MD03G1215600.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012173
Physical Location & Seq
Reverse (-)
29591534 .. 29593584
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1215600.v1.1.491

Sequence Viewer

Length: 504 bp
ATGTCCCTGTTAAACACCCACTTCTTCTCCTCCTTCCTCAGAGTCTTTCCATGGACCTTTCTCCTCCTTGTCTCTCTTCCCCTCTCTCTCTCCTCCACCAACATCCACGACCTCCTTATCTCCCAGGGTCTGCCACCTGGCCTCCTCCCAAAGGAGGTCAAGTCCTACACTCTCTCAGACAATGGGGAGCTCCAGGTCTTCCTTGATGCCCCATGCCTCACAAAATATGAGAACAGGGTTTTCTTTGAGAGCGTGGTGAGGGCAAATCTCAGCTATGGCAGCCTCATTGGGGTTCAGGGTTTGTCTCAGGAGGAGCTCTTTCTGTGGCTGCCGGTCAAAGACATCATTGTTGATGATCCAAGATCAGGTCTCATCCTCTTTGACATTGGGGTTGCTCACAAACAGTTTTCCCTCTCACTCTTTGAGGATCCCCCTGACTGTAAACCCTCAGGTGTGTTGAAGAATCATGTGAGGAAGGAGAAAGGCTTTTGGGGTCTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

168

Amino Acids

18.81

Weight (kDa)

6.06

Isoelectric Point (pI)

