AT5G54630

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013269
Physical Location & Seq
Reverse (-)
22192367 .. 22194559
2193 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54630.1

Sequence Viewer

Length: 1419 bp
ATGGAAGATTCAAGCAAAAAAAGCCAAAAGAAGAAGAAGAACAAAAGCAAGAACAACATCCCAACTGTTTGGTTCTCACTCAAGAAATCCCTCCACTGTAAATCCGAACCATCTGATGTCCACGACCCAATATCCACCACTAAACAGCAACAACACCTCTCCACAATCTCCACCAAGAAAATCTCCGGCATATCATCCGGCGGAGCCGCCGTATGCGGTGGTGGTTTATCAGGATGTTCAAGATCAATAGCAAATCTCAAAGACGTAATCCATGGAAGCAAAAGACATTTCGAGAAACCACCAATAAGTAGTCCTCGTTCTATTGGAAGCAATGAGTTTCTAAATCCTATAACTCATGAAGTTATTCTTAGTAACTCAACTTGTGAGCTTAAGATCACTGGTGTCGGAGACATGGCTTCACCTGTCGGAGCAGCGGATTCCGGCGGAGGTGGCGGTGGAGGAAATGGTCGGTCTACGACTTATGTAGGGATGCTTAGACCAGGAACACCGATGCATTATCTTAACCATTCAGCTTCTTACAGGAGCCAAACAAGAAAGGGTTCGTTTGCTTTGTCGGAGAGAGATCGAGGAGGAGGAGGAGGAGGAGAAGGGTTAGGGTTTCATACAAACAGAAGGGTTTCATTGGAGATGAACAGAGAATCCACCATTAATGGAGGCAACAATTCCTCTGTTTCTTGTCATAAATGTGGTGAACAGTTCAATAAGCTTGAAGCTGCTGAAGCTCATCATCTCTCTAAACATGCCGTAACGGAGCTTGTAGAAGGAGATTCATCAAGAAAAATAGTGGAGATAATCTGCAGAACAAGCTGGTTAAAGTCAGAGAACCAATGTGGAAGAATCGATAGAGTTTTAAAAGTACACAACATGCAGAAGACATTAGCTAGATTCGAGGAATACAGAGAAACAGTTAAAATCAGAGCAAGCAAACTTCAGAAGAAGCATCCAAGATGTCTCGCAGACGGTAACGAGCTACTTAGGTTCCACGGAACCACCGTGGCTTGTGGTTTAGGTATAAACGGGTCAACAAGTGTTTGCACGGCGGAGAAATGTTGTGTCTGTCGGATTATACGAAACGGGTTCTCGTCGAAACGGGAGAAGAATAATGGGGTTGGTGTTTTCACGGCGTCTACTAGTGGAAGAGCGTTCGAGTCGATTTTGGTTAACGGTGGAGATGAGAGTGGAGATGTTGATCGGACGGTGAGGAAAGTGTTGATTGTGTGTAGGGTTATCGCCGGAAGAGTTCATCGGCCGGTGGAGAATGTGGAGGAGATGAATGGGTTGATGAGTGGGTTTGATTCTTTGGCTGGCAAAGTTGGTTTGTATACAAATGTGGAGGAGCTTTATTTGCTTAATCCTAAAGCTTTGCTTCCTTGCTTTGTGGTCATTTGCAAGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

472

Amino Acids

50.9

Weight (kDa)

9.45

Isoelectric Point (pI)

