Rh5AG252400

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013269
Physical Location & Seq
Reverse (-)
32391386 .. 32394279
2894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG252400.1

Sequence Viewer

Length: 1305 bp
ATGCCAACAGTGTGGTTCTCTCTGAAGAAGTCTCTCCACTGCAAGTCAGAGCCCTCAGATGTTCATGACCCAAAGTCAAGGAAGCAGTTGACCACAATCCTGACCAGAAAGCCAGGAGGGGGGAGGTCAGGTTGTTCAAGGTCCATAGCAAATCTCAAGGATGTGATTCATGGAAGCAAGAGACATATGGAGAGGCCCCCAAGTAACTGCAGCCCAAGATCCATAGGGAGCAGTGAGTTCCTCAACCCAATAACCCATGAAGTCATTTTGAGCAACTCAAGGTGTGAGCTCAAAATCACTAGCTTTGATATTGGCAATGGCCACAGCAACAATGGAGGTGGGAATAGCAGTAATGGTGGTTCAACATTTGCGGATACCCTCAAGCCAGGCACACCTGGGCCTGGAGGCCACCCCACAATGCACTATTTCAACCCTTCTTTTAGGACCTCAGCCACTACTCCTCCAAGGAAGAGCCCTTTTCGGGTTTCTGCAGAAACAGAAGGCTCTGGAAATTTTAAGGGTTCTTCAGGTTTCAATTCAGCTAGTGGGATTGGACTTCGCCAAAGCAATAGGATCTCTTTTGATGGTTCTACCTCAATTACTTGTCACAAATGTGGTGAGCAGTTTACAAAATGGGAAGCTGCTGAAGCACACCATCTCTCTAAACATGCTGTTACTGAACTTATGGAAGGTGACTCGTCCAGAAAAATCGTTGAGATAATATGCCGGACAAGCTGGTTGAAATCCGAGAACAATTGTGGAAGGATTGAGAGAGTGTTGAAAGTGCACAACATGCAAAGAACTCTTGCTCGATTTGAAGAATATCGAGAGATGGTGAAGATCAAAGCCAGCAAACTCCCTAAGAAACACCCAAGGTGCATTGCTGATGGGAATGAACTTTTGAGGTTTTATGGGACTACAGTGGCCTGCTCTCTTGGCCTAAATAGTTCCTCAAGTCTTTGTGTATCAGAAAAATGCTGTGTTTGTAGGATTATAAGAAATGGGTTCTGTGCAAAGAAGGAGCTCAAGGAAGGTGTAGGTGTTTTCACAACTTCCACAAGCGCAAGAGCCTATGAATGCATTGAAATATTGGAAGAGGATCCATGTATTAGGAAAGCTTTGATAGTCTGCAGAGTAATTGCTGGGAGGGTTCATAAGCCTTTGGAGAACATAAAAGAAATTGCTGGACAAACTGGTTTTGATTCTTTGGCTGGAAAAATGGGTCTTTACTCAAATATTGAAGAGCTTTATCTGCTCAATCCTAGAGCTCTCCTTCCTTGCTTTGTGGTAATCTGCAAACCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

434

Amino Acids

47.55

Weight (kDa)

9.31

Isoelectric Point (pI)

