Rh5AG251300

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012173
Physical Location & Seq
Forward (+)
32255365 .. 32257362
1998 bp
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UTR
Exon/CDS
Intron
Rh5AG251300.1

Sequence Viewer

Length: 477 bp
ATGTCCTTCTTCACACTACTCCTCTCATGGGCTTTCCTAACCATCCTTCTCTCTCTACCCCTTTCTCTCTCCTCTTCCTCCATCCACGACCTCCTCCGCTCGCAGGGCCTGCCAGCTGGTCTCCTCCCGAAGGAGGTCAAGTCCTACTCACTCTCCGACGATGGCTTCCTCCAAGTCTTCCTCGACGCTCCGTGCCTCACAAAATTCGAGAACCGGGTTTTCTTTGACAGCGTCGTTAGGGCCAACCTCAGCTACGGAAGTCTCATCGGAGTTGAGGGTTTGACTCAGGAAGAGCTCTTTCTGTGGCTGCCGGTGAAGGACATCATCGTTGATGATCCGGGATCAGGTCTCATACTTTTCGACATTGGAGTCGCTCATAAACAGCTTTCTCTGTCGCTCTTTGAAGATCCTCCTTCCTGTAAACCCTCAGGTTATTCTATCATGATATATCAGATCATCATTAGCTATTTAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

158

Amino Acids

17.44

Weight (kDa)

