG 2 cut(s) 221, 345
BmgT120I GGNCC 2 cut(s) 112, 246
BmiI GGNNCC 1 cut(s) 349
BmrFI CCNGG 2 cut(s) 221, 345
BpiI GAAGAC 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 254
BpuMI CCSGG 2 cut(s) 221, 345
BsaI GGTCTC 2 cut(s) 131, 359
BsaJI CCNNGG 1 cut(s) 26
BsaWI WCCGGW 1 cut(s) 350
BsaXI ACNNNNNCTCC 4 cut(s) 6, 36, 143, 173
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 136, 350
Bse118I RCCGGY 1 cut(s) 316
Bse21I CCTNAGG 2 cut(s) 135, 433
BseDI CCNNGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 87
BseLI CCNNNNNNNGG 3 cut(s) 109, 136, 350
BseMII CTCAG 4 cut(s) 268, 305, 447, 479
BseRI GAGGAG 6 cut(s) 11, 64, 67, 89, 119, 164
BseXI GCAGC 1 cut(s) 300
BsgI GTGCAG 1 cut(s) 459
BshFI GGCC 3 cut(s) 114, 171, 248
BsiHKAI GWGCWC 1 cut(s) 303
BsiSI CCGG 4 cut(s) 220, 317, 344, 351
BslI CCNNNNNNNGG 3 cut(s) 109, 136, 350
BsmAI GTCTC 3 cut(s) 131, 272, 359
BsnI GGCC 3 cut(s) 114, 171, 248
Bso31I GGTCTC 2 cut(s) 131, 359
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 3 cut(s) 340, 347, 412
Bsp19I CCATGG 1 cut(s) 26
BspACI CCGC 1 cut(s) 103
BspANI GGCC 3 cut(s) 114, 171, 248
BspCNI CTCAG 4 cut(s) 267, 304, 446, 480
BspLI GGNNCC 1 cut(s) 349
BspPI GGATC 4 cut(s) 335, 342, 355, 407
BspQI GCTCTTC 1 cut(s) 291
BspTNI GGTCTC 2 cut(s) 131, 359
BsrBI CCGCTC 1 cut(s) 105
BsrFI RCCGGY 1 cut(s) 316
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 1 cut(s) 26
BssMI GATC 3 cut(s) 340, 347, 412
BssT1I CCWWGG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 4 cut(s) 39, 85, 291, 368
BstAPI GCANNNNNTGC 1 cut(s) 115
BstC8I GCNNGC 3 cut(s) 107, 116, 120
BstDEI CTNAG 5 cut(s) 135, 254, 291, 433, 488
BstDSI CCRYGG 1 cut(s) 26
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 1 cut(s) 87
BstKTI GATC 3 cut(s) 343, 350, 415
BstMAI GTCTC 3 cut(s) 131, 272, 359
BstMBI GATC 3 cut(s) 340, 347, 412
BstMWI GCNNNNNNNGC 2 cut(s) 111, 115
BstSCI CCNGG 2 cut(s) 219, 343
BstV1I GCAGC 1 cut(s) 300
BstV2I GAAGAC 1 cut(s) 175
BstX2I RGATCY 2 cut(s) 347, 412
BstYI RGATCY 2 cut(s) 347, 412
Bsu36I CCTNAGG 2 cut(s) 135, 433
BsuRI GGCC 3 cut(s) 114, 171, 248
BtgI CCRYGG 1 cut(s) 26
BtsCI GGATG 1 cut(s) 87
Cac8I GCNNGC 3 cut(s) 107, 116, 120
CaiI CAGNNNCTG 1 cut(s) 115
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 2 cut(s) 112, 246
CseI GACGC 2 cut(s) 200, 226
CviAII CATG 3 cut(s) 27, 92, 455
DdeI CTNAG 5 cut(s) 135, 254, 291, 433, 488
DpnI GATC 3 cut(s) 342, 349, 414
DpnII GATC 3 cut(s) 340, 347, 412
Eam1104I CTCTTC 4 cut(s) 39, 85, 291, 368
EarI CTCTTC 4 cut(s) 39, 85, 291, 368
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 1 cut(s) 26
Eco24I GRGCYC 1 cut(s) 303
Eco31I GGTCTC 2 cut(s) 131, 359
Eco53kI GAGCTC 1 cut(s) 301
Eco81I CCTNAGG 2 cut(s) 135, 433
EcoICRI GAGCTC 1 cut(s) 301