43.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013269)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54630
fragaria_vesca FvH4_3g21630 FvH4_3g21630
malus_domestica MD03G1214000.v1.1 MD11G1229000.v1.1
prunus_persica Prupe.4G200600_v2.0.a1 Prupe.4G200600_v2.0.a1
pyrus_communis pycom11g20330
rosa_chinensis RchiOBHm_Chr5g0037081
rosa_laevigata RLG00000033750
rosa_multiflora Rmu_ssc0000260.1_g000002
rosa_roxburghii Rroxscaffold_1G00043870
rosa_rugosa Rorug05G0160300
rosa_samantha Rh5AG252400 Rh5BG254200 Rh5CG286800 Rh5DG262700
rosa_wichuraiana Rw5G023290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 995
AciI CCGC 1 cut(s) 371
AclWI GGATC 4 cut(s) 213, 581, 1094, 1107
AcoI YGGCCR 1 cut(s) 319
AcsI RAATTY 1 cut(s) 511
AcuI CTGAAG 3 cut(s) 44, 510, 666
AfiI CCNNNNNNNGG 3 cut(s) 119, 401, 481
AgsI TTSAA 9 cut(s) 138, 363, 430, 535, 742, 781, 818, 1085, 1241
AhdI GACNNNNNGTC 1 cut(s) 73
AjnI CCWGG 4 cut(s) 112, 385, 394, 400
AleI CACNNNNGTG 1 cut(s) 612
AluBI AGCT 9 cut(s) 289, 303, 542, 641, 735, 1024, 1118, 1246, 1268
AluI AGCT 9 cut(s) 289, 303, 542, 641, 735, 1024, 1118, 1246, 1268
Alw21I GWGCWC 4 cut(s) 291, 789, 1026, 1270
Alw26I GTCTC 2 cut(s) 36, 175
Alw44I GTGCAC 1 cut(s) 785
AlwI GGATC 4 cut(s) 213, 581, 1094, 1107
AoxI GGCC 6 cut(s) 194, 319, 398, 406, 924, 937
ApaLI GTGCAC 1 cut(s) 785
ApeKI GCWGC 2 cut(s) 210, 641
ApoI RAATTY 1 cut(s) 511
AspLEI GCGC 1 cut(s) 1064
AspS9I GGNCC 4 cut(s) 141, 195, 398, 444
AsuHPI GGTGA 3 cut(s) 629, 704, 847
AvaII GGWCC 2 cut(s) 141, 444
BaeGI GKGCMC 1 cut(s) 789
BalI TGGCCA 1 cut(s) 321
BamHI GGATCC 1 cut(s) 1099
BanII GRGCYC 5 cut(s) 54, 291, 476, 1026, 1270
Bbv12I GWGCWC 4 cut(s) 291, 789, 1026, 1270
BbvCI CCTCAGC 1 cut(s) 448
BbvI GCAGC 2 cut(s) 222, 628
BccI CCATC 4 cut(s) 578, 663, 826, 881
BciT130I CCWGG 4 cut(s) 114, 387, 396, 402
BciVI GTATCC 1 cut(s) 367
BcoDI GTCTC 2 cut(s) 36, 175
BfaI CTAG 3 cut(s) 300, 543, 1263
BfmI CTRYAG 4 cut(s) 208, 489, 918, 1129
BfuI GTATCC 1 cut(s) 367
BisI GCNGC 2 cut(s) 211, 642
BlsI GCNGC 2 cut(s) 212, 643
Bme1390I CCNGG 4 cut(s) 114, 387, 396, 402
Bme18I GGWCC 2 cut(s) 141, 444
BmeRI GACNNNNNGTC 1 cut(s) 73
BmgT120I GGNCC 4 cut(s) 141, 195, 398, 444
BmiI GGNNCC 2 cut(s) 197, 1101
BmrFI CCNGG 4 cut(s) 114, 387, 396, 402
BpmI CTGGAG 1 cut(s) 423
Bpu10I CCTNAGC 1 cut(s) 448
BpuEI CTTGAG 5 cut(s) 140, 262, 365, 937, 1010
BsaJI CCNNGG 3 cut(s) 395, 464, 872
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 445, 475
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 401, 481
Bse1I ACTGG 1 cut(s) 1198