40.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 36 - 142 4.6e-28 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54530
fragaria_vesca FvH4_3g21500
malus_domestica MD01G1090100.v1.1 MD03G1215600.v1.1 MD11G1230200.v1.1
prunus_persica Prupe.4G197200_v2.0.a1
pyrus_communis pycom03g16400 pycom11g20400
rosa_chinensis RchiOBHm_Chr5g0036931
rosa_laevigata RLG00000033735
rosa_multiflora Rmu_sc0007663.1_g000018
rosa_roxburghii Rroxscaffold_1G00044020
rosa_rugosa Rorug05G0159000 Rorug05G0159100
rosa_samantha Rh5AG251300 Rh5BG252800 Rh5CG285400 Rh5DG261400
rosa_wichuraiana Rw5G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 350, 422, 435
AfiI CCNNNNNNNGG 4 cut(s) 151, 154, 289, 365
AgsI TTSAA 1 cut(s) 460
AjnI CCWGG 3 cut(s) 123, 136, 192
AluBI AGCT 3 cut(s) 190, 273, 316
AluI AGCT 3 cut(s) 190, 273, 316
Alw21I GWGCWC 2 cut(s) 192, 318
Alw26I GTCTC 3 cut(s) 76, 309, 374
AlwI GGATC 3 cut(s) 350, 422, 435
AlwNI CAGNNNCTG 1 cut(s) 130
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 2 cut(s) 279, 328
AspS9I GGNCC 1 cut(s) 54
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 1 cut(s) 54
AxyI CCTNAGG 1 cut(s) 448
BamHI GGATCC 1 cut(s) 427
BanII GRGCYC 2 cut(s) 192, 318
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 2 cut(s) 192, 318
BbvI GCAGC 2 cut(s) 291, 315
BciT130I CCWGG 3 cut(s) 125, 138, 194
BcoDI GTCTC 3 cut(s) 76, 309, 374
BisI GCNGC 2 cut(s) 280, 329
BlsI GCNGC 2 cut(s) 281, 330
Bme1390I CCNGG 3 cut(s) 125, 138, 194
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 54
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 3 cut(s) 125, 138, 194
BmsI GCATC 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 190
BpmI CTGGAG 1 cut(s) 176
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 50, 123, 124
BsaXI ACNNNNNCTCC 6 cut(s) 11, 41, 126, 146, 156, 176
Bsc4I CCNNNNNNNGG 4 cut(s) 151, 154, 289, 365
Bse118I RCCGGY 1 cut(s) 331
Bse21I CCTNAGG 1 cut(s) 448
BseBI CCWGG 3 cut(s) 125, 138, 194
BseDI CCNNGG 3 cut(s) 50, 123, 124
BseGI GGATG 2 cut(s) 102, 372
BseLI CCNNNNNNNGG 4 cut(s) 151, 154, 289, 365
BseMII CTCAG 6 cut(s) 52, 189, 283, 320, 462, 488
BseRI GAGGAG 5 cut(s) 19, 53, 82, 134, 326
BseXI GCAGC 2 cut(s) 291, 315
BshFI GGCC 1 cut(s) 141
BsiHKAI GWGCWC 2 cut(s) 192, 318
BsiSI CCGG 1 cut(s) 332
BslI CCNNNNNNNGG 4 cut(s) 151, 154, 289, 365
BsmAI GTCTC 3 cut(s) 76, 309, 374
BsnI GGCC 1 cut(s) 141
Bso31I GGTCTC 1 cut(s) 374
Bsp1286I GDGCHC 2 cut(s) 192, 318
Bsp143I GATC 3 cut(s) 355, 362, 427
Bsp19I CCATGG 1 cut(s) 50
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 6 cut(s) 51, 188, 282, 319, 461, 489
BspLI GGNNCC 1 cut(s) 429
BspPI GGATC 3 cut(s) 350, 422, 435
BspTNI GGTCTC 1 cut(s) 374
BsrFI RCCGGY 1 cut(s) 331
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 3 cut(s) 50, 123, 124
BssMI GATC 3 cut(s) 355, 362, 427
BssT1I CCWWGG 1 cut(s) 50
Bst2UI CCWGG 3 cut(s) 125, 138, 194
Bst4CI ACNGT 2 cut(s) 405, 440
Bst6I CTCTTC 1 cut(s) 81
BstDEI CTNAG 6 cut(s) 38, 175, 269, 306, 448, 497
BstDSI CCRYGG 1 cut(s) 50
BstENI CCTNNNNNAGG 1 cut(s) 149
BstF5I GGATG 2 cut(s) 102, 372
BstKTI GATC 3 cut(s) 358, 365, 430
BstMAI GTCTC 3 cut(s) 76, 309, 374
BstMBI GATC 3 cut(s) 355, 362, 427
BstMWI GCNNNNNNNGC 1 cut(s) 279
BstNI CCWGG 3 cut(s) 125, 138, 194
BstSCI CCNGG 3 cut(s) 123, 136, 192