37.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54630
fragaria_vesca FvH4_3g21630 FvH4_3g21630
malus_domestica MD03G1214000.v1.1 MD11G1229000.v1.1
prunus_persica Prupe.4G200600_v2.0.a1 Prupe.4G200600_v2.0.a1
pyrus_communis pycom11g20330
rosa_chinensis RchiOBHm_Chr5g0037081
rosa_laevigata RLG00000033750
rosa_multiflora Rmu_ssc0000260.1_g000002
rosa_roxburghii Rroxscaffold_1G00043870
rosa_rugosa Rorug05G0160300
rosa_samantha Rh5AG252400 Rh5BG254200 Rh5CG286800 Rh5DG262700
rosa_wichuraiana Rw5G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 473, 1148, 1343
AciI CCGC 7 cut(s) 201, 207, 216, 434, 444, 453, 1061
AcoI YGGCCR 1 cut(s) 1268
AcuI CTGAAG 2 cut(s) 759, 935
AcyI GRCGYC 1 cut(s) 1145
AfaI GTAC 1 cut(s) 879
AflII CTTAAG 1 cut(s) 389
AgsI TTSAA 4 cut(s) 12, 240, 721, 731
AhlI ACTAGT 1 cut(s) 1151
AjnI CCWGG 1 cut(s) 499
AloI GAACNNNNNNTCC 2 cut(s) 644, 676
Alw26I GTCTC 2 cut(s) 402, 977
AoxI GGCC 1 cut(s) 1268
ApeKI GCWGC 2 cut(s) 431, 734
AseI ATTAAT 1 cut(s) 669
Asp700I GAANNNNTTC 3 cut(s) 363, 559, 637
AsuHPI GGTGA 3 cut(s) 411, 722, 1231
BbsI GAAGAC 1 cut(s) 899
BbvI GCAGC 2 cut(s) 443, 721
BccI CCATC 1 cut(s) 118
BceAI ACGGC 4 cut(s) 194, 749, 1074, 1158
BciT130I CCWGG 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 402, 977
BcuI ACTAGT 1 cut(s) 1151
BfaI CTAG 2 cut(s) 903, 1152
BfmI CTRYAG 1 cut(s) 817
BfrI CTTAAG 1 cut(s) 389
BisI GCNGC 3 cut(s) 207, 432, 735
BlsI GCNGC 3 cut(s) 208, 433, 736
Bme1390I CCNGG 1 cut(s) 501
BmiI GGNNCC 4 cut(s) 205, 545, 1001, 1009
BmrFI CCNGG 1 cut(s) 501
BmsI GCATC 3 cut(s) 480, 501, 970
BpiI GAAGAC 1 cut(s) 899
BpuEI CTTGAG 1 cut(s) 65
Bsa29I ATCGAT 1 cut(s) 861
BsaBI GATNNNNATC 1 cut(s) 1209
BsaHI GRCGYC 1 cut(s) 1145
BsaJI CCNNGG 3 cut(s) 271, 1003, 1014
BsaXI ACNNNNNCTCC 2 cut(s) 195, 225
Bse118I RCCGGY 1 cut(s) 1270
Bse1I ACTGG 1 cut(s) 403
Bse3DI GCAATG 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 1209
BseBI CCWGG 1 cut(s) 501
BseCI ATCGAT 1 cut(s) 861
BseDI CCNNGG 3 cut(s) 271, 1003, 1014
BseGI GGATG 5 cut(s) 57, 194, 239, 495, 961
BseJI GATNNNNATC 1 cut(s) 1209
BseMI GCAATG 1 cut(s) 337
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 8 cut(s) 603, 606, 609, 612, 615, 618, 1301, 1370
BseX3I CGGCCG 1 cut(s) 1268
BseXI GCAGC 2 cut(s) 443, 721
Bsh1285I CGRYCG 1 cut(s) 1271
BshFI GGCC 1 cut(s) 1270