Bse3DI GCAATG 2 cut(s) 322, 879
BseBI CCWGG 4 cut(s) 114, 387, 396, 402
BseDI CCNNGG 3 cut(s) 395, 464, 872
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 3 cut(s) 119, 401, 481
BseMI GCAATG 2 cut(s) 322, 879
BseMII CTCAG 2 cut(s) 69, 462
BseNI ACTGG 1 cut(s) 1198
BseRI GAGGAG 1 cut(s) 450
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 2 cut(s) 222, 628
BseYI CCCAGC 1 cut(s) 1142
BshFI GGCC 6 cut(s) 196, 321, 400, 408, 926, 939
BsiHKAI GWGCWC 4 cut(s) 291, 789, 1026, 1270
BsiSI CCGG 1 cut(s) 727
BslFI GGGAC 1 cut(s) 928
BslI CCNNNNNNNGG 3 cut(s) 119, 401, 481
BsmAI GTCTC 2 cut(s) 36, 175
BsmFI GGGAC 1 cut(s) 928
BsmI GAATGC 1 cut(s) 1082
BsnI GGCC 6 cut(s) 196, 321, 400, 408, 926, 939
Bsp1286I GDGCHC 6 cut(s) 54, 291, 476, 789, 1026, 1270
Bsp143I GATC 4 cut(s) 218, 573, 840, 1099
BspACI CCGC 1 cut(s) 371
BspANI GGCC 6 cut(s) 196, 321, 400, 408, 926, 939
BspCNI CTCAG 2 cut(s) 68, 461
BspHI TCATGA 1 cut(s) 64
BspLI GGNNCC 2 cut(s) 197, 1101
BspMAI CTGCAG 3 cut(s) 212, 493, 1133
BspPI GGATC 4 cut(s) 213, 581, 1094, 1107
BspQI GCTCTTC 2 cut(s) 464, 1236
BsrDI GCAATG 2 cut(s) 322, 879
BsrI ACTGG 1 cut(s) 1198
BssECI CCNNGG 3 cut(s) 395, 464, 872
BssMI GATC 4 cut(s) 218, 573, 840, 1099
BssT1I CCWWGG 2 cut(s) 464, 872
Bst2UI CCWGG 4 cut(s) 114, 387, 396, 402
Bst4CI ACNGT 2 cut(s) 10, 922
Bst6I CTCTTC 3 cut(s) 464, 1089, 1236
BstAPI GCANNNNNTGC 1 cut(s) 793
BstC8I GCNNGC 2 cut(s) 850, 928
BstDEI CTNAG 3 cut(s) 55, 448, 861
BstF5I GGATG 1 cut(s) 166
BstHHI GCGC 1 cut(s) 1064
BstKTI GATC 4 cut(s) 221, 576, 843, 1102
BstMAI GTCTC 2 cut(s) 36, 175
BstMBI GATC 4 cut(s) 218, 573, 840, 1099
BstMWI GCNNNNNNNGC 5 cut(s) 647, 732, 793, 936, 1252
BstNI CCWGG 4 cut(s) 114, 387, 396, 402
BstNSI RCATGY 2 cut(s) 671, 796
BstSCI CCNGG 4 cut(s) 112, 385, 394, 400
BstSFI CTRYAG 4 cut(s) 208, 489, 918, 1129
BstSLI GKGCMC 1 cut(s) 789
BstV1I GCAGC 2 cut(s) 222, 628
BstX2I RGATCY 3 cut(s) 218, 573, 1099
BstXI CCANNNNNNTGG 1 cut(s) 12
BstYI RGATCY 3 cut(s) 218, 573, 1099
BsuI GTATCC 1 cut(s) 367
BsuRI GGCC 6 cut(s) 196, 321, 400, 408, 926, 939
BtsCI GGATG 1 cut(s) 166
BtsI GCAGTG 2 cut(s) 37, 238
BtsIMutI CAGTG 4 cut(s) 15, 37, 238, 927
Cac8I GCNNGC 2 cut(s) 850, 928
CciI TCATGA 1 cut(s) 64
CfoI GCGC 1 cut(s) 1064
Cfr13I GGNCC 4 cut(s) 141, 195, 398, 444
CspCI CAANNNNNGTGG 2 cut(s) 319, 354
CviAII CATG 6 cut(s) 65, 170, 257, 668, 793, 1104
DdeI CTNAG 3 cut(s) 55, 448, 861
DpnI GATC 4 cut(s) 220, 575, 842, 1101
DpnII GATC 4 cut(s) 218, 573, 840, 1099
DriI GACNNNNNGTC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 3 cut(s) 464, 1089, 1236