4.56

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 135 3.3e-28 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012173)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54530
fragaria_vesca FvH4_3g21500
malus_domestica MD01G1090100.v1.1 MD03G1215600.v1.1 MD11G1230200.v1.1
prunus_persica Prupe.4G197200_v2.0.a1
pyrus_communis pycom03g16400 pycom11g20400
rosa_chinensis RchiOBHm_Chr5g0036931
rosa_laevigata RLG00000033735
rosa_multiflora Rmu_sc0007663.1_g000018
rosa_roxburghii Rroxscaffold_1G00044020
rosa_rugosa Rorug05G0159000 Rorug05G0159100
rosa_samantha Rh5AG251300 Rh5BG252800 Rh5CG285400 Rh5DG261400
rosa_wichuraiana Rw5G023120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AccBSI CCGCTC 1 cut(s) 99
AciI CCGC 1 cut(s) 97
AclWI GGATC 3 cut(s) 329, 349, 401
AcsI RAATTY 1 cut(s) 203
AfiI CCNNNNNNNGG 5 cut(s) 28, 103, 130, 133, 344
AgsI TTSAA 1 cut(s) 404
AluBI AGCT 6 cut(s) 116, 252, 295, 385, 465, 473
AluI AGCT 6 cut(s) 116, 252, 295, 385, 465, 473
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 3 cut(s) 125, 266, 353
AlwI GGATC 3 cut(s) 329, 349, 401
AlwNI CAGNNNCTG 1 cut(s) 109
AoxI GGCC 2 cut(s) 106, 240
ApeKI GCWGC 1 cut(s) 307
ApoI RAATTY 1 cut(s) 203
AspS9I GGNCC 2 cut(s) 106, 240
AsuC2I CCSGG 2 cut(s) 215, 339
AsuHPI GGTGA 1 cut(s) 325
AxyI CCTNAGG 1 cut(s) 427
BanII GRGCYC 1 cut(s) 297
BbsI GAAGAC 1 cut(s) 169
Bbv12I GWGCWC 1 cut(s) 297
BbvCI CCTCAGC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 294
BccI CCATC 3 cut(s) 50, 89, 155
BcnI CCSGG 2 cut(s) 215, 339
BcoDI GTCTC 3 cut(s) 125, 266, 353
BisI GCNGC 1 cut(s) 308
BlsI GCNGC 1 cut(s) 309
Bme1390I CCNGG 2 cut(s) 215, 339
BmgT120I GGNCC 2 cut(s) 106, 240
BmrFI CCNGG 2 cut(s) 215, 339
BpiI GAAGAC 1 cut(s) 169
Bpu10I CCTNAGC 1 cut(s) 248
BpuMI CCSGG 2 cut(s) 215, 339
BsaI GGTCTC 2 cut(s) 125, 353
BsaXI ACNNNNNCTCC 4 cut(s) 125, 137, 155, 167
Bsc4I CCNNNNNNNGG 5 cut(s) 28, 103, 130, 133, 344
Bse118I RCCGGY 1 cut(s) 310
Bse21I CCTNAGG 1 cut(s) 427
BseGI GGATG 2 cut(s) 42, 81
BseLI CCNNNNNNNGG 5 cut(s) 28, 103, 130, 133, 344
BseMII CTCAG 3 cut(s) 262, 299, 441
BseRI GAGGAG 4 cut(s) 11, 61, 83, 113
BseXI GCAGC 1 cut(s) 294
BshFI GGCC 2 cut(s) 108, 242
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 3 cut(s) 214, 311, 338
BslI CCNNNNNNNGG 5 cut(s) 28, 103, 130, 133, 344
BsmAI GTCTC 3 cut(s) 125, 266, 353
BsnI GGCC 2 cut(s) 108, 242
Bso31I GGTCTC 2 cut(s) 125, 353
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 4 cut(s) 334, 341, 406, 453
BspACI CCGC 1 cut(s) 97
BspANI GGCC 2 cut(s) 108, 242
BspCNI CTCAG 3 cut(s) 261, 298, 440
BspHI TCATGA 1 cut(s) 441
BspPI GGATC 3 cut(s) 329, 349, 401
BspQI GCTCTTC 1 cut(s) 285
BspTNI GGTCTC 2 cut(s) 125, 353
BsrBI CCGCTC 1 cut(s) 99
BsrFI RCCGGY 1 cut(s) 310
BssAI RCCGGY 1 cut(s) 310
BssMI GATC 4 cut(s) 334, 341, 406, 453
Bst6I CTCTTC 2 cut(s) 79, 285
BstAPI GCANNNNNTGC 1 cut(s) 109
BstC8I GCNNGC 3 cut(s) 101, 110, 114
BstDEI CTNAG 4 cut(s) 248, 285, 427, 474
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 2 cut(s) 42, 81
BstKTI GATC 4 cut(s) 337, 344, 409, 456
BstMAI GTCTC 3 cut(s) 125, 266, 353
BstMBI GATC 4 cut(s) 334, 341, 406, 453
BstMWI GCNNNNNNNGC 2 cut(s) 105, 109
BstSCI CCNGG 2 cut(s) 213, 337