BshVI ATCGAT 1 cut(s) 861
BsiEI CGRYCG 1 cut(s) 1271
BsiSI CCGG 5 cut(s) 186, 198, 441, 1254, 1271
BsmAI GTCTC 2 cut(s) 402, 977
BsnI GGCC 1 cut(s) 1270
Bsp143I GATC 4 cut(s) 242, 393, 583, 1210
Bsp19I CCATGG 1 cut(s) 271
BspACI CCGC 7 cut(s) 201, 207, 216, 434, 444, 453, 1061
BspANI GGCC 1 cut(s) 1270
BspDI ATCGAT 1 cut(s) 861
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 4 cut(s) 205, 545, 1001, 1009
BspMAI CTGCAG 1 cut(s) 821
BspQI GCTCTTC 1 cut(s) 1153
BspTI CTTAAG 1 cut(s) 389
BsrDI GCAATG 1 cut(s) 337
BsrFI RCCGGY 1 cut(s) 1270
BsrI ACTGG 1 cut(s) 403
BssAI RCCGGY 1 cut(s) 1270
BssECI CCNNGG 3 cut(s) 271, 1003, 1014
BssMI GATC 4 cut(s) 242, 393, 583, 1210
BssNAI GTATAC 1 cut(s) 1344
BssNI GRCGYC 1 cut(s) 1145
BssT1I CCWWGG 1 cut(s) 271
Bst1107I GTATAC 1 cut(s) 1344
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 8 cut(s) 67, 98, 717, 928, 983, 1015, 1187, 1219
Bst6I CTCTTC 2 cut(s) 1153, 1252
BstACI GRCGYC 1 cut(s) 1145
BstAFI CTTAAG 1 cut(s) 389
BstC8I GCNNGC 3 cut(s) 943, 1327, 1412
BstDEI CTNAG 3 cut(s) 368, 494, 995
BstDSI CCRYGG 3 cut(s) 271, 1003, 1014
BstF5I GGATG 5 cut(s) 57, 194, 239, 495, 961
BstKTI GATC 4 cut(s) 245, 396, 586, 1213
BstMAI GTCTC 2 cut(s) 402, 977
BstMBI GATC 4 cut(s) 242, 393, 583, 1210
BstMCI CGRYCG 1 cut(s) 1271
BstMWI GCNNNNNNNGC 5 cut(s) 21, 450, 740, 825, 1366
BstNI CCWGG 1 cut(s) 501
BstNSI RCATGY 2 cut(s) 764, 889
BstSCI CCNGG 1 cut(s) 499
BstSFI CTRYAG 1 cut(s) 817
BstV1I GCAGC 2 cut(s) 443, 721
BstV2I GAAGAC 1 cut(s) 899
BstXI CCANNNNNNTGG 1 cut(s) 69
BstZ17I GTATAC 1 cut(s) 1344
BstZI CGGCCG 1 cut(s) 1268
Bsu15I ATCGAT 1 cut(s) 861
BsuRI GGCC 1 cut(s) 1270
BsuTUI ATCGAT 1 cut(s) 861
BtgI CCRYGG 3 cut(s) 271, 1003, 1014
BtsCI GGATG 5 cut(s) 57, 194, 239, 495, 961
BtsIMutI CAGTG 2 cut(s) 94, 396
Cac8I GCNNGC 3 cut(s) 943, 1327, 1412
CciI TCATGA 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 1270
ClaI ATCGAT 1 cut(s) 861
CseI GACGC 1 cut(s) 1134
Csp6I GTAC 1 cut(s) 878
CviAII CATG 5 cut(s) 272, 356, 412, 761, 886
CviQI GTAC 1 cut(s) 878
DdeI CTNAG 3 cut(s) 368, 494, 995
DpnI GATC 4 cut(s) 244, 395, 585, 1212
DpnII GATC 4 cut(s) 242, 393, 583, 1210
DraI TTTAAA 1 cut(s) 873
EaeI YGGCCR 1 cut(s) 1268
EagI CGGCCG 1 cut(s) 1268
Eam1104I CTCTTC 2 cut(s) 1153, 1252
EarI CTCTTC 2 cut(s) 1153, 1252
EciI GGCGGA 3 cut(s) 216, 459, 1076