Eam1105I GACNNNNNGTC 1 cut(s) 73
EarI CTCTTC 3 cut(s) 464, 1089, 1236
Ecl136II GAGCTC 3 cut(s) 289, 1024, 1268
Eco130I CCWWGG 2 cut(s) 464, 872
Eco24I GRGCYC 5 cut(s) 54, 291, 476, 1026, 1270
Eco47I GGWCC 2 cut(s) 141, 444
Eco53kI GAGCTC 3 cut(s) 289, 1024, 1268
Eco57I CTGAAG 3 cut(s) 44, 510, 666
EcoICRI GAGCTC 3 cut(s) 289, 1024, 1268
EcoO109I RGGNCCY 2 cut(s) 195, 444
EcoRII CCWGG 4 cut(s) 112, 385, 394, 400
EcoT14I CCWWGG 2 cut(s) 464, 872
EcoT22I ATGCAT 1 cut(s) 1082
EcoT38I GRGCYC 5 cut(s) 54, 291, 476, 1026, 1270
ErhI CCWWGG 2 cut(s) 464, 872
FaeI CATG 6 cut(s) 68, 173, 260, 671, 796, 1107
FaqI GGGAC 1 cut(s) 928
FatI CATG 6 cut(s) 64, 169, 256, 667, 792, 1103
FauNDI CATATG 1 cut(s) 186
Fnu4HI GCNGC 2 cut(s) 211, 642
FokI GGATG 1 cut(s) 173
FriOI GRGCYC 5 cut(s) 54, 291, 476, 1026, 1270
Fsp4HI GCNGC 2 cut(s) 211, 642
FspBI CTAG 3 cut(s) 300, 543, 1263
GlaI GCGC 1 cut(s) 1063
GluI GCNGC 2 cut(s) 211, 642
GsaI CCCAGC 1 cut(s) 1146
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 6 cut(s) 196, 321, 400, 408, 926, 939
HapII CCGG 1 cut(s) 727
HhaI GCGC 1 cut(s) 1064
Hin1II CATG 6 cut(s) 68, 173, 260, 671, 796, 1107
Hin6I GCGC 1 cut(s) 1062
HinP1I GCGC 1 cut(s) 1062
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 1116
HinfI GANTC 3 cut(s) 166, 695, 1202
HpaII CCGG 1 cut(s) 727
HphI GGTGA 3 cut(s) 629, 704, 847
Hpy166II GTNNAC 3 cut(s) 90, 627, 787
Hpy188I TCNGA 5 cut(s) 24, 49, 58, 748, 970
Hpy188III TCNNGA 5 cut(s) 65, 100, 507, 702, 827
Hpy8I GTNNAC 3 cut(s) 90, 627, 787
HpyAV CCTTC 7 cut(s) 444, 494, 683, 756, 1012, 1025, 1283
HpyCH4III ACNGT 2 cut(s) 10, 922
HpyF10VI GCNNNNNNNGC 5 cut(s) 647, 732, 793, 936, 1252
HpyF3I CTNAG 3 cut(s) 55, 448, 861
Hsp92II CATG 6 cut(s) 68, 173, 260, 671, 796, 1107
HspAI GCGC 1 cut(s) 1062
Kzo9I GATC 4 cut(s) 218, 573, 840, 1099
LguI GCTCTTC 2 cut(s) 464, 1236
LmnI GCTCC 2 cut(s) 228, 1021
Lsp1109I GCAGC 2 cut(s) 222, 628
MaeI CTAG 3 cut(s) 300, 543, 1263
MaeIII GTNAC 4 cut(s) 203, 605, 673, 692
MalI GATC 4 cut(s) 220, 575, 842, 1101
MboI GATC 4 cut(s) 218, 573, 840, 1099
MboII GAAGA 7 cut(s) 37, 481, 516, 830, 850, 1106, 1253
MfeI CAATTG 1 cut(s) 754
MflI RGATCY 3 cut(s) 218, 573, 1099
MhlI GDGCHC 6 cut(s) 54, 291, 476, 789, 1026, 1270
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 6 cut(s) 511, 535, 597, 754, 1137, 1179
MluNI TGGCCA 1 cut(s) 321
MlyI GAGTC 1 cut(s) 689
Mox20I TGGCCA 1 cut(s) 321
Mph1103I ATGCAT 1 cut(s) 1082
MscI TGGCCA 1 cut(s) 321
MseI TTAA 1 cut(s) 516
MslI CAYNNNNRTG 1 cut(s) 612
Msp20I TGGCCA 1 cut(s) 321