BstV1I GCAGC 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 169
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
Bsu36I CCTNAGG 1 cut(s) 427
BsuRI GGCC 2 cut(s) 108, 242
BtsCI GGATG 2 cut(s) 42, 81
Cac8I GCNNGC 3 cut(s) 101, 110, 114
CaiI CAGNNNCTG 1 cut(s) 109
CciI TCATGA 1 cut(s) 441
Cfr10I RCCGGY 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 106, 240
CseI GACGC 2 cut(s) 194, 220
CviAII CATG 2 cut(s) 27, 442
DdeI CTNAG 4 cut(s) 248, 285, 427, 474
DpnI GATC 4 cut(s) 336, 343, 408, 455
DpnII GATC 4 cut(s) 334, 341, 406, 453
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 79, 285
EarI CTCTTC 2 cut(s) 79, 285
Ecl136II GAGCTC 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 297
Eco31I GGTCTC 2 cut(s) 125, 353
Eco53kI GAGCTC 1 cut(s) 295
Eco81I CCTNAGG 1 cut(s) 427
EcoICRI GAGCTC 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 297
FaeI CATG 2 cut(s) 30, 445
FaiI YATR 5 cut(s) 28, 353, 378, 443, 448
FatI CATG 2 cut(s) 26, 441
Fnu4HI GCNGC 1 cut(s) 308
FokI GGATG 2 cut(s) 29, 68
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 308
GluI GCNGC 1 cut(s) 308
HaeIII GGCC 2 cut(s) 108, 242
HapII CCGG 3 cut(s) 214, 311, 338
HgaI GACGC 2 cut(s) 194, 220
Hin1II CATG 2 cut(s) 30, 445
HinfI GANTC 2 cut(s) 283, 369
HpaII CCGG 3 cut(s) 214, 311, 338
HphI GGTGA 1 cut(s) 325
Hpy166II GTNNAC 1 cut(s) 422
Hpy188I TCNGA 3 cut(s) 157, 269, 453
Hpy188III TCNNGA 4 cut(s) 127, 208, 287, 442
Hpy8I GTNNAC 1 cut(s) 422
Hpy99I CGWCG 3 cut(s) 161, 188, 236
HpyAV CCTTC 5 cut(s) 16, 56, 124, 310, 423
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 109
HpyF3I CTNAG 4 cut(s) 248, 285, 427, 474
Hsp92II CATG 2 cut(s) 30, 445
Kzo9I GATC 4 cut(s) 334, 341, 406, 453
LguI GCTCTTC 1 cut(s) 285
LmnI GCTCC 1 cut(s) 193
Lsp1109I GCAGC 1 cut(s) 294
MalI GATC 4 cut(s) 336, 343, 408, 455
MbiI CCGCTC 1 cut(s) 99
MboI GATC 4 cut(s) 334, 341, 406, 453
MboII GAAGA 4 cut(s) 66, 169, 302, 416
MflI RGATCY 1 cut(s) 406
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 1 cut(s) 203
MlyI GAGTC 2 cut(s) 277, 378
MmeI TCCRAC 1 cut(s) 180
MspA1I CMGCKG 1 cut(s) 116
MspI CCGG 3 cut(s) 214, 311, 338
MspR9I CCNGG 2 cut(s) 215, 339
MwoI GCNNNNNNNGC 2 cut(s) 105, 109
NciI CCSGG 2 cut(s) 215, 339
NdeII GATC 4 cut(s) 334, 341, 406, 453
NlaIII CATG 2 cut(s) 30, 445
PagI TCATGA 1 cut(s) 441
PciSI GCTCTTC 1 cut(s) 285
PflFI GACNNNGTC 1 cut(s) 230
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 1 cut(s) 309
PleI GAGTC 2 cut(s) 277, 377
PpsI GAGTC 2 cut(s) 277, 377
Psp124BI GAGCTC 1 cut(s) 297
PspPI GGNCC 2 cut(s) 106, 240
PstNI CAGNNNCTG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 406
PsyI GACNNNGTC 1 cut(s) 230
PvuII CAGCTG 1 cut(s) 116
SacI GAGCTC 1 cut(s) 297
SapI GCTCTTC 1 cut(s) 285
SatI GCNGC 1 cut(s) 308
Sau3AI GATC 4 cut(s) 334, 341, 406, 453
Sau96I GGNCC 2 cut(s) 106, 240
SchI GAGTC 2 cut(s) 277, 378
ScrFI CCNGG 2 cut(s) 215, 339
SduI GDGCHC 1 cut(s) 297
Sse9I AATT 1 cut(s) 203
SsiI CCGC 1 cut(s) 97
SstI GAGCTC 1 cut(s) 297
StyD4I CCNGG 2 cut(s) 213, 337
TaqI TCGA 3 cut(s) 183, 207, 360
TasI AATT 1 cut(s) 203
TseI GCWGC 1 cut(s) 307
TspGWI ACGGA 2 cut(s) 180, 270
Tth111I GACNNNGTC 1 cut(s) 230
XagI CCTNNNNNAGG 1 cut(s) 128
XapI RAATTY 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.