EclXI CGGCCG 1 cut(s) 1268
Eco130I CCWWGG 1 cut(s) 271
Eco52I CGGCCG 1 cut(s) 1268
Eco57I CTGAAG 2 cut(s) 759, 935
EcoRII CCWGG 1 cut(s) 499
EcoT14I CCWWGG 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 271
FaeI CATG 5 cut(s) 275, 359, 415, 764, 889
FalI AAGNNNNNCTT 4 cut(s) 351, 383, 1371, 1403
FatI CATG 5 cut(s) 271, 355, 411, 760, 885
FblI GTMKAC 3 cut(s) 473, 1148, 1343
Fnu4HI GCNGC 3 cut(s) 207, 432, 735
FokI GGATG 5 cut(s) 44, 181, 246, 502, 948
Fsp4HI GCNGC 3 cut(s) 207, 432, 735
FspBI CTAG 2 cut(s) 903, 1152
GluI GCNGC 3 cut(s) 207, 432, 735
HaeIII GGCC 1 cut(s) 1270
HapII CCGG 5 cut(s) 186, 198, 441, 1254, 1271
HgaI GACGC 1 cut(s) 1134
Hin1I GRCGYC 1 cut(s) 1145
Hin1II CATG 5 cut(s) 275, 359, 415, 764, 889
HincII GTYRAC 2 cut(s) 1044, 1183
HindII GTYRAC 2 cut(s) 1044, 1183
HindIII AAGCTT 2 cut(s) 725, 1380
HinfI GANTC 8 cut(s) 8, 437, 659, 788, 858, 906, 1169, 1316
HpaI GTTAAC 1 cut(s) 1183
HpaII CCGG 5 cut(s) 186, 198, 441, 1254, 1271
HphI GGTGA 3 cut(s) 411, 722, 1231
Hpy166II GTNNAC 8 cut(s) 121, 474, 713, 880, 1044, 1149, 1183, 1344
Hpy188III TCNNGA 6 cut(s) 82, 231, 240, 292, 356, 795
Hpy8I GTNNAC 8 cut(s) 121, 474, 713, 880, 1044, 1149, 1183, 1344
Hpy99I CGWCG 1 cut(s) 1108
HpyAV CCTTC 3 cut(s) 602, 627, 776
HpyCH4III ACNGT 8 cut(s) 67, 98, 717, 928, 983, 1015, 1187, 1219
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 5 cut(s) 514, 819, 889, 1056, 1410
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 450, 740, 825, 1366
HpyF3I CTNAG 3 cut(s) 368, 494, 995
HpySE526I ACGT 1 cut(s) 264
Hsp92I GRCGYC 1 cut(s) 1145
Hsp92II CATG 5 cut(s) 275, 359, 415, 764, 889
KspAI GTTAAC 1 cut(s) 1183
Kzo9I GATC 4 cut(s) 242, 393, 583, 1210
LguI GCTCTTC 1 cut(s) 1153
LmnI GCTCC 5 cut(s) 203, 428, 543, 772, 1357
Lsp1109I GCAGC 2 cut(s) 443, 721
LweI GCATC 3 cut(s) 480, 501, 970
MaeI CTAG 2 cut(s) 903, 1152
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 3 cut(s) 371, 766, 983
MalI GATC 4 cut(s) 244, 395, 585, 1212
MboI GATC 4 cut(s) 242, 393, 583, 1210
MluCI AATT 1 cut(s) 682
MlyI GAGTC 1 cut(s) 1178
MmeI TCCRAC 4 cut(s) 385, 406, 555, 1061
Mph1103I ATGCAT 1 cut(s) 516
MroXI GAANNNNTTC 3 cut(s) 363, 559, 637
MseI TTAA 8 cut(s) 390, 522, 669, 833, 872, 930, 1182, 1371
MslI CAYNNNNRTG 1 cut(s) 705
MspA1I CMGCKG 1 cut(s) 434