MspI CCGG 1 cut(s) 727
MspR9I CCNGG 4 cut(s) 114, 387, 396, 402
MunI CAATTG 1 cut(s) 754
Mva1269I GAATGC 1 cut(s) 1082
MvaI CCWGG 4 cut(s) 114, 387, 396, 402
MwoI GCNNNNNNNGC 5 cut(s) 647, 732, 793, 936, 1252
NdeI CATATG 1 cut(s) 186
NdeII GATC 4 cut(s) 218, 573, 840, 1099
NlaIII CATG 6 cut(s) 68, 173, 260, 671, 796, 1107
NlaIV GGNNCC 2 cut(s) 197, 1101
NmuCI GTSAC 2 cut(s) 605, 692
NsiI ATGCAT 1 cut(s) 1082
NspI RCATGY 2 cut(s) 671, 796
OliI CACNNNNGTG 1 cut(s) 612
PagI TCATGA 1 cut(s) 64
PciSI GCTCTTC 2 cut(s) 464, 1236
PctI GAATGC 1 cut(s) 1082
PfeI GAWTC 2 cut(s) 166, 1202
PkrI GCNGC 2 cut(s) 212, 643
PleI GAGTC 1 cut(s) 689
PpsI GAGTC 1 cut(s) 689
PpuMI RGGWCCY 1 cut(s) 444
PsiI TTATAA 1 cut(s) 995
Psp124BI GAGCTC 3 cut(s) 291, 1026, 1270
Psp5II RGGWCCY 1 cut(s) 444
Psp6I CCWGG 4 cut(s) 112, 385, 394, 400
PspFI CCCAGC 1 cut(s) 1142
PspGI CCWGG 4 cut(s) 112, 385, 394, 400
PspN4I GGNNCC 2 cut(s) 197, 1101
PspPI GGNCC 4 cut(s) 141, 195, 398, 444
PspPPI RGGWCCY 1 cut(s) 444
PsrI GAACNNNNNNTAC 2 cut(s) 343, 375
PstI CTGCAG 3 cut(s) 212, 493, 1133
PsuI RGATCY 3 cut(s) 218, 573, 1099
RseI CAYNNNNRTG 1 cut(s) 612
SacI GAGCTC 3 cut(s) 291, 1026, 1270
SapI GCTCTTC 2 cut(s) 464, 1236
SaqAI TTAA 1 cut(s) 516
SatI GCNGC 2 cut(s) 211, 642
Sau3AI GATC 4 cut(s) 218, 573, 840, 1099
Sau96I GGNCC 4 cut(s) 141, 195, 398, 444
SchI GAGTC 1 cut(s) 689
ScrFI CCNGG 4 cut(s) 114, 387, 396, 402
SduI GDGCHC 6 cut(s) 54, 291, 476, 789, 1026, 1270
SfcI CTRYAG 4 cut(s) 208, 489, 918, 1129
SinI GGWCC 2 cut(s) 141, 444
SmiMI CAYNNNNRTG 1 cut(s) 612
SmlI CTYRAG 5 cut(s) 155, 277, 380, 952, 1025
SmoI CTYRAG 5 cut(s) 155, 277, 380, 952, 1025
Sse9I AATT 6 cut(s) 511, 535, 597, 754, 1137, 1179
SsiI CCGC 1 cut(s) 371
SspI AATATT 2 cut(s) 1089, 1237
SspMI CTAG 3 cut(s) 300, 543, 1263
SstI GAGCTC 3 cut(s) 291, 1026, 1270
StyD4I CCNGG 4 cut(s) 112, 385, 394, 400
StyI CCWWGG 2 cut(s) 464, 872
TaaI ACNGT 2 cut(s) 10, 922
TaqI TCGA 2 cut(s) 811, 826
TasI AATT 6 cut(s) 511, 535, 597, 754, 1137, 1179
TfiI GAWTC 2 cut(s) 166, 1202
Tru1I TTAA 1 cut(s) 516
Tru9I TTAA 1 cut(s) 516
TscAI CASTG 4 cut(s) 15, 44, 238, 927
TseFI GTSAC 2 cut(s) 605, 692
TseI GCWGC 2 cut(s) 210, 641
Tsp45I GTSAC 2 cut(s) 605, 692
TspDTI ATGAA 6 cut(s) 53, 158, 273, 909, 1089, 1142
TspRI CASTG 4 cut(s) 15, 44, 238, 927
VneI GTGCAC 1 cut(s) 785
VpaK11BI GGWCC 2 cut(s) 141, 444
XapI RAATTY 1 cut(s) 511
XceI RCATGY 2 cut(s) 671, 796
XcmI CCANNNNNNNNNTGG 1 cut(s) 329
XspI CTAG 3 cut(s) 300, 543, 1263
Zsp2I ATGCAT 1 cut(s) 1082
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.