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoO109I RGGNCCY 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 3 cut(s) 30, 95, 458
FaiI YATR 5 cut(s) 28, 93, 359, 384, 456
FatI CATG 3 cut(s) 26, 91, 454
Fnu4HI GCNGC 1 cut(s) 314
FokI GGATG 1 cut(s) 74
FriOI GRGCYC 1 cut(s) 303
Fsp4HI GCNGC 1 cut(s) 314
GluI GCNGC 1 cut(s) 314
HaeIII GGCC 3 cut(s) 114, 171, 248
HapII CCGG 4 cut(s) 220, 317, 344, 351
HgaI GACGC 2 cut(s) 200, 226
Hin1II CATG 3 cut(s) 30, 95, 458
HinfI GANTC 3 cut(s) 31, 289, 451
HpaII CCGG 4 cut(s) 220, 317, 344, 351
HphI GGTGA 2 cut(s) 196, 331
Hpy166II GTNNAC 1 cut(s) 428
Hpy188I TCNGA 3 cut(s) 163, 275, 489
Hpy188III TCNNGA 2 cut(s) 241, 293
Hpy8I GTNNAC 1 cut(s) 428
Hpy99I CGWCG 4 cut(s) 167, 170, 194, 242
HpyAV CCTTC 3 cut(s) 16, 316, 429
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4V TGCA 1 cut(s) 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 115
HpyF3I CTNAG 5 cut(s) 135, 254, 291, 433, 488
Hsp92II CATG 3 cut(s) 30, 95, 458
Kzo9I GATC 3 cut(s) 340, 347, 412
LguI GCTCTTC 1 cut(s) 291
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 1 cut(s) 300
MalI GATC 3 cut(s) 342, 349, 414
MbiI CCGCTC 1 cut(s) 105
MboI GATC 3 cut(s) 340, 347, 412
MboII GAAGA 9 cut(s) 26, 72, 175, 220, 308, 355, 422, 460, 472
MflI RGATCY 2 cut(s) 347, 412
MhlI GDGCHC 1 cut(s) 303
MluCI AATT 1 cut(s) 209
MlyI GAGTC 2 cut(s) 25, 283
MmeI TCCRAC 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 4 cut(s) 220, 317, 344, 351
MspR9I CCNGG 2 cut(s) 221, 345
MwoI GCNNNNNNNGC 2 cut(s) 111, 115
NciI CCSGG 2 cut(s) 221, 345
NcoI CCATGG 1 cut(s) 26
NdeII GATC 3 cut(s) 340, 347, 412
NlaIII CATG 3 cut(s) 30, 95, 458
NlaIV GGNNCC 1 cut(s) 349
PciSI GCTCTTC 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 451
PflFI GACNNNGTC 1 cut(s) 236
PfoI TCCNGGA 1 cut(s) 343
PkrI GCNGC 1 cut(s) 315
PleI GAGTC 2 cut(s) 25, 283
PpsI GAGTC 2 cut(s) 25, 283
Psp124BI GAGCTC 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 349
PspPI GGNCC 2 cut(s) 112, 246
PstNI CAGNNNCTG 1 cut(s) 115
PsuI RGATCY 2 cut(s) 347, 412
PsyI GACNNNGTC 1 cut(s) 236
PvuII CAGCTG 1 cut(s) 122
SacI GAGCTC 1 cut(s) 303
SapI GCTCTTC 1 cut(s) 291
SatI GCNGC 1 cut(s) 314
Sau3AI GATC 3 cut(s) 340, 347, 412
Sau96I GGNCC 2 cut(s) 112, 246
SchI GAGTC 2 cut(s) 25, 283
ScrFI CCNGG 2 cut(s) 221, 345
SduI GDGCHC 1 cut(s) 303
SetI ASST 9 cut(s) 99, 124, 255, 260, 303, 382, 393, 439, 487
Sse9I AATT 1 cut(s) 209
SsiI CCGC 1 cut(s) 103
SstI GAGCTC 1 cut(s) 303
StyD4I CCNGG 2 cut(s) 219, 343
StyI CCWWGG 1 cut(s) 26
TaaI ACNGT 1 cut(s) 46
TaqI TCGA 3 cut(s) 189, 231, 366
TasI AATT 1 cut(s) 209
TfiI GAWTC 1 cut(s) 451
TseI GCWGC 1 cut(s) 313
TspGWI ACGGA 2 cut(s) 186, 276
Tth111I GACNNNGTC 1 cut(s) 236
XagI CCTNNNNNAGG 1 cut(s) 134
XapI RAATTY 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.