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 5 cut(s) 186, 198, 441, 1254, 1271
MspR9I CCNGG 1 cut(s) 501
MvaI CCWGG 1 cut(s) 501
MwoI GCNNNNNNNGC 5 cut(s) 21, 450, 740, 825, 1366
NcoI CCATGG 1 cut(s) 271
NdeII GATC 4 cut(s) 242, 393, 583, 1210
NlaIII CATG 5 cut(s) 275, 359, 415, 764, 889
NlaIV GGNNCC 4 cut(s) 205, 545, 1001, 1009
NsiI ATGCAT 1 cut(s) 516
NspI RCATGY 2 cut(s) 764, 889
PagI TCATGA 1 cut(s) 355
PciSI GCTCTTC 1 cut(s) 1153
PcsI WCGNNNNNNNCGW 2 cut(s) 1011, 1087
PdmI GAANNNNTTC 3 cut(s) 363, 559, 637
PfeI GAWTC 7 cut(s) 8, 437, 659, 788, 858, 906, 1316
PkrI GCNGC 3 cut(s) 208, 433, 736
PleI GAGTC 1 cut(s) 1177
PpsI GAGTC 1 cut(s) 1177
PshBI ATTAAT 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 499
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 4 cut(s) 205, 545, 1001, 1009
PsrI GAACNNNNNNTAC 2 cut(s) 301, 333
PstI CTGCAG 1 cut(s) 821
RsaI GTAC 1 cut(s) 879
RsaNI GTAC 1 cut(s) 878
RseI CAYNNNNRTG 1 cut(s) 705
SapI GCTCTTC 1 cut(s) 1153
SaqAI TTAA 8 cut(s) 390, 522, 669, 833, 872, 930, 1182, 1371
SatI GCNGC 3 cut(s) 207, 432, 735
Sau3AI GATC 4 cut(s) 242, 393, 583, 1210
SchI GAGTC 1 cut(s) 1178
ScrFI CCNGG 1 cut(s) 501
SfaNI GCATC 3 cut(s) 480, 501, 970
SfcI CTRYAG 1 cut(s) 817
SmiMI CAYNNNNRTG 1 cut(s) 705
SmlI CTYRAG 2 cut(s) 80, 389
SmoI CTYRAG 2 cut(s) 80, 389
SpeI ACTAGT 1 cut(s) 1151
Sse9I AATT 1 cut(s) 682
SsiI CCGC 7 cut(s) 201, 207, 216, 434, 444, 453, 1061
SspMI CTAG 2 cut(s) 903, 1152
StyD4I CCNGG 1 cut(s) 499
StyI CCWWGG 1 cut(s) 271
TaaI ACNGT 8 cut(s) 67, 98, 717, 928, 983, 1015, 1187, 1219
TaiI ACGT 1 cut(s) 267
TaqI TCGA 7 cut(s) 291, 586, 861, 909, 1106, 1167, 1172
TaqII GACCGA 1 cut(s) 459
TasI AATT 1 cut(s) 682
TatI WGTACW 1 cut(s) 877
TauI GCSGC 1 cut(s) 209
TfiI GAWTC 7 cut(s) 8, 437, 659, 788, 858, 906, 1316
Tru1I TTAA 8 cut(s) 390, 522, 669, 833, 872, 930, 1182, 1371
Tru9I TTAA 8 cut(s) 390, 522, 669, 833, 872, 930, 1182, 1371
TscAI CASTG 2 cut(s) 101, 403
TseI GCWGC 2 cut(s) 431, 734
TspDTI ATGAA 7 cut(s) 372, 611, 630, 665, 780, 1253, 1307
TspGWI ACGGA 2 cut(s) 785, 1020
TspRI CASTG 2 cut(s) 101, 403
Vha464I CTTAAG 1 cut(s) 389
VspI ATTAAT 1 cut(s) 669
XceI RCATGY 2 cut(s) 764, 889
XmiI GTMKAC 3 cut(s) 473, 1148, 1343
XmnI GAANNNNTTC 3 cut(s) 363, 559, 637
XspI CTAG 2 cut(s) 903, 1152
Zsp2I ATGCAT 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.