BstV1I GCAGC 2 cut(s) 291, 315
BstV2I GAAGAC 1 cut(s) 190
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
Bsu36I CCTNAGG 1 cut(s) 448
BsuRI GGCC 1 cut(s) 141
BtgI CCRYGG 1 cut(s) 50
BtsCI GGATG 2 cut(s) 102, 372
CaiI CAGNNNCTG 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 54
CviAII CATG 3 cut(s) 51, 213, 467
CviJI RGCY 7 cut(s) 141, 190, 273, 282, 316, 328, 486
CviKI_1 RGCY 7 cut(s) 141, 190, 273, 282, 316, 328, 486
DdeI CTNAG 6 cut(s) 38, 175, 269, 306, 448, 497
DpnI GATC 3 cut(s) 357, 364, 429
DpnII GATC 3 cut(s) 355, 362, 427
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
Ecl136II GAGCTC 2 cut(s) 190, 316
Eco130I CCWWGG 1 cut(s) 50
Eco24I GRGCYC 2 cut(s) 192, 318
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 1 cut(s) 54
Eco53kI GAGCTC 2 cut(s) 190, 316
Eco81I CCTNAGG 1 cut(s) 448
EcoICRI GAGCTC 2 cut(s) 190, 316
EcoNI CCTNNNNNAGG 1 cut(s) 149
EcoRII CCWGG 3 cut(s) 123, 136, 192
EcoT14I CCWWGG 1 cut(s) 50
EcoT38I GRGCYC 2 cut(s) 192, 318
ErhI CCWWGG 1 cut(s) 50
FaeI CATG 3 cut(s) 54, 216, 470
FaiI YATR 5 cut(s) 52, 214, 228, 276, 468
FatI CATG 3 cut(s) 50, 212, 466
Fnu4HI GCNGC 2 cut(s) 280, 329
FokI GGATG 2 cut(s) 89, 359
FriOI GRGCYC 2 cut(s) 192, 318
Fsp4HI GCNGC 2 cut(s) 280, 329
GluI GCNGC 2 cut(s) 280, 329
GsuI CTGGAG 1 cut(s) 176
HaeIII GGCC 1 cut(s) 141
HapII CCGG 1 cut(s) 332
Hin1II CATG 3 cut(s) 54, 216, 470
HinfI GANTC 2 cut(s) 42, 463
HpaII CCGG 1 cut(s) 332
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 3 cut(s) 41, 178, 498
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 1 cut(s) 443
HpyAV CCTTC 2 cut(s) 43, 469
HpyCH4III ACNGT 2 cut(s) 405, 440
HpyF10VI GCNNNNNNNGC 1 cut(s) 279
HpyF3I CTNAG 6 cut(s) 38, 175, 269, 306, 448, 497
Hsp92II CATG 3 cut(s) 54, 216, 470
Kzo9I GATC 3 cut(s) 355, 362, 427
LmnI GCTCC 3 cut(s) 187, 195, 313
Lsp1109I GCAGC 2 cut(s) 291, 315
LweI GCATC 1 cut(s) 196
MalI GATC 3 cut(s) 357, 364, 429
MboI GATC 3 cut(s) 355, 362, 427
MboII GAAGA 4 cut(s) 16, 68, 190, 472
MflI RGATCY 1 cut(s) 427
MhlI GDGCHC 2 cut(s) 192, 318
MlyI GAGTC 1 cut(s) 51
MseI TTAA 1 cut(s) 11
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 3 cut(s) 125, 138, 194
MvaI CCWGG 3 cut(s) 125, 138, 194
MwoI GCNNNNNNNGC 1 cut(s) 279
NcoI CCATGG 1 cut(s) 50
NdeII GATC 3 cut(s) 355, 362, 427
NlaIII CATG 3 cut(s) 54, 216, 470
NlaIV GGNNCC 1 cut(s) 429
PasI CCCWGGG 1 cut(s) 124
PfeI GAWTC 1 cut(s) 463
PkrI GCNGC 2 cut(s) 281, 330
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
Psp124BI GAGCTC 2 cut(s) 192, 318
Psp6I CCWGG 3 cut(s) 123, 136, 192
PspGI CCWGG 3 cut(s) 123, 136, 192
PspN4I GGNNCC 1 cut(s) 429
PspPI GGNCC 1 cut(s) 54
PstNI CAGNNNCTG 1 cut(s) 130
PsuI RGATCY 1 cut(s) 427
SacI GAGCTC 2 cut(s) 192, 318
SaqAI TTAA 1 cut(s) 11
SatI GCNGC 2 cut(s) 280, 329
Sau3AI GATC 3 cut(s) 355, 362, 427
Sau96I GGNCC 1 cut(s) 54
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 3 cut(s) 125, 138, 194
SduI GDGCHC 2 cut(s) 192, 318
SfaNI GCATC 1 cut(s) 196
SinI GGWCC 1 cut(s) 54
SstI GAGCTC 2 cut(s) 192, 318
StyD4I CCNGG 3 cut(s) 123, 136, 192
StyI CCWWGG 1 cut(s) 50
TaaI ACNGT 2 cut(s) 405, 440
TfiI GAWTC 1 cut(s) 463
Tru1I TTAA 1 cut(s) 11
Tru9I TTAA 1 cut(s) 11
TseI GCWGC 2 cut(s) 279, 328
VpaK11BI GGWCC 1 cut(s) 54
XagI CCTNNNNNAGG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.